This document provides an introduction to immunology and the immune system. It discusses the study of immunology and defines the immune system. It also describes the innate and adaptive immune responses, natural and artificial immunity, the roles of antibodies and lymphocytes in humoral and cell-mediated immunity, and the functions of the lymphatic system. Key cell types like phagocytes, B cells, T cells, and antigens are also introduced.
By DR. MANPREET KAUR BEHL.
Description of classificaton of immune system, immune cells, HLA, MHC complexes, antigen presentation, t-cell responses and b-cell responses, antibody, isotype switching, hypersenstivity reactions etc.
By DR. MANPREET KAUR BEHL.
Description of classificaton of immune system, immune cells, HLA, MHC complexes, antigen presentation, t-cell responses and b-cell responses, antibody, isotype switching, hypersenstivity reactions etc.
introduction of adaptive immunity. classification of adaptive immunity, factor affecting it and mechanism of adaptive immunity comparison between adaptive immunity and innate immunity. characteristic of adaptive immunity . cell mediated immune responses immunoglobulins
types of immunoglobulins. functions of immunoglobulins, hypersensitivity reactions
introduction of adaptive immunity. classification of adaptive immunity, factor affecting it and mechanism of adaptive immunity comparison between adaptive immunity and innate immunity. characteristic of adaptive immunity . cell mediated immune responses immunoglobulins
types of immunoglobulins. functions of immunoglobulins, hypersensitivity reactions
This is the introductory immunology lecture that I created and presented as part of the Introductory Biology 10.010 course for Singapore University of Technology and Design. This presentation was for a 90 minute lecture for freshman non-major students.
Demonstrate knowledge of a particular body system. Describe the bodily components of the system; describe the pathology of the system; and teach word components and abbreviations.
Introduction to the topographical anatomy and operative sugerykavanvyas1
this short note contains all the necessary information about the basics of topographical anatomy and surgery , which is very helpful to beginners , especially medical aspirants.
The thorax refers to the region which forms a major part of the appendicular skeleton. Knowledge of its surface anatomy is essential for surgical techniques, to say the least.
SEMINAR BASICS OF IMMUNOLOGY- Antigens antibodies immunoglobulins and comple...DrShinyKajal
The basics of Immunology consisting of -
1. BASIC DEFINITIONS
2. HISTORY OF IMMUNOLOGY
3. ANATOMY, PHYSIOLOGY & PATHOLOGY OF IMMUNOLOGY
4- TYPES OF IMMUNITY (including COMPLEMENT SYSTEM)
5- CELLS AND TISSUES OF IMMUNE SYSTEM
6-ANTIGENS AND ANTIBODIES
7- IMMUNOGLOBULINS
8- MHC AND CYTOKINES
White blood cells & Immunity (The Guyton and Hall Physiology)Maryam Fida
Leukocytes or WBCs are the mobile units of the body’s immune defense system.
Immunity is the body’s ability to resist or eliminate potentially harmful foreign materials or abnormal cells.
WBC count: 5000 to 11000/ul of blood
GRANULOCYTES
Polymorphonuclear neutrophils 60-70%
Polymorphonuclear eosinophils 2-3%
Polymorphonuclear basophils 0.4%
NON-GRANULOCYTES
Monocytes 5.3%
Lymphocytes 30%
Granulocytes and monocytes are formed and stored only in bone marrow
Lymphocytes and plasma cells are formed and stored mainly in various lymphoid tissue such as lymph node, spleen, thymus and tonsils as well as in bone marrow.
GRANULOCYTES
4 to 8 hours in blood and 4 to 5 days in tissues
MONOCYTES
Monocytes also have a short transit time:
10 to 20 hours in blood and In tissue they swell to much larger size to become tissue macrophages.
LYMPHOCYTES
weeks to months
neutrophil
. 60-70% of leukocytes
nucleus: 2-5 lobes
Counting the number of lobes and grouping them is called Arneth count.
Shift to left means (increase no of young and predominant WBCs) e.g During acute infection.
Shift to right means, old cells are predominant. e.g During recovery phase
NEUTROPENIA
Decrease in neutrophils count
Typhoid
AIDS and viral hepatitis
Kalazar fever
Bone marrow depression by drugs and radiations
NEUTROPHILIA
Increase in neutrophils count
Appendicitis , Tonsillitis, Pneumonia
Burns, Hemorrhage, MI, Pain
Hypoxia, Pregnancy
BASOPHIL
Their cytoplasmic granules take up basic dyes and appear deep blue
MAST CELLS are derived from basophils under the influence of interleukins 3 and 4
Under many allergic conditions basophils and mast cells bursts and releases
Histamine
Bradykinin
Serotonin
Slow reacting substance of anaphylaxis
Heparin
Lysosomal enzymes
It is the capacity of the human body to resist and destroy the invading organisms or toxins.
immunity, types,Innate immunity and Adaptive Immunity, primary and secondary immune response, structure and functions of antibodies, immunoglobulins, hypergammaglobulinemia, multiple myeloma, bence jones protein, electrophoretic pattern of multiple myeloma.
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Richard's entangled aventures in wonderlandRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
2. IMMUNOLOGY AND THE IMMUNE
SYSTEM
* Immunology
* Study of the components and function of the immune
system
* Immune System
* Molecules, cells, tissues and organs which provide non-specific
and specific protection against
* Microorganisms
* Microbial toxins
* Tumor cells
* Crucial to human survival
3. THE IMMUNE RESPONSE AND
IMMUNITY
* Immune response
* Innate (non-specific)
* Adaptive (specific)
* Primary
* Secondary
* Immunity
* State of non-specific and specific protection
* Acquisition of Immunity
* Natural
* Artificial
4. NATURALLY ACQUIRED
IMMUNITY
* Active
* Antigens enter body naturally with response of
* Innate and adaptive immune systems
* Provides long term protection
* Passive
* Antibodies pass from mother to
* Fetus across placenta
* Infant in breast milk
* Provides immediate short term protection
5. ARTIFICIALLY ACQUIRED
IMMUNITY
* Active
* Antigens enter body through vaccination with response of
* Innate and adaptive immune systems
* Provides long term protection
* Passive
* Antibodies from immune individuals injected into body
* Referred to as
* Immune serum globulins (ISG)
* Immune globulins (IG)
* Gamma globulins
* Provides immediate short term protection
6. PRINCIPAL FUNCTION OF THE
IMMUNE SYSTEM
* To protect humans from pathogenic microorganisms
* Pathogenic microorganisms (Pathogens)
* Microorganisms capable of causing infection and/or
disease
* Infection
* Ability of pathogen to enter host, multiply and
stimulate an immune response
* Disease
* Clinical manifestations associated with infection
8. DEFENSE MECHANISMS OF THE
HUMAN HOST
* Innate Mechanisms (Innate immunity)
* First line of defense
* Non-specific
* Adaptive Mechanisms (Adaptive immunity)
* Second line of defense
* Highly specific with memory
* Cooperation between mechanisms
9. THE CLUSTER OF
DIFFERENTIATION (CD)
* A protocol for identification and investigation of cell
surface molecules
* CD number assigned on basis of 1 cell surface molecule
recognized by 2 specific monoclonal antibodies
* CD nomenclature established in 1982
* 1st International Workshop and Conference on Human
Leukocyte Differentiation Antigens (HLDA)
10. THE CLUSTER OF
DIFFERENTIATION (CD)
* CD markers on leukocytes
Granulocyte CD45+, CD15+
Monocyte CD45+, CD14+
T lymphocyte CD45+, CD3+
T helper lymphocyte CD45+, CD3+, CD4+
T cytotoxic lymphocyte CD45+, CD3+, CD8+
B lymphocyte CD45+, CD19+
Natural killer cell CD45+, CD16+, CD56+, CD3-
11. THE LYMPHATIC SYSTEM
* Lymph
* Fluid and cells in lymphatic vessels
* Lymphatic vessels
* Collect and return interstitial fluid to blood
* Transport immune cells throughout body
* Transport lipid from intestine to blood
* Lymph nodes
* Kidney shaped organs at intervals along lymphatic vessels
* Other secondary lymphatic tissues and organs
12.
13. LYMPHOCYTES AND THE LYMPH
NODES
* Naïve lymphocytes circulate between blood, lymph and
secondary lymph nodes
* Pathogens from infected tissue sites are picked up by
lymphatic vessels and arrive at closest lymph node
* T and B cells congregate at specific regions of nodes
* Architecture and size of nodes change in response to
activation of lymphocytes
14.
15.
16. LYMPHOCYTES AND THE SPLEEN
* Spleen
* Lymphoid organ in upper left abdomen
* Functions
* Remove damaged or old erythrocytes
* Activation of lymphocytes from blood borne pathogens
* Architecture of Spleen
* Red pulp
* Erythrocytes removed
* White pulp
* Lymphocytes stimulated
17.
18. SECONDARY LYMPHOID TISSUES
ASSOCIATED WITH MUCOUS
MEMBRANES
* Primary portals of entry for pathogens
* Respiratory tract
* Gastrointestinal tract
* Secondary lymphoid tissues
* Bronchial-associated lymphoid tissue (BALT)
* Gut-associated lymphoid tissues (GALT)
* Tonsils, adenoids, appendix, Peyer’s patches
* Pathogens are directly transferred across mucosa by
“M” cells
19.
20. THE INNATE IMMUNE RESPONSE
* Mediated (initiated) by phagocytes, NK cells and soluble
proteins
* Phagocytes
* Cells specialized in the process of phagocytosis
* Macrophages
* Reside in tissues and recruit neutrophils
* Neutrophils
* Enter infected tissues in large numbers
* Recognize common molecules of bacterial cell surface using
a few surface receptors
* Phagocytosis
* Capture, engulfment and breakdown of bacterial pathogen
21.
22. THE INNATE IMMUNE RESPONSE
* Inflammatory response enhances phagocytosis through
acute phase proteins
* Mannose-binding lectin (MBL)
* Binds to bacterial surface with particular spatial arrangement
of mannose or fucose
* C-reactive protein (CRP)
* Binds to phosphorylcholine on bacterial surface
* Complement
* Set of proteins which bind to bacterial surface
* Inflammatory response
* Accumulation of fluid and cells at infection site (swelling,
redness, heat and pain)
23.
24. THE ADAPTIVE IMMUNE
RESPONSE
* Creates millions of different B and T cells for specific
antibody-mediated and cell-mediated immunity
* Antibody-Mediated Immunity (AMI)
* Involves B lymphocytes, plasma cells and antibodies
* Humoral immunity
* Name derives from antibodies found in body fluids (humors -
old medical term)
* Cell-Mediated Immunity (CMI)
* Involves T lymphocytes, antigen-presenting cells and MHC
(major histocompatibility complex) molecules
* Cellular immunity
25. ANTIBODY-MEDIATED
(HUMORAL) IMMUNITY
* Directed against extracellular microorganisms and toxins
* B-lymphocytes (B cells)
* Differentiate into plasma cells which produce antibodies
* Function as antigen-presenting cells (APC’s)
* Classification of Antibodies (Immunoglobulins)
* Immunoglobulin M (IgM)
* Immunoglobulin G (IgG)
* Immunoglobulin A (IgA)
* Immunoglobulin D (IgD)
* Immunoglobulin E (IgE)
26. CELL-MEDIATED IMMUNITY (CMI)
* Directed against intracellular microorganisms
* Non-phagocytic cells and phagocytic cells
* T-lymphocytes (T cells)
* Differentiate into effector cells following antigen
presentation by antigen presenting cells (APC’s)
* Functional types of T cells
* Helper (CD4 T cells)
* TH1 and TH2 cells
* Cytotoxic (CD8 T cells)
* Regulatory
* CD4 and CD8 Tregs
27. THE NATURE OF ANTIGENS
* Historically named as antibody generators
* Molecule which stimulates production of and binds
specifically to an antibody
* Contemporary view distinguishes between
* Antigen
* Molecule which can bind to specific antibody but cannot elicit
adaptive immune response
* Immunogen
* Molecule which can stimulate adaptive immune response
* Best immunogens are proteins with
MW > 10,000
28. THE NATURE OF ANTIGENS
* Carbohydrates, nucleic acids and lipids are also potential
antigens / immunogens
* Hapten
* Small (low MW) molecule unable to elicit immune response
* Combines with larger carrier molecule which together
function as immunogen
* Antibody may react independently with hapten following
hapten/carrier adaptive immune response
* Example
* Penicillin G (MW of 372)
* Albumin (MW of 66,000)
29. THE NATURE OF ANTIBODIES
* Antibodies are glycoproteins
* Exist as monomers, dimers or pentamers of basic
structure
* Basic antibody structure has 4 polypeptide chains
* 2 identical light chains
* 2 identical heavy chains
* Regions of heavy and light chains
* Variable
* Constant
30.
31. THE NATURE OF ANTIBODIES
* Also referred to as
* Immune globulins / Immunoglobulins (IG)
* Immune serum globulins (ISG)
* Gamma globulins
* Contemporary immunology
* Antibody
* Secreted form of IG made by plasma cells
* Immunoglobulin
* Antigen binding molecules of B cells
* (B cell antigen receptors)
32. CLASSIFICATION OF ANTIBODIES
(IMMUNOGLOBULINS)
* Five (5) classes (isotypes)
* Immunoglobulin A (IgA)
* Immunoglobulin G (IgG)
* Immunoglobulin M (IgM)
* Immunoglobulin D (IgD)
* Immunoglobulin E (IgE)
* Based on structural differences in constant regions
of heavy chains
* Classes have specialized effector functions
33.
34. B LYMPHOCYTES AND
HUMORAL IMMUNITY
* Originate from stem cells in bone marrow
* Maturation in bone marrow followed by migration to
secondary lymphoid tissue
* Antigen exposure in secondary lymphoid tissue
* Following exposure to antigen, differentiation into plasma
cells and memory cells
* Plasma cells produce antibodies of all IG classes
35. ACTIVATION OF ANTIBODY PRODUCING
CELLS BY CLONAL SELECTION
* B lymphocytes recognize intact pathogenic
microorganisms and toxins
* B lymphocytes possess specific surface receptors for
recognition of specific antigen
* IgM and IgD
* Binding of specific antigen results in proliferation of a
clonal population of cells
* Antigen determines clonal proliferation
36.
37. ACTIVATION OF ANTIBODY
PROCDUCING CELLS BY CLONAL
SELECTION
* Proliferation of activated cells is followed by
differentiation into
* Plasma cells
* Life span of
* 4 to 5 days
* 1 to 2 months
* Produce 2,000 antibody molecules / second
* Memory cells
* Life span of years to decades
* Differentiate into plasma cells following stimulation
by same antigen
38. PRIMARY AND SECONDARY
ANTIBODY RESPONSE
* Primary Response
* Following exposure to an antigen, there is a slow
rise in IgM followed by a slow rise in IgG
* Secondary Response
* Following exposure to previously encountered
antigen, there is a rapid rise in IgG and slow or no
rise in IgM
* Memory or anamnestic response
39.
40. T LYMPHOCYTES AND CELL-MEDIATED
IMMUNITY
* Originate from stem cells in bone marrow followed by
migration to thymus gland
* Maturation takes place in thymus gland followed by
migration to secondary lymphoid tissue
* Respond to antigens on the surface of antigen presenting
cells (APC’s)
* Antigen presenting cells (APC’s)
* Macrophages
* Dendritic cells
* B lymphocytes
41. T LYMPHOCYTES AND CELL-MEDIATED
IMMUNITY
* Antigen presenting cells (APC’s)
* Ingest and process antigens then display fragments (short
peptides) on their surface in association with molecules of
major histocompatibility complex (MHC)
* Major histocompatibility (MHC) molecules
* MHC class I molecules
* Present antigens to CD8 T cells
* MHC class II molecules
* Present antigens to CD4 T cells
* T cells which encounter antigen differentiate into effector
T cells
42.
43. ROLES OF EFFECTOR T CELLS IN
IMMUNE RESPONSE
* CD8 cytotoxic T cells
* Enter bloodstream and travel to infection site
* Kill cells infected with viruses and other intracellular
microorganisms
* CD4 TH1 helper T cells
* Enter blood stream and travel to infection site
* Help activate macrophages
* CD4 TH2 helper T cells
* Work within secondary lymphoid tissues
* Help activate B cells
44.
45.
46. DISORDERS OF THE IMMUNE SYSTEM
* Hypersensitivity Reactions
* Over-reaction of adaptive immune response to harmless
antigens
* Four Types of reactions (I- IV)
* Autoimmunity
* Misdirected adaptive immune response
* Results from a loss of self-tolerance
* Three Types (II, III, IV) of reactions
* Immunodeficiencies
* Components of immune system either absent or
defective
* Genetic or acquired etiology
47. IMMUNOLOGY FOR DIAGNOSIS
OF DISEASES * Analytical methods using either antibody or antigen with
an indicator system for detecting specific
* Antibodies
* Detected using antigens or antibody
* Antigens
* Detected using antibody
* Indicator systems
* Latex particles (colored)
* Microspheres (colored) conjugated with antibody
* Enzymes conjugated to antibody
* Fluorochromes conjugated to antibody
* Nitrocellulose membranes fixed with antigen or antibody
48. METHODS IN DIAGNOSTIC
IMMUNOLOGY
* Latex agglutination (LA)
* Latex particles (dyed) coated with antigen, antibody or?
* Read visually for clumping of latex particles
* Staphyloslide (Becton Dickinson)
* Identification of Staphylococcus aureus
* Staphylococcus aureus produces
* Coagulase (bound and free)
* Protein A
* Blue latex particles coated and not coated with
* Fibrinogen
* IgG
49. METHODS IN DIAGNOSTIC
IMMUNOLOGY
* Immunochromatographic assay (ICA)
* Antibody or antigen immobilized (Test line)
* Antibody immobilized (Control line)
* Membranes
* Nitrocellulose, cellulose acetate
* Read visually for colored test and control lines
* Examples
* Group A Streptococcus (GAS) antigen
* Influenza A and B antigens
* Respiratory syncytial virus (RSV) antigen
* Rotavirus antigen
* HIV-1/2 antibody
50. PRINCIPLES OF OraQuick RAPID HIV-1/2
ICA ANTIBODY TEST
* Antigens and antibody immobilized onto nitrocellulose
membrane in T and C zones
* Test (T) Zone
* Synthetic peptides from HIV envelope region
* Control (C) Zone
* Goat anti-human IgG
* Developer solution
* Facilitates flow of specimen onto test strip
* Rehydrates protein-A gold colorimetric reagent
51. IMMUNOLOGY FOR PREVENTION
OF DISEASE
* Hepatitis B
* Pre-exposure prophylaxis
* Vaccination with hepatitis B surface antigen
(HBsAg)
* Post-exposure prophylaxis
* Administration of
* Hepatitis B Immune Globulin (HBIG) Human
* Purified IgG antibody from plasma of donors with
high titer of antibody to the hepatitis B surface
antigen (anti-HBs)
52. IMMUNOLOGY FOR TREATMENT
OF DISEASE
* Rheumatoid Arthritis
* Remicade (Infliximab)
* IgG kappa monoclonal antibody against tumor
necrosis factor – alpha (TNF-alpha)
* Breast Cancer
* Herceptin (Trastuzumab)
* IgG kappa monoclonal antibody against human
epidermal growth factor receptor 2 (HER2)