Gas chromatography is a type of chromatography that uses a mobile gas phase to transport sample components through a column containing a stationary liquid or solid phase. The separation of components is based on their distribution between the mobile gas phase and stationary phase. There are two main types - gas-solid chromatography where the stationary phase is a solid, and gas-liquid chromatography where the stationary phase is a liquid coated on an inert solid support. Common applications of gas chromatography include separation and analysis of organic and inorganic compounds, study of reaction rates and mechanisms, and determination of molecular properties.
Introduction to gas Chromatography
,Principle of gas chromatography
Instrumentation of gas Chromatography
Type of detectors of gas chromatography
Advantages of gas chromatography
Disadvantages of gas chromatography
Applications of gas chromatography
Introduction to gas Chromatography
,Principle of gas chromatography
Instrumentation of gas Chromatography
Type of detectors of gas chromatography
Advantages of gas chromatography
Disadvantages of gas chromatography
Applications of gas chromatography
Gas chromatography (GC) is a common type of chromatography used in analytical chemistry for separating and analysing compounds that can be vaporised without decomposition.
Gas chromotography (Histroy, Importance, Classification)Talal Khan
This Presentation gives the brief introduction of Gas Chromatography, its History, and its Classifications.
It also describes the mechanism through which it is operated.
Hii..
in which slide we are involving what is Gas chromatography there History, Theory & principle, Introduction, Phases, Types, Instrumentation, Application etc.
Gas chromatography (GC) is a common type of chromatography used in analytical chemistry for separating and analysing compounds that can be vaporised without decomposition.
Gas chromotography (Histroy, Importance, Classification)Talal Khan
This Presentation gives the brief introduction of Gas Chromatography, its History, and its Classifications.
It also describes the mechanism through which it is operated.
Hii..
in which slide we are involving what is Gas chromatography there History, Theory & principle, Introduction, Phases, Types, Instrumentation, Application etc.
Gas chromatography and its instrumentationArgha Sen
Gas chromatography is an unique technology which helps us in separating volatile analytes. Its is an easy and reproduciple method for detecting residual solvents found in APIs.
The GC produces a graph called a chromatogram, which shows peaks: the size of a peak indicates the amount of each component reaching the detector. The number of peaks shows different compounds present in the sample. The position of each peak shows the retention time for each compound
Gas Chromatography is an analytical techniques, used for the separation of volatile substances on the basis of their partition coefficient . In this slide you will find out different instrumentation of gas chromatography, its advantages, disadvantages and moreover its applications.
SDS-PAGE (sodium dodecyl sulfate–polyacrylamide gel electrophoresis) is a discontinuous electrophoretic system developed by Ulrich K. Laemmli which is commonly used as a method to separate proteins with molecular masses between 5 and 250 kDa.
Agarose gel electrophoresis is a method of gel electrophoresis used in biochemistry, molecular biology, genetics, and clinical chemistry to separate a mixed population of macromolecules such as DNA or proteins in a matrix of agarose, one of the two main components of agar.
Polysaccharides produced by microorganism during their growth and especially at the stationary phase of growth when there is excess of carbon source in the medium.
High molecular weight carbohydrate polymers mainly produced by bacteria and fungi.
Microbial polysaccharides are of two types:
Storage polysaccharides like glycogen, inulin etc.
Exopolysaccarides like xanthans, dextrans, levans which are secreted by the cells.
Generally, organic acids are produced commercially either by chemical synthesis or fermentation. ... All organic acids of tricarboxylic acid cycle can be produced in high yields in microbiological processes. Among fermentation processes, the production of organic acids is dominated by submerged fermentation.
Wild strains of microorganisms produce low quantities of commercially important metabolites.
Therefore we need genetic improvement to produce high quantities of metabolites/products.
Refrigeration is a technique used for preserving food in low temperatures. This procedure slow down or stop most bacteria from dividing and thereby multiplying, but do not kill them.
A patent is an exclusive right granted for an invention – a product or process that provides a new way of doing something, or that offers a new technical solution to a problem.
Rancidification is the process of complete or incomplete oxidation or hydrolysis of fats and oils when exposed to air, light, or moisture or by bacterial action, resulting in unpleasant taste and odor. Specifically, it is the hydrolysis or autoxidation of fats into short-chain aldehydes and ketones, which are objectionable in taste and odor. When these processes occur in food, undesirable odors and flavors can result.
Information contained in biological databases includes gene function, structure, localization (both cellular and chromosomal), clinical effects of mutations as well as similarities of biological sequences and structures. Biological databases can be broadly classified into sequence, structure and functional databases.
In bioinformatics and biochemistry, the FASTA format is a text-based format for representing either nucleotide sequences or amino acid (protein) sequences, in which nucleotides or amino acids are represented using single-letter codes. The format also allows for sequence names and comments to precede the sequences.
Proteins affect the sensory properties of food, i.e.,
appearance;
texture (sols, gels, foams, emulsions, extruded pieces);
colour (via browning reactions);
flavor (via browning reactions and sulphide elimination reactions, via proteolysis, and by entrapment and binding of both desirable and undesirable flavors).
The loss of native conformation brings about changes in specific properties characterizing the identity of proteins.
Bring changes in the proteins.
It makes peptide bonds more readily available for hydrolysis by proteolytic enzymes.
Protein solubility decreased (hydrophobic groups exposed out).
Biological properties (catalytic, hormonal) are lost.
Viscosity and optical rotation increases.
It is a comprehensive, authoritative and timely knowledgebase of human genes and genetic disorders compiled to support human genetics research and education and the practice of clinical genetics.
One of the best websites for detailed and updated information of genetic diseases.
Set up in 1995 by the National Centre for Biotechnology Information (NCBI).
Antigen
Antigen is a substance which binds specifically with the products (antibodies, T-cells) of the immune system.
Its ability to bind with antibodies is called antigenicity.
Immunogen
It is a substance which produces an immune response as well as binds to its products.
So, immunogen is an antigen as well but antigen need not be immunogen.
The property of producing an immune response is called immunogenicity.
Archive of experimentally determined 3D structures of biological macromolecules.
Established in 1971, by Research Collaboratory for Structural Bioinformatics (RCSB), Brookhaven National Laboratories, USA.
Archive contain atomic coordinates, bibliographic citations, primary and secondary structure information, crystallographic structure factors, NMR experimental data.
The French Revolution, which began in 1789, was a period of radical social and political upheaval in France. It marked the decline of absolute monarchies, the rise of secular and democratic republics, and the eventual rise of Napoleon Bonaparte. This revolutionary period is crucial in understanding the transition from feudalism to modernity in Europe.
For more information, visit-www.vavaclasses.com
How to Create Map Views in the Odoo 17 ERPCeline George
The map views are useful for providing a geographical representation of data. They allow users to visualize and analyze the data in a more intuitive manner.
The Indian economy is classified into different sectors to simplify the analysis and understanding of economic activities. For Class 10, it's essential to grasp the sectors of the Indian economy, understand their characteristics, and recognize their importance. This guide will provide detailed notes on the Sectors of the Indian Economy Class 10, using specific long-tail keywords to enhance comprehension.
For more information, visit-www.vavaclasses.com
This is a presentation by Dada Robert in a Your Skill Boost masterclass organised by the Excellence Foundation for South Sudan (EFSS) on Saturday, the 25th and Sunday, the 26th of May 2024.
He discussed the concept of quality improvement, emphasizing its applicability to various aspects of life, including personal, project, and program improvements. He defined quality as doing the right thing at the right time in the right way to achieve the best possible results and discussed the concept of the "gap" between what we know and what we do, and how this gap represents the areas we need to improve. He explained the scientific approach to quality improvement, which involves systematic performance analysis, testing and learning, and implementing change ideas. He also highlighted the importance of client focus and a team approach to quality improvement.
Unit 8 - Information and Communication Technology (Paper I).pdfThiyagu K
This slides describes the basic concepts of ICT, basics of Email, Emerging Technology and Digital Initiatives in Education. This presentations aligns with the UGC Paper I syllabus.
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This presentation provides a briefing on how to upload submissions and documents in Google Classroom. It was prepared as part of an orientation for new Sainik School in-service teacher trainees. As a training officer, my goal is to ensure that you are comfortable and proficient with this essential tool for managing assignments and fostering student engagement.
Synthetic Fiber Construction in lab .pptxPavel ( NSTU)
Synthetic fiber production is a fascinating and complex field that blends chemistry, engineering, and environmental science. By understanding these aspects, students can gain a comprehensive view of synthetic fiber production, its impact on society and the environment, and the potential for future innovations. Synthetic fibers play a crucial role in modern society, impacting various aspects of daily life, industry, and the environment. ynthetic fibers are integral to modern life, offering a range of benefits from cost-effectiveness and versatility to innovative applications and performance characteristics. While they pose environmental challenges, ongoing research and development aim to create more sustainable and eco-friendly alternatives. Understanding the importance of synthetic fibers helps in appreciating their role in the economy, industry, and daily life, while also emphasizing the need for sustainable practices and innovation.
The Roman Empire A Historical Colossus.pdfkaushalkr1407
The Roman Empire, a vast and enduring power, stands as one of history's most remarkable civilizations, leaving an indelible imprint on the world. It emerged from the Roman Republic, transitioning into an imperial powerhouse under the leadership of Augustus Caesar in 27 BCE. This transformation marked the beginning of an era defined by unprecedented territorial expansion, architectural marvels, and profound cultural influence.
The empire's roots lie in the city of Rome, founded, according to legend, by Romulus in 753 BCE. Over centuries, Rome evolved from a small settlement to a formidable republic, characterized by a complex political system with elected officials and checks on power. However, internal strife, class conflicts, and military ambitions paved the way for the end of the Republic. Julius Caesar’s dictatorship and subsequent assassination in 44 BCE created a power vacuum, leading to a civil war. Octavian, later Augustus, emerged victorious, heralding the Roman Empire’s birth.
Under Augustus, the empire experienced the Pax Romana, a 200-year period of relative peace and stability. Augustus reformed the military, established efficient administrative systems, and initiated grand construction projects. The empire's borders expanded, encompassing territories from Britain to Egypt and from Spain to the Euphrates. Roman legions, renowned for their discipline and engineering prowess, secured and maintained these vast territories, building roads, fortifications, and cities that facilitated control and integration.
The Roman Empire’s society was hierarchical, with a rigid class system. At the top were the patricians, wealthy elites who held significant political power. Below them were the plebeians, free citizens with limited political influence, and the vast numbers of slaves who formed the backbone of the economy. The family unit was central, governed by the paterfamilias, the male head who held absolute authority.
Culturally, the Romans were eclectic, absorbing and adapting elements from the civilizations they encountered, particularly the Greeks. Roman art, literature, and philosophy reflected this synthesis, creating a rich cultural tapestry. Latin, the Roman language, became the lingua franca of the Western world, influencing numerous modern languages.
Roman architecture and engineering achievements were monumental. They perfected the arch, vault, and dome, constructing enduring structures like the Colosseum, Pantheon, and aqueducts. These engineering marvels not only showcased Roman ingenuity but also served practical purposes, from public entertainment to water supply.
We all have good and bad thoughts from time to time and situation to situation. We are bombarded daily with spiraling thoughts(both negative and positive) creating all-consuming feel , making us difficult to manage with associated suffering. Good thoughts are like our Mob Signal (Positive thought) amidst noise(negative thought) in the atmosphere. Negative thoughts like noise outweigh positive thoughts. These thoughts often create unwanted confusion, trouble, stress and frustration in our mind as well as chaos in our physical world. Negative thoughts are also known as “distorted thinking”.
2. GAS CHROMATOGRAPHY
It is a type of Column chromatography. Stationary phase can be in solid or liquid form. If
stationary phase is in solid form then the technique will be called as Gas-Solid
Chromatography, If the stationary phase is in liquid phase, then the technique will be called as
Gas-Liquid Chromatography. Mobile phase will always be in gas phase.
Stationary Phase Mobile Phase
In GLC, Liquid phase dispersed on the solid surface of column and interacts with components
to be separated on the basis of their partition coefficient.
Solid Liquid Gas
Gas Solid
Chromatography
(GSC)
Gas Liquid
Chromatography
(GLC)
Carrier Gas
(named because it carries
sample components with itself)
3. Therefore, separation of components in GC is done on the basis of
their Distribution/Retardation Factor
Stationary/Liquid Phase (SP) Mobile/Gas Phase (MP)
Sample Retard More
distributed more in MP
Sample Retard Less
Sample (Volatile)
Distributed
between
distributed more in SP
Samples to be separated must be in volatile form and they get separated by their
distribution in the stationary or mobile phase. Samples distributed more in
stationary phase, remain in the column for longer period. Samples which distributed
more in mobile phase, travel along with mobile phase and spend less time in the
column. This simple principle cause their separation.
4. Chromatogram
Area under peaks tells us about
concentration of given component.
Separated Components
Elute from Column
Detector
Electrical Signal
in the form of peaks
Chromatogram
Retention Volume: Volume of gas traversed through column between the time that the sample
was applied and the time at which the particular component emerged out from the column.
Retention Volume
Sample In
Time
Sample Out
Time
Gas
Chromatogram is the graph that produced when separated samples elute from the column and
reach the detector. This graph is in the form of electrical signals in the form of peaks.
5. Carrier Gas (Mobile Phase)
Characteristics of carrier gas:
• Carrier gas must be Inert & Pure.
e.g. Nitrogen, Argon, Helium, Hydrogen, Carbon dioxide
Purity
• Gas must be free from contamination
Mostly contamination: Water vapours, which are removed by cold molecular sieve.
High Density Low Density
Pros: Better Separation
Cons: Long separation Time
Pros: Short separation Time
Cons: Poor Separation
Mobile phase can be of high density or low density which has it’s own pros and cons.
Mostly high density carrier gas is used as mobile phase for better separation.
6. Column
2 TYPES
Packed Column
Open Tubular/
Capillary Column
Wall Coated Open
Tubular Column
Support Coated Open
Tubular Column
Stainless steel, Copper, Glass tubing Provides Open unrestricted path for carrier gas in column
7. Solid Support
Properties:
1. Chemically Inert
2. Mechanically strong
3. Thermally stable
4. High surface area
Most Commonly used: Diatomaceous earth and Teflon
Deactivation of diatomaceous earth is required prior to use. Because surface of
diatomaceous earth is polar and it contains mineral impurities.
Sample components being polar, attach to diatomaceous earth surface which leads to long
retention time in column.
Mineral impurities acts as adsorption sites, where sample components adsorb which too
leads to long retention time in column.
Deactivation is done by 2 treatments:
1. Acid washing (remove mineral impurities)
2. Treatment with hexamethyl-disilazane (HMDS) make the surface inert, to polar
sample components.
Note: If sample is Non-polar/less polar: 1st treatment is enough.
If sample is Polar: Both treatments are required.
8. Liquid Phase (Stationary Phase)
Properties:
1. Chemically Inert
2. Thermally stable
3. Non-volatile
4. Good partition coefficient values for components to be separated
Liquid phase + low boiling solvent
(pentane, acetone)
Solvent evaporation
Packed column (In steps) Open Tubular column (In
steps)
Pouring the liquid phase by
straightening the column
Dilute liquid phase is forced
through column at a very slow
rate
Gently shaken & Tapping to
ensure even packing/ 5 psi
pressure is used
Excess solution is evaporated by
passing a hot carrier gas
Then, Both ends plugged with
glass wool
This results in thin layer of liquid
phase
Then, Column bend or coiled to
fit in oven.
Slow heating
Column FillingLiquid Phase Preparation
9. Sample Preparation and Introduction
Sample
Non-Polar/
Less Polar
Polar
No Pretreatment
Possess Polar functional Groups
-OH, -COOH, -NH2
Pretreatment is needed
If No Pretreatment, Sample retain in
Column for longer duration
Poor separation and Peak Tailing
Pretreatment by Derivatization:
1. Methylation
2. Silanization
3. Trifluoromethylsilanization
Increase volatility and distribution coefficient.
10. Introduction of Sample
After Pretreatment, Vaporization is done.
Sample must be introduced in vapour form. If sample is not already a vapour,
sample is heated to vaporize.
Liquid sample
Ether, Heptane, Methanol
Injected from a Syringe
into heated chamber
preceding the column
Dissolved in
11. Gas Solid Chromatography (GSC)
Stationary Phase (SP) Mobile Phase (MP)
Solid Gas
Not Inert
Therefore, Interact with sample components
Molecule/Component Separation
Adsorption-Desorption Property
Solid SP
Gas MP
Adsorption
Desorption
Sample Components
Out
Inert Column Surface
Inert Column Surface
13. DETECTORS
Temperature of Detector is maintained
1. Flame ionization detector
2. Electron capture detector
3. Thermionic emission detector
High Not Too High
To prevent condensation
of sample components
Cause decomposition of
sample components
14. 1. Flame ionization detector
Most widely used.
Hydrogen gas (carrier) used is burnt into a colorless flame.
2 electrodes = Polarizing electrode
Collector electrode
Flame change colour as the sample comes out of the column into the flame.
Sample components become ionized in the flame and give rise to current between the electrodes.
15. 2. Electron capture detector
This detector has the radioactive source, which ionize the carrier gas coming out
of the column
electrons
current
Sample component comes out of column
Capture electrons
Drop in current
Measured & Recorded
16. APPLICATIONS
1. Study of polymers, Lewis acid-base properties,
liquid crystals.
2. To study reaction rates and mechanism.
3. To study isotope labeled organic and inorganic
compounds
4. To study molecular properties such as vapor
pressure, heat of vaporization, molecular weight,
molecular geometry, bond angle deformation,
ionization potential and electron affinity.