This document discusses enzymes, including their history, classification, and functions. It begins by explaining why enzymes are essential for life, as they allow chemical reactions to occur efficiently. The first discovery of enzymes was in the 1700s during meat digestion research. Key milestones in enzyme research are highlighted, such as the discovery that enzymes are proteins. Enzymes are classified into six major categories based on their catalytic activity. The categories are oxidoreductases, transferases, hydrolases, lyases, isomerases, and ligases. Each category is defined by the type of reaction it catalyzes. The document emphasizes that enzymes are essential catalysts that allow life to be maintained by speeding up metabolic reactions.
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A comprehensive presentation on Factors affecting enzyme activity & Kinetics of Enzymes for MBBS ,BDS, B Pharm & Biotechnology students to facilitate self- study.
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Enzyme - Introduction, Biocatalysts, Definition of enzymes, Types of enzymes, classification of enzyme, Nomenclature of enzymes, EC number, Types of enzymes with examples, and reaction.
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Tom Selleck, an enduring figure in Hollywood. has captivated audiences for decades with his rugged charm, iconic moustache. and memorable roles in television and film. From his breakout role as Thomas Magnum in Magnum P.I. to his current portrayal of Frank Reagan in Blue Bloods. Selleck's career has spanned over 50 years. But beyond his professional achievements. fans have often been curious about Tom Selleck Health. especially as he has aged in the public eye.
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Introduction
Many have been interested in Tom Selleck health. not only because of his enduring presence on screen but also because of the challenges. and lifestyle choices he has faced and made over the years. This article delves into the various aspects of Tom Selleck health. exploring his fitness regimen, diet, mental health. and the challenges he has encountered as he ages. We'll look at how he maintains his well-being. the health issues he has faced, and his approach to ageing .
Early Life and Career
Childhood and Athletic Beginnings
Tom Selleck was born on January 29, 1945, in Detroit, Michigan, and grew up in Sherman Oaks, California. From an early age, he was involved in sports, particularly basketball. which played a significant role in his physical development. His athletic pursuits continued into college. where he attended the University of Southern California (USC) on a basketball scholarship. This early involvement in sports laid a strong foundation for his physical health and disciplined lifestyle.
Transition to Acting
Selleck's transition from an athlete to an actor came with its physical demands. His first significant role in "Magnum P.I." required him to perform various stunts and maintain a fit appearance. This role, which he played from 1980 to 1988. necessitated a rigorous fitness routine to meet the show's demands. setting the stage for his long-term commitment to health and wellness.
Fitness Regimen
Workout Routine
Tom Selleck health and fitness regimen has evolved. adapting to his changing roles and age. During his "Magnum, P.I." days. Selleck's workouts were intense and focused on building and maintaining muscle mass. His routine included weightlifting, cardiovascular exercises. and specific training for the stunts he performed on the show.
Selleck adjusted his fitness routine as he aged to suit his body's needs. Today, his workouts focus on maintaining flexibility, strength, and cardiovascular health. He incorporates low-impact exercises such as swimming, walking, and light weightlifting. This balanced approach helps him stay fit without putting undue strain on his joints and muscles.
Importance of Flexibility and Mobility
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Ethanol (CH3CH2OH), or beverage alcohol, is a two-carbon alcohol
that is rapidly distributed in the body and brain. Ethanol alters many
neurochemical systems and has rewarding and addictive properties. It
is the oldest recreational drug and likely contributes to more morbidity,
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comorbidities, fetal alcohol spectrum disorders, genetic risk factors, and
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3. What shall we learn?
• Why should we learn about enzymes?
• Why medicos should know about them?
• Who thought about them first?- History
• How do we define them?
• How do we name them?- The
classification
4. Why enzymes???
Two Fundamental Conditions for Life :
(1) able to self-replicate
(2) able to catalyze chemical reaction
efficiently and selectively
Example : a bag of sugar can be stored for
years, but it releases its chemical energy in
seconds in human body …… “ catalysis”
Life depends on well-orchestrated series
of chemical reactions : E. coli has 4288
proteins, 2656 of which are characterized,
and 64% (1701) of the characterized ones
code enzymes
5. Chemical reactions are far slow to
maintain life
Living system has designed catalysts
to fasten the specific reactions
Breakdown of nutrients to supply
energy
Assembly of building blocks
Diagnostic importance- diagnosis &
prognosis
6. Most of the history of Biochemistry is
enzyme research
• 1700- Meat digestion by stomach
• 1800- Conversion of starch to glucose by saliva
• 1850 Louis Pasteur .. Fermentation ( sugar →
alcohol)- said ferments are inseparable- Theory of
‘Vitalism’
• 1897 Edward Buchner …… yeast extract → enzyme
‘zymase’ (Sucrose → Alcohol)
• Willey Kunhe.. Coined ‘Enzyme’
• 1926 James Sumner … Urease crystallization →
protein- theory of vitalism rejected
• J.B.S. Haldane …… enzyme-substrate interaction
7.
8.
9.
10. Milestones in enzyme study
• Koshland (1958)- “Induced fit” model was
proposed,
• Monod (1965)- “Allosteric model” of enzyme was
proposed,
• 1969- the first chemical synthesis of an enzyme
was reported, proving an enzyme is a protein
• Mechanisms of thousands enzymes have been
studied by X-ray crystallography and NMR
• DNA recombinant methods were used to
overproduce enzymes and to pinpoint the
important amino acids
• 2004, the first computer designed enzyme was
reported
12. Definition
Enzymes are “Biocatalysts”, synthesized by living
cells and highly specific in their action.
They are:
Mostly proteins (Exception- Ribozymes)
Heat labile
Soluble in water
Colloidal
Precipitated by precipitation reaction
Contain 16% weight as nitrogen
13. Classification & Nomenclature
A. Recommended name
B. Systematic name
IUBMB
Unambiguous & Informative
But Cumbersome
6 major classes
Class.Subclass.sub-subclass.substrate
14.
15. OTHLIL
Oxidoreductases→ Transfer of H, O or e-
Transferases → Transfer of gr other than H
Hydrolases → Cleave bond & add H2O
Lyases → Cleave bond without adding H2O
Isomerases → Intramolecular transfers
Ligases → ATP dependent condensation of
2 molecules
16. 1. Oxidoreductases
AH2 + B →A + BH2
Alcohol+ NAD+ ADH Aldehyde + NADH +H+
• Dehydrogenases (hydride transfer)
• Oxidases (electron transfer to molecular
oxygen)
• Oxygenases (oxygen transfer from molecular
oxygen)
• Peroxidases (electron transfer to peroxide).
17. 2. Transferases
A-R + B → A + B-R
• transfer of an atom or group of atoms (e.g.
acyl-, alkyl- and glycosyl- ), between two
molecules, but excluding such transfers as
are classified in the other groups (e.g.
Oxidoreductases and Hydrolases).
• Ex- Aminotransferases, all kinases,
transmethylases
• Hexose + ATP Hexokinase Hexose-6-P +
ADP
18. 3. Hydrolases
Cleavage of ester, ether, peptide or
glycosidic bond by addition of H2O
Ach + H2O acetylcholine esterase Choline + Acetate
Ex: All digestive enzymes, lipase, pepsin,
Trypsin, ALP, Urease
19. 4. Lyases
Cleave bond without addition of H2O
Fructose-1,6-BP Aldolase Glyceraldehyde-3-P
+ Dihydroxyacetone P
Ex: Fumarase, Histidase, HMG CoA lyase
20. 5. Isomerases
Can produce optical, geometrical or
positional isomers of substrates
Gly-3-P Triose P isomerase DHAP
Ex: Racemase, Epimerase
21. 6. Ligases (synthetases)
ATP dependent condensation of two
molecules
Acetyl CoA + CO2 + ATP Acetyl CoA Carboxylase
Malonyl CoA + ADP + Pi
Synthases and synthetases are different !!!
Ex: Glycogen synthase, ALA synthase
22. We are what we repeatedly do;
Excellence is then not an act but a
23. For more Medical Biochemistry ppt please
visit www.vpacharya.com
Editor's Notes
Conversion of sugar into CO2 and H2O is highly exergonic and thermodynamically favorable, yet so slow. But in human body it catabolises immediately. Reactions should take place in proper time scale too.