CLINICAL CONSIDERATIONSNoncommunicating (obstructive) hydrocephalus occurs more frequentlyCSF of ventricles unable to reach subarachnoid spaceProduction of CSF continuesGyri are flattened against inside of skullIf skull is still pliable head may enlarge
This presentation is focused on diagnostic utility of Red blood cell indices which will be very useful for undergraduate and postgraduate of medical field.
An immature red blood cell without a nucleus, having a granular or reticulated appearance when suitably stained.
Reticulocytes are the immature RBC that contain nucleus.
They are originally seen at the site of their formation i.e. bone marrow. They take 2-3 (lays for maturation only about 1-2% of circulating RBCs are Reticulocytes.
Analysis of CSF, Gucose, Protien, Albumin, Globulin, Function of CSF, Secretion of CSF, Absorption of CSF, Bacterial Infection, Clinical Significance, Normal Composition of CSF, Microscopic examination of CSF, COOMASSIE BRILLIANT BLUE(CBB), REVERSE BIURET METHOD
It is about interpretation of information delivered as results of pleural fluid exams, including physical characteristics, differentiation between transudates and exudates, cell counts, culture and cytology.
This presentation is focused on diagnostic utility of Red blood cell indices which will be very useful for undergraduate and postgraduate of medical field.
An immature red blood cell without a nucleus, having a granular or reticulated appearance when suitably stained.
Reticulocytes are the immature RBC that contain nucleus.
They are originally seen at the site of their formation i.e. bone marrow. They take 2-3 (lays for maturation only about 1-2% of circulating RBCs are Reticulocytes.
Analysis of CSF, Gucose, Protien, Albumin, Globulin, Function of CSF, Secretion of CSF, Absorption of CSF, Bacterial Infection, Clinical Significance, Normal Composition of CSF, Microscopic examination of CSF, COOMASSIE BRILLIANT BLUE(CBB), REVERSE BIURET METHOD
It is about interpretation of information delivered as results of pleural fluid exams, including physical characteristics, differentiation between transudates and exudates, cell counts, culture and cytology.
astigmatism
AstigmatismAstigmatism Walter Huang, ODWalter Huang, OD Yuanpei UniversityYuanpei University Department of OptometryDepartment of Optometry
2. DefinitionDefinition When parallel rays of light enter the eyeWhen parallel rays of light enter the eye ((with accommodation relaxedwith accommodation relaxed) and do) and do notnot come to a single point focus on or nearcome to a single point focus on or near the retinathe retina
3. OpticsOptics Power in thePower in the horizontalhorizontal plane projects aplane projects a verticalvertical focal line imagefocal line image Power in thePower in the verticalvertical plane projects aplane projects a horizontalhorizontal focal line imagefocal line image
4. OpticsOptics Refraction of light taking place at a toricRefraction of light taking place at a toric surface: the conoid of Sturmsurface: the conoid of Sturm
TONOMETRY • Tonometry is the procedure performed to determine the intraocular pressure (IOP).
3. CLASSIFICATION TONOMETRY DIRECT INDIRECT Indentation Applanation Manometer
4. APPLANATION Contact Non-contact Goldmann Perkins Air-puff Pulse air
5. INDENTATION TONOMETER • It is based on fundamental fact that plunger will indent a soft eye more than hard eye. • The indentation tonometer in current use is that of Schiotz . • It was devised in 1905 and continued to refine it through 1927.
6. PROCEDURE • Patient should be anaesthetising with 4% lignocaine or 0.5% proparacaine. • with the patient in supine position, looking up at a fixation target while examiners separates the lids and lower the tonometer plate to rest on the cornea so that plunger is free to move. •
. Introduction Biomicroscope derives its name from the fact that it enables the practitioner to observe the living tissue of eye under magnification. It not only provides magnified view of every part of eye but also allows quantitative measurements and photography of every part for documentation.
3. • The lamp facilitates an examination which looks at anterior segment, or frontal structures, of the human eye, which includes the –Eyelid –Cornea –Sclera –Conjunctiva –Iris –Aqueous –Natural crystalline lens and –Anterior vitreous.
4. Important historical landmarks De Wecker 1863 devised a portable ophthalmomicroscope . Albert and Greenough 1891,developed a binocular microscope which provided stereoscopic view. Gullstrand ,1911 introduced the illumination system which had for the first time a slit diapharm in it Therefore Gullstrand is credited with the invention of slit lamp.
1. Introduction Gross anatomy Layers Blood supply, drainage and nerve supply
2. INTRODUCTION • Sclera forms posterior 5/6th of external tunic , connective tissue coat of eyeball. • it continues with duramater and cornea • Its whole surface covered by tenon’s capsule • Anteriorly covered by- bulbar conjunctiva • Inner surface lies in contact with choroid • With a potential suprachoroidal space in between
3. Equa THICKNESS OF SCLERA
4. • Thickness varies with individual, with age • Thinner- children, elder, F> M • Thickest posteriorly • Gradually becomes thinner when traced anteriorly • Thin at insertion of extraocular muscle
The pupil is an opening located in the center of the iris that allows light to enter the retina. • Its function is to control the amount of light entering the eye and it does this via contraction (miosis) and dilation (mydriasis) under the influence of the autonomic nervous system
3. • The iris is a contractile structure, consisting mainly of smooth muscle, surrounding the pupil. Light enters the eye through the pupil, and the iris regulates the amount of light by controlling the size of the pupil.
4. The iris contains two groups of smooth muscles: a circular group called the sphincter pupillae. and a radial group called the dilator pupillae.
5. Parasympathetic pathway • First Order – Retina to Pretectal Nucleus in B/S (at level of Superior colliculus) Second Order – Pretectal nucleus to E/W nucleus (bilateral innervation!) Third Order – E/W nucleus to Ciliary Ganglion Fourth Order – Ciliary Ganglion to Sphincter pupillae (via short ciliary nerves) • • •
The tear film constitutes Three layers :- An outermost lipid (oily) layer An aqueous (watery) layer that makes up 90% of the tear film volume; and A mucin layer that coats the corneal surface.
3. To form smooth optical surface on cornea. To keep the surface of cornea & conjunctiva moist It serve as lubricant It transfer oxygen Provide antibacterial action Wash debris out It provides a pathway for WBC in case of injury
4. Functions of lipid layer Retards evaporation of tear film Prevents the overflow of tears
5. Function of Aqueous Layer Flushes, buffers and lubricates the corneal surface Delivers oxygen and other nutrients to the corneal surface Wash out debris Delivers antibacterial enzymes and antibodies such as lysozyme.
6. Functions of Mucin Layer Spreads tears over corneal surface. Protects the cornea against foreign substances . Makes corneal surface smooth by filling in surface irregularities
Introduction Transparent,avascular,watch-glass like structure. Forms 1/6th part of outer fibrous coat (Sclera) It is the major refracting surface of the eye
3. Dimensions + Avg horizontal dia =11.75 mm (ant surface) + Avg vertical dia = 11 mm (ant surface ) + Avg dia (post surface)= 11.5 mm + Thickness(centre) =0.52mm + Thickness(peripheral) = 0.67mm + Radius of curvature (ant surface) = 7.8mm + Radius of curvature (post surface)= 6.5mm + Refractive power (ant surface) = +48D + Refractive power(post surface)= - 5D + R.I = 1.376
4. Histology + Epithelium + Bowman’s membrane + Stroma + Dua’s layer + Descemet’s membrane + Endothelium
5. Epithelium + Made up of stratified squamous epithelium + Thickness - 50-90 um + 5-6 layers of cells + Regenerative, entire epithelial layer is replaced every 6-8 days + Made up of 3 types of cells - basal,wing, flattened cells + Cells are attached by to each other by means of desmosomes & maculi occludents
6. Bowman’s membrane + Acellular,Non regenerative + Made up of condensed collagen fibrils. + Thickness - 12um + Resistant to infection & injury.
LIMBUS… • The limbus forms the border between the transparent cornea and opaque sclera, contains the pathways of aqueous humour outflow, and is the site of surgical incisions for cataract and glaucoma
2. Anatomical Limbus: Circumcorneal transitional zone of the conjunctivocorneal & corneoscleral junction Conjunctivo-corneal junction: • Bulbar conjunctiva is firmly adherent to underlying structures • Substantia propria of the conjunctiva stops here but its epithelium continues with that of the cornea. Sclero-corneal junction: • Transparent corneal lamellae become continuous • With the oblique, circular and opaque fibres of sclera
3. CONTINUE…. • In the area near limbus, the conjunctiva, tenon’s capsule & the episcleral tissue are fused into a dense tissue which is strongly adherent to corneo scleral junction.It is preferred site for obtaining a firm hold of the eyeball during ocular surgery. • The limbus is a common site for the occurrence of corneal epithelial neoplasm. • The Limbus contains radially oriented fibrovascular ridge known as the palisades of Vogt that may harbour a stem cell population. The palisades of Vogt are more common in the superior and inferior quadrants around the eye
Diagnosis, Management, and Surgery by Adam J. Cohen, Michael Mercandetti & Brian G. Brazzo. The dry eye , a practical approach by Sudi Patel & Kenny J Blades. Jack J Kanski’s clinical ophthalmology Clinical Anatomy of the Eye by Richard S. Snell & Michael A. Lemp.
3. It is concerned with the tear formation & transport. Lacrimal passage includes : Lacrimal gland Conjunctival sac Lacrimal puncta Lacrimal canaliculi Lacrimal sac Nasolacrimal duct
4. The following components of the lacrimal apparatus are discussed : Embryology Osteology Secretory system Excretory system Physiology
5. Ectodermal origin Solid epithelial buds(first 2 months) Supero
Extraocular musles(EOM) They are six in number Four recti: Superior rectus Inferior rectus Medial rectus Lateral rectus Two oblique muscles: Superior oblique Inferior oblique
3. SUPERIOR RECTUS MUSCLE . Origin Superior part of common annular tendon of Zinn Course Passes anterolaterally beneath the levator At 23 degrees with the globe ‘s AP axis Pierces Tenon s capsule Insertion into sclera by flat tendinous 10 mm broad insertion 7.7 mm behind sclero-corneal junction. 42 mm long 9 mm wide
4. Nerve supply Sup division of 3rd N Blood Supply Lateral Muscular br. of Ophthalmic A APPLIED: SR loosely bound to LPS muscle. • During SR resection- eyelid may be pulled forward narr owing palpebral fissure • In hypotropia pseudoptosis may be present Origin of SR and MR are closely attached to the dural sheat h of the optic nerve pain during upward & inward movements of the globe in RETROBULBAR NEURITIS
Diseases of sclera
2. anatomy • Sclera posterior 5/6th opaque part of the external fibrous tunic of the eyeball.
3. • outer surface }covered by Tenon's capsule. • anterior part } covered by bulbar conjunctiva.
4. Its inner surface lies in contact with choroid with a potential suprachoroidal space in between
5. Thickness of sclera. • thinner }children and in females Sclera • thickest} posteriorly (1mm) • gradually becomes thin when traced anteriorly. • thinnest } insertion of extraocular muscles (0.3 mm). • Lamina cribrosa is a sieve-like sclera from which fibres of optic nerve pass.
6. Apertures of sclera • Anterior • Anterior ciliary vessels • Middle • four vortex veins (vena verticosae) • Posterior • Optic nerve • Long & short ciliary nerves
7. Layers of sclera sclera episclera Sclera proper Lamina fusca thin, dense vascularised layer of connective tissue fibroblasts, macrophages and lymphocytes avascular structure dense bundles of collagen fibres. innermost blends with suprachoroidal and supraciliary laminae of the uveal tract. brownish in colour presence of pigmented cells.
Main physiologic function of cornea is to act as a major refracting medium, so that a clear retinal image is formed. • Normal corneal transparency is result of • 1.anatomical factor such as uniform and regular arrangement of corneal epithelium, peculiar arrangement of corneal lamella and corneal vascularity 2.Physiological factor [ie] relative state of corneal dehydration.
3. • Therefore, any process which upsets the anatomy or physiology of cornea will cause LOSS OF TRANSPARENCY to some degree.
4. FACTORS AFFECTING CORNEAL TRANSPARENCY • CORNEAL EPITHELIUM &TEAR FLIM • ARRANGEMENT OF STROMAL LAMELLA • CORNEAL VASCULARIZATION • CORNEAL HYDRATION • CELLULAR FACTORS AFFECTING TRANSPARENCY
CONJUNCTIVA: ANATOMY , PHYSIOLOGY, SYMPTOMATOLOGY AND CLASSIFICATION Pranay Shinde DNB Resident Deen Dayal Upadhyay Hospital,New Delhi
2. ANATOMY It is the mucous membrane covering the under surface of the lids and anterior part of the eyeball upto the cornea.
3. Parts of conjunctiva • Palpebral; covering the lids—firmly adherent. • Forniceal; covering the fornices—loose—thrown into folds. • Bulbar; covering the eyeball—loosely attached except at limbus.
4. Palpebral conjunctiva • Subtarsal sulcus 2mm from posterior edge of the lid margin. • Richly vascular. • Extremely thin. • Strongly bound to the tarsal plate.
5. Palpebral conjunctiva is subdivided into three parts: 1)Marginal 2)Tarsal 3)Orbital
6. Conjunctival fornices • Transitional region between palpebral and bulbar conjunctivae. • Superior fornix 10 mm from limbus. • Inferior fornix 8 mm from limbus. • Lateral fornix 14mm from limbus. • Medially absent. • Ducts of lacrimal glands open into lateral part of superior fornix.
q Colour Vision Deficiency Presented by : Optometrist (intern) Asma Al-Jroudi Saudi Arabia, Riyadh, King Abdulaziz University Hospital 30 Dec 14
2. • What Is Color Vision Deficiency? • Causes Of Color Vision Deficiency • Types Of Color Vision Deficiency • Tretments Of Color Vision Deficiency • Ishihara’s Test • Conclousion
3. What is Colour Blindness? • Color blindness, or color vision deficiency, is the inability or decreased ability to see color, or perceive color differences, under normal lighting conditions. •This condition results from an absence of color- sensitive pigment in the cone cells of the retina, the nerve layer at the back of the eye.
4. What is Colour Blindness? • Cones are the coulored light receptors in back of the eye: Red light receptors, Blue light receptors and Green light receptors. • Colour blindness occurs when one or more of the cone types are defected.
5. Causes of Color Blindness • Genetic: Many more men are affected than women. • Acquired : Chronic illness, Accidents, Medications and Age.
ANATOMY & PHYSIOLOGY Lecturer: Tatyana V. Ryazantseva
2. Outer eye: Eyelids The eyelids fulfill two main functions: protection of the eyeball secretion, distribution and drainage of tears
3. Lid movement The levator extends from an attachment at the orbital apex to attachments at the tarsal plate and skin. ● The lids are securely attached at either end to the bony orbital margin by the medial and lateral palpebral ligaments. Trauma to the medial ligament causes the lid to flop forward and laterally, impairing function and cosmesis.
4. Innervation - Sensory innervation is from the trigeminal (fifth) cranial nerve, via the ophthalmic division (upper lid) and maxillary division (lower lid). - The orbicularis oculi is innervated by the facial (seventh) nerve. - The levator muscle in the upper lid is supplied by the oculomotor (third) nerve.
5. Blood supply and lymphatics The eyelids are supplied by an extensive network of blood vessels which form an anastomosis between branches derived from the external carotid artery via the face and from the internal carotid artery via the orbit.
6. Blood supply and lymphatics Lymphatic fluid drains into the preauricular and submandibular nodes. Preauricular lymphadenopathy is a useful sign of infective eyelid swelling (especially viral).
Anatomy and Physiology of Aqueous Humor Sumit Singh Maharjan
2. Anatomy
3. Angle of anterior chamber
4. Angle of the Anterior chamber
5. Gonioscopic grading of Angle
6. Aqueous Outflow system
7. Trabecular meshwork
8. Functions of Aqueous Humor • Maintenance of Intraocular pressure • Metabolic role cornea lens vitreous and retina • Optical function • Clearing function
9. Physicochemical properties • volume: 0.31ml (0.25ml in Ant. Chamber and 0.06 in post chamber) • Refractive index: 1.336 • Density: slightly greater than water, its viscocity is 1.025-1.040 • Osmotic pressure: slightly hyperosmotic to plasma by 3-5mosm/l • PH: 7.2 • Rate of formation: 2-2.5microliter/min
10. Biochemical composition • Water: 99.9% • Proteins: 5-16mg/100ml • Amino acids: aqueous/plasma concentration varies from 0.08-3.14 • Non colloidal constituents: conc. of ascorbate, pyruvate, lactate in higher am
Vitreous humour
1. Vitreous Humour
2. General features Vitreous humour is an inert ,transparent , colourless, jellylike, hydrophilic gel that serves the optical functions and also acts as important supporting structures for the eyeball. The vitreous cavity is bounded by anteriorly by the lens and ciliary body and posteriorly by the retina Its weighs nearly 4g Vitreous is an extacellular material composed of approximately 99 per cent water
3. Structure The vitreous body is the largest and simplest connective tissue present as a single piece in the human body Divided into three parts- 1. The hyaloid layer or membrane 2. The cortical vitreous and 3. The medullary vitreous
Vitamins all
1. Vitamins. Definition - Organic compound required in small amounts. Vitamin A Vitamin B1, B2, B3, B5, B6, B7, B9, B12 Vitamin D Vitamin E Vitamin K A few wordsabout each.
2. Sourcesin diet - Many plants(photoreceptors), also meat, especially liver. Fat soluble, so you can get too much, or too littleif absorption isaproblem. Vitamin A - Retinol Retinol (vitamin A) Someuses: Vision (11-cis-retinol bound to rhodopsin detectslight in our eyes). Regulating genetranscription (retinoic acid receptorson cell nuclei arepart of a system for regulating transcription of mRNAsfor anumber of genes).
Tear film
1. TEAR FILM
2. The outer most layer of the cornea. It is the exposed part of the eyeball. FUNCTION It provide smooth optical surface It serves to keep the surface of cornea and conjunctiva moist. It serves as a lubricant for the preocular surface and lids It transfer oxygen from the air to the cornea Prevent infection due to the presence of antibacterial substance like lysozymes,and other protein. It wash away debris and irritants Provides pathway to WBC in case of injury.
3. LAYERS OF TEAR FILM It consist of three layers: 1.Lipid layer 2.Aqueous layer 3.Mucoid layer 1.LIPID LAYER
Palestine last event orientationfvgnh .pptxRaedMohamed3
An EFL lesson about the current events in Palestine. It is intended to be for intermediate students who wish to increase their listening skills through a short lesson in power point.
Model Attribute Check Company Auto PropertyCeline George
In Odoo, the multi-company feature allows you to manage multiple companies within a single Odoo database instance. Each company can have its own configurations while still sharing common resources such as products, customers, and suppliers.
Honest Reviews of Tim Han LMA Course Program.pptxtimhan337
Personal development courses are widely available today, with each one promising life-changing outcomes. Tim Han’s Life Mastery Achievers (LMA) Course has drawn a lot of interest. In addition to offering my frank assessment of Success Insider’s LMA Course, this piece examines the course’s effects via a variety of Tim Han LMA course reviews and Success Insider comments.
Francesca Gottschalk - How can education support child empowerment.pptxEduSkills OECD
Francesca Gottschalk from the OECD’s Centre for Educational Research and Innovation presents at the Ask an Expert Webinar: How can education support child empowerment?
Read| The latest issue of The Challenger is here! We are thrilled to announce that our school paper has qualified for the NATIONAL SCHOOLS PRESS CONFERENCE (NSPC) 2024. Thank you for your unwavering support and trust. Dive into the stories that made us stand out!
Acetabularia Information For Class 9 .docxvaibhavrinwa19
Acetabularia acetabulum is a single-celled green alga that in its vegetative state is morphologically differentiated into a basal rhizoid and an axially elongated stalk, which bears whorls of branching hairs. The single diploid nucleus resides in the rhizoid.
2024.06.01 Introducing a competency framework for languag learning materials ...Sandy Millin
http://sandymillin.wordpress.com/iateflwebinar2024
Published classroom materials form the basis of syllabuses, drive teacher professional development, and have a potentially huge influence on learners, teachers and education systems. All teachers also create their own materials, whether a few sentences on a blackboard, a highly-structured fully-realised online course, or anything in between. Despite this, the knowledge and skills needed to create effective language learning materials are rarely part of teacher training, and are mostly learnt by trial and error.
Knowledge and skills frameworks, generally called competency frameworks, for ELT teachers, trainers and managers have existed for a few years now. However, until I created one for my MA dissertation, there wasn’t one drawing together what we need to know and do to be able to effectively produce language learning materials.
This webinar will introduce you to my framework, highlighting the key competencies I identified from my research. It will also show how anybody involved in language teaching (any language, not just English!), teacher training, managing schools or developing language learning materials can benefit from using the framework.
TESDA TM1 REVIEWER FOR NATIONAL ASSESSMENT WRITTEN AND ORAL QUESTIONS WITH A...
Biochemistry of Cerebrospinal fluid (CNF)
1. Biochemistry of CEREBROSPINAL
FLUID
CSF COMPOSITION
Normal CSF; clear, colorless, and odorless
Area Appearance Pressure Cells Protein Miscellaneou
s
Lumbar Clear/colorless 70-180 0-5
(lymphocytes)
<50 mg/dl Glucose
50-75 mg/dl
Ventricular Clear/colorless 70-190 0-5
(lymphocytes)
5-15 mg/dl
Normal CSF values:
2.
3. CEREBROSPINAL FLUID
CLINICAL CONSIDERATIONS
Noncommunicating
(obstructive) hydrocephalus
occurs more frequently
CSF of ventricles unable to
reach subarachnoid space
Production of CSF continues
Gyri are flattened against
inside of skull
If skull is still pliable head may
enlarge
4. CEREBROSPINAL FLUID
CLINICAL CONSIDERATIONS
Communicating
hydrocephalus; obstruction is
in subarchnoid space due to
thickening of the arachnoid
with resultant block of return-
flow channels
Can be the result of prior
bleeding or meningitis
If ICP is increased due to
excess CSF, central canal of
spinal cord may dilate
6. CIRCULATION OF CSF
Circulation: CSF is mainly formed in choroid pleaxus of the
lateral ventricle.
CSF passes from the lateral ventricle to the third ventricle
through the interventricular foramen (foramen of Monro).
From third ventricle it passes to the fourth ventricle through
the cerebrol aqueduct. The circulation is aided by the arterial
pulsations of the choroid plexuses.
From the fourth ventricle (CSF) passes to the subarachnoid
space around the brain and spinal cord through the foramen
of magendie and foramina of luschka.
7. CIRCULATION OF CSF
Lateral ventricle
Foramen of Monro [Interventricular foramen]
Third ventricle:
Subarachnoid space of Brain and Spinal cord
Fourth ventricle:
Cerebral aqueduct
Foramen of megendie and formen of luschka
8. CIRCULATION OF CSF
Circulation: CSF slowly moves cerebromedullary
cistern and pontine cisterns and flows superiorly
through the interval in the tentorium cerebelli to
reach the inferior surface of the cerebrum. It moves
superiority over the lateral aspect of each cerebrol
hemisphere.
9. FUNCTIONS OF CSF
A shock absorber
A mechanical buffer
Act as cushion between the brain and cranium
Act as a reservoir and regulates the contents of the cranium
Serves as a medium for nutritional exchange in CNS
Transport hormones and hormone releasing factors
Removes the metabolic waste products through absorption
10. CSF AND INFLAMMATION
Increased inflammatory cells [pleocytosis] may
be caused by infectious and noninfectious processes.
Polymorphonuclear pleocytosis indicates acute
suppurative meningitis.
Mononuclear cells are seen in viral infections
(meningoencephalitis, aseptic meningitis), syphilis,
neuroborreliosis, tuberculous meningitis, multiple
sclerosis, brain abscess and brain tumors.
11. CSF AND INFLAMMATION
Increased inflammatory cells [pleocytosis] may
be caused by infectious and noninfectious processes.
Polymorphonuclear pleocytosis indicates acute
suppurative meningitis.
Mononuclear cells are seen in viral infections
(meningoencephalitis, aseptic meningitis), syphilis,
neuroborreliosis, tuberculous meningitis, multiple
sclerosis, brain abscess and brain tumors.
12. CSF AND PROTEINS
Increased protein: CSF protein may rise to 500 mg/dl in
bacterial meningitis.
A more moderate increase (150-200 mg/dl) occurs in
inflammatory diseases of meninges (meningitis,
encephalitis), intracranial tumors, subarachnoid
hemorrhage, and cerebral infarction.
A more severe increase occurs in the Guillain-Barré
syndrome and acoustic and spinal schwannoma.
13. CSF AND PROTEINS
Multiple sclerosis: CSF protein is normal or mildly
increased.
Increased IgG in CSF, but not in serum [IgG/albumin index
normally 10:1].
90% of MS patients have oligoclonal IgG bands in the CSF.
Oligoclonal bands occur in the CSF only not in the serum.
The CSF in MS often contains myelin fragments and myelin
basic protein (MBP).
MBP can be detected by radioimmunoassay. MBP is not
specific for MS. It can appear in any condition causing
brain necrosis, including infarcts.
14. CSF & LOW GLUCOSE
Low glucose in CSF:
This condition is seen in suppurative tuberculosis
Fungal infections
Sarcoidosis
Meningeal dissemination of tumors.
Glucose is consumed by leukocytes and tumor cells.
15. BLOOD IN CSF
Blood: Blood may be spilled into the CSF by accidental
puncture of a leptomeningeal vein during entry of the LP
needle.
Such blood stains the fluid that is drawn initially and
clears gradually. If it does not clear, blood indicates
subarachnoid hemorrhage.
Erythrocytes from subarachnoid hemorrhage are cleared
in 3 to 7 days. A few neutrophils and mononuclear cells
may also be present as a result of meningeal irritation.
17. CSF AND XZNTHOCHROMIA
Xanthochromia [blonde color] of the CSF
following subarachnoid hemorrhage is due to
oxyhemoglobin which appears in 4 to 6 hours and
bilirubin which appears in two days.
Xanthochromia may also be seen with hemorrhagic
infarcts, brain tumors, and jaundice.
18. CSF AND TUMOUR CELLS
Tumor cells indicate dissemination of metastatic or
primary brain tumors in the subarachnoid space.
The most common among the latter is medulloblastoma.
They can be best detected by cytological examination.
A mononuclear inflammatory reaction is often seen in
addition to the tumor cells.
19. Indications
In medicine, a lumbar puncture is a diagnostic
in order to collect a sample of cerebrospinal fluid
(CSF) and therapeutic procedure:
Diagnostic for: biochemical, microbiological,
and cytological analysis
Therapeutic for: relieving increased
intracranial pressure, and injecting medication
intarthecally for spinal anesthesia and
chemotherapy.
INDICATIONS OF CSF EXAMINATION
20. CONTRA-INDICATIONS FOR LP
Local skin infections over proposed puncture site
(absolute contra-indication)
Raised intracranial pressure (ICP); exception is
pseudotumor cerebri
Suspected spinal cord mass or intracranial mass
lesion (based on lateralizing neurological findings or
papilledema)
Uncontrolled bleeding diathesis
Spinal column deformities (may require fluoroscopic
assistance)
Lack of patient cooperation
21. LUMBAR PUNCTURE
A lumbar puncture also called a spinal tap is a
procedure where a sample of cerebrospinal fluid is
taken for examination.
CSF is mainly used to diagnose meningitis [an
infection of the meninges].
It is also used to diagnose some other conditions
of the brain and spinal cord.
22. PRECAUTIONS FOR LUMBAR PUNCTURE
Asked to sign a consent form
Ask about taking any medicines
Are allergic to any medicines
Have / had any bleeding problems
Ask about medications such as aspirin or warfarin
Ask the female patient might be pregnant
Empty the bladder before the procedure
23. LUMBAR PUNCTURE
1. Material for sterile technique [gloves and mask
are necessary]
2. Spinal Needle, 20 and 22-gauge
3. Manometer
4. Three-way stopcock
5. Sterile drapes
6. 1% lidocaine without epinephrine in a 5-cc
syringe with a 22 and 25-gauge needles
7. Material for skin sterilization
8. Adhesive dressing
9. Sponges - 10 X 10 cm
24. LUMBAR PUNCTURE [Complications]
Post lumbar puncture headache occurs in 10% to 30% of
patients within 1 to 3 days and lasts 2 to 7 days.
The pain is relieved by lying flat.
Treatment consists of bed rest and fluid with simple
analgesics.
25. LUMBAR PUNCTURE [Complications]
Headache following a lumbar puncture is a common and
often debilitating syndrome.
Continued leakage of cerebrospinal fluid from a puncture
site decreases intracranial pressure, which leads to
traction on pain-sensitive intracranial structures.
The headache is characteristically postural, often
associated with nausea and optic, vestibular, or otic
symptoms. Although usually self-limited after a few days,
severe postural pain can incapacitate the patient.
Management is mainly symptomatic, but definitive
treatment with the epidural blood patching technique is
safe and effective when done by an expert operator.
26. LUMBAR PUNCTURE
Patient usually lie on a bed on side with knees pulled
up against the chest.
It may also done with sitting up and leaning forward on
some pillows. Sterilize the area.
push a needle through the skin and tissues between
two vertebra into the space around the spinal cord
which is filled with CSF.
CSF leaks back through the needle and is collected in a
sterile container.
As soon as the required amount of fluid is collected the
needle is taken out and a plaster is put over the site of
needle entry.
27. LUMBAR PUNCTURE
Sent the sample to lab to be examined under
the microscope to look for bacteria.
It is also 'cultured' for any bacterial growth
The fluid can also be tested for protein, sugar
and other chemicals if necessary.
Sometimes also measure the pressure of the
fluid. This is done by attaching a special tube to
the needle which can measure the pressure of
the fluid coming out.
35. LUMBAR PUNCTURE
Place the patient in the lateral decubitus position lying on
the edge of the bed and facing away from operator.
Place the patient in a knee-chest position with the neck
flexed.
The patient's head should rest on a pillow, so that the
entire cranio-spinal axis is parallel to the bed.
Sitting position is the second choice because there may
be a greater risk of herniation and CSF pressure cannot
be measured
36. LUMBAR PUNCTURE
Find the posterior iliac crest and palpate the L4 spinous
process, and mark the spot with a fingernail.
Prepare the skin by starting at the puncture site.
Anesthetize the skin using the 1% lidocaine in the 5 mL
syringe with the 25-gauge needle. Change to 22-gauge
needle before anesthetizing between the spinous process.
Insert in the midline with the needle parallel to the floor
and the point directed toward the patient's umbilicus
37. LUMBAR PUNCTURE
Advance slowly about 2 cm or until a "pop'' (piercing a
membrane of the dura) is heard.
Then withdraw the stylet in every 2- to 3-mm advance of
the needle to check for CSF return.
If the needle meets the bone or if blood returns (hitting
the venous plexus anterior to the spinal canal), withdraw
to the skin and redirect the needle.
If CSF return cannot be obtained, try one disk space
down