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Base Editing
"From Single Nucleotide
Polymorphisms to Disease
Correction"
Genome Base Editing (BE)
• It’s a technique that allows for precise changes to individual
nucleotides in the DNA sequence without creating DSBs
• It converts one base pair into another without requiring DNA
cleavage
• It’s a new technology for precise modification of DNA or RNA of
living cells at the single base resolution
BE
2 Classes of DNA Base
Editors
CBEs
“Cytosine Base Editors”
ABEs
“Adenine Base Editors”
Cytosine base editing (CBE)
Cytidine
deaminase
nCas9 UGI
Adenine base editing (ABE)
Heterodimeric
deaminase
nCas9
Dual base editor
Adenine
deaminase
nCas9
Cytidine
deaminase
UGI
Prime Editing (PE)
nCas9
Engineered
Reverse
Transcriptase (RT)
CRISPR /Cas13 RNA targeting
systems
NA
Diting for
programmable
to
eplacemet
NA
Diting for
programmable
to
eplacemet
CRISPR /Cas13 RNA targeting
systems
NA
diting for
pecific
to
xchange
NA
diting for
pecific
to
xchange
Organelle editing-based
Organelle editing-based
DddA UGI
TALE
array
DddA cytidine
deaminase (DdCBE), a
UGI to catalyse
cytosine
deamination, inducing
C-to-T conversions and
mitochondrial targeting
signal (MTS) for editing
mitochondrial DNA
Chloroplast transit
peptide (CTP) for
editing Chloroplast
DNA

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Base editing, prime editing, Cas13 & RNA editing and organelle base editing