Dive into the intricate world of molecular genetics with our comprehensive guide to Restriction Fragment Length Polymorphism (RFLP). This PDF unfolds the underlying principles, applications, and significance of RFLP as a molecular marker, offering a thorough exploration of its role in genetic research. Embark on a journey of genetic discovery with our comprehensive guide to RFLP. Whether you are a seasoned researcher or a curious enthusiast, this PDF promises to unravel the mysteries of RFLP and its enduring impact on genetic research.

1. Restriction
Fragment
Length
Polymorphism

2. • Genetic markers resulting from the
variation or change in the length of
defined DNA fragments produced by
digestion of the DNA sample with
restriction endonucleases.
• Use: Southern blots

6. Advantages
• Variants are Co-dominant
• Measures variation at the level
of DNA sequence, not protein
sequence
• Ubiquitous ( large number of
markers , probe –enzyme
combinations .)
Disadvantages
• Labor intensive
• Requires relatively large
amounts of DNA
• Time consuming .

7. DNA markers
Hybridization
based markers
RFLPs Minisatellites Microsatellites
PCR-based
markers
RAPDs MP-PCR AFLPs SSRs SCoT