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RNA organic extraction
guanidinium-acid-phenol method
• Guanidinium thiocyanate-phenol-chloroform extraction method is
often referred to as a conventional method of RNA isolation
• known as the Trizol method of RNA isolation (Guanidinium
thiocyanate and phenol mixture is popularly known as Trizol reagent)
• the sample is treated with :
❑guanidinium isothiocyanate
❑ acidic phenol
❑ chloroform
Advantages Disadvantages
High yield of RNA Compared to
other RNA isolation protocols
• Time consuming
This method is particularly
advantageous in situations
where cells or tissues are
enriched for endogenous
RNases
• Many harmful chemicals compared
to column based methods
Biochemical and molecular techniques
qRT-PCR
cDNA library
construction
northern
blotting
transcriptome
studies
array analysis
References
• https://pubmed.ncbi.nlm.nih.gov/20516177/
RNA organic extraction.pdf

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