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BIOBUTANOL-
AN ADVANCED BIOFUEL
GUIDED BY PRESENTED BY
Dr GIRIDHAR R. NAIR ANJANA S. NAIR
Roll No:7102
CONTENTS
 What is a Biofuel ??
 Bioethanol
 Biodiesel
 Introduction to biobutanol
 Strains and substrate used and corresponding yields
 Genetic engineering in biobutanol production
 Bioreactors for biobutanol production
 Bioseparation
 Biobutanol production and major challenges
 Conclusion
 Recommendations
 Reference
What is a BIOFUEL??
 Type of energy derived from renewable plant and animal
material
 Burns cleanly
 Commonly used biofuels:-
 Ethanol
 Biodiesel
 Biobutanol
BIOETHANOL
 Alcohol
 Commonly made from corn or sugarcane
 Used as an engine fuel
BIOETHANOL
ADVANTAGES
 Can be blended with other fossil fuels
 Reduces greenhouse gases
 Biodegradable
DISADVANTAGES
 Needs large amount of land to produce biomass
 More than 10% ethanol content is not compatible
BIODIESEL
 A substitute for diesel fuel
 Commonly made from soybeans, based on oil
 Used in trucks, diesel cars, etc.
BIODIESEL
ADVANTAGES
 Performs just like normal diesel
 Causes less air pollution and is biodegradable.
 Does not contain sulphur
 Produces 78% less carbon dioxide than diesel
DISADVANTAGES
 More expensive than diesel
 Attracts moisture
BIOFUELS
BIOETHANOL
BIOGAS
BIODIESELBIOBUTANOL
INTRODUCTION TO BIOBUTANOL
 Second generation alcoholic fuel
 Has higher
> energy density
> polarity
> combustion value
> octane rating
 Less corrosive
 Less volatile
INTRODUCTION TO BIOBUTANOL
 Produced by fermentation
 Cost depends on the price of feed stock
 Cheaper sources are agricultural wastes, cheese whey, etc.
 Separation by distillation
INTRODUCTION TO BIOBUTANOL
PRODUCTION DIFFICULTIES
 Product Inhibition
 Anaerobic fermentation
 Formation of azeotrops during separation
PROPERTIES OF BIOBUTANOL
ALTERNATIVE NAMES 1-Butanol,Butyl alcohol, Butyl hydroxide,
Methylopropane, propylcarbinol
IUPAC NAME Butan-1-ol
MOLECULAR FORMULA C4H10O
MOLAR MASS 74.122 g / mol
DENSITY 0.8098 g/cm3
VISCOSITY -89.5oC
BOILING POINT 117.7oC
FLASH POINT 35OC
ADVANTAGES OF BIOBUTANOL
 Can be blended with gasoline in higher amounts
 Can be transported via existing infrastructure
 Can be produced using same fermentation plants and
same sugar and cellulosic feedstock as ethanol
 Has a higher theoretical yield than glucose or other
glucose equivalents
ADVANTAGES OF BIOBUTANOL
 Enhanced tolerance to water contamination
 Better fuel economy due to higher energy density
 Oxygenated fuels can reduce HC- and CO-emissions
because of improved combustion efficiency
DIFFERENT STRAINS AND SUBSTRATE
USED AND CORRESPONDING YIELDS
MICROORGANISM SUBSTRATE YIELD
 C. acetobutylicum Cheese whey 15% for 0.54 h-1
dilution
Cassava bagasse 32% ,76.4 g.l-1
 C. beijerinckii Corn Stover 43%,18.04 g.l-1
of total solvent
Glucose 17.54 g.l-1 butanol
MICROORGANISMS SUBSTRATE YIELD
C. sporogenes
Rice straw 3.49 g.l-1 butanol,
5.32 g. l -1 total
solvent
C. pasteurianum
Glycerol 8.8 g.l-1
DIFFERENT STRAINS AND SUBSTRATE
USED AND CORRESPONDING YIELDS
METABOLISM OF CLOSTRIDIA STRAIN
 Acidogenesis
- substrate acids
 Solventogenesis
-substrates & acid solvents
GENETIC ENGINEERING IN
BIOBUTANOL PRODUCTION
Modified strains
Inserting over expressing genes
Introducing genes of C. acetobutylicum
into E. coli and yeast
BIOREACTORS FOR BIOBUTANOL
PRODUCTION
 Anaerobic bioreactors.
 Operations for production can be accomplished in
> batch
> fed-batch
> continuous modes
 Continuous packed bed reactors can be used.
 Batch reactor is preferred.
Source
Reactor
Clostridium
Biobutanol
BIOREACTORS
BIOSEPERATION
 Mainly by distillation
 Continuous recovery by
 Adsorption
 Gas stripping
 Liquid-liquid interaction
 Flash fermentation
BIOSEPERATION- FLOW CHART
PRODUCTION – FLOW CHART
BIOBUTANOL PRODUCERS
 BUTAMAXTM
 CATHAY
 COBALT TECHNOLOGIES
MAJOR CHALLENGES FOR
BIOBUTANOL
 Low butanol concentration
 Product inhibition
 Expensive
CONCLUSION
 An advanced biofuel
 Has many advantages
 Still there are problems to rectify
 Commercially produced in Europe and North America
 Asia Pacific and rest of the world is still depending on
conventional fossil fuels
 Energy crisis afflicted country like India can produce biobutanol
commercially
 Can be produced from banana peel, bamboo, waste paper,
vegetable skins, etc.
 Aerobic fermentation using TU- 103 , a clostridium sp.
RECOMMENDATIONS
REFERENCES
1. Luiz J Visioli, Heveline Enzweiler, Raquel C Kuhn, Marcio Schwaab and Marcio
A Mazutti: Recent advances on biobutanol production, 2014; 2:15, 97105-
900.
2. Durre P. Biobutanol: an attractive biofuel. Biotechnol J. 2007;2:1525-34.
3. Kumar M, Gayen K: Developments in biobutanol production: New insights.
Appl Energ 2011, 88:1999–2012.
4. Chen WH, Chen YC, Lin JG: Evaluation of biobutanol production from non-
pretreated rice straw hydrolysate under non-sterile environmental conditions.
Bioresour Technol 2013, 135:262–268.
THANK YOU

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BIOBUTANOL- AN ADVANCED BIOFUEL

  • 1. BIOBUTANOL- AN ADVANCED BIOFUEL GUIDED BY PRESENTED BY Dr GIRIDHAR R. NAIR ANJANA S. NAIR Roll No:7102
  • 2. CONTENTS  What is a Biofuel ??  Bioethanol  Biodiesel  Introduction to biobutanol  Strains and substrate used and corresponding yields  Genetic engineering in biobutanol production  Bioreactors for biobutanol production  Bioseparation  Biobutanol production and major challenges  Conclusion  Recommendations  Reference
  • 3. What is a BIOFUEL??  Type of energy derived from renewable plant and animal material  Burns cleanly  Commonly used biofuels:-  Ethanol  Biodiesel  Biobutanol
  • 4. BIOETHANOL  Alcohol  Commonly made from corn or sugarcane  Used as an engine fuel
  • 5. BIOETHANOL ADVANTAGES  Can be blended with other fossil fuels  Reduces greenhouse gases  Biodegradable DISADVANTAGES  Needs large amount of land to produce biomass  More than 10% ethanol content is not compatible
  • 6. BIODIESEL  A substitute for diesel fuel  Commonly made from soybeans, based on oil  Used in trucks, diesel cars, etc.
  • 7. BIODIESEL ADVANTAGES  Performs just like normal diesel  Causes less air pollution and is biodegradable.  Does not contain sulphur  Produces 78% less carbon dioxide than diesel DISADVANTAGES  More expensive than diesel  Attracts moisture
  • 9. INTRODUCTION TO BIOBUTANOL  Second generation alcoholic fuel  Has higher > energy density > polarity > combustion value > octane rating  Less corrosive  Less volatile
  • 10. INTRODUCTION TO BIOBUTANOL  Produced by fermentation  Cost depends on the price of feed stock  Cheaper sources are agricultural wastes, cheese whey, etc.  Separation by distillation
  • 11. INTRODUCTION TO BIOBUTANOL PRODUCTION DIFFICULTIES  Product Inhibition  Anaerobic fermentation  Formation of azeotrops during separation
  • 12. PROPERTIES OF BIOBUTANOL ALTERNATIVE NAMES 1-Butanol,Butyl alcohol, Butyl hydroxide, Methylopropane, propylcarbinol IUPAC NAME Butan-1-ol MOLECULAR FORMULA C4H10O MOLAR MASS 74.122 g / mol DENSITY 0.8098 g/cm3 VISCOSITY -89.5oC BOILING POINT 117.7oC FLASH POINT 35OC
  • 13. ADVANTAGES OF BIOBUTANOL  Can be blended with gasoline in higher amounts  Can be transported via existing infrastructure  Can be produced using same fermentation plants and same sugar and cellulosic feedstock as ethanol  Has a higher theoretical yield than glucose or other glucose equivalents
  • 14. ADVANTAGES OF BIOBUTANOL  Enhanced tolerance to water contamination  Better fuel economy due to higher energy density  Oxygenated fuels can reduce HC- and CO-emissions because of improved combustion efficiency
  • 15. DIFFERENT STRAINS AND SUBSTRATE USED AND CORRESPONDING YIELDS MICROORGANISM SUBSTRATE YIELD  C. acetobutylicum Cheese whey 15% for 0.54 h-1 dilution Cassava bagasse 32% ,76.4 g.l-1  C. beijerinckii Corn Stover 43%,18.04 g.l-1 of total solvent Glucose 17.54 g.l-1 butanol
  • 16. MICROORGANISMS SUBSTRATE YIELD C. sporogenes Rice straw 3.49 g.l-1 butanol, 5.32 g. l -1 total solvent C. pasteurianum Glycerol 8.8 g.l-1 DIFFERENT STRAINS AND SUBSTRATE USED AND CORRESPONDING YIELDS
  • 17. METABOLISM OF CLOSTRIDIA STRAIN  Acidogenesis - substrate acids  Solventogenesis -substrates & acid solvents
  • 18. GENETIC ENGINEERING IN BIOBUTANOL PRODUCTION Modified strains Inserting over expressing genes Introducing genes of C. acetobutylicum into E. coli and yeast
  • 19. BIOREACTORS FOR BIOBUTANOL PRODUCTION  Anaerobic bioreactors.  Operations for production can be accomplished in > batch > fed-batch > continuous modes  Continuous packed bed reactors can be used.  Batch reactor is preferred.
  • 21. BIOSEPERATION  Mainly by distillation  Continuous recovery by  Adsorption  Gas stripping  Liquid-liquid interaction  Flash fermentation
  • 24. BIOBUTANOL PRODUCERS  BUTAMAXTM  CATHAY  COBALT TECHNOLOGIES
  • 25.
  • 26.
  • 27. MAJOR CHALLENGES FOR BIOBUTANOL  Low butanol concentration  Product inhibition  Expensive
  • 28. CONCLUSION  An advanced biofuel  Has many advantages  Still there are problems to rectify  Commercially produced in Europe and North America  Asia Pacific and rest of the world is still depending on conventional fossil fuels
  • 29.  Energy crisis afflicted country like India can produce biobutanol commercially  Can be produced from banana peel, bamboo, waste paper, vegetable skins, etc.  Aerobic fermentation using TU- 103 , a clostridium sp. RECOMMENDATIONS
  • 30. REFERENCES 1. Luiz J Visioli, Heveline Enzweiler, Raquel C Kuhn, Marcio Schwaab and Marcio A Mazutti: Recent advances on biobutanol production, 2014; 2:15, 97105- 900. 2. Durre P. Biobutanol: an attractive biofuel. Biotechnol J. 2007;2:1525-34. 3. Kumar M, Gayen K: Developments in biobutanol production: New insights. Appl Energ 2011, 88:1999–2012. 4. Chen WH, Chen YC, Lin JG: Evaluation of biobutanol production from non- pretreated rice straw hydrolysate under non-sterile environmental conditions. Bioresour Technol 2013, 135:262–268.