3. 1-D NMR
1. Simple FT-NMR Technique
2. 1-D NMR spectroscopy is simple technique In which we provide 90 degree pulse to sample
which is placed in uniform magnetic field. As a result we get a FID signal (raw form). To get
meaningful data we apply Fourier Transform program on FID to get desire signal.
3. Data in form of frequency vs intensity is plotted along x-axis and y-axis respectively
4. Sequence Scheme
If we divide the 1-D NMR into a general periods , we get two steps
•Preparation: (do something with nuclei)
1.What we can do ???
•Simply we provide a 90 degree pulse
2.Detection: ( detect the signal)
.Simply we measure the signal
5. Why Named 1-D NMR ??
• We call it 1-D NMR , not because of graph. Graph is actually in 2-d.
• Since one of the time domain is changing into frequency dimension
Thats why we call it 1D NMR .
6.
7. • The first two-dimensional experiment was proposed by
Jean Jenner
. • 2D NMR is a set of nuclear magnetic resonance
spectroscopy (NMR) methods which give data plotted in a
space defined by two frequency axes rather than one.
• 2D NMR spectra provide more information about a
molecule than one-dimensional NMR spectra
2-D NMR
8. Principle :
2D NMR is Nuclear magnetic resonance spectroscopy in which we
provide a 90 degree pulse to our sample which is placed in uniform
magnetic field then we wait for (0ms-5ms) depending upon the experiment
type then again we provide again 90 degree pulse and this time we measure
signal as FID.
As a result we get Two FID signals which are plotted with respect to time
domain.
To get a meaningful data , we apply Fourier transform program on FIDs to
get frequency in terms of ppm.
9. • Primary 2D matrix consists a series of FIDs
• A set of 1D NMR spectra is obtained by Fourier transformation with respect
to t2.
• The signals of each transformation may differ in amplitude and phase. A
second Fourier transformation with respect to t1 yields the final 2D matrix
with frequency axes F1 and F2
