X-ray crystallography is a scientific technique used to determine the atomic and molecular structure of crystals. When x-rays strike a crystal, the beam diffracts into specific directions. This diffraction pattern can be analyzed to reveal the nature and structure of the crystal lattice. Bragg's law defines the relationship between x-ray wavelength, diffraction angle, and interplanar spacing and is used to calculate crystal structures from diffraction data. X-ray crystallography is widely used to determine protein structures and has applications in pharmaceuticals, materials science, and other fields.
X-ray Crystallography is a scientific method used to determine the arrangement of atoms of a crystalline solid in three dimension. It is based on x ray diffraction. Reveals structure of a crystal at atomic level.
X ray crystallography and X ray DiffractionFaisal Hussain
This is the short description about x ray crystallography.
simplest and easy to understand.
Procedure of X ray Diffraction.
Pros and Cons of X ray Crystallography
X-Ray Crystallography is a technique used to determine the atomic and molecular structure of a crystal, in which the crystalline atoms cause a beam of incident X-rays to diffract into many specific directions.
X ray crystallography and X ray DiffractionFaisal Hussain
This is the short description about x ray crystallography.
simplest and easy to understand.
Procedure of X ray Diffraction.
Advantages and Disadvantages of X ray Crystallography
X-ray Crystallography is a scientific method used to determine the arrangement of atoms of a crystalline solid in three dimension. It is based on x ray diffraction. Reveals structure of a crystal at atomic level.
X ray crystallography and X ray DiffractionFaisal Hussain
This is the short description about x ray crystallography.
simplest and easy to understand.
Procedure of X ray Diffraction.
Pros and Cons of X ray Crystallography
X-Ray Crystallography is a technique used to determine the atomic and molecular structure of a crystal, in which the crystalline atoms cause a beam of incident X-rays to diffract into many specific directions.
X ray crystallography and X ray DiffractionFaisal Hussain
This is the short description about x ray crystallography.
simplest and easy to understand.
Procedure of X ray Diffraction.
Advantages and Disadvantages of X ray Crystallography
X ray, invisible, highly penetrating electromagnetic radiation of much shorter wavelength (higher frequency) than visible light. The wavelength range for X rays is from about 10-8 m to about 10-11 m, the corresponding frequency range is from about 3 × 1016 Hz to about 3 × 1019 Hz.
X ray, invisible, highly penetrating electromagnetic radiation of much shorter wavelength (higher frequency) than visible light. The wavelength range for X rays is from about 10-8 m to about 10-11 m, the corresponding frequency range is from about 3 × 1016 Hz to about 3 × 1019 Hz.
Production of X rays, Different X ray diffraction methods, Bragg‘s law, Rotating crystal technique, X ray powder technique, Types of crystals and applications of X-ray Diffraction
X ray crystallography to visualize protein structure.Ritam38
This ppt discusses in detail the process of X ray Crystallography.
Made by the following 3rd year Bs-Ms students of IISER Kolkata:
B Sri Sindhu
Rasiwala Hassan Shabbir
Ritam Samanta
Himanshu Gupta
Sakshi Ajay Shrisath
Aditya Borkar
Diana Denzil Fernandez
Neha Kumari
.Sowmya
Anjali Mohan
Debanjana Mondal
Aanandita Gope
Shruti Santosh Sail
XRD is a rapid analytical technique primarily used for phase identification of crystalline material and can provide information on unit cell dimensions.
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
Cancer cell metabolism: special Reference to Lactate PathwayAADYARAJPANDEY1
Normal Cell Metabolism:
Cellular respiration describes the series of steps that cells use to break down sugar and other chemicals to get the energy we need to function.
Energy is stored in the bonds of glucose and when glucose is broken down, much of that energy is released.
Cell utilize energy in the form of ATP.
The first step of respiration is called glycolysis. In a series of steps, glycolysis breaks glucose into two smaller molecules - a chemical called pyruvate. A small amount of ATP is formed during this process.
Most healthy cells continue the breakdown in a second process, called the Kreb's cycle. The Kreb's cycle allows cells to “burn” the pyruvates made in glycolysis to get more ATP.
The last step in the breakdown of glucose is called oxidative phosphorylation (Ox-Phos).
It takes place in specialized cell structures called mitochondria. This process produces a large amount of ATP. Importantly, cells need oxygen to complete oxidative phosphorylation.
If a cell completes only glycolysis, only 2 molecules of ATP are made per glucose. However, if the cell completes the entire respiration process (glycolysis - Kreb's - oxidative phosphorylation), about 36 molecules of ATP are created, giving it much more energy to use.
IN CANCER CELL:
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
introduction to WARBERG PHENOMENA:
WARBURG EFFECT Usually, cancer cells are highly glycolytic (glucose addiction) and take up more glucose than do normal cells from outside.
Otto Heinrich Warburg (; 8 October 1883 – 1 August 1970) In 1931 was awarded the Nobel Prize in Physiology for his "discovery of the nature and mode of action of the respiratory enzyme.
WARNBURG EFFECT : cancer cells under aerobic (well-oxygenated) conditions to metabolize glucose to lactate (aerobic glycolysis) is known as the Warburg effect. Warburg made the observation that tumor slices consume glucose and secrete lactate at a higher rate than normal tissues.
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
Richard's entangled aventures in wonderlandRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
PRESENTATION ABOUT PRINCIPLE OF COSMATIC EVALUATION
X ray crystallography
1. STRUCTURAL ANALYSIS BY X-RAY
CRYSTALLOGRAPHY
By
SIJINU MATHEW
Physical Science
Roll no 13
1
2. ELECTRO MAGNETIC SPECTRUM
Electro magnetic spectrum is the entire range of electro
magnetic waves from lower to higher frequency. It
contain various types of radiations.
gamma rays, x-rays, UV-Visible, IR, Micro waves and
radio waves.
2
4. X-RAYS
X-rays have the short wavelengths to see the atoms and
molecular structure of molecules.
Wavelength range is 1-10 Å
Discovered by Wilhelm Rontgen in 1895.
It can be generated by reducing the speed of electrons.
4
5. X-ray Crystallography
It is a scientific method of determining the precise
arrangements of atoms in a crystal where beams of x-ray
strikes a crystal and causes the beam of light to diffract
into specific directions.
5
6. Contd........
This method is widely used to obtain high resolution
protein structural information.
This technique relies the dual nature of x-rays to discover
information about structure of crystalline materials.
The pattern produced by the diffraction of x-rays through
the closely spaced lattice of atoms in a crystal is
recorded and analysed to reveal the nature of that lattice.
6
7. Contd....
The sample that is in crystalline form is bombarded
with beam of x-rays , then most of the x-ray pass
straight through the crystal some are diffracted by it.
The resulting pattern recorded by a detector .
Pattern is the three dimensional structure of sample
This method can give detailed atomic structure such as
about ligands, inhibitors, ions, and other molecules.
7
8. Bragg’s Law
It is a fundamental law and valid for monochromatic x-
rays only and it is used to calculate interplanar spacing
using x-ray diffraction spectra
It defines the spacing(d) of atomic planes and incident
angle(Ө) at which x-rays of a particular wavelength will
reflect in phase.
8
9. 9
nλ = 2d sinθ
The Bragg’s equation is,
nλ=2d sinθ
10. Single X-ray Crystallography
It is the oldest and more precise model
A beam of x-rays strikes a single crystal, producing
scattered beams.
It has three steps,
10
12. Nature of the Crystal
The crystal should be large , pure in composition and
regular in structure, with no significant internal
imperfections such as crack or twinning.
The crystal placed in an intense beam of x-rays , usually
single wavelength producing regular pattern of
reflections.
12
13. Contd......
When the crystal is rotated that time reflection will
change and intensity will be recorded.
These data are combined computationally with
complementary chemical information to produce and
refine a model of the arrangement of atoms with in the
crystal.
13
14. Applications
Determination of unit cell, bond-lengths, bond-angles
Variations in crystal lattice
With specialized chambers, structures of high pressure
and/or temperature phases can be determined
Powder patterns can also be derived from single-crystals
by use of specialized cameras (Gandalf) applications.
14
15. Limitations
The unit cell is larger and more complex.
The atomic level picture provided by x-ray
crystallography become less well resolved.
15
18. 18
Contd……
X-ray generated in the tube T are passed through a slit,
so as to obtained a narrow beam which is then allowed to
strike single crystal which is mounted on the turn table.
The crystal is rotated gradually by means of the turn table
as to increase the glancing angle.
19. Contd…….
The intensities of the reflected rays are measured on a
recording device. The process is carried out for each
plane of the crystal till the angles for reflection θ give the
maximum value.
The lowest angle at which the maximum reflection occurs
corresponds to n= 1. this is first order reflection and so
on.
19
21. Contd….
Used for the crystal with simple structure
Powder contain small crystal that are oriented in all
directions .
As a result x-rays are scattered from all sets of planes.
Scattered rays are detected by using an x-ray sensitive
film.
The finely powdered substance is kept in the form of a
cylinder inside a thin glass tube.
Narrow beam of x-rays is allowed to fall on the powder.21
22. The diffracted x-rays strike a strip of photographic film
arranged in the form of a cylindrical arc.
In this method rotation is not needed because powder
sample contains micro crystals arranged in all possible
orientations.
A large number of them will have their lattice planes in
corrected positions for maximum x-ray reflection to
occur.
We get lighted areas in the form of arcs of lines at
different distances from the incident beam.
22
23. Advantages
Non-destructive nature, high sensitivity,
Easy sample preparation,
System is user friendly, operational procedure is
convenient, fast , effective resolution.
Low maintenance cost, proper automation, easy data
interpretation that could be used for both qualitative and
quantitative analysis.
Disadvantage
Use of harmful radiations.
23
25. Factors Affecting X-ray Crystallography
Purity of sample
Concentration
pH of solution
Temperature
Time
Volume of crystallisation
Pressure
25
26. Advantages and Disadvantages
Advantages
X-rays are the least expensive, most convenient and
widely used to determine crystal structures.
X-rays are not absorbed very much by air, so the sample
need not be in an evacuated chamber.
Disadvantages
X-rays do not interact very strongly with higher elements.
26
27. Applications
Distinguishing between crystalline & amorphous
materials.
Determination of the structure of crystalline materials.
Determination of electron distribution within the atoms &
throughout the unit cell.
27
28. Determination of the orientation of single crystals.
Determination of the texture of polygrained materials.
Measurement of strain and small grain size………
28
29. Medical Applications
In diagnosis & treatment
Tuberculosis& emphysema
Dentists
CT or CAT(computerized axial tomography)
X-ray therapy
29
30. CONCLUSION
The advent of x-ray diffraction in the early 20th century
transformed crystallography from an area of scientific
inquiry largely limited to physics, mineralogy, and
mathematics to a highly interdisciplinary field which now
includes nearly all life and physical sciences as well as
material science and engineering.
30