This document discusses vitamins, specifically thiamine, riboflavin, and niacin. It describes their chemical structures, functions as coenzymes, dietary sources, requirements, and deficiency diseases. Thiamine deficiency causes beriberi, while riboflavin deficiency causes angular stomatitis and glossitis. Niacin deficiency results in pellagra characterized by stomatitis, glossitis, diarrhea, and dermatitis. All three are water-soluble vitamins that must be obtained daily through diet as they are not stored in the body.
This document summarizes information about vitamin B1 (thiamine) and its active coenzyme form, thiamine pyrophosphate (TPP). It discusses the history of thiamine discovery and its chemical structure. Thiamine is converted to TPP in the liver and intestinal mucosa by the enzyme thiamine pyrophosphokinase using ATP. TPP acts as a coenzyme, transferring aldehyde groups in metabolic reactions. Sources of thiamine are mentioned and a deficiency can cause beriberi. Functions include roles in growth, nervous system maintenance, and metabolism.
The anthocyanin is one of the nature's best medicine used to cure cancer and many other diseases. In this slide I've discussed about its anticancer and antioxidant effects, common sources of anthocyanin, its chemo preventive effects etc.It also contains some abstracts from PubMed.
Vitamin B2, also known as riboflavin, is an essential component of coenzymes involved in redox reactions in the body. It acts as a precursor to FMN and FAD, which are cofactors for redox enzymes. Deficiency can result from poor intake, malabsorption, drug interactions, and genetic defects affecting flavin metabolism. Symptoms include sore throat, cheilosis, and glossitis. Status is assessed by urinary riboflavin levels, erythrocyte glutathione reductase activity, and direct measurement of flavins in plasma and erythrocytes.
Pinene is a bicyclic monoterpene compound that occurs as two structural isomers, alpha-pinene and beta-pinene, which are major components of pine tree essential oils. It is the main terpene found in pine trees, which gives them their characteristic scent, and is also present in rosemary and cannabis. Both forms of pinene are produced from geranyl pyrophosphate and have several uses, including as an analgesic, antibacterial, anti-inflammatory, and antioxidant.
Thiamine pyrophosphate (TPP) is an important coenzyme that maintains normal heart and energy metabolism functions. TPP works as a coenzyme in several enzymatic reactions including pyruvate decarboxylase, transketolase, pyruvate dehydrogenase, and alpha ketoglutarate dehydrogenase. As a cofactor for pyruvate decarboxylase, TPP facilitates the decarboxylation of pyruvate to acetaldehyde. For transketolase, TPP transfers a two-carbon unit from xylulose 5-phosphate to ribose 5-phosphate, yielding sedoheptulose 7-phosphate and glyceraldehyde 3-phosphate. The mechanisms of these reactions
Aminiacid Selenocysteine and PyrrolysineShwetA Kumari
This presentation summarizes the 21st and 22nd amino acids, selenocysteine and pyrrolysine. Selenocysteine contains selenium in place of sulfur and is encoded by the UGA codon. It plays an important role in antioxidant enzymes. Pyrrolysine is a recently discovered 22nd amino acid encoded by UAG codon. It contains a pyrroline ring and is used by some archaea in methane production. Both expand the genetic code beyond the standard 20 amino acids.
Thiamine is also called as vitamin B1, Aneurine (it can relieve neuritis), or antiberberi
factor.
• In 1900, Christian Eijkman produced beriberi in chicken by feeding polished rice (Nobel
Prize, 1929).
• Adolf Windaus (Nobel Prize, 1928) elucidated the structure of the vitamin.
Structure of Thiamine:
Biosynthesis:
Deficiency Manifestations of Thiamine
Thiamine, also known as thiamin or vitamin B₁, is a vitamin found in food and manufactured as a dietary supplement and medication. Food sources of thiamine include whole grains, legumes, and some meats and fish
The document discusses aromatic amino acids (AAA), which include phenylalanine, tryptophan, histidine, and tyrosine. All plants and microorganisms must synthesize AAA through the shikimate pathway, unlike animals which obtain them through diet. The shikimate pathway is a seven step route that produces chorismate, the precursor to the AAA. The document then discusses the properties, biosynthesis through the shikimate pathway, and catabolism of each individual AAA - phenylalanine, tryptophan, histidine, and tyrosine.
This document summarizes information about vitamin B1 (thiamine) and its active coenzyme form, thiamine pyrophosphate (TPP). It discusses the history of thiamine discovery and its chemical structure. Thiamine is converted to TPP in the liver and intestinal mucosa by the enzyme thiamine pyrophosphokinase using ATP. TPP acts as a coenzyme, transferring aldehyde groups in metabolic reactions. Sources of thiamine are mentioned and a deficiency can cause beriberi. Functions include roles in growth, nervous system maintenance, and metabolism.
The anthocyanin is one of the nature's best medicine used to cure cancer and many other diseases. In this slide I've discussed about its anticancer and antioxidant effects, common sources of anthocyanin, its chemo preventive effects etc.It also contains some abstracts from PubMed.
Vitamin B2, also known as riboflavin, is an essential component of coenzymes involved in redox reactions in the body. It acts as a precursor to FMN and FAD, which are cofactors for redox enzymes. Deficiency can result from poor intake, malabsorption, drug interactions, and genetic defects affecting flavin metabolism. Symptoms include sore throat, cheilosis, and glossitis. Status is assessed by urinary riboflavin levels, erythrocyte glutathione reductase activity, and direct measurement of flavins in plasma and erythrocytes.
Pinene is a bicyclic monoterpene compound that occurs as two structural isomers, alpha-pinene and beta-pinene, which are major components of pine tree essential oils. It is the main terpene found in pine trees, which gives them their characteristic scent, and is also present in rosemary and cannabis. Both forms of pinene are produced from geranyl pyrophosphate and have several uses, including as an analgesic, antibacterial, anti-inflammatory, and antioxidant.
Thiamine pyrophosphate (TPP) is an important coenzyme that maintains normal heart and energy metabolism functions. TPP works as a coenzyme in several enzymatic reactions including pyruvate decarboxylase, transketolase, pyruvate dehydrogenase, and alpha ketoglutarate dehydrogenase. As a cofactor for pyruvate decarboxylase, TPP facilitates the decarboxylation of pyruvate to acetaldehyde. For transketolase, TPP transfers a two-carbon unit from xylulose 5-phosphate to ribose 5-phosphate, yielding sedoheptulose 7-phosphate and glyceraldehyde 3-phosphate. The mechanisms of these reactions
Aminiacid Selenocysteine and PyrrolysineShwetA Kumari
This presentation summarizes the 21st and 22nd amino acids, selenocysteine and pyrrolysine. Selenocysteine contains selenium in place of sulfur and is encoded by the UGA codon. It plays an important role in antioxidant enzymes. Pyrrolysine is a recently discovered 22nd amino acid encoded by UAG codon. It contains a pyrroline ring and is used by some archaea in methane production. Both expand the genetic code beyond the standard 20 amino acids.
Thiamine is also called as vitamin B1, Aneurine (it can relieve neuritis), or antiberberi
factor.
• In 1900, Christian Eijkman produced beriberi in chicken by feeding polished rice (Nobel
Prize, 1929).
• Adolf Windaus (Nobel Prize, 1928) elucidated the structure of the vitamin.
Structure of Thiamine:
Biosynthesis:
Deficiency Manifestations of Thiamine
Thiamine, also known as thiamin or vitamin B₁, is a vitamin found in food and manufactured as a dietary supplement and medication. Food sources of thiamine include whole grains, legumes, and some meats and fish
The document discusses aromatic amino acids (AAA), which include phenylalanine, tryptophan, histidine, and tyrosine. All plants and microorganisms must synthesize AAA through the shikimate pathway, unlike animals which obtain them through diet. The shikimate pathway is a seven step route that produces chorismate, the precursor to the AAA. The document then discusses the properties, biosynthesis through the shikimate pathway, and catabolism of each individual AAA - phenylalanine, tryptophan, histidine, and tyrosine.
Metalloenzymes contain metal ions that help catalyze important biochemical reactions. Antioxidants protect cells from oxidative damage caused by free radicals generated during normal metabolism and environmental exposures. There are many classes of antioxidants including vitamins, minerals, enzymes, carotenoids, flavonoids, and phenolic compounds. Antioxidants act as reducing agents that prevent oxidative chain reactions and thereby protect cellular components from oxidative damage.
Thiamine, also known as vitamin B1, is a water-soluble vitamin that plays an important role in energy production and nerve function. It is involved in carbohydrate, fat, and amino acid metabolism as the coenzyme thiamine pyrophosphate. Thiamine deficiency can lead to diseases like beriberi, which has wet, dry, and infantile forms causing symptoms like edema, nerve damage, and heart problems. Other deficiency symptoms include weakness, confusion, and Wernicke-Korsakoff syndrome. Good dietary sources of thiamine include whole grains, legumes, pork, and nuts.
Riboflavin, also known as vitamin B2, is a yellow pigment that contains a 6,7-dimethylisoalloxazine ring. It functions as an important cofactor in redox reactions as FMN and FAD. Riboflavin is absorbed in the small intestine and transported to tissues like the liver, where it is converted to its coenzyme forms. Deficiency can cause cheilosis, glossitis and corneal vascularization. Rich dietary sources include milk, meat, eggs and liver.
Mechanism of action of Chymotrypsin & Lysozyme.pptxVanshikaVarshney5
Chymotrypsin and Lysozyme are the most important enzymes. Mechanism of action of these enzymes and introduction of these enzyme are given in this presentation in simple, easy and understanding language. Hope you will find it useful :)
Introduction to nucleic acid, chemistry of nucleotiides , july 2020enamifat
This document provides an introduction to nucleic acids, their components, and chemistry. It can be summarized as follows:
1. Nucleic acids are high molecular weight polymers composed of nucleotides linked by phosphodiester bonds. The nucleotides contain a pentose sugar, phosphate group, and nitrogenous base.
2. DNA contains deoxyribose and RNA contains ribose. The nitrogenous bases in DNA are adenine, guanine, cytosine, and thymine, while in RNA thymine is replaced by uracil.
3. Nucleotides are the monomers that make up nucleic acids. They contain a nucleoside (pentose sugar + nitrogenous base), and one or more phosphate groups
Absorption, transport and metabolism of pyridoxineDomina Petric
The document summarizes the absorption, transport, and metabolism of pyridoxine (vitamin B6). It states that pyridoxine is absorbed via passive diffusion in the small intestine and transported to the liver bound to albumin in plasma. In tissues and liver, it is phosphorylated to its active form, pyridoxal phosphate. The liver plays a central role in interconverting pyridoxine forms and metabolizing excess amounts, which are ultimately excreted in urine as 4-pyridoxic acid. Alcohol and the drug INH can interfere with pyridoxine metabolism by displacing or binding to pyridoxal phosphate.
Absorption, transport and metabolism of niacinDomina Petric
The document summarizes the absorption, transport, and metabolism of niacin. It discusses how niacin is absorbed in the small intestine through facilitated diffusion. It is transported through the bloodstream as nicotinic acid and nicotinamide and taken up by tissues. Within tissues, it is converted to its coenzyme forms NAD and NADP which are used to drive cellular processes. The document also outlines how humans can synthesize niacin from the amino acid tryptophan through multiple enzymatic steps in the liver.
1) The document discusses coenzymes and vitamins, which are organic molecules that serve as cofactors for enzyme reactions in the body. It covers various coenzymes like ATP, NAD+, FAD, and FMN that are derived from vitamins and transport chemical groups between enzymes.
2) Vitamins are classified as either water-soluble or fat-soluble. Water-soluble vitamins include the B vitamins and vitamin C, while fat-soluble vitamins are vitamins A, D, E, and K. Vitamins function as coenzymes and are essential for growth, development and various metabolic processes.
3) The document provides details on the structures and functions of important coen
Ionophores are molecules that transport ions across biological membranes. They contain both hydrophilic regions that bind ions and hydrophobic regions that interact with membrane lipids. Ionophores are classified based on their mechanism of action as either mobile carrier ionophores which transport ion complexes, or channel-forming ionophores which introduce pores for ion passage. Examples include valinomycin which transports potassium ions, gramicidin A which forms channels for cation transport, and ionomycin which carries calcium ions into cells. Ionophores have important applications as antibiotics, in research to manipulate cellular physiology, and as feed additives to improve livestock growth and productivity.
Significance of shine dalgarno sequencePrajaktaPanda
The shine dalgarno sequence is a ribosomal site in the prokaryotic bacterial mRNA which helps in protein synthesis by aligning the ribosome with the start codon. It's significance deals with it's effect and importance during the translation process within an mRNA.
This document summarizes the isolation of pigments from hibiscus flowers. It describes pigments as water- and oil-insoluble natural or synthetic compounds that impart color. The main pigment in hibiscus is anthocyanin, specifically cyanidin 3-sophoroside. The extraction method involves soaking hibiscus petals in methanol or ethanol with either hydrochloric acid or citric acid. Citric acid extraction yields a larger amount and is more recommended. Nuclear magnetic resonance spectroscopy was used to analyze the extracted pigment. Anthocyanins have various health benefits and are used as food colors, dyes, and pH indicators.
Riboflavin,flavoproteins and their clinical applicationsrohini sane
A presentation in lucid-style on Riboflavin (vitamin B2), Flavoproteins and their clinical applications for MBBS , BDS , B Pham and Biotechnology students to facilitate easy leaning.
Pyridoxine, also known as vitamin B6, is a water-soluble vitamin that exists in three forms - pyridoxine, pyridoxal, and pyridoxamine. All three forms are precursors to pyridoxal phosphate (PLP), the active coenzyme form of vitamin B6. PLP acts as a cofactor in many enzymatic reactions involved in amino acid, lipid, and carbohydrate metabolism. Deficiency of vitamin B6 can result in neurological, dermatological, and hematological symptoms due to its role in many biochemical pathways.
This document provides an overview of protein structure and function. It discusses the four levels of protein structure from primary to quaternary structure. The 20 standard amino acids that serve as building blocks of proteins are described in terms of their chemical and physical properties. Amino acids contain both polar and non-polar side chains that influence protein structure and function. Key concepts covered include protein folding, the zwitterionic nature of amino acids, and interactions like disulfide bonds and hydrophobic effects that stabilize protein tertiary structure.
Post translational modification of protienkamilKhan63
The document discusses post-translational modifications (PTM) of proteins. It defines PTM as the chemical modification of proteins after translation, including phosphorylation, acetylation, methylation, glycocylation, and other types of modifications. These modifications are important as they increase protein diversity and regulate functions like activity, localization, and interactions. The document also describes techniques for detecting PTM, including mass spectrometry and blotting.
Vitamins are organic compounds that are required by humans in small amounts. They are classified as either water-soluble or fat-soluble. Water-soluble vitamins include the B vitamins and vitamin C, while fat-soluble vitamins are vitamins A, D, E, and K. Vitamins function as coenzymes and play important roles in metabolism, energy production, blood cell formation, nerve transmission, gene expression, and other processes. Deficiencies can result in conditions like beriberi, pellagra, scurvy, and rickets.
Vitamins are organic compounds that are required in small amounts to maintain normal health. The word "vitamin" comes from the Latin word "vita" meaning life. There are two types of vitamins - fat soluble (A, D, E, K) and water soluble (B complex, C). Vitamin A is important for vision, epithelial cell integrity, reproduction, resistance to infection, and bone remodeling. Symptoms of vitamin A deficiency include night blindness, dry eyes, and corneal damage.
Metalloenzymes contain metal ions that help catalyze important biochemical reactions. Antioxidants protect cells from oxidative damage caused by free radicals generated during normal metabolism and environmental exposures. There are many classes of antioxidants including vitamins, minerals, enzymes, carotenoids, flavonoids, and phenolic compounds. Antioxidants act as reducing agents that prevent oxidative chain reactions and thereby protect cellular components from oxidative damage.
Thiamine, also known as vitamin B1, is a water-soluble vitamin that plays an important role in energy production and nerve function. It is involved in carbohydrate, fat, and amino acid metabolism as the coenzyme thiamine pyrophosphate. Thiamine deficiency can lead to diseases like beriberi, which has wet, dry, and infantile forms causing symptoms like edema, nerve damage, and heart problems. Other deficiency symptoms include weakness, confusion, and Wernicke-Korsakoff syndrome. Good dietary sources of thiamine include whole grains, legumes, pork, and nuts.
Riboflavin, also known as vitamin B2, is a yellow pigment that contains a 6,7-dimethylisoalloxazine ring. It functions as an important cofactor in redox reactions as FMN and FAD. Riboflavin is absorbed in the small intestine and transported to tissues like the liver, where it is converted to its coenzyme forms. Deficiency can cause cheilosis, glossitis and corneal vascularization. Rich dietary sources include milk, meat, eggs and liver.
Mechanism of action of Chymotrypsin & Lysozyme.pptxVanshikaVarshney5
Chymotrypsin and Lysozyme are the most important enzymes. Mechanism of action of these enzymes and introduction of these enzyme are given in this presentation in simple, easy and understanding language. Hope you will find it useful :)
Introduction to nucleic acid, chemistry of nucleotiides , july 2020enamifat
This document provides an introduction to nucleic acids, their components, and chemistry. It can be summarized as follows:
1. Nucleic acids are high molecular weight polymers composed of nucleotides linked by phosphodiester bonds. The nucleotides contain a pentose sugar, phosphate group, and nitrogenous base.
2. DNA contains deoxyribose and RNA contains ribose. The nitrogenous bases in DNA are adenine, guanine, cytosine, and thymine, while in RNA thymine is replaced by uracil.
3. Nucleotides are the monomers that make up nucleic acids. They contain a nucleoside (pentose sugar + nitrogenous base), and one or more phosphate groups
Absorption, transport and metabolism of pyridoxineDomina Petric
The document summarizes the absorption, transport, and metabolism of pyridoxine (vitamin B6). It states that pyridoxine is absorbed via passive diffusion in the small intestine and transported to the liver bound to albumin in plasma. In tissues and liver, it is phosphorylated to its active form, pyridoxal phosphate. The liver plays a central role in interconverting pyridoxine forms and metabolizing excess amounts, which are ultimately excreted in urine as 4-pyridoxic acid. Alcohol and the drug INH can interfere with pyridoxine metabolism by displacing or binding to pyridoxal phosphate.
Absorption, transport and metabolism of niacinDomina Petric
The document summarizes the absorption, transport, and metabolism of niacin. It discusses how niacin is absorbed in the small intestine through facilitated diffusion. It is transported through the bloodstream as nicotinic acid and nicotinamide and taken up by tissues. Within tissues, it is converted to its coenzyme forms NAD and NADP which are used to drive cellular processes. The document also outlines how humans can synthesize niacin from the amino acid tryptophan through multiple enzymatic steps in the liver.
1) The document discusses coenzymes and vitamins, which are organic molecules that serve as cofactors for enzyme reactions in the body. It covers various coenzymes like ATP, NAD+, FAD, and FMN that are derived from vitamins and transport chemical groups between enzymes.
2) Vitamins are classified as either water-soluble or fat-soluble. Water-soluble vitamins include the B vitamins and vitamin C, while fat-soluble vitamins are vitamins A, D, E, and K. Vitamins function as coenzymes and are essential for growth, development and various metabolic processes.
3) The document provides details on the structures and functions of important coen
Ionophores are molecules that transport ions across biological membranes. They contain both hydrophilic regions that bind ions and hydrophobic regions that interact with membrane lipids. Ionophores are classified based on their mechanism of action as either mobile carrier ionophores which transport ion complexes, or channel-forming ionophores which introduce pores for ion passage. Examples include valinomycin which transports potassium ions, gramicidin A which forms channels for cation transport, and ionomycin which carries calcium ions into cells. Ionophores have important applications as antibiotics, in research to manipulate cellular physiology, and as feed additives to improve livestock growth and productivity.
Significance of shine dalgarno sequencePrajaktaPanda
The shine dalgarno sequence is a ribosomal site in the prokaryotic bacterial mRNA which helps in protein synthesis by aligning the ribosome with the start codon. It's significance deals with it's effect and importance during the translation process within an mRNA.
This document summarizes the isolation of pigments from hibiscus flowers. It describes pigments as water- and oil-insoluble natural or synthetic compounds that impart color. The main pigment in hibiscus is anthocyanin, specifically cyanidin 3-sophoroside. The extraction method involves soaking hibiscus petals in methanol or ethanol with either hydrochloric acid or citric acid. Citric acid extraction yields a larger amount and is more recommended. Nuclear magnetic resonance spectroscopy was used to analyze the extracted pigment. Anthocyanins have various health benefits and are used as food colors, dyes, and pH indicators.
Riboflavin,flavoproteins and their clinical applicationsrohini sane
A presentation in lucid-style on Riboflavin (vitamin B2), Flavoproteins and their clinical applications for MBBS , BDS , B Pham and Biotechnology students to facilitate easy leaning.
Pyridoxine, also known as vitamin B6, is a water-soluble vitamin that exists in three forms - pyridoxine, pyridoxal, and pyridoxamine. All three forms are precursors to pyridoxal phosphate (PLP), the active coenzyme form of vitamin B6. PLP acts as a cofactor in many enzymatic reactions involved in amino acid, lipid, and carbohydrate metabolism. Deficiency of vitamin B6 can result in neurological, dermatological, and hematological symptoms due to its role in many biochemical pathways.
This document provides an overview of protein structure and function. It discusses the four levels of protein structure from primary to quaternary structure. The 20 standard amino acids that serve as building blocks of proteins are described in terms of their chemical and physical properties. Amino acids contain both polar and non-polar side chains that influence protein structure and function. Key concepts covered include protein folding, the zwitterionic nature of amino acids, and interactions like disulfide bonds and hydrophobic effects that stabilize protein tertiary structure.
Post translational modification of protienkamilKhan63
The document discusses post-translational modifications (PTM) of proteins. It defines PTM as the chemical modification of proteins after translation, including phosphorylation, acetylation, methylation, glycocylation, and other types of modifications. These modifications are important as they increase protein diversity and regulate functions like activity, localization, and interactions. The document also describes techniques for detecting PTM, including mass spectrometry and blotting.
Vitamins are organic compounds that are required by humans in small amounts. They are classified as either water-soluble or fat-soluble. Water-soluble vitamins include the B vitamins and vitamin C, while fat-soluble vitamins are vitamins A, D, E, and K. Vitamins function as coenzymes and play important roles in metabolism, energy production, blood cell formation, nerve transmission, gene expression, and other processes. Deficiencies can result in conditions like beriberi, pellagra, scurvy, and rickets.
Vitamins are organic compounds that are required in small amounts to maintain normal health. The word "vitamin" comes from the Latin word "vita" meaning life. There are two types of vitamins - fat soluble (A, D, E, K) and water soluble (B complex, C). Vitamin A is important for vision, epithelial cell integrity, reproduction, resistance to infection, and bone remodeling. Symptoms of vitamin A deficiency include night blindness, dry eyes, and corneal damage.
This document summarizes key information about vitamins. It defines vitamins as organic molecules that serve essential functions in the body as cofactors for enzymatic reactions, though they are required in small amounts and must be obtained through diet. The document outlines the two types of vitamins - fat soluble (A, D, E, K) and water soluble (B complex, C) - and provides details on individual vitamins, including their roles, deficiency symptoms, dietary sources, and recommended daily values.
Vitamins and minerals are essential micronutrients that serve important functions in the body. Vitamins are organic compounds needed in small amounts that can be divided into water and fat soluble categories. Minerals are inorganic elements that are classified as major or trace minerals needed for processes like bone and tissue formation. The document provides details on the functions, sources, and deficiency effects of important vitamins like A, D, C and B vitamins and minerals like calcium, iron, and iodine. Fortification of foods can help address micronutrient deficiencies in populations.
Vitamins are chemical compounds that regulate human body functions and metabolism. They are necessary for converting food into energy and tissues. There are several types of vitamins including A, B1, B2, B12, C, D, E, and K. Each vitamin plays an important role and deficiency can cause specific health issues. For example, vitamin A deficiency can cause night blindness, while vitamin C deficiency results in scurvy. The document provides information on each vitamin's function, deficiency symptoms, and major food sources.
This is a simple powerpoint presentation about vitamins. Done by year 1 medical students at the University of Science and Technology, Sana'a, Republic of Yemen. (2007/2008)
This document provides an overview of vitamins, including their categories, food sources, and functions. It discusses water soluble vitamins like thiamine, riboflavin, niacin, pyridoxine, folate, cobalamin, biotin, pantothenic acid, and choline. It also covers fat soluble vitamins A, D, E, and K. For each vitamin, it lists its functions, recommended daily intake, and deficiency symptoms.
People with increased nutrient needs may require supplements. Women who menstruate heavily may need iron supplements. Newborns require a single vitamin K dose at birth. Women of childbearing age need folic acid supplements to reduce neural tube defects in babies. Vitamins are organic compounds essential in small amounts and must be obtained from food or supplements. They regulate chemical reactions and cannot be synthesized by the body. Fat soluble vitamins like A, D, E and K are stored in tissues while water soluble vitamins like the B vitamins and C are not stored and require constant supply.
This document provides an overview of water soluble vitamins, including their classification, recommended daily allowances, sources, properties, and individual details. It discusses 8 water soluble vitamins - thiamine, riboflavin, niacin, pyridoxine, vitamin C, folic acid, and vitamin B12. For each vitamin, it describes structure, active forms, biochemical functions, causes of deficiency, and deficiency manifestations. The document is intended as an educational reference on essential water soluble vitamins and their roles in human nutrition and health.
This document discusses vitamins, minerals, and water. It explains that vitamins and minerals regulate body functions and must be ingested daily through foods like fruits, vegetables, and whole grains. Dark leafy greens contain the most vitamins and minerals. Water carries nutrients through the body, regulates temperature, and prevents dehydration. We should drink half our body weight in ounces of water daily. The document then provides details on specific vitamins (A, D, E, K, C, B vitamins) and minerals, their functions, sources, deficiencies, and toxicities.
The document discusses vitamins, which are divided into fat-soluble and water-soluble categories. It provides details on individual vitamins including their names, functions, deficiency symptoms, recommended daily allowances and food sources. Key vitamins covered include A, D, E, K, B-complex vitamins, vitamin C. It also discusses vitamin supplements and controversies around their use.
Vitamins and Anemia:
Your body needs vitamins ( nutrients found in most foods) for many reasons, including producing healthy red blood cells. If your body is deficient in certain key vitamins, you can develop a type of anemia ( a condition in which your blood is low on healthy red blood cells ) called vitamin deficiency anemia.
Red blood cells carry oxygen from your lungs to all parts of your body. Without enough healthy red blood cells, your body can't get the oxygen it needs to feel energized. To produce red blood cells, your body needs iron and certain vitamins along with adequate protein and calorie intake.
Vitamin deficiency anemia can also lead to other health problems. Fortunately, you can usually correct vitamin deficiency anemia with supplements and dietary changes.
Glycolysis is the metabolic pathway that breaks down glucose to produce energy in the form of ATP. It occurs in two phases, with the first phase priming the pathway by producing intermediate molecules and consuming 2 ATP per glucose. The second phase yields a net production of 2 ATP per glucose by oxidizing intermediate molecules and harnessing the energy to phosphorylate ADP to ATP. A key regulatory step is the initial phosphorylation of glucose to glucose-6-phosphate by hexokinase, which traps glucose inside cells. Glycolysis is versatile in that it can function aerobically or anaerobically depending on oxygen availability.
Vitamin K: Tool to control the osteophrosisMuhammad Yasin
Vitamin K plays an important role in bone health by activating proteins involved in bone metabolism. Low vitamin K status is associated with increased risk of osteoporosis and bone fractures. The document discusses the functions of vitamin K, how it relates to osteoporosis, and evidence that vitamin K supplementation can help reduce bone loss and fractures by increasing carboxylation of osteocalcin and other proteins. It concludes that vitamin K should be considered mandatory for controlling osteoporosis.
Bone health relies on getting enough key vitamins and minerals. Calcium and vitamin D help form bones and aid mineralization. They work with phosphorus, magnesium, fluoride, and vitamin K. Calcium strengthens bones while vitamin D facilitates calcium absorption. Vitamin K is needed for blood clotting and bone proteins. Phosphorus and magnesium also contribute to bone structure and health. Getting the right amounts of these nutrients through diet helps promote strong bones and prevents conditions like osteoporosis and rickets.
The Indian Dental Academy is the Leader in continuing dental education , training dentists in all aspects of dentistry and
offering a wide range of dental certified courses in different formats.
The document discusses introducing instruction on herbal medicine into the second year medical school curriculum at West Virginia University. It provides an overview of the need for physician knowledge of herbal remedies, the school's approach, and evaluation of the program's impact. Students reported gaining awareness and knowledge but maintaining a cautious view, and felt exposure to herbal medicine was an important part of their training.
This document summarizes key aspects of several water-soluble vitamins, including their functions, absorption, clinical deficiencies, food sources, and uses for supplementation. It discusses the vitamins thiamine, riboflavin, niacin, pyridoxine, folic acid, and vitamin C, describing how each acts as a coenzyme in important metabolic processes and what health issues can result from deficiencies. Food sources rich in each vitamin are also outlined, as well as therapeutic uses for supplementation.
Biochemistry of Vitamins for Medical Students-RajendraRajendra Dev Bhatt
Vitamins are organic compounds that are essential nutrients required in small amounts for normal physiological functions. They are classified as either fat-soluble (A, D, E, K) or water-soluble (B complex, C). Vitamins act as coenzymes and play important roles in metabolism. Deficiencies can result from inadequate intake or absorption and cause diseases like beriberi (B1), pellagra (B3), and scurvy (C).
This document provides information about various vitamins. It discusses 13 vitamins - thiamine (B1), riboflavin (B2), niacin (B3), pantothenic acid (B5), pyridoxine (B6), biotin (B7), folic acid (B9), cyanocobalamin (B12), ascorbic acid (C), retinol (A), ergocalciferol (D), tocopherol (E), and phylloquinone (K). For each vitamin, it mentions the chemical name, sources, functions, deficiency symptoms, absorption and any relevant chemistry. The document provides details on the roles of these vitamins
The document discusses water soluble vitamins. It notes that ten vitamins including ascorbic acid, bioflavonoids, and the B vitamins are water soluble. These vitamins are not readily stored by the body and deficiencies can occur quickly if not obtained from the diet. The B vitamins, also called the B complex vitamins, are involved in energy production and hematopoiesis. They are mainly found in plant foods like cereal grains, flour, bran and yeast. Exceptions include leafy greens as a good source of folic acid and foods like meat, fish and dairy being good sources of vitamin B12. The document then discusses specific B vitamins like thiamine, riboflavin and their roles in biochemical processes.
The document discusses various B vitamins, including their chemistry, food sources, roles in the body, and deficiency diseases. It provides information on vitamin B1 (thiamine), B2 (riboflavin), B3 (niacin), B5 (pantothenic acid), B6 (pyridoxine), B7 (biotin), and B9 (folic acid). The key points made are that B vitamins serve as cofactors in many enzyme reactions involved in metabolism and must be obtained through the diet as humans cannot synthesize most of them. Deficiencies can result in diseases like beriberi, pellagra, and anemia.
This document provides an overview of the B complex vitamins, focusing on Thiamine (B1). It discusses the chemistry, functions, deficiency symptoms, and recommended daily intake of Thiamine. Key points include: Thiamine acts as a coenzyme (Thiamine Pyrophosphate) and is important for energy metabolism. Deficiency can cause Beriberi, with symptoms of weakness, edema, and nerve damage. Good sources include whole grains, yeast, and the aleurone layer of cereals.
VITAMINS introduction water soluble and fat soluble vitamins.pptxMwambaChikonde1
The document discusses vitamins, including their classification, functions, sources, and deficiencies. It covers both water-soluble vitamins like vitamin C and B vitamins, as well as fat-soluble vitamins A, D, E, and K. Key points include:
- Vitamins are organic compounds required in small amounts that must be obtained from diet. They are classified as either water-soluble or fat-soluble.
- Water-soluble vitamins include vitamin C and B vitamins. Deficiencies can cause conditions like scurvy and beriberi. Fat-soluble vitamins are absorbed with fats and stored in the liver.
- All vitamins function as coenzymes and play important
Vitamins serve as cofactors for enzymatic reactions by binding to enzymes to form active coenzyme complexes. Most B vitamins function as coenzymes involved in energy production and metabolism. Vitamin deficiencies can result in diseases; for example, thiamine deficiency causes beriberi and Wernicke-Korsakoff syndrome. Vitamins are essential for many cellular processes and metabolic reactions in the body.
Vitamins are organic compounds that are essential in small amounts for various biological functions. They are classified as either fat-soluble (A, D, E, K) or water-soluble (B complex, C). Vitamins act as coenzymes and are involved in growth, metabolism, and disease prevention. Deficiencies can cause conditions like scurvy, beriberi, and pellagra. The document discusses the chemistry, absorption, functions, sources, and deficiencies of various vitamins.
This document summarizes key information about vitamins. It defines vitamins and explains that most are not synthesized in the body and must come from diet. Vitamins are divided into two groups: fat-soluble vitamins A, D, E, and K which are stored in the liver, and water-soluble B vitamins and vitamin C which are not stored. The document then provides details on individual B vitamins, including their functions, sources, and deficiency symptoms.
This document provides information on vitamin A, including its:
- Occurrence in foods like fish liver, carrots, and green vegetables
- Deficiency causes night blindness and xerophthalmia if severe
- Role in vision through the visual cycle and rhodopsin regeneration
- Other roles in growth, reproduction, and epithelial cell maintenance
It also discusses the chemistry, isolation, constitution, and biosynthesis of vitamin A.
This document discusses a lecture on vitamins and cofactors. It covers the classification of vitamins as water-soluble or fat-soluble. Vitamins serve as coenzymes and cofactors that facilitate enzyme reactions in the body. Specific vitamins discussed include thiamine (B1), riboflavin (B2), and niacin (B3). The roles and functions of these vitamins as coenzymes in important metabolic pathways like glycolysis and the citric acid cycle are described. Deficiency symptoms for each vitamin are also mentioned.
This document summarizes key information about water soluble vitamins. It discusses Vitamin C (ascorbic acid) in depth, covering its structure, functions, metabolism, deficiency, sources, and more. It also briefly covers the B vitamin complex, including thiamine (B1) and riboflavin (B2). The document provides detailed information on the biological roles and importance of water soluble vitamins.
Vitamins are organic compounds that are required in small amounts for various biological functions. They are classified as either fat-soluble (A, D, E, K) or water-soluble (B complex, C). Fat-soluble vitamins are absorbed with fats and stored in the liver, while water-soluble vitamins dissolve in water, are not stored, and excess is excreted in urine. Deficiencies of vitamins can cause diseases like scurvy, beriberi, and pellagra due to their roles as coenzymes in metabolic processes.
The document discusses water-soluble vitamins. It provides details on the chemical structure, functions, dietary sources, metabolism and deficiencies of several B vitamins (thiamine, riboflavin, niacin, pantothenic acid, vitamin B6, biotin, cobalamin, folic acid) and vitamin C. The key points covered are: the B vitamins function as enzyme cofactors; deficiency of a single B vitamin is rare as poor diets often cause multiple deficiencies; and the water-soluble vitamins must be obtained regularly from the diet as they are not stored in the body for long periods.
6 -water soluble vitamins and exercisesSiham Gritly
The document discusses various water soluble vitamins, their roles in metabolism and energy production, and how exercise can increase the body's needs for certain vitamins like the B vitamins and vitamin C. It focuses on specific vitamins like thiamine, riboflavin, niacin, pantothenic acid, pyridoxine, folic acid, cobalamin, and vitamin C, describing their functions, deficiency symptoms, food sources, and relationships with exercise. The document provides information on vitamins to support nutrition and fitness goals.
This document discusses hyperkalemia, defined as a plasma potassium level above 5.5 mM. Causes include a shift of potassium from cells to plasma due to factors like acidosis, medications, or tissue breakdown. Symptoms range from cardiac arrhythmias to paralysis. Diagnosis involves blood tests. Treatment involves calcium to stabilize the heart, insulin or beta-agonists to shift potassium into cells, and resins, diuretics or dialysis to remove potassium from the body. The document provides details on treatment options, their mechanisms of action, dosages and cautions.
This document defines and describes various types of shock, including their causes, pathophysiology, clinical manifestations, and treatment. It discusses hypovolemic, cardiogenic, septic, and neurogenic shock. Hypovolemic shock results from loss of circulating blood volume from hemorrhage or fluid losses. Cardiogenic shock is caused by severe cardiac dysfunction. Septic shock involves systemic inflammation and hypotension due to infection. Neurogenic shock occurs when there is reduced sympathetic tone affecting blood vessels and the heart. The document provides details on the mechanisms, signs, and goals of treatment for each type of shock.
1. Septicemia, also known as blood poisoning, occurs when large amounts of bacteria enter the bloodstream, potentially leading to septic shock. It is commonly caused by pneumonia or infections associated with medical interventions.
2. Symptoms of sepsis include fever, fast heart rate, fast or difficult breathing, pale or sweaty skin, and changes in mental status. Septic shock occurs when sepsis leads to dangerously low blood pressure and organ dysfunction.
3. Diagnosis involves identifying an infection and signs of organ dysfunction. Laboratory tests show abnormalities in white blood cell count, platelet count, lactate levels, and organ functioning. Empiric antibiotic therapy is started based on suspected infection site before culture results.
Abortion, ectopic pregnancy and gestational trophoblastic diseasesSADDA_HAQ
This document discusses abortion, ectopic pregnancy, and gestational trophoblastic diseases. It defines abortion and describes different types including spontaneous, induced, threatened, inevitable, complete, incomplete, and missed. Common causes of miscarriage in the first and second trimester are outlined. Symptoms, management, and complications of threatened, inevitable, incomplete, missed, and septic abortions are summarized. The document also discusses ectopic pregnancy, describing the sites of implantation including tubal, ovarian, abdominal, and uterine. Symptoms and management of acute, unruptured, and chronic ectopic pregnancies are provided.
This document discusses various types of cell membrane transport. It describes passive transport mechanisms like diffusion and osmosis that move molecules from high to low concentration without ATP. Diffusion can occur through simple diffusion or facilitated diffusion using transport proteins. Osmosis is diffusion of water across a semi-permeable membrane. Active transport uses ATP and transports molecules against their concentration gradient using pumps, phagocytosis, or endocytosis and exocytosis. Various factors like concentration gradients and molecule properties affect the rate of transport. Membrane transport proteins include aquaporins, ion channels, and solute carriers.
Pharmacology is the science of drugs and their interaction with living systems. It has two main divisions: pharmacokinetics, which describes what the body does to drugs, and pharmacodynamics, which describes what drugs do to the body. Key concepts in pharmacology include affinity, intrinsic activity, agonists, and antagonists. Pharmacology underlies the use of drugs in disease prevention and treatment.
This document discusses various routes of drug administration including oral, sublingual, rectal, parenteral, and others. The oral route is the most common as it is convenient, cheap, and painless. However, some drugs are destroyed by gastric juices or have extensive first pass metabolism. Alternative routes like sublingual can provide rapid absorption while avoiding first pass effects. Parenteral routes like intravenous provide the fastest onset but require more expertise to administer. The route chosen depends on drug properties and patient factors.
This document discusses drug nomenclature and the different names used to identify drugs. It explains that drugs have three main names: the chemical name, non-proprietary name, and proprietary name. The chemical name identifies the precise molecular structure and is used by chemists. The non-proprietary name is a short, concise name given by bodies like the USAN and is used more broadly. Proprietary names are given by pharmaceutical companies to brand their versions of drugs. Examples provided are paracetamol, which has the chemical name N-(4-hydroxyphenyl)acetamide and proprietary brand names like Calpol, and aspirin, with the chemical name acetyl salicylic acid and brand name Dispirin
This document defines endogenous and exogenous pigments. Endogenous pigments are either normal constituents of cells or accumulate under special circumstances, such as melanin, ochronosis, and haemoprotein-derived pigments like haemosiderin, bilirubin, and porphyrins. Exogenous pigments come from outside the body through inhalation, ingestion, or injection. Inhaled pigments can cause occupational lung diseases, ingested pigments can lead to argyria or lead poisoning, and injected pigments like tattoo ink remain permanently in the dermis.
Pathological calcification involves the abnormal deposition of calcium salts in tissues other than bone. There are two main types: dystrophic calcification occurs in dead or damaged tissue with normal calcium levels, while metastatic calcification affects normal tissues and results from disorders that increase calcium levels in the blood (hypercalcemia). Dystrophic calcification is seen in areas of necrosis, atherosclerotic plaques, and infarcts. Metastatic calcification commonly involves the kidneys, lungs, blood vessels, and stomach, and is caused by hyperparathyroidism, bone destruction, or excessive vitamin D intake.
Sulfuric acid poisoning causes painful burning in the throat, vomiting, constipation, and suppressed urination. Signs include chalky white teeth, erosion of skin and mucous membranes around the mouth, and blackening of areas exposed to the acid. A fatal dose is 5-10 ml, with death occurring within 12-18 hours by shock, stomach perforation, or peritonitis. Treatment involves dilution of the acid with milk or water followed by antacids and corticosteroids. Post-mortem findings include erosion of skin around the mouth, corrosion of the trachea and larynx, and blackened charring of the stomach. Sulfuric acid poisoning can be accidental, suicidal, or
Sterilization is a process that makes a person sterile without affecting their potency or sexual functions. There are different types of sterilization including compulsory sterilization ordered by the state for mentally defective people or sexual criminals, voluntary sterilization with consent from married couples, and therapeutic or eugenic sterilization. Sterilization can also be used as a contraceptive method.
The document summarizes information about the Bhilawan nut (Semecarpus anacardium). It describes the tree as a moderate sized deciduous tree native to India. The nuts are approximately 1 inch long, black when ripe, and contain a bitter irritant juice. Contact with the juice can cause artificial bruises, blisters, ulcers, and skin irritation or sloughing. Ingestion of the nuts may lead to severe gastrointestinal, respiratory, circulatory and central nervous system effects, and can potentially be fatal in doses of 5-10 grams within 12-24 hours. Treatment involves gastric lavage and washing exposed skin with antiseptic water. The nuts have been used criminally to induce abort
This document discusses metal toxicity and provides information about three heavy metals - arsenic, lead, and mercury. It notes that arsenic, lead, and mercury are numbers 1, 2, and 3, respectively, on the ATSDR's "Top 20 List" of hazardous substances. For each metal, it outlines common sources of exposure, symptoms of poisoning, and other key facts. The document emphasizes that heavy metals can accumulate in the body over time and cause both acute and chronic toxicity. It also stresses the importance of preventing environmental pollution to avoid health issues.
This document discusses irreversible cell injury and the different types of cell death. It begins by introducing the concept that all organ injuries start with alterations at the cellular level. There are two main classes of cell injury - irreversible cell injury, which leads to cell death through necrosis or apoptosis, and reversible cell injury, where cells can survive if the stressor is removed and damage is mild. The two main causes of irreversible injury are an inability to reverse mitochondrial dysfunction and profound disturbances in cell membrane function. Necrosis and apoptosis are then described in more detail, including their morphological changes, types, and differences between the two forms of cell death.
Dhatura is a poisonous plant belonging to the Solanaceae family. All parts of the plant, including the fruit, flowers, and seeds, contain toxic belladonna alkaloids like hyoscine, hyoscyamine, and atropine. Ingesting the seeds or plant parts can cause anticholinergic effects like a dry mouth, blurred vision, delirium, and respiratory depression. The fatal dose is 75-125 seeds, which can cause death within 24 hours due to respiratory failure. Treatment involves gastric lavage, activated charcoal, physostigmine as an antidote, and supportive care. Forensically, Dhatura poisoning may be identified by the presence of undig
ABDOMINAL TRAUMA in pediatrics part one.drhasanrajab
Abdominal trauma in pediatrics refers to injuries or damage to the abdominal organs in children. It can occur due to various causes such as falls, motor vehicle accidents, sports-related injuries, and physical abuse. Children are more vulnerable to abdominal trauma due to their unique anatomical and physiological characteristics. Signs and symptoms include abdominal pain, tenderness, distension, vomiting, and signs of shock. Diagnosis involves physical examination, imaging studies, and laboratory tests. Management depends on the severity and may involve conservative treatment or surgical intervention. Prevention is crucial in reducing the incidence of abdominal trauma in children.
Promoting Wellbeing - Applied Social Psychology - Psychology SuperNotesPsychoTech Services
A proprietary approach developed by bringing together the best of learning theories from Psychology, design principles from the world of visualization, and pedagogical methods from over a decade of training experience, that enables you to: Learn better, faster!
share - Lions, tigers, AI and health misinformation, oh my!.pptxTina Purnat
• Pitfalls and pivots needed to use AI effectively in public health
• Evidence-based strategies to address health misinformation effectively
• Building trust with communities online and offline
• Equipping health professionals to address questions, concerns and health misinformation
• Assessing risk and mitigating harm from adverse health narratives in communities, health workforce and health system
Rasamanikya is a excellent preparation in the field of Rasashastra, it is used in various Kushtha Roga, Shwasa, Vicharchika, Bhagandara, Vatarakta, and Phiranga Roga. In this article Preparation& Comparative analytical profile for both Formulationon i.e Rasamanikya prepared by Kushmanda swarasa & Churnodhaka Shodita Haratala. The study aims to provide insights into the comparative efficacy and analytical aspects of these formulations for enhanced therapeutic outcomes.
Does Over-Masturbation Contribute to Chronic Prostatitis.pptxwalterHu5
In some case, your chronic prostatitis may be related to over-masturbation. Generally, natural medicine Diuretic and Anti-inflammatory Pill can help mee get a cure.
Local Advanced Lung Cancer: Artificial Intelligence, Synergetics, Complex Sys...Oleg Kshivets
Overall life span (LS) was 1671.7±1721.6 days and cumulative 5YS reached 62.4%, 10 years – 50.4%, 20 years – 44.6%. 94 LCP lived more than 5 years without cancer (LS=2958.6±1723.6 days), 22 – more than 10 years (LS=5571±1841.8 days). 67 LCP died because of LC (LS=471.9±344 days). AT significantly improved 5YS (68% vs. 53.7%) (P=0.028 by log-rank test). Cox modeling displayed that 5YS of LCP significantly depended on: N0-N12, T3-4, blood cell circuit, cell ratio factors (ratio between cancer cells-CC and blood cells subpopulations), LC cell dynamics, recalcification time, heparin tolerance, prothrombin index, protein, AT, procedure type (P=0.000-0.031). Neural networks, genetic algorithm selection and bootstrap simulation revealed relationships between 5YS and N0-12 (rank=1), thrombocytes/CC (rank=2), segmented neutrophils/CC (3), eosinophils/CC (4), erythrocytes/CC (5), healthy cells/CC (6), lymphocytes/CC (7), stick neutrophils/CC (8), leucocytes/CC (9), monocytes/CC (10). Correct prediction of 5YS was 100% by neural networks computing (error=0.000; area under ROC curve=1.0).
Osteoporosis - Definition , Evaluation and Management .pdfJim Jacob Roy
Osteoporosis is an increasing cause of morbidity among the elderly.
In this document , a brief outline of osteoporosis is given , including the risk factors of osteoporosis fractures , the indications for testing bone mineral density and the management of osteoporosis
Cell Therapy Expansion and Challenges in Autoimmune DiseaseHealth Advances
There is increasing confidence that cell therapies will soon play a role in the treatment of autoimmune disorders, but the extent of this impact remains to be seen. Early readouts on autologous CAR-Ts in lupus are encouraging, but manufacturing and cost limitations are likely to restrict access to highly refractory patients. Allogeneic CAR-Ts have the potential to broaden access to earlier lines of treatment due to their inherent cost benefits, however they will need to demonstrate comparable or improved efficacy to established modalities.
In addition to infrastructure and capacity constraints, CAR-Ts face a very different risk-benefit dynamic in autoimmune compared to oncology, highlighting the need for tolerable therapies with low adverse event risk. CAR-NK and Treg-based therapies are also being developed in certain autoimmune disorders and may demonstrate favorable safety profiles. Several novel non-cell therapies such as bispecific antibodies, nanobodies, and RNAi drugs, may also offer future alternative competitive solutions with variable value propositions.
Widespread adoption of cell therapies will not only require strong efficacy and safety data, but also adapted pricing and access strategies. At oncology-based price points, CAR-Ts are unlikely to achieve broad market access in autoimmune disorders, with eligible patient populations that are potentially orders of magnitude greater than the number of currently addressable cancer patients. Developers have made strides towards reducing cell therapy COGS while improving manufacturing efficiency, but payors will inevitably restrict access until more sustainable pricing is achieved.
Despite these headwinds, industry leaders and investors remain confident that cell therapies are poised to address significant unmet need in patients suffering from autoimmune disorders. However, the extent of this impact on the treatment landscape remains to be seen, as the industry rapidly approaches an inflection point.
Basavarajeeyam is a Sreshta Sangraha grantha (Compiled book ), written by Neelkanta kotturu Basavaraja Virachita. It contains 25 Prakaranas, First 24 Chapters related to Rogas& 25th to Rasadravyas.
2. Vitamins are a heterogeneous group of organic
compounds which are essential for animals and
human beings
They are required in very minute quantities
They do not provide energy but their dietary
intake is essential as they perform biochemical
functions essential for normal health, growth and
reproduction
3. Deficiencies of vitamins produce specific
diseases which can be prevented or cured by
administration of the pure vitamins or intake of
natural foods containing the concerned vitamins
Several deficiency diseases and, in some
instances, their treatments were discovered long
before the discovery of the vitamins themselves
4. Scurvy and beriberi are examples of diseases
which could be cured by giving citrus fruits and rice
polishings respectively even though the causes of
these diseases were not known
Since the chemical natures of vitamins were not
known at the time of their discovery, they were
named after the letters of the alphabet
These names have now been largely replaced by
chemical names
5. Vitamins can be classified into two groups on the
basis of their solubility:
1.Water-soluble vitamins
2.Fat-soluble vitamins
6. Water-soluble Vitamins
These are soluble in water, and are not stored in
the body
Their excessive intake is wasteful but does not
cause any toxicity
The water-soluble vitamins essential for human
beings are vitamins of the B-complex family
(thiamin, riboflavin, niacin, pantothenic acid,
pyridoxine, biotin, lipoic acid, folic acid and
cobalamin) and vitamin C
7. Fat-soluble Vitamins
These are soluble in fat but insoluble in water,
and include vitamins A, D, E and K
They are present in food in association with
dietary lipids
They can be stored in the body and their
excessive intake can be toxic
8. WATER-SOLUBLE VITAMINS
Since the water-soluble vitamins are not stored in
the body to any appreciable extent, they have to
be taken in the diet every day
Losses can occur during cooking as some of
them are heat-labile
9. Some of the water-soluble vitamins are
synthesised by intestinal bacteria
If intestinal bacteria are destroyed, for example
by anti-biotic therapy, additional intake is
required
10. THIAMIN
Thiamin (vitamin B1) is heat-stable in acidic
medium but not in basic medium
It is oxidised by mild oxidising agents to
thiochrome which is biologically inactive
Chemically, it is made up of a substituted
pyrimidine linked through a methylene bridge to
substituted thiazole
11. N H 2
|
C
C H 3
|
C
H C — C3 C H
N C — C H — N2
+
C — C H — C H — O H2 2
N
C H S
2 ,5 - D im e th y l-
4 - a m in o p y r im id in e
4 - M e th y l- 5 - h y d r o x y -
e th y lth ia z o le
T h ia m in
12. Functions
Thiamin forms a coenzyme, thiamin
pyrophosphate (TPP) or thiamin diphosphate (TDP)
N H 2
|
C
C H 3
|
C
H C — C3 C H
N C — C H — N2
+
C — C H — C H — O — P — O — P — O H2 2
N
C H S
O
||
O
||
|
O H
|
O H
13. TPP is a coenzyme for transketolase (in HMP
shunt) and α-keto acid dehydrogenases e.g. pyruvate
dehydrogenase, α-ketoglutarate dehydrogenase and
branched-chain α-keto acid dehydrogenase
Impaired activity of α-keto acid dehydrogenases
due to thiamin deficiency can limit the availability
of energy
14.
15. Sources
Whole grain cereals, pulses, nuts, yeast, liver,
kidney, heart and meat are good sources of
thiamin
In cereals, thiamin is present mainly in the outer
layer of the grain
Removal of the outer layer, e.g. by milling, causes
considerable loss of thiamin
16. Requirement
The recommended daily allowance (RDA) for
thiamin is 0.5 mg/1,000 kcal of energy or 1 - 1.5
mg/day in adults
The requirement increases in alcoholics and in
hypermetabolic states e.g. pregnancy, fever,
hyperthyroidism etc
17. Deficiency
People consuming polished rice or refined wheat
flour as their staple food are susceptible to
thiamin deficiency due to removal of the outer layer of
the grain
Parboiling of rice decreases the loss of thiamin
During parboiling, paddy is soaked in warm water
for a few hours and, then, steam-dried
18. Thiamin percolates into the deeper part of the
grain
Polishing of parboiled rice leads to a limited loss
of thiamin
Alcoholics can develop deficiency as alcohol
impairs the absorption of thiamin and its
conversion into TPP (Wernicke-Korsakoff
syndrome)
19. Deficiency of thiamin causes beriberi which
affects:
1. Central nervous system
2. Cardiovascular system
3. Gastrointestinal tract
20. Central nervous system
Thiamin deficiency causes peripheral neuritis
involving sensory as well as motor nerves
Sensory involvement leads to hyperaesthesia,
numbness, tingling and pain
Motor involvement leads to muscular weakness,
sluggish reflexes, ataxia and paralysis
21. Cardiovascular system
The heart muscle becomes weak resulting in
congestive heart failure
This, in turn, causes oedema and ascites
23. Predominant involvement of central nervous
system is known as dry beriberi as there is no
collection of water in interstitial tissue in this
condition
Predominant involvement of cardiovascular
system is known as wet beriberi because of the
occurrence of oedema
Mixed beriberi is more common in which different
systems are involved in varying degrees
24. Diagnosis of beriberi can be confirmed by some
laboratory investigations
Concentration of pyruvic acid in blood is
increased in beriberi
Thiamin concentration in erythrocytes is
decreased
Urinary thiamin excretion after a test dose is
decreased
25. In normal subjects, most of the test dose is
promptly excreted in urine
Subjects deficient in thiamin retain most of the
test dose in tissues and excrete less in urine
Measurement of transketolase activity in
erythrocytes can confirm the diagnosis
26. RIBOFLAVIN
Riboflavin (vitamin B2) is heat-stable in neutral
and acidic medium but not in basic medium
Its aqueous solution is unstable in sunlight and
ultraviolet light
Riboflavin can be readily reduced to
leucoriboflavin
27. Chemically, riboflavin is 6,7-dimethyl-9-D-ribityl
isoalloxazine
H C —3
||
1
2
45
6
7
8 9
1 0
C H — C — C — C — C H O H2 2
H
|
O H
|
O H
|
O H
|
H
|
H
|
3
NN
N
H C —3 O
N H
O
28. Functions
Riboflavin is a constituent of flavin
mononucleotide (FMN) and flavin adenine
dinucleotide (FAD)
These two coenzymes can undergo reversible
oxidation and reduction, and participate in a
number of oxidation-reduction reactions
The riboflavin portion of FMN and FAD can
reversibly combine with two hydrogen atoms
29. H C —3
||
C H — C — C — C — C H — O — P — O H2 2
H
|
O H
|
O H
|
O H
|
H
|
H
|
NN
N
H C —3 O
N H
O
| |
|
O H
H C —3
||H
NN
N
H
H C —3 O
N H
F M N H 2
F M N
A H 2
A
C H — C — C — C — C H — O — P — O H2 2
H
|
O H
|
O H
|
O H
|
H
|
H
|
O
| |
|
O H
O
O
30. FMN is a:
• constituent of respiratory chain (electron
transfer chain)
• constituent of microsomal hydroxylase system
• coenzyme for L-amino acid oxidase
31. HC—3
||
NN
N
HC—3 O
NH
FAD
HC—3
||
N
HC—3 O
NH
H
N
N
H
AH 2
A
N
N
N
N
NH 2
NH 2
H
|
OH
|
OH
|
OH
|
OH
|
H
|
H
|
O
||
CH— C — C — C — CH— O — P — O — P — O — CH222
OH
|
O
||
|
|
N
N
OH
OH
H
H
OH
OH
H
H
H
H
H
H
O
O
N
N
FADH 2
H
|
OH
|
OH
|
OH
|
OH
|
H
|
H
|
O
||
CH— C — C — C — CH— O — P — O — P — O — CH222
OH
|
O
||
O
O
32. FAD is a coenzyme for several enzymes e.g. D-
amino acid oxidase, acyl CoA dehydrogenase,
succinate dehydrogenase, glycerol-3-phosphate
dehydrogenase, xanthine oxidase, sphingosine
reductase, pyruvate dehydrogenase, α-
ketoglutarate dehydrogenase etc
It is also a constituent of respiratory chain
and microsomal hydroxylase system
35. Deficiency
An isolated deficiency of riboflavin is rare
It is generally combined with other deficiencies
36. Clinical features of riboflavin deficiency include :
• Angular stomatitis (fissures at the angles of
mouth)
• Cheilosis (cracked and swollen lips)
• Glossitis (swollen, painful and magenta-
coloured tongue)
• Seborrheic dermatitis (rough and scaly skin)
• Corneal vascularisation (growth of blood
vessels into the cornea)
37. Laboratory diagnosis of riboflavin deficiency is
difficult
Serum and urinary riboflavin are low in severe
deficiency
Erythrocyte riboflavin is decreased
The urinary excretion of riboflavin after a test
dose is decreased
38. NIACIN
Niacin was known in the past as anti-pellagra
factor, pellagra-preventing factor and vitamin B3
It occurs in two forms, niacin (nicotinic acid) and
niacinamide (nicotinamide)
Both the forms are equally active
Niacin is converted into niacinamide in the body
39. N N
— C O O H — C O N H 2
N ia c i n
( n i c o t i n ic a c i d )
N ia c i n a m i d e
( n i c o t i n a m id e )
40. Functions
Niacin performs its functions in the form of two
coenzymes, nicotinamide adenine dinucleotide
(NAD) and nicotinamide adenine dinucleotide
phosphate (NADP)
Nicotinamide combines with ribose and
phosphoric acid to form a nucleotide, which is linked
with an adenine nucleotide
41. — C O N H 2
C H — O — P — O — P — O — C H2 2
NN
+
N
N H 2
|
N
O H *O H
HH
O HO H
HH
H H HH
OO
N
O H
|
O H
|
O O
|| ||
N A D ( in N A D P , — O H * is e s te r ifie d w ith p h o s p h o r ic a c id )
42. These two coenzymes can undergo reversible
oxidation and reduction, and can act as
coenzymes for several oxidoreductases
C H C HC — C O N H 2 C — C O N H 2
N
|
R
+
N
|
R
C H C HC H C H
C H C H 2
N A D (o r N A D P )
+ +
N A D H (o r N A D P H )
A H 2 A
+ H
+
43. NAD and NADP act as coenzymes in many
metabolic pathways e.g.
•Glycolysis
•Hexose monophosphate shunt
•Citric acid cycle
•Synthesis of fatty acids and steroids
•Oxidation of fatty acids
•Oxidative deamination of amino acids
Generally, NAD acts as coenzyme in catabolic
pathways and NADP in anabolic pathways
44. Some examples of enzymes which require NAD
as a coenzyme are glyceraldehyde-3-
phosphate dehydrogenase, lactate dehydro- genase,
pyruvate dehydrogenase, isocitrate
dehydrogenase, α-ketoglutarate dehydrogenase,
malate dehydrogenase, β-hydroxyacyl CoA
dehydrogenase, glutamate dehydrogenase, IMP
dehydrogenase etc
NAD is also a constituent of the respiratory chain
45. Examples of enzymes requiring NADP as a
coenzyme include glucose-6-phosphate
dehydrogenase, 6-phosphogluconate
dehydrogenase, β-ketoacyl CoA reductase, α,β-
unsaturated acyl CoA reductase, β-hydroxy-β- methyl
glutaryl CoA (HMG CoA) reductase, squalene
synthetase, cholesterol 7-α- hydroxylase, thioredoxin
reductase, haem oxygenase etc
46. Sources
Milk, eggs, meat, liver, yeast, tomatoes and
green leafy vegetables are good sources of niacin
Niacin is also synthesised in human beings from
tryptophan
It has been shown that 1 mg of niacin is
synthesised from 60 mg of tryptophan
47. Vitamin B6 is required in the synthetic pathway as
a coenzyme (pyridoxal phosphate)
Excess of leucine inhibits the conversion of
tryptophan into niacin
49. Deficiency
Deficiency of niacin causes pellagra which is
characterised by stomatitis, glossitis, diarrhoea,
dermatitis and dementia (mental degeneration)
Dermatitis usually affects the exposed parts of the
body
If untreated, the disease can be fatal
50. Pellagra is common in people consuming maize
and sorghum (jowar) as their staple foods
These two are poor in niacin and tryptophan, and
rich in leucine
51. PANTOTHENIC ACID
Pantothenic acid, known in the past as vitamin
B5, is heat-stable in neutral medium but not in
acidic or basic medium
It is not destroyed by oxidising or reducing
agents
It is made up of pantoic acid and β-alanine
52. C H — C — C H — C — N — C H — C H — C O O H2 2 2
C H 3
|
H
|
O
||
C H 3
| ||
O HO H
P a n to ic a c id β - A la n in e
P a n to th e n ic a c id
53. Functions
Pantothenic acid performs its biochemical
functions as a constituent of coenzyme A (CoA)
and acyl carrier protein (ACP)
Both these compounds contain pantothenic acid
in the form of 4´-phosphopantetheine
54. Pantothenic acid is first phosphorylated, by ATP,
at C4 of the pantoic acid residue to form 4´-
phosphopantothenic acid which, then, combines
with cysteine to form 4´-phosphopantothenyl cysteine
C H — C — C H — C — N — C H — C H —2 2 2 C — N — C H — C H — S H2
C H 3
|
H
|
H
|
C O O H
|
O
||
O
||
C H 3
| ||
O HO
|
O = P — O H
4 ´-P h o s p h o p a n to th e n ic a c id C y s te in e
4 ´-P h o s p h o p a n to th e n y l c y s te in e
|
O H
55. 4´-Phosphopantetheine is formed by decarboxy-
lation of the cysteine residue which, after
decarboxylation, is converted into a
thioethanolamine residue
CH—C—CH—C—N—CH—CH—COOH2 2 2
CH3
|
H
|
O
||
CH3
| ||
OHOH
Pantoic acid β-Alanine
Pantothenic acid
56. 4´-Phosphopantetheine is linked with AMP
(provided by hydrolysis of ATP) to form
dephosphocoenzyme A
The ribose moiety of dephosphocoenzyme A is
phosphorylated at C3 to form coenzyme A
58. N
N
N H 2
|
N
O H
H
O
H
H
N
O = P — O H
H
|
|
C H 2
|
O = P — O H
C H C C H C N C H C H2 2 2— — — — — — — C — N — C H — C H — S H2 2
C H 3
|
H
|
H
|
O
| |
O
| |
C H 3
| |
O H
|
O
|
|
O
O
|
O = P — O H
|
O
Coenzyme A
59. In acyl carrier protein, 4´-phosphopantetheine is
esterified with a serine residue of the protein
The –SH group of 4´-phosphopantetheine
remains free
60. Role of coenzyme A
Coenzyme A is also represented as CoA-SH as
its terminal –SH group binds various compounds
This coenzyme participates in a variety of
reactions in the metabolism of carbohydrates, lipids
and amino acids
61. Some examples of such reactions are:
• Oxidative decarboxylation of α-keto acids
• Activation of fatty acids
• Activation of some amino acids
62. Oxidative decarboxylation of α-keto acids
A number of coenzymes are required in this
reaction including CoA
The overall reaction is:
R — C — C O O H + C o A — S H + N A D +
R — C ~ S — C o A + N A D H + H + C O+ +
2
α - K e t o a c i d
A c y l C o A
O
| |
O
| |
63. Pyruvate is converted into acetyl CoA by this
reaction
α-Ketoglutarate is converted into succinyl CoA
64. Activation of fatty acids
Before fatty acids can take part in any reaction,
they have to be converted into their CoA
derivatives
This reaction, known as activation of fatty acids,
is catalysed by acyl CoA synthetase (thiokinase)
65. Subjects deficient in thiamin retain most of the
test dose in tissues and excrete less in urine
Measurement of transketolase activity in
erythrocytes can confirm the diagnosis
R — C H — C O O H + C o A — S H + A T P2
R — C H — C ~ S — C o A + A M P + P P i2
F a t t y a c id
A c y l C o A
O
| |
66. Activation of amino acids
Some amino acids, e.g. leucine, isoleucine and
valine, are converted into their CoA derivatives
before they can be metabolised
67. An important role of CoA is to provide active
acetate (acetyl CoA) for various reactions e.g.
synthesis of fatty acids, cholesterol, ketone bodies,
acetylcholine etc
Active succinate (succinyl CoA) is required for
haem synthesis and for gluconeogenesis from
some amino acids
68. Role of acyl carrier protein
Acyl carrier protein is a constituent of the
multienzyme complex which catalyses de novo
synthesis of fatty acids
69. Sources
Pantothenic acid is widely distributed in animal
and plant foods
It is also synthesised by intestinal bacteria
Liver, kidney, meat, eggs, yeast, wheat, peas
and sweet potatoes are good sources of
pantothenic acid
70. Requirement
The recommended daily intake is 10 mg though a
smaller intake may be sufficient for infants and
children
71. Deficiency
Deficiency of pantothenic acid has not been
reported in human being
In animals, deficiency leads to loss of weight,
loss of hair, greying of hair, anaemia and necrosis
of adrenal glands
Human deficiency can be produced
experimentally, which causes neurological and
gastrointestinal disturbances
72. PYRIDOXINE
Pyridoxine was known in the past as vitamin B6
Vitamin B6 consists of three closely related
pyridine derivatives, pyridoxine, pyridoxal and
pyridoxamine
All the three are equally active as vitamins
73. C H O H
|
2 C H O
|
C H N H2 2
|
— C H O H2 — C H O H2 — C H O H2H O — H O — H O —
H C —3 H C —3 H C —3
N N N
P y r id o x in e P y r id o x a l P y r id o x a m in e
74. Functions
Pyridoxine, pyridoxal and pyridoxamine are phos-
phorylated by ATP in the presence of pyridoxal
kinase to form pyridoxine phosphate, pyridoxal
phosphate and pyridoxamine phosphate
respectively
The three are interconvertible
75. Subjects deficient in thiamin retain most of the
test dose in tissues and excrete less in urine
Measurement of transketolase activity in
erythrocytes can confirm the diagnosis
C H O H
|
2
C H O
|
C H N H2 2
|
— C H O H2
— C H O H2
— C H O H2
H O —
H O —
H O —
H C —3
H C —3
H C —3
N
N
N
C H O H
|
2
C H O
|
C H N H
|
2 2
H O —
H O —
H O —
H C —3
H C —3
H C —3
N
N
N
— C H O — P — O H2 —
O
||
|
O H + A D P+ A T P
+ A T P
+ A T P
P y r id o x a l
k in a s e
P y r id o x a l
k in a s e
P y r id o x a l
k in a s e
P y r id o x in e
P y r id o x a l
P y r id o x a m in e
P y r id o x in e p h o s p h a te
P y r id o x a l p h o s p h a te ( P L P )
P y r id o x a m in e p h o s p h a te
— C H O — P — O H2 —
O
||
|
O H + A D P
— C H O — P — O H2 —
O
||
|
O H + A D P
76. Pyridoxal phosphate and pyridoxamine
phosphate serve as coenzymes, mainly in the
metabolism of amino acids
Pyridoxal phosphate (PLP) can form a Schiff
base with an α-amino acid
The union occurs between the α-amino group of
the amino acid and the aldehyde group of
pyridoxal phosphate
77. The amino acid, thus bound, can undergo
various reactions
R — C H — C O O H
|
N
| |
C — H
|
H O —
H C —3
N
— C H O — P2 —
Schiff base
79. Transamination
These reactions are catalysed by specific
transaminases
The amino group of an amino acid is transferred
to an α-keto acid forming a new amino acid and a
new α-keto acid
PLP acts as a carrier of the amino group
80. Transamination reactions are important in :
• formation of new amino acids
• catabolism of amino acids
81. Subjects deficient in thiamin retain most of the
test dose in tissues and excrete less in urine
Measurement of transketolase activity in
erythrocytes can confirm the diagnosis
N H 2 N H 2
| |
R — C H — C O O H1
R — C — C O O H1
R — C H — C O O H2
C H O
|
|
H O —
H O —
H C —3
H C —3
N
N
— C H O — P2 —
— C H O — P2 —
C H N H2 2
A m in o a c id
P y r id o x a l p h o s p h a te
P y r id o x a m in e p h o s p h a te
A m in o a c id
α - K e to a c id
O
||
R — C — C O O H2
α - K e to a c id
O
||
83. Decarboxylation
PLP is a coenzyme for decarboxylases acting on:
• glutamate
• arginine
• tyrosine
84. Transulphuration
PLP is a coenzyme for cystathionine synthetase
and cystathionine γ-lyase which transfer sulphur
from homocysteine to serine forming cysteine
85. C y s ta th io n in e
s y n th e t a s e
M e th io n in e a d e n o s y l
tr a n s fe r a s e
A T P P i+ P P i
C H — S — C H — C O O H3 2 C H — C H —2
|
N H 2
A d e n o s in e
M e t h y l t r a n s fe r a s e
R
R — C H 3
— S — C H — C O O H2 C H — C H —2
|
N H 2C H 3
H o m o c y s te in e
C y s t a t h io n in e H o m o s e r in e
M e t h io n in e S - A d e n o s y l m e th io n in e
S - A d e n o s y l h o m o c y s te in e
A d e n o s in e
A d e n o s y l
h o m o c y s te in a s e
A d e n o s in e
H O2
— S — C H — C O O H2 C H — C H —2
|
N H 2
C H — O H
|
C H — C O O H
|
N H
S e r in e
2
2
H O2
H S — C H — C O O H2 C H — C H —2
|
N H 2
C y s t a th io n in e
- ly a s e
C H — S H2
|
C H — C O O H
|
N H 2
C y s te in e
H O2
C H — S — C H — C O O H2 2 C H — C H —2
|
N H 2
|
C H — C O O H
|
N H 2
H O — C H — C O O H2 C H — C H —2
|
N H 2
~
γ-
PLP
PLP
86. Desulphydration
PLP is a coenzyme for cysteine desulphydrase
which removes the –SH group from cysteine
87. Tryptophan metabolism
One of the intermediates in the catabolism of
tryptophan is 3-hydroxykynurenine which is
converted into 3-hydroxyanthranilic acid by
kynureninase, a PLP-dependent enzyme
88. When PLP is not available, 3-hydroxyanthranilic
acid is not formed, and 3-hydroxykynurenine is
spontaneously converted into an alternate
metabolite, xanthurenic acid which is excreted in
urine
Urinary excretion of xanthurenic acid can serve
as an indicator of pyridoxine deficiency
89. Subjects deficient in thiamin retain most of the
test dose in tissues and excrete less in urine
Measurement of transketolase activity in
erythrocytes can confirm the diagnosis
T ry p to p h a n 3 -H y d ro x y k y n u re n in e
S p o n ta n e o u s
X a n th u re n ic a c id
P L P K y n u re n in a s e
3 -H y d ro x y a n th ra n ilic a c id E x c re te d in u rin e
A c e to a c e ty l C o A
90. Synthesis of haem
One of the enzymes involved in the synthesis of
haem is δ-aminolevulinic acid synthetase which
requires PLP as a coenzyme
91. Cellular uptake of amino acids
Cellular uptake of L-amino acids is an active
process which requires the participation of PLP
92. Formation of γ-amino butyric acid
Gamma-amino butyric acid (GABA) acts as a
neurotransmitter in brain
It is formed by the action of glutamate
decarboxylase on glutamate
PLP is required as a coenzyme in this reaction
94. Sources
Liver, eggs, milk, yeast, wheat, corn and green
leafy vegetables are excellent sources of the
vitamin
Another source is bacterial synthesis in the
intestine
95. Requirement
Since this vitamin is mainly required in the
metabolism of amino acids, its requirement depends
upon the protein intake
An intake of 1.25 mg /100 gm of proteins has
been recommended
96. Deficiency
Deficiency is very rare
It may sometimes occur in infants and pregnant
women
Deficiency may also occur in patients taking
isoniazid, an anti-tuberculosis drug, which forms a
complex with pyridoxal and prevents its activation
97. The clinical features of deficiency are nausea,
vomiting, dermatitis, microcytic anaemia and
convulsions
Convulsions are more common in children while
anaemia is more common in adults
Chronic deficiency may cause
hyperhomocysteinaemia which increases the risk of
cardiovascular diseases
98. Laboratory diagnosis of deficiency can be made
by measuring the urinary excretion of xanthurenic
acid after a test dose of tryptophan
The urinary excretion of xanthurenic acid is
increased in pyridoxine deficiency
99. BIOTIN
Biotin is also known as anti-egg white injury
factor
When raw egg white is fed to rats, they develop
certain symptoms which are relieved by biotin
It has been shown that raw egg white contains a
protein, avidin, which forms a complex with biotin
preventing its intestinal absorption
100. This leads to a deficiency of biotin
Avidin is inactivated by heat
Therefore, cooked eggs do not hamper
absorption of biotin
Biotin is heat-stable
101. Biotin is a heterocyclic, sulphur-containing,
monocarboxylic acid
H N
|
H C
|
H C2
N H
|
C H
|
C H — ( C H ) — C O O H2 4
O
||
C
S
102. Functions
Biotin is a coenzyme for carboxylases and is,
therefore, also known as co-carboxylase
The carboxyl group of biotin forms a bond with
the epsilon-amino group of a lysine residue of the
apoenzyme
Thus, biotin is firmly bound to the enzyme
103. Some examples of carboxylation reactions in
which biotin is required are:
• Carboxylation of pyruvate
• Carboxylation of acetyl CoA
• Carboxylation of propionyl CoA
104. Carboxylation of pyruvate
This reaction converts pyruvate into
oxaloacetate
As oxaloacetate is an intermediate in citric acid
cycle, this reaction is important for the normal
operation of citric acid cycle
105. C H 3
|
C = O
|
C O O H
+ C O + A T P2
P y r u v a t e
C O O H
|
C H 2
|
C = O
|
C O O H
+ A D P + P i
O x a lo a c e t a t e
P y r u v a t e
c a r b o x y l a s e
B i o t i n
106. Carboxylation of acetyl CoA
This reaction converts acetyl CoA into malonyl
CoA, and is important in fatty acid synthesis
C H — C ~ S — C o A3
C H — C ~ S — C o A2 + A D P + P i
+ C O + A T P2
A c e t y l C o A
M a lo n y l C o A
A c e t y l C o A
c a r b o x y la s e
B i o t i n
O
| |
O
| |
C O O H
107. Carboxylation of propionyl CoA
Propionyl CoA is carboxylated to D-
methylmalonyl CoA
This is one of the reactions in the gluconeogenic
pathway for conversion of propionate into
glucose
108. C H 3
C H 3
|
|
C H 2
H — C — C O O H
|
|
O = C ~ S — C o A
O = C ~ S — C o A
+ C O + A T P2
+ A D P + P i
P r o p io n y l C o A
D - M e th y lm a lo n y l C o A
P r o p io n y l C o A
c a r b o x y la s e
B io tin
109. Sources
Bacterial synthesis in the intestine provides
ample amounts of biotin
Dietary sources include egg yolk, liver, kidney,
yeast, peas, tomatoes, cauliflower etc
110. Requirement
Biotin requirement is not known with certainty as
the intestinal bacteria meet most of the
requirement
The daily intake has been estimated to be 100 to
300 µg
111. Deficiency
Deficiency of biotin is unknown in human beings
as sufficient vitamin is provided by intestinal
bacteria, and the vitamin is widely distributed in
both animal and plant foods
Deficiency may occur in animals when they are
fed raw egg white
It is clinically characterised by retarded growth,
loss of weight, dermatitis, loss of hair, muscular
inco-ordination and paralysis
112. LIPOIC ACID
Lipoic acid is a sulphur-containing fatty acid
It is also known as thioctic acid or 6,8-dithio-
octanoic acid
It can exist in a reduced form and an oxidised
form
113. C H — C H — C H — (C H ) — C O O H2 2 2 4 C H — C H — C H — (C H ) — C O O H2 2 2 4
α -L ip o ic a c id ( re d u c e d ) α -L ip o ic a c id ( o x id is e d )
| || |
S H SS H SA A H 2
114. Functions
Lipoic acid acts as a coenzyme in some
oxidation-reduction reactions because of its ability to
undergo reversible oxidation and reduction
It is required as a coenzyme in the oxidative
decarboxylation of α-keto acids such as pyruvate
and α-ketoglutarate
115. Sources
Lipoic acid is widely distributed in foodstuffs
The quantities required by human beings are
easily obtained from an ordinary diet
116. Requirement
The exact requirement for lipoic acid is not
known
The vitamin is probably required in very minute
quantities
117. Deficiency
Deficiency of lipoic acid has not been reported in
human beings
Attempts to induce lipoic acid deficiency in higher
animals have also been unsuccessful
118. FOLIC ACID
Folic acid is also known as folacin or
pteroylglutamic acid
It is made up of pteridine, para-aminobenzoic
acid and glutamic acid
119. H N2 N
N
|
O H
1
2
3
4
N
5
6
7
8
N
9 1 0
C H — N —2 — C — N — C H
| |
H C O O H
C O O H
|
C H 2
|
C H 2
|
O
||
H
|
P te r id in e p a r a -A m in o -
b e n z o ic a c id
G lu ta m ic
a c id
P te r o y lg u ta m ic a c id (fo lic a c id )
120. Folic acid is found in foodstuffs as pteroylmono-
glutamate, pteroyltriglutamate and
pteroylheptaglutamate
The last two are converted into
pteroylmonoglutamate in the intestinal mucosa (by
folate conjugase)
Folic acid is heat-stable in neutral medium
121. Functions
Folic acid forms a coenzyme, tetrahydrofolate
Folic acid is first reduced to 7,8-dihydrofolate (H2-
folate or FH2) and then to 5,6,7,8-tetrahydrofolate
(H4-folate or FH4) by dihydrofolate reductase
122. Amethopterin (methotrexate) and aminopterin are
competitive inhibitors of dihydrofolate
reductase, and act as folic acid antagonists
D ih y d r o f o la t e
r e d u c ta s e
D ih y d r o f o la t e
r e d u c ta s e
F o la t e
H - f o la t e2
H - f o la t e4
N A D P H + H
+
N A D P H + H
+
N A D P
+
N A D P +
123. H4-Folate is a carrier of one-carbon units e.g.
• Formyl (–CHO) group
• Formate (–HCOO-
) group
• Methyl (– CH3) group
• Methylene (=CH2) group
• Methenyl (=CH) group
• Formimino (–CH = NH) group
124. The one-carbon unit may be attached to N5
or N10
of H4-folate
125. H N2 N
N
N
H
5
H
N
1 0
C H — N —2 — C — N — C H
| |
C H O C O O H
C O O H
|
C H 2
|
C H 2
|
O
| |
H
|
H N2 N
N
N
|
C H 3
5
H
N
1 0
C H — N —2 — C — N — C H
| |
H C O O H
C O O H
|
C H 2
|
C H 2
|
O
| |
H
|
N - M e t h y l- H - f o l a t e5
4
N - F o r m y l - H - f o l a t e ( f - H - f o l a t e )1 0 1 0
4 4
H N2 N
N
N
|
C H
| |
N H
5
H
N
1 0
C H — N —2 — C — N — C H
| |
H C O O H
C O O H
|
C H 2
|
C H 2
|
O
| |
H
|
N - F o r m i m i n o - H - f o l a t e ( f i - H - f o l a t e )
5 5
4 4
|
O H
|
O H
|
O H
126. H4-Folate can:
• receive one-carbon units from various
compounds
• transfer these for the synthesis of various
compounds
127. Sources of one-carbon units
Tetrahydrofolate may receive one-carbon units
from:
• Formiminoglutamic acid
• Methionine
• Choline
• Thymine
• Serine
128. Formiminoglutamic acid (FIGLU) is formed in the
body from histidine
FIGLU can transfer its formimino group to
tetrahydrofolate
FIGLU + H-Folate4 fi-H-Folate + Glutamate
5
4
129. Methionine, choline and thymine are the source
of methyl group
Serine can contribute its hydroxymethyl group
130. Utilisation of one-carbon units
The one-carbon unit carried by tetrahydrofolate
can be utilised in the following reactions:
• Synthesis of purines
• Synthesis of serine
• Synthesis of methionine
• Synthesis of choline
• Synthesis of thymine
• Synthesis of n-formylmethionine
131. Synthesis of purines
The carbon atoms 2 and 8 of purines are
contributed by f10
-H4-folate
132. Synthesis of serine
The hydroxymethyl group for the conversion of
glycine into serine is provided by N5
, N10
-
methylene-H4-folate
133. Synthesis of methionine
The methyl group for the conversion of
homocysteine into methionine is provided by N5
-
methyl-H4-folate
134. Synthesis of choline
The methyl groups for the synthesis of choline
from serine are provided by N10
-methyl-H4-folate
135. Synthesis of thymine
The methyl group of thymine is provided by N5
,
N10
-methylene-H4-folate
136. Synthesis of n-formylmethionine
The formyl unit of f10
-H4-folate converts
methionine into n-formylmethionine which initiates
protein synthesis in prokaryotes
137. Sources
Folic acid is obtained from green leafy
vegetables, yeast, liver, kidney, meat, fish, milk etc
Intestinal bacteria also synthesise folic acid
138. Requirement
Infants and children : 100 µg/day
Adult men and women : 100 µg/day
Pregnant women : 300 µg/day
Lactating women : 150 µg/day
139. Deficiency
As folic acid is required for the synthesis of
purines and thymine, its deficiency impairs the
synthesis of nucleic acids
This leads to growth failure and megaloblastic
anaemia
140. Leukopenia can also occur
Laboratory diagnosis of deficiency can be made
by giving a test dose of histidine and measuring
the urinary excretion of FIGLU which is increased
in subjects deficient in folic acid
141. COBALAMIN (Vitamin B12)
Cyanocobalamin was the first B12 vitamin isolated
as a red crystalline compound from liver in 1948
Vitamin B12 activity was later found in several
compounds in which the cyanide group is replaced
by a hydroxyl group (hydroxycobalamin), a methyl
group (methylco- balamin), a nitro group
(nitrocobalamin) or a chloride group
(chlorocobalamin)
142. Vitamin B12 has a complex structure
Molecular formula of cyanocobalamin is
C63H90O14N14PCo
It has four pyrrole rings with a cobalt atom at the
centre (corrin ring)
The tetrapyrrole is heavily reduced and
substituted
143. The cobalt atom forms co-ordination bonds with
the nitrogen atoms of four pyrrole rings, a
cyanide group and 5,6-dimethylbenzimidazole
which, in turn, is linked with ribose-3-phosphate
The phosphate group of ribose-3-phosphate is
linked with the pyrrole ring D (IV) through
amino propanol
144.
145. Vitamin B12 is heat-stable in acidic and neutral
medium
It is present in food in association with proteins
146. Absorption, Transport and Storage
The ingested vitamin B12 is released by gastric
hydrochloric acid
Most of the vitamin binds to R-protein
(cobalophilin) secreted in gastric juice and saliva
Gastric parietal cells also secrete intrinsic factor
(IF), a glycoprotein of 45 kD which can bind
vitamin B12
147. However, at low pH, the affinity of vitamin B12 for
R-protein is much higher than that for IF
Therefore, most of the vitamin binds to R-protein
in stomach
In the duodenum, R-protein is hydrolysed, and
vitamin B12 is bound to IF
148. One IF molecule binds one molecule of vitamin
B12
A specific receptor on ileal mucosa binds the
IF:vitamin B12 complex
The vitamin is taken up by the mucosal cells and
is transferred to plasma
149. Most of the vitamin is bound to transcobalamin II
in plasma
The circulating transcobalamin II:vitamin B12
complex is taken up by cells with the help of a
specific receptor
Transcobalamin II is hydrolysed in the cell by
lysosomal enzymes
150. A significant amount of vitamin B12 (deoxyadenosy
cobalamine) is stored in the body, principally in
liver
Most of the vitamin is bound to transcobalamin I
in liver
151. Absorption and storage of
vitamin B12
R = cobalophilin;
Cbl = cobalamin;
IF = intrinsic
factor;
TC = trans cobalamin
152. Functions
Vitamin B12 forms coenzymes known as
cobamides which take part in a number of
metabolic reactions
Cobamides are formed by replacement of the
cyanide group of vitamin B12 by 5´-deoxyadenosine
153. There are three cobamides:
• 5,6 - Dimethylbenzimidazole cobamide:
5,6-Dimethylbenzimidazole is present as such
as in vitamin B12
• Benzimidazole cobamide: Benzimidazole is
present instead of 5,6-dimethylbenzimidazole
• Adenyl cobamide: Adenine replaces 5,6-
dimethylbenzimidazole
154. Besides these three, methylcobalamin is active
as a coenzyme as such
The cobamides function as coenzymes in the
various reactions
155. Transfer of one-carbon units
Apart from tetrahydrofolate, cobamides are also
involved in the transfer of one-carbon units
In fact, they act in concert
An example of one such reaction is the synthesis
of methionine from homocysteine
156. H4-Folate is returned to the folate pool so that it
can again participate in various one-carbon
transfer reactions
N -M e th y l- H -fo la te5
4 H -F o la te4
C o b a la m in M e th y lc o b a la m in
M e th io n in e H o m o c y s te in e
157. In cobalamin deficiency, H4-folate can not return
to folate pool and is trapped
This is known as folate trap
Thus, cobamides help in one-carbon transfer
reactions by sharing a part of the load on H4- folate
158. Formation of succinyl CoA
Cobamides act as a coenzyme in the conversion
of methylmalonyl CoA into succinyl CoA
Methylmalonyl CoA is formed directly from valine
and via propionyl CoA from isoleucine,
methionine and fatty acids having an odd number of
carbon atoms
Succinyl CoA may be converted into glucose or
oxidised in citric acid cycle
159. C H 3
|
H O O C — C — H
|
C ~ S — C o A
M e th y lm a lo n y l C o A is o m e r a s eC o b a m id e
L - M e th y lm a lo n y l C o A
S u c c in y l C o A
||
O
|
C H — C ~ S — C o A2
||
O
C H — C O O H2
160. In vitamin B12 deficiency, methylmalonic acid is
excreted in large amounts in urine
(methylmalonic aciduria)
Rarely, methylmalonic aciduria may be
caused by an inherited defect in methylmalonyl
CoA isomerase
161. Sources
Vitamin B12 can not be synthesised by any plant
or animal
It is synthesised only by some bacteria
Animals acquire it through bacterial synthesis in
their intestines
162. Liver, kidney, meat, eggs, milk and cheese are
good sources of vitamin B12
Bacteria present in the human intestine also
synthesise vitamin B12
164. Deficiency
Deficiency of vitamin B12 can occur due to
deficient intake (nutritional deficiency) or due to
impaired absorption
Absorption is impaired when IF is absent due to
atrophy of gastric mucosa or after gastrectomy
The condition arising from absence of IF is
known as pernicious anaemia
165. Clinical features of deficiency may take long to
develop as the hepatic stores of vitamin B12 can
last a considerable length of time
Nutritional deficiency causes megaloblastic
anaemia
166. An additional feature in pernicious anaemia
due to absence of IF is sub-acute
combined degeneration
This is degeneration of lateral and posterior
columns of spinal cord leading to sensory as
well as motor disturbances
167. ASCORBIC ACID
Ascorbic acid (vitamin C) prevents a specific
deficiency disease, scurvy
Therefore, it is also known as anti-scorbutic
factor
It is very heat-labile, specially in basic medium
Chemically, its structure resembles that of
hexoses
168. It can exist as L- and D- isomers
Only the L-isomer possesses vitamin activity
It can be readily oxidised to dehydroascorbic
acid
Both L-ascorbic acid and L-dehydroascorbic acid
possess equal vitamin activity
169. C = O
|
C – O H
||
C – O H
|
H – C
|
H O – C – H
|
C H O H2
H – C
C = O
|
|
|
|
H O – C – H
|
C H O H2
C = O
C = O
A A H 2
L - A s c o r b ic a c id L - D e h y d r o a s c o r b ic a c id
O O
170. Vitamin C is synthesised by all plants and
animals except guinea pigs and primates which
lack L-gulonolactone oxidase, the enzyme that
converts L-gulonolactone into L-ascorbic acid
171. Functions
Vitamin C is required for the formation and
maintenance of intercellular cement substance
Since it can undergo reversible oxidation and
reduction, it takes part in some oxidation-
reduction reactions
There is considerable evidence in support of the
role of vitamin C in the oxidation of phenylalanine
and tyrosine
172. It is required for post-translational hydroxylation
of proline and lysine residues, and is, therefore,
essential for the conversion of procollagen into
collagen which is rich in hydroxyproline and
hydroxylysine
Through collagen synthesis, it plays a role in the
formation of matrix of bones, cartilages, dentine
and connective tissue
173. By converting ferric ions into ferrous ions, it helps
in the absorption of iron
It is required for the formation of bile acids from
cholesterol
It acts as an anti-oxidant
It inhibits the formation of nitrosamines
(carcinogens) during digestion
174. Ascorbic acid may play a role in the
synthesis of adrenocortical steroid hormones as
ascorbic acid content of adrenal gland is found
to decrease rapidly in stressful conditions
175. Tissue Distribution
Total amount of ascorbic acid in an adult is 2 - 3
gm
It is distributed in all tissues and body fluids
It is present in high concentrations in the glands
176. The highest concentration is found in the
adrenal glands, followed by the pituitary gland,
thymus and corpus luteum
Leukocytes are also rich in ascorbic acid
The concentration in plasma is 0.5 -1.5 mg/dl
The vitamin begins to appear in urine when the
plasma level exceeds 1 mg/dl
177. Sources
Citrus fruits, e.g. amla, lemon and orange, are
very rich in vitamin C
Guava, tomatoes, green leafy vegetables
and germinating pulses are also good sources
178. Potatoes are a fair source
Since considerable losses of vitamin C can
occur during cooking, some raw fruits and salads
should be included in the daily diet
179. Requirement
Vitamin C is required in much larger quantities
than any other vitamin
The RDA according to Indian Council of
Medical Research is :
Infants and children : 30-50 mg/day
Adult men and women : 60 mg/day
Pregnant and lactating women : 80 mg/days
180. Deficiency
Deficiency of vitamin C produces scurvy
A full-blown picture of scurvy is rare these days
but isolated signs and symptoms of vitamin C
deficiency are still seen
181. The signs and symptoms include:
• Swollen, spongy and bleeding gums
• Loosening of teeth
• Petechial haemorrhages
• Anaemia
• Retardation of skeletal growth
• Easy fracturability of bones
• Delayed union of fractures
• Delayed healing of wounds
• Hypercholesterolaemia
182. Laboratory diagnosis of deficiency can be made
by ascorbic acid saturation test
After a test dose of ascorbic acid, urinary
excretion of ascorbic acid is low in subjects deficient
in the vitamin
183. VITAMIN A
Vitamin A is found only in animals though its
precursors are found in a variety of plants
It occurs in three forms, retinol (alcohol form),
retinal (aldehyde form) and retinoic acid (acid form)
Chemically, each form contains a β-ionone ring
attached to a polyene chain
184. HH H H
C H 3
C H 3
C H 3
|
|
|
H
H
H
|
|
|
C
C
C
1
2
3
4
5
6
7
8
C H 3
C H 3
C H 3
C H 3
C H 3
C H 3
C
C
C
C H 3
C H 3
C H 3
|
|
|
C
C
C
9 1 0
C
C
C
H
H
H
|
|
|
C
C
C
1 1
1 2
C
C
C
|
|
|
C
C
C
1 3 1 4
C
C
C
C H O H2
C H O
C O O H
1 5
C H 3
C H 3
C H 3
R e tin o l
R e tin a l
R e tin o ic a c id
HH H H
HH H H
185. All the double bonds in the polyene chain have a
trans-configuration in naturally occurring vitamin
A (all-trans-vitamin A)
186. Retinol may be esterified with a fatty acid or
phosphoric acid
The storage form of vitamin A is generally retinyl
palmitate
Some of the functions of vitamin A are performed
by retinyl phosphate
Vitamin A can be formed in our body from its
187. The precursors are carotenes (or carotenoids)
which are naturally occurring pigments found in
most yellow and green fruits and vegetables
α-Carotene, β-carotene and γ-carotene are
important precursors of vitamin A
Of these, β-carotene is the most important
One molecule of β-carotene can be converted
into two molecules of retinal
188. A molecule of β−carotene contains two β−ionone
rings connected by an 18-carbon polyene
chain
β-Carotene is cleaved in the middle by β-
carotene dioxygenase and molecular oxygen to
form two molecules of retinal
Bile salts facilitate the reaction
189. α-Carotene and γ-carotene contain only one β-
ionone ring, and can form only one retinal
molecule each
Retinal is reduced to retinol by retinaldehyde
(retinine) reductase
Some retinal is spontaneously oxidised to retinoic
acid
190. While retinal and retinol are interconvertible,
retinoic acid cannot be converted back into retinal
191. β - C a r o t e n e
[ O ]2
β - C a r o t e n e d io x y g e n a s e ,
b ile s a lt s
R e t in o l
N A D P H + H +
N A D P +
R e t in a ld e h y d e r e d u c t a s e
( r e t in e n e r e d u c t a s e )
C H 3
|
C
C H 3
C H 3
C
H
|
H C3
C
C
H
|
C
C
H
|
C
C
H
|
C H 3 C H 3
| | | |
H H H H H
| | | | |
H H H C3
C
C
H
|
C
C
|
C
C
H
|
C
C
|
C
C
H
|
C H 3C H 3
C H 3
|
C
C H 3
C H 3
C
H
|
H C3
C
C
H
|
C
C
H
|
C
C
H
|
C H 3 C H 3
| | | |
H H H H
| | | |
H H H C3
C H O
O H C
C
C
|
C
C
H
|
C
C
|
C
C
H
|
C H 3C H 3
+
C H 3
|
C
C H 3
C H 3
C
H
|
C
C
H
|
C
C
H
|
C
C
H
|
C H 3
C H 3
| |
H H
| |
C H O H2
R e t in o ic a c id
S p o n ta n e o u s
[ O ]
C H 3
C H 3
| | |
H H
| |
C
C
H
|
C C
C C
| |
C
C
H
|
C H 3
C H 3 C H 3
C O O H
H H
|
C H 3
|
C H 3
R e t in a l R e t in a l
192. Vitamin A is absorbed from the small intestine
Retinyl esters are hydrolysed to form retinol
Retinol is taken up by the mucosal cells, and is
re-esterified with saturated fatty acids
Esterified retinol enters the lacteals and reaches
liver via circulation
193. It is stored in the hepatic lipocytes
Conversion of carotenes into retinal and, then,
retinol occurs in the small intestine
A small amount of carotenes may be absorbed
as such and transported by chylomicrons
194. Retinol is released from hepatic cells into
circulation
It is transported in blood by an α1-globulin, retinol
binding protein (RBP)
Circulating retinol is taken up by various cells in
which it is bound to cellular retinol binding protein
(CRBP)
Retinoic acid is transported in circulation in
association with albumin
195. Functions
• Reproduction
• Growth and differentiation
• Integrity of epithelial tissues
• Vision
• Anti-oxidant role
• Anti-carcinogenic role
196. Reproduction
Vitamin A deficiency has been observed to impair
reproductive function in some animals
This function of vitamin A is probably mediated
by control of expression of certain genes by
retinol bound to CRBP
197. Growth and differentiation
Vitamin A is required for normal growth and
differentiation of tissues
Both skeletal growth and formation of soft tissues
are impaired in growing animals deficient in
vitamin A
Vitamin A seems to be essential for the synthesis
of mucopolysaccharides and glycoproteins in
various tissues
198. Integrity of epithelial tissues
Vitamin A maintains the integrity of epithelial
tissues
The epithelial tissues of eyes, lungs,
gastrointestinal tract and genitourinary tract become
dry and keratinised in vitamin A deficiency
199. Vision
Role of vitamin A in vision was demonstrated by
Morton and Wald
Retina contains two types of photoreceptor cells,
rods and cones
Rod cells are required for dim light vision and
cone cells for bright light vision including the
perception of colours
200. Vitamin A is essential for the functioning of rod
cells as well as cone cells
Rod cells contain a pigment, rhodopsin (visual
purple) which is a conjugated protein made up of
opsin (a protein) and 11-cis-retinal, an isomer of
all-trans-retinal
201. The aldehyde group of 11-cis-retinal is bonded
with the epsilon-amino group of a lysine residue
of opsin by Schiff base linkage
Rhodopsin molecules are present in large
numbers in rod cells
When light strikes rod cells, 11-cis-retinal is
converted into all-trans-retinal
202. Conformation of all-trans-retinal is such that it
can not bind with opsin
Therefore, opsin and all-trans-retinal dissociate
A number of transient intermediates are formed
before dissociation of opsin and all-trans-retinal
203. When a person moves from dim light into bright
light, all the rhodopsin molecules are rapidly
dissociated
When he moves back into dim light, he is unable
to see until rhodopsin is re-formed
For this, all-trans-retinal has to be isomerised to
11-cis-retinal
204. The enzyme system for the isomerisation
reaction is present only in liver
Therefore, all-trans-retinal is reduced to all-trans-
retinol in retina, and is released into circulation
It is taken up by liver, and is converted into 11-
cis-retinol which is released into circulation
205. Retina takes up the circulating 11-cis-retinol and
oxidises it to 11-cis-retinal
The latter combines with opsin to form rhodospin
This sequence of reactions is known as visual
cycle
206. R h o d o p s in
L ig h t L ig h t
B a th o r h o d o p s in L u m ir h o d o p s in
M e ta r h o d o p s in I
M e ta r h o d o p s in II
O p s in
A ll- - r e tin a ltr a n s
A ll- - r e tin o ltr a n s
A ll- - r e tin o ltr a n s
II- - R e tin a lc is
II- - R e tin o lc is
II- - R e tin o lc is
N A D P H + H
+
R e tin in e r e d u c ta s e
N A D P
+
v ia
c ir c u la tio n
v ia
c ir c u la tio n
R e tin o l is o m e r a s e
R E T IN A
L IV E R
L ig h t
L ig h t
L ig h t
207. The time taken for regeneration of rhodospin is
known as the dark adaptation time which
depends upon the vitamin A content of liver
When liver contains adequate stores of vitamin
A, the dark adaptation time is short
In vitamin A deficiency, dark adaptation time is
prolonged
208. Mechanism of vision
Rhodopsin is a transmembrane protein
When photons strike it, they cause a
conformational change in the rhodopsin molecule as
a result of which it is activated (photo-excited)
The photo-excited form is probably
metarhodopsin II
209. Another membrane-bound protein, transducin is
present in close vicinity of rhodopsin
Transducin belongs to the family of G-proteins,
and is made up of three subunits, α-subunit, β-
subunit and γ-subunit
The α-subunit has a site for GDP or GTP
Normally, this site is occupied by GDP
210. Photo-excited rhodopsin causes displacement of
GDP by GTP
The α-subunit containing GTP dissociates from
the β- and γ-subunits, and activates the enzyme,
cGMP phosphodiesterase
The active enzyme converts cGMP into G-5’-MP
Decrease in concentration of cGMP causes
hyperpolarisation of the rod cell membrane
211. The rod cell membrane contains a number of
cation-specific channels which are normally kept
open by cGMP leading to continuous influx of sodium
ions into the rod cell
The decrease in cGMP concentration leads to
closure of cation-specific channels, accumulation
of sodium ions outside the rod cell and the
consequent hyperpolarisation of the membrane
212. P h o t o - e x c it e d
r h o d o p s in
G D P - T r a n s d u c in G T P - T r a n s d u c in
G T P - - s u b u n itα β - a n d - s u b u n itγ
I n a c t iv e c G M P
p h o s p h o d ie s t e r a s e
A c t iv e c G M P
p h o s p h o d ie s t e r a s e
c G M P G – 5 ’– M P
O p e n
c a t io n - s p e c if ic
c h a n n e ls
C lo s e d
c a t io n - s p e c if ic
c h a n n le s
N o r m a l
r o d c e ll
m e m b r a n e
H y p e r p o la r is e d
r o d c e ll
m e m b r a n e
R h o d o p s in
L ig h t
213. The nerve impulse generated as a result of
hyperpolarisation is transmitted to appropriate
areas of brain (visual cortex)
The α-subunit of transducin possesses intrinsic
GTPase activity
Therefore, GTP bound to the α-subunit is slowly
hydrolysed into GDP
214. When GTP is converted into GDP, the α-subunit
re-associates with the β- and γ-subunits forming
transducin containing GDP
cGMP phosphodiesterase becomes inactive
again, and the whole chain of events is terminated
215. Vitamin A is also required for bright light vision
The cone cells of retina contain three conjugated
proteins, porphyrinopsin, iodopsin and
cyanopsin
These are sensitive to red, green and blue light
respectively
The prosthetic group is 11-cis-retinal in each of
these but the protein part is different
216. These proteins become photo-excited on
exposure to light of specific colour
A nerve impulse is, then, generated in much the
same way as in rod cells
A given cone cell contains only one of these
three proteins, and can perceive only one
particular colour
An inherited absence of, or defects in, these
proteins causes colour blindness
218. Anti-carcinogenic role
Some epidemiological studies have shown that a
low intake of vitamin A or carotenes is associated
with a high incidence of certain cancers
However, experimental and clinical confirmation
of this finding is yet to be obtained
219. Since retinol and retinal are interconvertable and
can also be converted into retinoic acid, they can
perform all the functions of vitamin A
Retinoic acid can support growth and
differentiation, and can maintain epithelia but cannot
perform other functions of vitamin A
13-cis-Retinoic acid, a synthetic compound, is
more active than naturally occurring retinoic acid
220. Sources
Preformed vitamin A is present only in animal
foods e.g. fish liver oils, liver, eggs, milk, butter,
cream, cheese etc
Provitamins A are found in green leafy
vegetables, e.g. spinach, coriander leaves, mustard
leaves, cabbage, lettuce etc, and in red and yellow
vegetables and fruits e.g. carrot, tomato, mango,
papaya, plums etc
221. Requirement
Since vitamin A is present in food in many
different forms which differ in their vitamin A activity,
it is not possible to express the requirement for
vitamin A in terms of its actual weight
For instance, if we take equal amounts of retinol,
β-carotene and α- or γ-carotene, their vitamin A
activities would be different
222. To overcome this difficulty, relative vitamin A
activities of different compounds were compared in
rats, and were expressed in terms of international
units (IU)
One IU is the activity present in 0.3 µg of retinol,
0.344 µg of retinyl acetate or 0.6 µg of β-
carotene
Daily requirements were expressed in IU for a
long period
223. Subsequently, it was found that intestinal
absorption and utilisation of carotenes were much
less efficient in human beings than in rats
Therefore, vitamin A activities were converted
into retinol equivalents (RE)
One RE is the activity present in 1 µg of retinol, 6
µg of β-carotene or 12 µg of other carotenes
224. The daily requirement of vitamin A in terms of IU
and RE is :
IU/day RE/day
Infants 1,500-2,000 400
Children 2,000-4,000 400-700
Adult men 5,000 1,000
Adult women 4,000 800
Pregnant women 5,000 1,000
Lactating women 6,000 1,200
225. Deficiency
A deficiency of vitamin A can arise from
inadequate intake, inadequate absorption or
inadequate conversion of carotenes into vitamin A
Zinc deficiency may impair the mobilisation of
vitamin A from liver
226. The clinical manifestations of deficiency are:
• Xerophthalmia
• Keratomalacia
• Nyctalopia
• Blindness
• Follicular hyperkeratosis
• Susceptibility to infections
227. Xerophthalmia
The lacrimal glands become keratinised, and
stop secreting lacrimal fluid
The eyes become dry
Bitot’s spots (small, opaque spots) may appear
on cornea
229. Nyctalopia
Initially, dark adaptation time is prolonged which
may be the earliest indication of deficiency
Finally, the ability to see in dim light is completely
lost which is known as night blindness or
nyctalopia
230. Blindness
In severe and prolonged deficiency, there is total
loss of vision due to functional and structural
changes in the eyes
233. Vitamin A Toxicity
Hypervitaminosis A may occur if large doses of
vitamin A, usually in pharmacological form, are
taken over long periods
It is characterised by rough skin, hair loss,
anorexia, weight loss, headache, vertigo,
irritability, hyperaesthesia, hepatosplenomegaly,
liver damage etc
234. VITAMIN D
Vitamin D is also known as anti-rachitic factor or
anti-rachitic vitamin as it prevents a deficiency
disease, rickets (rachitis)
Of the several compounds possessing vitamin D
activity, the most important are vitamin D2
(ergocalciferol) and vitamin D3 (cholecalciferol)
235. Vitamin D2 is formed from provitamin D2
(ergosterol) on exposure to ultraviolet light
Vitamin D3 is formed from provitamin D3
(dehydrocholesterol) on exposure to ultraviolet
light
236. H C3
H C3
H C3
H C3
C H 3 C H 3
H O
H O
H O
H O
C H 3
C H 3
C H 3
C H 3
C H 3
C H 3
C H 2
C H 2
C H 3
C H 3
C H 3
C H 3
C H 3
C H 3
C H 3
C H 3
U lt r a v io le t lig h t
U lt r a v io le t lig h t
E r g o s t e r o l
7 - D e h y d r o c h o le s t e r o l
E r g o c a lc if e r o l ( v ita m in D )2
C h o le c a lc if e r o l ( v ita m in D )3
237. Ergosterol occurs in plants e.g. ergot and yeast
7-Dehydrocholesterol occurs in animals
It is formed from cholesterol
7-Dehydrocholesterol present in skin is converted
into cholecalciferol on exposure to sunlight
Cholecalciferol is synthesised in human beings
also
238. Functions
• Increased intestinal absorption of calcium and
phosphorus
• Increased renal tubular absorption of calcium
• Mineralisation of bones
239. Vitamin D increases the intestinal absorption of
calcium
The absorption of phosphorus is also increased
secondarily
Vitamin D increases the renal tubular absorption
of calcium, and decreases that of phosphorus
240. Vitamin D is required for mineralisation of bones
Bone formation is a continuous and dynamic
process
Calcium salts are continuously deposited into
and resorbed from bones by osteoblasts and
osteoclasts respectively
241. In growing age, deposition exceeds resorption
leading to skeletal growth
In adult life, deposition and resorption are finely
balanced leading to remodeling of bones
Vitamin D is required for bone growth as well as
remodeling
242. Thus, the main functions of vitamin D are to
regulate the metabolism of calcium and the
mineralisation of bones
However, these functions are not performed by
vitamin D itself
Vitamin D is first hydroxylated at C25 to form
25-hydroxycholecalciferol in the liver
243. 25-Hydroxycholecalciferol is further hydroxylated
at C1 in the kidneys to form 1,25-
dihydroxycholecalciferol (1,25-DHCC or calcitriol)
1,25-DHCC is the metabolically active form of
vitamin D
1,25-DHCC acts as a hormone and,
therefore, vitamin D may be regarded as a
prohormone
244. The mechanism of action of 1,25-DHCC is similar
to that of steroid and thyroid hormones
It binds to its intracellular receptors in the target
cells
The hormone-receptor complex binds to a
hormone response element in DNA and increases the
transcription and translation of certain genes
245. C h o le c a lc ife r o l
H y d r o x y la s e
2 5 - H y d r o x y c h o le c a lc ife r o l
P a r a th o r m o n e P a r a th y r o id
g la n d s
1 , 2 5 - D ih y d r o x y c h o le c a lc ife r o l
In d u c tio n
C a lc iu m - b in d in g p r o te in
C a - d e p e n d e n t A T P a s e
A lk a lin e p h o s p h a ta s e
+ +
C a lc iu m a b s o r p tio n R e le a s e in to c ir c u la tio n
P la s m a c a lc iu m
L IV E R
K ID N E Y
IN T E S T IN A L
M U C O S A
H y d r o x y la s e
IN T E S T IN A L
M U C O S A
⊕
–
↑ ↑
246. Sources
Plant foods are poor sources of vitamin D
Though ergocalciferol is present in some plants,
its intestinal absorption is poor
Cholecalciferol is the major dietary form of
vitamin D
Fish liver oils are very rich in cholecalciferol
247. Eggs, butter and cheese are fairly good sources
An important source of vitamin D is its
endogenous synthesis in the skin
Where sunshine is good and people are
adequately exposed to sunlight, enough vitamin D
is synthesised in the body to meet the daily
requirement
248. Requirement
Due to differences in the anti-rachitic activity of
different compounds possessing vitamin D
activity, the requirement is expressed in international
units (IU)
One IU is the activity present in 0.025 µg of
cholecalciferol
249. The requirement is 400 IU/day in infants,
children, and pregnant and lactating women
The adult requirement is 200 IU/day
250. Deficiency
Deficiency can occur due to inadequate intake,
inadequate absorption or inadequate exposure
to sunlight
Two distinct syndromes arise from deficiency of
vitamin D, rickets in childhood and osteomalacia
in adult life
251. RICKETS
The most susceptible periods for development of
rickets are infancy and puberty when skeletal
growth occurs rapidly
Deficiency of vitamin D causes deficient
mineralisation of bones and overgrowth of
epiphyses
This results in some typical skeletal deformities
252. Delayed closure of fontanelles
Closure of fontanelles is delayed in rickets
It causes bossing of skull
253. Craniotabes
The skull bones are soft due to poor
mineralisation
This is known as craniotabes
254. Pigeon chest
The antero-posterior diameter of chest is
increased
This gives the appearance of pigeon chest
255. Rickety rosary
The costochondral junctions of ribs are
enlarged, and appear to the beaded
This series of beads on the chest gives the
appearance of a rosary worn around the neck
256. Vertebral deformities
Kyphosis, lordosis or scoliosis can occur
Kyphosis is forward bending of vertebral column
Lordosis is backward bending of vertebral
column
Scoliosis is lateral bending of vertebral column
258. Bowlegs and knock knees
The leg bones may become curved under the
weight of the body (bowlegs)
The two knees may touch each other while
standing erect (knock knees)
259. Enlarged wrists and knees
Wrists and knees may be enlarged due to
overgrowth of epiphyses of the long bones
260. Apart from the skeletal deformities, some changes
in serum and urine chemistry are also seen
Serum calcium and inorganic phosphorus are
decreased
Serum alkaline phosphatase is increased
261. Hypocalcaemia increases the secretion of
parathormone
Urinary excretion of calcium is decreased, and that
of phosphorus is increased by parathormone
262. OSTEOMALACIA
Pregnant and lactating women are particularly
prone to osteomalacia
Demineralisation occurs to varying extents
Aches and pains may occur in bones and
susceptibility to fractures is increased
Changes in serum and urine chemistry are
similar to those in rickets
263. Toxicity
Prolonged intake of large doses of vitamin D in
pharmacological form may cause
hypervitaminosis D
Serum calcium is raised
Hypercalcaemia may cause loss of appetite,
polydipsia, polyuria, constipation, muscular
weakness etc
264. Calcification may occur in soft tissues e.g.
arteries, bronchi, muscles, kidneys etc
Stones may be formed in the kidneys
265. VITAMIN E
Vitamin E is sometimes described as anti-sterility
vitamin
However, its anti-sterility function is seen only in
some animals and not in human beings
Vitamin E activity is present in several
tocopherols, the most important being α-, β-, γ-
and δ- tocopherols
266. ( C H ) — C H — ( C H ) — C H — ( C H )2 3 2 3 2 3 — C H — ( C H )3 2
( C H ) — C H — ( C H ) — C H — ( C H )2 3 2 3 2 3 — C H — ( C H )3 2
( C H ) — C H — ( C H ) — C H — ( C H )2 3 2 3 2 3 — C H — ( C H )3 2
( C H ) — C H — ( C H ) — C H — ( C H )2 3 2 3 2 3 — C H — ( C H )3 2
H O —
H O —
H O —
H O —
H C —3
H C —3
C H 3
C H 3
C H 3
C H 3
C H 3
C H 3
C H 3
C H 3
C H 3
C H 3
|
|
|
|
|
|
1
2
3
45
6
7
8
O
O
O
O
C H 3
C H 3
C H 3
C H 3
C H 3
C H 3
C H 3
C H 3
|
|
|
|
|
|
|
|
α - T o c o p h e r o l
γ- T o c o p h e r o l
δ - T o c o p h e r o l
β - T o c o p h e r o l
267. α-Tocopherol is the most abundant tocopherol in
foods, and is taken as the standard
The official reference standard is synthetic DL-α-
tocopherol acetate
The vitamin E activity present in 1 mg of this
compound is described as one international unit
(IU)
268. The vitamin E activity of 1 mg of naturally-
occurring D-α-tocopherol is 1.5 IU
The vitamin E activities of other tocopherols are
much lower
269. Functions
The most important function of vitamin E in
human beings is to act as an anti-oxidant
Vitamin E is readily oxidisable, and prevents the
oxidation of other, less oxidisable compounds
It prevents the oxidation of other antioxidants e.g.
carotenes, vitamin A and vitamin C
270. It prevents peroxidation of unsaturated fatty acids,
and protects the tissues against the harmful
effects of lipid peroxides
It protects the RBC membrane from oxidants, and
makes the RBCs resistant to haemolysis
It protects the pulmonary tissue from atmospheric
oxidants
271. Sources
Vegetable oils, e.g. wheat germ oil, rice bran oil,
corn oil, soya bean oil, cottonseed oil etc, are
very rich in vitamin E
Other good sources are green leafy vegetables,
nuts, legumes, milk, eggs and meat
272. Requirement
Infants : 4-5 IU/day
Children : 7-12 IU/day
Adult men : 15 IU/day
Adult women : 12 IU/day
Pregnant and lactating women : 15 IU/day
273. The requirement of vitamin E is related to the
intake of polyunsaturated fatty acids (PUFA)
It has been suggested that an intake of 0.8 mg of
D-α-tocopherol (or 1.2 IU of vitamin E) per gm of
PUFA in diet will prevent vitamin E deficiency
274. Deficiency
Deficiency of vitamin E is uncommon because of
its widespread distribution in foods
Moreover, the foods which are rich in PUFA, e.g.
vegetable oils, are also rich in vitamin E
275. Deficiency may occur in severely undernourished
children and in premature infants fed on artificial
milk not containing vitamin E
The clinical manifestations of deficiency are
oedema, haemolytic anaemia and thrombocytosis
276. VITAMIN K
Vitamin K activity is present in many compounds
having a 2-methyl-1,4-naphthoquinone nucleus
Two such natural compounds are 2-methyl-3-
phytyl-1,4- naphthoquinone (phylloquinone or
vitamin K1) and 2-methyl-3-difarnesyl-1,4-
naphthoquinone (menaquinone or vitamin K2)
The former occurs in plants and the latter in
bacteria
277. — C H — C H = C — (C H ) — C H — (C H ) — C H — (C H )2 2 3 2 3 2 3 — C H — ( C H )3 2
— C H — ( C H = C — C H ) — C H = C —2 2 5 (C H )3 2
— C H 3
— C H 3
1
2
3
4
C H 3
C H 3
C H 3 C H 3
|
|
| |
2 -M e th y l-3 -p h y ty l-1 ,4 -n a p h th o q u in o n e
2 -M e th y l-3 -d ifa rn e s y l-1 ,4 -n a p h th o q u in o n e
O
O
O
O
278. Some synthetic compounds having vitamin K
activity have also been prepared
These include menadione, sodium menadiol
diphosphate and menadione sodium bisulphite
These three are water-soluble
279. — C H 3
M e n a d io n e
— C H 3
M e n a d io n e s o d iu m b is u lp h ite
S O N a3
|
S o d iu m m e n a d io l d ip h o s p h a te
O N a
O N a
|
|
O — P = O
O — P = O
|
|
O N a
O N a
— C H 3
O O
OO
280. Functions
Vitamin K is essential for normal coagulation of
blood
Concentrations of several coagulation factors in
plasma are decreased in vitamin K deficiency
These include prothrombin (Factor II),
proconvertin (Factor VII), Christmas factor (Factor
IX) and Stuart-Prower Factor (Factor X)
281. All these factors are proteins, and vitamin K is
required for post-translational modification of
these proteins, which is necessary for their
activation
Prothrombin is synthesised in liver in the form of
an inactive precursor, pre-prothrombin
282. Hydroquinone (reduced) form of vitamin K is
required for carboxylation of glutamate residues of
pre-prothrombin as a result of which pre-
prothrombin is converted into prothrombins
During this reaction, hydroquinone form of
vitamin K is converted into 2,3-epoxide form
283. Hydroquinone form is regenerated via quinone
form by 2,3-epoxide reductase and vitamin K
reductase
An as yet unidentified sulphydryl compound is
required in the reaction catalysed by 2,3-epoxide
reductase
284. C O O H
|
C H 2
|
C H 2
|| | |
— N — C H — C —
G l u t a m a t e r e s i d u e
H O
| | |
— N — C H — C —
γ- C a r b o x y g l u t a m a t e r e s i d u e
H O
V i t a m i n K - d e p e n d e n t
c a r b o x y l a s e
H O2
V i t a m i n K
( h y d r o q u i n o n e f o r m )
C O + O2 2
O H
|
— C H 3
— R
|
O H
V i t a m i n K
( 2 , 3 - e p o x i d e f o r m )
O
| |
| |
— C H 3
— R
O
O
V i t a m i n K
r e d u c t a s e
N A D P +
N A D P H + H
+
2 , 3 - E p o x id e r e d u c t a s e
D ic o u m a r o l
R
S H
S H
–
|
C H 2
|
H O O C C O O H
C H
R
S
+ H O2
V i t a m i n K
( q u i n o n e f o r m )
— C H 3
— R
| |
| |
O
O
S
285. At physiological pH, the carboxyl groups attached
to the γ-carbon of carboxyglutamate residues are
ionised
The two negatively charged carboxyl groups act
as a Ca++
-binding site
C O O ––
O O C
C H
|
C H 2
|| | |
— N — C H — C —
H O
286. Thus, the function of vitamin K is to create Ca++
-
binding sites on the prothrombin molecules as a
result of which they become biologically active
Similar Ca++
-binding sites are probably created
by vitamin K on Factors VII, IX and X also
287. Sources
Green leafy vegetables (alfalfa, spinach,
cabbage etc) are rich in vitamin K
Cauliflower and peas are also good sources
Liver is a fair source
Small amounts are present in milk and eggs
also
288. A very important source is bacterial synthesis in
the intestine
The intestinal bacteria synthesise a good deal of
vitamin K which is available to the host
289. Requirement
The exact requirement is not known as sufficient
quantities are synthesised by intestinal bacteria
Vitamin K supplements are required only when
the intestinal bacteria are destroyed or when fat
absorption is impaired
The normal daily requirement is probably less
than 0.1 mg
290. Deficiency
Deficiency of vitamin K leads to a disturbance in
the normal mechanism of coagulation of blood
Even a slight injury causes prolonged bleeding in
a subject deficient in vitamin K
Diagnosis can be made by measuring
prothrombin time which is prolonged in vitamin K
deficiency
291. Prothrombin time may also be prolonged in
patients with liver damage due to inability of the
liver to synthesise prothrombin
These two conditions can be distinguished by
parenteral administration of vitamin K
Prothrombin time returns to normal in subjects
deficient in vitamin K but not in patients with liver
damage