1. DNA structure involves four levels - from nucleotides to chromatin packaging. DNA sequencing methods like Sanger sequencing involve chain termination using dideoxynucleotides.
2. DNA replication requires primers, DNA polymerase and occurs in 5' to 3' direction on each strand.
3. RNA differs from DNA in being single-stranded and more chemically unstable but has important functions as rRNA, tRNA, mRNA and ribozymes.
chemistry of nucleic acids,
history --> Discovered by JOHANN FRIEDRICH MIESCHER
central dogma of life
components of nucleic acids-->Nitrogenous base +pentose sugar +phosphate group.
structure of nucleotides --> purines and pyrimidens
minor bases in nucleic acids are 5-methylcytosine,N4-acetylcytosine, N6-methylsdenine, N6,N6-dimethyladenine, pseudouracil.
Biologically importanat Bases-->Hypoxanthine, Xanthine, uric acid.
Purines bases of plant --> caffeine,theophylline, theobromine
chemistry of nucleic acids,
history --> Discovered by JOHANN FRIEDRICH MIESCHER
central dogma of life
components of nucleic acids-->Nitrogenous base +pentose sugar +phosphate group.
structure of nucleotides --> purines and pyrimidens
minor bases in nucleic acids are 5-methylcytosine,N4-acetylcytosine, N6-methylsdenine, N6,N6-dimethyladenine, pseudouracil.
Biologically importanat Bases-->Hypoxanthine, Xanthine, uric acid.
Purines bases of plant --> caffeine,theophylline, theobromine
This power point presentation explains double helical structure of DNA as proposed by Watson and Crick (1953).Attempts have also been made to high light the valuable contributions made by Rosalind Franklin and Wilkins. Brief details of different types of DNA have also been included.
There are slides about DNA replication and types of DNA.
Here we study about different enzymes of replication and its process.Places of enzyme action also shown in the slides.Different proteins are also discussed.
Replication Introduction , DNA replicating Models , Meselson and Stahl Experiments , Circuler Model of DNA replication , Replication in Prokaryotes , Replication In Eukaryotes , Comparison Between Prokaryotes and Eukaryotes Replicaton and PCR (Polymerease Chain Reaction)
it describes transcription with simple diagram and animation. its steps and inhibitors are described for both eukaryotes and prokaryotes. it will be easily understood by UG students . post transcriptional modification of all the RNA are also described with diagrams.
The base sequence information present in the gene (DNA) is copied into an RNA molecule, which directly participates in protein synthesis and provides information for amino acid sequence of the protein. This RNA molecule is called messenger RNA or mRNA. The process of production of RNA copy of a DNA sequence is called transcription; this reaction is catalyzed by DNA-directed RNA polymerase, or simply RNA polymerase.
This power point presentation explains double helical structure of DNA as proposed by Watson and Crick (1953).Attempts have also been made to high light the valuable contributions made by Rosalind Franklin and Wilkins. Brief details of different types of DNA have also been included.
There are slides about DNA replication and types of DNA.
Here we study about different enzymes of replication and its process.Places of enzyme action also shown in the slides.Different proteins are also discussed.
Replication Introduction , DNA replicating Models , Meselson and Stahl Experiments , Circuler Model of DNA replication , Replication in Prokaryotes , Replication In Eukaryotes , Comparison Between Prokaryotes and Eukaryotes Replicaton and PCR (Polymerease Chain Reaction)
it describes transcription with simple diagram and animation. its steps and inhibitors are described for both eukaryotes and prokaryotes. it will be easily understood by UG students . post transcriptional modification of all the RNA are also described with diagrams.
The base sequence information present in the gene (DNA) is copied into an RNA molecule, which directly participates in protein synthesis and provides information for amino acid sequence of the protein. This RNA molecule is called messenger RNA or mRNA. The process of production of RNA copy of a DNA sequence is called transcription; this reaction is catalyzed by DNA-directed RNA polymerase, or simply RNA polymerase.
Domains were added to Node.js in 0.8, but their use and workings have been a relative mystery. In short, domains are a structured way of reacting to uncaught exceptions; for example, when creating an HTTP server, you can use domains to send 500 errors when exceptions occur instead of crashing your server. This talk will go over what domains are, how to use them, and some of the subtleties behind how they work.
Ramachandran plot for structural validation of protein will give information whether your protein or model protein is allowed or not in three dimensional point of view.
Describes the structural organisation of proteins with example and its determination, interrelationship b/w structure and function of proteins, also biologically important peptides is covered.
by Dr. N. Sivaranjani, MD
Dna chemistry structure,fuctions and its orgainizationneha sheth
DNA, Nucleotides, Structure of DNA, Features of DNA, watson-crick Model of DNA,base pairing rule, Denaturation of DNA,Higher organization of DNA, Histones, chromosomes, nucleosomes, introns, extrons, repeat sequences of DNA
Nucleic Acids
DNA
Eukaryotic Chromosomes
The Histones
Deoxynucleic acid ( DNA )
Importance of Nucleotides
Base pairing
Denaturation and Renaturation
Determination GC content
Prokaryotic DNA synthesis
Prokaryotic DNA Replication
Transcription
Coding Strand and Template Strand
Steps of RNA synthesize
DNA replication is the process by which DNA makes a copy of itself during cell division.The separation of the two single strands of DNA creates a 'Y' shape called a replication 'fork'. The two separated strands will act as templates for making the new strands of DNA.
This is a presentation by Dada Robert in a Your Skill Boost masterclass organised by the Excellence Foundation for South Sudan (EFSS) on Saturday, the 25th and Sunday, the 26th of May 2024.
He discussed the concept of quality improvement, emphasizing its applicability to various aspects of life, including personal, project, and program improvements. He defined quality as doing the right thing at the right time in the right way to achieve the best possible results and discussed the concept of the "gap" between what we know and what we do, and how this gap represents the areas we need to improve. He explained the scientific approach to quality improvement, which involves systematic performance analysis, testing and learning, and implementing change ideas. He also highlighted the importance of client focus and a team approach to quality improvement.
Read| The latest issue of The Challenger is here! We are thrilled to announce that our school paper has qualified for the NATIONAL SCHOOLS PRESS CONFERENCE (NSPC) 2024. Thank you for your unwavering support and trust. Dive into the stories that made us stand out!
We all have good and bad thoughts from time to time and situation to situation. We are bombarded daily with spiraling thoughts(both negative and positive) creating all-consuming feel , making us difficult to manage with associated suffering. Good thoughts are like our Mob Signal (Positive thought) amidst noise(negative thought) in the atmosphere. Negative thoughts like noise outweigh positive thoughts. These thoughts often create unwanted confusion, trouble, stress and frustration in our mind as well as chaos in our physical world. Negative thoughts are also known as “distorted thinking”.
Operation “Blue Star” is the only event in the history of Independent India where the state went into war with its own people. Even after about 40 years it is not clear if it was culmination of states anger over people of the region, a political game of power or start of dictatorial chapter in the democratic setup.
The people of Punjab felt alienated from main stream due to denial of their just demands during a long democratic struggle since independence. As it happen all over the word, it led to militant struggle with great loss of lives of military, police and civilian personnel. Killing of Indira Gandhi and massacre of innocent Sikhs in Delhi and other India cities was also associated with this movement.
How to Make a Field invisible in Odoo 17Celine George
It is possible to hide or invisible some fields in odoo. Commonly using “invisible” attribute in the field definition to invisible the fields. This slide will show how to make a field invisible in odoo 17.
Synthetic Fiber Construction in lab .pptxPavel ( NSTU)
Synthetic fiber production is a fascinating and complex field that blends chemistry, engineering, and environmental science. By understanding these aspects, students can gain a comprehensive view of synthetic fiber production, its impact on society and the environment, and the potential for future innovations. Synthetic fibers play a crucial role in modern society, impacting various aspects of daily life, industry, and the environment. ynthetic fibers are integral to modern life, offering a range of benefits from cost-effectiveness and versatility to innovative applications and performance characteristics. While they pose environmental challenges, ongoing research and development aim to create more sustainable and eco-friendly alternatives. Understanding the importance of synthetic fibers helps in appreciating their role in the economy, industry, and daily life, while also emphasizing the need for sustainable practices and innovation.
The Art Pastor's Guide to Sabbath | Steve ThomasonSteve Thomason
What is the purpose of the Sabbath Law in the Torah. It is interesting to compare how the context of the law shifts from Exodus to Deuteronomy. Who gets to rest, and why?
2024.06.01 Introducing a competency framework for languag learning materials ...Sandy Millin
http://sandymillin.wordpress.com/iateflwebinar2024
Published classroom materials form the basis of syllabuses, drive teacher professional development, and have a potentially huge influence on learners, teachers and education systems. All teachers also create their own materials, whether a few sentences on a blackboard, a highly-structured fully-realised online course, or anything in between. Despite this, the knowledge and skills needed to create effective language learning materials are rarely part of teacher training, and are mostly learnt by trial and error.
Knowledge and skills frameworks, generally called competency frameworks, for ELT teachers, trainers and managers have existed for a few years now. However, until I created one for my MA dissertation, there wasn’t one drawing together what we need to know and do to be able to effectively produce language learning materials.
This webinar will introduce you to my framework, highlighting the key competencies I identified from my research. It will also show how anybody involved in language teaching (any language, not just English!), teacher training, managing schools or developing language learning materials can benefit from using the framework.
Model Attribute Check Company Auto PropertyCeline George
In Odoo, the multi-company feature allows you to manage multiple companies within a single Odoo database instance. Each company can have its own configurations while still sharing common resources such as products, customers, and suppliers.
3.
1o Structure - Linear array of nucleotides
2o Structure – double helix
3o Structure - Super-coiling, stem-loop
formation
4o Structure – Packaging into chromatin
4.
Can determine the sequence of DNA base pairs in
any DNA molecule
Chain-termination method developed by Sanger
Involves in vitro replication of target DNA
Technology led to the sequencing of the human
genome
5. DNA Replication
• DNA is a double-helical molecule
• Each strand of the helix must be copied in
complementary fashion by DNA polymerase
• Each strand is a template for copying
• DNA polymerase requires template and
primer
6.
Primer: an oligonucleotide that pairs with
the end of the template molecule to form
dsDNA
DNA polymerases add nucleotides in 5'-3'
direction
7.
8. Chain Termination Method
• Based on DNA polymerase reaction
• 4 separate rxns
• Each reaction mixture contains dATP, dGTP,
dCTP and dTTP
9.
Each reaction also contains a small amount of
one dideoxynucleotide (ddATP, ddGTP, ddCTP
and ddTTP).
Each of the 4 dideoxynucleotides are labeled
with a different fluorescent dye.
Dideoxynucleotides missing 3’-OH group. Once
incorporated into the DNA chain, chain
elongation stops)
10. Chain Termination Method
• Most of the time, the polymerase uses
normal nucleotides and DNA molecules grow
normally
• Occasionally, the polymerase uses a
dideoxynucleotide, which adds to the chain
and then prevents further growth in that
molecule
11.
Random insertion of dd-nucleotides leaves
(optimally) at least a few chains terminated
at every occurrence of a given nucleotide
14. Chain Termination Method
• Run each reaction mixture on electrophoresis gel
• Short fragments go to bottom, long fragments on
top
• Read the "sequence" from bottom of gel to top
• Convert this "sequence" to the complementary
sequence
• Now read from the other end and you have the
sequence you wanted - read 5' to 3'
15.
16.
17.
DNA is double stranded with antiparallel
strands
Right hand double helix
Three different helical forms (A, B and Z
DNA.
18. Comparison of A, B, Z DNA
• A: right-handed, short and broad, 2.3 A, 11
bp per turn
• B: right-handed, longer, thinner, 3.32 A, 10
bp per turn
• Z: left-handed, longest, thinnest, 3.8 A, 12
bp per turn
20. Z-DNA
• Found in G:C-rich
regions of DNA
• G goes to syn
conformation
• C stays anti but
whole C nucleoside
(base and sugar)
flips 180 degrees
21.
Double Strand DNA can be denatured by
heat (get strand separation)
Can determine degree of denturation by
measuring absorbance at 260 nm.
22.
Conjugated double bonds in bases absorb
light at 260 nm.
Base stacking causes less absorbance.
Increased single strandedness causes
increase in absorbance
23.
24.
Melting temperature related to G:C and
A:T content.
3 H-bonds of G:C pair require higher
temperatures to denture than 2 H-bonds of
A:T pair.
27. Supercoils
• In duplex DNA, ten bp per turn of helix
(relaxed form)
• DNA helix can be over-wound.
• Over winding of DNA helix can be
compensated by supercoiling
28.
Supercoiling prevalent in circular DNA
molecules and within local regions of long
linear DNA strands
Enzymes called topoisomerases or gyrases can
introduce or remove supercoils
In vivo most DNA is negatively supercoiled.
Therefore, it is easy to unwind short regions of
the molecule to allow access for enzymes
29. Each super coil compensates for one + or –
turn of the double helix
30. Cruciforms occur in palindromic regions
of DNA
Can form intrachain base pairing
Negative supercoiling may promote
cruciforms
34. Chromosome Structure
• Human DNA’s total length is ~2 meters!
• This must be packaged into a nucleus that is
about 5 micrometers in diameter
• This represents a compression of more than
100,000!
35.
It is made possible by wrapping the DNA
around protein spools called nucleosomes
and then packing these in helical filaments
36. Nucleosome Structure
• Chromatin, the nucleoprotein complex,
consists of histones and nonhistone
chromosomal proteins
• % major histone proteins: H1, H2A, H2B,
H3 and H4
37.
Histone octamers are major part of the
“protein spools”
Nonhistone proteins are regulators of gene
expression
38. •4 major histone (H2A, H2B, H3, H4) proteins for
octomer
•200 base pair long DNA strand winds around the
octomer
•146 base pair DNA “spacer separates individual
nucleosomes
•H1 protein involved in higher-order chromatin
structure.
•W/O H1, Chromatin looks like beads on string
42.
Single stranded molecule
Chemically less stable than DNA
presence of 2’-OH makes RNA more
susceptible to hydrolytic attack (especially form
bases)
Prone to degradation by Ribonucleases (Rnases)
43.
Has secondary structure. Can form
intrachain base pairing (i.e.cruciform
structures).
Multiple functions
44.
Ribosomal RNA (rRNA) – integral part of
ribosomes (very abundant)
Transfer RNA (tRNA) – carries activated amino
acids to ribosomes.
45.
Messenger RNA (mRNA) – endcodes
sequences of amino acids in proteins.
Catalytic RNA (Ribozymes) – catalzye
cleavage of specific RNA species.