This document provides information on Group B Streptococcus (GBS) and Enterococcus. For GBS, it discusses the pathogenisis, including virulence factors like the polysaccharide capsule and beta-hemolysin. It also covers epidemiology, risk factors, clinical manifestations of early vs late onset disease, and the gold standard test for diagnosis. For Enterococcus, it introduces the two common species, describes increasing importance as nosocomial pathogens and vancomycin resistance, and lists common sites of infection.
a brief description on morphology and pathogenesis caused by Staphylococcus Aureus, Staphylococcus Epidermidis, Staphylococcus Saprophyticus, toxic shock syndrome toxin (tsst) and it's treatment, enterotoxin , exfoliatin , pyogenic infections, abscess etc
contact me via my email: maryamhy95@gmail.com
a brief description on morphology and pathogenesis caused by Staphylococcus Aureus, Staphylococcus Epidermidis, Staphylococcus Saprophyticus, toxic shock syndrome toxin (tsst) and it's treatment, enterotoxin , exfoliatin , pyogenic infections, abscess etc
contact me via my email: maryamhy95@gmail.com
teaching support for 2nd year medical school students: steps of the laboratory diagnosis of infections caused by bacteria of the genera Staphylococcus and Streptococcus
In this presentation, we delve into the realm of opportunistic gastrointestinal pathogens, shedding light on the often underestimated threats they pose to human health. These microorganisms, while typically harmless in healthy individuals, can turn perilous in situations where the host's immune system is compromised or when environmental conditions become conducive to their proliferation. We will explore the factors that render individuals susceptible to these infections, ranging from immunosuppression to suboptimal hygiene practices. By understanding the key players in opportunistic infections, including notorious culprits like Clostridium difficile, Candida species, Norovirus, and Giardia, we aim to equip our audience with valuable insights for prevention and management strategies. Join us in this journey as we unmask the covert adversaries within our gastrointestinal tract.
Pulmonary Thromboembolism - etilogy, types, medical- Surgical and nursing man...VarunMahajani
Disruption of blood supply to lung alveoli due to blockage of one or more pulmonary blood vessels is called as Pulmonary thromboembolism. In this presentation we will discuss its causes, types and its management in depth.
The prostate is an exocrine gland of the male mammalian reproductive system
It is a walnut-sized gland that forms part of the male reproductive system and is located in front of the rectum and just below the urinary bladder
Function is to store and secrete a clear, slightly alkaline fluid that constitutes 10-30% of the volume of the seminal fluid that along with the spermatozoa, constitutes semen
A healthy human prostate measures (4cm-vertical, by 3cm-horizontal, 2cm ant-post ).
It surrounds the urethra just below the urinary bladder. It has anterior, median, posterior and two lateral lobes
It’s work is regulated by androgens which are responsible for male sex characteristics
Generalised disease of the prostate due to hormonal derangement which leads to non malignant enlargement of the gland (increase in the number of epithelial cells and stromal tissue)to cause compression of the urethra leading to symptoms (LUTS
Acute scrotum is a general term referring to an emergency condition affecting the contents or the wall of the scrotum.
There are a number of conditions that present acutely, predominantly with pain and/or swelling
A careful and detailed history and examination, and in some cases, investigations allow differentiation between these diagnoses. A prompt diagnosis is essential as the patient may require urgent surgical intervention
Testicular torsion refers to twisting of the spermatic cord, causing ischaemia of the testicle.
Testicular torsion results from inadequate fixation of the testis to the tunica vaginalis producing ischemia from reduced arterial inflow and venous outflow obstruction.
The prevalence of testicular torsion in adult patients hospitalized with acute scrotal pain is approximately 25 to 50 percent
Report Back from SGO 2024: What’s the Latest in Cervical Cancer?bkling
Are you curious about what’s new in cervical cancer research or unsure what the findings mean? Join Dr. Emily Ko, a gynecologic oncologist at Penn Medicine, to learn about the latest updates from the Society of Gynecologic Oncology (SGO) 2024 Annual Meeting on Women’s Cancer. Dr. Ko will discuss what the research presented at the conference means for you and answer your questions about the new developments.
Lung Cancer: Artificial Intelligence, Synergetics, Complex System Analysis, S...Oleg Kshivets
RESULTS: Overall life span (LS) was 2252.1±1742.5 days and cumulative 5-year survival (5YS) reached 73.2%, 10 years – 64.8%, 20 years – 42.5%. 513 LCP lived more than 5 years (LS=3124.6±1525.6 days), 148 LCP – more than 10 years (LS=5054.4±1504.1 days).199 LCP died because of LC (LS=562.7±374.5 days). 5YS of LCP after bi/lobectomies was significantly superior in comparison with LCP after pneumonectomies (78.1% vs.63.7%, P=0.00001 by log-rank test). AT significantly improved 5YS (66.3% vs. 34.8%) (P=0.00000 by log-rank test) only for LCP with N1-2. Cox modeling displayed that 5YS of LCP significantly depended on: phase transition (PT) early-invasive LC in terms of synergetics, PT N0—N12, cell ratio factors (ratio between cancer cells- CC and blood cells subpopulations), G1-3, histology, glucose, AT, blood cell circuit, prothrombin index, heparin tolerance, recalcification time (P=0.000-0.038). Neural networks, genetic algorithm selection and bootstrap simulation revealed relationships between 5YS and PT early-invasive LC (rank=1), PT N0—N12 (rank=2), thrombocytes/CC (3), erythrocytes/CC (4), eosinophils/CC (5), healthy cells/CC (6), lymphocytes/CC (7), segmented neutrophils/CC (8), stick neutrophils/CC (9), monocytes/CC (10); leucocytes/CC (11). Correct prediction of 5YS was 100% by neural networks computing (area under ROC curve=1.0; error=0.0).
CONCLUSIONS: 5YS of LCP after radical procedures significantly depended on: 1) PT early-invasive cancer; 2) PT N0--N12; 3) cell ratio factors; 4) blood cell circuit; 5) biochemical factors; 6) hemostasis system; 7) AT; 8) LC characteristics; 9) LC cell dynamics; 10) surgery type: lobectomy/pneumonectomy; 11) anthropometric data. Optimal diagnosis and treatment strategies for LC are: 1) screening and early detection of LC; 2) availability of experienced thoracic surgeons because of complexity of radical procedures; 3) aggressive en block surgery and adequate lymph node dissection for completeness; 4) precise prediction; 5) adjuvant chemoimmunoradiotherapy for LCP with unfavorable prognosis.
These simplified slides by Dr. Sidra Arshad present an overview of the non-respiratory functions of the respiratory tract.
Learning objectives:
1. Enlist the non-respiratory functions of the respiratory tract
2. Briefly explain how these functions are carried out
3. Discuss the significance of dead space
4. Differentiate between minute ventilation and alveolar ventilation
5. Describe the cough and sneeze reflexes
Study Resources:
1. Chapter 39, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 34, Ganong’s Review of Medical Physiology, 26th edition
3. Chapter 17, Human Physiology by Lauralee Sherwood, 9th edition
4. Non-respiratory functions of the lungs https://academic.oup.com/bjaed/article/13/3/98/278874
Explore natural remedies for syphilis treatment in Singapore. Discover alternative therapies, herbal remedies, and lifestyle changes that may complement conventional treatments. Learn about holistic approaches to managing syphilis symptoms and supporting overall health.
New Directions in Targeted Therapeutic Approaches for Older Adults With Mantl...i3 Health
i3 Health is pleased to make the speaker slides from this activity available for use as a non-accredited self-study or teaching resource.
This slide deck presented by Dr. Kami Maddocks, Professor-Clinical in the Division of Hematology and
Associate Division Director for Ambulatory Operations
The Ohio State University Comprehensive Cancer Center, will provide insight into new directions in targeted therapeutic approaches for older adults with mantle cell lymphoma.
STATEMENT OF NEED
Mantle cell lymphoma (MCL) is a rare, aggressive B-cell non-Hodgkin lymphoma (NHL) accounting for 5% to 7% of all lymphomas. Its prognosis ranges from indolent disease that does not require treatment for years to very aggressive disease, which is associated with poor survival (Silkenstedt et al, 2021). Typically, MCL is diagnosed at advanced stage and in older patients who cannot tolerate intensive therapy (NCCN, 2022). Although recent advances have slightly increased remission rates, recurrence and relapse remain very common, leading to a median overall survival between 3 and 6 years (LLS, 2021). Though there are several effective options, progress is still needed towards establishing an accepted frontline approach for MCL (Castellino et al, 2022). Treatment selection and management of MCL are complicated by the heterogeneity of prognosis, advanced age and comorbidities of patients, and lack of an established standard approach for treatment, making it vital that clinicians be familiar with the latest research and advances in this area. In this activity chaired by Michael Wang, MD, Professor in the Department of Lymphoma & Myeloma at MD Anderson Cancer Center, expert faculty will discuss prognostic factors informing treatment, the promising results of recent trials in new therapeutic approaches, and the implications of treatment resistance in therapeutic selection for MCL.
Target Audience
Hematology/oncology fellows, attending faculty, and other health care professionals involved in the treatment of patients with mantle cell lymphoma (MCL).
Learning Objectives
1.) Identify clinical and biological prognostic factors that can guide treatment decision making for older adults with MCL
2.) Evaluate emerging data on targeted therapeutic approaches for treatment-naive and relapsed/refractory MCL and their applicability to older adults
3.) Assess mechanisms of resistance to targeted therapies for MCL and their implications for treatment selection
Flu Vaccine Alert in Bangalore Karnatakaaddon Scans
As flu season approaches, health officials in Bangalore, Karnataka, are urging residents to get their flu vaccinations. The seasonal flu, while common, can lead to severe health complications, particularly for vulnerable populations such as young children, the elderly, and those with underlying health conditions.
Dr. Vidisha Kumari, a leading epidemiologist in Bangalore, emphasizes the importance of getting vaccinated. "The flu vaccine is our best defense against the influenza virus. It not only protects individuals but also helps prevent the spread of the virus in our communities," he says.
This year, the flu season is expected to coincide with a potential increase in other respiratory illnesses. The Karnataka Health Department has launched an awareness campaign highlighting the significance of flu vaccinations. They have set up multiple vaccination centers across Bangalore, making it convenient for residents to receive their shots.
To encourage widespread vaccination, the government is also collaborating with local schools, workplaces, and community centers to facilitate vaccination drives. Special attention is being given to ensuring that the vaccine is accessible to all, including marginalized communities who may have limited access to healthcare.
Residents are reminded that the flu vaccine is safe and effective. Common side effects are mild and may include soreness at the injection site, mild fever, or muscle aches. These side effects are generally short-lived and far less severe than the flu itself.
Healthcare providers are also stressing the importance of continuing COVID-19 precautions. Wearing masks, practicing good hand hygiene, and maintaining social distancing are still crucial, especially in crowded places.
Protect yourself and your loved ones by getting vaccinated. Together, we can help keep Bangalore healthy and safe this flu season. For more information on vaccination centers and schedules, residents can visit the Karnataka Health Department’s official website or follow their social media pages.
Stay informed, stay safe, and get your flu shot today!
micro teaching on communication m.sc nursing.pdfAnurag Sharma
Microteaching is a unique model of practice teaching. It is a viable instrument for the. desired change in the teaching behavior or the behavior potential which, in specified types of real. classroom situations, tends to facilitate the achievement of specified types of objectives.
Tom Selleck Health: A Comprehensive Look at the Iconic Actor’s Wellness Journeygreendigital
Tom Selleck, an enduring figure in Hollywood. has captivated audiences for decades with his rugged charm, iconic moustache. and memorable roles in television and film. From his breakout role as Thomas Magnum in Magnum P.I. to his current portrayal of Frank Reagan in Blue Bloods. Selleck's career has spanned over 50 years. But beyond his professional achievements. fans have often been curious about Tom Selleck Health. especially as he has aged in the public eye.
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Introduction
Many have been interested in Tom Selleck health. not only because of his enduring presence on screen but also because of the challenges. and lifestyle choices he has faced and made over the years. This article delves into the various aspects of Tom Selleck health. exploring his fitness regimen, diet, mental health. and the challenges he has encountered as he ages. We'll look at how he maintains his well-being. the health issues he has faced, and his approach to ageing .
Early Life and Career
Childhood and Athletic Beginnings
Tom Selleck was born on January 29, 1945, in Detroit, Michigan, and grew up in Sherman Oaks, California. From an early age, he was involved in sports, particularly basketball. which played a significant role in his physical development. His athletic pursuits continued into college. where he attended the University of Southern California (USC) on a basketball scholarship. This early involvement in sports laid a strong foundation for his physical health and disciplined lifestyle.
Transition to Acting
Selleck's transition from an athlete to an actor came with its physical demands. His first significant role in "Magnum P.I." required him to perform various stunts and maintain a fit appearance. This role, which he played from 1980 to 1988. necessitated a rigorous fitness routine to meet the show's demands. setting the stage for his long-term commitment to health and wellness.
Fitness Regimen
Workout Routine
Tom Selleck health and fitness regimen has evolved. adapting to his changing roles and age. During his "Magnum, P.I." days. Selleck's workouts were intense and focused on building and maintaining muscle mass. His routine included weightlifting, cardiovascular exercises. and specific training for the stunts he performed on the show.
Selleck adjusted his fitness routine as he aged to suit his body's needs. Today, his workouts focus on maintaining flexibility, strength, and cardiovascular health. He incorporates low-impact exercises such as swimming, walking, and light weightlifting. This balanced approach helps him stay fit without putting undue strain on his joints and muscles.
Importance of Flexibility and Mobility
In recent years, Selleck has emphasized the importance of flexibility and mobility in his fitness regimen. Understanding the natural decline in muscle mass and joint flexibility with age. he includes stretching and yoga in his routine. These practices help prevent injuries, improve posture, and maintain mobilit
These lecture slides, by Dr Sidra Arshad, offer a quick overview of physiological basis of a normal electrocardiogram.
Learning objectives:
1. Define an electrocardiogram (ECG) and electrocardiography
2. Describe how dipoles generated by the heart produce the waveforms of the ECG
3. Describe the components of a normal electrocardiogram of a typical bipolar leads (limb II)
4. Differentiate between intervals and segments
5. Enlist some common indications for obtaining an ECG
Study Resources:
1. Chapter 11, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 9, Human Physiology - From Cells to Systems, Lauralee Sherwood, 9th edition
3. Chapter 29, Ganong’s Review of Medical Physiology, 26th edition
4. Electrocardiogram, StatPearls - https://www.ncbi.nlm.nih.gov/books/NBK549803/
5. ECG in Medical Practice by ABM Abdullah, 4th edition
6. ECG Basics, http://www.nataliescasebook.com/tag/e-c-g-basics
New Drug Discovery and Development .....NEHA GUPTA
The "New Drug Discovery and Development" process involves the identification, design, testing, and manufacturing of novel pharmaceutical compounds with the aim of introducing new and improved treatments for various medical conditions. This comprehensive endeavor encompasses various stages, including target identification, preclinical studies, clinical trials, regulatory approval, and post-market surveillance. It involves multidisciplinary collaboration among scientists, researchers, clinicians, regulatory experts, and pharmaceutical companies to bring innovative therapies to market and address unmet medical needs.
4.
Introduction
Gram-positive cocci
Chains
Encapsulated
Non-motile
Facultative anaerobes
Lactic acid production
Multiple nutritional
requirements
1-4 mm diameter, grey-
white, flat, mucoid
Selective Broth Media
(SBM) or Lim broth
4
5.
5
• Divided into the following serotypes based on
capsular polysacchride. types Ia, Ib,II and III
through VII.
• All serotypes can cause infections in newborns
but Ia,II,III,V account for 90%.
• Late onset dx and early-onset meningitis is due
to type III.
6.
6
β-hemolysis
RBCs surrounding the colony are completely lysed
“Hemolysin" toxins
Narrow hemolysis zones
CAMP factor enhances hemolysis
Carbohydrate Antigens (C substance)
Lancefield Group B
Group specific antigen
Polysaccharide Capsule Serotypes
150 oligosaccharide subunits with mono-, di-, tri-
side chains
Ia, Ib, II-VIII
III and V completely sequenced
Classification
7.
7
Epidemiology
Approx. 10%-35% of pregnant women are
asymptomatic carriers of GBS in the genital and
Gastro Intestinal tract.
At birth 1 in 2 infants born to colonized mothers
are colonized.
98% of colonized infants are without symptoms,
but 1%-2% developed GBS.
Nearly 50% of sexual partners of colonized
women are colonized themselves.
8.
8
• Incidence rate of 0.2 – 3.7/1000 live births.
• Mortality rate of 5-15/1000 live births.
• More recent surveillance shows a decrease in
Incident rate to 0.8 per 1000 live births-reflection of
use of maternal antibiotic prophylaxis
• Direct cost of treating neonate with proven GBS –
300 million dollars/year.
9.
9
Virulence Factor
GBS Surface Polysaccharide Capsule
Antiphagocytic properties
Sialic acid residues on capsule inhibit the binding
of active C3 component of complement to the cell
surface
blocking activation of the alternative
pathway
Transplacental passage of type-specific anti-
capsular IgG antibody from mother to infant is an
important protective factor against invasive disease
10.
10
GBS β-hemolysin
Cytotoxic to pulmonary epithelial and endothelial
cells
Pulmonary injury and alveolar protein exudate in
early-onset pneumonia
Activity is blocked by surfactant phospholipid
Increased risk of premature, surfactant-deficient
neonates for severe pneumonia
Induces cytokine release and nitric oxide
production in macrophages
Stimulate elements of the sepsis cascade
11.
11
C5a-peptidase
Cleaves and inactivates the complement-derived
neutrophil chemoattractant C5a
C5a-peptidase-deficient mutants are more
rapidly cleared from the lungs of infected
animals when compared to the isogenic wild-
type strain
13.
13
What does group B Streptococcus do?
Colonisation
Asymptomatic and
intermittent
Intestinal (<30% of
adults)
Vaginal (<25% of women)
Infection
Newborn babies
Adults: the elderly,
pregnant/postpartum
women, others with
underlying disease
IN MOTHER
LOWER GENITAL
TRACT
ANORECTUM
URINARY TRACT
IN NEONATES
EXTERNAL EAR (IN
FIRST 24 H )
ANTERIOR NARES,
THROAT
ANORECTUM
UMBILICUS
14.
14
Risk Factors for Colonization
Heavily colonized mothers
Mothers younger than 20
African Americans
Lower socioeconomic groups
PROM
Prolonged labor
Maternal Chorioamnionitis
Previous delivery with GBS disease
15.
15
Early onset vs. Late onset
Occurs in 1st week.
Usually before 72hrs
Pathophysiology.
-Colonization.
-Immature host
defense mechanism
particularly among
low birth weight
infants.
1week to 6months.
Usually at 3-4 weeks.
Pathophysiology.
- Alteration of the
mucosa barrier by a
viral respiratory tract
infection.
- weakened host
defense
- decrease amount of
maternal antibodies.
16.
16
Early onset vs. Late onset
Transmission:
- aquired through
vertical transmission.
- during passage
through a colonized
birth canal.
Transmission:
- aquired through
horizontal transmission:
-nurseries
-hospital personnel
-community
17.
17
Early onset vs. Late onset
Clinical Manifestation
- Pneumonia
- respiratory distress
- cyanosis,
- hypoxaemia
- apnea
- Shock
- Poor feeding
-Less often meningitis
- Abnormal temprature
Clinical
manifestation:
Occult bacteremia
Meningitis
Ventriculitis
other focal
infections, e.g.
septic arthritis,
osteomyelitis.
19.
19
Presumptive
identification tests
Bile-esculin-
hydrolysis–
negative
Does not grow in
6.5% NaCl
CAMP-test–
positive
Hippurate test-
positive
Latex
agglutination test
20.
20
“Gold Standard” test for GBS carriage
When?
35-37 weeks of pregnancy
Where & who?
Low vagina & anorectal swab/s (no speculum)
Health care professional or pregnant woman
What culture method?
Enriched Culture Medium (ECM) - 24-48 hours to
grow
HPA BSOP58 Processing Swabs for GBS carriage
21.
Pigmented Broth — Positive Result
Positive color change
Photo courtesy of Dr. Lesley McGee, CDC
Inoculate into enrichment broth
Non-pigmented broth
Lim broth, TransVag broth
TransVag broth should be
supplemented with 5%
defibrinated sheep blood
OR
Pigmented broth
StrepB carrot broth, Granada
biphasic broth
22.
22
Antibiotic Recommendations —Intrapartum
Prophylaxis
Standard: Penicillin (PCN) or ampicillin
Alternatives:
• PCN-allergic and low risk for anaphylaxis:
cefazolin
• PCN-allergic but high risk for anaphylaxis
depends on susceptibility to clindamycin &
erythromycin
– If susceptible to clindamycin (including lack of
inducible resistance) clindamycin
– If unknown or not susceptiblevancomycin
23.
Introduction to Enterococci
Enterococci are gram-
positive cocci which
often occur in pairs
(diplococci)
Two species are common
commensal organisms in
the intestines of humans:
E. faecalis and E.
faecium
23
24.
Characters of Enterococci
Gram(+) ,
Catalase(-) Cocci
Can grow in media :
6.5% sodium
chloride
E. faecalis and E.
faecium (90%)
Part of the normal
bowel flora. the
prominent cause of
nosocomial
infections.
24
25.
Habitat of Enterococci
Enterococci normally inhabit
the bowel.
They are found in the
intestine of nearly all animals,
from cockroaches to humans.
Enterococci are readily
recovered outdoors from
vegetation and surface water
In humans, typical
concentrations of enterococci
in stool are up to 108 CFU per
gram .
25
26. normal flora of the intestinal tract.
Enterococcus faecalis frequently causes infections
within the peritoneal cavity
especially following penetrating trauma such as
gunshot wounds and surgical wounds
urinary tract infections
prostate infections
infections of damaged or compromised skin, such
as diabetic or decubitus ulcers, burns, and
surgical wounds.
Other opportunistic fecal streptococci include E.
faecium and E. durans.
Growing Importance of Enterococci
26
27.
Prominent Cause of Nosocomial Infections
The enterococci have
become the second most
common bacterium
isolated from nosocomial
urinary and wound
infections, and the third
most common cause of
nosocomial bacteremia.
Furthermore, the
enterococci are among the
most antibiotic resistant of
all bacteria, with some
isolates resistant to all
known antibiotics
27
28.
Clinical Manifestation
Infections with VRE do not
differ from other
enterococcal infections
other than in their therapy.
The most common sites of
infection :
The urinary tract and
bloodstream.
In addition, enterococci
may cause endocarditis
due to their ability to
adhere to heart valves.
They rarely cause
respiratory tract infections. 28
31.
Glycopeptides
Mechanism of Action
Vancomycin and
teicoplanin inhibit cell wall
synthesis by forming
complexes with peptidyl-D-
alanyl-D-alanine termininal
vanA and vanB resistance
phenotypes are associated
with the acquisition of
gene clusters that lead to
the production of
peptidoglycan ending in D-
alanyl-D-lactate 31
32.
Vancomycin-resistant enterococci (VRE), first
reported in Europe in 1988, are emerging as a
global threat to public health .
The incidence of VRE infection and colonization
among hospitalized patients has increased rapidly
in the last few years.
From 1989, the year VRE was first identified in the
United States, through 1993. Infection with VRE
may be associated with increased mortality , and
no effective antimicrobial therapy is available for
many VRE .
Vancomycin Resistance Increases Morbidity
and Mortality
32
33.
VRE Epidemiology
Found world-wide, but rates vary greatly
Hospital outbreaks often involve clonal spread
Also seen in nursing homes and long term care
facilities
First described in Europe
Primarily a nosocomial pathogen
Alarming increase from 1989 to 1993
intensive care units & teaching hospitals
33
34. vanA and vanB Phenotypes
vanA vanB
Vancomycin MIC >64 4-1024
Teicoplanin MIC 16-512 0.5
Usual species faecium, faecalis faecium, faecalis
Acquired Yes Yes
Transferable Yes Yes
35. vanC, vanD, and vanE Phenotypes
vanC vanD vanE
Vancomycin
MIC
2-32 128 16
Teicoplanin
MIC
0.5 4.0 0.5
Usual species gallinarum,
casseliflavis,
flavescens
faecium faecalis
Acquired No Yes Yes
Transferable No No No
37.
Result
Spreading Resistance
Enterococci that
acquire the vanA
phenotype are highly
resistant to
vancomycin and to
teicoplanin
Enterococci can pass
the vanA gene cluster
to S. aureus
E. faecalis rather
tan E. faecium (so
far) 37
38. Urinary tract infection (most common)
Intra-abdominal and pelvic infection (also
common)
Surgical wound infection
Bacteremia—bacteria in the blood
Endocarditis —infection of the inner surface of
the heart muscles and valves
Neonatal sepsis —bacteria in the blood,
occurring in infants
Meningitis —infection of the membranes that
surround the brain and spinal cord
Infections Caused by Vancomycin Resistant
Enterococci
38
40.
Detection of Vancomycin Resistance
Susceptibility to
vancomycin can be
performed by Kirby-Bauer
Disc Diffusion Method on
Mueller Hinton Agar by
using 30µg vancomycin
disc
Vancomycin resistance
can also be determined by
Vancomycin agar screen
method using 6µg/ml of
vancomycin incorporated
in Brain Heart Infusion
(BHI) agar.
40
41.
Minimum Inhibitory
Concentration (MIC) of
all the isolates were
done by Macro broth
dilution method, using
dilutions of vancomycin
ranging from 2 µg/ml to
512 µg/ml.
41
43.
*Chromogenic Methods in Diagnosis of VRE
Chromogenic
medium for the
detection of
Vancomycin
Resistant
Enterococcus (VRE)
E. faecalis and E.
faecium
* Colorex™ Prepared
Chromogenic Media by
BioMed Diagnostics
43
44.
Genotypic Detection of VRE
Rapid detection of
vancomycin
resistance by
polymerase chain
reaction (PCR). useful
in epidemiologic
studies
PCR can`t be
performed directly on
clinical specimens.
44
45.
Control and Prevention
Limiting the use of certain broad spectrum
antibiotics may also lead to a decrease in the
rates of VRE colonization and infection.
One study suggested that reduction of third-
generation cephalosporins with the
substitution of piperacillin/tazobactam could
reduce the incidence of VRE in an intensive
care unit setting
Hospital Infection Control Practices Advisory Committee (HICPAC). Recommendations for preventing the spread of
vancomycin resistance. Infect Control Hosp Epidemiol 1995; 16:105 45
46. The CDC has published recommendations for
preventing the spread of vancomycin
resistance
Prudent use of vancomycin
Education of hospital staff regarding the problem
Rapid and accurate identification of VRE in the
microbiology laboratory
Aggressive infection control measures utilizing
contact isolation and cohorting where necessary to
prevent person-to-person transmission
Hospital Infection Control Practices Advisory Committee (HICPAC). Recommendations for preventing the spread of
vancomycin resistance. Infect Control Hosp Epidemiol 1995; 16:105 46
48.
Ananthanarayan & paniker`s , Textbook of
Microbiology
Koneman`s Color Atlas and Textbook of
Diagnostic Microbilogy
Topley`s and Wilson Textbook of Microbiolgy
http://www.gbss.org.uk/filepool/BSOP58.pdf
http://www.cdc.gov/groupbstrep
References
48
Editor's Notes
This is a close-up picture of a group B Streptococcus (GBS) enriched in pigmented enrichment broth (StrepB carrot broth). The tube on the right shows a positive color change, indicating the presence of beta-hemolytic Group B strep.