This document discusses laboratory safety, with a focus on microbiology laboratories. It outlines various routes of infection in laboratories, including inoculation, ingestion, and inhalation. Microorganisms are classified into four risk groups based on their hazards. The document also discusses codes of practice, safe laboratory design features, biological safety cabinets, and other safety measures like personal protective equipment and decontamination procedures. Proper laboratory design, facilities, and biosafety management are essential for safety.
Laboratory safety rules are a major aspect of every clinical lab.
Each student in clinical laboratory must follow specific safety rules and procedures.
Laboratory Hazards, Accidents and Safety RulesTapeshwar Yadav
Injury, damage and loss by fire can be minimized when laboratory staff:
Understand how fires are caused and spread;
Reduce the risk of fire by following fire safety regulations at all times;
Know what to do if there is a fire in their laboratory;
Know how to use fire fighting equipment;
Know how to apply emergency First Aid, for burns.
Laboratory safety rules are a major aspect of every clinical lab.
Each student in clinical laboratory must follow specific safety rules and procedures.
Laboratory Hazards, Accidents and Safety RulesTapeshwar Yadav
Injury, damage and loss by fire can be minimized when laboratory staff:
Understand how fires are caused and spread;
Reduce the risk of fire by following fire safety regulations at all times;
Know what to do if there is a fire in their laboratory;
Know how to use fire fighting equipment;
Know how to apply emergency First Aid, for burns.
Managing and maintaining the integrity of laboratories is challenging, particularly if contamination is discovered. During this webinar, you will learn about several decontamination and disinfection techniques used to decontaminate spaces and equipment.
Managing and maintaining the integrity of laboratories is challenging, particularly if contamination is discovered. During this webinar, you will learn about several decontamination and disinfection techniques used to decontaminate spaces and equipment.
A 2000+ slide PowerPoint presentation from www.sciencepowerpoint.com becomes the roadmap for an amazing learning experience. Complete with homework package, built-in activities with directions, built-in quizzes, unit notes, follow along worksheets, answer keys, video links, review games, rubrics, and much more.
Also included are directions on how create a student version of the unit that is much like the teachers but missing the answer keys, quizzes, PowerPoint review games, hidden box challenges, owl, and surprises meant for the classroom. This is a great resource to distribute to your students and support professionals and will only take you a few minutes to create.
This is a great introductory unit that covers science topics associated with Lab Safety, Magnification, Base Units of the Metric System, Scientific Method, Inferences, and Observation Skills (See list below for more topics covered). This unit includes an interactive and engaging PowerPoint Presentation of 2000 slides with built in class notes (Red Slides), lab activities, project ideas, discussion questions, assessments (Quiz Wiz), and challenge questions with answers.
Text is in large print (32 font) and is placed at the top of each slide so it can seen and read from all angles of a classroom. A shade technique, as well as color coded text helps to increase student focus and allows teacher to control pace of the lessons. Also included is a 10 page assessment / bundled homework that chronologically follows the slideshow for nightly homework and end of the unit assessment, as well as a 9 page modified assessment. 14 pages of class notes with images are also included for students who require modifications, as well as answer keys to both of the assessments for support professionals, teachers, and home school parents. Several video links are provided and a slide within the slideshow cues teacher / parent when the videos are most relevant to play. Video shorts usually range from 2-7 minutes. One PowerPoint review game (125+ slides)is included. Answers to the PowerPoint review game are provided in PowerPoint form so students can self-assess. Lastly, several class games such as guess the hidden picture beneath the boxes, and the find the hidden owl somewhere within the slideshow are provided. Difficulty rating of 5 (Ten is most difficult)
Thank you for time and if you have any questions please feel free to contact me at www.sciencepowerpoint@gmail.com. Best wishes.
Teaching Duration = 4+ Weeks
Sincerely,
Ryan Murphy M.Ed
Science PowerPoints
Biosafety is the precautionary measure that reduce laboratory risk to exposure of microbe . This power point by Lamria Agnes Meilani base on WHO standard .
This presentation will describe the basic levels of biosafety in lab. the biosafety includes the safety from bio hazard material being used in lab for research.
the proper handling and transportation of the bio material or live material. the Basic levels of science laboratory with safety equipment. proper disposal and safety in case of emergency. its a very informative presentation.
deals with biosafety in medical labs. universal safety precautions included. Includes updated 8 categories and colour coding for BMW management. Being a budding microbiologist, kept it focused on microbiology lab
safety data sheet, an introduction to cell culture, safety equipment, safe laboratory practices, ascetic techniques, sterile work area, good personal hygiene, sterile reagents and media, sterile handling, planning of cell culture labs.
NVBDCP.pptx Nation vector borne disease control programSapna Thakur
NVBDCP was launched in 2003-2004 . Vector-Borne Disease: Disease that results from an infection transmitted to humans and other animals by blood-feeding arthropods, such as mosquitoes, ticks, and fleas. Examples of vector-borne diseases include Dengue fever, West Nile Virus, Lyme disease, and malaria.
These simplified slides by Dr. Sidra Arshad present an overview of the non-respiratory functions of the respiratory tract.
Learning objectives:
1. Enlist the non-respiratory functions of the respiratory tract
2. Briefly explain how these functions are carried out
3. Discuss the significance of dead space
4. Differentiate between minute ventilation and alveolar ventilation
5. Describe the cough and sneeze reflexes
Study Resources:
1. Chapter 39, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 34, Ganong’s Review of Medical Physiology, 26th edition
3. Chapter 17, Human Physiology by Lauralee Sherwood, 9th edition
4. Non-respiratory functions of the lungs https://academic.oup.com/bjaed/article/13/3/98/278874
Couples presenting to the infertility clinic- Do they really have infertility...Sujoy Dasgupta
Dr Sujoy Dasgupta presented the study on "Couples presenting to the infertility clinic- Do they really have infertility? – The unexplored stories of non-consummation" in the 13th Congress of the Asia Pacific Initiative on Reproduction (ASPIRE 2024) at Manila on 24 May, 2024.
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Flu Vaccine Alert in Bangalore Karnatakaaddon Scans
As flu season approaches, health officials in Bangalore, Karnataka, are urging residents to get their flu vaccinations. The seasonal flu, while common, can lead to severe health complications, particularly for vulnerable populations such as young children, the elderly, and those with underlying health conditions.
Dr. Vidisha Kumari, a leading epidemiologist in Bangalore, emphasizes the importance of getting vaccinated. "The flu vaccine is our best defense against the influenza virus. It not only protects individuals but also helps prevent the spread of the virus in our communities," he says.
This year, the flu season is expected to coincide with a potential increase in other respiratory illnesses. The Karnataka Health Department has launched an awareness campaign highlighting the significance of flu vaccinations. They have set up multiple vaccination centers across Bangalore, making it convenient for residents to receive their shots.
To encourage widespread vaccination, the government is also collaborating with local schools, workplaces, and community centers to facilitate vaccination drives. Special attention is being given to ensuring that the vaccine is accessible to all, including marginalized communities who may have limited access to healthcare.
Residents are reminded that the flu vaccine is safe and effective. Common side effects are mild and may include soreness at the injection site, mild fever, or muscle aches. These side effects are generally short-lived and far less severe than the flu itself.
Healthcare providers are also stressing the importance of continuing COVID-19 precautions. Wearing masks, practicing good hand hygiene, and maintaining social distancing are still crucial, especially in crowded places.
Protect yourself and your loved ones by getting vaccinated. Together, we can help keep Bangalore healthy and safe this flu season. For more information on vaccination centers and schedules, residents can visit the Karnataka Health Department’s official website or follow their social media pages.
Stay informed, stay safe, and get your flu shot today!
micro teaching on communication m.sc nursing.pdfAnurag Sharma
Microteaching is a unique model of practice teaching. It is a viable instrument for the. desired change in the teaching behavior or the behavior potential which, in specified types of real. classroom situations, tends to facilitate the achievement of specified types of objectives.
Ethanol (CH3CH2OH), or beverage alcohol, is a two-carbon alcohol
that is rapidly distributed in the body and brain. Ethanol alters many
neurochemical systems and has rewarding and addictive properties. It
is the oldest recreational drug and likely contributes to more morbidity,
mortality, and public health costs than all illicit drugs combined. The
5th edition of the Diagnostic and Statistical Manual of Mental Disorders
(DSM-5) integrates alcohol abuse and alcohol dependence into a single
disorder called alcohol use disorder (AUD), with mild, moderate,
and severe subclassifications (American Psychiatric Association, 2013).
In the DSM-5, all types of substance abuse and dependence have been
combined into a single substance use disorder (SUD) on a continuum
from mild to severe. A diagnosis of AUD requires that at least two of
the 11 DSM-5 behaviors be present within a 12-month period (mild
AUD: 2–3 criteria; moderate AUD: 4–5 criteria; severe AUD: 6–11 criteria).
The four main behavioral effects of AUD are impaired control over
drinking, negative social consequences, risky use, and altered physiological
effects (tolerance, withdrawal). This chapter presents an overview
of the prevalence and harmful consequences of AUD in the U.S.,
the systemic nature of the disease, neurocircuitry and stages of AUD,
comorbidities, fetal alcohol spectrum disorders, genetic risk factors, and
pharmacotherapies for AUD.
These lecture slides, by Dr Sidra Arshad, offer a quick overview of physiological basis of a normal electrocardiogram.
Learning objectives:
1. Define an electrocardiogram (ECG) and electrocardiography
2. Describe how dipoles generated by the heart produce the waveforms of the ECG
3. Describe the components of a normal electrocardiogram of a typical bipolar leads (limb II)
4. Differentiate between intervals and segments
5. Enlist some common indications for obtaining an ECG
Study Resources:
1. Chapter 11, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 9, Human Physiology - From Cells to Systems, Lauralee Sherwood, 9th edition
3. Chapter 29, Ganong’s Review of Medical Physiology, 26th edition
4. Electrocardiogram, StatPearls - https://www.ncbi.nlm.nih.gov/books/NBK549803/
5. ECG in Medical Practice by ABM Abdullah, 4th edition
6. ECG Basics, http://www.nataliescasebook.com/tag/e-c-g-basics
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Ozempic: Preoperative Management of Patients on GLP-1 Receptor Agonists Saeid Safari
Preoperative Management of Patients on GLP-1 Receptor Agonists like Ozempic and Semiglutide
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Pulmonary Thromboembolism - etilogy, types, medical- Surgical and nursing man...VarunMahajani
Disruption of blood supply to lung alveoli due to blockage of one or more pulmonary blood vessels is called as Pulmonary thromboembolism. In this presentation we will discuss its causes, types and its management in depth.
Recomendações da OMS sobre cuidados maternos e neonatais para uma experiência pós-natal positiva.
Em consonância com os ODS – Objetivos do Desenvolvimento Sustentável e a Estratégia Global para a Saúde das Mulheres, Crianças e Adolescentes, e aplicando uma abordagem baseada nos direitos humanos, os esforços de cuidados pós-natais devem expandir-se para além da cobertura e da simples sobrevivência, de modo a incluir cuidados de qualidade.
Estas diretrizes visam melhorar a qualidade dos cuidados pós-natais essenciais e de rotina prestados às mulheres e aos recém-nascidos, com o objetivo final de melhorar a saúde e o bem-estar materno e neonatal.
Uma “experiência pós-natal positiva” é um resultado importante para todas as mulheres que dão à luz e para os seus recém-nascidos, estabelecendo as bases para a melhoria da saúde e do bem-estar a curto e longo prazo. Uma experiência pós-natal positiva é definida como aquela em que as mulheres, pessoas que gestam, os recém-nascidos, os casais, os pais, os cuidadores e as famílias recebem informação consistente, garantia e apoio de profissionais de saúde motivados; e onde um sistema de saúde flexível e com recursos reconheça as necessidades das mulheres e dos bebês e respeite o seu contexto cultural.
Estas diretrizes consolidadas apresentam algumas recomendações novas e já bem fundamentadas sobre cuidados pós-natais de rotina para mulheres e neonatos que recebem cuidados no pós-parto em unidades de saúde ou na comunidade, independentemente dos recursos disponíveis.
É fornecido um conjunto abrangente de recomendações para cuidados durante o período puerperal, com ênfase nos cuidados essenciais que todas as mulheres e recém-nascidos devem receber, e com a devida atenção à qualidade dos cuidados; isto é, a entrega e a experiência do cuidado recebido. Estas diretrizes atualizam e ampliam as recomendações da OMS de 2014 sobre cuidados pós-natais da mãe e do recém-nascido e complementam as atuais diretrizes da OMS sobre a gestão de complicações pós-natais.
O estabelecimento da amamentação e o manejo das principais intercorrências é contemplada.
Recomendamos muito.
Vamos discutir essas recomendações no nosso curso de pós-graduação em Aleitamento no Instituto Ciclos.
Esta publicação só está disponível em inglês até o momento.
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2. Safety in Microbiology LaboratorySafety in Microbiology Laboratory
Contents
Introduction
Routes of Infection
Classification of infective
organism based on hazards
Code of Practice
Safe Laboratory Design
Biological saftey cabinets
Others Saftey
Summary
3.
4. IntroductionIntroduction
Clinical laboratory personnel including support
employees are prone to risk of
infection,chemicals,radiations etc. Such risks can be
prevented or minimized by a laboratory safety
programmes.
The attitude “What you don’t know can’t hurt you’’ is
common among laboratory employee, in the beginning
of 1980, this relax attitude toward safety among lab
personnel changed dramatically due to HIV.
5. Many laboratory workers, researchers & scientists
suffered & also lose their life such as the scientists
like Ricketts and Karls Urbani died while doing
research on finding the cause of Rocky mountain
fever and the cause of Severe acute respiratory
syndrome (SARS) respectively. They might have
breach the necessary safety guidelines while working
or doing research.
The routes by which infections are acquired in the
laboratory may be different from natural infection.
6. The varieties and concentrations of microorganisms
found in a microbiology laboratory are such that
special precautions must be taken for the safety of
the staff/student and those around him or her.
With passage of time the concepts of laboratory
safety expanded to include chemical, radioactive,
electrical & fire hazard protection.
7. Routes of infectionRoutes of infection
Inoculation:-
Literally inoculation means the introduction of material into
an ‘eye’, but it covers deliberate or accidental introduction
of infection into body by splashing or by rubbing with
contaminated fingers ,injection through skin,by needle
stick injury or bite of ectoparasite, Incision by sharp
instrument, broken glass
8. Ingestion:-
By oral route sucking or accidental swallowing of
infective materials eg. Mouth pipetting of cultures or
infected fluids, contamination of fingers during
eating , drinking or smoking in the laboratory
9. InhalationInhalation
Infection by breathing in of infected aerosol or dust.
An aerosol is a cloud of small droplets of liquid in
air. It usually contain many droplets of liquid in air;
Droplets smaller than 0.1 mm diameter dry rapidly,
become solid residues called “droplet nuclei”.
10. Classification of infective microorganisms
based on Hazards
Risk Group 1 (no or low individual and community risk)
A microorganism that is unlikely to cause human
disease.
Example: Bacillus subtilis, common moulds, Yeasts
Risk Group 2 (moderate individual risk, low community
risk)
A pathogen that can cause human disease and may
be a hazard to lab workers; effective treatment and
preventive measures are available.
Example: Staphylococcus, Streptococcus, Shigella,
Vibrio, Poliovirus, Hepatitis virus etc.
11. Risk Group 3 (high individual risk, low community risk)
A pathogen that usually causes serious human disease and
present a serious hazard to the laboratory workers
• May pose a risk of spread in the community
• Effective treatment and preventive measures are available.
Example: Bacillus anthracis, Mycobacterium tuberculosis,
Brucella, Histoplasma, Rickettsia.
• Risk Group 4 (high individual and community risk)
A pathogen that usually causes serious human disease and that can
be readily transmitted from one individual to another, directly or
indirectly.
No vaccine & chemotherapy available
– Includes all viruses,which are of high risk of Laboratory workers &
community.
– Effective treatment and preventive measures are not usually
available. Example: SARS, Ebola, Lassa, Marburg, Variola Major
12. Code of practice/safety measures/standardCode of practice/safety measures/standard
precautionprecaution
In Britain (1978) ‘Howie code’ documented ‘Code of
practice’ or in general term laboratory practice.
In 1987,the CDC published guidelines known as
“Universal precautions to reduce the risk of HBV
transmission in clinical lab& blood bank.”
In 1996 ,CDC & OSHA (US occupational safety &
health administration) jointly published ‘’Standard
precautions’’ which simply stated , blood & body
fluids from all patients be treated as infectious
materials.
13. Precautions to prevent Transmission ofPrecautions to prevent Transmission of
infection in clinical laboratoryinfection in clinical laboratory
WHO , Laboratory biosafety manual, 3WHO , Laboratory biosafety manual, 3rdrd
editionedition
20042004
Access
The international biohazard warning symbol
and sign must be displayed on the doors of
microbiology lab.
Only authorized persons should be allowed
to enter the laboratory working areas.
Laboratory doors should be kept closed.
Children and pet animal should not be
allowed to enter laboratory working areas.
No animals should be admitted other than those
Involved in the work of the laboratory.
14. Personal protection
Laboratory coveralls, gowns or uniforms
must be worn at all times for work
in the laboratory.
Appropriate gloves must be worn for all
procedures that may involve direct or
accidental contact with blood, body fluids
and other potentially infectious materials
or infected animals.
Personnel must wash their hands after
handling infectious materials and
animals and before they leave the
laboratory working areas.
15. Safety glasses, face shields or other
protective devices must be worn .
It is prohibited to wear protective
laboratory clothing outside the lab.
Open-toed footwear must not be worn
in laboratories.
Eating, drinking, smoking, applying cosmetics
and handling contact lenses is prohibited
in the laboratory working areas.
Protective laboratory clothing that has been
used in the laboratory must not be stored in
the same lockers or cupboards as street
clothing.
16. Procedures
Pipetting by mouth must be strictly forbidden.
Materials must not be placed in the mouth.
Labels must not be licked.
All technical procedures should be performed
in a way that minimizes the formation
of aerosols and droplets.
The use of hypodermic needles and
syringes should be limited.
All spills, accidents and overt or potential
exposures to infectious materials must be
reported to the laboratory supervisor.
A written record of such accidents and
incidents should be maintained.
17. Laboratory working areas
The laboratory should be kept neat, clean
and free of materials that are not pertinent
to the work.
Work surfaces must be decontaminated
after any spill of potentially dangerous
material and at the end of the working day.
All contaminated materials, specimens
and cultures must be decontaminated
before disposal or cleaning for reuse.
Packing and transportation must follow
applicable national and/or international
regulations.
18. Biosafety management
The laboratory supervisor should ensure
the development and adoption of a
biosafety management plan and a safety
or operations manual.
The laboratory supervisor should ensure
that regular training in laboratory safety
is provided.
Personnel should be advised of
special hazards,and required to read
the safety or operations manual and
follow standard practices and procedures.
19. A copy of the safety or operations
manual should be available in the
laboratory.
There should be an arthropod and
rodent control programme
Appropriate medical evaluation,
surveillance and treatment
should be provided for all
personnel in case of need,
and adequate medical records
should be maintained
20. Laboratory design and facilities
In designing a laboratory and assigning certain types
of work to it, special attention should be paid to
conditions that are known to pose safety problems.
These include:
• Formation of aerosols
• Work with large volumes and/or high concentrations of
microorganisms
• Overcrowding and too much equipment
• Infestation with rodents and arthropods
• Unauthorized entrance
• Workflow: use of specific samples and reagents.
21. Design features
Ample space must be provided for the safe conduct of laboratory
work and cleaning
Walls, ceilings and floors should be smooth, easy to clean,
impermeable to liquids and resistant to the chemicals and
disinfectants normally used in the laboratory. Floors should be
slip-resistant.
Bench tops should be impervious to water and resistant to
disinfectants, acids and alkalis etc
Open spaces between and under benches, cabinets and
equipment should be accessible for cleaning.
Storage space must be adequate to hold supplies for immediate
use.
Additional long-term storage space, conveniently located
outside the laboratory working areas, should also be provided.
22. Space and facilities should be provided for the safe
handling and storage of solvents, radioactive materials,
and compressed and liquefied gases.
Separate room storage of personal belongings and for
fooding and rest
Hand-washing basins, with running water if possible,
should be provided in each laboratory room, preferably
near the exit door.
Doors should have vision panels, appropriate fire ratings,
and preferably be selfclosing.
Safety systems should cover fire, electrical emergencies,
emergency shower and eyewash facilities.
First-aid areas or rooms suitably equipped and readily
accessible should be available
23. mechanical ventilation systems that provide an inward flow
of air without recirculation.
Good quality water is essential. There should be no
crossconnections between sources of laboratory and
drinking-water supplies.
There should be a reliable and adequate electricity supply
and emergency lighting to permit safe exit. A stand-by
generator is desirable for the support of essential
equipment, such as incubators, biological safety cabinets,
freezers, etc., and for the
ventilation of animal cages.
There should be a reliable and adequate supply of gas.
Good maintenance of the installation is mandatory.
29. Don`t forget Others Safety…………………
Fire safety
Electrical safety
Chemicals and radioactive substances safety
Engineering control & equipments eg fume hood,
closed centrifuge,Biosafety cabinets.
Safety programs also involve disaster preparedness
plans that outline steps to take in an emergency (In
areas of the country prone to natural disasters like
earthquake, hurricanes, snowstorms )
30. SummarySummary
• Proper collection, treatment and disposal of infectious waste
reduce the rate of infection, prevent aerosol formation and
protects environmental pollution.
• All infectious and non-infectious waste materials must be
collected separately and disposed properly.
• Use of red, yellow and blue color-coded bag is useful for
proper disposal of waste.
• Autoclaving, incineration and disinfection are useful means for
disposal of infectious waste.
• Now sophisticated biosafety systems & improved training
methods are necessary to cope with the increased challenges
raised by the emergence & reemergence of significant
pathogens.
• Proper use of infection control manual and formulation of
infection control committee is essential in each hospital, blood
transfusion center and laboratory institutions.