This document discusses microbial identification methods. It begins by outlining the objectives and topics to be covered, including identification of bacteria, fungi, algae, and viruses. For bacteria, it describes phenotypic methods like morphology, physiology/biochemistry, and genotypic techniques using genetic markers. Morphological identification of bacteria involves shape, staining, and colony appearance. Physiological/biochemical tests examine enzyme production and nutrient metabolism. Genotypic methods like nucleic acid sequencing and PCR are also discussed. The document continues by addressing identification of fungi, algae, and viruses through their distinguishing characteristics and laboratory techniques.
Secondary screening of industrial important microbes DhruviSuvagiya
Detection and isolation of a microorganism from a natural environment like soil containing large number of microbial population is called as screening. It is very time consuming and expensive process.
Basic Knowledge about industrial microorganism. why industry choose microorganism rather than chemical. isolation technique of microorganism. source of microorganisms. Process of using microorganism. Disadvantages of using microorganisms in industry. Process of genetic modification of microorganisms. Storage process of microorganism. preservation methods of microorganism. Reculture methods of microorganism.
Sergei Nikolaievich Winogradsky And Martinus Willem Beijerinck-Discoveries,Nitrogen Fixing Bacteria and the Discovery of Chemosynthesis, Scientific contributions
Methanogenesis or biomethanation is the formation of methane by microbes known as methanogens. Organisms capable of producing methane have been identified only from the domain Archaea, a group phylogenetically distinct from both eukaryotes and bacteria, although many live in close association with anaerobic bacteria.
Secondary screening of industrial important microbes DhruviSuvagiya
Detection and isolation of a microorganism from a natural environment like soil containing large number of microbial population is called as screening. It is very time consuming and expensive process.
Basic Knowledge about industrial microorganism. why industry choose microorganism rather than chemical. isolation technique of microorganism. source of microorganisms. Process of using microorganism. Disadvantages of using microorganisms in industry. Process of genetic modification of microorganisms. Storage process of microorganism. preservation methods of microorganism. Reculture methods of microorganism.
Sergei Nikolaievich Winogradsky And Martinus Willem Beijerinck-Discoveries,Nitrogen Fixing Bacteria and the Discovery of Chemosynthesis, Scientific contributions
Methanogenesis or biomethanation is the formation of methane by microbes known as methanogens. Organisms capable of producing methane have been identified only from the domain Archaea, a group phylogenetically distinct from both eukaryotes and bacteria, although many live in close association with anaerobic bacteria.
BIS2C. Biodiversity and the Tree of Life. 2014. L8. Intro to Microbial Divers...Jonathan Eisen
BIS2C. Biodiversity and the Tree of Life.
At UC Davis Spring 2014.
Lecture 8.
Introduction to Microbial Diversity, part 2.
Slides for Lectures by Jonathan Eisen
BIS2C. Biodiversity and the Tree of Life. 2014. L7. Intro to Microbial Divers...Jonathan Eisen
BIS2C. Biodiversity and the Tree of Life.
At UC Davis Spring 2014.
Lecture 7.
Introduction to Microbial Diversity.
Slides for Lectures by Jonathan Eisen
Advanced Onsite Wastewater Treatment Technologies and Nitrogen RemovalPete Sabo
Over 360,000 homes on Long Island have inadequate wastewater treatment technologies. Long Island
recently revamped their onsite wastewater treatment laws to mitigate this environmental degradation
from septic systems. This seminar will present information about the advanced wastewater treatment
technologies that significantly reduce the impact of nitrogen on the environment. The presentation will
focus on the role of aerobic treatment technologies and other advanced onsite wastewater treatment
technologies.
Effects of Fungicides for Non Target Fungi Alternaria cassiaeIJEAB
The fungicides are used to control of pathogenic fungi in several tilth but they can affect negatively the microorganisms diversity of soil. The aim of this research was to evaluate the toxicity and environmental risk of tebuconazoles: captan, tebuconazole and the mixture chlorothalonil + propamocarb hidrochloride for fungi Alternaria cassiae. Each fungicide were performed three experiments in completely randomized design with three repetitions and the growth was evaluated daily. Inhibition concentration (IC50;7d) of tebuconazole was 3.49 mg L-1, the captan was 47.36 mg L-1 and of mixture chlorothalonil + propamocarb hidrochloride, 64.04 mg L-1. Tebuconazole is classified as moderately toxic and sensitivity, captan, low toxicity and sensitivity and the mixture, non toxic and insensitive but only captan showed possibility of adverse effect for A. cassiae.
Bajarang enviro Engineers was established in 2006 with a aim to offer innovative ‘Environmental friendly’ & ‘Cost Effective’ Products for Wastewater Treatment, Sewage Treatment, Effluent Treatment Plants. We are manufacturers of MBBR media ,Tube settlers media,Fill pac media,Coarse bubble disc diffuser,Fine bubble tube diffusers and Fine bubble disc diffusers etc.
we have supplied media to many clients & plants are running well without any problem. Plants are running with our supplied media since more than 5 years. We are confident that our best quality media will have life of 12 to 15 years who will run the plant continuosly.
We are well equipped with sophisticated machines and technology that empowers us to provide effective Products. We assure you that our media is very durable& doesn’t require any top-up or replacement till 15 years which will runs continuosly. We guarantee our media life of more than 15 years for plant in working conditions.
Diagnostic Medical Microbiology - Traditional and Modern approachChhaya Sawant
Updated version of Diagnostic Microbiology - Traditional and Modern approach. The presentation is an overview of conventional techniques still used in many laboratories and new technologies such as Molecular- and Protein-based testing
Identification and Detection of Microorganism esraa alaa
Molecular detection of pathogens (molecular microbiology)
is a new, dynamic and progressive spinoff of classic microbiology. It plays an important role in those clinical situations when standard microbiology (relying on the successful cultivation of potential pathogens) produces suboptimal results or completely fails.
OR
Modern approach for identification and quantification of microorganisms (pathogens) in the diagnostics of infections or foodborne illness using molecular microbiology. Broadest range of available tests and tailor-made packages.
Artificial Reefs by Kuddle Life Foundation - May 2024punit537210
Situated in Pondicherry, India, Kuddle Life Foundation is a charitable, non-profit and non-governmental organization (NGO) dedicated to improving the living standards of coastal communities and simultaneously placing a strong emphasis on the protection of marine ecosystems.
One of the key areas we work in is Artificial Reefs. This presentation captures our journey so far and our learnings. We hope you get as excited about marine conservation and artificial reefs as we are.
Please visit our website: https://kuddlelife.org
Our Instagram channel:
@kuddlelifefoundation
Our Linkedin Page:
https://www.linkedin.com/company/kuddlelifefoundation/
and write to us if you have any questions:
info@kuddlelife.org
WRI’s brand new “Food Service Playbook for Promoting Sustainable Food Choices” gives food service operators the very latest strategies for creating dining environments that empower consumers to choose sustainable, plant-rich dishes. This research builds off our first guide for food service, now with industry experience and insights from nearly 350 academic trials.
Characterization and the Kinetics of drying at the drying oven and with micro...Open Access Research Paper
The objective of this work is to contribute to valorization de Nephelium lappaceum by the characterization of kinetics of drying of seeds of Nephelium lappaceum. The seeds were dehydrated until a constant mass respectively in a drying oven and a microwawe oven. The temperatures and the powers of drying are respectively: 50, 60 and 70°C and 140, 280 and 420 W. The results show that the curves of drying of seeds of Nephelium lappaceum do not present a phase of constant kinetics. The coefficients of diffusion vary between 2.09.10-8 to 2.98. 10-8m-2/s in the interval of 50°C at 70°C and between 4.83×10-07 at 9.04×10-07 m-8/s for the powers going of 140 W with 420 W the relation between Arrhenius and a value of energy of activation of 16.49 kJ. mol-1 expressed the effect of the temperature on effective diffusivity.
Willie Nelson Net Worth: A Journey Through Music, Movies, and Business Venturesgreendigital
Willie Nelson is a name that resonates within the world of music and entertainment. Known for his unique voice, and masterful guitar skills. and an extraordinary career spanning several decades. Nelson has become a legend in the country music scene. But, his influence extends far beyond the realm of music. with ventures in acting, writing, activism, and business. This comprehensive article delves into Willie Nelson net worth. exploring the various facets of his career that have contributed to his large fortune.
Follow us on: Pinterest
Introduction
Willie Nelson net worth is a testament to his enduring influence and success in many fields. Born on April 29, 1933, in Abbott, Texas. Nelson's journey from a humble beginning to becoming one of the most iconic figures in American music is nothing short of inspirational. His net worth, which estimated to be around $25 million as of 2024. reflects a career that is as diverse as it is prolific.
Early Life and Musical Beginnings
Humble Origins
Willie Hugh Nelson was born during the Great Depression. a time of significant economic hardship in the United States. Raised by his grandparents. Nelson found solace and inspiration in music from an early age. His grandmother taught him to play the guitar. setting the stage for what would become an illustrious career.
First Steps in Music
Nelson's initial foray into the music industry was fraught with challenges. He moved to Nashville, Tennessee, to pursue his dreams, but success did not come . Working as a songwriter, Nelson penned hits for other artists. which helped him gain a foothold in the competitive music scene. His songwriting skills contributed to his early earnings. laying the foundation for his net worth.
Rise to Stardom
Breakthrough Albums
The 1970s marked a turning point in Willie Nelson's career. His albums "Shotgun Willie" (1973), "Red Headed Stranger" (1975). and "Stardust" (1978) received critical acclaim and commercial success. These albums not only solidified his position in the country music genre. but also introduced his music to a broader audience. The success of these albums played a crucial role in boosting Willie Nelson net worth.
Iconic Songs
Willie Nelson net worth is also attributed to his extensive catalog of hit songs. Tracks like "Blue Eyes Crying in the Rain," "On the Road Again," and "Always on My Mind" have become timeless classics. These songs have not only earned Nelson large royalties but have also ensured his continued relevance in the music industry.
Acting and Film Career
Hollywood Ventures
In addition to his music career, Willie Nelson has also made a mark in Hollywood. His distinctive personality and on-screen presence have landed him roles in several films and television shows. Notable appearances include roles in "The Electric Horseman" (1979), "Honeysuckle Rose" (1980), and "Barbarosa" (1982). These acting gigs have added a significant amount to Willie Nelson net worth.
Television Appearances
Nelson's char
1. SB20103 MICROBE DIVERSITY
Week 4: MICROBIAL IDENTIFICATION METHODS
Jaya Seelan Sathiya Seelan PhD
Institute for Tropical Biology and Conservation, UMS
2. OBJECTIVE
• To introduce students to the basic
tools and techniques used to identify
and characterize microorganisms.
3. OUTLINE
• Identification of Bacteria
• Identification of Fungi
• Identification of Algae
• Identification of Virus
4. IDENTIFICATION OF BACTERIA
• Methods used to identify bacteria to the level
of genus and species
• Phenotypic methods
• Morphology
• Physiology or biochemistry
• Immunologic method
• Serological analysis
• Genotypic techniques
• Genetic markers (18S/16S ribosomal gene) for
identifying bacteria
12. PHYSIOLOGICAL/BIOCHEMICAL
CHARACTERISTICS
• Traditional bacterial identification
• Diagnostic tests for determining the presence of
specific enzymes and assessing nutritional and
metabolic activities
• Examples
• Fermentation of sugars
• Capacity to metabolize complex polymers
• Production of gas
• Presence of enzymes
• Sensitivity to antimicrobial drugs
14. CHEMICAL ANALYSIS
• Analyzing the types of specific
structural substances that the
microorganism contains
• Examples
• Chemical composition of
peptides in the cell wall
• Lipids in membranes
• Antibiogram
15. • Where do you find these
bacteria?
• As a microbiologist, how
do you interpret this
chart?
• Can you ID the species
level?
Critical thinking
16. GENOTYPIC METHODS
•Primary advantage over phenotypic
methods:
•Hybridization
•Nucleic Acid Sequencing and rRNA Analysis
•Polymerase Chain Reaction (PCR)-Multiplex
PCR
17. IMMUNOLOGIC METHODS
•Antibody response to antigens
•Blood testing- often easier than testing
for the microbe itself
•Serology
•Laboratory kits available for immediate
identification of a number of pathogens
18. • Characteristics of antibodies
can reveal the history of a
patient’s contact with
microorganisms or other
antigens
Serology
• Deals with in vitro diagnostic
testing of the serum
20. HOW TO IDENTIFY FUNGI?
• Morphology: under different media; growth
forms, colony appearance, growth rates, spore
shapes and sized
• Physiology: pH, Temperature and etc.
• Chemical: Secondary metabolites, toxins
• Phylogeny: Very important and up to date
(Fungal Barcode DNA, ITS)
22. IDENTIFICATION OF ALGAE
•Color
•Type of photosynthetic food reserve
•Flagella type, cell wall structure and
composition
•Morphology vs phylogeny methods
Where do you look for Algae?
25. GAME: WHAT AM I?
• In this game, each group will be given a
microbe (card) to discuss.
• Identify your microbe
• You may find all the relevant information
from the web.
• Identify their scientific name, where you
can find them, their role and etc. (Be
concise)
• Describe the identification methods.
• Be creative!
Learning objective
• Appreciate the vast
abundance and
diversity of microbes
• Able to classify microbes
based on their
characters
• Understand that most
microbes are not just
harmful
26. EXAMPLE
• Gram-negative (bacteria)
• Facultative anaerobic
• Rod shape
• Genus/species: Escherichia coli
• Found in lower intestine; in soil
• Harmless: producing vitamin K2
• Pathogenic to human: gastroenteritis, urinary tract infections, and neonatal meningitis
• E.coli #0157:H7, that produces a toxin called the Shiga toxin (classified as a bioterrorist agent)
• Have been used in vaccine development, bioremediation, production of biofuels, lighting, and production of
immobilized enzymes
Identification method: E.coli
Rapid test
-EMB Agar- glowing
colonies
Indole test (+)
• Glucose fermentation with
gas production, urea and
H2S
• Lactose
• Sucrose; mannitol
cellobiose
• Citric Acid
-
+
-
-
