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UNIT 3
Study of morphology, classification,
reproduction/replication and cultivation of
Fungi and Viruses
INTRODUCTION TO FUNGI
• It is a group of eukaryotic micro-organism
• All fungi are aerobic in nature (except some yeast which are facultative
anaerobic)
• They divide asexually, sexually or by both processes
• They may be unicellular or multicellular
• The branch of science which deals with the study of fungi is called
Mycology
CLASSIFICATION OF FUNGI
1. Depending on the cell morphology, fungi can be divided into 4 classes:
Dimorphic
fungi
Yeast like
fungi
Yeast
Molds
CLASSIFICATION OF FUNGI
Deuteromycetes
Basidiomycetes
Ascomycetes
Zygomycetes
2. Depending on their spore formation:
Depending on the cell morphology, fungi
are divided as:
1. MOULDS
• Fungi which form mycelia or hyphae are called molds/filamentous fungi
• It contains multiple identical nuclei
• They are strictly aerobic and optimum temperature for growth is 22 ºC -28 ºC
• Reproduce through small spores by sexual or asexual mode
form
+
INTRODUCTION TO VIRUS
3)
DISINFECTION
Classification and Mode of Action of
Disinfectants
SAME AS UNIT II Chemical Sterilization Techniques
(Refer Slides 68-78 from UNIT II ppt)
Factors influencing disinfection
1. Concentration of disinfectant
2. Temperature
3. Time of exposure/contact
4. pH of environment
5. Formulation of Disinfectant
6. Chemical structure of disinfectant
7. Type and no. of micro-organisms present
8. Interfering substances in the environment
Evaluation of Disinfectants
• The common methods used for the evaluation of disinfectant are as follows:
1. Tube Dilution Method
2. Agar Plate Method
3. Filter Paper & Cup-plate method
4. Phenol Coefficient Method
5. Kelsey Skye's Method
5. Kelsey Skye's Method
STERILITY TESTING OF PRODUCTS
(Solids, Liquids, Ophthalmic and other Sterile Products)
AS PER IP, BP & USP
ASSIGNMENT 1
1. WRITE THE STERILITY TESTING OF PRODUCTS AS PER BP & USP
2. WRITE A NOTE ON EVALUATION METHODS OF DISINFECTION
3. EXPLAIN THE LYTIC CYCLE OF VIRUS
4. WRITE A NOTE ON IMViC TEST
(Submission Date: 9th December)
UNIT 3 FUNGI-VIRUS-DISINFECTANTS SEMESTE
UNIT 3 FUNGI-VIRUS-DISINFECTANTS SEMESTE
UNIT 3 FUNGI-VIRUS-DISINFECTANTS SEMESTE
UNIT 3 FUNGI-VIRUS-DISINFECTANTS SEMESTE
UNIT 3 FUNGI-VIRUS-DISINFECTANTS SEMESTE
UNIT 3 FUNGI-VIRUS-DISINFECTANTS SEMESTE
UNIT 3 FUNGI-VIRUS-DISINFECTANTS SEMESTE
UNIT 3 FUNGI-VIRUS-DISINFECTANTS SEMESTE
UNIT 3 FUNGI-VIRUS-DISINFECTANTS SEMESTE
UNIT 3 FUNGI-VIRUS-DISINFECTANTS SEMESTE
UNIT 3 FUNGI-VIRUS-DISINFECTANTS SEMESTE
UNIT 3 FUNGI-VIRUS-DISINFECTANTS SEMESTE
UNIT 3 FUNGI-VIRUS-DISINFECTANTS SEMESTE
UNIT 3 FUNGI-VIRUS-DISINFECTANTS SEMESTE
UNIT 3 FUNGI-VIRUS-DISINFECTANTS SEMESTE

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UNIT 3 FUNGI-VIRUS-DISINFECTANTS SEMESTE

  • 1. UNIT 3 Study of morphology, classification, reproduction/replication and cultivation of Fungi and Viruses
  • 2. INTRODUCTION TO FUNGI • It is a group of eukaryotic micro-organism • All fungi are aerobic in nature (except some yeast which are facultative anaerobic) • They divide asexually, sexually or by both processes • They may be unicellular or multicellular • The branch of science which deals with the study of fungi is called Mycology
  • 3. CLASSIFICATION OF FUNGI 1. Depending on the cell morphology, fungi can be divided into 4 classes: Dimorphic fungi Yeast like fungi Yeast Molds
  • 5. Depending on the cell morphology, fungi are divided as:
  • 6. 1. MOULDS • Fungi which form mycelia or hyphae are called molds/filamentous fungi • It contains multiple identical nuclei • They are strictly aerobic and optimum temperature for growth is 22 ºC -28 ºC • Reproduce through small spores by sexual or asexual mode form
  • 7.
  • 8.
  • 9.
  • 10.
  • 11.
  • 12.
  • 13.
  • 14.
  • 15.
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  • 37.
  • 38. 3)
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  • 57.
  • 58.
  • 60.
  • 61.
  • 62.
  • 63.
  • 64.
  • 65.
  • 66. Classification and Mode of Action of Disinfectants SAME AS UNIT II Chemical Sterilization Techniques (Refer Slides 68-78 from UNIT II ppt)
  • 67. Factors influencing disinfection 1. Concentration of disinfectant 2. Temperature 3. Time of exposure/contact 4. pH of environment 5. Formulation of Disinfectant 6. Chemical structure of disinfectant 7. Type and no. of micro-organisms present 8. Interfering substances in the environment
  • 68.
  • 69.
  • 70.
  • 71.
  • 72.
  • 73.
  • 74.
  • 75. Evaluation of Disinfectants • The common methods used for the evaluation of disinfectant are as follows: 1. Tube Dilution Method 2. Agar Plate Method 3. Filter Paper & Cup-plate method 4. Phenol Coefficient Method 5. Kelsey Skye's Method
  • 76.
  • 77.
  • 78.
  • 79.
  • 80.
  • 81.
  • 82.
  • 83.
  • 84.
  • 85.
  • 87. STERILITY TESTING OF PRODUCTS (Solids, Liquids, Ophthalmic and other Sterile Products) AS PER IP, BP & USP
  • 88.
  • 89.
  • 90.
  • 91.
  • 92. ASSIGNMENT 1 1. WRITE THE STERILITY TESTING OF PRODUCTS AS PER BP & USP 2. WRITE A NOTE ON EVALUATION METHODS OF DISINFECTION 3. EXPLAIN THE LYTIC CYCLE OF VIRUS 4. WRITE A NOTE ON IMViC TEST (Submission Date: 9th December)