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UNIT 3
Study of morphology, classification,
reproduction/replication and cultivation of
Fungi and Viruses
INTRODUCTION TO FUNGI
• It is a group of eukaryotic micro-organism
• All fungi are aerobic in nature (except some yeast which are facultative
anaerobic)
• They divide asexually, sexually or by both processes
• They may be unicellular or multicellular
• The branch of science which deals with the study of fungi is called
Mycology
CLASSIFICATION OF FUNGI
1. Depending on the cell morphology, fungi can be divided into 4 classes:
Dimorphic
fungi
Yeast like
fungi
Yeast
Molds
CLASSIFICATION OF FUNGI
Deuteromycetes
Basidiomycetes
Ascomycetes
Zygomycetes
2. Depending on their spore formation:
Depending on the cell morphology, fungi
are divided as:
1. MOULDS
• Fungi which form mycelia or hyphae are called molds/filamentous fungi
• It contains multiple identical nuclei
• They are strictly aerobic and optimum temperature for growth is 22 ºC -28 ºC
• Reproduce through small spores by sexual or asexual mode
form
+
INTRODUCTION TO VIRUS
3)
DISINFECTION
Classification and Mode of Action of
Disinfectants
SAME AS UNIT II Chemical Sterilization Techniques
(Refer Slides 68-78 from UNIT II ppt)
Factors influencing disinfection
1. Concentration of disinfectant
2. Temperature
3. Time of exposure/contact
4. pH of environment
5. Formulation of Disinfectant
6. Chemical structure of disinfectant
7. Type and no. of micro-organisms present
8. Interfering substances in the environment
Evaluation of Disinfectants
• The common methods used for the evaluation of disinfectant are as follows:
1. Tube Dilution Method
2. Agar Plate Method
3. Filter Paper & Cup-plate method
4. Phenol Coefficient Method
5. Kelsey Skye's Method
5. Kelsey Skye's Method
STERILITY TESTING OF PRODUCTS
(Solids, Liquids, Ophthalmic and other Sterile Products)
AS PER IP, BP & USP
ASSIGNMENT 1
1. WRITE THE STERILITY TESTING OF PRODUCTS AS PER BP & USP
2. WRITE A NOTE ON EVALUATION METHODS OF DISINFECTION
3. EXPLAIN THE LYTIC CYCLE OF VIRUS
4. WRITE A NOTE ON IMViC TEST
(Submission Date: 9th December)
UNIT 3 FUNGI-VIRUS-DISINFECTANTS SEMESTE
UNIT 3 FUNGI-VIRUS-DISINFECTANTS SEMESTE
UNIT 3 FUNGI-VIRUS-DISINFECTANTS SEMESTE
UNIT 3 FUNGI-VIRUS-DISINFECTANTS SEMESTE
UNIT 3 FUNGI-VIRUS-DISINFECTANTS SEMESTE
UNIT 3 FUNGI-VIRUS-DISINFECTANTS SEMESTE
UNIT 3 FUNGI-VIRUS-DISINFECTANTS SEMESTE
UNIT 3 FUNGI-VIRUS-DISINFECTANTS SEMESTE