Recombinant DNA technology involves combining DNA from two different organisms and inserting it into a host. This is done by using restriction enzymes to cut the DNA into fragments, which are then inserted into cloning vectors like plasmids, bacteriophages, or artificial chromosomes. The recombinant DNA is then inserted into a host organism using techniques like transformation or transfection. Gel electrophoresis can be used to analyze the results and identify successful recombinant clones. While cloning has potential medical applications, reproductive cloning of humans remains unsafe and controversial.
It is the basics of vector cloning which necessary for every and each student who is intrested in biotechnology. It is only starting, if you want to more than this then please comment on it.
To modifying the structure of a specific gene.
Gene targeting vector introduced into the cell.
Vector modifies the normal chromosomal gene through homologous recombination.
Useful in treating some human genetic disorders – Hemophilia, Duchenne Muscular Dystrophy.
Treating human diseases by genetic approaches – Gene Therapy.
Gene Therapy – Replacing the defective gene by normal copy of the gene.
Expressed sequence tag/EST is a short partial sequence, typically 200-400 bp long, of a complimentary DNA/Cdna.
EST is a short sub-sequence of a cDNA sequence.
Used to identify gene transcripts, and are instrumental in gene discovery and in gene-sequence determination.
Approximately 74.2 million ESTs are available in public databases.
EST results from one-short sequencing of a cloned cDNA.
Low-quality fragments.
Length is approximately 500 to 800 nucleotides.
DNA cloning is the process of making multiple, identical copies of a particular piece of DNA. In a typical DNA cloning procedure, the gene or other DNA fragment of interest (perhaps a gene for a medically important human protein) is first inserted into a circular piece of DNA called a plasmid.- [https://www.khanacademy.org/science/...dna.../dna-cloning.../a/overview-dna-cloning]
Objectives:
After the end of the presentation we’ll know -
What is cloning vector?
Why cloning vector?
History
Features of a cloning vector
Types of cloning vector
Plasmid
Bacteriophage
Cosmid
Bacterial Artificial Chromosome (BAC)
Yeast Artificial Chromosome (BAC)
Human Artificial Chromosome (HAC)
Retroviral Vectors
What determines choice of vector?
Vector in molecular gene cloning
Cloning vector - The molecular analysis of DNA has been made possible by the cloning of DNA. The two molecules that are required for cloning are the DNA to be cloned and a cloning vector.
A cloning vector is a small piece of DNA taken from a virus, a plasmid or the cell of a higher organism, that can be stably maintained in an organism and into which a foreign DNA fragment can be inserted for cloning purposes.
Most vectors are genetically engineered.
The cloning vector is chosen according to the size and type of DNA to be cloned.
The vector therefore contains features that allow for the convenient insertion or removal of DNA fragment in or out of the vector, for example by treating the vector and the foreign DNA with a restriction enzyme and then ligating the fragments together.
After a DNA fragment has been cloned into a cloning vector, it may be further subcloned into another vector designed for more specific use.
BAC & YAC are artificially prepared chromosomes to clone DNA sequences.yeast artificial chromosome is capable of carrying upto 1000 kbp of inserted DNA sequence
It is the basics of vector cloning which necessary for every and each student who is intrested in biotechnology. It is only starting, if you want to more than this then please comment on it.
To modifying the structure of a specific gene.
Gene targeting vector introduced into the cell.
Vector modifies the normal chromosomal gene through homologous recombination.
Useful in treating some human genetic disorders – Hemophilia, Duchenne Muscular Dystrophy.
Treating human diseases by genetic approaches – Gene Therapy.
Gene Therapy – Replacing the defective gene by normal copy of the gene.
Expressed sequence tag/EST is a short partial sequence, typically 200-400 bp long, of a complimentary DNA/Cdna.
EST is a short sub-sequence of a cDNA sequence.
Used to identify gene transcripts, and are instrumental in gene discovery and in gene-sequence determination.
Approximately 74.2 million ESTs are available in public databases.
EST results from one-short sequencing of a cloned cDNA.
Low-quality fragments.
Length is approximately 500 to 800 nucleotides.
DNA cloning is the process of making multiple, identical copies of a particular piece of DNA. In a typical DNA cloning procedure, the gene or other DNA fragment of interest (perhaps a gene for a medically important human protein) is first inserted into a circular piece of DNA called a plasmid.- [https://www.khanacademy.org/science/...dna.../dna-cloning.../a/overview-dna-cloning]
Objectives:
After the end of the presentation we’ll know -
What is cloning vector?
Why cloning vector?
History
Features of a cloning vector
Types of cloning vector
Plasmid
Bacteriophage
Cosmid
Bacterial Artificial Chromosome (BAC)
Yeast Artificial Chromosome (BAC)
Human Artificial Chromosome (HAC)
Retroviral Vectors
What determines choice of vector?
Vector in molecular gene cloning
Cloning vector - The molecular analysis of DNA has been made possible by the cloning of DNA. The two molecules that are required for cloning are the DNA to be cloned and a cloning vector.
A cloning vector is a small piece of DNA taken from a virus, a plasmid or the cell of a higher organism, that can be stably maintained in an organism and into which a foreign DNA fragment can be inserted for cloning purposes.
Most vectors are genetically engineered.
The cloning vector is chosen according to the size and type of DNA to be cloned.
The vector therefore contains features that allow for the convenient insertion or removal of DNA fragment in or out of the vector, for example by treating the vector and the foreign DNA with a restriction enzyme and then ligating the fragments together.
After a DNA fragment has been cloned into a cloning vector, it may be further subcloned into another vector designed for more specific use.
BAC & YAC are artificially prepared chromosomes to clone DNA sequences.yeast artificial chromosome is capable of carrying upto 1000 kbp of inserted DNA sequence
Cloning is a technique scientists use to make exact genetic copies of living things. Genes, cells, tissues, and even whole animals can all be cloned. Some clones already exist in nature. Single-celled organisms like bacteria make exact copies of themselves each time they reproduce.
Recombinant DNA (rDNA) molecules are DNA molecules formed by laboratory methods of genetic recombination (such as molecular cloning) to bring together genetic material from multiple sources, creating sequences that would not otherwise be found in the genome.
After the end of the presentation we’ll know -
What is cloning vector?
Why cloning vector?
History
Features of a cloning vector
Types of cloning vector
Plasmid
Bacteriophage
Cosmid
Bacterial Artificial Chromosome (BAC)
Yeast Artificial Chromosome (BAC)
Human Artificial Chromosome (HAC)
Retroviral Vectors
What determines choice of vector?
Vector in molecular gene cloning
In biology, cloning is the process of producing similar populations of genetically identical individuals that occurs in nature when organisms such as bacteria, insects or plants reproduce asexually. Cloning in biotechnology refers to processes used to create copies of DNA fragments (molecular cloning), cells (cell cloning), or organisms. The term also refers to the production of multiple copies of a product such as digital media or software.
Probiotics are live bacteria or yeasts that are good for the digestive system.
Prebiotics as non-digestible ingredients in the food that can stimulate the activity of desirable microbiota
Carbon and Energy Sources for Bacterial Growth, Structure of spore, The factors that plays major role for the resistance of Bacterial Spore, Sporulation, Germination
ARTIFICIAL INTELLIGENCE IN HEALTHCARE.pdfAnujkumaranit
Artificial intelligence (AI) refers to the simulation of human intelligence processes by machines, especially computer systems. It encompasses tasks such as learning, reasoning, problem-solving, perception, and language understanding. AI technologies are revolutionizing various fields, from healthcare to finance, by enabling machines to perform tasks that typically require human intelligence.
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Ethanol (CH3CH2OH), or beverage alcohol, is a two-carbon alcohol
that is rapidly distributed in the body and brain. Ethanol alters many
neurochemical systems and has rewarding and addictive properties. It
is the oldest recreational drug and likely contributes to more morbidity,
mortality, and public health costs than all illicit drugs combined. The
5th edition of the Diagnostic and Statistical Manual of Mental Disorders
(DSM-5) integrates alcohol abuse and alcohol dependence into a single
disorder called alcohol use disorder (AUD), with mild, moderate,
and severe subclassifications (American Psychiatric Association, 2013).
In the DSM-5, all types of substance abuse and dependence have been
combined into a single substance use disorder (SUD) on a continuum
from mild to severe. A diagnosis of AUD requires that at least two of
the 11 DSM-5 behaviors be present within a 12-month period (mild
AUD: 2–3 criteria; moderate AUD: 4–5 criteria; severe AUD: 6–11 criteria).
The four main behavioral effects of AUD are impaired control over
drinking, negative social consequences, risky use, and altered physiological
effects (tolerance, withdrawal). This chapter presents an overview
of the prevalence and harmful consequences of AUD in the U.S.,
the systemic nature of the disease, neurocircuitry and stages of AUD,
comorbidities, fetal alcohol spectrum disorders, genetic risk factors, and
pharmacotherapies for AUD.
New Directions in Targeted Therapeutic Approaches for Older Adults With Mantl...i3 Health
i3 Health is pleased to make the speaker slides from this activity available for use as a non-accredited self-study or teaching resource.
This slide deck presented by Dr. Kami Maddocks, Professor-Clinical in the Division of Hematology and
Associate Division Director for Ambulatory Operations
The Ohio State University Comprehensive Cancer Center, will provide insight into new directions in targeted therapeutic approaches for older adults with mantle cell lymphoma.
STATEMENT OF NEED
Mantle cell lymphoma (MCL) is a rare, aggressive B-cell non-Hodgkin lymphoma (NHL) accounting for 5% to 7% of all lymphomas. Its prognosis ranges from indolent disease that does not require treatment for years to very aggressive disease, which is associated with poor survival (Silkenstedt et al, 2021). Typically, MCL is diagnosed at advanced stage and in older patients who cannot tolerate intensive therapy (NCCN, 2022). Although recent advances have slightly increased remission rates, recurrence and relapse remain very common, leading to a median overall survival between 3 and 6 years (LLS, 2021). Though there are several effective options, progress is still needed towards establishing an accepted frontline approach for MCL (Castellino et al, 2022). Treatment selection and management of MCL are complicated by the heterogeneity of prognosis, advanced age and comorbidities of patients, and lack of an established standard approach for treatment, making it vital that clinicians be familiar with the latest research and advances in this area. In this activity chaired by Michael Wang, MD, Professor in the Department of Lymphoma & Myeloma at MD Anderson Cancer Center, expert faculty will discuss prognostic factors informing treatment, the promising results of recent trials in new therapeutic approaches, and the implications of treatment resistance in therapeutic selection for MCL.
Target Audience
Hematology/oncology fellows, attending faculty, and other health care professionals involved in the treatment of patients with mantle cell lymphoma (MCL).
Learning Objectives
1.) Identify clinical and biological prognostic factors that can guide treatment decision making for older adults with MCL
2.) Evaluate emerging data on targeted therapeutic approaches for treatment-naive and relapsed/refractory MCL and their applicability to older adults
3.) Assess mechanisms of resistance to targeted therapies for MCL and their implications for treatment selection
These simplified slides by Dr. Sidra Arshad present an overview of the non-respiratory functions of the respiratory tract.
Learning objectives:
1. Enlist the non-respiratory functions of the respiratory tract
2. Briefly explain how these functions are carried out
3. Discuss the significance of dead space
4. Differentiate between minute ventilation and alveolar ventilation
5. Describe the cough and sneeze reflexes
Study Resources:
1. Chapter 39, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 34, Ganong’s Review of Medical Physiology, 26th edition
3. Chapter 17, Human Physiology by Lauralee Sherwood, 9th edition
4. Non-respiratory functions of the lungs https://academic.oup.com/bjaed/article/13/3/98/278874
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Ve...kevinkariuki227
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Verified Chapters 1 - 19, Complete Newest Version.pdf
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Verified Chapters 1 - 19, Complete Newest Version.pdf
Report Back from SGO 2024: What’s the Latest in Cervical Cancer?bkling
Are you curious about what’s new in cervical cancer research or unsure what the findings mean? Join Dr. Emily Ko, a gynecologic oncologist at Penn Medicine, to learn about the latest updates from the Society of Gynecologic Oncology (SGO) 2024 Annual Meeting on Women’s Cancer. Dr. Ko will discuss what the research presented at the conference means for you and answer your questions about the new developments.
These lecture slides, by Dr Sidra Arshad, offer a quick overview of physiological basis of a normal electrocardiogram.
Learning objectives:
1. Define an electrocardiogram (ECG) and electrocardiography
2. Describe how dipoles generated by the heart produce the waveforms of the ECG
3. Describe the components of a normal electrocardiogram of a typical bipolar leads (limb II)
4. Differentiate between intervals and segments
5. Enlist some common indications for obtaining an ECG
Study Resources:
1. Chapter 11, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 9, Human Physiology - From Cells to Systems, Lauralee Sherwood, 9th edition
3. Chapter 29, Ganong’s Review of Medical Physiology, 26th edition
4. Electrocardiogram, StatPearls - https://www.ncbi.nlm.nih.gov/books/NBK549803/
5. ECG in Medical Practice by ABM Abdullah, 4th edition
6. ECG Basics, http://www.nataliescasebook.com/tag/e-c-g-basics
Flu Vaccine Alert in Bangalore Karnatakaaddon Scans
As flu season approaches, health officials in Bangalore, Karnataka, are urging residents to get their flu vaccinations. The seasonal flu, while common, can lead to severe health complications, particularly for vulnerable populations such as young children, the elderly, and those with underlying health conditions.
Dr. Vidisha Kumari, a leading epidemiologist in Bangalore, emphasizes the importance of getting vaccinated. "The flu vaccine is our best defense against the influenza virus. It not only protects individuals but also helps prevent the spread of the virus in our communities," he says.
This year, the flu season is expected to coincide with a potential increase in other respiratory illnesses. The Karnataka Health Department has launched an awareness campaign highlighting the significance of flu vaccinations. They have set up multiple vaccination centers across Bangalore, making it convenient for residents to receive their shots.
To encourage widespread vaccination, the government is also collaborating with local schools, workplaces, and community centers to facilitate vaccination drives. Special attention is being given to ensuring that the vaccine is accessible to all, including marginalized communities who may have limited access to healthcare.
Residents are reminded that the flu vaccine is safe and effective. Common side effects are mild and may include soreness at the injection site, mild fever, or muscle aches. These side effects are generally short-lived and far less severe than the flu itself.
Healthcare providers are also stressing the importance of continuing COVID-19 precautions. Wearing masks, practicing good hand hygiene, and maintaining social distancing are still crucial, especially in crowded places.
Protect yourself and your loved ones by getting vaccinated. Together, we can help keep Bangalore healthy and safe this flu season. For more information on vaccination centers and schedules, residents can visit the Karnataka Health Department’s official website or follow their social media pages.
Stay informed, stay safe, and get your flu shot today!
The prostate is an exocrine gland of the male mammalian reproductive system
It is a walnut-sized gland that forms part of the male reproductive system and is located in front of the rectum and just below the urinary bladder
Function is to store and secrete a clear, slightly alkaline fluid that constitutes 10-30% of the volume of the seminal fluid that along with the spermatozoa, constitutes semen
A healthy human prostate measures (4cm-vertical, by 3cm-horizontal, 2cm ant-post ).
It surrounds the urethra just below the urinary bladder. It has anterior, median, posterior and two lateral lobes
It’s work is regulated by androgens which are responsible for male sex characteristics
Generalised disease of the prostate due to hormonal derangement which leads to non malignant enlargement of the gland (increase in the number of epithelial cells and stromal tissue)to cause compression of the urethra leading to symptoms (LUTS
2. What is recombinant DNA?
● Recombinant DNA is the formation of a novel
DNA sequence by the formation of two DNA
strands.
● These are taken from two different organisms.
● These recombinant DNA molecules can be
made with recombinant DNA technology.
3. What is recombinant DNA technology?
The procedure is to cut the DNA of the donor
organism into pieces with restriction
enzymes, and insert one of these fragments into
the DNA of the host.
5. The History of Recombinant DNA Technology
• 1970 Hamilton Smith, at Johns
Hopkins Medical School, isolates
the first restriction enzyme, an
enzyme that cuts DNA at a very
specific nucleotide sequence.
Over the next few years, several
more restriction enzymes will be
isolated.
• 1972 Stanley Cohen and Herbert
Boyer combine their efforts to
create recombinant DNA. This
technology will be the beginning
of the biotechnology industry.
6. The History of Recombinant DNA Technology
• 1976 Herbert Boyer
cofounds Genentech, the
first firm founded in the
United States to apply
recombinant DNA
technology.
• 1978 Somatostatin, which
regulates human growth
hormones, is the first
human protein made
using recombinant
technology.
7. What Required for Recombinant DNA Technology
First ; Cloning Vectors
A cloning vector is
a small piece of
DNA into which a
foreign DNA fragment
can be inserted.
8. What are the kinds of Cloning Vectors
1- Plasmid Cloning Vectors
2- Bacteriophage Vectors
3- Cosmids Vectors
4- (BACs)
5- YACs
9. 1- Plasmid Cloning Vectors
• A plasmid is a DNA molecule
that is separate from the
chromosomal DNA.
• It can replicate
independently of the
chromosomal DNA.
• It is double-stranded and, in
many cases, circular.
• Plasmid sizes vary from 1 to
over 1,000 kilobase pairs
(kbp), but it can only contain Illustration of a bacterium with plasmid
inserts of about 1–10 kbp. enclosed showing chromosomal DNA and
plasmids
10. 1- Plasmid Cloning Vectors
• Origin of replication (ORI).
• Plasmid is used to multiply (make many copies of) or express
particular genes.
• Plasmid containing genes that make cells resistant to particular
antibiotics, Selectable marker(s)
• Plasmid containing a multiple cloning site (MCS, or polylinker).
11. 1- Plasmid Cloning Vectors
How are the plasmids inserted into bacteria?
There are two types of plasmid integration into a host bacteria: Non-integrating plasmids replicate as
with the top instance; whereas episomes, the lower example, integrate into the host chromosome
12. 1- Plasmid Cloning Vectors
Cloning DNA into a Plasmid to Produce Recombinant DNA
13. 2- Bacteriophage Vectors
● Bacteriophage is any one
of a number of viruses
that infect bacteria.
● Commonly based upon l
phage. Lambda phage is a
virus particle consisting of
a head, containing
double-stranded linear
DNA as its genetic
material, and a tail that
can have tail fibers.
The structure of a typical
tailed bacteriophage
14. 2- Bacteriophage Vectors
● Most internal genes deleted.
● Insert DNA into middle region (up to10-15 kb)
● The phage genes expressed in lysogenic cycle code for proteins
that repress expression of other phage genes.
17. 3- Cosmids Vectors
• Cosmid type of hybrid plasmid.
• Plasmid with λ phage packaging sequence (cos)
• Cosmids are able to contain 37 to 52 kb of DNA.
• Packaged into λ particles and injected into host cells.
• Circularizes in cell and continues as a large plasmid
19. 3- (BACs) Vectors
• Bacterial artificial
chromosome.
• Can clone up to 200 kb
DNA fragments.
• Based upon F plasmid.
• A similar cloning
vector, called a PAC has
also been produced from
the bacterial P1-plasmid.
• Origin, selectable
marker, promoters to
expressed cloned genes
20. 3- (BACs) Vectors
How the
BAC
inserted
into bacteria
to Produce
Recombinant
DNA?
21. 4 - (YACs) Vectors
• Yeast artificial
chromosomes
• Have
centromere, telome
res and an origin of
replication, plus
selectable markers
• Cloned segments of
250 kb
22. 4 - (YACs) Vectors
How the
YAC
inserted
into yeast to
Produce
Recombinant
DNA?
23. Summary of vectors and what they can carry.
The size of DNA that vector
Vector
can carry
0 – 10 kb Standard plasmid
0 – 23 Kb Lambda Bacteriophage
30 – 44 Kb Cosmid
70 – 100 Kb Bacteriophage P1
130 – 150 Kb P1 Artificial chromosome PAC
Maximum 300 Kb Bacterial Artificial Chromosome BAC
0.2 – 2 Mb Yeast Artificial Chromosome YAC
kb (= kbp) = kilo base pairs = 1,000 bp
Mb = mega base pairs = 1,000,000 bp
24. 5 - Expression Vectors
• Also known as an
expression
construct
• It include
regulatable high
level expression
promoter
– T7 phage
promoter
– lac operator
– lac repressor
gene
26. What Required for Recombinant DNA Technology
Second; Enzymes
A- Restriction enzyme
• OR restriction endonuclease) is an enzyme that cuts double-
stranded or single stranded DNA at specific recognition
nucleotide sequences known as restriction sites.
27. Summary of some kinds of Restriction
enzymes and their cut sites.
28. Second; Enzymes
B - A DNA ligase enzyme
• Catalyse the joining or recombining of DNA fragments
(ligation).
DNA ligase Ligation by DNA ligase enzyme
29. What the steps for recombinant DNA technology?
• Protocol;
– Isolate target DNA
– Cut with RE
– Ligate to vector
– Transform to host
cells
– Plate on antibiotic-
containing medium
– Identify recombinant
plasmids
– Identify/characterize
specific clones
31. Examining the results of a
restriction digest ( after recombinant )
After treatment with a
restriction
endonuclease,
the resulting DNA
fragments can be
examined
by agarose gel
electrophoresis to
determine
their sizes and to make An agarose gel plate. The hybridized DNA
shore if recombinant strands show a pink light because of the
DNA happened or not. binding of the fluorescent labeled probe.
32. What is Gel Electrophoresis
• A technique used to separate DNA fragments by
size
• The gel (agarose or polyacrylamide) is subjected to
an electrical field
• The DNA, which is negatively-charged, migrates
towards the positive pole.
• The larger the DNA fragment, the slower it will
move through the gel matrix.
• DNA is visualized using fluorescent dyes.
35. Reproductive Cloning
* Reproductive cloning is
performed with the
express intent of creating
another organism.
* This organism is the exact
duplicate of one that
already exists or has existed
in the past.
* Cloning of
plants, animals, and
humans falls into the class
of reproductive cloning
36. How is Reproductive Cloning Performed?
* It is performed using a technique called Somatic Cell Nuclear Transfer (SCNT).
* The genetic material from a donor egg is removed, so that you are left with an
empty egg.
* Then, a cell is taken from the organism to be cloned and its nucleus is removed.
* This nucleus is then transferred into the empty donor egg.
37. What Is Reproductive Cloning Used For?
Reproductive cloning has only
been used for research
purposes.
Reproductive cloning could be
used effectively for
repopulating endangered
species or to help make
breeding of specific animals
easier.
Reproductive cloning uses could
also include the production of
organisms with specific
characteristics, such as drug-
producing animals or
genetically "unique" animals.
38. Dolly was created in a process called
“Somatic Cell Nuclear Transfer”
Professor Ian Wilmut is now a Sir for his work creating the world’s first cloned
mammal in 1996 - 2003
39. Therapeutic Cloning
• It is performed, not to
produce another
organism, but to harvest
embryonic stem cells for
use in medical treatments.
• Embryonic stem cells are
those cells found inside of
developing embryos.
• They can be used to
produce a number of
different cells including
tissue, muscle, and organ
cells.
40. How is Therapeutic Cloning Performed?
* A cell is removed from the patient requiring medical treatment.
* The nucleus of this cell is removed and inserted into an empty donor egg.
* Division is encouraged through the use of special chemicals or an electric
current.
* The resulting embryonic stem cells are then removed from this embryo and
used to treat the patient.
41. What is Therapeutic Cloning Used For?
• Therapeutic cloning is intended for medical use.
• The embryonic stem cells that this type of cloning produces can be
used to create skin for burn victims, organs for transplant patients, or
cells for those with spinal cord injuries.
42. What is Therapeutic Cloning Used For?
• And because the cells come from the patient herself, there are
no issues of cell rejection.
• Therapeutic cloning may also help those suffering from heart
disease, Alzheimer's Disease, or Parkinson's Disease.
43. Can organs be cloned for use in transplants?
Scientists think that therapeutic
cloning can be used to make
tissues and organs for transplants.
To do this, DNA would be taken
from the person who needs the
transplant and put in a egg. After
the egg with the patient's DNA
starts to divide, embryonic stem
cells that can be transformed into
any type of tissue would be
harvested..
Stem cells would generate an
organ or tissue that is a genetic
match to the recipient, as a result
organ donation would reduce
significantly.
44. Risks of Cloning
More than 90% of cloning attempts fail to produce viable
offspring
Cloned animals tend to have higher rates of infection tumor
growth, and other disorders.
Clones have been known to die mysteriously.
45. Because of the lack of
understanding about
reproductive cloning
scientists believe it is
unethical to attempt
to clone humans.
About 30% of clones
born alive are
affected with “large
offspring syndrome”
46. • Several cloned
animals have died
prematurely from
infections or other
complications, the
same problems would
be expected in human
cloning
• The attempt to clone
humans at this time is
considered
potentially dangerous,
and ethically
irresponsible.