This document discusses the normal microbial flora of the skin, intestines, and vagina. It describes the most common bacteria that normally inhabit these areas, including Staphylococcus epidermidis and Streptococcus on the skin. In the intestines, it notes the high numbers of anaerobic bacteria and how the flora changes along the GI tract. It also explains how disruption of the normal flora through factors like antibiotics or medical procedures can allow pathogenic bacteria to colonize.
Mechanism of pathogenicity-Exotoxin and endotoxinaiswarya thomas
Brief description on mechanisms of pathogenicity, actions of toxins produced by various bacteria and notable endotoxins and exotoxins. Mechanism of action of some of the commonest endotoxins and exotoxins are explained.
Cholera is a serious bacterial disease that usually
causes severe diarrhea and dehydration. The disease is typically spread through contaminated water.
Modern sewage and water treatment have effectively eliminated cholera in most countries. It’s still a problem in countries like Asia, America and Africa. Mostly in India.
Countries affected by war, poverty, and natural disasters have the greatest risk for a cholera outbreak.
Taxonomy:
class : Gamma Proteobacteria
Order: Vibrionales
Family: Vibrionaceae
Genus: Vibrio
Species: v.cholerae, v.parahaemolyticus,
v. vulnificus, v. alginolyticus
MORPHOLOGY:
Gram negative, actively motile, short, rigid curved bacilli
Resembling letter “V”
about 34 genus
most common in water
1.5µ X 0.2 -0.4 µ in size
polar flagellum , strongly aerobic
Smear – fish in stream appearance
PATHOGENESIS:
Source: Ingestion of contaminated water, food,
fruits and vegetables etc.,
Incubation periods: 1-5 days
Symptoms: Watery diarrhoea, vomiting, thirst, dehydration, muscle cramps
Complications: muscular pain, renal failure, pulmonary edema, cardiac arrhythrnias
DIAGNOSIS:
Specimen: stool sample, water sample(envt)
Microscopy: a) Hanging drop : +ve
b) Gram stain :-ve
Culture: Mac conkey Agar :colourless to light pink
TCBS : yellow colonies
Serology: serological tests are no diagnostic value
TREATMENT:
Adequate replacement of fluids and electrolytes.
Oral tetracycline reduces the period of vibrio excreation.
PREVENTION:
Drink and use bottled water
Frequent washing
Sanitary environment
Defecate in water
Cook food thoroughly
The genus Shigella exclusively infects human intestine.
Shigella dysenteriae is the causative agent of bacillary dysentery or shigellosis in humans.
It is a diarrheal illness which is characterized by frequent passage of blood stained mucopurulent stools.
The four important species of the genus Shigella are:
Shigella dysenteriae
Shigella flexneri
Shigella sonnei
Shigella boydii.
Mechanism of pathogenicity-Exotoxin and endotoxinaiswarya thomas
Brief description on mechanisms of pathogenicity, actions of toxins produced by various bacteria and notable endotoxins and exotoxins. Mechanism of action of some of the commonest endotoxins and exotoxins are explained.
Cholera is a serious bacterial disease that usually
causes severe diarrhea and dehydration. The disease is typically spread through contaminated water.
Modern sewage and water treatment have effectively eliminated cholera in most countries. It’s still a problem in countries like Asia, America and Africa. Mostly in India.
Countries affected by war, poverty, and natural disasters have the greatest risk for a cholera outbreak.
Taxonomy:
class : Gamma Proteobacteria
Order: Vibrionales
Family: Vibrionaceae
Genus: Vibrio
Species: v.cholerae, v.parahaemolyticus,
v. vulnificus, v. alginolyticus
MORPHOLOGY:
Gram negative, actively motile, short, rigid curved bacilli
Resembling letter “V”
about 34 genus
most common in water
1.5µ X 0.2 -0.4 µ in size
polar flagellum , strongly aerobic
Smear – fish in stream appearance
PATHOGENESIS:
Source: Ingestion of contaminated water, food,
fruits and vegetables etc.,
Incubation periods: 1-5 days
Symptoms: Watery diarrhoea, vomiting, thirst, dehydration, muscle cramps
Complications: muscular pain, renal failure, pulmonary edema, cardiac arrhythrnias
DIAGNOSIS:
Specimen: stool sample, water sample(envt)
Microscopy: a) Hanging drop : +ve
b) Gram stain :-ve
Culture: Mac conkey Agar :colourless to light pink
TCBS : yellow colonies
Serology: serological tests are no diagnostic value
TREATMENT:
Adequate replacement of fluids and electrolytes.
Oral tetracycline reduces the period of vibrio excreation.
PREVENTION:
Drink and use bottled water
Frequent washing
Sanitary environment
Defecate in water
Cook food thoroughly
The genus Shigella exclusively infects human intestine.
Shigella dysenteriae is the causative agent of bacillary dysentery or shigellosis in humans.
It is a diarrheal illness which is characterized by frequent passage of blood stained mucopurulent stools.
The four important species of the genus Shigella are:
Shigella dysenteriae
Shigella flexneri
Shigella sonnei
Shigella boydii.
Human Microbiome is the current project in Research field. The importance of Microorganisms in the human body, the importance and novel roe of the microorganisms on a human body is very effective and helpful. Fecal Transplantation is a unique and helpful technique to cure a dreadful disease naturally by means of microorganisms or introducing the normal flora in to the body again.
A review report on detailed study of research endeavours, undertaken on Human Microbiome, its composition, its implications, applications, disease and other role.
You will learn in this presentation the variety of stains of microorganisms that you can find in the different parts of the human body, including their abundance and implications.
Types of Normal flora
Association between human and normal flora .
Characteristics of normal flora
Tissue specificity
Specific aadherence
Biofilm formation
Normal flora of skin
Normal flora of oral cavity
Normal flora of conjunctiva
Normal flora of respiratory tract
Normal flora of gastro intestinal tract
Normal flora of urogenital tract
Beneficial effect of normal flora
Harmful effect of normal flora
Disease caused by normal flora
Normal flora is the term used to describe the various bacteria and fungi that are permanent residents of certain
body sites, especially the skin, oropharynx, colon, and
vagina (Tables 6–1 and 6–2). Viruses and parasites (protozoa
and helminths), which are the other major groups of
microorganisms, are usually not considered members of
the normal flora, although they can be present in asymptomatic individuals. The normal flora organisms are often
referred to as commensals. Commensals are organisms
that derive benefit from another host but do not damage
that host. The term human microbiome is often used to
describe the normal flora.
The members of the normal flora play a role both in the
maintenance of health and in the causation of disease in
three significant ways:
(1) They can cause disease, especially in immunocompromised and debilitated individuals. Although these
organisms are nonpathogens in their usual anatomic location, they can be pathogens in other parts of the body.
(2) They constitute a protective host defense mechanism.
The nonpathogenic resident bacteria occupy attachment
sites on the skin and mucosa that can interfere with colonization by pathogenic bacteria. The ability of members of the
normal flora to limit the growth of pathogens is called colonization resistance. If the normal flora is suppressed, pathogens may grow and cause disease. For example, antibiotics
can reduce the normal colonic flora that allows Clostridium
difficile, which is resistant to the antibiotics, to overgrow and
cause pseudomembranous colitis.
(3) They may serve a nutritional function. The intestinal bacteria produce several B vitamins and vitamin K.
Poorly nourished people who are treated with oral antibiotics can have vitamin deficiencies as a result of the reduction in the normal flora. However, since germ-free animals
are well-nourished, the normal flora is not essential for
proper nutrition.
THE HUMAN MICROBIOME
The human microbiome is the term used to describe the
thousands of microbes (“microbiota”) located on the skin,
on mucosal surfaces, and within the lumen of the
TABLE 6–2 Medically Important Members of the Normal Flora
Location Important Organisms1 Less Important Organisms2
Skin Staphylococcus epidermidis Staphylococcus aureus, Corynebacterium (diphtheroids), various streptococci,
Pseudomonas aeruginosa, anaerobes (e.g., Propionibacterium), yeasts (e.g.,
Candida albicans)
Nose S. aureus3 S. epidermidis, Corynebacterium (diphtheroids), various streptococci
Mouth Viridans streptococci Various streptococci, Eikenella corrodens
Dental plaque Streptococcus mutans Prevotella intermedia, Porphyromonas gingivalis
Gingival crevices Various anaerobes (e.g.,
Bacteroides, Fusobacterium,
streptococci, Actinomyces)
Throat Viridans streptococci Various streptococci (including Streptococcus pyogenes and Streptococcus
pneumoniae), Neisseria species, Haemophilus influenzae, S. epidermidis
Colon Bacteroides fragilis, Escherichia
The human microbiome comprises of collective genome of all the microbes residing at various sites in the human body and consisting of communities of a variety of microorganisms, including Eukaryotes, Archaea, Bacteria and the virus that reside in the different body habitat including the skin, the oral cavity, respiratory tract, gastrointestinal tract, urinary tract, reproductive tract etc. We acquire these bacteria during birth and the first year of life, and they live with us throughout our lives.
The human microbiota helps us to keep healthy, but sometimes these bacteria can also be harmful. We need to take good care of our microbiota to avoid the development of some diseases, such as obesity and asthma. We should eat healthy foods that contribute to the development of a healthy microbiota. The study of the human microbiome is important, and it gives an in-depth understanding of the interplay between humans and its indigenous microbiota. This gives valuable insight into further research studies in optimizing these organisms to combating life-threatening diseases.
New Directions in Targeted Therapeutic Approaches for Older Adults With Mantl...i3 Health
i3 Health is pleased to make the speaker slides from this activity available for use as a non-accredited self-study or teaching resource.
This slide deck presented by Dr. Kami Maddocks, Professor-Clinical in the Division of Hematology and
Associate Division Director for Ambulatory Operations
The Ohio State University Comprehensive Cancer Center, will provide insight into new directions in targeted therapeutic approaches for older adults with mantle cell lymphoma.
STATEMENT OF NEED
Mantle cell lymphoma (MCL) is a rare, aggressive B-cell non-Hodgkin lymphoma (NHL) accounting for 5% to 7% of all lymphomas. Its prognosis ranges from indolent disease that does not require treatment for years to very aggressive disease, which is associated with poor survival (Silkenstedt et al, 2021). Typically, MCL is diagnosed at advanced stage and in older patients who cannot tolerate intensive therapy (NCCN, 2022). Although recent advances have slightly increased remission rates, recurrence and relapse remain very common, leading to a median overall survival between 3 and 6 years (LLS, 2021). Though there are several effective options, progress is still needed towards establishing an accepted frontline approach for MCL (Castellino et al, 2022). Treatment selection and management of MCL are complicated by the heterogeneity of prognosis, advanced age and comorbidities of patients, and lack of an established standard approach for treatment, making it vital that clinicians be familiar with the latest research and advances in this area. In this activity chaired by Michael Wang, MD, Professor in the Department of Lymphoma & Myeloma at MD Anderson Cancer Center, expert faculty will discuss prognostic factors informing treatment, the promising results of recent trials in new therapeutic approaches, and the implications of treatment resistance in therapeutic selection for MCL.
Target Audience
Hematology/oncology fellows, attending faculty, and other health care professionals involved in the treatment of patients with mantle cell lymphoma (MCL).
Learning Objectives
1.) Identify clinical and biological prognostic factors that can guide treatment decision making for older adults with MCL
2.) Evaluate emerging data on targeted therapeutic approaches for treatment-naive and relapsed/refractory MCL and their applicability to older adults
3.) Assess mechanisms of resistance to targeted therapies for MCL and their implications for treatment selection
The prostate is an exocrine gland of the male mammalian reproductive system
It is a walnut-sized gland that forms part of the male reproductive system and is located in front of the rectum and just below the urinary bladder
Function is to store and secrete a clear, slightly alkaline fluid that constitutes 10-30% of the volume of the seminal fluid that along with the spermatozoa, constitutes semen
A healthy human prostate measures (4cm-vertical, by 3cm-horizontal, 2cm ant-post ).
It surrounds the urethra just below the urinary bladder. It has anterior, median, posterior and two lateral lobes
It’s work is regulated by androgens which are responsible for male sex characteristics
Generalised disease of the prostate due to hormonal derangement which leads to non malignant enlargement of the gland (increase in the number of epithelial cells and stromal tissue)to cause compression of the urethra leading to symptoms (LUTS
Title: Sense of Smell
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the primary categories of smells and the concept of odor blindness.
Explain the structure and location of the olfactory membrane and mucosa, including the types and roles of cells involved in olfaction.
Describe the pathway and mechanisms of olfactory signal transmission from the olfactory receptors to the brain.
Illustrate the biochemical cascade triggered by odorant binding to olfactory receptors, including the role of G-proteins and second messengers in generating an action potential.
Identify different types of olfactory disorders such as anosmia, hyposmia, hyperosmia, and dysosmia, including their potential causes.
Key Topics:
Olfactory Genes:
3% of the human genome accounts for olfactory genes.
400 genes for odorant receptors.
Olfactory Membrane:
Located in the superior part of the nasal cavity.
Medially: Folds downward along the superior septum.
Laterally: Folds over the superior turbinate and upper surface of the middle turbinate.
Total surface area: 5-10 square centimeters.
Olfactory Mucosa:
Olfactory Cells: Bipolar nerve cells derived from the CNS (100 million), with 4-25 olfactory cilia per cell.
Sustentacular Cells: Produce mucus and maintain ionic and molecular environment.
Basal Cells: Replace worn-out olfactory cells with an average lifespan of 1-2 months.
Bowman’s Gland: Secretes mucus.
Stimulation of Olfactory Cells:
Odorant dissolves in mucus and attaches to receptors on olfactory cilia.
Involves a cascade effect through G-proteins and second messengers, leading to depolarization and action potential generation in the olfactory nerve.
Quality of a Good Odorant:
Small (3-20 Carbon atoms), volatile, water-soluble, and lipid-soluble.
Facilitated by odorant-binding proteins in mucus.
Membrane Potential and Action Potential:
Resting membrane potential: -55mV.
Action potential frequency in the olfactory nerve increases with odorant strength.
Adaptation Towards the Sense of Smell:
Rapid adaptation within the first second, with further slow adaptation.
Psychological adaptation greater than receptor adaptation, involving feedback inhibition from the central nervous system.
Primary Sensations of Smell:
Camphoraceous, Musky, Floral, Pepperminty, Ethereal, Pungent, Putrid.
Odor Detection Threshold:
Examples: Hydrogen sulfide (0.0005 ppm), Methyl-mercaptan (0.002 ppm).
Some toxic substances are odorless at lethal concentrations.
Characteristics of Smell:
Odor blindness for single substances due to lack of appropriate receptor protein.
Behavioral and emotional influences of smell.
Transmission of Olfactory Signals:
From olfactory cells to glomeruli in the olfactory bulb, involving lateral inhibition.
Primitive, less old, and new olfactory systems with different path
Ethanol (CH3CH2OH), or beverage alcohol, is a two-carbon alcohol
that is rapidly distributed in the body and brain. Ethanol alters many
neurochemical systems and has rewarding and addictive properties. It
is the oldest recreational drug and likely contributes to more morbidity,
mortality, and public health costs than all illicit drugs combined. The
5th edition of the Diagnostic and Statistical Manual of Mental Disorders
(DSM-5) integrates alcohol abuse and alcohol dependence into a single
disorder called alcohol use disorder (AUD), with mild, moderate,
and severe subclassifications (American Psychiatric Association, 2013).
In the DSM-5, all types of substance abuse and dependence have been
combined into a single substance use disorder (SUD) on a continuum
from mild to severe. A diagnosis of AUD requires that at least two of
the 11 DSM-5 behaviors be present within a 12-month period (mild
AUD: 2–3 criteria; moderate AUD: 4–5 criteria; severe AUD: 6–11 criteria).
The four main behavioral effects of AUD are impaired control over
drinking, negative social consequences, risky use, and altered physiological
effects (tolerance, withdrawal). This chapter presents an overview
of the prevalence and harmful consequences of AUD in the U.S.,
the systemic nature of the disease, neurocircuitry and stages of AUD,
comorbidities, fetal alcohol spectrum disorders, genetic risk factors, and
pharmacotherapies for AUD.
Anti ulcer drugs and their Advance pharmacology ||
Anti-ulcer drugs are medications used to prevent and treat ulcers in the stomach and upper part of the small intestine (duodenal ulcers). These ulcers are often caused by an imbalance between stomach acid and the mucosal lining, which protects the stomach lining.
||Scope: Overview of various classes of anti-ulcer drugs, their mechanisms of action, indications, side effects, and clinical considerations.
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Tom Selleck Health: A Comprehensive Look at the Iconic Actor’s Wellness Journeygreendigital
Tom Selleck, an enduring figure in Hollywood. has captivated audiences for decades with his rugged charm, iconic moustache. and memorable roles in television and film. From his breakout role as Thomas Magnum in Magnum P.I. to his current portrayal of Frank Reagan in Blue Bloods. Selleck's career has spanned over 50 years. But beyond his professional achievements. fans have often been curious about Tom Selleck Health. especially as he has aged in the public eye.
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Introduction
Many have been interested in Tom Selleck health. not only because of his enduring presence on screen but also because of the challenges. and lifestyle choices he has faced and made over the years. This article delves into the various aspects of Tom Selleck health. exploring his fitness regimen, diet, mental health. and the challenges he has encountered as he ages. We'll look at how he maintains his well-being. the health issues he has faced, and his approach to ageing .
Early Life and Career
Childhood and Athletic Beginnings
Tom Selleck was born on January 29, 1945, in Detroit, Michigan, and grew up in Sherman Oaks, California. From an early age, he was involved in sports, particularly basketball. which played a significant role in his physical development. His athletic pursuits continued into college. where he attended the University of Southern California (USC) on a basketball scholarship. This early involvement in sports laid a strong foundation for his physical health and disciplined lifestyle.
Transition to Acting
Selleck's transition from an athlete to an actor came with its physical demands. His first significant role in "Magnum P.I." required him to perform various stunts and maintain a fit appearance. This role, which he played from 1980 to 1988. necessitated a rigorous fitness routine to meet the show's demands. setting the stage for his long-term commitment to health and wellness.
Fitness Regimen
Workout Routine
Tom Selleck health and fitness regimen has evolved. adapting to his changing roles and age. During his "Magnum, P.I." days. Selleck's workouts were intense and focused on building and maintaining muscle mass. His routine included weightlifting, cardiovascular exercises. and specific training for the stunts he performed on the show.
Selleck adjusted his fitness routine as he aged to suit his body's needs. Today, his workouts focus on maintaining flexibility, strength, and cardiovascular health. He incorporates low-impact exercises such as swimming, walking, and light weightlifting. This balanced approach helps him stay fit without putting undue strain on his joints and muscles.
Importance of Flexibility and Mobility
In recent years, Selleck has emphasized the importance of flexibility and mobility in his fitness regimen. Understanding the natural decline in muscle mass and joint flexibility with age. he includes stretching and yoga in his routine. These practices help prevent injuries, improve posture, and maintain mobilit
Pulmonary Thromboembolism - etilogy, types, medical- Surgical and nursing man...VarunMahajani
Disruption of blood supply to lung alveoli due to blockage of one or more pulmonary blood vessels is called as Pulmonary thromboembolism. In this presentation we will discuss its causes, types and its management in depth.
Explore natural remedies for syphilis treatment in Singapore. Discover alternative therapies, herbal remedies, and lifestyle changes that may complement conventional treatments. Learn about holistic approaches to managing syphilis symptoms and supporting overall health.
- Video recording of this lecture in English language: https://youtu.be/lK81BzxMqdo
- Video recording of this lecture in Arabic language: https://youtu.be/Ve4P0COk9OI
- Link to download the book free: https://nephrotube.blogspot.com/p/nephrotube-nephrology-books.html
- Link to NephroTube website: www.NephroTube.com
- Link to NephroTube social media accounts: https://nephrotube.blogspot.com/p/join-nephrotube-on-social-media.html
2. Microorganisms of the skin and
mucous membranes
• Microbial flora of the skin and mucous
membranes consist of:
1. Resident flora, usually commensal
microorganisms
2. Pathogenic microorganisms
3. Normal flora of the skin
• Staphylococcus epidermidis
• Staphylococcus aureus (in small numbers)
• Alpha-hemolytic and nonhemolytic Streptococcus
• Micrococcus species
• Peptostreptococcus species
• Neisseriae species ( nonpathogenic )
• Propionibacterium species
• Diphtheroids
• Candida species ( small numbers )
• Acinetobacter species ( small numbers )
4. Normal flora of the skin
• The skin is particularly apt to contain
transient microorganisms, because of its
constant exposure to and contact with the
environment
• There is a constant and well-defined resident
flora, modified in different anatomic area by
secretions, proximity to mucous membranes
(mouth, nose, perineal areas) and habitual
wearing of clothing.
5. Normal flora of the skin
• Neither profuse sweating nor washing and
bathing can eliminate or significantly modify
the normal resident flora
• The number of superficial microorganisms
may be diminished by vigorous daily
scrubbing with soap, but the flora is rapidly
replenished from sebaceous and sweat
glands even when contact with other skin
area or with the environment is completely
excluded.
6. Normal flora of the skin
• Placement of an occlusive dressing on the
skin tends to result in a large increase in the
total microbial population and may also
produce qualitative alterations in the flora.
7. Normal flora of the skin
• Anaerobic and aerobic bacteria often join to
form synergistic infections (gangrene,
necrotizing fasciitis, cellulitis) of skin and soft
tissues
• The bacteria are frequently part of the
normal microbial flora
• It is usually difficult to pinpoint one specific
organism as being responsible for the
progressive lesion, since mixtures of
organisms are usually involved.
8. Role of the resident flora
• Role of resident flora of the skin and mucous
membranes:
– To prevent colonization by pathogens and
possible disease through bacterial interference.
• The mechanisms of bacterial interference :
1.Competition for receptors or binding site on host
cells
2.Competition for nutrients
3.Mutual inhibition by metabolic or toxic products
4.Mutual inhibition by antibiotic materials or
bacteriocins.
9. Role of the resident flora
• The factors that may be important in
eliminating nonresident microorganism
from the skin are the low pH, the fatty acid
in sebaceous secretions, and the
presence of lyzozyme.
• Suppression of the normal flora creates a
partial local void that tend to be filled by
microorganisms from the environment or
from other part of the body.
10. Role of the resident flora
• Members of the normal flora may themselves
produce disease under certain
circumstances.
• Such organisms behaves as opportunists
and may then become pathogens.
• These organisms are adapted to the
noninvasive mode of life defined by the
limitations of the environment.
11. Role of the resident flora
• If we forcefully remove the restrictions of that
environment and they are introduced into the
blood stream or tissues, these organisms
may become pathogenic.
– Eg. Large numbers of Streptococcus viridans
(normal flora of the upper respiratory tract)
introduced into bloodstream (following tooth
extraction or tonsillectomy), they may settle on
deformed heart valve and produce infective
endocarditis.
13. Normal flora of the intestinal
tract
• At birth the intestine is sterile, but
organisms are soon introduced with food.
• Bowels of newborns in intensive care
nurseries tend to be colonized by
Enterobacteriaceae, e.g. Klebsiella,
Citrobacter, Enterobacter.
• Diet has a marked influence on the
relative composition of the intestinal fecal
flora.
14. Normal flora of the intestinal
tract
• In breast-fed children,
– the intestine contain large numbers of lactic acid
streptococci and lactobacilli. These aerobic and
anaerobic, gram positive, nonmotile organisms
(e.g. Bifidobacterium species) produced acid
from carbohydrates and tolerate pH 5.0
• In bottle-fed children,
– a more mixed flora exist in the bowel, and
lactobacilli are less prominent. As food habits
develop toward the adult pattern, the bowel flora
changes.
15. Normal flora of the intestinal
tract
• In normal adults, the esophagus contains
microorganisms arriving with saliva and food.
• The stomach’s acidity keep the number of
microorganisms at minimum (103 – 105 /gr
content ) unless obstruction at pylorus favors
the proliferation of gram +ve cocci & bacilli.
• The normal acid pH of the stomach markedly
protects against infection with some enteric
pathogens, e.g. cholera.
16. Normal flora of the intestinal
tract
• Administration of H2 receptor blockers like
cimetidine for peptic ulcer leads to great
increase in microbial flora of the stomach,
including many organisms usually prevalent
in feces.
17. Normal flora of the intestinal
tract
• As the pH of intestinal content become
alkaline, the resident flora gradually
increases.
– In the adult duodenum, there are 103 -106
bacteria per gram of content;
– In the jejunum and ileum, 105-108 bacteria/gr;
and
– In the cecum and transverse colon, 108-1010
bacteria/gr
18. Normal flora of the intestinal
tract
• As the pH of intestinal content become
alkaline, the resident flora gradually
increases.
– In the upper intestine, lactobacilli and
enterococci predominate, but in the lower
ileum and cecum, the flora is fecal
– In the sigmoid colon and rectum, there are
about 1011 bacteria/gr of content, constituting
10 – 30% of the fecal mass
19. Normal flora of the intestinal
tract
• Anaerobes outnumber facultative organisms
by 1000-fold. In diarrhea the bacterial
content may diminish greatly, whereas in
intestinal stasis the count rises
• In the normal adult colon, 96 – 99% of the
resident bacterial flora consists of anaerobes
– Bacteroides sp, especially B. fragilis
– Fusobacterium sp
– Anaerobic lactobacilli, e.g. bifidobacteria
– Clostridia ( C.perfringens, 103 -105/gr)
– Anaerobic gram positive cocci (Peptostreptococcus sp)
20. Normal flora of the intestinal
tract
• Only 1 – 4% are facultative aerobes:
– Gram negative coliform bacteria
– Enterococci
– Small number of protei, pseudomonads,
lactobacilli, candidae
– More than 100 distinct types of organisms,
which can be cultured routinely in the
laboratory, occur regularly in the normal fecal
flora
21. Normal flora of the intestinal
tract
• There probably are more than 500 sp of
bacteria in the colon including many that
are likely unidentified.
• Minor trauma(e.g. sigmoidoscopy, barium
enema) may induce transient bacteremia
in about 10% of procedures
22. Normal flora of the intestinal
tract
• Intestinal bacteria are important in :
– Synthesis of vitamin K
– Conversion of bile pigments and bile acids
– Absorption of nutrients and breakdown products
– Antagonism to microbial pathogens
• The intestinal flora produces ammonia and
other breakdown products that are absorbed
and can contribute to hepatic coma
– Among aerobic coliform bacteria, only few serotypes persist
in the colon for prolonged periods, and most serotypes of
Escherichia coli are present only over period of a few days
23. Normal flora of the intestinal
tract
• Antimicrobial drugs taken orally can, in
human, temporarily suppress the drug
susceptible component of the fecal flora
• This is commonly done by preoperative oral
administration of insoluble drug.
– For example, neomycin plus erythromycin can in
1 – 2 days suppress part of the bowel flora,
especially aerobes
24. Normal flora of the intestinal
tract
• Metronidazole accomplishes that for
anaerobes.
– If lower bowel surgery is performed when the
counts are at their lowest, some protection
against infection by accidental spill can be
achieved
• However, soon thereafter the counts of fecal
flora rise again to normal or higher than
normal levels, principally of organisms
selected out because to relative resistance to
the drug employed.
25. Normal flora of the intestinal
tract
• Drug susceptible microorganisms are
replaced by drug resistant ones, particularly
staphylococci, enterobacters, enterococci,
protei, pseudomonads, Clostridium difficile
and yeast.
• The anaerobic flora of the colon, including B.
fragilis, clostridia and peptostreptococci play
a main role in abscess formation originating
in perforation of the bowel.
26. Normal flora of the intestinal
tract
• Prevotella bivia, P. disiens are important in
the abscesses of the pelvis originating in the
female genital organ. These species are
penicillin-resistant
• The feeding of large quantities of
Lactobacillus acidophilus may result in the
temporary establishment of these organisms
in the gut and the concomitant partial
suppression of other gut microflora.
28. Normal physiology of vagina
• Normal physiology of vaginal discharge
include:
– Cervical & vaginal epithelial cells,
– Normal bacteria flora ,
– Water,
– Electrolytes and
– Other chemicals.
29. Normal flora of vagina
• The normal vaginal vaginal flora often
includes also alpha hemolytic streptococci,
anaerobic streptococci( peptostreptococci),
Prevotella sp , clostridia, Gardnerella
vaginalis, Ureaplasma urealyticum, and
sometimes listeria or Mobiluncus sp
• Normal vaginal pH varies from 4 – 4.5.
30. Normal flora of the vagina
• Soon after birth, aerobic lactobacilli appear in
the vagina and persist as long as the pH
remains acids (several weeks).
• When pH become neutral (remaining so until
puberty), a mixed flora of cocci and bacilli is
present.
• During birth, Vaginal organisms present at
time of delivery may infect the newborn (e.g.
group B streptococci) that subsequently
leads to neonatal sepsis and meningitis.
31. Normal flora of the vagina
• At puberty, aerobic and anaerobic lactobacilli
reappear in large numbers and contribute to
the maintenance of acid pH through the
production of acid from carbohydrates,
particularly glycogen.
• This appears to be an important
mechanisms in preventing the establishment
of other, possibly harmful microorganism in
the vagina
32. Normal vaginal response
• Lacto bacillus produces lactic acid fatty acid
and organic acid .
• Glucose is the source of organism. After
menopause as there is decrease in glucose
availability in the vaginal secretions, there is
a decreased substrate for acid production.
• This lead to increase vaginal pH.
• FSH has direction relation with vaginal pH
while estradiol has inverse relation.
33. Normal flora of the vagina
• If lactobacilli are suppressed by the
administration of antimicrobial drugs,
yeast or various bacteria increase in
numbers and cause irritation and
inflammation
• After menopause, lactobacilli again
diminish in number and mixed flora
returns.
• The normal vaginal flora includes group B
streptococci in as many as 25% of women
34. Vaginal infections
• The cervical mucus has antibacterial activity
and contain lyzozyme that reduces chances
of infection.
• In some women, the vaginal introitus contain
a heavy flora resembling that the perineum
and perianal area
• This may be a predisposing factor in
recurrent urinary tract infections
35. Vaginal infections
• Vaginal infection are often (varies between
countries between 20 to 40% of vaginal
infections) a mix of various etiologies, which
present challenging cases for treatment.
• Indeed, when only one cause is treated, the
other pathogens can gain in resistance and
induce relapses and recurrences.
• The key factor is therefore to get a precise
diagnosis and treat with broad spectrum anti-
infective (often inducing adverse effects).
37. Bacterial vaginosis
• Most common vaginal infection in women
of reproductive age.
• It reflects a shift in vaginal flora from
lactobacilli dominant to mixed flora (genital
microplasma ,g.vaginalis , anaerobes)
• no causative agent has been identified.
• Due absence of inflammation in biopsy,
hence the term vaginosis rather than
vaginitis
39. Normal flora of the urethra
• The anterior urethra of both sexes
contains small numbers of the same types
of organisms found on the skin and
perineum
• This organism regularly appear in normal
voided urine in numbers of 102 – 104/mL