A microscope is an instrument used to observe very small organisms i.e. microorganisms. The microscope provides magnification and resolution which makes the image enlarged and fine. There are different types of microscopes ranging from simple to compound microscopes.
as a partial requirement for one of my subject for this semester
I would like you to view my presentation and comment as well
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as a partial requirement for one of my subject for this semester
I would like you to view my presentation and comment as well
I will be very glad if you find my presentation interesting, or comment on how I can improve my craft, THANK YOU :)
Types of Microscopes with their applications - Microbiologynote.com
https://microbiologynote.com/types-of-microscopes-with-their-applications/
Youtube Lecture Video:
https://www.youtube.com/watch?v=nuJZtXohFFQ&ab_channel=MicrobiologyNote
Compound microscopes are what most people visualize when they think about microscopes. They are available in monocular, binocular and trinocular formats. They have a number of objectives (the lens closest to the object being viewed) of varying magnifications mounted in a rotating nosepiece.
Microscopy - Magnification, Resolving power, Principles, Types and ApplicationsNethravathi Siri
Magnification, Resolving power, Principles and Applications of Simple, Compound, Stereozoom, Phase contrast, Fluorescent and Electron microscopes (TEM & SEM).
Microscopy is the technical field that uses microscopes to observe samples which are not in the resolution range of the normal-unaided eye.
Types of Microscopes with their applications - Microbiologynote.com
https://microbiologynote.com/types-of-microscopes-with-their-applications/
Youtube Lecture Video:
https://www.youtube.com/watch?v=nuJZtXohFFQ&ab_channel=MicrobiologyNote
Compound microscopes are what most people visualize when they think about microscopes. They are available in monocular, binocular and trinocular formats. They have a number of objectives (the lens closest to the object being viewed) of varying magnifications mounted in a rotating nosepiece.
Microscopy - Magnification, Resolving power, Principles, Types and ApplicationsNethravathi Siri
Magnification, Resolving power, Principles and Applications of Simple, Compound, Stereozoom, Phase contrast, Fluorescent and Electron microscopes (TEM & SEM).
Microscopy is the technical field that uses microscopes to observe samples which are not in the resolution range of the normal-unaided eye.
INTRODUCTION
HISTORY
TYPES OF MICROSCOPE
Compound Microscope
Dissection Microscope
Scanning Electron Microscope (SEM)
Transmission Electron Microscope (TEM
CARE
PARTS AND FUNCTION
FOCUSING
CONCLUSION
REFERANCE
In the late 16th century several Dutch lens makers designed devices that magnified objects, but in 1609 Galileo Galilei perfected the first device known as a microscope. Dutch spectacle makers Zaccharias Janssen and Hans Lipperhey are noted as the first men to develop the concept of the compound microscope.
Microscopy is the technique of using microscopes to observe and analyze objects that are too small to be seen by the naked eye. Microscopes are instruments that magnify and resolve the details of objects, allowing scientists and researchers to study the structure, composition, and behavior of materials and specimens at a microscopic level
Microscopy is the technique of using microscopes to observe and analyze objects that are too small to be seen by the naked eye. Microscopes are instruments that magnify and resolve the details of objects, allowing scientists and researchers to study the structure, composition, and behavior of materials and specimens at a microscopic level
Adenoviral conjunctivitis, also known as "pink eye is a common, self-limiting condition that typically is caused by adenovirus.
Adenoviruses can cause an array of clinical diseases, including conjunctivitis, respiratory disease, hemorrhagic cystitis and gastroenteritis.
Bacteria are unicellular, procaryotic microorganisms which have diverse shape size and structures. Bacteria are found almost everywhere on Earth. Even the human body is full of bacteria, and in fact is estimated to contain more bacterial cells than human cells. Most bacteria in the body are harmless, and some are even helpful. A relatively small number of species cause disease.
Mitochondria are membrane-bound cell organelles (mitochondrion, singular), known as the power house of the cell that generate most of the chemical energy needed to power the cell's biochemical reactions. Mitochondria generates most of the cell's supply of adenosine triphosphate (ATP), by a process called
“oxidative phosphorylation”.
Type II Hypersensitivity-Antibody mediated cytotoxic HypersensitivityAnup Bajracharya
Type II Hypersensitivity is antibody-mediated immune reaction in which antibodies (IgG or IgM) are directed against cellular or extracellular matrix antigens with the resultant cellular destruction, functional loss, or damage to tissues.
Dark-field microscopy is used to illuminate unstained samples causing them to appear bright against a dark background. This type of microscope contains a special condenser having a central blacked-out area.
Air pollution Basics for higher level school students - Undesirable and unwanted changes in the characteristics of air that are harmful to the health of humans and other living beings is air pollution.
Pentose phosphate pathway is an alternative pathway to glycolysis and TCA cycle for oxidation of glucose. It is a shunt of glycolysis. It is also known as hexose monophosphate (HMP) shunt or phosphogluconate pathway. It occurs in cytoplasm of both prokaryotes and eukaryotes. While it involves oxidation of glucose, its primary role is anabolic rather than catabolic. It is an important pathway that generates precursors for nucleotide synthesis and is especially important in red blood cells (erythrocytes).
It is the catabolic process by which fatty acid molecules are broken down in the cytosol in prokaryotes and in the mitochondria in eukaryotes to generate acetyl-CoA.
Environmental impacts of coronavirus crisis, challenges aheadAnup Bajracharya
The worldwide disruption caused by the COVID-19 pandemic has resulted in numerous impacts on the environment and the climate. There is reduction in carbon emissions, visibility clearance in the environment.
Convalescent plasma therapy is the treatment in which plasma that is removed from the blood of a person who has recovered from a disease is transfused into a person with the disease that fight against the causative agents and make patient safe from the diseased condition.
Acid fast staining is differential staining technique which differentiate bacteria into two group- acid fast bacteria and non acid bacteria. It used to identify acid-fast organisms such as members of the genus Mycobacterium .
When fresh liquid medium is inoculated with a given number of bacteria and incubated for sufficient period of time, it gives a characteristic growth pattern of bacteria.
If the bacterial population is measured periodically and log of number of viable bacteria is plotted in a graph against time, it gives a characteristic growth curve which is known as growth curve or growth cycle.
Growth of bacteria is affected by many factors such as nutrition concentration and other environmental factors.
Some of the important factors affecting bacterial growth are:
Nutrition concentration
Temperature
Gaseous concentration
pH
Ions and salt concentration
Available water
Tom Selleck Health: A Comprehensive Look at the Iconic Actor’s Wellness Journeygreendigital
Tom Selleck, an enduring figure in Hollywood. has captivated audiences for decades with his rugged charm, iconic moustache. and memorable roles in television and film. From his breakout role as Thomas Magnum in Magnum P.I. to his current portrayal of Frank Reagan in Blue Bloods. Selleck's career has spanned over 50 years. But beyond his professional achievements. fans have often been curious about Tom Selleck Health. especially as he has aged in the public eye.
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Introduction
Many have been interested in Tom Selleck health. not only because of his enduring presence on screen but also because of the challenges. and lifestyle choices he has faced and made over the years. This article delves into the various aspects of Tom Selleck health. exploring his fitness regimen, diet, mental health. and the challenges he has encountered as he ages. We'll look at how he maintains his well-being. the health issues he has faced, and his approach to ageing .
Early Life and Career
Childhood and Athletic Beginnings
Tom Selleck was born on January 29, 1945, in Detroit, Michigan, and grew up in Sherman Oaks, California. From an early age, he was involved in sports, particularly basketball. which played a significant role in his physical development. His athletic pursuits continued into college. where he attended the University of Southern California (USC) on a basketball scholarship. This early involvement in sports laid a strong foundation for his physical health and disciplined lifestyle.
Transition to Acting
Selleck's transition from an athlete to an actor came with its physical demands. His first significant role in "Magnum P.I." required him to perform various stunts and maintain a fit appearance. This role, which he played from 1980 to 1988. necessitated a rigorous fitness routine to meet the show's demands. setting the stage for his long-term commitment to health and wellness.
Fitness Regimen
Workout Routine
Tom Selleck health and fitness regimen has evolved. adapting to his changing roles and age. During his "Magnum, P.I." days. Selleck's workouts were intense and focused on building and maintaining muscle mass. His routine included weightlifting, cardiovascular exercises. and specific training for the stunts he performed on the show.
Selleck adjusted his fitness routine as he aged to suit his body's needs. Today, his workouts focus on maintaining flexibility, strength, and cardiovascular health. He incorporates low-impact exercises such as swimming, walking, and light weightlifting. This balanced approach helps him stay fit without putting undue strain on his joints and muscles.
Importance of Flexibility and Mobility
In recent years, Selleck has emphasized the importance of flexibility and mobility in his fitness regimen. Understanding the natural decline in muscle mass and joint flexibility with age. he includes stretching and yoga in his routine. These practices help prevent injuries, improve posture, and maintain mobilit
- Video recording of this lecture in English language: https://youtu.be/lK81BzxMqdo
- Video recording of this lecture in Arabic language: https://youtu.be/Ve4P0COk9OI
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Flu Vaccine Alert in Bangalore Karnatakaaddon Scans
As flu season approaches, health officials in Bangalore, Karnataka, are urging residents to get their flu vaccinations. The seasonal flu, while common, can lead to severe health complications, particularly for vulnerable populations such as young children, the elderly, and those with underlying health conditions.
Dr. Vidisha Kumari, a leading epidemiologist in Bangalore, emphasizes the importance of getting vaccinated. "The flu vaccine is our best defense against the influenza virus. It not only protects individuals but also helps prevent the spread of the virus in our communities," he says.
This year, the flu season is expected to coincide with a potential increase in other respiratory illnesses. The Karnataka Health Department has launched an awareness campaign highlighting the significance of flu vaccinations. They have set up multiple vaccination centers across Bangalore, making it convenient for residents to receive their shots.
To encourage widespread vaccination, the government is also collaborating with local schools, workplaces, and community centers to facilitate vaccination drives. Special attention is being given to ensuring that the vaccine is accessible to all, including marginalized communities who may have limited access to healthcare.
Residents are reminded that the flu vaccine is safe and effective. Common side effects are mild and may include soreness at the injection site, mild fever, or muscle aches. These side effects are generally short-lived and far less severe than the flu itself.
Healthcare providers are also stressing the importance of continuing COVID-19 precautions. Wearing masks, practicing good hand hygiene, and maintaining social distancing are still crucial, especially in crowded places.
Protect yourself and your loved ones by getting vaccinated. Together, we can help keep Bangalore healthy and safe this flu season. For more information on vaccination centers and schedules, residents can visit the Karnataka Health Department’s official website or follow their social media pages.
Stay informed, stay safe, and get your flu shot today!
These lecture slides, by Dr Sidra Arshad, offer a quick overview of physiological basis of a normal electrocardiogram.
Learning objectives:
1. Define an electrocardiogram (ECG) and electrocardiography
2. Describe how dipoles generated by the heart produce the waveforms of the ECG
3. Describe the components of a normal electrocardiogram of a typical bipolar leads (limb II)
4. Differentiate between intervals and segments
5. Enlist some common indications for obtaining an ECG
Study Resources:
1. Chapter 11, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 9, Human Physiology - From Cells to Systems, Lauralee Sherwood, 9th edition
3. Chapter 29, Ganong’s Review of Medical Physiology, 26th edition
4. Electrocardiogram, StatPearls - https://www.ncbi.nlm.nih.gov/books/NBK549803/
5. ECG in Medical Practice by ABM Abdullah, 4th edition
6. ECG Basics, http://www.nataliescasebook.com/tag/e-c-g-basics
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Ve...kevinkariuki227
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Verified Chapters 1 - 19, Complete Newest Version.pdf
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Verified Chapters 1 - 19, Complete Newest Version.pdf
Title: Sense of Taste
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the structure and function of taste buds.
Describe the relationship between the taste threshold and taste index of common substances.
Explain the chemical basis and signal transduction of taste perception for each type of primary taste sensation.
Recognize different abnormalities of taste perception and their causes.
Key Topics:
Significance of Taste Sensation:
Differentiation between pleasant and harmful food
Influence on behavior
Selection of food based on metabolic needs
Receptors of Taste:
Taste buds on the tongue
Influence of sense of smell, texture of food, and pain stimulation (e.g., by pepper)
Primary and Secondary Taste Sensations:
Primary taste sensations: Sweet, Sour, Salty, Bitter, Umami
Chemical basis and signal transduction mechanisms for each taste
Taste Threshold and Index:
Taste threshold values for Sweet (sucrose), Salty (NaCl), Sour (HCl), and Bitter (Quinine)
Taste index relationship: Inversely proportional to taste threshold
Taste Blindness:
Inability to taste certain substances, particularly thiourea compounds
Example: Phenylthiocarbamide
Structure and Function of Taste Buds:
Composition: Epithelial cells, Sustentacular/Supporting cells, Taste cells, Basal cells
Features: Taste pores, Taste hairs/microvilli, and Taste nerve fibers
Location of Taste Buds:
Found in papillae of the tongue (Fungiform, Circumvallate, Foliate)
Also present on the palate, tonsillar pillars, epiglottis, and proximal esophagus
Mechanism of Taste Stimulation:
Interaction of taste substances with receptors on microvilli
Signal transduction pathways for Umami, Sweet, Bitter, Sour, and Salty tastes
Taste Sensitivity and Adaptation:
Decrease in sensitivity with age
Rapid adaptation of taste sensation
Role of Saliva in Taste:
Dissolution of tastants to reach receptors
Washing away the stimulus
Taste Preferences and Aversions:
Mechanisms behind taste preference and aversion
Influence of receptors and neural pathways
Impact of Sensory Nerve Damage:
Degeneration of taste buds if the sensory nerve fiber is cut
Abnormalities of Taste Detection:
Conditions: Ageusia, Hypogeusia, Dysgeusia (parageusia)
Causes: Nerve damage, neurological disorders, infections, poor oral hygiene, adverse drug effects, deficiencies, aging, tobacco use, altered neurotransmitter levels
Neurotransmitters and Taste Threshold:
Effects of serotonin (5-HT) and norepinephrine (NE) on taste sensitivity
Supertasters:
25% of the population with heightened sensitivity to taste, especially bitterness
Increased number of fungiform papillae
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Report Back from SGO 2024: What’s the Latest in Cervical Cancer?bkling
Are you curious about what’s new in cervical cancer research or unsure what the findings mean? Join Dr. Emily Ko, a gynecologic oncologist at Penn Medicine, to learn about the latest updates from the Society of Gynecologic Oncology (SGO) 2024 Annual Meeting on Women’s Cancer. Dr. Ko will discuss what the research presented at the conference means for you and answer your questions about the new developments.
Title: Sense of Smell
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the primary categories of smells and the concept of odor blindness.
Explain the structure and location of the olfactory membrane and mucosa, including the types and roles of cells involved in olfaction.
Describe the pathway and mechanisms of olfactory signal transmission from the olfactory receptors to the brain.
Illustrate the biochemical cascade triggered by odorant binding to olfactory receptors, including the role of G-proteins and second messengers in generating an action potential.
Identify different types of olfactory disorders such as anosmia, hyposmia, hyperosmia, and dysosmia, including their potential causes.
Key Topics:
Olfactory Genes:
3% of the human genome accounts for olfactory genes.
400 genes for odorant receptors.
Olfactory Membrane:
Located in the superior part of the nasal cavity.
Medially: Folds downward along the superior septum.
Laterally: Folds over the superior turbinate and upper surface of the middle turbinate.
Total surface area: 5-10 square centimeters.
Olfactory Mucosa:
Olfactory Cells: Bipolar nerve cells derived from the CNS (100 million), with 4-25 olfactory cilia per cell.
Sustentacular Cells: Produce mucus and maintain ionic and molecular environment.
Basal Cells: Replace worn-out olfactory cells with an average lifespan of 1-2 months.
Bowman’s Gland: Secretes mucus.
Stimulation of Olfactory Cells:
Odorant dissolves in mucus and attaches to receptors on olfactory cilia.
Involves a cascade effect through G-proteins and second messengers, leading to depolarization and action potential generation in the olfactory nerve.
Quality of a Good Odorant:
Small (3-20 Carbon atoms), volatile, water-soluble, and lipid-soluble.
Facilitated by odorant-binding proteins in mucus.
Membrane Potential and Action Potential:
Resting membrane potential: -55mV.
Action potential frequency in the olfactory nerve increases with odorant strength.
Adaptation Towards the Sense of Smell:
Rapid adaptation within the first second, with further slow adaptation.
Psychological adaptation greater than receptor adaptation, involving feedback inhibition from the central nervous system.
Primary Sensations of Smell:
Camphoraceous, Musky, Floral, Pepperminty, Ethereal, Pungent, Putrid.
Odor Detection Threshold:
Examples: Hydrogen sulfide (0.0005 ppm), Methyl-mercaptan (0.002 ppm).
Some toxic substances are odorless at lethal concentrations.
Characteristics of Smell:
Odor blindness for single substances due to lack of appropriate receptor protein.
Behavioral and emotional influences of smell.
Transmission of Olfactory Signals:
From olfactory cells to glomeruli in the olfactory bulb, involving lateral inhibition.
Primitive, less old, and new olfactory systems with different path
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2. What is microscope?
• "Micro" refers to tiny,
• "scope" refers to view or look at.
Microscopes are tools used to enlarge small
objects so as they can be observed and
studied.
Microscopes range from a simple magnifying
glass to the expensive electron microscope.
3. History of Microscopes
• Timeline of the Microscope
• 14th century: spectacles first made in Italy
• 1590: Two Dutch spectacle-makers and father-and-son team, Hans
and Zacharias Janssen, create the first microscope.
• 1667: Robert Hooke's famous "Micrographia" is published, which
outlines Hooke's various studies using the microscope.
• 1675: Anton van Leeuwenhoek, who used a microscope with one
lens to observe insects and other specimen. Leeuwenhoek was the
first to observe bacteria.
• 1830: Joseph Jackson Lister discovers that using weak lenses
together at various distances provided clear magnification.
4. • 1878: A mathematical theory linking resolution to light
wavelength is invented by Ernst Abbe.
• 1903: Richard Zsigmondy invents the ultra microscope,
which allows for observation of specimens below the
wavelength of light.
• 1932: Transparent biological materials are studied for
the first time using Frits Zernike’s invention of the
phase-contrast microscope.
• 1938: Just six years after the invention of the phase
contrast microscope comes the electron microscope,
developed by Ernst Ruska, who realized that using
electrons in microscopy enhanced resolution.
• 1981: 3-D specimen images possible with the invention
of the scanning tunneling microscope by Gerd
Binnig and Heinrich Rohrer.
History of Microscopes
Ernst Ruska
5. Classification of Microscopes
Simple microscope Compound microscope
1. It has a single lens system. 1. It has two or more lens system.
2. Eg:-Magnifying glass. 2. Eg:-Compound microscope
basically used in the laboratory.
Microscope can be classified on the basis of various properties.
On the basis of lens system, microscopes are of two types.
6. Light Microscope Electron Microscope
1. It uses light as a source of
illumination.
1. It uses electron or electron gun as a source of
illumination.
2. Lens used in glass lens 2. Lens used is electromagnetic lens.
3. Here, vacuum is not needed. 3. Vacuum must be created since electrons can
travel only in vacuum.
4. Resolution is low comparing to the
electron microscope.
4. Resolution is very high capable of viewing
particles of size 0.1 to 0.2 nanometer (nm).
5. Image can be directly seen by human
eye.
5. Since, electrons can’t be seen by human eye.
Electrons are converted into an amazing image by
striking in fluorescent screen.
Classification of Microscopes
9. Bright-Field Microscopy
The standard instrument used in the
laboratory to observe microorganisms is the
bright field compound microscope.
In bright field microscopy, the microscopic field
is bright and the microorganisms appear dark
because they absorb some of the light.
10. Principle of Microscopy
• 1. Magnification
The microscope produces an enlarged image of the
object which is examined through it. This enlargement is
known as its magnification and is measured in diameter
• Eg: A magnifying lens which gives an image 36 time as
large as that of the object is said to have a magnification
of 36 diameter or 36X(x=times).
11. Which of these images would
be viewed at a higher power of
magnification?
1.Magnification
We can see better details with higher the powers
of magnification, but we cannot see as much of
the image.
14. 2. Resolution
• The ability of microscope to distinguish two closely spaced
objects as separate and distinct entities called resolution.
• Eg. The human eyes has the resolving power of 0.25mm
which means that two dots is placed 0.25mm apart can be
distinguished as two dots.
• If the distance between the two dots is less than 0.25,only
one dot will be seen.
• Resolving Power(R.P.) =
𝜆
2.𝑁.𝐴
whereλ wavelength
of light ,N.A=Numerical Aperture
15. Resolution : Resolution is the quality of image that is measured
by the smallest distance which could be seen clearly without
the blur due to diffraction.
Resolving power :
The ability of an optical instrument to separate or distinguish
small or closely adjacent objects through the image formation
is said to be resolving power of the instrument.
16.
17.
18. Oil immersion
• Immersion Oil is a special oil used
in microscope work with the
highest power objective lenses (ie
100x lens).
• In light microscopy, oil
immersion is a technique used to
increase the resolving power of
a microscope.
• This is achieved by immersing
both the objective lens and the
specimen in a transparent oil of
high refractive index, thereby
increasing the numerical
aperture of the objective lens.
21. Parts of Microscope
• Body: the portion of the microscope
which contains the lenses.
• Revolving Nosepiece: the assembly
which contains the objective lenses.
• Stage: the platform upon which the
object to be examined is placed
• Sub-stage Assembly: the parts below
the stage; these include the condenser,
iris diaphragm, and illuminator.
• Condenser Lens: a lens assembly
beneath the stage which concentrates
light on the object being examined.
• Diaphragm: a series of holes which
control the amount of light which
reaches the object being examined.
22. • Coarse and Fine
Adjustment Knobs: these
knobs adjust the distance
between the objective lens
and the stage. This distance
is called the working
distance.
• Illuminator: the light
source. It may be built into
the base or it may not be
part of the scope at all.
Parts of Microscope
COARSE ADJUSTMENT KNOB —
rapid control which allows for
quick focusing by moving the
objective lens or stage up and
down. It is used for initial focusing.
FINE ADJUSTMENT KNOB —
Slow but precise control used to
fine focus the image when viewing
at the higher magnifications.
23. Handle and care of Microscope
Always keep your microscope covered when
not in use even if the microscope is stored in a
cabinet.
Always use two hands when moving the
microscope. When carrying your microscope,
hold it by the base and the metal support arm.
24. If using immersion oil, always ensure the objectives are cleaned
immediately after use. Objective, eyepieces and condenser may be
removed for cleaning. Use only lens paper and lens cleaner.
25. Turn the microscope off after use.
Do not keep the light on all day as this will
shorten the bulb’s life.