Light microscopy uses visible light and magnifying lenses to examine small objects not visible to the naked eye. There are several types of light microscopes, including bright-field, dark-field, phase-contrast, fluorescence, and differential interference contrast microscopes. These microscopes employ different optical techniques to produce high-contrast images of cells and intracellular structures that provide insight into biology.

1. Light
Microscopy
PRESENTED BY:
HIMANSHU SAINI

2. Light microscopy
A light microscope is an
instrument that uses visible
light and magnifying lenses
to examine small objects not
visible to the naked eye.
Magnification, however is
not the most important issue
in microscopy. The

3. The light microscopy is so called
because it employs visible light to
detect small objects, is probably the
most well-known and well-used
research tool in biology.
The smallest objects that are
considered to be living are the
bacteria. The smallest bacteria can
be observed and cell shaped
recognized at a mere 100x
magnification. They are invisible in
bright field microscopes, though.

4. The Light Microscope
many types
bright-field microscope
dark-field microscope
phase-contrast microscope
fluorescence microscopes
are compound microscopes
image formed by action of
4

5. The Bright-Field Microscope
produces a dark image
against a brighter
background
has several objective
lenses
parfocal microscopes
remain in focus when
objectives are changed
5

6. 6

7. 7

8. The Dark-Field Microscope
produces a bright
image of the object
against a dark
background
used to observe
living, unstained
8

9. 9

10. The Phase-Contrast
Microscope
 enhances the contrast between
intracellular structures
having slight differences in
refractive index
 excellent way to observe
living cells
10

11. 11

12. 12

13. The Differential Interference
Contrast Microscope
creates image by detecting
differences in refractive
indices and thickness of
different parts of
specimen
excellent way to observe
13

14. The Fluorescence
Microscope
exposes specimen to
ultraviolet, violet, or blue
light
specimens usually stained
with fluorochromes
shows a bright image of
the object resulting from
14

15. 15