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METABOLISM
DR. Jayesh Patidar
www.drjayeshpatidar.blogspot.com
1
Overview of Energy and Metabolism
1. The food we eat, (carbohydrates, lipids,
proteins, and Nucleic Acids), are our only
source of energy for doing the biological work
of cells.
2. All molecules (nutrient molecules included)
have stored (potential) energy in the bonds
between their atoms.
3. The energy the runs most biological Systems
on earth comes from solar energy
4. Plants trap solar energy via the metabolic
reactions of Photosynthesis by producing these
molecules
2www.drjayeshpatidar.blogspot.com
Overview of Energy and Metabolism
Terms
Calorie: basic unit of measurement of energy in
biological system. Indicated by a “c”
In human metabolism Calorie is really kilocalorie or 1,000
calories. Indicated by a “C”
This calorie “C” is the amount of energy required to raise
the temperature to 1 kg of water 1 degree C.
The Caloric needs of most organisms is measured as Basal
Metabolic Rate (BMR)
3www.drjayeshpatidar.blogspot.com
Overview of Energy and Metabolism
Terms
BMR is the minimum resting energy expenditures by an
awake alert person
Average BMR: 70 C/hr or 1680 C/day
If we assume that average amounts of carbohydrates,
lipid, and proteins are being catabolized we can
calculate a ratio of 4.825 “C” per liter of O2 consumed.
BMR in influence by age, gender, physical condition, body
weight, and genes.
4www.drjayeshpatidar.blogspot.com
Three Basic Uses of Nutrients
Absorbed by the Digestive System
1. Energy for immediate use by cells to conduct
their normal Metabolic processes (Muscle
contraction, secretions, active transport)
2. Synthesize structural or functional molecules
to repair and replace cells.
(Mitosis and Cytokinesis)
3. Storage as glycogen or fat for later use as
energy (nutrient reserves)
5www.drjayeshpatidar.blogspot.com
ALL Living Things from Bacteria to
Humans Conduct METABOLISM
Metabolism is the ability to acquire and use energy
from the environment.
Metabolic processes are all the chemical
reactions that occur in cells, tissues, organs,
and organ systems.
Two Kinds of Metabolic Reactions:
1. Catabolism = breakdown of large molecules into
simple ones to produce energy. (release energy)
2. Anabolism = build large molecules from simple
molecules. (requires energy input)
6www.drjayeshpatidar.blogspot.com
Two Basic Kinds of Chemical Reactions
found in Biological System
7www.drjayeshpatidar.blogspot.com
Metabolic Turnover and Cellular ATP
Production
8www.drjayeshpatidar.blogspot.com
Carbohydrate Metabolism
Amino acid synthesis: Convert pyruvic Acid into an
Amino Acid requires a transamination
Transamination: Pyruvic Acid + NH3 Amino Acid
Glycogenesis: glucose glycogen
Lipogenesis: glucose Triglyceride
Glycogenolysis: glycogen glucose
Gluconeogenesis: lactic acid, amino acids, or
triglycerides glucose
9www.drjayeshpatidar.blogspot.com
Summary of Glucose Metabolism
10www.drjayeshpatidar.blogspot.com
Summary of Gluconeogenesis
11www.drjayeshpatidar.blogspot.com
ATP production from Carbohydrates
Glycolysis
Substrates required: Glucose, 2 ATP, 4 ADP,
and 2 NAD+
Intermediate Reactants: Glucose-6-phosphate,
Fructose-1,6
bisphosphate
Products: 2 molecules of Pyruvic Acid
2 ATP
2 NADH 12www.drjayeshpatidar.blogspot.com
Overview of Cellular respiration
13www.drjayeshpatidar.blogspot.com
ATP production from Carbohydrates
Formation of Acetyl-CoA
Substrates required: 2 Pyruvic Acid
2 NAD+
2 Coenzyme A
Products: 2 Acetyl-CoA
2 NADH
2 CO2
14www.drjayeshpatidar.blogspot.com
Overview of Cellular respiration
15www.drjayeshpatidar.blogspot.com
ATP production from Carbohydrates
Kreb’s cycle
Substrates required: 2 Oxaloacetic Acid
2 Acetyl-CoA
6 NAD+
2 FAD
2 GDP
Intermediate Reactants: Citric Acid
16www.drjayeshpatidar.blogspot.com
ATP production from Carbohydrates
Kreb’s cycle
Products: 2 Oxaloacetic Acid
6 NADH
2 FADH2
2 GTP
4 CO2
17www.drjayeshpatidar.blogspot.com
Overview of the Kreb’s Cycle
18www.drjayeshpatidar.blogspot.com
ATP production from Carbohydrates
Eelectron Transport Chain
A series of Oxidative Phosphorylation reactions
Oxidation = the removal of electrons from a
molecule and results in a decrease in the
energy content of the molecule. Because
most biological reactions involve the loss
of hydrogen atoms, they are called
dehydrogenation reactions.
Reduction = the opposite of oxidation; the
addition of electrons to a molecule, and
results in an increase in the energy
content of the molecule.
19www.drjayeshpatidar.blogspot.com
Overview of Cellular respiration
20www.drjayeshpatidar.blogspot.com
ATP production from Carbohydrates
Electron Transport Chain
Substrates required: 10 NADH
2 FADH
6 O2
Products: 32 ATP
6 H20
21www.drjayeshpatidar.blogspot.com
Overview of Electron Transport System
22www.drjayeshpatidar.blogspot.com
Summary of ATP Production
23www.drjayeshpatidar.blogspot.com
Protein Metabolism
Deamination: removal of the amino group
(NH2) leaving a acetyl molecule
Transamination: Adding an animo group to
pyruvic acid to produce any of the
nonessential amino acids
Protein Synthesis: Production of protein
molecule using an RNA model.
Protein catabolism: breaking down a
protein into individual Amino Acids.
24www.drjayeshpatidar.blogspot.com
Lipid Metabolism
• Lipoproteins: surrounding triglycerides with
apoproteins to make them more transportable
in water
– Very low-density lipoproteins (VLDL’s)
– Low-density lipoproteins (LDL’s)
– High-density lipoprteins (HDL’s)
Lipolysis: break down lipids
Beta Oxidation: breaking a fatty acid into 2
carbon compounds
Lipogenesis: formation of lipids from non-
lipids
25www.drjayeshpatidar.blogspot.com
Overview of the Role of the Liver in Lipid
Metabolism
26www.drjayeshpatidar.blogspot.com
Summary of the Interconnection Between
Tissues and Metabolistic Reactions
27www.drjayeshpatidar.blogspot.com
Metabolic Overview: The Absorptive
State
28www.drjayeshpatidar.blogspot.com
Metabolic overview: the Postabsorptive
State
29www.drjayeshpatidar.blogspot.com
Thank you
www.drjayeshpatidar.blogspot.com 30

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Metabolism

  • 2. Overview of Energy and Metabolism 1. The food we eat, (carbohydrates, lipids, proteins, and Nucleic Acids), are our only source of energy for doing the biological work of cells. 2. All molecules (nutrient molecules included) have stored (potential) energy in the bonds between their atoms. 3. The energy the runs most biological Systems on earth comes from solar energy 4. Plants trap solar energy via the metabolic reactions of Photosynthesis by producing these molecules 2www.drjayeshpatidar.blogspot.com
  • 3. Overview of Energy and Metabolism Terms Calorie: basic unit of measurement of energy in biological system. Indicated by a “c” In human metabolism Calorie is really kilocalorie or 1,000 calories. Indicated by a “C” This calorie “C” is the amount of energy required to raise the temperature to 1 kg of water 1 degree C. The Caloric needs of most organisms is measured as Basal Metabolic Rate (BMR) 3www.drjayeshpatidar.blogspot.com
  • 4. Overview of Energy and Metabolism Terms BMR is the minimum resting energy expenditures by an awake alert person Average BMR: 70 C/hr or 1680 C/day If we assume that average amounts of carbohydrates, lipid, and proteins are being catabolized we can calculate a ratio of 4.825 “C” per liter of O2 consumed. BMR in influence by age, gender, physical condition, body weight, and genes. 4www.drjayeshpatidar.blogspot.com
  • 5. Three Basic Uses of Nutrients Absorbed by the Digestive System 1. Energy for immediate use by cells to conduct their normal Metabolic processes (Muscle contraction, secretions, active transport) 2. Synthesize structural or functional molecules to repair and replace cells. (Mitosis and Cytokinesis) 3. Storage as glycogen or fat for later use as energy (nutrient reserves) 5www.drjayeshpatidar.blogspot.com
  • 6. ALL Living Things from Bacteria to Humans Conduct METABOLISM Metabolism is the ability to acquire and use energy from the environment. Metabolic processes are all the chemical reactions that occur in cells, tissues, organs, and organ systems. Two Kinds of Metabolic Reactions: 1. Catabolism = breakdown of large molecules into simple ones to produce energy. (release energy) 2. Anabolism = build large molecules from simple molecules. (requires energy input) 6www.drjayeshpatidar.blogspot.com
  • 7. Two Basic Kinds of Chemical Reactions found in Biological System 7www.drjayeshpatidar.blogspot.com
  • 8. Metabolic Turnover and Cellular ATP Production 8www.drjayeshpatidar.blogspot.com
  • 9. Carbohydrate Metabolism Amino acid synthesis: Convert pyruvic Acid into an Amino Acid requires a transamination Transamination: Pyruvic Acid + NH3 Amino Acid Glycogenesis: glucose glycogen Lipogenesis: glucose Triglyceride Glycogenolysis: glycogen glucose Gluconeogenesis: lactic acid, amino acids, or triglycerides glucose 9www.drjayeshpatidar.blogspot.com
  • 10. Summary of Glucose Metabolism 10www.drjayeshpatidar.blogspot.com
  • 12. ATP production from Carbohydrates Glycolysis Substrates required: Glucose, 2 ATP, 4 ADP, and 2 NAD+ Intermediate Reactants: Glucose-6-phosphate, Fructose-1,6 bisphosphate Products: 2 molecules of Pyruvic Acid 2 ATP 2 NADH 12www.drjayeshpatidar.blogspot.com
  • 13. Overview of Cellular respiration 13www.drjayeshpatidar.blogspot.com
  • 14. ATP production from Carbohydrates Formation of Acetyl-CoA Substrates required: 2 Pyruvic Acid 2 NAD+ 2 Coenzyme A Products: 2 Acetyl-CoA 2 NADH 2 CO2 14www.drjayeshpatidar.blogspot.com
  • 15. Overview of Cellular respiration 15www.drjayeshpatidar.blogspot.com
  • 16. ATP production from Carbohydrates Kreb’s cycle Substrates required: 2 Oxaloacetic Acid 2 Acetyl-CoA 6 NAD+ 2 FAD 2 GDP Intermediate Reactants: Citric Acid 16www.drjayeshpatidar.blogspot.com
  • 17. ATP production from Carbohydrates Kreb’s cycle Products: 2 Oxaloacetic Acid 6 NADH 2 FADH2 2 GTP 4 CO2 17www.drjayeshpatidar.blogspot.com
  • 18. Overview of the Kreb’s Cycle 18www.drjayeshpatidar.blogspot.com
  • 19. ATP production from Carbohydrates Eelectron Transport Chain A series of Oxidative Phosphorylation reactions Oxidation = the removal of electrons from a molecule and results in a decrease in the energy content of the molecule. Because most biological reactions involve the loss of hydrogen atoms, they are called dehydrogenation reactions. Reduction = the opposite of oxidation; the addition of electrons to a molecule, and results in an increase in the energy content of the molecule. 19www.drjayeshpatidar.blogspot.com
  • 20. Overview of Cellular respiration 20www.drjayeshpatidar.blogspot.com
  • 21. ATP production from Carbohydrates Electron Transport Chain Substrates required: 10 NADH 2 FADH 6 O2 Products: 32 ATP 6 H20 21www.drjayeshpatidar.blogspot.com
  • 22. Overview of Electron Transport System 22www.drjayeshpatidar.blogspot.com
  • 23. Summary of ATP Production 23www.drjayeshpatidar.blogspot.com
  • 24. Protein Metabolism Deamination: removal of the amino group (NH2) leaving a acetyl molecule Transamination: Adding an animo group to pyruvic acid to produce any of the nonessential amino acids Protein Synthesis: Production of protein molecule using an RNA model. Protein catabolism: breaking down a protein into individual Amino Acids. 24www.drjayeshpatidar.blogspot.com
  • 25. Lipid Metabolism • Lipoproteins: surrounding triglycerides with apoproteins to make them more transportable in water – Very low-density lipoproteins (VLDL’s) – Low-density lipoproteins (LDL’s) – High-density lipoprteins (HDL’s) Lipolysis: break down lipids Beta Oxidation: breaking a fatty acid into 2 carbon compounds Lipogenesis: formation of lipids from non- lipids 25www.drjayeshpatidar.blogspot.com
  • 26. Overview of the Role of the Liver in Lipid Metabolism 26www.drjayeshpatidar.blogspot.com
  • 27. Summary of the Interconnection Between Tissues and Metabolistic Reactions 27www.drjayeshpatidar.blogspot.com
  • 28. Metabolic Overview: The Absorptive State 28www.drjayeshpatidar.blogspot.com
  • 29. Metabolic overview: the Postabsorptive State 29www.drjayeshpatidar.blogspot.com