Glycolysis is a central pathway for glucose catabolism that converts glucose into pyruvate through a series of 10 enzyme-catalyzed reactions. It occurs in most organisms and tissues as a source of energy. The first phase activates glucose through phosphorylation, while the second phase generates ATP and NADH through substrate-level phosphorylation and hydride transfer. Pyruvate produced can then undergo aerobic or anaerobic fates including fermentation to regenerate NAD+ under anaerobic conditions.
carbohydrate metabolism, Glycolysis, metabolic process of carbohydrates, EMP ...RajkumarKumawat11
carbohydrate metabolism, Glycolysis, metabolic process of carbohydrates, EMP pathway, Embden- Meyerof-Paranas pathway, cabohydrate metabolic process for study, A presentation on cabohydrate metabolic process i.e. Glycolysis
Chapters 18 - Amino acid Oxidation , production of urea Biochemistry Areej Abu Hanieh
Chapters 18 - Amino acid Oxidation , production of urea Biochemistry
the link for chapter 22 : https://www.slideshare.net/arijabuhaniyeh/chapters-18-22-biochemistry-74720233
carbohydrate metabolism, Glycolysis, metabolic process of carbohydrates, EMP ...RajkumarKumawat11
carbohydrate metabolism, Glycolysis, metabolic process of carbohydrates, EMP pathway, Embden- Meyerof-Paranas pathway, cabohydrate metabolic process for study, A presentation on cabohydrate metabolic process i.e. Glycolysis
Chapters 18 - Amino acid Oxidation , production of urea Biochemistry Areej Abu Hanieh
Chapters 18 - Amino acid Oxidation , production of urea Biochemistry
the link for chapter 22 : https://www.slideshare.net/arijabuhaniyeh/chapters-18-22-biochemistry-74720233
Formation and fate of Ammonia
Transdeamination, oxidative and non oxidative deamination, Ammonia transport, Ammonia intoxication, Ammonia detoxification
PART I INTRODUCTION TO THE CELL 1
Chapter 1 Cells and Genomes 1
Chapter 2 Cell Chemistry and Bioenergetics 43
Chapter 3 Proteins 109
PART II BASIC GENETIC MECHANISMS 173
Chapter 4 DNA, Chromosomes, and Genomes 173
Chapter 5 DNA Replication, Repair, and Recombination 237
Chapter 6 How Cells Read the Genome: From DNA to Protein 299
Chapter 7 Control of Gene Expression 369
PART III WAYS OF WORKING WITH CELLS 439
Chapter 8 Analyzing Cells, Molecules, and Systems 439
Chapter 9 Visualizing Cells 529
PART IV INTERNAL ORGANIZATION OF THE CELL 565
Chapter 10 Membrane Structure 565
Chapter 11 Membrane Transport of Small Molecules and the Electrical
Properties of Membranes 597
Chapter 12 Intracellular Compartments and Protein Sorting 641
Chapter 13 Intracellular Membrane Traffic 695
Chapter 14 Energy Conversion: Mitochondria and Chloroplasts 753
Chapter 15 Cell Signaling 813
Chapter 16 The Cytoskeleton 889
Chapter 17 The Cell Cycle 963
Chapter 18 Cell Death 1021
PART V CELLS IN THEIR SOCIAL CONTEXT 1035
Chapter 19 Cell Junctions and the Extracellular Matrix 1035
Chapter 20 Cancer 1091
Chapter 21 Development of Multicellular Organisms 1145
Chapter 22 Stem Cells and Tissue Renewal 1217
Chapter 23 Pathogens and Infection 1263
Chapter 24 The Innate and Adaptive Immune Systems 1297
Glossary G: 1
Index I: 1
Tables The Genetic Code, Amino Acids T: 1
Metabolic Fate of Pyruvate and Cori cycle and Alanine cycle Cori & Alanine cy...Amany Elsayed
Metabolic Fate of Pyruvate and Cori cycle and Alanine cycle Cori & Alanine cycle and Lactate Dehydrogenase Deficiency (LDHA) and Malate aspartate shuttle (cycle) and Glycerol phosphate shuttle and Mitochondrial shuttle
Carbohydrate metabolism denotes the various biochemical processes responsible for the formation, breakdown and interconversion of carbohydrates in living organisms. The most important carbohydrate is glucose, a simple sugar (monosaccharide) that is metabolized by nearly all known organisms.
Supplying a huge array of metabolic intermediates for biosynthetic reactions. Normally carbohydrate metabolism supplies more than half of the energy requirements of the body. In fact the brain largely depends upon carbohydrate
Carbohydrate metabolism comprises glycolysis, HMP shunt, Gluconeogenesis, Glycogenolysis, TCA cycle, with Glucose-6-phosphate dehydrogenase deficiency disorder.
Announcement about my previous presentations - Thank youAreej Abu Hanieh
ANNOUNCEMENT Thank you for all of you, my followers who sent me messages with a lot of love and appreciations, I finally graduated after 6 years of studying in Birzeit University , In doctor of Pharmacy department I hope all of you benefited from all the presentations posted before Thank you a new PharmD GraduatedAreej ^^
Observation of Io’s Resurfacing via Plume Deposition Using Ground-based Adapt...Sérgio Sacani
Since volcanic activity was first discovered on Io from Voyager images in 1979, changes
on Io’s surface have been monitored from both spacecraft and ground-based telescopes.
Here, we present the highest spatial resolution images of Io ever obtained from a groundbased telescope. These images, acquired by the SHARK-VIS instrument on the Large
Binocular Telescope, show evidence of a major resurfacing event on Io’s trailing hemisphere. When compared to the most recent spacecraft images, the SHARK-VIS images
show that a plume deposit from a powerful eruption at Pillan Patera has covered part
of the long-lived Pele plume deposit. Although this type of resurfacing event may be common on Io, few have been detected due to the rarity of spacecraft visits and the previously low spatial resolution available from Earth-based telescopes. The SHARK-VIS instrument ushers in a new era of high resolution imaging of Io’s surface using adaptive
optics at visible wavelengths.
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Comparing Evolved Extractive Text Summary Scores of Bidirectional Encoder Rep...University of Maribor
Slides from:
11th International Conference on Electrical, Electronics and Computer Engineering (IcETRAN), Niš, 3-6 June 2024
Track: Artificial Intelligence
https://www.etran.rs/2024/en/home-english/
Toxic effects of heavy metals : Lead and Arsenicsanjana502982
Heavy metals are naturally occuring metallic chemical elements that have relatively high density, and are toxic at even low concentrations. All toxic metals are termed as heavy metals irrespective of their atomic mass and density, eg. arsenic, lead, mercury, cadmium, thallium, chromium, etc.
2. Central
Importance
of
Glucose
• Glucose
is
an
excellent
fuel
– Yields
good
amount
of
energy
upon
oxida8on
(ΔGcomplete
oxida8on
=
–2840
kJ/mol)
– Can
be
efficiently
stored
in
the
polymeric
form
– Many
organisms
and
8ssues
can
meet
their
energy
needs
on
glucose
only
• Glucose
is
a
versa8le
biochemical
precursor
– Bacteria
can
use
glucose
to
build
the
carbon
skeletons
of:
• All
the
amino
acids
• Membrane
lipids
• Nucleo8des
in
DNA
and
RNA
• Cofactors
needed
for
the
metabolism
3. Four
Major
Pathways
of
Glucose
U-liza-on
• Storage
– Can
be
stored
in
the
polymeric
form
(starch,
glycogen)
– When
there’s
excess
energy
• Glycolysis
– Generates
energy
via
oxida8on
of
glucose
– Short-‐term
energy
needs
• Pentose
Phosphate
Pathway
– Generates
NADPH
via
oxida8on
of
glucose
– For
detoxifica8on
and
the
biosynthesis
of
lipids
and
nucleo8des
• Synthesis
of
Structural
Polysaccharides
– For
example,
in
cell
walls
of
bacteria,
fungi,
and
plants
4. Glycolysis:
Importance
• Almost
universal
central
pathway
of
glucose
catabolism
• Sequence
of
enzyme-‐catalyzed
reac8ons
by
which
glucose
is
converted
into
pyruvate
• Pyruvate
can
be
further
aerobically
oxidized
• Pyruvate
can
be
used
as
a
precursor
in
biosynthesis
• Some
of
the
oxida8on-‐free
energy
is
captured
by
the
synthesis
of
ATP
and
NADH
• Research
of
glycolysis
played
a
large
role
in
the
development
of
modern
biochemistry
– Understanding
the
role
of
coenzymes
– Discovery
of
the
pivotal
role
of
ATP
– Development
of
methods
for
enzyme
purifica8on
– Inspira8on
for
the
next
genera8ons
of
biochemists
5. The
2
phases
of
glycolysis
• In
the
evolu8on
of
life,
glycolysis
probably
was
one
of
the
earliest
energy-‐yielding
pathways
• It
developed
before
photosynthesis,
when
the
atmosphere
was
s8ll
anaerobic
• Thus,
the
task
upon
early
organisms
was:
How
to
extract
free
energy
from
glucose
anaerobically?
• The
solu8on:
– First:
Ac8vate
it
by
phosphoryla8on
– Second:
Collect
energy
from
the
high-‐energy
metabolites
• Glycolysis
is
a
sequence
of
10
reac8ons,
5
are
preparatory
and
5
are
energy-‐yielding
6. Glycolysis:
The
Preparatory
Phase
For each
molecule of
glucose that
passes through
the preparatory
phase, two
molecules of
glyceraldehyde 3-
phosphate are
formed.
The
“lysis”
step of
glycolysis
2 ATP molecules are
used to raise the free
energy of the
intermediates
7. Glycolysis:
The
Payoff
Phase
4 ATP are produced per glucose
2 ATP/glucose is the net outcome
Energy is also conserved by the
formation of 2 NADH
molecules
3 types of chemical
transformations:
1. Breakage of glucose
backbone to yield pyruvate
(6C 2x 3C)
2. Formation of NADH by hydride
transfer to NAD+
3. Phosphorylation of ADP
by high phosphoryl group
potential compounds to make ATP
8. Chemical
Logic
of
Glycolysis
• How is the formation of NADH and ATP
coupled to glycolysis? (Free energy changes)
• Why are phosphorylated intermediates
important in glycolysis?
9. Glycolysis:
Fates
of
Pyruvate
• In most organisms pyruvate is metabolized via one of three catabolic
routes:
1. Citric acid cycle: pyruvate is oxidized and decarboxylated to release
CO2 (the electrons that are moving go through ETC in mito and are
used to make ATP; aerobic conditions)
2. Lactic acid fermentation: after vigorous exercise, [O2] in muscles is
low (hypoxia) NADH cannot be reoxidized to NAD+ for glycolysis
to continue pyruvate is reduced to lactate accepting electrons
from NADH (regenerating NAD+). Certain tissues (RBC and retina)
ferment pyruvate into lactate even under aerobic conditions
3. Alcohol fermentation: some yeasts and plants can ferment pyruvate
into ethanol and CO2 (important for beverage production and
baking)
• Pyruvate also some anabolic fates (can produce a.a. alanine or fatty
acids)
11. Anaerobic
Glycolysis:
Fermenta-on
• Genera&on
of
energy
(ATP)
without
consuming
oxygen
or
NAD+
• No
net
change
in
oxida8on
state
of
the
sugars
• Reduc8on
of
pyruvate
to
another
product
• Regenerates
NAD+
for
further
glycolysis
under
anaerobic
condi8ons
• The
process
is
used
in
the
produc8on
of
food
from
beer
to
yogurt
to
soy
sauce
12. Yeast
undergo
Ethanol
Fermenta-on
• Two-‐step
reduc8on
of
pyruvate
to
ethanol,
irreversible
• Humans
do
not
have
pyruvate
decarboxylase
• Humans
do
express
alcohol
dehydrogenase
for
ethanol
metabolism
• CO2
produced
in
the
first
step
is
responsible
for:
– carbona8on
in
beer
– dough
rising
when
baking
bread
• Both
steps
require
cofactors
– Pyruvate
decarboxylase:
Mg2+
and
thiamine
pyrophosphate
(TPP)
– Alcohol
dehydrogenase:
Zn2+
and
NADH
13. Animals
undergo
lac-c
acid
fermenta-on
• Reduc8on
of
pyruvate
to
lactate,
reversible
• Equilibrium
favors
lactate
forma8on
• During
strenuous
exercise,
lactate
builds
up
in
the
muscle
– Generally
less
than
1
minute
(even
most
toned
athletes
cannot
sprint
at
highest
speeds
for
more
than
a
minute!)
• The
acidifica8on
of
muscle
prevents
its
con8nuous
strenuous
work
14. Lac-c
Acid
Fermenta-on
The Cori Cycle
• No
net
change
in
NAD+
or
NADH
levels
• Lactate
can
be
transported
to
the
liver
to
be
converted
to
glucose
(the
Cori
cycle)
• Requires
a
recovery
8me
– High
amount
of
oxygen
consump8on
to
fuel
gluconeogenesis
– Restores
muscle
glycogen
stores
– Heavy
breathing
is
required
to
replenish
oxygen
to
repay
the
“oxygen
debt”
15. TPP
is
a
common
acetaldehyde
carrier
• Coenzyme
derived
from
vitamin
B1
(thiamine)
• Lack
of
B1
beriberi
(swelling,
pain,
paralysis
and
death)
• Cleavage
of
bonds
adjacent
to
carbonyl
groups
• Thiazolium
ring
of
TPP
stabilizes
carbanion
intermediates
by
providing
an
electrophilic
structure
into
which
the
carbanion
electrons
can
be
delocalized
by
resonance
“electron
sinks”
acidic proton
16.
17.
The
Preparatory
Phase
18. Step
1:
Phosphoryla-on
of
Glucose
• Ra8onale
– Traps
glucose
inside
the
cell
– Lowers
intracellular
glucose
concentra8on
to
allow
further
uptake
• This
process
uses
the
energy
of
ATP
• The
first
“priming”
reac8on
• Hexokinase
in
eukaryotes,
and
glucokinase
in
prokaryotes
and
liver
(isozymes:
2
or
more
enzymes
encoded
in
different
genes
but
catalyze
the
same
reac8on)
• Soluble
cytosolic
enzyme
(like
all
other
glycoly8c
enzymes)
• Nucleophilic
oxygen
at
C6
of
glucose
alacks
the
last
(γ)
phosphate
of
ATP
• ATP-‐bound
Mg2+
facilitates
this
process
by
shielding
the
nega8ve
charges
on
ATP
• Highly
thermodynamically
favorable/irreversible
– Regulated
mainly
by
substrate
inhibi8on
19. Step
2:
Phosphohexose
Isomeriza-on
• Ra8onale
– C1
of
fructose
is
easier
to
phospho-‐
rylate
by
PFK
– Allows
for
symmetrical
cleave
by
aldolase
• An
aldose
(glucose)
can
isomerize
into
a
ketose
(fructose)
via
an
enediol
intermediate
• The
isomeriza8on
is
catalyzed
by
the
ac8ve-‐site
glutamate,
via
general
acid/base
catalysis
• Slightly
thermodynamically
unfavorable/reversible
– Very
small
posi8ve
ΔG’o
indicates
that
the
reac8on
can
proceed
readily
in
either
direc8on
– Product
concentra8on
kept
low
to
drive
forward
21. Step
3:
2nd
Priming
Phosphoryla-on
• Ra8onale
– Further
ac8va8on
of
glc
– Allows
for
1
phosphate/
3-‐carbon
sugar
aper
step
4
• First
Commiled
Step
of
Glycolysis
– fructose
1,6-‐bisphosphate
is
commiled
to
become
pyruvate
and
yield
energy
whereas
g-‐6-‐p
and
f-‐6-‐p
have
other
possible
fates
• This
process
uses
the
energy
of
ATP
• Highly
thermodynamically
favorable/irreversible
• Phosphofructokinase-‐1
is
highly
regulated
– By
ATP,
ADP,
AMP,
fructose-‐2,6-‐bisphosphate,
and
other
metabolites
(detailed
next
chapter)
– Do
not
burn
glucose
if
there
is
plenty
of
ATP
22. Step
4:
Aldol
Cleavage
of
F-‐1,6-‐bP
• Ra8onale
– Cleavage
of
a
6-‐C
sugar
into
two
3-‐C
sugars
– High-‐energy
phosphate
sugars
are
3-‐C
sugars
• The
reverse
process
is
the
familiar
aldol
condensa8on
• Animal
and
plant
aldolases
employ
covalent
catalysis
• Fungal
and
bacterial
aldolases
employ
metal
ion
catalysis
• Thermodynamically
unfavorable/reversible
– The
actual
free
energy
change
is
small
and
therefore
the
reac8on
is
readily
reversible.
It
is
small
because
the
concentra8on
of
the
reactant
is
kept
low
– GAP
concentra8on
kept
low
to
pull
reac8on
forward
• What
is
the
mechanism
of
aldolase
(class
I)?
23. Step
5:
Triose
Phosphate
Interconversion
• Ra8onale:
– Allows
glycolysis
to
proceed
by
one
pathway
• Aldolase
creates
two
triose
phosphates:
– Dihydroxyacetone
Phosphate
(DHAP)
– Glyceraldehyde-‐3-‐Phosphate
(GAP)
• Only
GAP
is
the
substrate
for
the
next
enzyme
• DHAP
must
be
converted
to
GAP
• Similar
mechanism
as
phosphohexose
isomerase
• Completes
preparatory
phase
• Thermodynamically
unfavorable/reversible
– GAP
concentra8on
kept
low
to
pull
reac8on
forward
24.
The
Payoff
Phase
25. Step
6:
Oxida-on
of
GAP
• Ra8onale:
– Genera8on
of
a
high-‐
energy
phosphate
cpd
– Incorporates
inorganic
phosphate
– Which
allows
for
net
produc-on
of
ATP
via
glycolysis!
• First
energy-‐yielding
step
in
glycolysis
• Oxida8on
of
aldehyde
with
NAD+
gives
NADH
and
an
acyl
phosphate
(very
high
ΔG’o
=
–
49.3
kJ/mol)
• Ac8ve
site
cysteine
– Forms
high-‐energy
thioester
intermediate
– Subject
to
inac8va8on
by
oxida8ve
stress
• Thermodynamically
unfavorable/reversible
– Coupled
to
next
reac8on
to
pull
forward
• GAPDH
mechanism
(self
study)
26. Step
7:
1st
Produc-on
of
ATP
• Ra8onale:
– Substrate-‐level
phosphoryla8on
to
make
ATP
• 1,3-‐bisphosphoglycerate
is
a
high-‐energy
compound
–
can
donate
the
phosphate
group
to
ADP
to
make
ATP
• The
enzyme
is
named
aper
the
reverse
reac8on
• Substrate-‐level
phosphoryla-on:
the
fprma8on
of
ATP
by
group
transfer
from
a
substrate
• Highly
thermodynamically
favorable/reversible
– Is
reversible
because
of
coupling
to
GAPDH
reac8on
– Steps
6
and
7
are
strongly
coupled:
Glyceraldehyde
3-‐P
+
ADP
+
Pi
+
NAD+
3-‐phosphoglycerate
+
ATP
+
NADH
+
H+
ΔG’o
=
–12.2
kJ/mol
27. Step
8:
Migra-on
of
the
Phosphate
• Ra8onale:
– Be
able
to
form
high-‐energy
phosphate
compound
• Mutases
catalyze
the
(apparent)
migra8on
of
func8onal
groups
• One
of
the
ac8ve
site
his8dines
is
post-‐transla8onally
modified
to
phosphohis8dine
• Phosphohis8dine
donates
its
phosphate
to
O
at
C2
before
retrieving
another
phosphate
from
O
at
C3
• 2,3-‐bisphosphoglycerate
intermediate
• Note
that
the
phosphate
from
the
substrate
ends
up
bound
to
the
enzyme
at
the
end
of
the
reac8on
• Thermodynamically
unfavorable/reversible
• Reactant
concentra8on
kept
high
by
PGK
to
push
forward
28. Step
9:
Dehydra-on
of
2-‐PG
to
PEP
• Ra8onale
– Generate
a
high-‐energy
phosphate
compound
• 2-‐Phosphoglycerate
is
not
a
good
enough
phosphate
donor
(ΔG’o
=
–17.6
kJ/mol;
ΔG’o
PEP
=
–61.9
kJ/mol)
• Slightly
thermodynamically
unfavorable/reversible
• Product
concentra8on
kept
low
to
pull
forward
29. Step
10:
2nd
Produc-on
of
ATP
• Ra8onale
– Substrate-‐level
phosphoryla8on
to
make
ATP
– Net
produc8on
of
2
ATP/
glucose
• Loss
of
phosphate
from
PEP
yields
an
enol
that
tautomerizes
into
ketone
• Tautomeriza8on
• effec8vely
lowers
the
concentra8on
of
the
reac8on
product
• drives
the
reac8on
toward
ATP
forma8on
• Pyruvate
kinase
requires
divalent
metals
(Mg2+
or
Mn2+)
for
ac8vity
• Highly
thermodynamically
favorable/irreversible
• Regulated
by
ATP,
divalent
metals,
and
other
metabolites
31. Summary
of
Glycolysis
• Used:
– 1
glucose;
2
ATP;
2
NAD+
• Made:
– 2
pyruvate
• Various
different
fates
– 4
ATP
• Used
for
energy-‐requiring
processes
within
the
cell
– 2
NADH
• Must
be
reoxidized
to
NAD+
in
order
for
glycolysis
to
con8nue
• Glycolysis
is
heavily
regulated
– Ensure
proper
use
of
nutrients
– Ensure
produc8on
of
ATP
only
when
needed
– Under
anaerobic
condi8ons,
both
the
rate
and
the
total
amount
of
glucose
consump8on
are
many
8mes
greater
than
with
oxygen
present,
why???
Glucose + 2 NAD+ + 2 ADP + 2 Pi à 2 Pyruvate + 2 NADH + 2 H+ + 2 H2O+ 2 ATP
32.
33. Glycolysis
occurs
at
elevated
rates
in
tumor
cells
• Warburg
effect:
tumor
cells
carry
out
glycolysis
at
a
much
higher
rate
than
normal
cells
even
when
oxygen
is
available
(~10x)
• In
general,
the
more
aggressive
the
tumor,
the
greater
is
its
rate
of
glycolysis
• HIF-‐1
(hypoxia-‐inducible
transcrip8on
factor)
s8mulates
the
produc8on
of
at
least
8
glycoly8c
enzymes
and
glucose
transporters
when
the
oxygen
supply
is
limited
• HIF-‐1
also
s8mulates
the
produc8on
of
VEGF
(which
s8mulates
angiogenesis)
• Overreliance
of
tumors
on
glycolysis
suggests
a
possibility
for
an8cancer
therapy:
deplete
ATP
from
cancer
cells
by
blocking
glycolysis
• PET
scans
take
advantage
of
the
high
uptake
of
glucose
by
tumor
cells.
Used
to
pinpoint
cancers
34. Glucose
uptake
is
deficient
in
type
1
Diabetes
Mellitus
• Glucose
uptake
into
cells
is
mediated
by
GLUT
family
• GLUT1
&
GLUT2
(hepatocytes)
and
GLUT3
(brain
neurons)
are
always
present
in
the
plasma
membrane
of
these
cells
• GLUT4
(skeletal
and
cardiac
muscles
and
adipose)
only
move
to
the
plasma
membrane
in
response
to
an
insulin
signal
• Pa8ents
with
type
1
DM
have
too
few
β
cells
in
the
pancreas
(cannot
synthesize
enough
insulin)
heart,
muscles
and
fat
8ssues
cannot
uptake
glucose
hyperglycemia
(aper
carb-‐rich
meals)
• Fat
cells
turn
to
fat
metabolism
to
provide
alterna8ve
energy
forma8on
of
ketone
bodies
• In
untreated
type
1
DM
ketoacidosis
is
common
and
is
life-‐
threatening
• Reversed
by
insulin
injec8on
36. Feeder
Pathways
for
Glycolysis
• Glucose
molecules
are
cleaved
from
endogenous
glycogen
by
glycogen
phosphorylase
(phosphorolysis)
– Yielding
glucose-‐1-‐phosphate
• Dietary
starch
and
glycogen
are
cleaved
by
α-‐amylase
to
produce
oligosaccharides
and
subsequently
maltose
and
maltotriose
in
the
small
intes8ne,
by
pancrea8c
α-‐
amylase
(hydrolysis)
• Disaccharides
are
hydrolyzed
– Lactose:
glucose
and
galactose
(lactose
intolerance?)
– Sucrose:
glucose
and
fructose
– Fructose,
galactose,
and
mannose
enter
glycolysis
at
different
points
37. Gluconeogenesis:
Precursor
for
Carbohydrates
No&ce
that
mammals
cannot
convert
faIy
acids
to
sugars.
• Brain
and
nerve
cells,
RBC,
renal
medulla,
testes
an
embryonic
8ssue
use
only
glucose
as
the
energy
source
-‐
120
g
of
glucose
daily
(brain)
• Synthesizing
glucose
from
noncarbohydrate
precursors
–
gluconeogenesis
• In
mammals,
occurs
in
the
liver
(mainly)
and
in
renal
cortex
38. Glycolysis
vs.
Gluconeogenesis
Gluconeogenesis
occurs
mainly
in
the
liver.
Glycolysis
occurs
mainly
in
the
muscle
and
brain.
• Not
iden8cal
pathways
running
in
opposite
direc8ons
• 7
of
the
10
reac8ons
of
gluconeogenesis
are
the
reverse
of
glycolysis
• Both
are
irreversible
in
cells
• Both
occur
in
the
cytosol
(reciprocal
and
coordinated
regula8on)
• Opposing
pathways
that
are
both
thermodynamically
favorable
– Operate
in
opposite
direc8on
•
end
product
of
one
is
the
star8ng
cpd
of
the
other
• Reversible
reac8ons
are
used
by
both
pathways
• Irreversible
reac8on
of
glycolysis
must
be
bypassed
in
gluconeogenesis
– Highly
thermodynamically
favorable,
and
regulated
– Different
enzymes
in
the
different
pathways
– Differen8ally
regulated
to
prevent
a
fu8le
cycle
39. Pyruvate
to
Phosphoenolpyruvate
• Requires
two
energy-‐consuming
steps
• First
step,
pyruvate
carboxylase
converts
pyruvate
to
oxaloacetate
– Carboxyla8on
using
a
bio8n
cofactor
– Requires
transport
into
mitochondria
– First
regulatory
enzyme
in
gluconeogenesis
(acetyl
CoA
is
+ve
effector)
• Second
step,
phosphoenolpyruvate
carboxykinase
converts
oxaloacetate
to
PEP
– Phosphoryla8on
from
GTP
and
decarboxyla8on
– Occurs
in
mitochondria
or
cytosol
depending
on
the
organism
Carboxylation-decarboxylation
sequences activate pyruvate
40. Bio-n
is
a
CO2
Carrier
• Bio8n
is
covalently
alached
to
the
enzyme
through
an
amide
linkage
to
the
ε-‐amino
group
of
a
Lys
residue
• The
reac8on
occurs
in
two
phases
(at
two
different
sites):
• At
cataly8c
site
1,
bicarbonate
ion
is
converted
to
CO2
at
the
expense
of
ATP.
CO2
reacts
with
bio8n,
forming
carboxybio8nyl-‐enzyme
• The
long
arm
carries
the
CO2
of
carboxybio8nylenzyme
to
cataly8c
site
2
on
the
enzyme
surface,
where
CO2
is
released
and
reacts
with
the
pyruvate,
forming
oxaloacetate
• The
general
role
of
flexible
arms
in
carrying
reac8on
intermediates
between
enzyme
ac8ve
sites
41. Malate
dehydrogenase
• No
transporter
of
oxaloacetate
in
mitochondria
• OA
must
be
reduced
to
malate
by
mitochondrial
malate
dehydrogenase
using
NADH
OA
+
NADH
+
H+
L-‐malate
+
NAD+
• Very
low
[OA]
makes
the
ΔG
~
0
despite
the
high
ΔG’o
• In
cytosol,
L-‐malate
is
reoxidized
producing
NADH
L-‐malate
+
NAD+
OA
+
NADH
+
H+
• [NADH]/[NAD+]mito
>
[NADH]/[NAD+]cyto
105x
cytosolic
NADH
is
consumed
in
gluconeogenesis,
glucose
produc8on
cannot
con8nue
unless
NADH
is
available.
Moving
malate
from
mito
to
cytosol
moves
also
NADH
equivalents
to
allow
the
process
to
occur
42. Overall
bypass
reac-on
• OA
+
GTP
PEP
+
CO2
+
GDP
(PEP
carboxykinase)
• Reversible
under
cellular
condi8ons:
forma8on
of
one
high
energy
phosphate
is
balanced
by
the
hydrolysis
of
another
• Pyruvate
+
ATP
+
GTP
+
HCO3
-‐
PEP
+
CO2
+
ADP
+
GDP
+
Pi ΔG’o
=
0.9
kJ/mol
•
ΔG
for
the
reac8on
~
–25
kJ/mol
because
the
actual
cellular
[PEP]
is
very
low
the
reac8on
is
irreversible
in
vivo
43. Addi-onal
bypasses
• RBC
and
anaerobic
muscle
cells,
lactate
predominates
• Converted
to
pyruvate
by
LDH
• Produces
NADH
in
the
cytosol,
no
need
for
malate
conversion
• OA
is
decarboxylated
by
mito
PEP
carboxykinase
and
PEP
is
exported
from
mito
44. Addi-onal
Bypasses
• Catalyze
reverse
reac8on
of
opposing
step
in
glycolysis
• Are
irreversible
themselves
• Fructose
1,6-‐bisphosphate
Fructose
6-‐Phosphate
– By
fructose
bisphosphatase-‐1
(FBPase-‐1)
– Coordinately/oppositely
regulated
with
PFK
– A
hydrolysis
reac8on
with
ΔG’o
=
–
16.3
kJ/mol
• Glucose
6-‐phosphate
Glucose
– By
glucose
6-‐phosphatase
– A
hydrolysis
reac8on
with
ΔG’o
=
–
13.8
kJ/mol
– Enzyme
found
in
hepatocytes,
renal
medulla
and
intes8nal
epithelial
cells,
NOT
anywhere
else
(if
it
were
found
everywhere,
…
what
do
you
expect
would
happen?)
45. Gluconeogenesis
is
expensive
• Costs
4
ATP,
2
GTP,
and
2
NADH
• Not
the
reversal
of
the
conversion
of
pyr
to
glc
• But
physiologically
necessary
to
ensure
irreversibility
• Also,
there’s
a
need
to
keep
pyruvate
inside
the
cell
instead
of
secre8ng
it
outside.
Pyruvate
has
the
poten8al
to
make
more
than
10
ATP
per
full
oxida8on
of
pyruvate
• Brain,
nervous
system,
and
red
blood
cells
generate
ATP
ONLY
from
glucose
2
Pyruvate
+
4
ATP
+
2
GTP
+
2
NADH
+
2
H+
+
4
H2O
Glucose
+
4
ADP
+
2
GDP
+
6
Pi
+
2
NAD+
46. Precursors
for
Gluconeogenesis
• Glucose
can
be
produced
from
all
intermediates
of
the
CAC
(citrate,
isocitrate,
α-‐KG,
succinyl-‐CoA
,
succinate,
fumarate
and
malate)
since
all
of
them
can
undergo
oxida8on
to
OA
• Also,
most
a.a.
can
undergo
transforma8ons
to
pyruvate
or
CAC
intermediate,
and
therefore
has
the
poten8al
to
make
glucose:
i.e.
glucogenic
-‐
Only
Leu
and
Lys
are
non-‐glucogenic
-‐
Ala
and
Gln
are
par8cularly
important
glucogenic
a.a.
in
mammals
47. Precursors
for
Gluconeogenesis
• Animals
can
produce
glucose
from
sugars
or
proteins
and
parts
of
fat
(triacylglycerol)
– Sugars:
pyruvate,
lactate,
or
oxaloacetate
– Protein:
from
glucogenic
a.a.
– Glycerol:
the
breakdown
product
of
fats
can
be
used
aper
a
two
step
reac8on.
Glycerol
kinase
phosphorylates
it
and
the
oxida8on
of
the
central
C
yields
dihydroxyacetone
phosphate
(an
intermediate
in
gluconeogenesis)
• Animals
cannot
produce
glucose
from
faly
acids
– Product
of
faly
acid
degrada8on
is
acetyl-‐CoA
– Cannot
have
a
net
conversion
of
acetyl-‐CoA
to
oxaloacetate
(2
C
that
enter
the
CAC
are
removed
as
2CO2)
• Plants,
yeast,
and
many
bacteria
can
do
this
(the
glyoxylate
cycle),
thus
producing
glucose
from
faly
acids
48.
49. Pentose
Phosphate
Pathway
• Glc
6-‐P
has
another
catabolic
fate
which
leads
to
specialized
products
needed
by
cells
• The
main
products
are
NADPH
and
ribose
5-‐phosphate
• NADPH
is
an
electron
donor
– Reduc8ve
biosynthesis
of
faly
acids
and
steroids
(liver,
adipose,
gonads,
etc.)
– Repair
of
oxida8ve
damage
esp.
in
cells
directly
exposed
to
O2
(RBC,
cornea)
• Ribose-‐5-‐phosphate
is
a
biosynthe8c
precursor
of
nucleo8des
– Used
in
DNA
and
RNA
synthesis
esp.
in
rapidly
dividing
cells
(skin,
bone
marrow,
tumors,
etc.)
– Or
synthesis
of
some
coenzymes
(ATP,
NADH,
FADH )
51. Oxida-ve
phase
generates
NADPH
and
R-‐5-‐P
1. Oxida8on
of
G-‐6-‐P
to
δ-‐lactone
by
G6PD,
reduc8on
of
NADP+
2. Lactone
hydrolysis
by
lactonase
3. Oxida8on
and
decarboxyla8on
by
6-‐PG
dehydrogenase
to
produce
ribulose
5-‐P
4. Forma8on
of
ribose
5-‐P
by
phosphopentose
isomerase
• Pentose
pathway
ends
here
in
some
8ssues
Essentially
irreversible
52. Non-‐oxida-ve
phase
regenerates
G-‐6-‐P
from
R-‐5-‐P
• Used
in
8ssues
requiring
more
NADPH
than
R-‐5-‐P
(e.g.
liver
and
adipose)
• Six
5-‐C
sugar
phosphates
are
converted
into
five
6-‐C
ones,
allowing
con8nued
G6P
oxida8on
and
NADPH
produc8on
• Details
are
not
important,
but
remember
the
two
key
enzymes
unique
in
this
pathway:
transketolase
and
transaldolase
Wernicke-Korsakoff
syndrome: thiamine
deficiency exacerbated by
transketolase defect
53. Glycolysis,
gluconeogenesis
and
pentose
phosphate
pathway
• All
enzymes
of
PP
are
in
the
cytosol
• Glycolysis,
gluconeogenesis
and
PP
are
connected
through
several
shared
intermediates
and
enzymes:
glc
pyr
Depending on the
cell’s relative needs
for NADPH, ATP and
pentose phosphates
54. NADPH
regulates
par--oning
into
glycolysis
vs.
pentose
phosphate
pathway
G6P can enter glycolysis or PP depending
on the current needs to the cell and the
concentration of NADP+ and NADPH
55. Ques-on
3
(Take
home
exam)
Due:
NEXT
WEEK
(js-ban@birzeit.edu)
• Please
solve
ques-ons:
1. 14
(Arsenate
poisoning)
2. 16
(Niacin)
3. 18
(Clinical
symptoms
of
enzyme
deficiency)
4. 25
(Ethanol
affects
blood
glucose)
5. 28
(Phloridzin)
For
wriIen
answers,
I
prefer
to
have
them
typed
in
Word.
I
can
accept
the
assignment
in
one
file
sent
to
my
email.
For
answers
that
require
solving
mathema&cally,
you
can
either
type
them
or
write
them
down
and
scan
them.