International Journal of Engineering Inventions (IJEI) provides a multidisciplinary passage for researchers, managers, professionals, practitioners and students around the globe to publish high quality, peer-reviewed articles on all theoretical and empirical aspects of Engineering and Science.
Samir Khanal, Professor of Biological Engineering Molecular Biosciences and Bioengineering at UHM, describes an integrated approach in converting biomass into biofuel and biobased products. Slides from the REIS seminar series at the University of Hawaii at Manoa on 2009-10-22.
Miguel G. Guerrero del Instituto de Bioqiímica Vegetal y Fotosíntesis de la Universidad de Sevilla-CSIC, presenta el mercado de producción de Bioethanol de microalgas y las ventajas de usar microalgas a la hora de producir BIoethanol.
8_04_2010
Samir Khanal, Professor of Biological Engineering Molecular Biosciences and Bioengineering at UHM, describes an integrated approach in converting biomass into biofuel and biobased products. Slides from the REIS seminar series at the University of Hawaii at Manoa on 2009-10-22.
Miguel G. Guerrero del Instituto de Bioqiímica Vegetal y Fotosíntesis de la Universidad de Sevilla-CSIC, presenta el mercado de producción de Bioethanol de microalgas y las ventajas de usar microalgas a la hora de producir BIoethanol.
8_04_2010
The world is confronted with twin crisis of fossil fuel depletion and environment degradation. The indiscriminate extraction and consumption of fossil fuels has led to reduction in petroleum reserves. Petroleum based fuels are obtained from limited resources. These finite reserves are highly concentrated in certain region of the word. Therefore, those countries that do not have are facing a foreign exchange crisis, mainly due to the import of crude petroleum oil. Hence it is necessary to look for alternative fuels. Which can be produced from materials available within the country. In the present scenario, agricultural and food waste is increasingly being considered a valuable resource. This way of using that waste reduces the cost of production of bio-ethanol and the problem related to the disposal of waste. Bio-ethanol can be produced using fruit waste by finding it reducing sugar value and by undergoing fermentation process and using some ca talyst respectively in each of the process. Each process must be maintained in pre-determined temperature for the to be the success. The fuel properties namely flash and fire point, kinematic viscosity etc, were studied. It was found that the properties were quite comparable to the properties of the petroleum fuel. By using agricultural waste to produce bio-ethanol, it reduces the cost of production and environmental impact related to the disposal of wastes.
Biodiesel is produced by transesterification of
triglycérides present in animal fat or vegetable oils, by
displacing glycerine with a low molar mass atcobol. This
resulting ester mixture has physico-chemical properties
similar to those of petroleum diesel.
This paper reviews the synthetic paths that lead to
biodiesel by means of the catalytic transesterification of
vegetable oils. Although methyl esters are at present the only
ones produced at industrial scale, the use of ethanol, which
can also be obtained from renewable resources, has been
considered, since it would generate a cleaner and more
biocompatible fuel.
seminar horticulture.
Bioethanol production from fruit and vegetable wastes
The need for energy is continuously increasing due to rapid increase in industrialization and automobiles usage. The major sources to fulfil these energy demands are petroleum, natural gas, coal, hydro and nuclear energy. Increasing concern of fuels as well as escalating social and industrial awareness towards global climate change leads to exploration for the clean renewable fuels (Saifuddin et al., 2014). Therefore, bioethanol production from food sources as well as non-edible feed stocks as a renewable source of energy is believed to be one of the options wide open, to answer our concern towards climate change.
Research is being carried¬-out to convert food waste or inedible parts of fruits like peel and seeds into bioethanol. Although the idea is not new, but has gained considerable attention in recent years due to the escalating price of petro-fuel throughout the world.
Memon et al. (2017) conducted studies on bioethanol production from waste potatoes as a sustainable waste-to-energy resource via enzymatic hydrolysis. The results showed that significant bioethanol production was achieved at 30°C, 6 pH and 84 hours incubation time. About 42 ml of bioethanol was produced from 200 g of potato wastes.
Similarly, Saifuddin et al. (2014) experimented on bioethanol production from mango waste (Mangifera indica L. cv Chokanan). The highest production of bioethanol yield could be obtained from mango pulp of rotten fruits in the 3g/L of yeast concentration at a temperature of 30°C that yielded 15 per cent (v/v) of ethanol. Ethanol production increased with the increase in fermentation time until five days of incubation.
Comparative studies of ethanol production from different fruit wastes using Saccharomyces cerevisiae, revealed that the rate of ethanol production through fermentation of grape fruit waste was very high (6.21%) followed by banana (5.4%), apple (4.73%) and papaya (4.19%) (Janani et al., 2013).
Studies on production of bioethanol using rinds of pineapple, jackfruit, watermelon and muskmelon by saccharification and fermentation process were undertaken by Bhandari et al., (2013). Significant amounts of ethanol was obtained at the end of the process, with jackfruit rind (4.64g/L) followed by pineapple rind (4.38g/L).
Results of the experiment conducted on production of bioethanol from cassava and sweet potato peels revealed that maximum yield was obtained in cassava (26%) and sweet potato (12%) using combination of Gloeophyllum sepiarium and Pleurotus ostreatus for hydrolysis and combination of Zymomonas mobilis and Saccharomyces cerevisiae for fermentation (Oyeleke et al., 2012).
Biodiesel Production from waste Oil with Micro-Scale Biodiesel System Under L...IJERDJOURNAL
ABSTRACT:- The aim of this project is to produce biodiesel from waste oil. The use of vegetable oils as diesel fuel started with the invention of diesel engines in the 1900s and is also common in many countries today. The fact that the oils used in biodiesel production are also an important input of the food industry is a limiting factor in production. For this reason, it is aimed to produce biodiesel from waste oil which can not be assessed in food production in this study. The most important contribution of the study to biodiesel researches is the establishment of a small-capacity biodiesel unit in laboratory conditions. The waste oils from the food production facilities of Namık Kemal University (NKU) have been collected and biodiesel has been produced using two different experimental methods. The analyses that determine the quality of the biodiesel samples have been carried out by Energy Agriculture Research Center of Black Sea Agricultural Research Institute in Republic of Turkey Ministry of Food, Agriculture and Livestock. As a result of the research, it has been determined that the biodiesel fuel obtained by the B-1 method using KOH as a catalyst conforms to the standards and can be used with confidence in diesel engines.
biobutanol is an advanced biofuel, it has better properties than ethanol and gasoline .it can be transported via existing pipelines and can be used in current engines. ethanol plants can be easily converted to biobutanol plants.
Biochemical Conversion of Lignocellulosic Biomass
Conversion in Biorefineries
Thermochemical Conversion: Production of Biofuels via Gasification
Chemical Technologies
The world is confronted with twin crisis of fossil fuel depletion and environment degradation. The indiscriminate extraction and consumption of fossil fuels has led to reduction in petroleum reserves. Petroleum based fuels are obtained from limited resources. These finite reserves are highly concentrated in certain region of the word. Therefore, those countries that do not have are facing a foreign exchange crisis, mainly due to the import of crude petroleum oil. Hence it is necessary to look for alternative fuels. Which can be produced from materials available within the country. In the present scenario, agricultural and food waste is increasingly being considered a valuable resource. This way of using that waste reduces the cost of production of bio-ethanol and the problem related to the disposal of waste. Bio-ethanol can be produced using fruit waste by finding it reducing sugar value and by undergoing fermentation process and using some ca talyst respectively in each of the process. Each process must be maintained in pre-determined temperature for the to be the success. The fuel properties namely flash and fire point, kinematic viscosity etc, were studied. It was found that the properties were quite comparable to the properties of the petroleum fuel. By using agricultural waste to produce bio-ethanol, it reduces the cost of production and environmental impact related to the disposal of wastes.
Biodiesel is produced by transesterification of
triglycérides present in animal fat or vegetable oils, by
displacing glycerine with a low molar mass atcobol. This
resulting ester mixture has physico-chemical properties
similar to those of petroleum diesel.
This paper reviews the synthetic paths that lead to
biodiesel by means of the catalytic transesterification of
vegetable oils. Although methyl esters are at present the only
ones produced at industrial scale, the use of ethanol, which
can also be obtained from renewable resources, has been
considered, since it would generate a cleaner and more
biocompatible fuel.
seminar horticulture.
Bioethanol production from fruit and vegetable wastes
The need for energy is continuously increasing due to rapid increase in industrialization and automobiles usage. The major sources to fulfil these energy demands are petroleum, natural gas, coal, hydro and nuclear energy. Increasing concern of fuels as well as escalating social and industrial awareness towards global climate change leads to exploration for the clean renewable fuels (Saifuddin et al., 2014). Therefore, bioethanol production from food sources as well as non-edible feed stocks as a renewable source of energy is believed to be one of the options wide open, to answer our concern towards climate change.
Research is being carried¬-out to convert food waste or inedible parts of fruits like peel and seeds into bioethanol. Although the idea is not new, but has gained considerable attention in recent years due to the escalating price of petro-fuel throughout the world.
Memon et al. (2017) conducted studies on bioethanol production from waste potatoes as a sustainable waste-to-energy resource via enzymatic hydrolysis. The results showed that significant bioethanol production was achieved at 30°C, 6 pH and 84 hours incubation time. About 42 ml of bioethanol was produced from 200 g of potato wastes.
Similarly, Saifuddin et al. (2014) experimented on bioethanol production from mango waste (Mangifera indica L. cv Chokanan). The highest production of bioethanol yield could be obtained from mango pulp of rotten fruits in the 3g/L of yeast concentration at a temperature of 30°C that yielded 15 per cent (v/v) of ethanol. Ethanol production increased with the increase in fermentation time until five days of incubation.
Comparative studies of ethanol production from different fruit wastes using Saccharomyces cerevisiae, revealed that the rate of ethanol production through fermentation of grape fruit waste was very high (6.21%) followed by banana (5.4%), apple (4.73%) and papaya (4.19%) (Janani et al., 2013).
Studies on production of bioethanol using rinds of pineapple, jackfruit, watermelon and muskmelon by saccharification and fermentation process were undertaken by Bhandari et al., (2013). Significant amounts of ethanol was obtained at the end of the process, with jackfruit rind (4.64g/L) followed by pineapple rind (4.38g/L).
Results of the experiment conducted on production of bioethanol from cassava and sweet potato peels revealed that maximum yield was obtained in cassava (26%) and sweet potato (12%) using combination of Gloeophyllum sepiarium and Pleurotus ostreatus for hydrolysis and combination of Zymomonas mobilis and Saccharomyces cerevisiae for fermentation (Oyeleke et al., 2012).
Biodiesel Production from waste Oil with Micro-Scale Biodiesel System Under L...IJERDJOURNAL
ABSTRACT:- The aim of this project is to produce biodiesel from waste oil. The use of vegetable oils as diesel fuel started with the invention of diesel engines in the 1900s and is also common in many countries today. The fact that the oils used in biodiesel production are also an important input of the food industry is a limiting factor in production. For this reason, it is aimed to produce biodiesel from waste oil which can not be assessed in food production in this study. The most important contribution of the study to biodiesel researches is the establishment of a small-capacity biodiesel unit in laboratory conditions. The waste oils from the food production facilities of Namık Kemal University (NKU) have been collected and biodiesel has been produced using two different experimental methods. The analyses that determine the quality of the biodiesel samples have been carried out by Energy Agriculture Research Center of Black Sea Agricultural Research Institute in Republic of Turkey Ministry of Food, Agriculture and Livestock. As a result of the research, it has been determined that the biodiesel fuel obtained by the B-1 method using KOH as a catalyst conforms to the standards and can be used with confidence in diesel engines.
biobutanol is an advanced biofuel, it has better properties than ethanol and gasoline .it can be transported via existing pipelines and can be used in current engines. ethanol plants can be easily converted to biobutanol plants.
Biochemical Conversion of Lignocellulosic Biomass
Conversion in Biorefineries
Thermochemical Conversion: Production of Biofuels via Gasification
Chemical Technologies
biofuels, first and second generation biofuels, their history, biodiesel, mass production, applications, properties, fuel efficiency, emissions, material compatibility, availability and prices
Comparative Ethanol Productivities of Two Different Recombinant Fermenting St...IJERA Editor
Production of biofuel such as ethanol from lignocellulosic biomass is a beneficial way to meet sustainability and energy security in the future. The main challenge in bioethanol conversion is the high cost of processing, in which enzymatic hydrolysis and fermentation are the major steps. Among the strategies to lower processing costs are utilizing both glucose and xylose sugars present in biomass for conversion. An approach featuring enzymatic hydrolysis and fermentation steps, identified as separate hydrolysis and fermentation (SHF) was used in this work. Proposed solution is to use “pre-processing” technologies, including the thermal screw press (TSP) and cellulose-organic-solvent based lignocellulose fractionation (COSLIF) pretreatments. Such treatments were conducted on a widely available feedstock such as source separated organic waste (SSO) to liberate all sugars to be used in the fermentation process. Enzymatic hydrolysis was featured with addition of commercial available enzyme, Accellerase 1500, to mediate enzymatic hydrolysis process. On average, the sugar yield from the TSP and COSLIF pretreatments followed by enzymatic hydrolysis was remarkable at 90%. In this work, evaluation of the SSO hydrolysate obtained from COSLIF and enzymatic hydrolysis pretreaments on ethanol yields was compared by fermentation results with two different recombinant strains: Zymomonas mobilis 8b and Saccharomyces cerevisiae DA2416. At 48 hours of fermentation, ethanol yield was equivalent to 0.48g of ethanol produced per gram of SSO biomass by Z.mobilis 8b and 0.50g of ethanol produced per gram of SSO biomass by S. cerevisiae DA2416. This study provides important insights for investigation of the source-separated organic (SSO) waste on ethanol production by different strains and becomes a useful tool to facilitate future process optimization for pilot scale facilities.
PHP Frameworks: I want to break free (IPC Berlin 2024)Ralf Eggert
In this presentation, we examine the challenges and limitations of relying too heavily on PHP frameworks in web development. We discuss the history of PHP and its frameworks to understand how this dependence has evolved. The focus will be on providing concrete tips and strategies to reduce reliance on these frameworks, based on real-world examples and practical considerations. The goal is to equip developers with the skills and knowledge to create more flexible and future-proof web applications. We'll explore the importance of maintaining autonomy in a rapidly changing tech landscape and how to make informed decisions in PHP development.
This talk is aimed at encouraging a more independent approach to using PHP frameworks, moving towards a more flexible and future-proof approach to PHP development.
The Art of the Pitch: WordPress Relationships and SalesLaura Byrne
Clients don’t know what they don’t know. What web solutions are right for them? How does WordPress come into the picture? How do you make sure you understand scope and timeline? What do you do if sometime changes?
All these questions and more will be explored as we talk about matching clients’ needs with what your agency offers without pulling teeth or pulling your hair out. Practical tips, and strategies for successful relationship building that leads to closing the deal.
Neuro-symbolic is not enough, we need neuro-*semantic*Frank van Harmelen
Neuro-symbolic (NeSy) AI is on the rise. However, simply machine learning on just any symbolic structure is not sufficient to really harvest the gains of NeSy. These will only be gained when the symbolic structures have an actual semantics. I give an operational definition of semantics as “predictable inference”.
All of this illustrated with link prediction over knowledge graphs, but the argument is general.
Builder.ai Founder Sachin Dev Duggal's Strategic Approach to Create an Innova...Ramesh Iyer
In today's fast-changing business world, Companies that adapt and embrace new ideas often need help to keep up with the competition. However, fostering a culture of innovation takes much work. It takes vision, leadership and willingness to take risks in the right proportion. Sachin Dev Duggal, co-founder of Builder.ai, has perfected the art of this balance, creating a company culture where creativity and growth are nurtured at each stage.
UiPath Test Automation using UiPath Test Suite series, part 4DianaGray10
Welcome to UiPath Test Automation using UiPath Test Suite series part 4. In this session, we will cover Test Manager overview along with SAP heatmap.
The UiPath Test Manager overview with SAP heatmap webinar offers a concise yet comprehensive exploration of the role of a Test Manager within SAP environments, coupled with the utilization of heatmaps for effective testing strategies.
Participants will gain insights into the responsibilities, challenges, and best practices associated with test management in SAP projects. Additionally, the webinar delves into the significance of heatmaps as a visual aid for identifying testing priorities, areas of risk, and resource allocation within SAP landscapes. Through this session, attendees can expect to enhance their understanding of test management principles while learning practical approaches to optimize testing processes in SAP environments using heatmap visualization techniques
What will you get from this session?
1. Insights into SAP testing best practices
2. Heatmap utilization for testing
3. Optimization of testing processes
4. Demo
Topics covered:
Execution from the test manager
Orchestrator execution result
Defect reporting
SAP heatmap example with demo
Speaker:
Deepak Rai, Automation Practice Lead, Boundaryless Group and UiPath MVP
Transcript: Selling digital books in 2024: Insights from industry leaders - T...BookNet Canada
The publishing industry has been selling digital audiobooks and ebooks for over a decade and has found its groove. What’s changed? What has stayed the same? Where do we go from here? Join a group of leading sales peers from across the industry for a conversation about the lessons learned since the popularization of digital books, best practices, digital book supply chain management, and more.
Link to video recording: https://bnctechforum.ca/sessions/selling-digital-books-in-2024-insights-from-industry-leaders/
Presented by BookNet Canada on May 28, 2024, with support from the Department of Canadian Heritage.
Slack (or Teams) Automation for Bonterra Impact Management (fka Social Soluti...Jeffrey Haguewood
Sidekick Solutions uses Bonterra Impact Management (fka Social Solutions Apricot) and automation solutions to integrate data for business workflows.
We believe integration and automation are essential to user experience and the promise of efficient work through technology. Automation is the critical ingredient to realizing that full vision. We develop integration products and services for Bonterra Case Management software to support the deployment of automations for a variety of use cases.
This video focuses on the notifications, alerts, and approval requests using Slack for Bonterra Impact Management. The solutions covered in this webinar can also be deployed for Microsoft Teams.
Interested in deploying notification automations for Bonterra Impact Management? Contact us at sales@sidekicksolutionsllc.com to discuss next steps.
Search and Society: Reimagining Information Access for Radical FuturesBhaskar Mitra
The field of Information retrieval (IR) is currently undergoing a transformative shift, at least partly due to the emerging applications of generative AI to information access. In this talk, we will deliberate on the sociotechnical implications of generative AI for information access. We will argue that there is both a critical necessity and an exciting opportunity for the IR community to re-center our research agendas on societal needs while dismantling the artificial separation between the work on fairness, accountability, transparency, and ethics in IR and the rest of IR research. Instead of adopting a reactionary strategy of trying to mitigate potential social harms from emerging technologies, the community should aim to proactively set the research agenda for the kinds of systems we should build inspired by diverse explicitly stated sociotechnical imaginaries. The sociotechnical imaginaries that underpin the design and development of information access technologies needs to be explicitly articulated, and we need to develop theories of change in context of these diverse perspectives. Our guiding future imaginaries must be informed by other academic fields, such as democratic theory and critical theory, and should be co-developed with social science scholars, legal scholars, civil rights and social justice activists, and artists, among others.
Kubernetes & AI - Beauty and the Beast !?! @KCD Istanbul 2024Tobias Schneck
As AI technology is pushing into IT I was wondering myself, as an “infrastructure container kubernetes guy”, how get this fancy AI technology get managed from an infrastructure operational view? Is it possible to apply our lovely cloud native principals as well? What benefit’s both technologies could bring to each other?
Let me take this questions and provide you a short journey through existing deployment models and use cases for AI software. On practical examples, we discuss what cloud/on-premise strategy we may need for applying it to our own infrastructure to get it to work from an enterprise perspective. I want to give an overview about infrastructure requirements and technologies, what could be beneficial or limiting your AI use cases in an enterprise environment. An interactive Demo will give you some insides, what approaches I got already working for real.
LF Energy Webinar: Electrical Grid Modelling and Simulation Through PowSyBl -...DanBrown980551
Do you want to learn how to model and simulate an electrical network from scratch in under an hour?
Then welcome to this PowSyBl workshop, hosted by Rte, the French Transmission System Operator (TSO)!
During the webinar, you will discover the PowSyBl ecosystem as well as handle and study an electrical network through an interactive Python notebook.
PowSyBl is an open source project hosted by LF Energy, which offers a comprehensive set of features for electrical grid modelling and simulation. Among other advanced features, PowSyBl provides:
- A fully editable and extendable library for grid component modelling;
- Visualization tools to display your network;
- Grid simulation tools, such as power flows, security analyses (with or without remedial actions) and sensitivity analyses;
The framework is mostly written in Java, with a Python binding so that Python developers can access PowSyBl functionalities as well.
What you will learn during the webinar:
- For beginners: discover PowSyBl's functionalities through a quick general presentation and the notebook, without needing any expert coding skills;
- For advanced developers: master the skills to efficiently apply PowSyBl functionalities to your real-world scenarios.
Let's dive deeper into the world of ODC! Ricardo Alves (OutSystems) will join us to tell all about the new Data Fabric. After that, Sezen de Bruijn (OutSystems) will get into the details on how to best design a sturdy architecture within ODC.
JMeter webinar - integration with InfluxDB and GrafanaRTTS
Watch this recorded webinar about real-time monitoring of application performance. See how to integrate Apache JMeter, the open-source leader in performance testing, with InfluxDB, the open-source time-series database, and Grafana, the open-source analytics and visualization application.
In this webinar, we will review the benefits of leveraging InfluxDB and Grafana when executing load tests and demonstrate how these tools are used to visualize performance metrics.
Length: 30 minutes
Session Overview
-------------------------------------------
During this webinar, we will cover the following topics while demonstrating the integrations of JMeter, InfluxDB and Grafana:
- What out-of-the-box solutions are available for real-time monitoring JMeter tests?
- What are the benefits of integrating InfluxDB and Grafana into the load testing stack?
- Which features are provided by Grafana?
- Demonstration of InfluxDB and Grafana using a practice web application
To view the webinar recording, go to:
https://www.rttsweb.com/jmeter-integration-webinar
JMeter webinar - integration with InfluxDB and Grafana
Recycling of agricultural wastes in to bio fuel production: An ecofriendly product
1. International Journal of Engineering Inventions
e-ISSN: 2278-7461, p-ISSN: 2319-6491
Volume 3, Issue 3 (October 2013) PP: 60-65
Recycling of agricultural wastes in to bio fuel production: An
ecofriendly product
Devendra Pratap Singh1, Rakesh Kumar Trivedi2
1
(Department of Applied Chemistry,Dr. A.I.T.H, Kanpur 208024,. India)
(Professor & Head, Department of Oil and Paint Technology H. B.T.l., Kanpur 208002)
2
ABSTRACT: Carbohydrate biomass is considered as the future feedstock for bioethanol production because
of its low cost and its huge availability. In ancient years ethanol was manufactured by corn and starch, now in
these days ethanol is produced by lignocellulosic biomasses and algae in a large excess. Lignocellulose
contains carbohydrates units. The major carbohydrate materials found in great quantities to be considered,
especially in tropical countries, is wheat bran, sugarcane bagasse and rape straw, which can be easily
converted in to ethanol by following pretreatment either by acid or enzyme, hydrolysis and distillation process
under feasible conditions. The effects of different pH and temperature with enzymatic saccharification treatment
on conversion of these biomasses were studied. The produced glucose was fermented to bioethanol, using
Saccharomyces cerevisiae yeast in combination with pentose fermenting enzymes as Pitchia stipititis and the
amount of produced bioethanol was measured by gas chromatography. Enzyme treatment at 30ºC and pH 5 is
an effective treatment method for converting lignocelluloses to glucose. Up to 23.35% glucose v/v could be
achieved after enzyme treatment from bagasse than others. Fermentation of treated lignocelluloses shown that
glucose after 3 days fermentation the maximum bioethanol of 19.25% (v/v) by Saccharomyces cerevisiae and
26.75% (v/v) was attained in case of sugarcane bagasse by using Pitchia stipititis in combination with S.
cerevisiae. In this paper comparative study of enzymatic treatment and acid treatment is also analyzed. This
process is expected to be useful for the bioethanol production from wheat bran, sugarcane bagasse and rape
straw as a source of carbohydrate renewable biomass from abundant agricultural by product.
Keywords: Biomass, Carbohydrates, Fermentation, Pretreatment, Saccharification.
I.
INTRODUCTION
In the past few years there have been important advances in the field of alternative transportation fuels,
primarily bio ethanol and biodiesel. Only biodiesel and bio ethanol are considered in this report due to their
similar inherent properties compared to fossil-based fuels, especially auto ignitibility. There is a longer-term
potential for other bio fuels such as biobutanol and biogas but little research effort has been seen in either
regular or small engines. Bio ethanol is an alcohol, made by fermenting any biomass with a high content of
carbohydrates through a process similar to beer brewing. Today, bio ethanol is made from starches and sugars.
In the future, cellulose and hemicellulose fibrous material will be used. For the production of ethanol as bio
fuels, pre-treatment is the initial process to degrade the biomass into carbohydrates, so that it may be converted
in to monomers and latter on fermentation of sugars is the valuable process by which ethanol is obtained.
Chemical treatment is generally used to remove lignin content of agro residues. Chemical pre-treatment by
alkali or acid hydrolysis are common in paper and pulp industries to recover cellulose for paper production.
These treatments tend to be expensive hence are not used for bioconversion purposes. Pretreatment with acid or
alkali is common chemical method that has the effect of increasing the surface area of the agro-industrial residue
due to the swelling and disruption of lignin. Pretreatment is the pre step to release the components of
lignocellulosic biomass. Agro- residues consist of lignocelluloses that is compact, partly crystalline structure
consisting of linear and crystalline polysaccharides cellulose, branched non cellulosic and non-crystalline hetero
polysaccharides (hemicelluloses), and branched (non crystalline) lignin [1]. During the process some of the
compounds are formed which inhibits the direct fermentation, so it is necessary to remove them so that an
efficient products form. Detoxification of dilute-acid lignocelluloses hydrolysates by treatment with Ca(OH) 2
(over liming) efficiently improves the production of fuel ethanol, but is associated with drawbacks like sugar
degradation and CaSO4 precipitation. In factorial designed experiments, in which pH and temperature were
varied, dilute-acid spruce hydrolysates were treated with Ca(OH)2, NH4OH or NaOH. The concentrations of
sugars and inhibitory compounds were measured before and after the treatments. The ferment ability was
examined using the yeast Saccharomyces cerevisiae and compared with reference fermentations of synthetic
medium without inhibitors [2].
Biodiesel is made by combining alcohol (usually bio ethanol) with vegetable oil, animal fat, or
recycled cooking grease. These materials contain triglycerides and other components depending on type. Some
www.ijeijournal.com
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2. Recycling of agricultural wastes in to bio fuel production: an ecofriendly product
of the feedstocks are palm oil, coconut oil, canola oil, corn oil, cottonseed oil, flex oil, soy oil, peanut oil,
sunflower oil, rapeseed oil and algae. It can be used as an additive to reduce vehicle emissions or in its pure
form as a renewable alternative fuel for diesel engines. In the near future, agricultural residues such as corn
Stover (the stalks, leaves, and husks of the plant) and wheat straw will also be used. Biodiesel is one of the
future fuels for the engines that help to reduce the global warming and other harmful pollutants. Converting
vegetable oils into their esters of methyl and ethyl alcohols is known as Bio-diesel. Ester formation is a possible
way to overcome almost all the problems associated with vegetable oils. By the process of esterifications the
high viscosity of vegetable oils could be brought down to acceptable limits. Esters can be produced from oils
and fats by 3 methods. I) Base catalyzed trans-esterifications of oil with alcohol. ii) Direct acid catalyzed
esterifications of oil with methanol. iii) Conversion of oil to fatty acids and then to alkyl esters with acid
catalysts. The first method is preferred because it is economical. The conversion of vegetable oil (Triglyceride
Esters) to methyl esters through Tran’s esterifications process, this reduces the molecular weight to one-third,
reducing the viscosity by a factor of 8 and increasing the volatility. Vegetable oil is mixed with alcohol, NaOH
as catalyst. The mixture is heated and maintained at 650C for one hour, while heating, the solution is stirred
continuously with stirrer. Two distinct layers are formed, the lower layer is glycerin and the upper layer is ester.
The upper layer is separated with moisture and the ester is removed by using calcium chloride. It is observed
that 90% of ester is obtained from vegetable oil. Experimental research has been done on various blends of
gasoline and ethanol, and results have shown that 7.5% addition of ethanol was the best suitable for SI engine
with reduced CO emission [3].
Biodiesel reduces particulate, carbon monoxide, and sulphur dioxide
emissions compared to diesel fuel.
Lignocellulose is an important structural component of woody plants and consists of cellulose,
hemicelluloses, and lignin. The cellulose molecules, which are polymers with six carbon sugars linked in long
chains, and five carbon sugar chains called hemicelluloses, are reinforced by cross linked organic molecules
called lignin. This structure is very difficult for microbes to break down into simple sugars. In order to exploit
the structural sugars from plant fibers for bioethanol, the recalcitrance of biomass must be overcome in a
way that is cost competitive in current markets. The kind of cost- reducing measures that bio-refineries are working
towards are efficient de-polymerization of cellulose and hemicelluloses, efficient fermentation (including
pentose and hexose sugars) that can handle inhibitory compounds, optimizing process integration, and a cost
efficient use of lignin [18,19]. Table1 shows the composition of different biomass.
Lignocellulosic substrate Cellulose (%)
Hemicellulose (%) Lignin (%)
Sugarcane bagasse
45
35
15
Wheat straw
30
50
15
Rice straw
32.1
24
18
Rape straw
33.4
30
17
Rice bran
30.4
22
16
Wheat bran
31.3
23
17.5
Table 1. Effect of pH on glucose content of sugarcane baggase
In recent years, bio fuels producers have achieved significant improvements in crop production and
processing efficiencies and today the volume of bio fuels produced in a specific planted area is several times
Higher than it used to be. Improved production methods and technologies are expected to increase efficiencies
even further. In a simplified way, bio fuels can be sub-divided into two large categories: substitute for diesel
(biodiesel) and substitute for petroleum (ethanol). This division is based on the key properties of the two
products. On the one hand, biodiesel (which replaces diesel in cars) is produced from oil rich plants (e.g.
rapeseed, sunflower, algae, etc.) by mixing the vegetable oil (90%) with methanol (10%) in the process called
trans-esterifications. On the other hand, bio ethanol (which replaces petrol in cars) – also known as alcohol - is
produced through the fermentation of sugar from cereals (wheat, maize, etc.) or sugary feedstock’s (sugarcane,
sugar beet). Since biodiesel is derived from vegetable oils or animal fats made up of esters, these vegetable oils
are renewable biological sources. It has been reported that they emit substantially lower quantity of harmful
pollutants compare to conventional diesel; Researchers also found that comparable engine performance with
diesel was achieved at relatively lower emission [4, 5]. The merits of using biodiesel instead of conventional
diesel are has comparable energy density, cetane number, heat of vaporization, and stoichiometirc air /fuel ratio
[6]. Biodiesel is also non-toxic and rate of biodegradation is much faster than conventional diesel. Green house
gases effects were least in case of biodiesel [7, 8].
Use of Bio ethanol in motor engines is beneficial to the environment, because it produce CO2 from
combustion engines which is absorbed by plants these plants provide lignocelluloses, which turns in ethanol as
shown in Fig: 1, it is a recycle process which helps to control global warming and air pollutants. Another
environmental benefit of ethanol as a fuel is that the emission of carbon monoxide, nitrogen oxides and
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3. Recycling of agricultural wastes in to bio fuel production: an ecofriendly product
hydrocarbons in general is less compared to gasoline [9]. However, ethanol-containing fuels will contribute to
an increased emission of formaldehyde and acetaldehyde [10]. Nevertheless, the environmental damage caused
by reactive aldehydes is far less than that of poly-nuclear aromatic compounds, which are emitted when gasoline
is combusted [9].
Figure: 1 Production of ethanol from lignocelluloses
The choice of microorganism and strain is very important not only for high sugar utilization, ethanol
tolerance, and ethanol producing properties, but also for the robustness and the ability to withstand inhibitors.
The selection of microorganism may be influenced by its ability to ferment pentose sugars. The fraction of
pentose sugars is generally low in softwood hydrolysates, but if hardwood or agricultural residues are
considered, the ability to ferment Pentoses becomes more important. There are several naturally occurring
microorganisms that can utilize pentoses. E. coli is a natural pentose fermenter, but it gives low ethanol yields.
This problem has been addressed by genetic engineering to increase the ethanol production. S. cerevisiae and
Z. mobilis are excellent ethanol producers, but they are unable to metabolize Pentoses. Recombinant strains that
are able to utilize pentose sugars have been developed for both Z. mobilis and S. cerevisiae studied by [11, 12].
However, the genetically engineered pentose-fermenting S. cerevisiae strains have not yet been especially
successful; this is due to problems like low pentose fermentation rates, low ethanol yields, and redox imbalances
[13].
II.
MATERIAL AND METHODS
2.1 Material: Wheat bran, sugarcane bagasse, Rice bran and rape straw were used for the treatment process.
Before pretreatment these biomasses are reduced in to smaller particles after milling and crushing (particle size
<180 μm). Glucose was obtained from hydrolysis of wheat bran, sugarcane bagasse, Rice bran and rape straw.
After the hydrolysis hydrolyzed biomass were kept at 18°C until use [14].
2.2 Dilute Acid pre-treatment: For the comparative result equal amount of biomasses were pretreted in two
ways one is acid trement where 2-3% acid (H2SO4) was used for the pre-treatment method. In this content acid
soaked biomass slurry was autoclaved at 1210C for 30 minutes. To separate the solid and liquid fraction
centrifuge method was used. The dilution and pH was maintained at 5 by adding alkali of centrifuged biomass
before fermentation process.
2.3 Enzyme pre-treatment: 150 g of each biomass were suspended in 500 mL H2O in ratio of 3:10 (w/v)
sugarcane bagasse and added of 0.1 mL of α-amylase enzyme. The pH of sample was adjusted at pH 5, 5.5, and
6. The sample was incubated in water bath 100°C for 30 minutes, after that the mixture was applied for second
enzymatic treatment (0.2 ml of glucoamylase). Finally, hydrolzsate was pressed through cheese cloth. The
amount of reducing sugar in juice was measured.
2.4 Fermentation: The pre-treated samples from 2.2 and 2.3 were carried out for fermentation experiments. The
yeast S. cerevisiae was used for fermentation (1.5g, 3.0g, and 4.5g). After 3 fermentation days the ethanol
content was measured by gas chromatography. All the measurements were duplicated and the data reported are
average of two replications. Yeast S. cerevisiae was also used with Pitchia stipititis for both the fermentation of
pentose and hexose. Equal amount of both the yeast and P. Stipititis were taken for the efficient hydrolysis and
fermentation of both Pentoses and hexoses sugar present in the hydrolyzed.
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4. Recycling of agricultural wastes in to bio fuel production: an ecofriendly product
III.
RESULT AND DISCUSSION
3.1. Effect of enzyme pre-treatment methods on glucose content of sugarcane baggase: For the maximum
production of ethanol hydrolyzed biomass (both acid and enzyme pretreated) were fermented at different ph
scale, after fermentation the maximum result was obtained at pH 5 for the sugarcane bagasse. From the Table: 2,
it is clear that, increasing pH showed reverse effect on glucose concentration in sample. This is expected
because of conversion of carbohydrate to glucose [15]. The highest glucose up to 23.35 % glucose on the
sugarcane bagasse basis could be obtained on pH 5. Hence ph 5 at 300C was found optimum for fermentation;
hence it was taken for all the hydrolyzed biomass for fermentation.
pH
5
5.5
6
5
5.5
6
Temperature (°C)
30
30
30
40
40
40
Glucose (%)
23.35
22.80
22.00
21.05
19.43
18.95
Table 2. Effect of pH on glucose content of sugarcane baggase
3.2. Effect of acid pre-treatment methods on glucose content of sugarcane baggase: 150 g of each biomass
were suspended in 500 mL dilute acid of different concentration in the ratio of 3:10 (w/v) sugarcane bagasse.
The percentage of cellulose converted and the available form of substrate after enzymatic hydrolysis of various
concentrations of acid pre-treated corncob samples were calculated based on the amount of sugar released. The
results are presented in the Table 3. The results show that the percentage of cellulose conversion increased with
the increased concentrations of acid used for pre-treatment and it was also noted that the available form of
substrate reduced with increased concentrations of acid. Since maximum sugars were obtained at 3% acid pretreatment (optimum), hence it was used for further pre-treatment of other biomasses.
Sample
S1
S2
S3
S4
S5
S6
Sample-Acid used
treatment (%)
0.5
1.0
1.5
2.0
2.5
3.0
for
Pre-
Cellulose
Conversion (%)
11.8
12.8
13.6
14.2
15.2
16.0
Available
(%)
88.2
87.2
86.4
85.8
84.8
84
Substrate
Table 3. Effect of acid pretreatment on carbohydrate content of sugarcane baggase
3.3. Fermentation of enzyme treated biomass: Bio ethanol was produced by using S. cerevisiae and S.
cerevisiae in combination with Pitchia stipititis at pH 5 and 300C. 3.0g of each enzyme were taken which yields
maximum ethanol 19.25% by using S. cerevisiae while 26.75% was found by using S. cerevisiae and p.
stipititis, for sugarcane baggase. It can be seen in Figure 2 that pH 5.0 tended to give higher ethanol
concentration at 300C. Therefore, pH 5.0 was chosen for ethanol fermentation. This was supported by [16].
Biomass (150g)
Sugar (%)
Ethanol (%) by
Sugarcane baggase
Wheat Bran
Rape Straw
Rice bran
23.35
20.74
22.75
21.03
S.cerevisiae
19.25
17.47
18.09
17.95
S.cerevisiae & P. stipititis
26.75
21.17
25.48
24.28
Table 4. Production of Ethanol % (v/v) at 300C and pH 5 of enzyme treated biomass.
Production of bio ethanol in Table 2 shows that pH 5 at temperature 60°C had higher quantities of bio
ethanol produced and bio ethanol content of 19.25%, 17.47%, 18.09%, 17.95% (v/v) was recorded maximum
for bagasse, wheat bran rape straw and rice bran. This confirms that the higher sugar content in the juice, the
more bio ethanol can be produce which was also found similar according to the [17]. It was observed that the
sugar content was directly proportional to the quantities of the ethanol collected. Therefore, the higher the sugar
content, the more the ethanol can be produced [17]. By using Pitchia stipititis in combination with S. cerevisiae
maximum ethanol was found 26.75%, 21.17%, 25.48% and 24.28% for sugarcane bagasse, wheat bran rape
straw and rice bran. The high yield of ethanol production is due to the conversion of pentose sugar by P.
stipititis, because S. cerevisiae is only able to ferment hexose sugar as shown in fig: 2.
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5. Recycling of agricultural wastes in to bio fuel production: an ecofriendly product
40
35
Ethanol (%)
30
26.75
Glucose (%
)
25.48
25
24.28
21.17
Ethanol (% by S. cerevisie
)
Ethanol (% by P.Stitipititis and
)
S. cerevisie
20
15
10
Sugercane bagasse
Rape straw
Wheat bran
Typpes of biomass
Rice bran
Fig. 2 Production of Ethanol % (v/v) at 300C and pH 5from enzyme treated biomass
3.4. Fermentation of acid treated biomass: The feasibility of bio-alcohol production of cellulosic wastes
especially sugarcane baggase was made in this study. From the previous studies it was noted that increased acid
concentration used for the pretreatment purpose enhanced the sugar release during enzymatic hydrolysis.
Fermentation studies also proved that increased acid concentration yielded more amount of alcohol. Alcohol
content of about 25.38% (Table 5) was detected in the 72 hours of fermentation broth containing the hydrolysed
sample pretreated with 3% sulphuric acid. This study proved that sugars released by means of enzymatic
hydrolysis of pretreated bagasse are of fermentable category and alcohol can be produced efficiently using a
suitable fermenting strain Saccharomyces cerevisiae. Table-5 represents the total Ethanol Production from the
kinds of agricultural wastes. The comparative study of production of ethanol from acid treated and enzyme
treated biomasses are presented in Fig. 3. Maximum ethanol was found by acid treatment than enzymatic
treatment because in acid treatment both Pentoses and hexoses sugar was present.
SN.
Types of biomass samples
1
2
3
4
Sugarcane baggase
Wheat Bran
Rape Straw
Rice bran
Ethanol %(v/v) by
S.cerevisiae
24.25
21.47
23.95
23.05
S.cerevisiae & P. stipititis
35.38
31.25
34.37
33.98
Table 5. Production of Ethanol % (v/v) at 300C and pH 5 of acid treated biomass.
40
35.38
34.37
35
33.98
31.25
Glucose (%
)
Ethanol (%)
30
Ethanol (% by S. cerevisie
)
25
Ethanol (% by P.Stitipititis and
)
S. cerevisie
20
15
10
Sugercane bagasse
Rape straw
Wheat bran
Rice bran
Typpes of biomass
Fig. 3 Production of Ethanol % (v/v) at 300C and pH 5from Acid treated biomass
It is clear from the fig.2 and fig.3 that acid treated biomass yields maximum ethanol but there may be
chance of formation of inhibitors so that experiments were done very carefully. The maximum ethanol was
counted as 35.38% for sugarcane bagasse by fermentation with S.cerevisiae & P. stipititis of acid treated, while
in case of enzyme treated biomass it was 26.75%.
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