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![RECOMMENDATIONS FOR USING DEWORMERS
IN COMBINATION
Valbazen® Cydectin® Prohibit®*
Sheep 1.5 ml/50 lbs.
[7 days]
4.5 ml/50 lbs.
[7 days]
Depends on dilution
[3 days]
Goats 4 ml/50 lbs.
[9 days meat]
[7 days milk]
9 ml/50 lbs.
[17 days meat]
[8 days milk]
Depends on dilution
[4 days meat]
[3 days milk]](https://image.slidesharecdn.com/florida-parasites-171013125348/75/Internal-Parasites-Update-40-2048.jpg)
![COPPER REQUIREMENTS
• Sheep: old copper requirements were 7-11 ppm
(mg/kg), or 1-8.6 ppm, depending upon
physiological state of animal [NRC, 1985]
• New requirements use factorial method (equation)
to determine copper requirements [NRC, 2007];
equation considers absorption coefficients,
antagonists (Mo, S, Fe, Cd, Zn), and interactions.
• Same data is not available for goats; requirements
are set at 15, 20, and 25 ppm for lactating,
mature, and growing goats respectively;
maximum tolerable amount is unknown.
• Goats have higher copper requirements than
sheep and are less susceptible to copper toxicity.](https://image.slidesharecdn.com/florida-parasites-171013125348/75/Internal-Parasites-Update-46-2048.jpg)
![FECAL EGG COUNTING
www.wormx.info
[Consortium]
[W4: 2015 Conference]
[Fecal egg counts: uses and limitations]](https://image.slidesharecdn.com/florida-parasites-171013125348/75/Internal-Parasites-Update-53-2048.jpg)
Internal Parasites Update
This document provides information from a presentation on internal parasites for sheep given at the Meat Sheep Alliance of Florida Fall Symposium. The presentation covered topics including the American Consortium for Small Ruminant Parasite Control, an overview of common parasites, anthelmintics (dewormers), anthelmintic resistance, and alternative parasite control methods. It discussed common gastrointestinal parasites of sheep, described various anthelmintic drug classes and resistance issues, and emphasized the importance of integrated parasite management to prevent further development of anthelmintic resistance.
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Internal Parasites Update
- 1. AN INTERNAL PARASITEUPDATE MEAT SHEEP ALLIANCE OF FLORIDA FALL SYMPOSIUM OCAL A , FLORIDA - OCTOBER 14, 2017 SUSAN SCHOENIAN Sheep & Goat Specialist University of Maryland Extension sschoen@umd.edu - (301) 432-2767 x343 www.wormx.info - www.sheepandgoat.com https://www.slideshare.net/schoenian
- 2. PRESENTATION TOPICS • AmericanConsortium for Small Ruminant Parasite Control (ACSRPC) • Parasite overview • Anthelmintics (dewormers) 101 • Anthelmintic resistance • Combination treatments • Copper oxide wire particles • Nematode trapping fungus • Fecal egg counting
- 3. AMERICAN CONSORTIUM FORSMALL RUMINANT PARASITE CONTROL A group of scientists, veterinarians, and extension specialists devoted to (1) developing novel methods for sustainable control of gastro-intestinal nematodes in small ruminants and (2) educating the stakeholders in the small ruminant industry on the most up-to-date methods and recommendations for control of gastrointestinal nematodes.
- 4. WEB SITE WORMX.INFO ORACSRPC.ORG Go-to place for information about internal parasites. Not Facebook or Google!
- 5. ONLINE FAMACHA© TRAINING •Two members of consortium are piloting online FAMACHA© Training. • Dr. Katherine Petersson, University of Rhode Island • Dr. Anne Zajac, Virginia Tech • You view 2.5 hours of video: Integrated Parasite Control and Why and How to Do FAMACHA© scoring. • You Make a video of yourself demonstrating your FAMACHA© technique and send to URI. • Have follow-up with URI, if needed. • Upon completion receive certificate of competence and right to purchase FAMACHA© card(s). http://web.uri.edu/sheepngoat/famacha/
- 6. GASTRO-INTESTINAL PARASITES THAT AFFECTSMALL RUMINANTS
- 7. OVERVIEW • Gastro-intestinal parasitesare the primary health problem affecting sheep and goats worldwide. • GI parasites can be an obstacle to profitable and sustainable small ruminant production in many production systems. • Small ruminants are more susceptible to parasites than other farm livestock • Goats are more susceptible than sheep. • Problem is worsened by drug resistance.
- 8. MULTI-CELLULAR HELMINTHS 1. Nemadodes Roundworms Strongyles 2. Cestodes Flatworms Tapeworms 3.Trematodes Flukes 1. Coccidia 2. Giardia 3. Cryptospordia SINGLE CELL PROTOZOA MANY DIFFERENT PARASITES CAN AFFECT SHEEP AND GOATS.
- 9. PRIMARY 1. Haemonchus contortus Barberpole worm 2. Trichostrongylus spp. Black scour worm (bankrupt worm) 3. Teladorsagia (Ostertagia) Brown stomach worm 1. Cooperia small intestinal worm 2. Nematodirus threadneck worm 3. Oesophagostomu m nodule worm 4. Bunostomum hook worm SECONDARY NEMATODES – ROUNDWORMS - STRONGYLES 5. Trichuris ovis whipworm 6. Strongyloides threadworms 7. Lungworms 8. Parelaphostrongylus tenuis Meningeal worm
- 10. PRIMARY ROUNDWORMS • Haemonchuscontortus Barber pole worm • Trichostrongylus spp. Black scour worm • Teladorsagia (Ostertagia) Brown stomach worm Eggs look the same; need to hatch larvae in order to differentiate species. Trichostrongylus/Teladorsagia are often not differentiated even when doing larvae ID.
- 11. BARBER POLE WORM HAEMONCHUSCONTORTUS • Anemia: blood/protein loss Low packed cell volume (PCV) FAMACHA© score 3, 4, or 5 • Sub-mandibular edema “bottle jaw” swelling under jaw • Loss of weight and condition • Scours (diarrhea) • Weakness • Anorexia • Death • Acute haemonchosis: SUDDEN DEATH • Hypersensitivity of gut Damage and inflammation of gut Diarrhea (scours) • Loss of weight and condition Slow growth – poor performance • Lethargy • Death (sometimes) • Additive: usually part of mixed infections with H. contortus. “SCOUR WORMS” TRICHOSTRONGYLES TELADORSAGIA CLINICAL SIGNS (SYMPTOMS)
- 12. ROUNDWORMS CAN BE HARDTO CONTROL • Short, direct life cycles • No intermediate host • Ability to engage in hypobiosis • Barber pole worm is prolific egg layer • Lambs/kids slow to develop immunity. • Compromised immunity of periparturient female. • Widespread and growing drug resistance
- 13. COCCIDIA (EIMERIA SPP.) THEOTHER BIG PARASITE PROBLEM • Single-cell protozoan parasite. • More complex life cycle than roundworms. • Host-specific • Not all species are pathogenic (harmful). • Affects lambs/kids mostly before and after weaning. • Sheep develop strong and lifetime immunity; goats probably less so. • Causes diarrhea, but not always, and general ill- thrift • Associated with poor hygiene and management. https://attra.ncat.org/attra- pub/summaries/summary.php?pub=483
- 14. PREVENTION • Good hygiene,nutrition, and management. • Coccidiostats in mineral, feed, or water Need to feed or put in water before risk period 1. Lasalocid (Bovatec®) - sheep 2. Monensin (Rumensin®) - goats ( horses) 3. Decoquinate (Deccox®) - both 4. Amprolium (Corid®) - ELDU, OTC • Natural control • Sericea lespedeza pellets • Other (?) • Amprolium (Corid®)- ELDU, OTC • Sulfa drugs (Di-Methox®) - ELDU, Rx, VFD Damages lining of small intestines. Damage can be permanent (“runts”) TREATMENT COCCIDIOSIS
- 15. WHAT ABOUT TAPEWORMS? MONIEZIAEXPANSA • Tapeworms are the only parasite visible in the feces. Diagnosed by seeing segments in feces. • Tapeworms tend to be non-pathogenic; not harmful, but they’re blamed for a lot, usually no benefit to treatment (goats?). • Lambs develop immunity at very young age. • Tapeworms can cause mild unthriftiness and digestive disturbances, intestinal blockages (rare) and affect gut motility, predisposing lambs to enterotoxemia (occasionally). • Treat with albendazole (Valbazen®) or praziquantel (in some horse dewormers, ELDU) https://www.wormx.info/tapeworms
- 16. WHAT ABOUT MENINGEALWORM? PARALAPHOSTRONGYLUS TENIUS • Parasite of white tail deer. • Also called deer or brain worm. • Sheep, goats, alpacas, and llamas are all abnormal hosts. • Infection requires an intermediate host, terrestrial snail or slug. • Causes various neurological symptoms. • No diagnostic test in live animal. • No FDA-approved or proven treatment. • Cornell University has been evaluating treatment protocols: fenbendazole (SafeGuard®) + anti-inflammatory drugs (Dexamethasone or Banamine). https://nydairyadmin.cce.cornell. edu/uploads/doc_392.pdf
- 17.
- 18. WHAT IS ANANTHELMINTIC? • Compounds used to kill gastro-intestinal parasites without harming host. • More commonly called wormers, dewormers or drenches. • Most anthelmintics have a wide margin of safety. • Anthelmintics have different chemistries. • Chemistry determines which worms they are effective against, mode of action, and withdrawal period(s). • Anthelmintics are grouped by chemistries.
- 19. THERE ARE THREE(SORT OF 4) “CHEMICAL” CLASSES OF DEWORMERS FOR SMALL RUMINANTS (IN THE US). GROUP 1 Benzimidazoles (BZ) GROUP 2 Macrocylic lactones (ML) GROUP 3 Nicotinic agonists Avermectins Milbemycins Imidazo-thiazoles Tetrahydro-pyrimidines Fenbendazole SafeGuard® Panacur® Ivermectin Ivomec® Moxidectin Cydectin® Quest® Levamisole Prohibit® Leva-Med® Tramisol® Levasol® Morantel tartrate Rumatel® Albendazole Valbazen® Doramectin Dectomax® Pyrantel Strongid® Eprinomectin Eprinex® Oxfendazole Synanthic®
- 20. ANTHELMINTICS FDA-APPROVED FORSHEEP 1 Benzimidazoles Valbazen® sheep drench 2a Avermectins Ivomec® sheep drench 2b Milbimycins Cydectin® sheep drench 3 Levamisole Prohibit® Leva-Med® Adult worms Larvae (L4) Limited Hypobiotic larvae Limited Lungworms Tapeworms Liver flukes Adult stage Coccidia External parasites Some labeled for bot control Some Not labeled Persistent activity Safety 10x pregnancy restriction (first 30 days) 20x 5x 3x Labeled Dosage 3 ml/100 lbs. 3 ml/26 lbs. 1 ml/11 lbs. Depends on dilution 2 ml/50 lbs (concentrated drench) Meat withdrawal 7 days 11 days 7 days 3 days
- 21. ANTHELMINTICS FDA-APPROVED FORGOATS 1 Benzimidazoles 3b Morantel Fenbendazole SafeGuard® Albendazole Valbazen® Feed premix Rumatel Adult worms Not approved Larvae (L4) Not approved sporadic Hypobiotic larvae Not approved Lungworms Not approved Tapeworms Not approved Liver flukes Adult stage Coccidia External parasites Persistent activity Safety wide 10x (sheep) pregnancy restriction ~20x (sheep) Dosage 1.2 ml/50 lbs. 4 ml/100 lbs. Varies by product Meat withdrawal 6 days 7 days 30 days Milk withdrawal 0 days
- 22. EXTRA-LABEL ANTHELMINTICS FORGOATS 1 Benzimidazoles 2a Avermectins Ivomec® sheep drench 2b Milbimycins Moxidectin Cydectin® sheep drench 3a Levamisole Prohibit® Leva-Med® Fenbendazole SafeGuard® Albendazole Valbazen® Adult worms Larvae (L4) Limited Hypobiotic larvae Limited Lungworms Tapeworms Liver flukes Adult stage Coccidia External parasites Some label for bot control Some Not labeled Persistent activity Safety wide 10x pregnancy restriction 20x 5x 3x Dosage 1.1 ml/25 lbs. 2 ml/25 lbs. 6 ml/25 lbs. 4.5 ml/25 lbs. Depends on dilution Meat withdrawal 16 days (1 day for each additional day used) 9 days 14 days 17 days 4 days Milk withdrawal 4 days (1 day for each additional day used) 7 days 9 days 8 days 3 days
- 23.
- 24. IN WORM • Wormsdevelop resistance to drugs. Can’t be killed! Pass resistant genes onto next generation. • Some animals are more resistant to parasites. Ability to reduce parasite establishment. Pass resistance genes onto next generation. IN ANIMAL TWO KINDS OF RESISTANCE WHEN WE TALK ABOUT INTERNAL PARASITES
- 25. WHAT IS ANTHELMINTICRESISTANCE? • Genetic ability of a worm to survive a dose of anthelmintic which would normally be effective. • Only worms that survive treatment carry genes that confer resistance. • Result of selection through exposure of worm population to an anthelmintic. • When more than 5 percent of worms are “drug tolerant”; i.e. failure to reduce FEC by 95% or more (some say 90%). http://www.scops.org.uk/what-is-resistance.html
- 26. ANTHELMINTIC RESISTANCE • Inthe US, worms have developed resistance to all dewormers and dewormer classes, though it varies by geographic region and farm. • Worse in Southeast due to increased parasite risk and need for deworming. • Worse on farms that frequently deworm or use improper deworming practices. • Most farms have resistance to at least two dewormers; some farms have resistance to all dewormers/classes. 0 20 40 60 80 100 Benzimidazoles Ivermectin Levamisole Moxidectin Maryland Virginia Georgia
- 27. 0 2 4 6 8 10 Susceptible Suspected resistance Low resistance Moderate resistance High resistance Full resistance Numberof farms (n=29) resistant to Benzimidazoles based on DrenchRite® tests Maryland Virginia
- 28. 0 1 2 3 4 5 6 7 Susceptible Suspected resistance Low resistanceModerate to high High to full resistance Number of farms (n=29) resistant to Ivermectin based on DrenchRite® tests Maryland Virginia Georgia
- 29. 0 1 2 3 4 5 6 7 Susceptible Low resistanceLow to moderate Moderate to high Number of farms (n=29) resistant to Moxidectin based on DrenchRite® tests Maryland Virginia Georgia
- 30. 0 1 2 3 4 5 6 7 8 9 Susceptible Suspected resistance Low resistanceModerate resistance High to full resistance Number of farms (n=29) resistant to Levamisole based on DrenchRite® tests Maryland Virginia Georgia
- 31. ANTHELMINTIC RESISTANCE IS INEVITABLE,BUT CAN BE DELAYED. Practices that accelerate development of resistant worms 1. Frequent deworming 2. Whole flock treatments 3. Calendar based treatments 4. Treat and move strategy 5. Rotating dewormers 6. Underdosing 7. Depositing drug in mouth instead of deep into oral cavity. 8. Use of persistent activity dewormers 9. Use injectable dewormers 10. Use pour-on dewormers. 11. Use of feed dewormers* 12. Improper storage of dewormers
- 32. FECAL EGG COUNT REDUCTIONTEST • Compare before and after fecal egg counts from same animals. • Old protocol compared post-treatment FECs of treated animals vs. control (untreated) group. • ~15 animals per drug tested • Minimum FEC of 250 epg*, preferably higher. • Can use individual or pooled (composite) samples. • Cost varies. Can learn to do yourself or send to parasitology lab. • Labor-intensive laboratory test that determines resistance to all dewormers and classes from a single pooled fecal sample (from ~10 animals). • Minimum FEC of 500 epg. • Also identifies larvae: % Haemonchus Trichostrongyles eggs look the same. • Test done exclusively by Dr. Ray Kaplan’s lab at the University of Georgia. • $450 per sample DRENCHRITE® TEST TWO WAYS TO TEST FOR ANTHELMINTIC RESISTANCE
- 33. Animal Before After% FECR 1 1000 100 90% 2 500 25 95% 3 6000 150 98% 4 4350 250 94% 5 3000 1000 67% 6 1200 400 67% 7 1500 200 87% 8 750 50 93% 9 1100 100 91% 10 3100 200 94% 11 2900 200 93% 12 475 200 58% 13 900 100 89% 14 1100 50 95% 15 300 0 100% Avg 1878 145 87% Fecal egg count reduction test (FECRT)
- 34. COMBINATION TREATMENTS Different drugs tokills same parasites. Not different drugs for different parasites.
- 35. “ THERE NOWIS VERY STRONG EVIDENCE THAT USING COMBINATION TREATMENT IS THE BEST METHOD FOR USING DEWORMERS AND SHOULD BE INSTITUTED ON ALL FARMS IMMEDIATELY.” DR. RAY KAPLAN, UNIVERSITY OF GEORGIA (JANUARY 2017) http://www.wormx.info/combinations
- 36. RATIONALE FOR COMBINATION TREATMENTS •Most farms have resistance to at least two of the three major groups of dewormers; some have resistance to all. • At first introduction, drug efficacy is over 99%. • Once efficacy falls below 95%, drug resistance is present, though drug is still useful for treatment. • As effectiveness of dewormer decreases (<95%), as it is used more, it provides less and less benefit to animals. • Below 50%, it is no longer effective as sole There is already resistance to Zolvix® in other countries. Zolvix® is not sold in US.
- 37. WHY GIVE COMBINATION TREATMENTS? •Contrary to popular belief, rotating between dewormers will not prevent resistance from developing. In fact, it will allow worms to develop resistance to multiple drugs simultaneously. It is no longer recommended. • Research done in New Zealand has shown that the best approach is to use several different dewormers at one time as a combination treatment. • When combined with “best management practices” (that help to maintain refugia), combination treatments may improve drug efficacy and result in a reversion back toward susceptibility. Most dewormers sold in New Zealand and Australia are combination products (multiple drug actives in same product); combination products are not available in US.
- 38. HOW DO COMBINATIONSWORK? • Unlike rotating drugs, there is an additive effect with each drug used in a combination treatment. • By achieving a higher efficacy, there are fewer resistant worms that survive treatment. • The sooner you start using combination treatments the better, as you achieve the greatest difference in the percentage of resistant survivors when efficacy of dewormers is high. Drug 1 Drug 2 Drug 3 Combo12 Combo123 80% 80% 80% 96.00% 99.20% 90% 90% 90% 99.00% 99.90% 60% 95% 98.00% 98.00% 60% 60% 95% 84.00% 99.20% 99% 99% 99.99% 99.99% 60% 60% 60% 84.00% 93.60% 50% 50% 50% 75.00% 87.50% 40% 40% 40% 64.00% 78.40% 95% 80% 20% 99.00% 99.20%
- 39. RECOMMENDATIONS FOR USING COMBINATIONTREATMENTS • Purchase and administer each dewormer separately in a separate syringe. • Do not mix dewormers. They are not chemically compatible. Only veterinarians have the right to compound medications. • Administer each dewormer at full dose based on an accurate weight. • Can give one drug immediately after the other. • Observe withdrawal period of drug with longest withdrawal period
- 40. RECOMMENDATIONS FOR USINGDEWORMERS IN COMBINATION Valbazen® Cydectin® Prohibit®* Sheep 1.5 ml/50 lbs. [7 days] 4.5 ml/50 lbs. [7 days] Depends on dilution [3 days] Goats 4 ml/50 lbs. [9 days meat] [7 days milk] 9 ml/50 lbs. [17 days meat] [8 days milk] Depends on dilution [4 days meat] [3 days milk]
- 41. SELECTIVE TREATMENT ISIMPORTANT. REFUGIA MUST BE MAINTAINED! • Do not give combination treatments to all animals in a flock or group. • Selectively treat clinically-parasitized animals to maintain refugia. • Refugia are worms that have not been exposed to drug. • Use FAMACHA© system, Five Point Check©, and Happy Factor™ to determine which animals need dewormed. • If you deworm all animals in a flock or group, you will simultaneously accelerate resistance to all drugs.
- 42. FAMACHA© • Use coloreye chart to assess level (1-5) of anemia and need for deworming. • Decision making tool for blood- feeding parasites only: barber pole worm. • Examine 5 points on animal’s body to determine need for deworming: 1) eye (anemia), 2) jaw (bottle jaw), 3) back (BCS), 4) tail (dags), and 5) nose (nasal bots) or coat (for goats). • Expands decision making abililty to include parasites other than blood feeders, e.g. scour worms. FIVE POINT CHECK© DECISION MAKING TOOLS FOR MAKING DEWORMING DECISIONS • Use performance, e.g. ADG, as a criteria for making deworming decisions. • Could also use milk production. • Untested for barber pole worm. HAPPY FACTOR™
- 43. COPPER OXIDE WIRE PARTICLES(COWPS) https://www.wormx.info/copper-oxide-wire- particles
- 44. WHAT ARE COPPEROXIDE WIRE PARTICLES (COWPS)? • Tiny metal rods of copper oxide (Cu2O). • Poorly absorbed, slow release form of copper versus copper sulfate which is very absorbable; therefore, greater potential for toxicity (especially in sheep). • Has been shown to reduce barber pole worm infections in sheep and goats. • Available as copper supplement (different brands) for cattle (12.5 and 25 g) and goats (2 and 4 g). https://www.wormx.info/copper-oxide-wire-particles
- 45. COPPER BASICS • Sheepand goats require copper in their diets. • Copper metabolism is very complicated, with several antagonists (interactions), including molybdenum, sulfur, zinc, cadmium, and iron. • Copper absorption is more important than copper concentration in diet. • Copper absorption rates vary from 90% in young animals to 10% in mature animals to less than 1% in the presence of antagonists.
- 46. COPPER REQUIREMENTS • Sheep:old copper requirements were 7-11 ppm (mg/kg), or 1-8.6 ppm, depending upon physiological state of animal [NRC, 1985] • New requirements use factorial method (equation) to determine copper requirements [NRC, 2007]; equation considers absorption coefficients, antagonists (Mo, S, Fe, Cd, Zn), and interactions. • Same data is not available for goats; requirements are set at 15, 20, and 25 ppm for lactating, mature, and growing goats respectively; maximum tolerable amount is unknown. • Goats have higher copper requirements than sheep and are less susceptible to copper toxicity.
- 47. ASSESS THE COPPER STATUSOF YOUR FLOCK/HERD • Sheep are highly susceptible to copper toxicity; narrow margin between requirement and excess; are your goats copper deficient? • Blood copper levels can be misleading • Excess copper accumulates in liver. • Collect liver and kidney samples for testing. • Frozen or chilled samples can be sent to Michigan State University for testing. https://www.animalhealth.msu.edu/
- 48. TIPS FOR USINGCOWPS AS A DEWORMER • Re-package cattle boluses into smaller doses for sheep, 0.5 or 1 g. • Goat boluses can also be repackaged. • Use the smallest dose needed to achieve effect. • Dose based on age not weight: mature vs. young. • Selectively treat animals showing clinical signs of Haemonchosis (FAMACHA© 4 or 5) https://www.wormx.info/cowp-safety
- 49. ANOTHER COMBINATION TREATMENT VALBAZEN®+ COWPs Treatment (10-23 lambs per Tx group) Efficacy (%FECR) No treatment (control) Increase Valbazen® (3 ml/50 lbs.) 20% COWP (2 g, Ultracruz™) 58% COWP (2 g, Copasure®) 12% Valbazen® + COWP 99% Similar results could be expected if COWPs were combined with other dewormers (e.g. Prohibit®). USDA-ARS, Booneville, Arkansas http://www.wormx.info/cowpcombo
- 50. THE FUNGUS MAYSOON BE AMONG US.
- 51. NEMATODE TRAPPING FUNGUS DUDDINGTONIAFLAGRANS • Duddingtonia flagrans is a nematophagous fungus, meaning that it traps, paralyzes, and consumes parasites. • Non-chemical, biological control of the free-living stage of nematode parasites. • Substantially reduces number of infective worm larvae, including multi-resistant larvae. • Is fed to grazing animals. Spores resist digestion. No effect on host animal. Passes through into manure. • Reduces amount of reinfection from contaminated pasture. Interrupts of life cycle. www.duddingtonia.co m
- 52. USING FUNGUS TOCONTROL PARASITES • Not commercially available. Not yet. • Dr. Jim Miller from Louisiana State University (retired) has been told that product (fungus) should be available sometime early 2018. • Will need to feed every day to maintain effect. • Cost may be issue. • Greatest application is probably zoo animals.
- 53. FECAL EGG COUNTING www.wormx.info [Consortium] [W4:2015 Conference] [Fecal egg counts: uses and limitations]
- 54. QUALITATIVE • Positive ornegative. Yes or no. - or + • Mix feces with flotation solution. Place cover slip on meniscus. Put on slide. • Estimates number of eggs in a fresh sample of manure: eggs per gram of feces (EPG). • Mix known amount of feces (2-4 g) with known amount of flotation solution (26-28 ml) • Fill chambers of McMaster slide QUANTITATIVE FECAL EGG COUNTS (FECS) • You can learn to do your own fecals or send sample to a parasitology lab. • Microscope (100x) Mechanical stage helpful • McMaster egg counting slide • Homemade flotation solution
- 55. THREE PRIMARY USESOF FECAL EGG COUNTS 1. Determine treatment efficacy by comparing before and after fecal egg counts from a group of animals (~n=15) • Determine drug resistance on your farm • Determine efficacy of alternative treatment(s) 2. Monitor pasture contamination • How fast is pasture contamination building up? • Determine when to move animals 3. Identify resistant and susceptible animals • Differentiate between resistance and resilience • Need sufficient parasite challenge to get data separation (at least 500 epg group average, 1000 epg better) By themselves, fecal egg counts are not a good diagnostic tool for making individual deworming decisions.
- 56. S USAN SCHOENIAN S H E E P & G OAT S P E C I A L I S T U N I V E R S I T Y O F M A R Y L A N D E X T E N S I O N S S C H O E N @ U M D. E D U – ( 3 0 1 ) 4 3 2 - 2 7 6 7 X 3 4 3 S H E E PA N D G OAT. CO M – W O R M X . I N F O Thank you for your attention. Questions? Comments? https://www.slideshare.net/schoenian