Embl clustalw
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This document provides information about EMBL, CLUSTALW, and how multiple sequence alignment can be performed using CLUSTALW. EMBL is a leading life sciences research institution located in the UK. CLUSTALW is a multiple sequence alignment software produced in 1994 that uses seeded guide trees and HMM profile-profile alignment. The document then describes how to use NCBI and BLAST to retrieve gene sequences and perform multiple sequence alignment using CLUSTALW online to analyze gene sequences and their phylogenetic relationships.
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polymerase chain reaction work-flow by Dr kelvin Agimogim.pptx
Letter From Santa Template Microsoft Word - Letter Dai
Letter From Santa Template Microsoft Word - Letter Dai
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Hplc seminar
This document provides an overview of high performance liquid chromatography (HPLC). It discusses the basic components and principles of HPLC, including the stationary and mobile phases, various modes (normal and reverse phase), and instrumentation. The objective is to understand the purification and analytical applications of HPLC. The document outlines the experimental procedures for HPLC separation and analysis using chromatograms. Advantages include high performance and automation, while disadvantages include cost and difficulty with co-elution. HPLC has various applications in fields like drug discovery, food analysis, forensics, and clinical research.
Composting
This document discusses composting and provides details about the process. Composting involves the biological degradation of organic waste by microorganisms like bacteria and fungi. It produces a nutrient-rich compost that improves soil quality. The composting process involves different stages as temperatures increase. A variety of microorganisms and enzymes play key roles in breaking down materials. Composting has advantages like improving soil structure and providing plant nutrients, while disadvantages include being time-consuming and potentially contaminating heavy metals.
Artificial heart transplantation
The document summarizes information about total artificial heart transplantation including:
- The surgical procedure involves removing the damaged heart and attaching an artificial heart made of biocompatible plastic.
- Risks include bleeding, infections, and blood clots. The artificial heart aims to temporarily support patients until a donor heart is available.
- The surgery requires opening the chest, stopping the native heart, and connecting the artificial heart to blood vessels using a heart-lung machine for support during the operation.
Circular dichroism
This document provides an overview of circular dichroism (CD) spectroscopy. It begins with a brief history and definitions. The key principles are that CD measures the differential absorption of left and right circularly polarized light, providing structural information. Instrumentation and experimental procedures are described. Applications include determining protein secondary structure and monitoring structural changes. Software for CD analysis is also discussed. Examples are provided to illustrate how CD can be used to analyze protein secondary structure and monitor DNA structural changes.
Cyber security review paper
This document summarizes a review paper on cyber security. It discusses the need for cyber security to protect individuals, businesses, and governments from attacks on digital infrastructure and data. The objectives of cyber security are outlined as confidentiality, integrity, and availability of data and systems. Various cyber threats are described such as malware, phishing, and keystroke logging. Remedies for improving cyber security are also presented, including firewalls, antivirus software, password managers, security tokens, and security suites.
Metabolic pathways analysis.
This document discusses metabolic pathway analysis. It defines metabolic pathways as series of consecutive enzyme reactions that produce specific products. It notes that pathways are irreversible and compartmentalization allows different cycles to operate in eukaryotes. The document outlines several methods to analyze pathways, including isotope labeling, genome-scale metabolic modeling, and structural kinetic modeling. It provides the example of glycolysis and describes applications like finding high yield pathways and determining active reactions.
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Recently uploaded
Describing and Interpreting an Immersive Learning Case with the Immersion Cub...
Current descriptions of immersive learning cases are often difficult or impossible to compare. This is due to a myriad of different options on what details to include, which aspects are relevant, and on the descriptive approaches employed. Also, these aspects often combine very specific details with more general guidelines or indicate intents and rationales without clarifying their implementation. In this paper we provide a method to describe immersive learning cases that is structured to enable comparisons, yet flexible enough to allow researchers and practitioners to decide which aspects to include. This method leverages a taxonomy that classifies educational aspects at three levels (uses, practices, and strategies) and then utilizes two frameworks, the Immersive Learning Brain and the Immersion Cube, to enable a structured description and interpretation of immersive learning cases. The method is then demonstrated on a published immersive learning case on training for wind turbine maintenance using virtual reality. Applying the method results in a structured artifact, the Immersive Learning Case Sheet, that tags the case with its proximal uses, practices, and strategies, and refines the free text case description to ensure that matching details are included. This contribution is thus a case description method in support of future comparative research of immersive learning cases. We then discuss how the resulting description and interpretation can be leveraged to change immersion learning cases, by enriching them (considering low-effort changes or additions) or innovating (exploring more challenging avenues of transformation). The method holds significant promise to support better-grounded research in immersive learning.
Travis Hills' Endeavors in Minnesota: Fostering Environmental and Economic Pr...
Travis Hills of Minnesota developed a method to convert waste into high-value dry fertilizer, significantly enriching soil quality. By providing farmers with a valuable resource derived from waste, Travis Hills helps enhance farm profitability while promoting environmental stewardship. Travis Hills' sustainable practices lead to cost savings and increased revenue for farmers by improving resource efficiency and reducing waste.
20240520 Planning a Circuit Simulator in JavaScript.pptx
Evaporation step counter work. I have done a physical experiment.
(Work in progress.)
waterlessdyeingtechnolgyusing carbon dioxide chemicalspdf
The technology uses reclaimed CO₂ as the dyeing medium in a closed loop process. When pressurized, CO₂ becomes supercritical (SC-CO₂). In this state CO₂ has a very high solvent power, allowing the dye to dissolve easily.
The binding of cosmological structures by massless topological defects
Assuming spherical symmetry and weak field, it is shown that if one solves the Poisson equation or the Einstein field
equations sourced by a topological defect, i.e. a singularity of a very specific form, the result is a localized gravitational
field capable of driving flat rotation (i.e. Keplerian circular orbits at a constant speed for all radii) of test masses on a thin
spherical shell without any underlying mass. Moreover, a large-scale structure which exploits this solution by assembling
concentrically a number of such topological defects can establish a flat stellar or galactic rotation curve, and can also deflect
light in the same manner as an equipotential (isothermal) sphere. Thus, the need for dark matter or modified gravity theory is
mitigated, at least in part.
Thornton ESPP slides UK WW Network 4_6_24.pdf
ESPP presentation to EU Waste Water Network, 4th June 2024 “EU policies driving nutrient removal and recycling
and the revised UWWTD (Urban Waste Water Treatment Directive)”
原版制作(carleton毕业证书)卡尔顿大学毕业证硕士文凭原版一模一样
原版纸张【微信：741003700 】【(carleton毕业证书)卡尔顿大学毕业证】【微信：741003700 】学位证，留信认证（真实可查，永久存档）offer、雅思、外壳等材料/诚信可靠,可直接看成品样本，帮您解决无法毕业带来的各种难题！外壳，原版制作，诚信可靠，可直接看成品样本。行业标杆！精益求精，诚心合作，真诚制作！多年品质 ,按需精细制作，24小时接单,全套进口原装设备。十五年致力于帮助留学生解决难题，包您满意。
本公司拥有海外各大学样板无数，能完美还原海外各大学 Bachelor Diploma degree, Master Degree Diploma
1:1完美还原海外各大学毕业材料上的工艺：水印，阴影底纹，钢印LOGO烫金烫银，LOGO烫金烫银复合重叠。文字图案浮雕、激光镭射、紫外荧光、温感、复印防伪等防伪工艺。材料咨询办理、认证咨询办理请加学历顾问Q/微741003700
留信网认证的作用:
1:该专业认证可证明留学生真实身份
2:同时对留学生所学专业登记给予评定
3:国家专业人才认证中心颁发入库证书
4:这个认证书并且可以归档倒地方
5:凡事获得留信网入网的信息将会逐步更新到个人身份内，将在公安局网内查询个人身份证信息后，同步读取人才网入库信息
6:个人职称评审加20分
7:个人信誉贷款加10分
8:在国家人才网主办的国家网络招聘大会中纳入资料，供国家高端企业选择人才
SAR of Medicinal Chemistry 1st by dk.pdf
In this presentation include the prototype drug SAR on thus or with their examples .
Syllabus of Second Year B. Pharmacy
2019 PATTERN.
Immersive Learning That Works: Research Grounding and Paths Forward
We will metaverse into the essence of immersive learning, into its three dimensions and conceptual models. This approach encompasses elements from teaching methodologies to social involvement, through organizational concerns and technologies. Challenging the perception of learning as knowledge transfer, we introduce a 'Uses, Practices & Strategies' model operationalized by the 'Immersive Learning Brain' and ‘Immersion Cube’ frameworks. This approach offers a comprehensive guide through the intricacies of immersive educational experiences and spotlighting research frontiers, along the immersion dimensions of system, narrative, and agency. Our discourse extends to stakeholders beyond the academic sphere, addressing the interests of technologists, instructional designers, and policymakers. We span various contexts, from formal education to organizational transformation to the new horizon of an AI-pervasive society. This keynote aims to unite the iLRN community in a collaborative journey towards a future where immersive learning research and practice coalesce, paving the way for innovative educational research and practice landscapes.
在线办理(salfor毕业证书)索尔福德大学毕业证毕业完成信一模一样
学校原件一模一样【微信：741003700 】《(salfor毕业证书)索尔福德大学毕业证》【微信：741003700 】学位证，留信认证（真实可查，永久存档）原件一模一样纸张工艺/offer、雅思、外壳等材料/诚信可靠,可直接看成品样本，帮您解决无法毕业带来的各种难题！外壳，原版制作，诚信可靠，可直接看成品样本。行业标杆！精益求精，诚心合作，真诚制作！多年品质 ,按需精细制作，24小时接单,全套进口原装设备。十五年致力于帮助留学生解决难题，包您满意。
本公司拥有海外各大学样板无数，能完美还原。
1:1完美还原海外各大学毕业材料上的工艺：水印，阴影底纹，钢印LOGO烫金烫银，LOGO烫金烫银复合重叠。文字图案浮雕、激光镭射、紫外荧光、温感、复印防伪等防伪工艺。材料咨询办理、认证咨询办理请加学历顾问Q/微741003700
【主营项目】
一.毕业证【q微741003700】成绩单、使馆认证、教育部认证、雅思托福成绩单、学生卡等！
二.真实使馆公证(即留学回国人员证明,不成功不收费)
三.真实教育部学历学位认证（教育部存档！教育部留服网站永久可查）
四.办理各国各大学文凭(一对一专业服务,可全程监控跟踪进度)
如果您处于以下几种情况：
◇在校期间，因各种原因未能顺利毕业……拿不到官方毕业证【q/微741003700】
◇面对父母的压力，希望尽快拿到；
◇不清楚认证流程以及材料该如何准备；
◇回国时间很长，忘记办理；
◇回国马上就要找工作，办给用人单位看；
◇企事业单位必须要求办理的
◇需要报考公务员、购买免税车、落转户口
◇申请留学生创业基金
留信网认证的作用:
1:该专业认证可证明留学生真实身份
2:同时对留学生所学专业登记给予评定
3:国家专业人才认证中心颁发入库证书
4:这个认证书并且可以归档倒地方
5:凡事获得留信网入网的信息将会逐步更新到个人身份内，将在公安局网内查询个人身份证信息后，同步读取人才网入库信息
6:个人职称评审加20分
7:个人信誉贷款加10分
8:在国家人才网主办的国家网络招聘大会中纳入资料，供国家高端企业选择人才
Applied Science: Thermodynamics, Laws & Methodology.pdf
When I was asked to give a companion lecture in support of ‘The Philosophy of Science’ (https://shorturl.at/4pUXz) I decided not to walk through the detail of the many methodologies in order of use. Instead, I chose to employ a long standing, and ongoing, scientific development as an exemplar. And so, I chose the ever evolving story of Thermodynamics as a scientific investigation at its best.
Conducted over a period of >200 years, Thermodynamics R&D, and application, benefitted from the highest levels of professionalism, collaboration, and technical thoroughness. New layers of application, methodology, and practice were made possible by the progressive advance of technology. In turn, this has seen measurement and modelling accuracy continually improved at a micro and macro level.
Perhaps most importantly, Thermodynamics rapidly became a primary tool in the advance of applied science/engineering/technology, spanning micro-tech, to aerospace and cosmology. I can think of no better a story to illustrate the breadth of scientific methodologies and applications at their best.
Sharlene Leurig - Enabling Onsite Water Use with Net Zero Water
Sharlene Leurig - Enabling Onsite Water Use with Net Zero WaterTexas Alliance of Groundwater Districts
Presented at June 6-7 Texas Alliance of Groundwater Districts Business Meetingbordetella pertussis.................................ppt
Bordettela is a gram negative cocobacilli spread by air born drop let
Shallowest Oil Discovery of Turkiye.pptx
The Petroleum System of the Çukurova Field - the Shallowest Oil Discovery of Türkiye, Adana
Unlocking the mysteries of reproduction: Exploring fecundity and gonadosomati...
The pygmy halfbeak Dermogenys colletei, is known for its viviparous nature, this presents an intriguing case of relatively low fecundity, raising questions about potential compensatory reproductive strategies employed by this species. Our study delves into the examination of fecundity and the Gonadosomatic Index (GSI) in the Pygmy Halfbeak, D. colletei (Meisner, 2001), an intriguing viviparous fish indigenous to Sarawak, Borneo. We hypothesize that the Pygmy halfbeak, D. colletei, may exhibit unique reproductive adaptations to offset its low fecundity, thus enhancing its survival and fitness. To address this, we conducted a comprehensive study utilizing 28 mature female specimens of D. colletei, carefully measuring fecundity and GSI to shed light on the reproductive adaptations of this species. Our findings reveal that D. colletei indeed exhibits low fecundity, with a mean of 16.76 ± 2.01, and a mean GSI of 12.83 ± 1.27, providing crucial insights into the reproductive mechanisms at play in this species. These results underscore the existence of unique reproductive strategies in D. colletei, enabling its adaptation and persistence in Borneo's diverse aquatic ecosystems, and call for further ecological research to elucidate these mechanisms. This study lends to a better understanding of viviparous fish in Borneo and contributes to the broader field of aquatic ecology, enhancing our knowledge of species adaptations to unique ecological challenges.
Phenomics assisted breeding in crop improvement
As the population is increasing and will reach about 9 billion upto 2050. Also due to climate change, it is difficult to meet the food requirement of such a large population. Facing the challenges presented by resource shortages, climate
change, and increasing global population, crop yield and quality need to be improved in a sustainable way over the coming decades. Genetic improvement by breeding is the best way to increase crop productivity. With the rapid progression of functional
genomics, an increasing number of crop genomes have been sequenced and dozens of genes influencing key agronomic traits have been identified. However, current genome sequence information has not been adequately exploited for understanding
the complex characteristics of multiple gene, owing to a lack of crop phenotypic data. Efficient, automatic, and accurate technologies and platforms that can capture phenotypic data that can
be linked to genomics information for crop improvement at all growth stages have become as important as genotyping. Thus,
high-throughput phenotyping has become the major bottleneck restricting crop breeding. Plant phenomics has been defined as the high-throughput, accurate acquisition and analysis of multi-dimensional phenotypes
during crop growing stages at the organism level, including the cell, tissue, organ, individual plant, plot, and field levels. With the rapid development of novel sensors, imaging technology,
and analysis methods, numerous infrastructure platforms have been developed for phenotyping.
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Describing and Interpreting an Immersive Learning Case with the Immersion Cub...
Describing and Interpreting an Immersive Learning Case with the Immersion Cub...
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Travis Hills' Endeavors in Minnesota: Fostering Environmental and Economic Pr...
20240520 Planning a Circuit Simulator in JavaScript.pptx
20240520 Planning a Circuit Simulator in JavaScript.pptx
waterlessdyeingtechnolgyusing carbon dioxide chemicalspdf
waterlessdyeingtechnolgyusing carbon dioxide chemicalspdf
The binding of cosmological structures by massless topological defects
The binding of cosmological structures by massless topological defects
Immersive Learning That Works: Research Grounding and Paths Forward
Immersive Learning That Works: Research Grounding and Paths Forward
Applied Science: Thermodynamics, Laws & Methodology.pdf
Applied Science: Thermodynamics, Laws & Methodology.pdf
Sharlene Leurig - Enabling Onsite Water Use with Net Zero Water
Sharlene Leurig - Enabling Onsite Water Use with Net Zero Water
bordetella pertussis.................................ppt
bordetella pertussis.................................ppt
Unlocking the mysteries of reproduction: Exploring fecundity and gonadosomati...
Unlocking the mysteries of reproduction: Exploring fecundity and gonadosomati...
Embl clustalw
- 1. EMBL: CLUSTALW PRESENTED BY: MAHESH KUMAR DEPARTMENT OF BIOTECHNOLOGY ROLL NO.:191506
- 2. EMBL • INTRODUCTION: EMBL (EUROPEAN MOLECULAR BIOGICAL INFORMATION) • THIS IS THE ONE OF THE WORLD’S LEADING RESEARCH INSTITUTIONS AND EUROPE FLAGSHIP LABORATORY FOR THE LIFE SCIENCES. • LOCATION-WELCOME TRUST GENOME CAMPUS IN HINXTONS,UK ALONG WITH WELCOME TRUST SANGER INSTITUTE. • FOUNDED IN -1974 • HISTORY-EMBL-NUCLEOTIDE SEQUENCE DATE LIBRARY. • EMBL COUNCIL VOTED FOR ESTABLISHING EBI.
- 3. CLUSTALW • THIS IS THE THIRD GENERATION, RELEASED IN 1994. • THIS IS PRODUCED BY JULIE D ,THOMSON ,TOBY AND GIBSON OF EUROPEAN MOLECULAR BIOLOGY LABORATORY GERMANY AND DESMAND HIGGINS OF EUROPEAN BIOINFORMATICS INSTITUTE CAMBRIDGE, UK AND AGROTHMICS. • THIS IS AN BASIC MULTIPLE ALIGNMENT PROGRAM THAT USES SEEDED GUIDE TREES AND HMM PROFILE-PROFILE
- 4. AIMS AND SCOPES • EMBL-EBI MAINTAINS A COMPREHENSIVE RANGE OF AVAILABLE AND UP TO DATE DATABASES WHICH COLLECTIVELY COVER THE FULL RANGE OF MOLECULAR BIOLOGY, FROM NUCLEOTIDE SEQUENCE TO FULL SYSTEM. • IT HAS A MANDATE TO MAKE IT’S TASK AND INFRASTRUCTURE FREELY AVAILABLE TO THE GLOBAL SCIENTIFIC COMMUNITY. • EMBL-EBI IS A CENTRAL PARTNER IN GLOBAL EFFORTS TO EXCHANGE INFORMATION,SET STANDARDS, DEVELOP NEW METHODS AND CUARATE COMPLEX INFORMATION.
- 5. TIME COMPLEXITY • CLUSTALW HAS A TIME COMPLEXITY OF O(N2) BECOME OF IT’S USE OF THE NEIGHBOURS JOINING METHOD. • IT SAVES THE SIGNIFICANT AMOUNT OF TIME. • IT TAKES ONE HOUR.
- 6. HOW WE CAN WORK ON CLUSTALW ON LAPTOP OR MOBILE: ANALYZE OF DATA • FIRST WE WILL GO ON NCBI WEBSITE. • WE ASSUME THAT WE HAVE A PQQC GENE. • THEN HERE WE SELECT THE ALL DATABASE OPTIONS AND SELECT THE NUCLEOTIDE OPTIONS IN THIS . • THEN,WE WRITE THE PQQC GENE IN SEARCH BAR. • AFTER THAT ,WE WILL SEE THE LIST OF PQQC GENE AND SELECT ONE OF THEM. • AFTER SELECT THE GENE ,WE CLICK ON PARTICULAR GENE AND GET A SEQUENCE OF THAT PARTICULAR GENE.THEN ,WE GO ON FASTA FORMAT AND GET A SEQUENCE OF THAT PARTICULAR GENE NUCLEOTIDE ON FASTA FORMAT. • THEN WE COPY THIS FAST SEQUENCES AND GO ON BLAST WEBSITE AND HERE,WE SELECT NUCLEOTIDE BLAST AND IN IT ,WE COPY THE FASTA SEQUENCE.
- 7. CONTINUOUS.... • RUN THE BLAST NUCLEOTIDE. • WE GET MANY DOWNLOADED FILES IN BLAST FORMAT (FASTA ALIGNED). • SELECTION GENE SEQUENCES AND DOWNLOAD. • THEN COPY THE DOWNLOAD. • THEN WE WILL GO ON WWW.GENOME.JP(MULTIPLE SEQUENCE ALIGNMENT CLUSTALW). • HERE WE WILL SEE A BOX. • PASTE THE SEQUENCE FROM BLAST IN IT. • THEN EXECUTE MULTIPLE SEQUENCE ALIGNMENT. • RESULTS IS READY.AFTER THIS WE CAN MAKE PHYLOGENETIC ANALYSIS. • ALL THIS PROCESS IS GIVEN AS A FIGURE IN NEXT SLIDES.
- 10. CONTINUOU S..
- 11. CONTINUOUS..
- 12. CONTINUOUS.. .
- 13. CONTINUOUS....
- 14. CONTINUOUS..
- 15. CONTINUOUS...
- 16. CONTINUOUS...
- 17. CONTINUOUS ...
- 18. CONTINUOUS. ..
- 19. CONTINUOUS..
- 20. ACCURACY AND RESULTS • ALGORITHM CLUSTALW USES PROVIDES A CLOSE TO OPTIMAL RESULTS ALMOST EVERY TIME. • HOWEVER , IT DOES EXCEPTIONALY WELL WHEN THE DATA SET CONTAINS SEQUENCES WITH VARIED DEGREE OF DIVERGENCE. • THIS IS BECAUSE IN DATA SET LIKE, THIS IS THE GUIDE TREE BECOMES LESS SENSITIVE TO NOISE. • BUT THERE IS A LEAST LOSE OF ACCURACY THAT OTHER SOFTWARE DOESN’T HAVE DUE TO THIS. • WHEN THIS TESTED AGAINST MAFFT,T-COFFEE,CLUSTAL OMEGA AND OTHER MSA,IT HAD LOWEST ACCURACY. • IT HAD THE LEAST RAM MEMORY.
- 21. APPLICATION • 1.USED FOR ALIGNING MULTIPLE NUCLEOTIDE PROTEIN SEQUENCE IN AN EFFICIENT MANNER. • 2.WE CAN USE IN MANUAL PRIMER DESIGNING WITH CLUSTALW. • 3.THEY ARE MANY VERSION OF THE CLUSTAL OVER THE DEVELOPMENT OF THE ALGORITHM ARE USED IN PRESENT TIME TO DO MULTIPLE SEQUENCE ALIGNMENT.
- 22. REFERENCE • HTTP://YOUTU.BE/QDJPUJI1LYU. • NOTE ALL THE GIVEN IMAGES FROM NCBI WEBSITE. • HTTP://WWW.NCBI.NLM.NIH.GOV/
- 23. •THANKING YOU