- Glycoproteins are proteins that contain saccharide components attached through glycosidic bonds. The saccharide components can range from 1-15% of the total molecular mass and increase protein polarity, solubility, and surface charge.
- Saccharide components are attached through O-glycosidic or N-glycosidic bonds and can contain various monosaccharides. There is diversity in both the protein and saccharide structures among glycoproteins.
- Major glycoprotein types include blood plasma proteins, mucus proteins, proteoglycans, and collagen. Proteoglycans have large saccharide components that contribute to tissue structure and properties.
What is Glycoprotein ?:
Glycoproteins are proteins that contain oligosaccharide chains (glycans) covalently attached to polypeptide side-chains.
This process is known as glycosylation.
The carbohydrate is attached to the protein during the following modifications: Co-translational modification & Post-translational modification.
In proteins that have segments extending extracellularly, the extracellular segments are often glycosylated.
What is Glycoprotein ?:
Glycoproteins are proteins that contain oligosaccharide chains (glycans) covalently attached to polypeptide side-chains.
This process is known as glycosylation.
The carbohydrate is attached to the protein during the following modifications: Co-translational modification & Post-translational modification.
In proteins that have segments extending extracellularly, the extracellular segments are often glycosylated.
This file include these contents:
What is Triacylglycerol
Structure of triacylglycerol
Simple triacylglycerol
Mixed triacylglycerol
Biosynthesis of triacylglycerol
Utilization of triacylglycerol
Properties of triacylglycerol
Introduction
Definition
Classification of polysaccharides
1- Homopolysaccharides
2-Heteropolysaccharides
What is heteropolysaccarides?
Type of heteropolysaccharides
Function of heteropolysaccharides
Conclusion
References
Intermediary metabolism of carbohydrate,protein and fatSumair Arain
Most dietary carbohydrates are polymers of hexoses,primarily glucose, galactose and fructose.
Glucose is stored in its phosphorylated form glucose-6-phosphate; the formation of which in muscles is catalyzed by hexokinase, and in the liver by glucokinase.
Glucokinase is important because its activity is stimulated by insulin and its activity reduced in starvation, and glucokinase has no stronger affinity for glucose than hexokinase.
This file include these contents:
What is Triacylglycerol
Structure of triacylglycerol
Simple triacylglycerol
Mixed triacylglycerol
Biosynthesis of triacylglycerol
Utilization of triacylglycerol
Properties of triacylglycerol
Introduction
Definition
Classification of polysaccharides
1- Homopolysaccharides
2-Heteropolysaccharides
What is heteropolysaccarides?
Type of heteropolysaccharides
Function of heteropolysaccharides
Conclusion
References
Intermediary metabolism of carbohydrate,protein and fatSumair Arain
Most dietary carbohydrates are polymers of hexoses,primarily glucose, galactose and fructose.
Glucose is stored in its phosphorylated form glucose-6-phosphate; the formation of which in muscles is catalyzed by hexokinase, and in the liver by glucokinase.
Glucokinase is important because its activity is stimulated by insulin and its activity reduced in starvation, and glucokinase has no stronger affinity for glucose than hexokinase.
Carbohydrates are a class of biomolecules that are important sources of energy and structural components in living organisms. They are made up of carbon, hydrogen, and oxygen atoms, and they are classified based on their size and the number of sugar units they contain.
Cellular structures such as ribosomes, chromosomes, membranes, flagella, and cell walls are made up of ordered arrays of linear polymers or Macromolecules.
Macromolecules are constructed by covalently bonding monomers by condensation reactions where water is removed from functional groups on the monomers.
This presentation gives an overview of Lipid Rafts, how it was discovered, its importance and the future research in this area,Feel free to comment and ask any questions
Key Trends Shaping the Future of Infrastructure.pdfCheryl Hung
Keynote at DIGIT West Expo, Glasgow on 29 May 2024.
Cheryl Hung, ochery.com
Sr Director, Infrastructure Ecosystem, Arm.
The key trends across hardware, cloud and open-source; exploring how these areas are likely to mature and develop over the short and long-term, and then considering how organisations can position themselves to adapt and thrive.
Accelerate your Kubernetes clusters with Varnish CachingThijs Feryn
A presentation about the usage and availability of Varnish on Kubernetes. This talk explores the capabilities of Varnish caching and shows how to use the Varnish Helm chart to deploy it to Kubernetes.
This presentation was delivered at K8SUG Singapore. See https://feryn.eu/presentations/accelerate-your-kubernetes-clusters-with-varnish-caching-k8sug-singapore-28-2024 for more details.
State of ICS and IoT Cyber Threat Landscape Report 2024 previewPrayukth K V
The IoT and OT threat landscape report has been prepared by the Threat Research Team at Sectrio using data from Sectrio, cyber threat intelligence farming facilities spread across over 85 cities around the world. In addition, Sectrio also runs AI-based advanced threat and payload engagement facilities that serve as sinks to attract and engage sophisticated threat actors, and newer malware including new variants and latent threats that are at an earlier stage of development.
The latest edition of the OT/ICS and IoT security Threat Landscape Report 2024 also covers:
State of global ICS asset and network exposure
Sectoral targets and attacks as well as the cost of ransom
Global APT activity, AI usage, actor and tactic profiles, and implications
Rise in volumes of AI-powered cyberattacks
Major cyber events in 2024
Malware and malicious payload trends
Cyberattack types and targets
Vulnerability exploit attempts on CVEs
Attacks on counties – USA
Expansion of bot farms – how, where, and why
In-depth analysis of the cyber threat landscape across North America, South America, Europe, APAC, and the Middle East
Why are attacks on smart factories rising?
Cyber risk predictions
Axis of attacks – Europe
Systemic attacks in the Middle East
Download the full report from here:
https://sectrio.com/resources/ot-threat-landscape-reports/sectrio-releases-ot-ics-and-iot-security-threat-landscape-report-2024/
Epistemic Interaction - tuning interfaces to provide information for AI supportAlan Dix
Paper presented at SYNERGY workshop at AVI 2024, Genoa, Italy. 3rd June 2024
https://alandix.com/academic/papers/synergy2024-epistemic/
As machine learning integrates deeper into human-computer interactions, the concept of epistemic interaction emerges, aiming to refine these interactions to enhance system adaptability. This approach encourages minor, intentional adjustments in user behaviour to enrich the data available for system learning. This paper introduces epistemic interaction within the context of human-system communication, illustrating how deliberate interaction design can improve system understanding and adaptation. Through concrete examples, we demonstrate the potential of epistemic interaction to significantly advance human-computer interaction by leveraging intuitive human communication strategies to inform system design and functionality, offering a novel pathway for enriching user-system engagements.
Neuro-symbolic is not enough, we need neuro-*semantic*Frank van Harmelen
Neuro-symbolic (NeSy) AI is on the rise. However, simply machine learning on just any symbolic structure is not sufficient to really harvest the gains of NeSy. These will only be gained when the symbolic structures have an actual semantics. I give an operational definition of semantics as “predictable inference”.
All of this illustrated with link prediction over knowledge graphs, but the argument is general.
Encryption in Microsoft 365 - ExpertsLive Netherlands 2024Albert Hoitingh
In this session I delve into the encryption technology used in Microsoft 365 and Microsoft Purview. Including the concepts of Customer Key and Double Key Encryption.
GDG Cloud Southlake #33: Boule & Rebala: Effective AppSec in SDLC using Deplo...James Anderson
Effective Application Security in Software Delivery lifecycle using Deployment Firewall and DBOM
The modern software delivery process (or the CI/CD process) includes many tools, distributed teams, open-source code, and cloud platforms. Constant focus on speed to release software to market, along with the traditional slow and manual security checks has caused gaps in continuous security as an important piece in the software supply chain. Today organizations feel more susceptible to external and internal cyber threats due to the vast attack surface in their applications supply chain and the lack of end-to-end governance and risk management.
The software team must secure its software delivery process to avoid vulnerability and security breaches. This needs to be achieved with existing tool chains and without extensive rework of the delivery processes. This talk will present strategies and techniques for providing visibility into the true risk of the existing vulnerabilities, preventing the introduction of security issues in the software, resolving vulnerabilities in production environments quickly, and capturing the deployment bill of materials (DBOM).
Speakers:
Bob Boule
Robert Boule is a technology enthusiast with PASSION for technology and making things work along with a knack for helping others understand how things work. He comes with around 20 years of solution engineering experience in application security, software continuous delivery, and SaaS platforms. He is known for his dynamic presentations in CI/CD and application security integrated in software delivery lifecycle.
Gopinath Rebala
Gopinath Rebala is the CTO of OpsMx, where he has overall responsibility for the machine learning and data processing architectures for Secure Software Delivery. Gopi also has a strong connection with our customers, leading design and architecture for strategic implementations. Gopi is a frequent speaker and well-known leader in continuous delivery and integrating security into software delivery.
Slack (or Teams) Automation for Bonterra Impact Management (fka Social Soluti...Jeffrey Haguewood
Sidekick Solutions uses Bonterra Impact Management (fka Social Solutions Apricot) and automation solutions to integrate data for business workflows.
We believe integration and automation are essential to user experience and the promise of efficient work through technology. Automation is the critical ingredient to realizing that full vision. We develop integration products and services for Bonterra Case Management software to support the deployment of automations for a variety of use cases.
This video focuses on the notifications, alerts, and approval requests using Slack for Bonterra Impact Management. The solutions covered in this webinar can also be deployed for Microsoft Teams.
Interested in deploying notification automations for Bonterra Impact Management? Contact us at sales@sidekicksolutionsllc.com to discuss next steps.
Elevating Tactical DDD Patterns Through Object CalisthenicsDorra BARTAGUIZ
After immersing yourself in the blue book and its red counterpart, attending DDD-focused conferences, and applying tactical patterns, you're left with a crucial question: How do I ensure my design is effective? Tactical patterns within Domain-Driven Design (DDD) serve as guiding principles for creating clear and manageable domain models. However, achieving success with these patterns requires additional guidance. Interestingly, we've observed that a set of constraints initially designed for training purposes remarkably aligns with effective pattern implementation, offering a more ‘mechanical’ approach. Let's explore together how Object Calisthenics can elevate the design of your tactical DDD patterns, offering concrete help for those venturing into DDD for the first time!
Securing your Kubernetes cluster_ a step-by-step guide to success !KatiaHIMEUR1
Today, after several years of existence, an extremely active community and an ultra-dynamic ecosystem, Kubernetes has established itself as the de facto standard in container orchestration. Thanks to a wide range of managed services, it has never been so easy to set up a ready-to-use Kubernetes cluster.
However, this ease of use means that the subject of security in Kubernetes is often left for later, or even neglected. This exposes companies to significant risks.
In this talk, I'll show you step-by-step how to secure your Kubernetes cluster for greater peace of mind and reliability.
3. Saccharide components of glycoproteins are attached to the polypeptide chains through covalent glycosidic bonds , either O - glycosidic bonds of oligoglycosyls to alcoholic groups in the side chains of residues of serine , threonine or hydroxylysine , or N - glycosidic bonds of oligoglycosyls to the amide groups in the side chains of asparagine . Ser Asn
4. In certain glycoprotein types, more than two or three glycosyls can be attached through glycosidic bonds to one glycosyl so that branching occurs in those saccharide components. Because the saccharide component of glycoproteins may comprise many different glycosyl types , there is a very high degree of diversity in the structures of both protein and saccharide component of glycoproteins.
5.
6. The major glycoprotein types – Blood plasma glycoprotein type most of the blood plasma proteins (not plasma albumin!) and many integral membrane glycoproteins that form glycocalyx – Mucus glycoprotein type (mucins) secreted by epithelial cells with protective and lubricative functions (among others, also glycoproteins with blood AB0 group determining structures) – Proteoglycans – produced by fibroblasts – Collagen type – products of fibroblasts The major collagen types are glycosylated only very poorly (about 1 % of molecular weight)
7. Blood plasma glycoprotein type Oligosaccharides are attached to the amide group of asparaginyl residues through N -glycosidic bonds: Asn
8. The boxed area encloses the pentasaccharide core common to all N -linked glycoproteins. Bi-antennary type (complex type) High-mannose type is a common precursor in biosynthesis of other types
9. Integral membrane penetrating ( glyco ) proteins Type I Type II less common "reversed" type, e.g. transferrin receptor Type III PI-link Type IV e.g. superfamily of receptors interacting with G-proteins
10.
11.
12. The molecules can comprise up to 75 % saccharides forming so very viscous solutions (a typical feature of secretion from secretory cells of mucosa. Mucus glycoprotein type Antifreeze glycoproteins in antarctic fish prevents from freezing (inhibits ice nucleation) – galactosyl–N-acetyldeoxyaminogalactosyl serine / threonine Saccharide component is attached through O– glycosidic bond to the alcoholic groups of seryl or threonyl residues:
13. In the membranes or red blood cells, those antigenic determinants occur as glycolipids (attached to membrane ceramide). In some humans ( "secretors"), AB0 antigens appear as the saccharide component of glycoproteins secreted by epithelial cells.
14. Proteoglycans If not thinking of dense collagen connective tissue and bone, proteoglycans represent the most voluminous component of amorphous ground substance in connective tissue, which fill in the space among fibres and cells. In proteoglycans, numerous (very approximately 100) chains of different glycosaminoglycans (that include 10 –100 monosaccharide units) bind through glycosidic bonds the core protein forming so aggregates called monomeric proteoglycans or agrecans . The most typical link is the link of the innermost sequence of glycosaminoglycans – galactosyl–galactosyl– xylyl serine
15. A large number of simple monomeric proteoglycans (agrecans) bind their globular domains of core proteins non-covalently to a long chain of hyaluronic acid. Huge aggregates are formed in this way namely in hyaline cartilages. They contribute to the resistance of a cartilage to mechanical pressure and to its elasticity . Proteoglycans are highly hydrated , and numerous carboxylate and sulfate groups bind due to negative electric charges large amounts of hydrated cations. hyaluronate Monomeric proteoglycan (agrecan) agrecans In spite of its large size, core protein of proteoglycans represents only about 5 – 15 % mass of the proteoglycan. The agrecan structure resembles a bottle-brush. The bristles are N -linked oligosaccharides, O -linked oligosaccharides or keratan sulfates, O -linked Xyl-Gal-Gal-chondroitin sulfates.
16. Collagen type glycoproteins In collagen types I and III, small number of galactosyls or glucosyl-galactosyls are attached to 5- hydroxylysyl residues through O -glycosidic bonds : α-D-galactosyl hydroxylysine ( 2- O- -D-glucosyl )
17. Some structural divergences in collagen types I , II , III , and IV Collagen I is the most common type ( skin , bones , tendons, dentin), resisting to tensile strength. Slightly glycosylated ( < 1 % saccharides), no cysteinyl residues. Collagen II is the major type present in the hyaline cartilage of joints. High degree of glycosylation , no cysteinyl residues. Collagen III (skin, aorta, uterus) is an elastic type in the form of thin reticuline fibrils . Very low glycosylation, cysteinyl residues are present, small number of disulfide bridges. Collagen IV is the typical type of basement membranes (among others renal glomeruli, capsule of the eye lens) forming the non-fibrillar network that stabilizes a thin membrane. Its flexible triple helices include some non-helical segments and at their C-ends there are globular domains . Saccharidic component about 15 %, cysteinyl residues and disulfide bridges are present.
19. Haemoproteins are proteins with different functions containing covalently bound haem . (In American English, haem is spelled "heme".) Important haemoproteins: Haemoglobin transporting dioxygen (and its derivatives), myoglobin binding dioxygen within skeletal muscles, cytochromes of different types that transport electrons in the terminal respiratory chain (or similar electron transferring systems, e.g. cytochromes P 450), some enzymes catalyzing oxidations-reductions, for example catalase and peroxidase . Haem is an Fe-containing prosthetic group. The heterocyclic ring system of haem is a porphyrin derivative . It consists of four pyrrole rings linked by four methene bridges, with a centrally bound Fe(II) atom.
21. Porphin is a planar , fully conjugated system. N N N N H H Porphin Cyclic tetrapyrroles N N N N H H Pyrrole rings A B C D N N N N H H Methene bridges α N N N N H H Numbering of positions of substituents 2 3 (1) (20) 7 8 12 13 17 18
22. N HN N NH CH 3 COOH COOH CH 3 CH 3 CH 3 CH 2 CH 2 protoporphyrin IX Derivatives of porphin (by substitution) are porphyrins – intensively coloured, there is a fully conjugated system of double bonds, or porphyrinogens , in which some of the bridges are methylene bridges (much less absorption of visible light). Kinds of substituents: Two sorts – 4 remainders of acetic acid, 4 remainders of propionic acid ( uroporhyrinogens/uroporphyrins ), or – 4 methyl groups and 4 remainders of propionic acid ( coproporphyrinogens/coproporphyrins ); Three sorts – 4 methyl groups, 2 vinyl groups and 2 remainders of propionic acid ( protoporphyrins ).
23. The side chains that fill in the interhelical space are not drawn. The tertiary structure of haemoglobin subunit
24. Cytochromes are haem-containing proteins , which are one-electron carriers due to reversible oxidation of the iron atom: Mammalian cytochromes are of three types, called a , b , and c . All these types of cytochromes occur in the mitochondrial respiratory (electron transport) chain. Cytochromes type b (including cytochromes class P 450 ) occur also in membranes of endoplasmic reticulum and elsewhere. N N N N F e 2+ N N N N F e 3+ + e – – e –
25. Some differences in cytochrome structures Cytochrome c M r 12 000, the central Fe ion is attached by coordination to N-atom of His 18 and to S-atom of Met 80 ; two vinyl groups bind covalently S-atoms of Cys 14 and Cys 17 . The haem is dived deeply in the protein terciary structure so that it is unable to bind dioxygen, carbon monoxide or CN – ion. Cyt c is water-soluble, peripheral protein that moves on the outer side of the inner mitochondrial membrane. Cytochrome aa 3 M r ~ 170 000, the central Fe ion is attached by coordination to two histidyl residues; one of substituents is a hydrophobic isoprenoid chain, another one is oxidized to formyl group. The haem a is the accepts an electrons from the copper centre A (two atoms Cu A ). Its function is inhibited by carbon monoxide, CN – , HS – , and N 3 – anions. Haem a of cytochrome aa 3 Haem of cytochrome c
26. bilanes (with substituents at β-positions) CH 3 H H H O O N N N N H CH 3 CH 3 CH 3 CH 2 CH 2 HOOC COOH bilirubin IX α H H H O O N N N N H Linear tetrapyrroles The product of oxidative splitting of haem is green biliverdin, Fe 3+ ion and carbon monoxide.