The document discusses aseptic technique, which is a method aimed at preventing contamination from microorganisms, particularly in clinical settings such as surgeries and clinics. It covers the history, benefits, complications, and general precautions associated with aseptic techniques, highlighting the importance of preventing infection in vulnerable patients. References from relevant sources are provided to support the information presented.

1. TOPIC :- SEMINER ON ASEPTIC TECHNIQUE
PRESENTED BY :-
Tuhin Samanta

2. • INTRODUCTION
• HISTORY
• ASEPTIC TECHNIQUE BENEFITS
• ASEPTIC TECHNIQUE
COMPLICATION
• GENERAL PRECAUTION
• REEFERENCE
CONTENTS

3. INTRODUCTION
Aseptic technique is a method designed to prevent
contamination from microorganisms. It involves applying
the strictest rules and utilizing what is known about
infection prevention to minimize the risks that you’ll
experience an infection. Common settings where the aseptic
technique is used include surgery rooms, clinics, and
outpatient care centers.

4. What Is Aseptic Technique Used for ?
• Aseptic technique is used in various clinical settings to
prevent the spread of pathogens. The primary goal of the
aseptic technique is to prevent harmful organisms from
spreading and causing infection.
• Aseptic technique is commonly used in the following
situations:
• handling surgery equipment
• during vaginal labor
• accessing dialysis catheters
• performing dialysis

5. • inserting a chest tube
• inserting a urinary catheter
• inserting central intravenous (IV) or arterial lines
• inserting other draining devices
• performing various surgical techniques

6. History
The modern concept of asepsis evolved in the 19th century.
Ignaz Semmelweis showed that washing the hands prior to
delivery reduced puerperal fever. After the suggestion by
Louis Pasteur, Joseph Lister, 1st Baron Lister introduced the
use of carbolic acid as an antiseptic and reduced surgical
infections rates. Lawson Tait went from antisepsis to
asepsis, introducing principles and the iconic statutes that
have remained valid to this day. Ernst von Bergmann
introduced the autoclave, a device used for the practice of
the sterilization of surgical instruments.

7. Aseptic Technique Benefits
• Whenever our skin is opened, we’re vulnerable to infection. This is
why prompt treatment for burns and wounds is so critical. If the
exposure is intentional, such as in surgery, we’re also at risk for
infection. The way healthcare providers use aseptic techniques can
determine whether or not we’ll develop an infection from our
procedure.
• Patients needing surgery or other procedures that require aseptic
technique are already vulnerable to infections. They need their
immune system to be at its strongest to heal the body. A person has a
better chance of a recovery if they don’t have to fight off an acquired
infection.

8. Aseptic Technique Complications
There are several common kinds of healthcare-associated
infections (HAIs) that healthcare workers try to minimize
by using aseptic techniques. These include:
I) CAUTIs (pronounced caught-EASE)
II) central line-associated bloodstream infections
III) Clostridium difficile infections
IV) surgical site infections

9. General Precautions
• Avoid creating aerosols when pipetting or transferring liquids
• Handle cells gently
• Do not shake or pipet suspensions vigorously
• If you feel that you may have touched a sterile item
• Discard it and use a new one
• Clean up any spills immediately after decontaminating with
disinfectant

10. • Make sure caps are on tight before centrifuging cell
suspensions
• If possible use aerosol containment or wrap
parafilmaound the caps
• Avoid spinning cells too hard or for too long
• Pellet resuspension is best done gently, using small
volumes of medium, followed by dilution of the
suspension

11. REFERENCE
• University of Missouri, St. Louis Department of
Biology. Techniques and Troubleshooting. Science in
the Real World: Microbes in Action Program, 2010
• Briggs, M., Wilson, S. & Fuller, A. (1996) The
Principles of aseptic technique in wound care. Prof
Nurse, 11 (12), 805-8
• Davey, J.G. (1997) Discovering nursing students’
understanding about aseptic technique. Int J Nurs
Pract, 3(2), 105-10