Antibodies or immunoglobulin's are protein particles delivered by a specific gathering of cells called B-lymphocytes in creatures. These are a piece of the guard framework to ensure the body against the attacking outside substances to be specific antigens.
Monoclonal immunizer (Mab) is a solitary sort of neutralizer that is coordinated against a particular antigenic determinant (epitope). Eternal monoclonal counter acting agent are found in patients experiencing an infection called different myeloma. In 1975 George Kohler and Cesar Milstein were effectively hybridize counter acting agent delivering B-lymphocytes with myeloma cells in vitro and make a hybridoma. The creation of monoclonal immune response by half and half cells is alluded to as hybridoma innovation.
An antibody (Ab), also known as an immunoglobulin (Ig), is a large, Y-shaped protein produced mainly by plasma cells that is used by the immune system to neutralize pathogens such as pathogenic bacteria and viruses.
Monoclonal antibodies are important reagents used in biomedical research, in diagnosis of diseases, and in treatment of such diseases as infections and cancer.
These antibodies are produced by cell lines or clones obtained from animals that have been immunized with the substance that is the subject of study.
An antibody (Ab), also known as an immunoglobulin (Ig), is a large, Y-shaped protein produced mainly by plasma cells that is used by the immune system to neutralize pathogens such as pathogenic bacteria and viruses.
Monoclonal antibodies are important reagents used in biomedical research, in diagnosis of diseases, and in treatment of such diseases as infections and cancer.
These antibodies are produced by cell lines or clones obtained from animals that have been immunized with the substance that is the subject of study.
Affinity Chromatography involves the covalent attachment of an immobilized biochemical called as affinity ligand to a solid support. When a sample is passed through the column, only solute that selectively binds to the complementary ligand is retained; other sample components elute without retention. The separation exploit the “lock and key” binding that is prevalent in biological systems. The retention solutes can be eluted from the column by changing the mobile phase composition.
Various approaches to Targeted Drug Delivery Systems (TDDS) in its formuation and evaluation in a pharmaceutical industry and research is outlined in this presentation.
pharma industry is growing very fast after the IT sector. Now a days pharma industries are using robots in their companies to avoid man-made mistakes and to increase the work power and productivity.
Antisense oligonucleotides
Antisense therapy is a form of treatment that uses antisense oligonucleotides to target messenger RNA.
(AS ONs) are synthetic DNA oligomers that hybridize to a target RNA in a sequence-specific manner. They have successfully been employed to inhibit gene expression, modulate splicing of a precursor messenger RNA, or inactivate microRNAs.
This will provide you the introduction about the tumor, its Anatomy & Physiology,How they are monitored?, Classification and grades of tumor, Tumor Targeting Techniques, strategies and Principles. Also provide you some examples of Marketed products.
Affinity Chromatography involves the covalent attachment of an immobilized biochemical called as affinity ligand to a solid support. When a sample is passed through the column, only solute that selectively binds to the complementary ligand is retained; other sample components elute without retention. The separation exploit the “lock and key” binding that is prevalent in biological systems. The retention solutes can be eluted from the column by changing the mobile phase composition.
Various approaches to Targeted Drug Delivery Systems (TDDS) in its formuation and evaluation in a pharmaceutical industry and research is outlined in this presentation.
pharma industry is growing very fast after the IT sector. Now a days pharma industries are using robots in their companies to avoid man-made mistakes and to increase the work power and productivity.
Antisense oligonucleotides
Antisense therapy is a form of treatment that uses antisense oligonucleotides to target messenger RNA.
(AS ONs) are synthetic DNA oligomers that hybridize to a target RNA in a sequence-specific manner. They have successfully been employed to inhibit gene expression, modulate splicing of a precursor messenger RNA, or inactivate microRNAs.
This will provide you the introduction about the tumor, its Anatomy & Physiology,How they are monitored?, Classification and grades of tumor, Tumor Targeting Techniques, strategies and Principles. Also provide you some examples of Marketed products.
Polyclonal vs. monoclonal antibodies: Research, Medicine, and Diagnostics | T...The Lifesciences Magazine
Comprehending the polyclonal vs. monoclonal antibodies reveals the complexities of the human immune response and the uses of these potent immunological instruments.
Monoclonal Antibodies and it's applications.pptxAfroj Shaikh
SlideShare Description: Monoclonal Antibodies and Their Applications
In the rapidly advancing field of biotechnology, monoclonal antibodies have emerged as powerful tools with diverse applications. This SlideShare presentation provides a comprehensive overview of monoclonal antibodies and their wide-ranging uses in various fields, including medicine, research, and diagnostics.
The presentation begins by explaining the fundamental concept of monoclonal antibodies, highlighting their unique structure and production process. It delves into the significance of hybridoma technology, which allows for the generation of large quantities of identical antibodies derived from a single parental cell line.
Moving on, the SlideShare explores the applications of monoclonal antibodies in the field of medicine. It elucidates how these antibodies are employed in targeted therapies, such as cancer immunotherapy. The presentation highlights the remarkable specificity of monoclonal antibodies in recognizing and binding to specific targets, thereby enabling precise and tailored treatment approaches. It also discusses the role of monoclonal antibodies in autoimmune diseases, infectious diseases, and organ transplantation.
Furthermore, the presentation sheds light on the use of monoclonal antibodies in research and diagnostics. It explains how these antibodies are utilized as indispensable tools in laboratory research, facilitating the identification and characterization of various biomarkers and molecules. It also showcases their utility in techniques such as enzyme-linked immunosorbent assays (ELISA), flow cytometry, and immunohistochemistry.
The SlideShare emphasizes the impact of monoclonal antibodies on the development of novel therapeutic modalities, including antibody-drug conjugates and bispecific antibodies. It touches upon the challenges and future prospects in the field, highlighting ongoing research efforts and advancements in antibody engineering.
With visually appealing slides, concise and informative content, this SlideShare presentation on monoclonal antibodies provides a valuable resource for scientists, healthcare professionals, students, and anyone interested in understanding the significance and applications of these remarkable biotechnological innovations.
Antibodies, also known as immunoglobulins, are secreted by B cells (plasma cells) to neutralize antigens such as bacteria and viruses. The classical representation of an antibody is a Y-shaped molecule composed of four polypeptides-two heavy chains and two light chains. Each tip of the "Y" contains a paratope (a structure analogous to a lock) that is specific for one particular epitope (similarly analogous to a key) on an antigen, allowing these two structures to bind together with precision. The ability of binding to an antigen has led to their ubiquitous use in a variety of life science and medical science. These antibodies can be classified into two primary types (monoclonal and polyclonal) by the means in which they are created from lymphocytes. Each of them has important role in the immune system, diagnostic exams, and treatments.
CAR T-cell Therapy_A New Era in Cancer ImmunotherapyTuhin Samanta
Illusory Antigen Receptor (CAR) T-cell treatment includes hereditary alteration of patient's autologous T-cells to express a CAR explicit for a tumor antigen, following by ex vivo cell extension and re-imbuement back to the patient. Vehicles are combination proteins of a chose single-chain section variable from a particular monoclonal immune response and at least one T-cell receptor intracellular flagging spaces. This T-cell hereditary change may happen either by means of viral-based quality exchange strategies or nonviral techniques, for example, DNA-based transposons, CRISPR/Cas9 innovation or direct exchange of in vitro deciphered mRNA by electroporation.
Detection and Enumeration of Coliforms in Ganga WaterTuhin Samanta
MPN is most generally applied for quality testing of water i.e to guarantee whether the water is protected or not as far as microorganisms present in it. A gathering of microscopic organisms normally alluded as fecal coliforms go about as a pointer for fecal pollution of water. The nearness of not many fecal coliform microbes would show that a water presumably contains no disease‑causing living beings, while the nearness of huge quantities of fecal coliform microscopic organisms would demonstrate an extremely high likelihood that the water could contain disease‑producing life forms making the water hazardous for utilization.
The transesterification procedure is a reversible response and did by blending the reactants – unsaturated fats, liquor and impetus. A solid base or a solid corrosive can be utilized as an impetus. At the mechanical scale, for the most part sodium or potassium methanolate is utilized. The finished results of the transesterification procedure are crude biodiesel and crude glycerol. In a further procedure these crude items experience a cleaning step. If there should be an occurrence of utilizing methanol as liquor FAME (unsaturated fat methyl ester) biodiesel is delivered. The cleansed glycerol can be utilized in the nourishment and corrective ventures, just as in the oleochemical business. The glycerol can likewise be utilized as a substrate for anaerobic absorption.
Biodiesel is an elective fuel like regular or 'fossil' diesel. Biodiesel can be delivered from straight vegetable oil, creature oil/fats, fat and waste cooking oil. The procedure used to change over these oils to Biodiesel is called transesterification. This procedure is depicted in more detail beneath. The biggest conceivable wellspring of appropriate oil originates from oil yields, for example, rapeseed, palm or soybean. In the UK rapeseed speaks to the best potential for biodiesel creation. Most biodiesel created at present is delivered from squander vegetable oil sourced from eateries, chip shops, modern nourishment makers, for example, Birdseye and so forth. Despite the fact that oil directly from the horticultural business speaks to the best potential source it isn't being delivered economically essentially in light of the fact that the crude oil is excessively costly. After the expense of changing over it to biodiesel has been included it is basically too costly to even think about competing with fossil diesel. Squander vegetable oil can regularly be sourced for nothing or sourced effectively treated at a little cost.
Bioinformatics & It's Scope in BiotechnologyTuhin Samanta
As an interdisciplinary field of science, bioinformatics consolidates science, software engineering, data building, arithmetic and measurements to dissect and decipher organic information. Bioinformatics has been utilized for in silico investigations of organic inquiries utilizing numerical and measurable methods.
DNA fingerprinting is a research facility procedure used to set up a connection between natural proof and a suspect in a criminal examination. A DNA test taken from a wrongdoing scene is contrasted and a DNA test from a suspect. On the off chance that the two DNA profiles are a match, at that point the proof originated from that suspect. On the other hand, on the off chance that the two DNA profiles don't coordinate, at that point the proof can't have originated from the suspect. DNA fingerprinting is likewise used to build up paternity.
Literature Survey Antibiotic ResistanceTuhin Samanta
Anti-toxin obstruction happens when microscopic organisms change in light of the utilization of these medications. Microscopic organisms, not people or creatures, become anti-toxin safe. These microorganisms may contaminate people and creatures, and the diseases they cause are more diligently to treat than those brought about by non-safe microscopic organisms.
Rheology is the investigation of the progression of issue, fundamentally in a fluid state, yet in addition as "delicate solids" or solids under conditions in which they react with plastic stream as opposed to distorting flexibly because of an applied power. Rheology is the study of misshapening and stream inside a material.
LITERATURE SURVEY & MARKET SURVEY ON AMLODIPINE AND ITS RECENT TRENDSTuhin Samanta
Amlodipine is utilized with or without different drugs to treat hypertension. Bringing down hypertension forestalls strokes, respiratory failures, and kidney issues. Amlodipine has a place with a class of medications known as calcium channel blockers. It works by loosening up veins so blood can stream all the more effectively.
Literature Survey & Market Survey on Amlodipine and its Recent TrendsTuhin Samanta
Amlodipine is utilized with or without different drugs to treat hypertension. Bringing down hypertension forestalls strokes, respiratory failures, and kidney issues. Amlodipine has a place with a class of medications known as calcium channel blockers. It works by loosening up veins so blood can stream all the more effectively.
Soy items originate from soybeans. Soybeans are vegetables that have been a piece of Asian weight control plans for a considerable length of time. Soybeans are utilized to make tofu, soymilk, soy flour, miso and numerous different nourishment's. In contrast to other plant nourishments, soybeans have a high protein content, identical to creature food sources. Like meat, soy is a finished protein.
Aseptic strategy implies utilizing practices and methodology to keep pollution from pathogens. It includes applying the strictest standards to limit the danger of disease. Human services laborers utilize aseptic system in medical procedure rooms, facilities, outpatient care focuses, and other social insurance settings.
Hyperglycemia is the specialized term for high blood (glucose). High glucose happens when the body has too little insulin or when the body can't utilize insulin appropriately.
Hyperglycemia is a condition where the measure of flowing glucose (or sugar) in the circulation system is higher than ordinary. At the point when nourishment enters the stomach related framework, it is separated into glucose and enters the circulation system. This triggers a procedure that causes the pancreas to discharge insulin. Insulin is a hormone discharged by the pancreas that helps maneuver glucose into the cells of the body, where it is changed over into fuel and vitality.
Hyperglycemia is the specialized term for high blood (glucose). High glucose happens when the body has too little insulin or when the body can't utilize insulin appropriately.
June 3, 2024 Anti-Semitism Letter Sent to MIT President Kornbluth and MIT Cor...Levi Shapiro
Letter from the Congress of the United States regarding Anti-Semitism sent June 3rd to MIT President Sally Kornbluth, MIT Corp Chair, Mark Gorenberg
Dear Dr. Kornbluth and Mr. Gorenberg,
The US House of Representatives is deeply concerned by ongoing and pervasive acts of antisemitic
harassment and intimidation at the Massachusetts Institute of Technology (MIT). Failing to act decisively to ensure a safe learning environment for all students would be a grave dereliction of your responsibilities as President of MIT and Chair of the MIT Corporation.
This Congress will not stand idly by and allow an environment hostile to Jewish students to persist. The House believes that your institution is in violation of Title VI of the Civil Rights Act, and the inability or
unwillingness to rectify this violation through action requires accountability.
Postsecondary education is a unique opportunity for students to learn and have their ideas and beliefs challenged. However, universities receiving hundreds of millions of federal funds annually have denied
students that opportunity and have been hijacked to become venues for the promotion of terrorism, antisemitic harassment and intimidation, unlawful encampments, and in some cases, assaults and riots.
The House of Representatives will not countenance the use of federal funds to indoctrinate students into hateful, antisemitic, anti-American supporters of terrorism. Investigations into campus antisemitism by the Committee on Education and the Workforce and the Committee on Ways and Means have been expanded into a Congress-wide probe across all relevant jurisdictions to address this national crisis. The undersigned Committees will conduct oversight into the use of federal funds at MIT and its learning environment under authorities granted to each Committee.
• The Committee on Education and the Workforce has been investigating your institution since December 7, 2023. The Committee has broad jurisdiction over postsecondary education, including its compliance with Title VI of the Civil Rights Act, campus safety concerns over disruptions to the learning environment, and the awarding of federal student aid under the Higher Education Act.
• The Committee on Oversight and Accountability is investigating the sources of funding and other support flowing to groups espousing pro-Hamas propaganda and engaged in antisemitic harassment and intimidation of students. The Committee on Oversight and Accountability is the principal oversight committee of the US House of Representatives and has broad authority to investigate “any matter” at “any time” under House Rule X.
• The Committee on Ways and Means has been investigating several universities since November 15, 2023, when the Committee held a hearing entitled From Ivory Towers to Dark Corners: Investigating the Nexus Between Antisemitism, Tax-Exempt Universities, and Terror Financing. The Committee followed the hearing with letters to those institutions on January 10, 202
Embracing GenAI - A Strategic ImperativePeter Windle
Artificial Intelligence (AI) technologies such as Generative AI, Image Generators and Large Language Models have had a dramatic impact on teaching, learning and assessment over the past 18 months. The most immediate threat AI posed was to Academic Integrity with Higher Education Institutes (HEIs) focusing their efforts on combating the use of GenAI in assessment. Guidelines were developed for staff and students, policies put in place too. Innovative educators have forged paths in the use of Generative AI for teaching, learning and assessments leading to pockets of transformation springing up across HEIs, often with little or no top-down guidance, support or direction.
This Gasta posits a strategic approach to integrating AI into HEIs to prepare staff, students and the curriculum for an evolving world and workplace. We will highlight the advantages of working with these technologies beyond the realm of teaching, learning and assessment by considering prompt engineering skills, industry impact, curriculum changes, and the need for staff upskilling. In contrast, not engaging strategically with Generative AI poses risks, including falling behind peers, missed opportunities and failing to ensure our graduates remain employable. The rapid evolution of AI technologies necessitates a proactive and strategic approach if we are to remain relevant.
Operation “Blue Star” is the only event in the history of Independent India where the state went into war with its own people. Even after about 40 years it is not clear if it was culmination of states anger over people of the region, a political game of power or start of dictatorial chapter in the democratic setup.
The people of Punjab felt alienated from main stream due to denial of their just demands during a long democratic struggle since independence. As it happen all over the word, it led to militant struggle with great loss of lives of military, police and civilian personnel. Killing of Indira Gandhi and massacre of innocent Sikhs in Delhi and other India cities was also associated with this movement.
Palestine last event orientationfvgnh .pptxRaedMohamed3
An EFL lesson about the current events in Palestine. It is intended to be for intermediate students who wish to increase their listening skills through a short lesson in power point.
Biological screening of herbal drugs: Introduction and Need for
Phyto-Pharmacological Screening, New Strategies for evaluating
Natural Products, In vitro evaluation techniques for Antioxidants, Antimicrobial and Anticancer drugs. In vivo evaluation techniques
for Anti-inflammatory, Antiulcer, Anticancer, Wound healing, Antidiabetic, Hepatoprotective, Cardio protective, Diuretics and
Antifertility, Toxicity studies as per OECD guidelines
Instructions for Submissions thorugh G- Classroom.pptxJheel Barad
This presentation provides a briefing on how to upload submissions and documents in Google Classroom. It was prepared as part of an orientation for new Sainik School in-service teacher trainees. As a training officer, my goal is to ensure that you are comfortable and proficient with this essential tool for managing assignments and fostering student engagement.
Instructions for Submissions thorugh G- Classroom.pptx
Literature Review on Development of Monoclonal Antibodies and Hybridoma Technology in Recent Pharmaceutical Application
1. Literature Review on Development of Monoclonal
Antibodies and Hybridoma Technology in Recent
Pharmaceutical Application
Tuhin Samanta
2. INTRODUCTION
• Antibodies or immunoglobulin's are protein molecules
produced by a specialized group of cells called B-
lymphocytes in animals. These are a part of the
defense system to protect the body against the
invading foreign substances namely antigens.
• Each antigen has a specific antigen determinants
(epitopes)located on it. The antibodies have
complementary determining regions which are mainly
responsible for the antibody specificity. In response to
an antigen B-lymphocytes gear up and produce many
different antibodies which can react with same antigen
are known as polyclonal antibodies, which are
heterogenic in nature.
3. MONOCLONAL ANTIBODY
Monoclonal antibody (Mab) is a single type of antibody that is directed against a
specific antigenic determinant (epitope). Immortal monoclonal antibody are
found in patients suffering from a disease called multiple myeloma. In 1975
George Kohler and Cesar Milstein were successfully hybridize antibody-
producing B-lymphocytes with myeloma cells in vitro and create a hybridoma.
The production of monoclonal antibody by hybrid cells is referred to as
hybridoma technology.1
4. 0BJECTIVE OF THE STUDY
The monoclonal antibody and hybridoma technology play a very vital role
in the field of immunology.
Now a day’s many Mab products are used for therapeutic purpose for
detection of different disease and also for treatment of antibody deficiency
syndrome. So, this literature review has been undertaken to highlight the
formation and clinical use of this antibody in various pharmacological
purpose.
Sigma gene expression.
Immortal.
6. APPLICATION OF MONOCLONAL ANTIBODY
Serological
Identification of ABO blood groups
Diagnosis
Detection of pregnancy by assaying of hormones with monoclonal,
Detection of pathogens
Separation of one substance from a mixture of very similar molecules.
7. Immunopurification
Purification of individual interferon's using monoclonal
Inactivation of T lymphocytes responsible for rejection of organ transplants.
Therapy
Removal of tumor cells from bone marrow.
Neutralize the reaction or response by one defined antigen, but still preserve the
reaction of all other antigens.
Most widely used monoclonal is OKTI, particularly for the treatment of acute renal
allograft rejections.
Treatment of patients with malignant leukemic cells, B cell lymphomas, and a
variety of allograft rejections after transplantation.
8. LITERATURE REVIEW
1. Monoclonal Antibodies Present New Opportunities
in Disease Diagnosis and Treatment
The production of MAbs by hybridoma technology was discovered in
1975 by Georges Kohler of West Germany and Cesar Milstein of
Argentina. Who developed a technique to fuse splenocyte cells (separated
from the spleen of an immunized mouse) with timorous myeloma cells.,
our vision for antibodies as tools for research for prevention, detection
and treatment of diseases, vaccine production, antigenic characterization
of pathogens and in the study of genetic regulation of immune responses
and disease susceptibility has been revolutionized. Recently, MAbs have
been widely applied in the field of clinical medicine.2
9. 2. Monoclonal Antibodies: A tool in clinical
research
Monoclonal antibodies (MAbs) are an old immunological tool with
applications in the fields of immunology, biotechnology, biochemistry, and
applied biology. Modern-day research on MAbs from laboratories
worldwide is revealing additional applications in diverse branches of
sciences. Recently, MAbs have been widely applied in the field of clinical
medicine. Currently, MAbs account for one-third of all the new therapeutic
treatments for breast cancer, leukemia, arthritis, transplant rejection,
asthma, and psoriasis, with many more late-stage clinical trials being
conducted. The future prospect lies in the applicability of monoclonal
antibodies as a molecule for understanding and monitoring the biology of
disease and its role in research, clinical, diagnostic, analytical, and
pharmaceutical applications.3
10. 3. Application of Radionuclides and Antibody-Drug
Conjugates to Target Cancer
Radionuclide therapy and antibody-drug conjugates are used to locate and
kill cancer cells by the utilization of monoclonal antibodies. These bio-
vectors are able to transport a cytotoxic drug payload and/or radiation in the
form of alpha or beta particles to bind onto antigen specific cancer cells
initiating apoptosis.4
11. 4. Monoclonal antibodies: An emerging
immunotherapy technology
Antibodies of a single idiotype produced by immortalized B cells
are called monoclonal antibodies which are mono-specific in nature.
Its specificity serves as a detector for purify almost any given
substance. The Y shaped structure of antibodies consist of 2 heavy
chains and 2 light chains with one variable region which gives
antibody its specificity for binding antigen and 3 constant regions
determines the mechanism used to destroy antigen.5
12. 5. Production of Monoclonal Antibodies specific for
Progesterone
Progesterone levels in milk and serum are indicators of pregnancy in cattle.
The progesterone level reaches a peak on the 21st and 22nd days of
pregnancy. Monoclonal antibodies specific to progesterone could be used
for the immunodetection of milk and serum progesterone levels. 6
13. DRUG CONTAINING MONOCLONAL
ANTIBODY
SL NO NAME OF THE
DRUGS
USE
1 Abituzomab Cancer
2 Abiciximab Platelet aggregation inhibitor
3 concizumab Bleeding
4 codrituzumab Cancer
5 Crotedumab Diabetes
6 Erenumab Migraine
7 Ibalizumab HIV infection
8 Omalizumab Allergic asthma
9 Sarelumab Rheumatoid arthritis
10 Nesvacumab Cancer
14. CONCLUSION
The study of monoclonal antibodies on this literature review gives a brief idea about
production and application of this very important class of antibody. Monoclonal
antibodies is widely applied in different diagnostic and therapeutic purpose. Regular
research is going on for development of new classes of drug involving monoclonal
antibodies which are widely applied for different acute and chronic diseases, apart
from clinical diagnostic application even diseases like cancer and other fatal
disorders are now a days treated with monoclonal antibodies.
15. REFERENCES
[1]Satyanarayana U, Biotechnology, Uppala author-publisher interlinks, 2011, 213-226.
[2]Shah Jignesh, Panchal Jignasha, ,Monoclonal antibodies present new opportunities in
disease diagnosis and treatment, Asian journal of pharmaceutical technology and
innovation, 04(19), 2016, 51-68.
[3]Ansar Waliza, Ghosh Shyamasree, Monoclonal antibodies a tool in clinical research.
Indian journal of clinical medicine, 4, 2013, 9-21
[4]Kitson I Sean, Application of radionuclide's and antibody drug conjugates to targets
cancer, Cancer studies and molecular medicine, 1(1), 2014, 1-7
[5]Mahroof Shafras, Pant Greetika and Rafeek Rukshan, Monoclonal antibodies an
emerging immunotherapy technology, European journal of biomedical and
pharmaceutical sciences, 3(4), 2016, 134-143
[6]Yucel Fatima, Cirakoglu Beyazit, Production of monoclonal antibodies specific for
progesterone, Tr.j.of biology, 23, 1999, 393-399