This document provides information on spirochetes, including their morphology, classification, pathogenic species, and details on specific diseases caused by them. It discusses the characteristics of Treponema pallidum and Borrelia burgdorferi, which cause syphilis and Lyme disease respectively. It also provides clinical manifestations, laboratory diagnosis, treatment and other relevant details about these diseases. Leptospira interrogans is described as the causative agent of Weil's disease or leptospirosis. The document contains detailed diagrams and explanations.
Haemophilus is the name of a group of bacteria. There are several types of Haemophilus. They can cause different types of illnesses involving breathing, bones and joints, and the nervous system. One common type, Hib (Haemophilus influenzae type b), causes serious disease. It usually strikes children under 5 years old
Haemophilus is the name of a group of bacteria. There are several types of Haemophilus. They can cause different types of illnesses involving breathing, bones and joints, and the nervous system. One common type, Hib (Haemophilus influenzae type b), causes serious disease. It usually strikes children under 5 years old
Clostridium are anerobic gram positive rod shaped spore forming organisms responsible to cause various life threatening diseases in humans like Gas gangrene, Tetanus, Botulism, etc
This presentation contains information about Bacterial Taxonomy, techniques of bacterial classification (Classical and Molecular characteristics) and Bergey's Manual
Clostridium are anerobic gram positive rod shaped spore forming organisms responsible to cause various life threatening diseases in humans like Gas gangrene, Tetanus, Botulism, etc
This presentation contains information about Bacterial Taxonomy, techniques of bacterial classification (Classical and Molecular characteristics) and Bergey's Manual
Treponema is a genus of spiral-shaped bacteria. The major treponeme species of human pathogens is Treponema pallidum, whose subspecies are responsible for diseases such as syphilis, bejel, and yaws.
Infections and salivary gland disease in pediatric age: how to manage - Slide...WAidid
The slideset by Professor Susanna Esposito aims at explaining how to manage the salivary gland infections in pediatric age, from pathogenesis, to transmission, treatments and vaccination coverage, that should be urgently increased in Italy as well as in EU Countries.
Urticariform Lesions as a Manifestation of Secondary Syphilis: Case Reportsemualkaira
In recent years, syphilis has grown again with alarming numbers,
not only in Brazil, but worldwide. In October 2016, the Ministry
of Health recognized that the situation was getting out of hand and
declared an epidemic.
Most cases are in the Southeast region (56%), the most urbanized
and developed in the country. The disease that previously affected
the poorest population, today affects all social classes
Urticariform Lesions as a Manifestation of Secondary Syphilis: Case Reportsemualkaira
In recent years, syphilis has grown again with alarming numbers, not only in Brazil, but worldwide. In October 2016, the Ministry of Health recognized that the situation was getting out of hand and declared an epidemic.
Syphilis is a sexually transmitted infection caused by the bacterium Treponema pallidum subspecies pallidum. The signs and symptoms of syphilis vary depending in which of the four stages it presents (primary, secondary, latent, and tertiary).
The prostate is an exocrine gland of the male mammalian reproductive system
It is a walnut-sized gland that forms part of the male reproductive system and is located in front of the rectum and just below the urinary bladder
Function is to store and secrete a clear, slightly alkaline fluid that constitutes 10-30% of the volume of the seminal fluid that along with the spermatozoa, constitutes semen
A healthy human prostate measures (4cm-vertical, by 3cm-horizontal, 2cm ant-post ).
It surrounds the urethra just below the urinary bladder. It has anterior, median, posterior and two lateral lobes
It’s work is regulated by androgens which are responsible for male sex characteristics
Generalised disease of the prostate due to hormonal derangement which leads to non malignant enlargement of the gland (increase in the number of epithelial cells and stromal tissue)to cause compression of the urethra leading to symptoms (LUTS
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Ve...kevinkariuki227
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Verified Chapters 1 - 19, Complete Newest Version.pdf
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Verified Chapters 1 - 19, Complete Newest Version.pdf
Tom Selleck Health: A Comprehensive Look at the Iconic Actor’s Wellness Journeygreendigital
Tom Selleck, an enduring figure in Hollywood. has captivated audiences for decades with his rugged charm, iconic moustache. and memorable roles in television and film. From his breakout role as Thomas Magnum in Magnum P.I. to his current portrayal of Frank Reagan in Blue Bloods. Selleck's career has spanned over 50 years. But beyond his professional achievements. fans have often been curious about Tom Selleck Health. especially as he has aged in the public eye.
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Introduction
Many have been interested in Tom Selleck health. not only because of his enduring presence on screen but also because of the challenges. and lifestyle choices he has faced and made over the years. This article delves into the various aspects of Tom Selleck health. exploring his fitness regimen, diet, mental health. and the challenges he has encountered as he ages. We'll look at how he maintains his well-being. the health issues he has faced, and his approach to ageing .
Early Life and Career
Childhood and Athletic Beginnings
Tom Selleck was born on January 29, 1945, in Detroit, Michigan, and grew up in Sherman Oaks, California. From an early age, he was involved in sports, particularly basketball. which played a significant role in his physical development. His athletic pursuits continued into college. where he attended the University of Southern California (USC) on a basketball scholarship. This early involvement in sports laid a strong foundation for his physical health and disciplined lifestyle.
Transition to Acting
Selleck's transition from an athlete to an actor came with its physical demands. His first significant role in "Magnum P.I." required him to perform various stunts and maintain a fit appearance. This role, which he played from 1980 to 1988. necessitated a rigorous fitness routine to meet the show's demands. setting the stage for his long-term commitment to health and wellness.
Fitness Regimen
Workout Routine
Tom Selleck health and fitness regimen has evolved. adapting to his changing roles and age. During his "Magnum, P.I." days. Selleck's workouts were intense and focused on building and maintaining muscle mass. His routine included weightlifting, cardiovascular exercises. and specific training for the stunts he performed on the show.
Selleck adjusted his fitness routine as he aged to suit his body's needs. Today, his workouts focus on maintaining flexibility, strength, and cardiovascular health. He incorporates low-impact exercises such as swimming, walking, and light weightlifting. This balanced approach helps him stay fit without putting undue strain on his joints and muscles.
Importance of Flexibility and Mobility
In recent years, Selleck has emphasized the importance of flexibility and mobility in his fitness regimen. Understanding the natural decline in muscle mass and joint flexibility with age. he includes stretching and yoga in his routine. These practices help prevent injuries, improve posture, and maintain mobilit
Report Back from SGO 2024: What’s the Latest in Cervical Cancer?bkling
Are you curious about what’s new in cervical cancer research or unsure what the findings mean? Join Dr. Emily Ko, a gynecologic oncologist at Penn Medicine, to learn about the latest updates from the Society of Gynecologic Oncology (SGO) 2024 Annual Meeting on Women’s Cancer. Dr. Ko will discuss what the research presented at the conference means for you and answer your questions about the new developments.
- Video recording of this lecture in English language: https://youtu.be/lK81BzxMqdo
- Video recording of this lecture in Arabic language: https://youtu.be/Ve4P0COk9OI
- Link to download the book free: https://nephrotube.blogspot.com/p/nephrotube-nephrology-books.html
- Link to NephroTube website: www.NephroTube.com
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New Drug Discovery and Development .....NEHA GUPTA
The "New Drug Discovery and Development" process involves the identification, design, testing, and manufacturing of novel pharmaceutical compounds with the aim of introducing new and improved treatments for various medical conditions. This comprehensive endeavor encompasses various stages, including target identification, preclinical studies, clinical trials, regulatory approval, and post-market surveillance. It involves multidisciplinary collaboration among scientists, researchers, clinicians, regulatory experts, and pharmaceutical companies to bring innovative therapies to market and address unmet medical needs.
Title: Sense of Taste
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the structure and function of taste buds.
Describe the relationship between the taste threshold and taste index of common substances.
Explain the chemical basis and signal transduction of taste perception for each type of primary taste sensation.
Recognize different abnormalities of taste perception and their causes.
Key Topics:
Significance of Taste Sensation:
Differentiation between pleasant and harmful food
Influence on behavior
Selection of food based on metabolic needs
Receptors of Taste:
Taste buds on the tongue
Influence of sense of smell, texture of food, and pain stimulation (e.g., by pepper)
Primary and Secondary Taste Sensations:
Primary taste sensations: Sweet, Sour, Salty, Bitter, Umami
Chemical basis and signal transduction mechanisms for each taste
Taste Threshold and Index:
Taste threshold values for Sweet (sucrose), Salty (NaCl), Sour (HCl), and Bitter (Quinine)
Taste index relationship: Inversely proportional to taste threshold
Taste Blindness:
Inability to taste certain substances, particularly thiourea compounds
Example: Phenylthiocarbamide
Structure and Function of Taste Buds:
Composition: Epithelial cells, Sustentacular/Supporting cells, Taste cells, Basal cells
Features: Taste pores, Taste hairs/microvilli, and Taste nerve fibers
Location of Taste Buds:
Found in papillae of the tongue (Fungiform, Circumvallate, Foliate)
Also present on the palate, tonsillar pillars, epiglottis, and proximal esophagus
Mechanism of Taste Stimulation:
Interaction of taste substances with receptors on microvilli
Signal transduction pathways for Umami, Sweet, Bitter, Sour, and Salty tastes
Taste Sensitivity and Adaptation:
Decrease in sensitivity with age
Rapid adaptation of taste sensation
Role of Saliva in Taste:
Dissolution of tastants to reach receptors
Washing away the stimulus
Taste Preferences and Aversions:
Mechanisms behind taste preference and aversion
Influence of receptors and neural pathways
Impact of Sensory Nerve Damage:
Degeneration of taste buds if the sensory nerve fiber is cut
Abnormalities of Taste Detection:
Conditions: Ageusia, Hypogeusia, Dysgeusia (parageusia)
Causes: Nerve damage, neurological disorders, infections, poor oral hygiene, adverse drug effects, deficiencies, aging, tobacco use, altered neurotransmitter levels
Neurotransmitters and Taste Threshold:
Effects of serotonin (5-HT) and norepinephrine (NE) on taste sensitivity
Supertasters:
25% of the population with heightened sensitivity to taste, especially bitterness
Increased number of fungiform papillae
micro teaching on communication m.sc nursing.pdfAnurag Sharma
Microteaching is a unique model of practice teaching. It is a viable instrument for the. desired change in the teaching behavior or the behavior potential which, in specified types of real. classroom situations, tends to facilitate the achievement of specified types of objectives.
2. BACKGROUND
◦ Gram negative, thin, flexible, elongated, motile,
spirally coiled helical bacilli.
◦ Speira, meaning coil
◦ Chaite, meaning hair
◦ However their cell wall differs from other GNB by
presence of endoflagella.
◦ Endoflagella do not protrude outside, but present
in the periplasmic space between peptidoglycan
layer and outer membrane
◦ These are responsible for various motility of
spirochetes such as:
◦ Flexion-extension type
◦ Corkscrew type rotatory movement
◦ Soft bending at right angle to mid point
3. CLASSIFICATION
◦ Most of the spirochetes are saprophytes.
◦ Only 3 of them are major human
pathogens
◦ Treponema
◦ Borrelia
◦ Leptospira
◦ The pathogenic spirochetes their mode of
transmission and spectrum of diseases
produced.
◦ They also differ in their morphological
properties.
4. MORPHOLOGY
◦ Treponema measure 6-14 um in length; posses 6-12 number of spirals. They
causes Syphilis, Endemic syphilis, Yaws, Pinta.
◦ Borrelia are larger in size (10-30 um in length), posses 3-10 number of spirals.
They cause various diseases such as relapsing fever, Lyme disease and Vincent's
angina.
◦ Leptospira measure 6-20 um in length. They posses numerous, tightly coiled
spirals and with hooked ends. They cause Weil's disease.
5. TREPONEMA
◦ Trepos means “To turn” ; Nema means “Thread”
◦ Thus these are the slender spirochetes with fine spirals and pointed end.
◦ Can’t grow in vitro (Culture media). [Exception of Koch’s Postulates].
◦ T.pallidum causes a sexually transmitted disease, called Syphilis
◦ Nonvenereal treponematoses are transmitted by nonsexual mode (direct
contact) and cause mainly cutaneous lesions.
◦ T.pertenue - Yaws
◦ T. endemicum – Endemic syphilis
◦ T.carateum - Pinta
6. SYPHILIS
◦ One of the oldest sexually transmitted disease (STD) known science 15th century.
◦ Caused by Treponema pallidum.
◦ Incubation period – 10 to 90days.
◦ Mode of transmission
◦ Sexual contact
◦ Direct contact
◦ Blood transfusion
◦ Transplacental – Congenital syphilis
7. CLINICAL MANIFESTATION
◦ Approximately 30% of people developed disease who sexually exposed to infective
partner.
◦ Patients suffer from this disease pass through 4 stages if left un treated:
Primary
Secondary
Latent
Tertiary (Late)
8. Primary Syphilis
◦ Primary (or hard) chancre: Singe painless hard
indurated ulcer; covered by thick exudate rich in
spirochetes. The most common sites are penis (Fig:
A), cervix or labia (Fig: F), and anal canal, rectum or
mouth (Fig: B,D,E).
◦ If acquired by non-venereal mode, then the primary
syphilis is presented as follows:
◦ Transmitted by direct contact, the primary chancre
is extragenital, usually on the fingers. (Fig: C)
◦ Transmitted by blood transfusion then chancre
does not occur
9. ◦ The chancre generally heals within 4-6 weeks (range 2-12 weeks), but lymphadenopathy
may persist for months.
◦ Regional (usually inguinal) lymphadenopathy appears within 1 week of onset of skin
lesions.
◦ Lymph nodes are painless firm, non-suppurative, and often bilateral
10. Secondary Syphilis
◦ Usually develops 6-12 weeks after the healing of primary lesion.
◦ Skin rashes (palms and soles)
◦ Condylomata lata: Mucocutaneous papules which coalesce to form large pink to grey
lesions in warm moist intertriginous areas such as perianal region, vulva, and scrotum.
◦ Mucous patches (superficial mucosal erosions)
11. Latent Syphilis
◦ Secondary lesions usually subside within 2-6 weeks, and the infection proceeds to latent
syphilis; which is characterized by absence of clinical manifestations of syphilis with
positive serological tests for syphilis.
◦ Latent syphilis may be early latent syphilis (occurs within first year after infection) and
late latent syphilis (occurs after the first year of infection)
◦ Patients are still infectious transmitting the infection either by bloodstream or in utero.
◦ Latent syphilis may have one of the following fates:
◦ Persistent lifelong infection (common)
◦ Development of late syphilis (rare)
◦ Spontaneous cure.
12. Late Syphilis
◦ Several decades after the initial infection, about one-third of untreated patients
develop tertiary syphilis.
◦ 5% develop gummatous lesions
◦ 10% develop cardiovascular lesions
◦ Remaining 10% develop neurosyphilis.
13. STAGE SYMPTOMS TIMELINE
Primary Painless sores called
chancre at the site of
inoculation or contact
of the pathogen
Begins from 3 or 4 weeks after
exposure
Secondary Rash and other systemic
symptoms
Begins from 4 to 8 weeks from
the onset of primary symptoms
Latent Asymptomatic Early and late latency period
varying about a year
Late Starting from benign
lesions, can damage the
cardiovascular and
central nervous system
Mostly after 8 to 10 years
14. LABORATORY DIAGNOSIS
◦ Microscopy
◦ Dark ground microscopy
◦ Direct IF staining for T. pallidum (DFA-TP)
◦ Silver impregnation method
◦ Culture: Not cultivable, maintained in rabbit testes
◦ Serology: (Antibody detection)
◦ Standard tests for syphilis: (Cardiolipin antigen)
◦ VDRL (Venereal disease research laboratory) test
◦ RPR (Rapid plasma reagin)
◦ TRUST (toluidine red unheated serum test)
◦ USR (Unheated serum reagin test)
◦ Specific test: (T.pallidum antigen)
◦ TPI (T.pallidum immobilization test)
◦ FTA-ABS (Fluorescent treponemal antibody absorption test)
◦ TPA (T.pallidum agglutination test)
◦ TPHA (T.pallidum hemagglutination test)
◦ TPPA (T pallidum particle agglutination test)
◦ Polymerase chain reaction (PCR)
15. VDRL
Principle: Precipitation (Slide flocculation test)
Procedure: 50 uL of patient's serum (heat inactivated) is
mixed with a drop of VDRL antigen on a concave slide,
which is then mixed by rotating the slide for 4 minutes
Result: Positive test (i.e. reactive) is indicated by formation
of medium to large clumps of antigen antibody
complexes; visualized by focusing the slide under
microscope (10x).
Uses:
◦ Cheaper
◦ Preferred as a screening test (e.g. antenatal screening)
◦ Detect antibody in CSF (Neurosyphilis)
◦ Monitor treatment response
16. RPR
◦ Rapid Plasma Reagin (RPR) RPR is another slide
flocculation test using disposable plastic cards
having clearly defined circles.
◦ It is similar to VDRL test with some differences
such as:
◦ RPR antigen has a prolonged shelf-life, therefore it
is preferred to test individual sample (less sample
load);
◦ Results can be read in naked eyes, without the need
of a microscope, as the clumps formed are bigger
in size
◦ It can only be used for detecting antibodies in blood
not in CSF
◦ It is more expensive than VDRL.
17. ADVANTAGES & DISADVANTAGES OF STANDARD
TESTS
ADVANTAGES
◦ Monitor the to the response to treatment
◦ Detect Neurosyphilis
◦ Reagin antibody becomes detectable 7-10 days after the appearance of primary chancre (or 3-5 weeks
after acquiring the infection)
◦ The sensitivity of nontreponemal tests varies from 78 to 85% in primary stage, 100% in secondary
stage, 71-73% in late stage and the specificity is around 98-99%.
DISADVANTAGES
◦ Biological false-positive (BFP) reactions: The incidence of BFP is generally 1-2%. This is because
reagin antibodies may also be found in patients with unrelated diseases such as lepromatous leprosy,
relapsing fever, malaria, viral hepatitis, HIV, pregnancy and IV drug abusers
◦ Prozone phenomena: If antibody titer in patient's sera is high, it may lead to false negative result hence
it is essential to test sera in dilutions
◦ Sensitivity of non-treponemal tests is low in late stage of syphilis.
18. TREATMENT
◦ Penicillin is the drug of choice for all the stages of syphilis
◦ Alternative drug is used in patients with penicillin allergy: Tetracycline is recommended
EVALUATION AFTER TREATMENT
◦ Non-treponemal test, such as VDRIL and RPR are preferred.
◦ VDRL has to be done at 3 months' intervals for at least 1 year.
PREVENTION
◦ Treatment of cases and contacts (sexual partners)
◦ Education about safe sex practices
◦ Prophylactic use of barrier contraceptive methods.
19. CONGENITAL SYPHILIS
◦ May Occur at any stage of pregnancy, but fetal damage occurs only after fourth month of gestation.
◦ Untreated cases of early maternal syphilis are at higher risk.
◦ Antenatal screening and treatment of positive cases during pregnancy may prevent congenital syphilis.
◦ Earliest manifestations : Occur within 2 years of age and is infectious
◦ They suffer from rhinitis, mucocutaneous lesions, bone changes, hepatosplenomegaly and
lymphadenopathy.
◦ Late congenital syphilis: Occurs after 2 years and is non-infectious.
◦ It is characterized by interstitial keratitis, eighth- nerve deafness, bilateral knee effusions.
◦ Residual stigmata may remain for long time such as:
◦ Hutchinson's teeth (notched central incisors)
◦ Mulberry molars (molars with poorly developed cusps)
◦ Saddle nose
◦ Saber shins.
21. BORRELIOSIS
◦ Borrelia are larger in size (10-30 um in length), posses 3-10 number of spirals.
◦ Most of the species are commensals on the buccal and genital mucosa.
◦ Few are pathogenic to human
◦ B. recurrentis - Relapsing fever
◦ B. burgdorferia - Lyme disease
◦ B. vincentii - Vincent's angina.
22. Relapsing Fever
CHARACTERISTIC EPIDEMIC RF ENDEMIC RF
CASETIVE AGENT B.recurrentis B.duttonii, B.hermsii, B.turicatae
MODE OF TERANSMITION Louse Ticks
ROUT OF ENTRY Crushing or Scratching Bite
C/F Recurrent febrile episode (Fever: 3-4days;Afebrile: 4-14days), Petechia,
Some neurological feature (Seizure, Paraplegia, Meningitis, Psychosis etc.
)
LAB DIAGONOSIS Microscopy, Smear (Giemsa stain), Culture, Serology (ELISA,IFA etc.)
TREATMENT Doxycycline or Erythromycin
RF characteristic by recurrent episodes of fever and nonspecific symptoms following
exposure to insect vector carrying Borrelia species.
23. Lyme Disease
CHARACTERISTIC SUMMERY
OTHER NAME Lyme borreliosis
AGENT Borrelia burgdorferi
RESERVOIRS Rodent & Deer
TRANSMITTED BY Tick
CLINICAL MANIFESTATIONS (3 Stages) • Early localized infection: After an incubation period of 3-32 days, an
annular maculopapular lesion develops at the site of the tick bite
called erythema migrans, commonly involving thigh and groin.
• Early disseminated infection: Spreads hematogenously, resulting in
secondary annular skin lesions, arthralgia, malaise and neurological
abnormalities.
• Late persistent infection (Lyme arthritis): About 60% patients
develop arthritis of large joints (e.g. knees), lasting for months.
LABORATORY DIAGNOSIS Culture, Molecular methods, Serology
TREATMENT Doxycycline (Adult), Amoxicillin (Children)
24. Vincent's Angina
CHARACTERISTIC SUMMERY
DEFINITION Acute necrotizing ulcerative gingivitis
OTHER NAME Trench mouth or Vincent's stomatitis
AGENT Borrelia vincentii
CLINICAL MANIFESTATIONS • Gingivitis with sudden onset of painful bleeding gums
• Foul breath
• Bad taste
• Gingival mucosa becomes ulcerated and may be covered by gray
"pseudomembrane" resembling diphtheria, but peels off easily
LABORATORY DIAGNOSIS Microscopic smear
TREATMENT Treatment consists of debridement and antibiotics(Metronidazole
plus Penicillin).
PROGNOSIS Mortality is high if not treated
25. LEPTOSPIRA
◦ Leptospira is antigenically complex; comprises of two species:
◦ L. interrogans: Pathogenic to man.
◦ L. biflexa: Saprophyte and not pathogenic to man.
26. Leptospirosis
◦ Leptospira interrogans causes leptospirosis or Weil's disease
◦ It is a zoonotic disease
MODE OF TRANSMISSION: Direct human-to-human transmission does not occur.
◦ Indirect contact with water, moist soil and wet surfaces contaminated with animal urine or
◦ Direct contact with urine and products of parturition, placenta of infected animals.
SOURCE: Rats, dogs, cattle and pigs.
SEASONALITY: Leptospirosis is more common in rainy and post monsoon period.
27. RISK FACTORS: Lower socioeconomic status
◦ Urban and rural slum areas
◦ Rainfall and floods
◦ Occupational exposure: Agricultural workers (e.g. rice field planters),
fishermen, sewer workers
3R’s: Rodents
Rainfall
Rice field Incidence
28. Pathogenesis
There are two distinct phases of pathogenesis following leptospiral infection:
First phase (septicemic phase):
◦ After entering through the mucosa (conjunctival or oral) or abraded skin, L. interrogans
spill over to the bloodstream and then disseminate hematogenously to various organs
including brain, liver, lung, heart and kidney.
◦ Vascular damage: Spirochetes can invasion of tissues ,walls of capillaries and vessels due
to active motility and release of hyaluronidase.
Second phase (immune phase):
◦ As antibodies develop, spirochetes disappear from the blood.
◦ Antigen antibody complexes are deposited in various organs
◦ Renal colonization: Bacilli become adherent to the proximal renal tubular brush border
and are excreted in urine.
29. Clinical Manifestations
The incubation period is around 10 (1 to 30) days. In general, the manifestations can be
divided into two distinct clinical syndromes
1. Mild anicteric febrile illness
2. Weil's disease (Hepato-renal-hemorrhagic syndrome)
30. Laboratory diagnosis
SPECIMENS: CSF, Blood and Urine
MICROSCOPY
Dark ground microscope or silver impregnation staining
ISOLATION:
◦ Culture condition: 30°C for 4-6 weeks
◦ Medium: EMJH medium, Korthof's and Fletcher's media.
SEROLOGY FOR ANTIBODY DETECTION
◦ Genus specific tests: Macroscopic slide agglutination test, Latex
agglutination test, ELISA, ICT
◦ Serovar specific test: Microscopic agglutination test
MOLECULAR METHODS: PCR
NONSPECIFIC FINDINGS: Altered LFT, RFT
◦
31. TREATMENT
◦ Mild leptospirosis should be treated with oral doxycycline (100 mg twice a day for 7 days).
◦ Amoxicillin can be given alternatively
◦ Severe leptospirosis: Penicillin is the drug of choice (1.5 million units IV, four times a day for 7 days)
◦ Alternatives being ceftriaxone or cefotaxime.
PREVENTION
Vaccine
◦ SPIROLEPT manufactured by Sanofi-Pasteur is available for subcutaneous injection as two doses at 15 days
interval, with the third dose 4-6 months after the first dose, followed by biannual revaccination.
General Measures
◦ Chemoprophylaxis with doxycycline is recommended for high risk individual
◦ Rodent control
◦ Avoidance of swimming in contaminated places
◦ Health education