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Principles of Vascular 
Anastomosis 
By 
Professor 
Abdulsalam Y Taha 
School of Medicine/ University of Sulaimaniyah/ Region of 
Kurdistan/Iraq 
https://sulaimaniu.academia.edu/AbdulsalamTaha
Introduction 
 The principles of vascular repair with sutures were established 
in the first decade of the 20th century by Alexis Carrel, who in 
1912 was awarded the Nobel Prize for medicine for his work . 
 Since then, technical refinements of suture materials have 
made possible surgical reconstruction of most arteries from the 
root of the aorta to microvascular anastomosis or repair of the 
smallest vessels, e.g., digital arteries or those on the surface of 
the brain. 
 Fine sutures on atraumatic needles are best for arterial 
anastomosis. 
 Silk was used for many years, but it has now been replaced by 
synthetic fibers, which are less traumatic to the vessel walls. 
Prof. A Y Taha: Principles of 
15/10/14 2 
vascular anastomosis
History 
 1899 – Dorfler advocated use of all layers of vessels 
in repair 
 1907 – (Carrel) “The Surgery of Blood Vessels” (JH 
Hospital Bull.) 
 1st replantation of canine limbs 
 1st esophageal-intestinal interposition 
 1959 – (Seidenberg) human esophageal-intestinal 
interposition 
 1960 – (Jacobson/Suarez) operating microscope 
introduced (1 mm vessels) 
 1966 – (Antia/Buch) fasciocutaneous transfer 
 1972 – (McLean/Buncke) omental flap to scalp 
Prof. A Y Taha: Principles of 
15/10/14 3 
vascular anastomosis
a. Pass a right angle clamp gently through the soft tissue 
directly on the dorsal aspect of the artery and direct it 
away from the larger veins to avoid iatrogenic injuries. 
Caution! Avoid accidental penetration of the dorsal wall of 
the artery. b. Gently lift the artery with the vessel-loop to 
achieve tension in the tissues, thus facilitating the 
dissection. 
Prof. A Y Taha: Principles of 
15/10/14 4 
vascular anastomosis
Different methods for controlling bleeding are demonstrated. 
From left to right: doubly applied vessel loop, bulldog 
( small metallic vascular clamp), balloon catheter, 
loop of ligature, vascular clamp). 
Prof. A Y Taha: Principles of 
15/10/14 5 
vascular anastomosis
√ ᵡ 
Prof. A Y Taha: Principles of 
15/10/14 6 
vascular anastomosis
Prof. A Y Taha: Principles of 
15/10/14 7 
vascular anastomosis
15/10/14 Prof. A Y Taha: Principles of vascular anastomosis 8
Prof. A Y Taha: Principles of 
15/10/14 9 
vascular anastomosis
Prof. A Y Taha: Principles of 
15/10/14 10 
vascular anastomosis
Prof. A Y Taha: Principles of 
15/10/14 11 
vascular anastomosis
Simple suture 
Prof. A Y Taha: Principles of 
15/10/14 12 
vascular anastomosis
Kunlin suture 
● If an endarterectomy has been performed, 
there is a risk of intimal flap dissection at 
the downstream edge. To eleminate this 
risk, sutures are inserted to secure the 
intima. The needle passes from outside to 
inside through an endarterectomized part 
of the wall and back from inside to outside 
through the atheroma to be finally tied on 
the outside. 
Prof. A Y Taha: Principles of 
15/10/14 13 
vascular anastomosis
Prof. A Y Taha: Principles of 
15/10/14 14 
vascular anastomosis
Patch angioplasty 
Prof. A Y Taha: Principles of 
15/10/14 15 
vascular anastomosis
End to end anastomosis: stay 
sutures 
Prof. A Y Taha: Principles of 
15/10/14 16 
vascular anastomosis
End to end anastomosis: 
interrupted suture 
Prof. A Y Taha: Principles of 
15/10/14 17 
vascular anastomosis
End to end anastomosis: 
continuous suture 
Prof. A Y Taha: Principles of 
15/10/14 18 
vascular anastomosis
When two vessels with different 
diameters are being sutured 
end to end, the smaller has to 
be slit open and the edges 
trimmed to fit the larger one, 
which must be cut somewhat 
obliquely to avoid kinking. 
Prof. A Y Taha: Principles of 
15/10/14 19 
vascular anastomosis
Prof. A Y Taha: Principles of 
15/10/14 20 
vascular anastomosis
End to end anastomosis: 
single-stitch method 
● Used when there is 
a difficulty in rotating the 
vessels, for example at 
a large bifurcation. 
● Commensing on the side 
nearest the operater, the 
sutures are inserted from 
within the lumen to 
complete the deep or 
posterior aspect and then 
continued across the anterior 
aspect to the starting point. 
● Alternatively, a double ended 
suture may be commensed 
at the midpoint posteriorly and 
each side completed in turn. 
Prof. A Y Taha: Principles of 
15/10/14 21 
vascular anastomosis
End to end anastomosis: inlay 
technique 
● Used for AAA repair. 
● Double ended horizontal mattress 
suture in the middle of the graft. 
● Needles should pass from graft to 
aorta 
● Take large bites incorporating all 
layers. 
Prof. A Y Taha: Principles of 
15/10/14 22 
vascular anastomosis
Inlay parachute technique 
● The double ended 
suture is left untied 
in order to allow 
a number of stitches 
to be placed on each 
side before the graft 
is pulled down onto 
the artery. 
Prof. A Y Taha: Principles of 
15/10/14 23 
vascular anastomosis
Buttressing sutures 
● Sutures may be buttressed 
with Dacron pieces when 
the wall of the artery is 
friable and may cut out 
causing hemorrhage. 
Prof. A Y Taha: Principles of 
15/10/14 24 
vascular anastomosis
End to side anastomosis: four 
quadrant technique 
Prof. A Y Taha: Principles of 
15/10/14 25 
vascular anastomosis
End to side anastomosis: 
parachute technique 
Prof. A Y Taha: Principles of 
15/10/14 26 
vascular anastomosis
Prof. A Y Taha: Principles of 
15/10/14 27 
vascular anastomosis
How to make a venous patch? 
Prof. A Y Taha: Principles of 
15/10/14 28 
vascular anastomosis
Spiral graft technique 
 Spiral graft 
technique to create a 
graft of large 
diameter for 
replacing vein 
segments. A 
saphenous vein is 
cut longituidinally 
and sutured in a 
spiral fashion over 
plastic tubing used 
as a stent. 
Prof. A Y Taha: Principles of 
15/10/14 29 
vascular anastomosis
Prof. A Y Taha: Principles of 
15/10/14 30 
vascular anastomosis
Non- sutured anastomosis 
Prof. A Y Taha: Principles of 
15/10/14 31 
vascular anastomosis
Microvascular surgical technique 
 Trim adventitia 
 2-3mm 
 Gentle handling (no full-thickness) 
 Trim free edge, if needed 
 Dissect vessels from 
surrounding tissues 
 Irrigate and dilate 
 Heparinized saline 
 Mechanical dilation (1 ½ 
times normal –paralyses 
smooth muscle) 
 Chemical dilation, if 
necessary 
 Suturing 
Prof. A Y Taha: Principles of 
15/10/14 32 
vascular anastomosis
Microvascular suture 
technique 
 3 guide sutures (120 
degrees apart) 
 Perpendicular piercing 
 Entry point 2x thickness of 
vessel from cut end 
 Equal bites on either side 
 Microforceps in lumen vs. 
retracting adventitia 
 Pull needle through in 
circular motion 
 Surgeon’s knot with guide 
sutures, simple for others 
 Avoid backwalling—2 
bites/irrigation 
Prof. A Y Taha: Principles of 
15/10/14 33 
vascular anastomosis
3 suture technique 
Prof. A Y Taha: Principles of 
15/10/14 34 
vascular anastomosis
End-to-side Anastomosis 
Prof. A Y Taha: Principles of 
15/10/14 35 
vascular anastomosis
Mechanical anastomosis 
 Devices 
 Clips 
 Coupler 
 Laser 
 Results 
 Increased efficiency and 
speed, use in difficult areas 
 Patency rates at least equal 
to hand-sewn (Shindo, et al 
1996, De Lorenzi, et al 2002) 
 Can be used for end-to-end 
or end-to-side (DeLacure, et 
al 1999) 
 Poorer outcome with arterial 
anastomosis—20-25% 
failure (Shindo, et al 1996, 
Ahn, et al 1994) 
Prof. A Y Taha: Principles of 
15/10/14 36 
vascular anastomosis
Microvascular Hints & Helps 
 Use background to help 
visualize suture 
 Demagnetize instruments, if 
needed 
 May reclamp vessels for 
repair after 15 minutes of 
flow 
 Reclamp both arterial and 
venous vessels when 
revising venous anastomosis 
 Support your hands and hold 
instruments like a pencil 
Prof. A Y Taha: Principles of 
15/10/14 37 
vascular anastomosis
Mechanical flap monitoring 
 Doppler 
 External 
 Implanted 
 Buried flaps 
 80-100% salvage 
(Disa J, et 
al 1999) 
 Color flow 
 Other 
Prof. A Y Taha: Principles of 
15/10/14 38 
vascular anastomosis
Complications of Vascular 
Anastomosis 
Badr Aljabri MD, FRCSC 
Associate Professor and Consultant 
Vascular Surgeon, KKUH
Anastomotic bleeding 
 Needle hole bleeding. 
- more common with PTFE grafts. 
- Rx: Local haemostatic agents. 
Reverse systemic heparin 
effect. 
Prof. A Y Taha: Principles of 
15/10/14 40 
vascular anastomosis
Anastomotic bleeding 
 Suture line bleeding. 
- Rx: Simple or U-shaped suture at 
the defect. 
tying should be with non- 
Pulsetile flow. 
Prof. A Y Taha: Principles of 
15/10/14 41 
vascular anastomosis
Anastomotic Psudoaneurysm 
 Disruption of the suture line at the 
anastomosis result in walled off extra-luminal 
circulation of the blood. 
Prof. A Y Taha: Principles of 
15/10/14 42 
vascular anastomosis
Patient Factors 
1. Native Artery Disease. 
2. Infection. 
3. Smoking 
4. Hypertension. 
5. Healing complications 
( Seroma, Hematoma) 
Material Factors 
1. Graft Defect 
2. Suture Degradation or 
breakage. 
3. Prosthetic graft- arterial wall 
Technical Factors compliance mismatch 
1. Inadequate suture bites. 
2. Excessive tension. 
3. Joint Motion. 
4. Redo Procedure. 
5. Endarterectomy. 
Prof. A Y Taha: Principles of 
15/10/14 43 
vascular anastomosis
Prof. A Y Taha: Principles of 
15/10/14 44 
vascular anastomosis
Prof. A Y Taha: Principles of 
15/10/14 45 
vascular anastomosis
Prof. A Y Taha: Principles of 
15/10/14 46 
vascular anastomosis
Prof. A Y Taha: Principles of 
15/10/14 47 
vascular anastomosis
Anastomotic stenosis 
 Early : Technical. 
 1-18 months: Intimal hyperplasia. 
 > 18 months: Progression of 
atherosclerosis. 
Prof. A Y Taha: Principles of 
15/10/14 48 
vascular anastomosis
Prof. A Y Taha: Principles of 
15/10/14 49 
vascular anastomosis
Prof. A Y Taha: Principles of 
15/10/14 50 
vascular anastomosis
Prof. A Y Taha: Principles of 
15/10/14 51 
vascular anastomosis
Graft thrombosis 
Early 
1. Technical (kink, missed valve, AV fistula, 
intimal flap) 
2. Poor choice of inflow or outflow sites. 
3. Insufficient runoff. 
4. Ongoing or progression of soft tissue 
infection 
5. Low circulatory volume. 
6. Hypercoagulable state. 
Intermediate 
Intimal Hyperplasia 
(1 month -18 months) 
Late 
1. Progression of Atherosclerosis. 
2. Degenerative lesions in the graft 
Prof. A Y Taha: Principles of 
15/10/14 52 
vascular anastomosis
Thrombectomy 
Prof. A Y Taha: Principles of 
15/10/14 53 
vascular anastomosis
Questions? 
Thanks!!!

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Principles of vascular anastomosis

  • 1. Principles of Vascular Anastomosis By Professor Abdulsalam Y Taha School of Medicine/ University of Sulaimaniyah/ Region of Kurdistan/Iraq https://sulaimaniu.academia.edu/AbdulsalamTaha
  • 2. Introduction  The principles of vascular repair with sutures were established in the first decade of the 20th century by Alexis Carrel, who in 1912 was awarded the Nobel Prize for medicine for his work .  Since then, technical refinements of suture materials have made possible surgical reconstruction of most arteries from the root of the aorta to microvascular anastomosis or repair of the smallest vessels, e.g., digital arteries or those on the surface of the brain.  Fine sutures on atraumatic needles are best for arterial anastomosis.  Silk was used for many years, but it has now been replaced by synthetic fibers, which are less traumatic to the vessel walls. Prof. A Y Taha: Principles of 15/10/14 2 vascular anastomosis
  • 3. History  1899 – Dorfler advocated use of all layers of vessels in repair  1907 – (Carrel) “The Surgery of Blood Vessels” (JH Hospital Bull.)  1st replantation of canine limbs  1st esophageal-intestinal interposition  1959 – (Seidenberg) human esophageal-intestinal interposition  1960 – (Jacobson/Suarez) operating microscope introduced (1 mm vessels)  1966 – (Antia/Buch) fasciocutaneous transfer  1972 – (McLean/Buncke) omental flap to scalp Prof. A Y Taha: Principles of 15/10/14 3 vascular anastomosis
  • 4. a. Pass a right angle clamp gently through the soft tissue directly on the dorsal aspect of the artery and direct it away from the larger veins to avoid iatrogenic injuries. Caution! Avoid accidental penetration of the dorsal wall of the artery. b. Gently lift the artery with the vessel-loop to achieve tension in the tissues, thus facilitating the dissection. Prof. A Y Taha: Principles of 15/10/14 4 vascular anastomosis
  • 5. Different methods for controlling bleeding are demonstrated. From left to right: doubly applied vessel loop, bulldog ( small metallic vascular clamp), balloon catheter, loop of ligature, vascular clamp). Prof. A Y Taha: Principles of 15/10/14 5 vascular anastomosis
  • 6. √ ᵡ Prof. A Y Taha: Principles of 15/10/14 6 vascular anastomosis
  • 7. Prof. A Y Taha: Principles of 15/10/14 7 vascular anastomosis
  • 8. 15/10/14 Prof. A Y Taha: Principles of vascular anastomosis 8
  • 9. Prof. A Y Taha: Principles of 15/10/14 9 vascular anastomosis
  • 10. Prof. A Y Taha: Principles of 15/10/14 10 vascular anastomosis
  • 11. Prof. A Y Taha: Principles of 15/10/14 11 vascular anastomosis
  • 12. Simple suture Prof. A Y Taha: Principles of 15/10/14 12 vascular anastomosis
  • 13. Kunlin suture ● If an endarterectomy has been performed, there is a risk of intimal flap dissection at the downstream edge. To eleminate this risk, sutures are inserted to secure the intima. The needle passes from outside to inside through an endarterectomized part of the wall and back from inside to outside through the atheroma to be finally tied on the outside. Prof. A Y Taha: Principles of 15/10/14 13 vascular anastomosis
  • 14. Prof. A Y Taha: Principles of 15/10/14 14 vascular anastomosis
  • 15. Patch angioplasty Prof. A Y Taha: Principles of 15/10/14 15 vascular anastomosis
  • 16. End to end anastomosis: stay sutures Prof. A Y Taha: Principles of 15/10/14 16 vascular anastomosis
  • 17. End to end anastomosis: interrupted suture Prof. A Y Taha: Principles of 15/10/14 17 vascular anastomosis
  • 18. End to end anastomosis: continuous suture Prof. A Y Taha: Principles of 15/10/14 18 vascular anastomosis
  • 19. When two vessels with different diameters are being sutured end to end, the smaller has to be slit open and the edges trimmed to fit the larger one, which must be cut somewhat obliquely to avoid kinking. Prof. A Y Taha: Principles of 15/10/14 19 vascular anastomosis
  • 20. Prof. A Y Taha: Principles of 15/10/14 20 vascular anastomosis
  • 21. End to end anastomosis: single-stitch method ● Used when there is a difficulty in rotating the vessels, for example at a large bifurcation. ● Commensing on the side nearest the operater, the sutures are inserted from within the lumen to complete the deep or posterior aspect and then continued across the anterior aspect to the starting point. ● Alternatively, a double ended suture may be commensed at the midpoint posteriorly and each side completed in turn. Prof. A Y Taha: Principles of 15/10/14 21 vascular anastomosis
  • 22. End to end anastomosis: inlay technique ● Used for AAA repair. ● Double ended horizontal mattress suture in the middle of the graft. ● Needles should pass from graft to aorta ● Take large bites incorporating all layers. Prof. A Y Taha: Principles of 15/10/14 22 vascular anastomosis
  • 23. Inlay parachute technique ● The double ended suture is left untied in order to allow a number of stitches to be placed on each side before the graft is pulled down onto the artery. Prof. A Y Taha: Principles of 15/10/14 23 vascular anastomosis
  • 24. Buttressing sutures ● Sutures may be buttressed with Dacron pieces when the wall of the artery is friable and may cut out causing hemorrhage. Prof. A Y Taha: Principles of 15/10/14 24 vascular anastomosis
  • 25. End to side anastomosis: four quadrant technique Prof. A Y Taha: Principles of 15/10/14 25 vascular anastomosis
  • 26. End to side anastomosis: parachute technique Prof. A Y Taha: Principles of 15/10/14 26 vascular anastomosis
  • 27. Prof. A Y Taha: Principles of 15/10/14 27 vascular anastomosis
  • 28. How to make a venous patch? Prof. A Y Taha: Principles of 15/10/14 28 vascular anastomosis
  • 29. Spiral graft technique  Spiral graft technique to create a graft of large diameter for replacing vein segments. A saphenous vein is cut longituidinally and sutured in a spiral fashion over plastic tubing used as a stent. Prof. A Y Taha: Principles of 15/10/14 29 vascular anastomosis
  • 30. Prof. A Y Taha: Principles of 15/10/14 30 vascular anastomosis
  • 31. Non- sutured anastomosis Prof. A Y Taha: Principles of 15/10/14 31 vascular anastomosis
  • 32. Microvascular surgical technique  Trim adventitia  2-3mm  Gentle handling (no full-thickness)  Trim free edge, if needed  Dissect vessels from surrounding tissues  Irrigate and dilate  Heparinized saline  Mechanical dilation (1 ½ times normal –paralyses smooth muscle)  Chemical dilation, if necessary  Suturing Prof. A Y Taha: Principles of 15/10/14 32 vascular anastomosis
  • 33. Microvascular suture technique  3 guide sutures (120 degrees apart)  Perpendicular piercing  Entry point 2x thickness of vessel from cut end  Equal bites on either side  Microforceps in lumen vs. retracting adventitia  Pull needle through in circular motion  Surgeon’s knot with guide sutures, simple for others  Avoid backwalling—2 bites/irrigation Prof. A Y Taha: Principles of 15/10/14 33 vascular anastomosis
  • 34. 3 suture technique Prof. A Y Taha: Principles of 15/10/14 34 vascular anastomosis
  • 35. End-to-side Anastomosis Prof. A Y Taha: Principles of 15/10/14 35 vascular anastomosis
  • 36. Mechanical anastomosis  Devices  Clips  Coupler  Laser  Results  Increased efficiency and speed, use in difficult areas  Patency rates at least equal to hand-sewn (Shindo, et al 1996, De Lorenzi, et al 2002)  Can be used for end-to-end or end-to-side (DeLacure, et al 1999)  Poorer outcome with arterial anastomosis—20-25% failure (Shindo, et al 1996, Ahn, et al 1994) Prof. A Y Taha: Principles of 15/10/14 36 vascular anastomosis
  • 37. Microvascular Hints & Helps  Use background to help visualize suture  Demagnetize instruments, if needed  May reclamp vessels for repair after 15 minutes of flow  Reclamp both arterial and venous vessels when revising venous anastomosis  Support your hands and hold instruments like a pencil Prof. A Y Taha: Principles of 15/10/14 37 vascular anastomosis
  • 38. Mechanical flap monitoring  Doppler  External  Implanted  Buried flaps  80-100% salvage (Disa J, et al 1999)  Color flow  Other Prof. A Y Taha: Principles of 15/10/14 38 vascular anastomosis
  • 39. Complications of Vascular Anastomosis Badr Aljabri MD, FRCSC Associate Professor and Consultant Vascular Surgeon, KKUH
  • 40. Anastomotic bleeding  Needle hole bleeding. - more common with PTFE grafts. - Rx: Local haemostatic agents. Reverse systemic heparin effect. Prof. A Y Taha: Principles of 15/10/14 40 vascular anastomosis
  • 41. Anastomotic bleeding  Suture line bleeding. - Rx: Simple or U-shaped suture at the defect. tying should be with non- Pulsetile flow. Prof. A Y Taha: Principles of 15/10/14 41 vascular anastomosis
  • 42. Anastomotic Psudoaneurysm  Disruption of the suture line at the anastomosis result in walled off extra-luminal circulation of the blood. Prof. A Y Taha: Principles of 15/10/14 42 vascular anastomosis
  • 43. Patient Factors 1. Native Artery Disease. 2. Infection. 3. Smoking 4. Hypertension. 5. Healing complications ( Seroma, Hematoma) Material Factors 1. Graft Defect 2. Suture Degradation or breakage. 3. Prosthetic graft- arterial wall Technical Factors compliance mismatch 1. Inadequate suture bites. 2. Excessive tension. 3. Joint Motion. 4. Redo Procedure. 5. Endarterectomy. Prof. A Y Taha: Principles of 15/10/14 43 vascular anastomosis
  • 44. Prof. A Y Taha: Principles of 15/10/14 44 vascular anastomosis
  • 45. Prof. A Y Taha: Principles of 15/10/14 45 vascular anastomosis
  • 46. Prof. A Y Taha: Principles of 15/10/14 46 vascular anastomosis
  • 47. Prof. A Y Taha: Principles of 15/10/14 47 vascular anastomosis
  • 48. Anastomotic stenosis  Early : Technical.  1-18 months: Intimal hyperplasia.  > 18 months: Progression of atherosclerosis. Prof. A Y Taha: Principles of 15/10/14 48 vascular anastomosis
  • 49. Prof. A Y Taha: Principles of 15/10/14 49 vascular anastomosis
  • 50. Prof. A Y Taha: Principles of 15/10/14 50 vascular anastomosis
  • 51. Prof. A Y Taha: Principles of 15/10/14 51 vascular anastomosis
  • 52. Graft thrombosis Early 1. Technical (kink, missed valve, AV fistula, intimal flap) 2. Poor choice of inflow or outflow sites. 3. Insufficient runoff. 4. Ongoing or progression of soft tissue infection 5. Low circulatory volume. 6. Hypercoagulable state. Intermediate Intimal Hyperplasia (1 month -18 months) Late 1. Progression of Atherosclerosis. 2. Degenerative lesions in the graft Prof. A Y Taha: Principles of 15/10/14 52 vascular anastomosis
  • 53. Thrombectomy Prof. A Y Taha: Principles of 15/10/14 53 vascular anastomosis