This document provides an introduction to microscopes. It discusses the history of microscopes beginning with Anton van Leeuwenhoek in the 16th century being the first to observe microorganisms. It then describes the basic parts of a classical/light microscope including the ocular lens, stage, objectives, condenser, and illuminator. It also discusses magnification, resolution, working distance, and different types of microscopy including bright field, dark field, phase contrast, and fluorescence microscopes. The document explains how light interacts with lenses and specimens to produce microscope images.
during this ppt of microscopes we will be able to know
INTRODUCTION
DEFINITION
HISTORICAL BACKGROUND
VARIABLES USED IN MICROSCOPY
VARIOUS TYPES OF MICROSCOPES
COMPOUND MICROSCOPE - Structure and Function
USE OF MICROSCOPE
CARE OF MICROSCOPE
defintion
A microscope (Greek: micron = small and scopos = aim)
MICROSCOPE - An instrument for viewing objects that are too small to be seen by the naked or unaided eye
MICROSCOPY - The science of investigating small objects using such an instrument is called microscopy
during this ppt of microscopes we will be able to know
INTRODUCTION
DEFINITION
HISTORICAL BACKGROUND
VARIABLES USED IN MICROSCOPY
VARIOUS TYPES OF MICROSCOPES
COMPOUND MICROSCOPE - Structure and Function
USE OF MICROSCOPE
CARE OF MICROSCOPE
defintion
A microscope (Greek: micron = small and scopos = aim)
MICROSCOPE - An instrument for viewing objects that are too small to be seen by the naked or unaided eye
MICROSCOPY - The science of investigating small objects using such an instrument is called microscopy
Introduction
History
Compound microscope
Variants of microscopes
Dark field microscope
Phase contrast microscope
Fluorescent microscope
Polarising microscope
Electron microscope
A Microscope is an instrument for viewing objects that are too small to be seen by the naked/ unaided eyes.
In Greek micron= small
skopien=to look at
The science of investigating small object using such an instrument is called microscopy
The term microscopic means minute or very small, not visible with the eye unless aided by a microscope
From ancient times, man wanted to see things for smaller than could be perceived with the naked eye.
This led to the construction in the 16th century, of a magnifier composed of a single convex lens, and this in turn led to the eventual development of the microscope.
The most famous early pioneers in the history of microscope are Digges of England and Hans & Zcharias Janssen of Holland
It was Antony Van Leeuwenhoek who became the man to make and use a real microscope.
Leeuwenhoek microscope was called as single lens microscope because it had convex lens attached to metal holder and was focused using screws
This presentation include information about electron microscope & types of electron microscope i.e. SEM (Scanning electron microscope) & TEM (Transmission electron microscope).
An electron microscope is a microscope that uses a beam of scattered electrons as a source of illumination. It is used to get information about structure, topology, morphology & composition of materials. It has many advantages. Basically there are 4 types of electron microscope but here we will discuss only 2 types.
Transmission electron microscopy is a microscopy technique in which a beam of electrons is transmitted through an ultra-thin specimen, interacting with the specimen as it passes through it. Its resolution & magnification is about 10,000,000x. There are 5 types of transmission electron microscope i.e. BFTEM (Bright field transmision electron microscope), DFTEM (Dark field transmission electron microscope), HRTEM (High resolution transmission electron microscope), EFTEM (Energy filtered transmission electron microscope), ED (Electron diffraction). there are 4 techniques of TEM i.e. negative staining, shadow casting, Freeze fracture replication, freeze etching. It has many applications e.g, for the study of Cancer research, virology, chemical industry, electronic structure etc.
A scanning electron microscope is a type of electron microscope that produces images of a sample by scanning it with a focused beam of electrons. Types of signals produce by SEM include secondary electrons, back scattered electrons, X-rays, light rays. There are many advantages of SEM e.g, Btter resolution, fast imaging easy to operate, work with low voltage etc.
The microscope has evolved a lot from the time of Leeuwenhoek. This presentation gives a brief overview about the types of microscope their principle of function and application.
Introduction to microscopy
Different parts of a microscope & their function
Different types of microscopy
Different types of optical microscopy
Different types of electron microscopy
Different terms used in microscopy
Staining- Simple, Differential, Special
Gram Staining
Learn how to make invisible ink and then write with it that can't be seen with naked eye. Learn how to see this invisible writing when you. This is an amazing science experiment. Finally, learn how this works
Introduction
History
Compound microscope
Variants of microscopes
Dark field microscope
Phase contrast microscope
Fluorescent microscope
Polarising microscope
Electron microscope
A Microscope is an instrument for viewing objects that are too small to be seen by the naked/ unaided eyes.
In Greek micron= small
skopien=to look at
The science of investigating small object using such an instrument is called microscopy
The term microscopic means minute or very small, not visible with the eye unless aided by a microscope
From ancient times, man wanted to see things for smaller than could be perceived with the naked eye.
This led to the construction in the 16th century, of a magnifier composed of a single convex lens, and this in turn led to the eventual development of the microscope.
The most famous early pioneers in the history of microscope are Digges of England and Hans & Zcharias Janssen of Holland
It was Antony Van Leeuwenhoek who became the man to make and use a real microscope.
Leeuwenhoek microscope was called as single lens microscope because it had convex lens attached to metal holder and was focused using screws
This presentation include information about electron microscope & types of electron microscope i.e. SEM (Scanning electron microscope) & TEM (Transmission electron microscope).
An electron microscope is a microscope that uses a beam of scattered electrons as a source of illumination. It is used to get information about structure, topology, morphology & composition of materials. It has many advantages. Basically there are 4 types of electron microscope but here we will discuss only 2 types.
Transmission electron microscopy is a microscopy technique in which a beam of electrons is transmitted through an ultra-thin specimen, interacting with the specimen as it passes through it. Its resolution & magnification is about 10,000,000x. There are 5 types of transmission electron microscope i.e. BFTEM (Bright field transmision electron microscope), DFTEM (Dark field transmission electron microscope), HRTEM (High resolution transmission electron microscope), EFTEM (Energy filtered transmission electron microscope), ED (Electron diffraction). there are 4 techniques of TEM i.e. negative staining, shadow casting, Freeze fracture replication, freeze etching. It has many applications e.g, for the study of Cancer research, virology, chemical industry, electronic structure etc.
A scanning electron microscope is a type of electron microscope that produces images of a sample by scanning it with a focused beam of electrons. Types of signals produce by SEM include secondary electrons, back scattered electrons, X-rays, light rays. There are many advantages of SEM e.g, Btter resolution, fast imaging easy to operate, work with low voltage etc.
The microscope has evolved a lot from the time of Leeuwenhoek. This presentation gives a brief overview about the types of microscope their principle of function and application.
Introduction to microscopy
Different parts of a microscope & their function
Different types of microscopy
Different types of optical microscopy
Different types of electron microscopy
Different terms used in microscopy
Staining- Simple, Differential, Special
Gram Staining
Learn how to make invisible ink and then write with it that can't be seen with naked eye. Learn how to see this invisible writing when you. This is an amazing science experiment. Finally, learn how this works
Presentation to the Gulliver Forum in February 2007 on how my public library is using a blog and how other libraries are useing Web 2.0 tools to provide service to their users.
This presentation is about the introduction of microscopy, its history, parts of a microscope and different types of microscopes along with a brief discussion of their working principles.
Microscopy is the technique of using microscopes to observe and analyze objects that are too small to be seen by the naked eye. Microscopes are instruments that magnify and resolve the details of objects, allowing scientists and researchers to study the structure, composition, and behavior of materials and specimens at a microscopic level
Microscopy is the technique of using microscopes to observe and analyze objects that are too small to be seen by the naked eye. Microscopes are instruments that magnify and resolve the details of objects, allowing scientists and researchers to study the structure, composition, and behavior of materials and specimens at a microscopic level
microscope (1).pdf this is a project for for botany majorarpitakhairwar123
Name - Arpita khairwar
Class - B.sc 1st year
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Guided by - Dr. Pratima bisen
Submitted by - Arpita khairwar
While this ppt given by Dr pratima mam this ppt is a educational institution. My ppt is about microscope
Unit 8 - Information and Communication Technology (Paper I).pdfThiyagu K
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A review of the growth of the Israel Genealogy Research Association Database Collection for the last 12 months. Our collection is now passed the 3 million mark and still growing. See which archives have contributed the most. See the different types of records we have, and which years have had records added. You can also see what we have for the future.
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Acetabularia acetabulum is a single-celled green alga that in its vegetative state is morphologically differentiated into a basal rhizoid and an axially elongated stalk, which bears whorls of branching hairs. The single diploid nucleus resides in the rhizoid.
How to Build a Module in Odoo 17 Using the Scaffold MethodCeline George
Odoo provides an option for creating a module by using a single line command. By using this command the user can make a whole structure of a module. It is very easy for a beginner to make a module. There is no need to make each file manually. This slide will show how to create a module using the scaffold method.
2024.06.01 Introducing a competency framework for languag learning materials ...Sandy Millin
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Published classroom materials form the basis of syllabuses, drive teacher professional development, and have a potentially huge influence on learners, teachers and education systems. All teachers also create their own materials, whether a few sentences on a blackboard, a highly-structured fully-realised online course, or anything in between. Despite this, the knowledge and skills needed to create effective language learning materials are rarely part of teacher training, and are mostly learnt by trial and error.
Knowledge and skills frameworks, generally called competency frameworks, for ELT teachers, trainers and managers have existed for a few years now. However, until I created one for my MA dissertation, there wasn’t one drawing together what we need to know and do to be able to effectively produce language learning materials.
This webinar will introduce you to my framework, highlighting the key competencies I identified from my research. It will also show how anybody involved in language teaching (any language, not just English!), teacher training, managing schools or developing language learning materials can benefit from using the framework.
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Macroeconomics- Movie Location
This will be used as part of your Personal Professional Portfolio once graded.
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Prepare a presentation or a paper using research, basic comparative analysis, data organization and application of economic information. You will make an informed assessment of an economic climate outside of the United States to accomplish an entertainment industry objective.
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Exploiting Artificial Intelligence for Empowering Researchers and Faculty, In...Dr. Vinod Kumar Kanvaria
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4. History of the Micro(organism)scope
• 1590 –first compound
microscope
Discovery of
Microorganisms.
Anton van
Leeuwenhoek (16321723)
– first person to
observe and describe
micro-organisms
accurately
The way how he found micro-organism’s….
6. Parts of Microscope,,,
• Eyepiece Lens: the lens at the top that you look through. They are usually
10X or 15X power.
• Tube: Connects the eyepiece to the objective lenses.
• Arm: Supports the tube and connects it to the base
• Base: The bottom of the microscope, used for support
• Illuminator: A steady light source (110 volts) used in place of a mirror. If
your microscope has a mirror, it is used to reflect light from an external
light source up through the bottom of the stage.
7. Cont,,,
• Stage: The flat platform where you place your slides. Stage clips hold the
slides in place. If your microscope has a mechanical stage, you will be able
to move the slide around by turning two knobs. One moves it left and
right, the other moves it up and down.
• Revolving Nosepiece or Turret: This is the part that holds two or more
objective lenses and can be rotated to easily change power.
• Rack Stop: This is an adjustment that determines how close the objective
lens can get to the slide.
• Diaphragm or Iris: This diaphragm has different sized holes and is used
to vary the intensity and size of the cone of light that is projected upward
into the slide.
8. Lens
Objective lens
Condenser Lens
Usually you will find 3 or 4 objective lenses on
a microscope
The purpose of the condenser lens is to focus
the light onto the specimen
It consist of 4X, 10X, 40X and 100X powers.
Condenser lenses are most useful at the highest
powers (400X and above).
When coupled with a 10X (most common)
eyepiece lens, we get total magnifications of
40X (4X times 10X), 100X , 400X and 1000X
Microscopes with in stage condenser lenses
render a sharper image than those with no lens
(at 400X)
If the microscope has a maximum power of
400X, you will get the maximum benefit by
using a condenser lenses rated at 0.65 NA or
greater
9. Microscope Vocabulary
• Magnification: increase of an object’s
apparent size
• Resolution: power to show details clearly
Both are needed to see a clear image
10. Types of Microscopy
•
•
•
•
•
•
•
TEM
Compound Light Microscope
Phase contrast microscope
Dissection or stereoscope
Electron Microscope
Transmission Electron Microscope
Scanning Electron Microscope
Flourescence microscope
SEM
11. Light mechanism
• What is Light???
Visible light (commonly referred to simply as light) is an
electromagnetic radiation that is visible to the human eye, and
is responsible for the sense of sight.
Visible light has a wavelength in the range of about
380 nanometres (nm), or 380 10−9 m, to about
740 nanometres.
The natural agent that stimulates sight and makes things visible.
Used for illuminations…..
12. Angle of Incidence
• Angle of incidence is a measure of deviation
of something from "straight on―
13. Snell’s Law
Snel l 's l aw(al so know as t he Snel l –D
n
escar t es l awand t he l awof
r ef r act i on) i s a f or m a used t o descr i be t he r el at i onshi p
ul
bet w
een t he angl es of i nci dence and r ef r act i on, w
hen r ef er r i ng t o
l i ght or ot her w
aves passi ng t hr ough a boundar y bet w
een t w
o
di f f er ent i sot r opi c m a, such as w er , gl ass and ai r .
edi
at
14. Cont,,,
Refraction of light at the interface between two
media of different refractive indices, with n2 >
n1. Since the velocity is lower in the second
medium (v2 < v1), the angle of refraction θ2 is
less than the angle of incidence θ1; that is, the
ray in the higher-index medium is closer to the
normal.
15. Do light waves have amplitude???
•
Of course it has,
•
Yes… Waves in general have three properties
Frequency (related to wavelength), Amplitude, and Speed
Freq- No of cycles.
•
•
•
Frequency tells us how many waves are passing a point per second, the inverse
of time.
Wavelength tells us the length of those waves in metres, almost like
adisplacement.
If we multiply these two together, we are really multiplying 1/s and m… which
gives us m/s, the velocity of the wave!
17. Lenses and the Bending of Light
• Light is refracted (bent) when passing from
one medium to another
• Refractive index
– a measure of how greatly a substance slows the
velocity of light ,
where c is the speed of light in vacuum and v is the speed of light in the substance
• Direction and magnitude of bending is
Determined by the refractive indexes of the
two media forming the interface
.
17
18. Focal point and Focal length
• Focus light rays at a specific
place called the focal point
• Distance between center of lens and
focal point is the focal length
• Strength of lens related to focal
length
•
short focal length
magnification
more
18
19. The Light Microscope
• Many types
–
–
–
–
bright-field microscope
dark-field microscope
phase-contrast microscope
fluorescence microscopes
• are compound microscopes
– image formed by action of 2 lenses
19
21. The Bright-Field Microscope
• Produces a dark image against a brighter
background
• Has several objective lenses
– parfocal microscopes remain in focus when
objectives are changed
• Total magnification
– product of the magnifications of the ocular lens
and the objective lens
21
22. Microscope Resolution
• Ability of a lens to separate or distinguish small objects that are close
together
• Wavelength of light used is major factor in resolution
shorter wavelength greater resolution
22
24. The Dark-Field Microscope
• Produces a bright image of the object against a dark
background
• Used to observe living, unstained preparations
24
25. The Phase-Contrast Microscope
• Enhances the contrast between intracellular structures
having slight differences in refractive index
• Excellent way to observe living cells
Working Principle,,,
25
27. The Differential Interference
Contrast Microscope
• Creates image by detecting differences in
refractive indices and thickness of different
parts of specimen
• Excellent way to observe living cells
27