Classification of species, The definitive, intermediate host, mode of infection, life cycle of malaria. Plasmodium falciparum, cerebral malaria, the pathogenesis of malaria, clinical features, algid malaria, black water fever, Lab diagnosis- microscopic, QBC, Thick and thin smears, Fluorescent microscopy.
Non-microscopic - Molecular methods PCR, Antigen dectection
Treatment- NVBDCP, prevention
Malaria is a Vector-borne parasitic disease found in 91 countries worldwide. >120 Plasmodium species infect mammals, birds, and reptiles. Only five are known to infect human. Plasmodium falciparum causes majority of deaths due to high levels of parasitemia, sequestration of parasite in critical organs and causing severe anemia
Ascariasis is an infection of the small intestine caused by Ascaris lumbricoides, a species of roundworm. Infections have no symptoms in more than 85% of cases, especially if the number of worms is small. Symptomatic ascariasis may manifest as growth retardation, pneumonitis, intestinal obstruction, or hepatobiliary and pancreatic injury.
Malaria is a Vector-borne parasitic disease found in 91 countries worldwide. >120 Plasmodium species infect mammals, birds, and reptiles. Only five are known to infect human. Plasmodium falciparum causes majority of deaths due to high levels of parasitemia, sequestration of parasite in critical organs and causing severe anemia
Ascariasis is an infection of the small intestine caused by Ascaris lumbricoides, a species of roundworm. Infections have no symptoms in more than 85% of cases, especially if the number of worms is small. Symptomatic ascariasis may manifest as growth retardation, pneumonitis, intestinal obstruction, or hepatobiliary and pancreatic injury.
coccidian parasite is a very important topic for pg entrance........so every important point about it have been discussed in detail......take a look at it...
coccidian parasite is a very important topic for pg entrance........so every important point about it have been discussed in detail......take a look at it...
Malaria is a life-threatening disease caused by parasites that are transmitted to people through the bites of infected female Anopheles mosquitoes. It is one of the world’s leading causes of death, particularly among children in developing countries.
Introduction Malaria
Chronology in Malaria
Epidemology
Life cycle
Pathogenesis and clinical feature
Lab Test
Treatment of Malaria
Prevention of Malaria
able of ContentsIntroductionObjectives of Giemsa stainPrincipleReagents UsedProcedureStaining procedure 1: Thin Film stainingStaining Procedure 2: Thick Film StainingResultsInterpretation/ConclusionApplications Giemsa stainAdvantagesLimitationsReferencesFour Charged in Plot to Kidnap an Iranian Journalist in New YorkIntroductionGiemsa stain was a name adopted from a Germany Chemist scientist, for his application of a combination of reagents in demonstrating the presence of parasites in malaria.It belongs to a group of stains known as Romanowsky stains. These are neutral stains made up of a mixture of oxidized methylene blue, azure, and Eosin Y and they performed on an air-dried slide that is post-fixed with methanol. Romanowsky stains are applied in the differentiation of cells, pathological examinations of samples like blood and bone marrow films and demonstration of parasites e.g malaria. There are four types of Romanoswsky stains:Giemsa stainJenner StainWright stainMay-Grunwald StainLeishman stainObjectives of Giemsa stainTo accurately prepare the Giemsa stain stock solutionTo stain and identify blood cellsTo differentiate blood cells nuclei from the cytoplasmPrincipleGiemsa stain is a gold standard staining technique that is used for both thin and thick smears to examine blood for malaria parasites, a routine check-up for other blood parasites and to morphologically differentiate the nuclear and cytoplasm of Erythrocytes, leucocytes and Platelets and parasites.Like any type of Romanowsky stains, it composed of both the Acidic and Basic dyes, in relation to affinities of acidity and basicity for blood cells. Azure and methylene blue, a basic dye binds to the acid nucleus producing blue-purple color. Eosin is an acidic dye that is attracted to the cytoplasm and cytoplasmic granules which are alkaline-producing red coloration. The stain must be buffered with water to pH 6.8 or 7.2, to precipitate the dyes to bind simple materials.Classically, Giemsa stain is a differential stain which is made up of a combination of reagents (Azure, Methylene blue, and Eosin dye) used widely in cytogenetics and histopathology for the diagnosis of:Malaria, spirochetes and other blood parasitesChlamydia trachomatis inclusion bodiesBorrelia sppYersinia pestisHistoplasma sppPneumocystis jiroveci cystsReagents UsedMethanolGiemsa powderGlycerinWater (Buffer)ProcedurePreparation of the Giemsa Stain Stock solution (500ml)Into 250ml of methanol, add 3.8g of Giemsa powder and dissolve.Heat the solution up to ~60oCThen, add 250ml of glycerin to the solution, slowly.Filter the solution and leave it to stand for about 1-2 months before use.Preparation of Working solutionAdd 10ml of stock solution to 80ml of distilled water and 10ml of methanolStaining procedure 1: Thin Film stainingOn a clean dry microscopic glass slide, make a thin film of the specimen (blood) and leave to air dry.dip the smear (2-3 dips) into pure methanol for fixation of the
Protozoan parasites characterized by the production of spore-like oocysts containing sporozoites were known as sporozoa.
They live intracellularly, at least during part of their life cycle
Vasculitis
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Define and classify vasculitis.
Describe the cause, pathogenesis, morphology, and clinical presentation of various types of vasculitis.
Pharmacology- anti-fungal drugs.Classification and details on polyenes, echinocandins, griseofulvin, azoles, terbinafine, and topical azoles.
Their uses, side effects, adverse effects are mentioned with the mechanism of action.
Malignant bone tumors- clinical presentation, epidemiology, pathological findings, radiological findings, cases
Includes osteosarcoma, Ewing's sarcoma, and chondrosarcoma in detail.
biochemistry- Role of insulin in metabolism- PHYSIOLOGICAL ACTION OF INSULINSaachiGupta4
INSULIN- ANTILIPOLYTIC, LIPOGENESIS, ANTI- KETOGENIC EFFECT, UTILIZATION OF GLUCOSE BY TISSUES, HYPOGLYCEMIC EFFECT
PHYSIOLOGICAL FUNCTION OF INSULIN
BIOCHEMISTRY
Kidney development - embryology of urinary systemSaachiGupta4
Part of the development of the urinary system
The ascent of the kidney, stages of development of kidney
discussion about pronephros, mesonephros, metanephros.
Development of liver, pancreas, spleen and extrahepatic biliary apparatusSaachiGupta4
Embryology- anatomy
Topic: Development of liver, pancreas, spleen, and extrahepatic biliary apparatus.
For M.B.B.S. students. It gives knowledge on the development of the organs mentioned above and their developmental anomalies
Development of liver , extrahepatic biliary apparatus , pancreas and spleen.SaachiGupta4
embryology- development and developmental anomalies of the liver, extrahepatic biliary apparatus, pancreas and spleen.
Stages of development of liver, reidel's lobe, annular pancreas.
Alimentary tract embryology
URINE FORMATION- 3 processes
GFR, Tubular reabsorption and tubular secretion.
FILTRATION MEMBRANE
GFR regulation, Tubular reabsorption regulation and transport explanation
Palestine last event orientationfvgnh .pptxRaedMohamed3
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The Indian economy is classified into different sectors to simplify the analysis and understanding of economic activities. For Class 10, it's essential to grasp the sectors of the Indian economy, understand their characteristics, and recognize their importance. This guide will provide detailed notes on the Sectors of the Indian Economy Class 10, using specific long-tail keywords to enhance comprehension.
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Model Attribute Check Company Auto PropertyCeline George
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Synthetic Fiber Construction in lab .pptxPavel ( NSTU)
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Welcome to TechSoup New Member Orientation and Q&A (May 2024).pdfTechSoup
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22. The Human Cycle: Pre-erythrocytic Schizogony
In 8 days
Merozoites liberated per schizont
are 10,000 in number.
Some sporozoites go into dormant state, known as Hypnozoites,(clinical relapses)
23. (infect reticulocytes and young RBCs)
Erythrocytic Schizogony (48 hrs)
All stages of Erythrocytic Schizogony can be seen
in the peripheral blood smear.
24. Erythrocytic Stages of Plasmodium Vivax
(12-24 merozoites
per Schizont)
Trophozoite
Malaria pigment
Female
Male
Schuffner's dots
26. The Human Cycle: Pre-erythrocytic Schizogony
In 6 days
Merozoites liberated per schizont
are 30,000 in number.
No hypnozoites
27. (infect both young and mature RBCs)
(Late trophozoites, schizonts are formed in
capillaries of internal organs, and not in
peripheral blood)
Gametogony after 10 days
Female gametocyte
Male gametocyte
Multiple rings,
accole form
in normal sized RBCs
28. Erythrocytic Stages of Plasmodium falciparum
Trophozoite
Malaria pigment
Maurer's dots
Male
Female
Gametocytes
schizonts
multiple rings
accole form
29. Erythrocytic Stages of Plasmodium falciparum
Peripheral circulation
Vascular beds of internal organs
Trophozoite
Gametocytes
schizonts
30.
31. Pathogenesis
The clinical manifestations in malaria are due to :
• Response of host to parasitic antigens
• Anaemia in malaria is due to increased clearance
of both parasitized and non parasitized RBCs by
the spleen.
• Tissue hypoxia due to obstruction of blood flow
38. Cerebral Malaria
üP.falciparum in capillaries of internal organs, secrete protein
knobs on surface of RBCs.
üThis promotes aggregation of infected RBCs to non infected
RBCs and capillary endothelial cells, causing capillary
plugging in brain.
üIt leads to anoxia, ischaemia and haemorrhage in brain.
üManifested by headache, hyperpyrexia, coma, paralysis.
Capillary plugging
39. Algid Malaria
•Patient presents with severe abdominal
pain, vomiting, diarrhoea and profound
shock.
•This syndrome is characterized by
peripheral circulatory failure, rapid
thready pulse, low BP, cold clammy skin.
40. Septicemic Malaria
It is characterized by high
continuous fever with dissemination
of the parasite to various organs,
leading to multi organ failure.
Death occurs in 80% of the cases.
41. Blackwater Fever
•Seen in falciparum malaria patients on
inadequate treatment with quinine.
• Development of anti-erythrocyte antibodies.
•Massive, sudden, intravascular hemolysis
occurs.
•characterized by massive absorption of
hemoglobin by the renal tubules, leading to
haemoglobinuria, excessive pigment in urine
gives brown black colour to urine.
44. Peripheral blood smear examination
• For Microscopy
ü thick and thin blood film.
• Romanowsky stain
i. Field’s stain
ii. Giemsa stain
iii. Leishman stain
iv. Jaswant Singh and Bhattacharya (J.S.B) stain
45.
46. • THICK FILM
§ Lysed RBCs, many layers
§ Larger volume
(0.25 µl blood/100 fields)
§ Good screening test
• THIN FILM
§ Single layer of RBCs
§ Small volume
(0.005µl blood/100 fields)
§ Species differentiation
47.
48.
49.
50.
51. Rapid immunochromatography Test for
Antigen detection
Histidine- rich
protein II (HRP – II)
: P. falciparum.
Parasite lactate
dehydrogenase
(pLDH)
Parasite aldolase:
all Plasmodium
spp.
52.
53.
54.
55. Culture
RPMI 1640 medium:-
(Roswell Park
Memorial Institute
and 1640 denotes the
number of passages).
Dulbecco’s Modified
Eagle
Medium(DMEM)
58. Treatment of vivax malaria
(NVBDCP guideline) • Chloroquine :
25mg/kg divided
over three days,
i.e.10 mg/kg on
day 1and 2 and 5
mg/kg on day 3
• Primaquine: 0.25
mg/kg daily for
14 days
59. Treatment of falciparum
malaria (NVBDCP
guideline,India)
• ACT-SP (artesunate-
sulfadoxine/pyrimethami
ne)
• Artesunate (25mg/kg)
for 3 days
• Sulfadoxine (25mg/kg)/
pyrimethamine(1.25mg/
kg), 1 tablet given on
first day
• Primaquine
60. Prevention
• For adult:
• Residual spraying:
dichlorodiphenyltrichloroethane (DDT) and
malathion.
• Individual protection: bed nets and protective
clothing
61. For larva
• Larvicide- mineral oil or Paris green
• Biological larvicide- Gumbusia affinis
(fish) and Bacillus thuringensis (bacteria)
• Source reduction ( to reduce the
mosquito breeding sites) includes
environmental sanitation, water
management and improvement of the
drainage system.