The document discusses the principles and components of gas chromatography-mass spectrometry (GC-MS). GC-MS combines gas chromatography and mass spectrometry to identify unknown chemical compounds in a sample. In GC-MS, the sample is vaporized and injected into a GC where components are separated by volatility. The separated components enter the mass spectrometer where they are ionized and fragmented, producing mass spectra that can identify each component. The combined GC-MS technique provides both separation and identification of compounds in a single analysis.
1. It is one of the type of Hyphenated technique.
2. It is a combination of gas chromatographic technique and spectroscopic technique.
3. It is having a high resolution capacity.
4. It is used has volatile and Non-volatile compounds.
5. It is used for qualitative and quantitative analysis.
GCMS & LCMS
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Sub :- Advanced Analytical Techniques
M.Pharmacy Sem1
Savitribai Phule Pune University
Contents :-
GC-MS
Introduction
Principle
Instrumentation
Application
LC-MS
Introduction
Principle
Instrumentation
Application
Introduction to Gas chromatography-Mass spectroscopy
Gas chromatography-Mass spectroscopy is one of the so-called hyphenated analytical techniques. It is actually two techniques that are combined to form a single method of analyzing mixtures of chemicals
GC-MS is an instrumental technique, comprising a gas chromatograph coupled to a mass spectrometer by which complex mixtures of chemicals may be separated, identified & quantified. In order to a compound to be analysed by GC-MS it must be sufficiently volatile & thermally stable.
Principle :-
The Sample solution is injected into the GC inlet where it is vapourized & swept onto a chromatographic column by the carrier gas ( usually helium). The sample flows through the column & compounds comprising the mixture of interest are separated by virtue of their relative interaction with the coating of the column (stationery phase) & the carrier gas (mobile phase). The later part of the column passes through a heated transfer line & ends at the entrance to ion source where compounds eluting from the column are converted to ions
1. It is one of the type of Hyphenated technique.
2. It is a combination of gas chromatographic technique and spectroscopic technique.
3. It is having a high resolution capacity.
4. It is used has volatile and Non-volatile compounds.
5. It is used for qualitative and quantitative analysis.
GCMS & LCMS
htps://youtube.com/vishalshelke99
https://instagram.com/vishal_stagram
Sub :- Advanced Analytical Techniques
M.Pharmacy Sem1
Savitribai Phule Pune University
Contents :-
GC-MS
Introduction
Principle
Instrumentation
Application
LC-MS
Introduction
Principle
Instrumentation
Application
Introduction to Gas chromatography-Mass spectroscopy
Gas chromatography-Mass spectroscopy is one of the so-called hyphenated analytical techniques. It is actually two techniques that are combined to form a single method of analyzing mixtures of chemicals
GC-MS is an instrumental technique, comprising a gas chromatograph coupled to a mass spectrometer by which complex mixtures of chemicals may be separated, identified & quantified. In order to a compound to be analysed by GC-MS it must be sufficiently volatile & thermally stable.
Principle :-
The Sample solution is injected into the GC inlet where it is vapourized & swept onto a chromatographic column by the carrier gas ( usually helium). The sample flows through the column & compounds comprising the mixture of interest are separated by virtue of their relative interaction with the coating of the column (stationery phase) & the carrier gas (mobile phase). The later part of the column passes through a heated transfer line & ends at the entrance to ion source where compounds eluting from the column are converted to ions
GAS CHROMATOGRAPHY-MASS SPECTROSCOPY [GC-MS]Shikha Popali
THIS PRESENTATION GIVES A DETAIL ACCOUNT ON THE GC-MS WITH ITS INTRODUCTION, BASIC PRINCIPLE OF BOTH COMBINED AND INDIVIDUALLY WITH ITS INSTRUMENTATION, APPLICATION AND EXAMPLES, MAKES EASY TO COLLECT ALL THE DATA AT A PLACE ACCORDING TO THE M.PHARM SYLLABUS S PER PCI
Gas chromatography-Mass spectrometry (GC-MS)Saira Fatima
PRESENTED BY
SAIRA FATIMA
SABAHAT MEHMOOD
SANA USMAN
MSc 4 (2018-2020)
Department of MicroBiology & Molecular Genetics
University of the Punjab
Lahore, Pakistan
This ppt consist of basic principle of GC-MS, instrumentation of GC-MS, components of GC-MS ,Advantages and disadvantages of GC-MS and application of GC-MS
fluid chromatography (SFC) can be used on an analytical
scale.
It is a combination of High performance liquid chromatography (HPLC)
and Gas chromatography (GC).
It can be used with non-volatile and thermally labile analytes.
It can be used with the universal flame ionization detector.
It is important to producing narrower peaks due to rapid diffusion.
It is important for the chiral separations and analysis of high-molecularweight
hydrocarbons.
Supercritical fluids are suitable as a substitute for organic solvents in a
range of industrial and laboratory processes.
Atomic absorption spectroscopy, History, atomization techniques, and instrume...Muhammad Asif Shaheeen
History, principle, types, instrumentation, comparison with atomic emission spectroscopy, interference, advantages and disadvantages of different types of atomization techniques.
GAS CHROMATOGRAPHY-MASS SPECTROSCOPY [GC-MS]Shikha Popali
THIS PRESENTATION GIVES A DETAIL ACCOUNT ON THE GC-MS WITH ITS INTRODUCTION, BASIC PRINCIPLE OF BOTH COMBINED AND INDIVIDUALLY WITH ITS INSTRUMENTATION, APPLICATION AND EXAMPLES, MAKES EASY TO COLLECT ALL THE DATA AT A PLACE ACCORDING TO THE M.PHARM SYLLABUS S PER PCI
Gas chromatography-Mass spectrometry (GC-MS)Saira Fatima
PRESENTED BY
SAIRA FATIMA
SABAHAT MEHMOOD
SANA USMAN
MSc 4 (2018-2020)
Department of MicroBiology & Molecular Genetics
University of the Punjab
Lahore, Pakistan
This ppt consist of basic principle of GC-MS, instrumentation of GC-MS, components of GC-MS ,Advantages and disadvantages of GC-MS and application of GC-MS
fluid chromatography (SFC) can be used on an analytical
scale.
It is a combination of High performance liquid chromatography (HPLC)
and Gas chromatography (GC).
It can be used with non-volatile and thermally labile analytes.
It can be used with the universal flame ionization detector.
It is important to producing narrower peaks due to rapid diffusion.
It is important for the chiral separations and analysis of high-molecularweight
hydrocarbons.
Supercritical fluids are suitable as a substitute for organic solvents in a
range of industrial and laboratory processes.
Atomic absorption spectroscopy, History, atomization techniques, and instrume...Muhammad Asif Shaheeen
History, principle, types, instrumentation, comparison with atomic emission spectroscopy, interference, advantages and disadvantages of different types of atomization techniques.
The Viking labelled release experiment: life on Mars?Neil Saunders
This is a very old talk from around 1999 that I gave to my department at the Free University of Amsterdam. It\'s very out of date now, but still interesting.
Biodiversity, Microbial Biodiversity, Bacterial Biodiveristy, Archae Biodiversity, Protozoa Biodiversity, Fungal Biodiversity, Origin of Life, Origin of Life on Earth, Chemical Evolution, Physical Evolution, Biological Evolution
An Analysis of Amoxicillin Through GCMS and Later FTIRKirk Teegardin
The objective of this experiment was to analyze different brands of Amoxicillin and determine if they contain the stated amount of Amoxicillin as labeled. This was to be accomplished through the use of Gas Chromatography/Mass Spectrum, but resulted in having to be completed through Infrared Spectroscopy due to the complexity of Amoxicillin.
This presentation was presented when I was MVSc 1st year student, hence this ppt will provide a piece of basic information about the use of Chromatography.in toxicology. Principle of its work and related terminology are explained in this ppt.
Introduction to gas Chromatography
,Principle of gas chromatography
Instrumentation of gas Chromatography
Type of detectors of gas chromatography
Advantages of gas chromatography
Disadvantages of gas chromatography
Applications of gas chromatography
Hyphenated techniques have received ever-increasing attention as the principal means to solve complex analytical problems.
Hyphenated techniques are widely used in chemistry and biochemistry and used for both quantitative and qualitative analysis of unknown compounds in complex natural product extracts or fraction and estimation of protein samples also.
The hyphenated technique is a combination or coupling of two analytical techniques with the help of a proper interface.
The aim of the coupling is to obtain an information-rich detection for both identification and quantification compared to that with a single analytical technique.
002. Cephalosporins for students 2023 Prof. P. Ravisankar.pdfDr. Ravi Sankar
Cephalosporins, Why Cephalosporins? Advantages of cephalosporins over penicillin, Mechanism of action of cephalosporins, Classification of cephalosporins, Structures of some important cephalosporins and cephamycins, Oximinocephalosporins, SAR of cephalosporins,Hydrolytic reactions, degradation and stability of cephalosporins, Uses of cephalosporins, Comparison between 6-APA and 7-ACA and penam and cepham.
1a. Prof. P. Ravisankar, Vignan Pharmacy College, Vadlamudi, Guntur, AP., Ind...Dr. Ravi Sankar
Antibiotics, characteristics of an antibiotic, Brief historical background of antibiotics, Modern History of antibiotics, Classification of antibiotics, four groups of beta-lactam antibiotics, Classification of Penicillins, Penicillinase resistant Penicillins, Narrow spectrum anti-staphylococcal Penicillins, Amino Penicillins, Nomenclature of Penicillins, Stereochemistry of Penicillins, Hydrolysis of Penicillins, SAR of Penicillins, Mechanism of Penicillins, Beta-lactamase inhibitors, Therapeutic uses of Penicillins, Penicillin advantages, and disadvantages, Hypersensitivity or allergic reactions of Penicillins.
Tetracyclines are Octahydro napthacene derivatives which are bacteriostatic potent broad spectrum antibiotics and are the most widely prescribed form of antibiotic after penicillins.
TETRA means = four
CYCL means = hydrocarbon rings
INE means = derivation.
Tetracyclines are introduced 50 years ago as potent broad spectrum antibiotics.
They are biosynthesized form acetic acid and propionic acid units in microorganisms.
Finally, one of the best ways of reducing cancer is …..
Cancer prevention is an essential component of all cancer control plans because about 40 % of cancers are preventable.
Firstly…Public education campaigns are important in highlighting the dangers of smoking because possibly as many as 22 % (in the UK 30%) of cancers are caused by smoking.
Secondly, another 30% of cancers are diet-related. Decades of research have clearly shown that by living a healthy life, people can reduce the risk of developing the disease.
Screening tests help in finding Cancer earlier before developing any symptoms.
The benefits of eating high-fiber foods, fruit, and vegetables.
Epigallocatechin gallate, an antioxidant present in green tea, is another potential protective agent.
Finally, The new research funded by the UK and USA which aims to show the number of cancer cases could be prevented by known lifestyle and environmental factors,
like being a non-smoker, keeping a healthy weight, drinking less alcohol, eating a healthy balanced diet, and avoiding being exposed to certain infections or radiation.
Every country, regardless of resource level, can take steps to curb the cancer epidemic, save lives and prevent unnecessary suffering.
Finally, cancer prevention efforts should be preceded by a systematic planning process on cancer control.
Antibiotics definition, Early and modern history, classification of antibiotics, Mechanism of antibacterial action, bacterial cell and drug targets, penicillins nomenclature, degradation reactions of penicillins, medical classification of penicillins, SAR of penicillins, Mechanism of penicillins, Betalactamase inhibitors, Therapeutic uses of penicillins, toxicity of penicillins, Side effects of penicillins.
NOVEL SEPARATION AND QUANTITATIVE DETERMINATION OF LEVOFLOXACIN, PRULIFLOXACI...Dr. Ravi Sankar
The core AIM of the present study is to develop a novel, rapid, precise and accurate RP-HPLC method for simultaneous separation and quantification of six fluoroquinolones OF LEVOFLOXACIN (LEVO), PRULIFLOXACIN (PRFX), GATIFLOXACIN (GATI), SPARFLOXACIN (SPAR), MOXIFLOXAXIN (MOXI) AND BALOFLOXACIN (BALO) for the the day to day analysis.
The author felt that a novel single method for separation and quantification of all the above said drugs on single chromatographic system without any minor changes in detection wavelength and mobile phase composition.
To develop rapid, sensitive and economical analytical method based on HPLC for separation and estimation of six fluoroquinolones pharmaceutical dosage forms.
To develop method with shorter run time and better sensitivity.
Reducing the solvent consumption to make it more eco-friendly.
Avoid the column damage by minimizing the buffer strength and pH of mobile phase than reported methods.
To validate the method for different parameters like Accuracy, Precision, Linearity, specificity, Robustness International Conference on Harmonization ICH Q2(R1) guidelines..
To apply the developed RP-HPLC method in the analysis of pharmaceutical formulations.
Analytical method development and validation of novel Levofloxacin, Pruliflox...Dr. Ravi Sankar
For the first time simple, selective, sensitive RP-HPLC method was developed for the separation and quantitative development of LEVO, PRFX, GATI, SPAR, MOXI and BALO relating to fluoroquinolone anti bacterials in pharmaceutical dosage forms. The most important advantage of developed method was that the 6 separate drugs can be determined on a single chromatographic system without modifications in detection wavelength and mobile phase by RP-HPLC. The chromatographic separation of the selected drugs was carried out on Welchrom C18 column consisting of 250 mm X 4.6 mm, 5 µm particle size utilizing mixture of 10 mM phosphate buffer (pH 3.1): Acetonitrile in the ratio of 70:30,v/v as mobile phase at the flow rate of 1mL/min with detection wave length at 293 nm by using UV spectrophotometric detector with total run time of 10 minutes and 3.613, 4.230, 4.707, 5.497, 5.880 and 6.253 minutes of retention time obtained for LEVO, PRFX, GATI, SPAR, MOXI and BALO respectively. All calibration curves for six drugs showed indicated linearity over a concentration range of 2-10 µg/mL. The results regarding to limit of detection (LOD) and limit of quantitation (LOQ) for LEVO, PRFX, GATI, SPAR, MOXI and BALO were found to be 0.116 µg/mL and 0.348 µg/mL; 0.152 µg/mL and 0.460 µg/mL; 0.084 µg/mL and 0.255 µg/mL; 0.186 µg/mL and 0.558 µg/mL, 0.162 and 0.493, 0.112 and 0.390 respectively. These results clearly show low values of LOD and LOQ. The said developed method was ultimately utilized for quantification of marketed formulation.
NOVEL SIMULTANEOUS SEPARATION AND QUANTITATIVE DETERMINATION OF FOUR SARTANS...Dr. Ravi Sankar
The core AIM of the present study is to develop a novel, rapid, precise and accurate RP-HPLC method for simultaneous separation and quantification of Hydrochlorothiazide along with four sartans Telmisartan(TELM), Losartan(LOSA), Olmesartan(OLME) and Valsartan(VALS).
To develop Novel methods for separation and quantification of all the above said drugs on single chromatographic system without any minor changes in detection wavelength and mobile phase composition.
Total quality management (TQM), and current Good Manufacturing Practice (cGMP...Dr. Ravi Sankar
TQM, cGMP, Introduction, Definition, Importance, TQM frame work, Key concepts (Principles) of TQM, specific steps in the cycle, Benefits of TQM, cGMP, principles of GMP, Improtance of GMP, why GMP established?, difference between GMP and cGMP, GMP and cGMP regulations, code of federal regulations.
Tetracyclines BY Dr. P. Ravisankar M. Pharm., Ph.D.Dr. Ravi Sankar
Tetracyclines by Dr. P. Ravisankar M. Pharm., Ph.D.
Definition
Introduction
Classification
Historical background
Sources
Chemistry
SAR of tetracyclines
Mechanism of action of tetracyclines
Spectrum of activity
Uses of tetracyclines
Side effects of tetracyclines
Dr. P. Ravisankar M. Pharm., Ph.D.
Vitamin A
Vitamin D
Vitamin E
Vitamin K
Definition
Introduction
Classification
Structures,Functions,Deficiency,Diseases,Toxicity and uses.
Introduction to diuretics.
Therapeutic approaches.
Normal physiology of urine formation.
Classification of drugs .
Mechanism of action of Acetazolamide.
Mechanism of action of Thiazides.
Mechanism of action of Loop diuretics.
Mechanism of action of potassium sparing diuretics &aldosterone antagonists.
Students, digital devices and success - Andreas Schleicher - 27 May 2024..pptxEduSkills OECD
Andreas Schleicher presents at the OECD webinar ‘Digital devices in schools: detrimental distraction or secret to success?’ on 27 May 2024. The presentation was based on findings from PISA 2022 results and the webinar helped launch the PISA in Focus ‘Managing screen time: How to protect and equip students against distraction’ https://www.oecd-ilibrary.org/education/managing-screen-time_7c225af4-en and the OECD Education Policy Perspective ‘Students, digital devices and success’ can be found here - https://oe.cd/il/5yV
Operation “Blue Star” is the only event in the history of Independent India where the state went into war with its own people. Even after about 40 years it is not clear if it was culmination of states anger over people of the region, a political game of power or start of dictatorial chapter in the democratic setup.
The people of Punjab felt alienated from main stream due to denial of their just demands during a long democratic struggle since independence. As it happen all over the word, it led to militant struggle with great loss of lives of military, police and civilian personnel. Killing of Indira Gandhi and massacre of innocent Sikhs in Delhi and other India cities was also associated with this movement.
How to Split Bills in the Odoo 17 POS ModuleCeline George
Bills have a main role in point of sale procedure. It will help to track sales, handling payments and giving receipts to customers. Bill splitting also has an important role in POS. For example, If some friends come together for dinner and if they want to divide the bill then it is possible by POS bill splitting. This slide will show how to split bills in odoo 17 POS.
How to Make a Field invisible in Odoo 17Celine George
It is possible to hide or invisible some fields in odoo. Commonly using “invisible” attribute in the field definition to invisible the fields. This slide will show how to make a field invisible in odoo 17.
Synthetic Fiber Construction in lab .pptxPavel ( NSTU)
Synthetic fiber production is a fascinating and complex field that blends chemistry, engineering, and environmental science. By understanding these aspects, students can gain a comprehensive view of synthetic fiber production, its impact on society and the environment, and the potential for future innovations. Synthetic fibers play a crucial role in modern society, impacting various aspects of daily life, industry, and the environment. ynthetic fibers are integral to modern life, offering a range of benefits from cost-effectiveness and versatility to innovative applications and performance characteristics. While they pose environmental challenges, ongoing research and development aim to create more sustainable and eco-friendly alternatives. Understanding the importance of synthetic fibers helps in appreciating their role in the economy, industry, and daily life, while also emphasizing the need for sustainable practices and innovation.
How to Create Map Views in the Odoo 17 ERPCeline George
The map views are useful for providing a geographical representation of data. They allow users to visualize and analyze the data in a more intuitive manner.
Palestine last event orientationfvgnh .pptxRaedMohamed3
An EFL lesson about the current events in Palestine. It is intended to be for intermediate students who wish to increase their listening skills through a short lesson in power point.
2024.06.01 Introducing a competency framework for languag learning materials ...Sandy Millin
http://sandymillin.wordpress.com/iateflwebinar2024
Published classroom materials form the basis of syllabuses, drive teacher professional development, and have a potentially huge influence on learners, teachers and education systems. All teachers also create their own materials, whether a few sentences on a blackboard, a highly-structured fully-realised online course, or anything in between. Despite this, the knowledge and skills needed to create effective language learning materials are rarely part of teacher training, and are mostly learnt by trial and error.
Knowledge and skills frameworks, generally called competency frameworks, for ELT teachers, trainers and managers have existed for a few years now. However, until I created one for my MA dissertation, there wasn’t one drawing together what we need to know and do to be able to effectively produce language learning materials.
This webinar will introduce you to my framework, highlighting the key competencies I identified from my research. It will also show how anybody involved in language teaching (any language, not just English!), teacher training, managing schools or developing language learning materials can benefit from using the framework.
The Art Pastor's Guide to Sabbath | Steve ThomasonSteve Thomason
What is the purpose of the Sabbath Law in the Torah. It is interesting to compare how the context of the law shifts from Exodus to Deuteronomy. Who gets to rest, and why?
This is a presentation by Dada Robert in a Your Skill Boost masterclass organised by the Excellence Foundation for South Sudan (EFSS) on Saturday, the 25th and Sunday, the 26th of May 2024.
He discussed the concept of quality improvement, emphasizing its applicability to various aspects of life, including personal, project, and program improvements. He defined quality as doing the right thing at the right time in the right way to achieve the best possible results and discussed the concept of the "gap" between what we know and what we do, and how this gap represents the areas we need to improve. He explained the scientific approach to quality improvement, which involves systematic performance analysis, testing and learning, and implementing change ideas. He also highlighted the importance of client focus and a team approach to quality improvement.
GAS CHROMATOGRAPHY AND MASS SPECTROMETRY (GC-MS) BY P.RAVISANKAR.
1.
2. The use of a mass spectrometer as the detector
in gas chromatography was developed during
the 1950s by Roland Gohlke and Fred
McLafferty.
GC = separation
MS = identification
When GC is combined with MS, a powerful
analytical tool is created.
3.
4. Desired characters:
To be suitable for GC analysis, a compound must
have sufficient volatility and thermal stability.
(All or some of compound molecules are in the gas
or vapor phase at 250-350 C or below, do not
decompose at these temperatures.)
Organic compounds must be in solution for injection
into the gas chromatograph. The solvent must be
volatile and organic (for example, hexane or
dichloromethane).
GAS CHROMATOGRAPHY
5. PRINCIPLE:
The principle of separation in GC is partition.
Gas is used as a mobile phase & liquid which is coated
on a solid support is used as a stationary phase.
The component which is more soluble in stationary
phase travels slower & eluted later:
Hence the components are separated according to their
partition coefficient.
6. Injection port – One micro liter ( 0.000001 L) of
solvent containing the mixture of molecules is
injected into the GC and the sample is carried by inert
(non-reactive) gas through the instrument, usually
helium. The inject port is heated to 300 C to cause
the chemicals to become gases.
Column – column is one of the important part of GC
which decides the separation efficiency. In GCMS
support coated open tubular columns are mostly
preferred
INSTRUMENTATION
7. Incompatibility of GC and MS
GC operate at atmospheric pressure and the MS ion source
at 10-5 Torr.
108 fold pressure difference.
Need of the interface.
The carrier gas must be removed and GC peak components
transferred to the MS ion source.
10. PRINCIPLE:-
Mass spectra is also called positive ion spectra.
In this electron bombardment is used to convert a neutral
molecule in to positively charged one.
Obtaining mass spectra consists 2 steps:
Conversion of neutral molecule in to positively charged
one.
Separation of positively charged fragments formed based
upon their masses using electrical & magnetic field:
11. Ion Source – After passing through the GC, the
chemical pulses continue to the MS. The sample
molecules must be ionized& converted in to
charged particles by the ion source before they
can be analyzed. The most commonly used
method for this purpose are :
Electron ionization.
Chemical ionization.
12. In GCMS QUADRAPO-LE ION TRAP mass analyzer is
preferred
Mass anylyzer--sample has been ionized the beam of ions is
accelerated by an electric field& then passes into the mass
analyzer, the region of the mass spectrophotometer where the
ions are separated according to their m/z ratio.
13. Detector – A detector counts the number of ions with a
specific mass. The mass spectrum is a graph of the number of
ions with different masses that traveled through the mass
analyzer. ELECTRON MULTIPLIER CELL is the detector
used in GCMS
14. The GC works on the principle that a mixture will
separate into individual substances when heated.
Sample introduced into GC inlet vaporized at 250 C
, swept onto the column by He carrier gas &
separated on column.
Sample components emerge from column, flowing
into the capillary column interface connecting the
GC column and the MS (He removed).
Identification of a compound based on it's mass
spectrum relies on the fact that every compound
has a unique fragmentation pattern.
HOW GC-MS WORKS
15. The computer drives the MS, records the data, and
converts the electrical impulses into visual displays and
hard copy displays.
As each solute exits the GC column, it is diverted into a
mass spectrometer which is capable of both monitoring
the amount of and identifying the chemical nature of the
solute.
In this way, both quantitative and qualitative information
about the mixture can be obtained.
The sequence and relative intensity of the mass peaks
give information about the chemical identity of the solute.
The absolute intensity of the peaks provides information
about the amount of substance present.
18. Inject the specimen into the septum rapidly and
smoothly to attain good separation of the components in
a specimen.
If the technician injects the specimen too slowly, the
peak may be broad or overlap. A twin peak may result
from the technician hesitating during the injection.
.
19. Through GC – a chromatogram is obtained.
Through MS – a spectrum is obtained.
GC-MS gives a 3D graph which has both
chromatogram and a spectrum to each separated
component in chromatogram.
20. While the instrument
runs, the computer
generates a graph
from the signal. This
graph is called a
chromatogram.
Each of the peaks in
the chromatogram
represents the signal
created when a
compound elutes
from the GC column
into the detector.
x-axis shows the RT
y-axis shows the
intensity (abundance)
of the signal.
21. The computer records a
graph for each scan.
This graph is referred
to as a mass spectrum.
The mass spectrum is
essentially a fingerprint
for the molecule and
can be used to identify
the compound.
The library compares
the mass spectrum
from a sample
component and
compares it to mass
spectra in the library.
22. It has both the chromatogram and spectrum
on Y-axis it represents intensity/abundance, on X-axis it is
retention time.
The graph between retention time and abundance is a
chromatogram.
The graph between m/z and retention time is mass
spectrum of the individual peaks of the chromatogram.
23. Environmental Monitoring and
Cleanup
Criminal Forensics
Law Enforcement
Security
Food, Beverage and Perfume Analysis
Medicine
24. To analyze small molecule metabolite identifications in
diabetic versus non diabetic urine samples
Analysis of Anabolic Steroids in Biological materials.
Quantitation of pollutants in drinking and wastewater
using official U.S. Environmental Protection Agency (EPA)
methods.
Quantitation of drugs and their metabolites in blood and
urine for both pharmacological and forensic.
Determination of furans in food beverages
25. The separation and identification of degradation
products of organic and organometallics making the
elucidation of their structures.
The routine analysis of substances present in minute
quantities
The identification of noxious and toxic compounds
and their quantitation in emergency cases.
The diagnosis of inborn errors of metabolism
especially in new borns
Analysis of butylated hydroxytoulene in food
26. General
Only compounds with vapor pressures exceeding
about 10 torr
Many compounds with lower pressures can be
analyzed if they are chemically derivatized .
Determining positional substitution on aromatic
rings is often difficult.
Certain isomeric compounds cannot be
distinguished by mass spectrometry.
27. Dow gas chromatography and Bendix TOF mass
spectrometer in the Dow Spectroscopy Laboratory, 1957.
29. Instrumental methods of chemical analysis, Gurudeep R
Chatwal, Sham K. Anand , Pages 2.699
Practical Pharmaceutical Chemistry fourth edition-Part
two, Edited by A.H.Beckett, J.B.Stenlake, Pages 474-477
Gas Chromatography Mass Spectrometry, Ronald A.
Hites, Indiana University School of Public and
Environmental Affairs and Department of Chemistry,
Chapter 31, Pages 609-626
Journal of the American Society for Mass Spectrometry
Volume 4, Issue 5, May 1993, Pages 367-371
http://www.labcompare.com/Mass-Spectrometry/154-
Gas-Chromatograph-Mass-Spectrometer-GC-MS-
Instrument/
http://www.chem.agilent.com/enUS/Products/Instrumen
ts/ms/7000triplequadrupolegcms/Pages/default.aspx
http://www.chemistry.nmsu.edu/Instrumentation/GC_M
S.html