GC-MS is an analytical technique that combines gas chromatography and mass spectrometry to identify substances within a sample. It works by separating compounds via gas chromatography and then using mass spectrometry to determine the molecular mass of each separated component, allowing for identification. This provides both qualitative and quantitative analysis, producing a unique fingerprint spectrum for each compound to facilitate structural analysis.
Gas chromatography-mass spectrometry (GC MS) is an analytical method in which GC is coupled with MS to identify different substances within a test sample.
GCMS & LCMS
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Sub :- Advanced Analytical Techniques
M.Pharmacy Sem1
Savitribai Phule Pune University
Contents :-
GC-MS
Introduction
Principle
Instrumentation
Application
LC-MS
Introduction
Principle
Instrumentation
Application
Introduction to Gas chromatography-Mass spectroscopy
Gas chromatography-Mass spectroscopy is one of the so-called hyphenated analytical techniques. It is actually two techniques that are combined to form a single method of analyzing mixtures of chemicals
GC-MS is an instrumental technique, comprising a gas chromatograph coupled to a mass spectrometer by which complex mixtures of chemicals may be separated, identified & quantified. In order to a compound to be analysed by GC-MS it must be sufficiently volatile & thermally stable.
Principle :-
The Sample solution is injected into the GC inlet where it is vapourized & swept onto a chromatographic column by the carrier gas ( usually helium). The sample flows through the column & compounds comprising the mixture of interest are separated by virtue of their relative interaction with the coating of the column (stationery phase) & the carrier gas (mobile phase). The later part of the column passes through a heated transfer line & ends at the entrance to ion source where compounds eluting from the column are converted to ions
Gas chromatography-mass spectrometry (GC MS) is an analytical method in which GC is coupled with MS to identify different substances within a test sample.
GCMS & LCMS
htps://youtube.com/vishalshelke99
https://instagram.com/vishal_stagram
Sub :- Advanced Analytical Techniques
M.Pharmacy Sem1
Savitribai Phule Pune University
Contents :-
GC-MS
Introduction
Principle
Instrumentation
Application
LC-MS
Introduction
Principle
Instrumentation
Application
Introduction to Gas chromatography-Mass spectroscopy
Gas chromatography-Mass spectroscopy is one of the so-called hyphenated analytical techniques. It is actually two techniques that are combined to form a single method of analyzing mixtures of chemicals
GC-MS is an instrumental technique, comprising a gas chromatograph coupled to a mass spectrometer by which complex mixtures of chemicals may be separated, identified & quantified. In order to a compound to be analysed by GC-MS it must be sufficiently volatile & thermally stable.
Principle :-
The Sample solution is injected into the GC inlet where it is vapourized & swept onto a chromatographic column by the carrier gas ( usually helium). The sample flows through the column & compounds comprising the mixture of interest are separated by virtue of their relative interaction with the coating of the column (stationery phase) & the carrier gas (mobile phase). The later part of the column passes through a heated transfer line & ends at the entrance to ion source where compounds eluting from the column are converted to ions
GAS CHROMATOGRAPHY-MASS SPECTROSCOPY [GC-MS]Shikha Popali
THIS PRESENTATION GIVES A DETAIL ACCOUNT ON THE GC-MS WITH ITS INTRODUCTION, BASIC PRINCIPLE OF BOTH COMBINED AND INDIVIDUALLY WITH ITS INSTRUMENTATION, APPLICATION AND EXAMPLES, MAKES EASY TO COLLECT ALL THE DATA AT A PLACE ACCORDING TO THE M.PHARM SYLLABUS S PER PCI
Gas chromatography-mass spectrometry (GC-MS) is the synergistic combination of two analytical method to separate and identify different substances within a test sample.
Gas chromatography separates the components of a mixture in time.
Mass spectrometer provides information that aids in the identification and structural elucidation of each component.
1. It is one of the type of Hyphenated technique.
2. It is a combination of gas chromatographic technique and spectroscopic technique.
3. It is having a high resolution capacity.
4. It is used has volatile and Non-volatile compounds.
5. It is used for qualitative and quantitative analysis.
Gas chromatography-Mass spectrometry (GC-MS)Saira Fatima
PRESENTED BY
SAIRA FATIMA
SABAHAT MEHMOOD
SANA USMAN
MSc 4 (2018-2020)
Department of MicroBiology & Molecular Genetics
University of the Punjab
Lahore, Pakistan
This ppt consist of basic principle of GC-MS, instrumentation of GC-MS, components of GC-MS ,Advantages and disadvantages of GC-MS and application of GC-MS
Gas Chromatography-Mass Spectrometry (GC-MS) is an analytical method that combines the features of gas-liquid chromatography and mass spectrometry to identify different substances within a test sample.
GAS CHROMATOGRAPHY-MASS SPECTROSCOPY [GC-MS]Shikha Popali
THIS PRESENTATION GIVES A DETAIL ACCOUNT ON THE GC-MS WITH ITS INTRODUCTION, BASIC PRINCIPLE OF BOTH COMBINED AND INDIVIDUALLY WITH ITS INSTRUMENTATION, APPLICATION AND EXAMPLES, MAKES EASY TO COLLECT ALL THE DATA AT A PLACE ACCORDING TO THE M.PHARM SYLLABUS S PER PCI
Gas chromatography-mass spectrometry (GC-MS) is the synergistic combination of two analytical method to separate and identify different substances within a test sample.
Gas chromatography separates the components of a mixture in time.
Mass spectrometer provides information that aids in the identification and structural elucidation of each component.
1. It is one of the type of Hyphenated technique.
2. It is a combination of gas chromatographic technique and spectroscopic technique.
3. It is having a high resolution capacity.
4. It is used has volatile and Non-volatile compounds.
5. It is used for qualitative and quantitative analysis.
Gas chromatography-Mass spectrometry (GC-MS)Saira Fatima
PRESENTED BY
SAIRA FATIMA
SABAHAT MEHMOOD
SANA USMAN
MSc 4 (2018-2020)
Department of MicroBiology & Molecular Genetics
University of the Punjab
Lahore, Pakistan
This ppt consist of basic principle of GC-MS, instrumentation of GC-MS, components of GC-MS ,Advantages and disadvantages of GC-MS and application of GC-MS
Gas Chromatography-Mass Spectrometry (GC-MS) is an analytical method that combines the features of gas-liquid chromatography and mass spectrometry to identify different substances within a test sample.
The aim of the coupling is to obtain an information-rich detection for both identification and quantification compared to that with a single analytical technique.
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3. CONTENT
Introduction
Gas chromatography
Mass spectrometry
MS as an detector
interfacing GC with MS
Interface & its types
how GC/MS works
5. INTRODUCTION
GS-MS is an Advance analytical instrumental technique that combines the physical
separation capability of GC with the mass analysing capability of MS.
It is very versatile tool to separates ,quantify and identify unknown substances.
GC separates the components of mixture.
MS provides information that aids in the structural identification of each
component.
7. GAS CHROMATOGRAPHY
Used to separates the components of mixture.
The principle of separation in GC is partition or adsorption depend upon which type
of stationary phase used.
In gas chromatography, the sample is injected and vaporized in the injection port.
Elution is brought about by the flow of an inert gaseous mobile phase.
The mobile phase does not interact with molecule of the analyte;its only function is to
transport the analyses through the column.
Retention times are based on the polarity of the substance compared to the column.
8.
9. Mass spectrometry
It is a technique used for measuring the molecular weight and determining the
molecular formula.
The spectra that obtained is called positive ion spectra .
Apparatus
1. Sample inlet system
2. Ionization source
3. Ion accelerator chamber
4. Mass analyzer
5. Detector and readout
10. Principle and working
In this technique ions are produce ,separate and detecte on the basis of
their mass to charge ratio.
Sample Inlet system Convert the sample into vapours form either the
sample is liquid or solid.
Ionization chamber produce ions by bombardment of high electron from
tungsten filament.The energy of the electron(70eV) cleave a typical bond
In the ion Accelerator chamber the ions are gain some specific velocity
and move to the mass analyzer.
11. Cont...
Mass analyzer deflects the ions down a curved tube in a magnetic field based
on their kinetic energy determined by the mass ,charge and velocity .
Then these ions fall on the detector according to the their mass to charge
ratio and velocity.then readout device show the spectrum of analyte that is
represented by mass spectrum
Mass spectrum is essentially a fingerprint for the molecule and can be used to
identify the compound
13. Mass spectrometer as a detector
The mass spectrometer is an universal detector for gas
chromatography,since any compound that can pass through a
gas chromatograph is converted into ions in the mass
spectrometer. Gas chromatography is an ideal separator ,
whereas mass spectrometry is excellent for identification.
15. Interfacing gas chromatography
with mass spectrometry
The aim of an interfacing arrangement is to operate both techniques without
degrading the performance of either instrument .
Problems
Incompatibility
GC operate at atmospheric presssure and the MS ion source at 10^-5 torr.
Presence of much carrier gas and little sample in the elluent from the gas
chromatograph .
16. Interface
A device to connect two techniques
Transport the effluent from the gas chromatograph to the mass
spectrometer
Analyte must not condense in interface
Analyte may not decompose before entering the mass spectrometer
It is play an important role in the overall efficiency of the instrument
It remove the pressure incompatibility problem
17. Types of interface
direct interface
Jet separator
Watson –biemann effusion separator
18. Direct interface
Today most GC-MS system use capillary columns & fused
silica tubing permits an inert, high efficiency ,direct transfer
between the 2 systems .
Flow rate is 2ml /min.
19. Jet separators(packed column)
The separator consists of two glass tubes aligned with a small distance
between them .
Carrier gas entering from the GC column is pumped away by a separate
vacumed system.
The larger sample molecules maintain thei velocity and pass preferentially in
to the second jet.
Sample enrichment occurs & the initial atmospheric pressure is reduced.
20.
21. Watson –biemann effusion separator
It consists of sintered glass tube .
The carrier usually helium ,passes
preferentially through the sintered
glass tube , hence the analyte
enirchment occurs.
22. How GC Ms works
The GC woks on the principle that a mixture will separate into
individual when heated .
Sample introduced into GC inlet vaporized ,swept onto the column by
He carrier gas & separated on column .
Sample components emerge from column ,flowing into the capillary
column interface connecting the GC column and the MS.
Identification of a compound based on its mass spectrum relies on the
fact that every compound has a unique fragmentation pattern.
23. Cont...
The computer drives the MS , records the data , and converts the electrical
impulses into visual displays and hard copy displays.
As each solute exits the GC column , it is diverted into a mass spectrometer which
is capable of both monitoring the amount of and identifying the chemical nature
of the solute. In this way , both quantitative and qualitative information about the
mixture can be obtained .
The sequence and relative intensity of the mass peaks give information about the
chemical identity of the solute .the absolute intensity of the peaks provides
information about the amount of solute
24. Interpretation of the result
Through GC –a chromatogram is obtained
Through MS-a spectrum is obtained .
GC MS gives a 3D graph which has both chromatogram and a
spectrum to each separated component in chromatogram.
27. Advantages
Easy to use
Wide range of stationary phase
Huge spectral libraries in the instrument that help with proper identifiation
Used by many organization to monitor organic pollutant in environment.
28. Disadvantages/limitations
It is expensive to buy a GC/MS machine
It is difficult to measure and inject such small samples (approx 0.3 microlitres)
Only compound with vapour pressure exceeding about 10 torr
Determining positional substitution on aromatic ring is often difficult
Certain isomeric compound cannot be distinguish by mass spectrometery.
29. General Applications
Analysis of medicine
Food, beverage & perfume analysis
Criminal forensics
Characterization & comparison of plant extract
Envronmental monitoring
30. Pharmaceutical Applications
In the pharmaceutical industry, GC-MS is used in
Research and Development
Production and Quality control
Identification of impurities in active pharmaceutical ingredients
In medicinal chemistry
In the synthesis and characterization of compounds
In pharmaceutical biotechnology