Define DNA fingerprint and DNA fingerprinting.
Explain some terms related to DNA fingerprinting.
Describe the method of collection and preservation of biological samples.
Describe the uses of DNA fingerprinting.
Describe the types of DNA fingerprinting.
Describe the steps of DNA fingerprinting.
DNA fingerprinting is a method used to identify living things based on samples of their DNA. Instead of looking at the whole sequence of a person’s DNA, these techniques look at the presence or absence of common markers that can be quickly and easily identified.
DNA profiling process, RFLP analysis, STR analysis by PCR, basic principle of dna fingerprinting, advantages and disadvantages of RFLP and STR analysis
DNA fingerprinting is a method used to identify living things based on samples of their DNA. Instead of looking at the whole sequence of a person’s DNA, these techniques look at the presence or absence of common markers that can be quickly and easily identified.
DNA profiling process, RFLP analysis, STR analysis by PCR, basic principle of dna fingerprinting, advantages and disadvantages of RFLP and STR analysis
This presentation is about DNA fingerprinting, a brief description is given about its principle, working, technique and its application with a example.
Define DNA fingerprint and DNA fingerprinting.
Explain some terms related to DNA fingerprinting.
Describe the method of collection and preservation of biological samples.
Describe the uses of DNA fingerprinting.
Describe the types of DNA fingerprinting.
Describe the steps of DNA fingerprinting.
Presented by,
Dr. Md. Mohiuddin Masum
Resident, MS Anatomy
PAY2B6
Guided by,
Prof. Dr. Shahara Khatun
DNA Fingerprinting of plants . History,procedure of DNA fingerprinting, PCR and NON PCR technique like RAPD,SSR,RELPs, application of DNA fingerprinting, advantage and disadvantage of DNA fingerprinting.
This presentation is about DNA fingerprinting, a brief description is given about its principle, working, technique and its application with a example.
Define DNA fingerprint and DNA fingerprinting.
Explain some terms related to DNA fingerprinting.
Describe the method of collection and preservation of biological samples.
Describe the uses of DNA fingerprinting.
Describe the types of DNA fingerprinting.
Describe the steps of DNA fingerprinting.
Presented by,
Dr. Md. Mohiuddin Masum
Resident, MS Anatomy
PAY2B6
Guided by,
Prof. Dr. Shahara Khatun
DNA Fingerprinting of plants . History,procedure of DNA fingerprinting, PCR and NON PCR technique like RAPD,SSR,RELPs, application of DNA fingerprinting, advantage and disadvantage of DNA fingerprinting.
Ossification (Intracartilaginous and Intramembranous)Mohiuddin Masum
This presentation includes:
* Ossification definition
* Types of ossification
* Center of ossification
* Intramembranous ossification process
* Intracartilaginous ossification process
DNA Extraction, DNA quantity-quality check & Amplicon quantity checkMohiuddin Masum
DNA Extraction using Reliaprep™ blood gDNA extraction protocol
DNA quantity-quality check using Nanodrop and
Amplicon quantity check using Fluorometer
DNA extraction procedure video YouTube link
https://www.youtube.com/watch?v=uk2H4tJUAto
Nanodrop procedure video YouTube link
https://www.youtube.com/watch?v=JlMuF0FAU1g
Fluorometer procedure video YouTube link
https://www.youtube.com/watch?v=To1vSP1bNxo
At the end of the session audience will be able to:
Explain the mechanism of different physiological processes-
Breathing
Coughing
Sneezing
Phonation
Temperature regulation
Blinking
At the end of the session the audience will be able to-
Classify muscles and their location in the body.
Connective tissue covering and different parts of skeletal muscle.
Classify different types of skeletal muscles.
Explain different functional terms related to muscle action.
Understand and discuss some principles related to skeletal muscles.
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The prostate is an exocrine gland of the male mammalian reproductive system
It is a walnut-sized gland that forms part of the male reproductive system and is located in front of the rectum and just below the urinary bladder
Function is to store and secrete a clear, slightly alkaline fluid that constitutes 10-30% of the volume of the seminal fluid that along with the spermatozoa, constitutes semen
A healthy human prostate measures (4cm-vertical, by 3cm-horizontal, 2cm ant-post ).
It surrounds the urethra just below the urinary bladder. It has anterior, median, posterior and two lateral lobes
It’s work is regulated by androgens which are responsible for male sex characteristics
Generalised disease of the prostate due to hormonal derangement which leads to non malignant enlargement of the gland (increase in the number of epithelial cells and stromal tissue)to cause compression of the urethra leading to symptoms (LUTS
Flu Vaccine Alert in Bangalore Karnatakaaddon Scans
As flu season approaches, health officials in Bangalore, Karnataka, are urging residents to get their flu vaccinations. The seasonal flu, while common, can lead to severe health complications, particularly for vulnerable populations such as young children, the elderly, and those with underlying health conditions.
Dr. Vidisha Kumari, a leading epidemiologist in Bangalore, emphasizes the importance of getting vaccinated. "The flu vaccine is our best defense against the influenza virus. It not only protects individuals but also helps prevent the spread of the virus in our communities," he says.
This year, the flu season is expected to coincide with a potential increase in other respiratory illnesses. The Karnataka Health Department has launched an awareness campaign highlighting the significance of flu vaccinations. They have set up multiple vaccination centers across Bangalore, making it convenient for residents to receive their shots.
To encourage widespread vaccination, the government is also collaborating with local schools, workplaces, and community centers to facilitate vaccination drives. Special attention is being given to ensuring that the vaccine is accessible to all, including marginalized communities who may have limited access to healthcare.
Residents are reminded that the flu vaccine is safe and effective. Common side effects are mild and may include soreness at the injection site, mild fever, or muscle aches. These side effects are generally short-lived and far less severe than the flu itself.
Healthcare providers are also stressing the importance of continuing COVID-19 precautions. Wearing masks, practicing good hand hygiene, and maintaining social distancing are still crucial, especially in crowded places.
Protect yourself and your loved ones by getting vaccinated. Together, we can help keep Bangalore healthy and safe this flu season. For more information on vaccination centers and schedules, residents can visit the Karnataka Health Department’s official website or follow their social media pages.
Stay informed, stay safe, and get your flu shot today!
Report Back from SGO 2024: What’s the Latest in Cervical Cancer?bkling
Are you curious about what’s new in cervical cancer research or unsure what the findings mean? Join Dr. Emily Ko, a gynecologic oncologist at Penn Medicine, to learn about the latest updates from the Society of Gynecologic Oncology (SGO) 2024 Annual Meeting on Women’s Cancer. Dr. Ko will discuss what the research presented at the conference means for you and answer your questions about the new developments.
Title: Sense of Smell
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the primary categories of smells and the concept of odor blindness.
Explain the structure and location of the olfactory membrane and mucosa, including the types and roles of cells involved in olfaction.
Describe the pathway and mechanisms of olfactory signal transmission from the olfactory receptors to the brain.
Illustrate the biochemical cascade triggered by odorant binding to olfactory receptors, including the role of G-proteins and second messengers in generating an action potential.
Identify different types of olfactory disorders such as anosmia, hyposmia, hyperosmia, and dysosmia, including their potential causes.
Key Topics:
Olfactory Genes:
3% of the human genome accounts for olfactory genes.
400 genes for odorant receptors.
Olfactory Membrane:
Located in the superior part of the nasal cavity.
Medially: Folds downward along the superior septum.
Laterally: Folds over the superior turbinate and upper surface of the middle turbinate.
Total surface area: 5-10 square centimeters.
Olfactory Mucosa:
Olfactory Cells: Bipolar nerve cells derived from the CNS (100 million), with 4-25 olfactory cilia per cell.
Sustentacular Cells: Produce mucus and maintain ionic and molecular environment.
Basal Cells: Replace worn-out olfactory cells with an average lifespan of 1-2 months.
Bowman’s Gland: Secretes mucus.
Stimulation of Olfactory Cells:
Odorant dissolves in mucus and attaches to receptors on olfactory cilia.
Involves a cascade effect through G-proteins and second messengers, leading to depolarization and action potential generation in the olfactory nerve.
Quality of a Good Odorant:
Small (3-20 Carbon atoms), volatile, water-soluble, and lipid-soluble.
Facilitated by odorant-binding proteins in mucus.
Membrane Potential and Action Potential:
Resting membrane potential: -55mV.
Action potential frequency in the olfactory nerve increases with odorant strength.
Adaptation Towards the Sense of Smell:
Rapid adaptation within the first second, with further slow adaptation.
Psychological adaptation greater than receptor adaptation, involving feedback inhibition from the central nervous system.
Primary Sensations of Smell:
Camphoraceous, Musky, Floral, Pepperminty, Ethereal, Pungent, Putrid.
Odor Detection Threshold:
Examples: Hydrogen sulfide (0.0005 ppm), Methyl-mercaptan (0.002 ppm).
Some toxic substances are odorless at lethal concentrations.
Characteristics of Smell:
Odor blindness for single substances due to lack of appropriate receptor protein.
Behavioral and emotional influences of smell.
Transmission of Olfactory Signals:
From olfactory cells to glomeruli in the olfactory bulb, involving lateral inhibition.
Primitive, less old, and new olfactory systems with different path
These lecture slides, by Dr Sidra Arshad, offer a quick overview of physiological basis of a normal electrocardiogram.
Learning objectives:
1. Define an electrocardiogram (ECG) and electrocardiography
2. Describe how dipoles generated by the heart produce the waveforms of the ECG
3. Describe the components of a normal electrocardiogram of a typical bipolar leads (limb II)
4. Differentiate between intervals and segments
5. Enlist some common indications for obtaining an ECG
Study Resources:
1. Chapter 11, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 9, Human Physiology - From Cells to Systems, Lauralee Sherwood, 9th edition
3. Chapter 29, Ganong’s Review of Medical Physiology, 26th edition
4. Electrocardiogram, StatPearls - https://www.ncbi.nlm.nih.gov/books/NBK549803/
5. ECG in Medical Practice by ABM Abdullah, 4th edition
6. ECG Basics, http://www.nataliescasebook.com/tag/e-c-g-basics
ARTIFICIAL INTELLIGENCE IN HEALTHCARE.pdfAnujkumaranit
Artificial intelligence (AI) refers to the simulation of human intelligence processes by machines, especially computer systems. It encompasses tasks such as learning, reasoning, problem-solving, perception, and language understanding. AI technologies are revolutionizing various fields, from healthcare to finance, by enabling machines to perform tasks that typically require human intelligence.
New Directions in Targeted Therapeutic Approaches for Older Adults With Mantl...i3 Health
i3 Health is pleased to make the speaker slides from this activity available for use as a non-accredited self-study or teaching resource.
This slide deck presented by Dr. Kami Maddocks, Professor-Clinical in the Division of Hematology and
Associate Division Director for Ambulatory Operations
The Ohio State University Comprehensive Cancer Center, will provide insight into new directions in targeted therapeutic approaches for older adults with mantle cell lymphoma.
STATEMENT OF NEED
Mantle cell lymphoma (MCL) is a rare, aggressive B-cell non-Hodgkin lymphoma (NHL) accounting for 5% to 7% of all lymphomas. Its prognosis ranges from indolent disease that does not require treatment for years to very aggressive disease, which is associated with poor survival (Silkenstedt et al, 2021). Typically, MCL is diagnosed at advanced stage and in older patients who cannot tolerate intensive therapy (NCCN, 2022). Although recent advances have slightly increased remission rates, recurrence and relapse remain very common, leading to a median overall survival between 3 and 6 years (LLS, 2021). Though there are several effective options, progress is still needed towards establishing an accepted frontline approach for MCL (Castellino et al, 2022). Treatment selection and management of MCL are complicated by the heterogeneity of prognosis, advanced age and comorbidities of patients, and lack of an established standard approach for treatment, making it vital that clinicians be familiar with the latest research and advances in this area. In this activity chaired by Michael Wang, MD, Professor in the Department of Lymphoma & Myeloma at MD Anderson Cancer Center, expert faculty will discuss prognostic factors informing treatment, the promising results of recent trials in new therapeutic approaches, and the implications of treatment resistance in therapeutic selection for MCL.
Target Audience
Hematology/oncology fellows, attending faculty, and other health care professionals involved in the treatment of patients with mantle cell lymphoma (MCL).
Learning Objectives
1.) Identify clinical and biological prognostic factors that can guide treatment decision making for older adults with MCL
2.) Evaluate emerging data on targeted therapeutic approaches for treatment-naive and relapsed/refractory MCL and their applicability to older adults
3.) Assess mechanisms of resistance to targeted therapies for MCL and their implications for treatment selection
1. DNA Fingerprinting
Dr. Md. Mohiuddin Masum
Bangabandhu Sheikh Mujib Medical University
Dhaka, Bangladesh.
2.
3.
4. Define DNA fingerprint and DNA fingerprinting
Explain some terms related to DNA fingerprinting
Describe the method of collection and preservation of
biological samples
Describe the uses of DNA fingerprinting
Describe the types of DNA fingerprinting
Describe the steps of DNA fingerprinting
Objectives
5.
6. A small set of DNA variation
that is very likely to be different
in all unrelated individuals,
thereby being as unique to individuals
as are fingerprint
DNA Fingerprint
7. A method used to identify an individual from a
sample of DNA
by looking at unique patterns
in their DNA sequence
DNA Fingerprinting
www.yourgenome.org
8. Also known as,
DNA Fingerprinting
DNA profiling
DNA testing
DNA typing
Genetic fingerprinting
13. Terms related to DNA fingerprinting
Polymorphism
http://groups.molbiosci.northwestern.edu//DNA_polymorphism
14. Terms related to DNA fingerprinting
DNA Polymorphism
http://groups.molbiosci.northwestern.edu//DNA_polymorphism
Single nucleotide polymorphism (SNP)
Minisatellite or variable number of tandem repeat
(VNTR)
Microsatellite or short tandem repeat (STR)
15. Terms related to DNA fingerprinting
Junk DNA
Proteomics & Genomics/Dr. Vikash Kumar Dubey
95%
16. Terms related to DNA fingerprinting
Tandem repeat
www.bio.miami.edu/dana/dox/vntr.html
18. Terms related to DNA fingerprinting
Minisatellite or VNTR
AGTTCGCGTGAAGTTCGCGTGAAGTTCGCGTGA
https://en.wikipedia.org/wiki/Variable_number_tandem_repeat
19. Terms related to DNA fingerprinting
Microsatellite or STR
ATGCCATGCCATGCCATGCCATGCC
https://en.wikipedia.org/wiki/Microsatellite
20. Terms related to DNA fingerprinting
Restriction endonuclease
Escheria coli EcoRI
5’ GAATTC 3’
3’ CTTAAG 5’
5’ G AATTC 3’
3’ CTTAA G 5’
https://en.wikipedia.org/wiki/Restriction_enzyme
21. Blood
Saliva
Semen
Tissue from personal item
From stored sample
Hair follicle
Sources of DNA evidence
33. Restriction fragment length
polymorphism (RFLP)
Polymerase chain reaction amplification
of short tandem repeat (PCR/STR)
Types of DNA fingerprinting
34. RFLP
Types of DNA fingerprinting
DNA
extraction
Restriction
digestion
Electrophoresis
Transfer of DNA to
membrane
Hybridization of
DNA
X-ray
38. 13 CODIS core STR loci
with chromosomal position
Types of DNA fingerprinting
39. Types of DNA fingerprinting
Restriction fragment length
polymorphism (RFLP)
Polymerase chain reaction
amplification of short tandem
repeats (PCR/SRT)
More sample needed Less sample needed
Fresh DNA sample needed Fresh DNA sample not always
mandatory
No chance of amplification of
contamination
Chance amplification of
contamination
Require more time Require less time
Analysis is costly. Analysis is cheap than RFLP
Conventional fingerprint of an individual comes from finger tip and unique for an individual. This is used for identification of a person in forensic lab, police station etc. However, the major drawback of the conventional fingerprints is that it can be changed by surgery. There is another type of fingerprint unique to an individual called DNA fingerprint. This remains same in all body parts, tissues and cells as well as cannot be altered by any known methods. Thus, DNA fingerprint method is becoming primary method for identifying an individual.
Conventional fingerprint of an individual comes from finger tip and unique for an individual. This is used for identification of a person in forensic lab, police station etc. However, the major drawback of the conventional fingerprints is that it can be changed by surgery. There is another type of fingerprint unique to an individual called DNA fingerprint. This remains same in all body parts, tissues and cells as well as cannot be altered by any known methods. Thus, DNA fingerprint method is becoming primary method for identifying an individual.
The process of DNA fingerprinting was invented by Sir Alec Jeffrey at the University of Leicester in 1984
the first case (March 1985) was not strictly a forensic case but one of immigration. The first application of DNA fingerprinting saved a young boy from deportation.
Colin Pitchfork was the first criminal caught based on DNA fingerprinting evidence. He was arrested in 1986 for the rape and murder of two girls and was sentenced in 1988.
To compare the victim’s or suspect’s DNA profile to the recovered crime-scene DNA, the laboratory will need to have their known biological samples available for a side-by-side comparison. These known samples are called reference samples.
The DNA profiling of each individual is unique because of the diverse in polymorphic regions present in genome of every individual. These polymorphic regions used for identification are the non-coding regions of the genome. The polymorphic regions of the DNA do not code for proteins and which make-up 95% of our genetic DNA. Hence these regions are therefore called the ―junk DNA‖. Although these ―junk DNA‖ regions do not code for proteins, they are involved in regulating gene expression, they help in reading of other genes that code for protein and are a large portion of the chromosome structure.
Best sample to take from a dead body for DNA testing:
Blood, tissue or hair roots can be collected from a body.
If the body is decomposed, the best samples are long bones such as the humerus or femur.
However, we can also work with teeth.
METHODS OF COLLECTION:
· Whole blood Sample: Sterile needle should be used while withdrawing or collecting blood.
· Blood stain: Should be picked up preferably on sterile cotton gauge using sterile forceps and blade.
· Seminal stain: Should not be touched by hand especially the stain portion. Should be picked up with sterile forceps.
· Hard Tissues: Bones-- bones should be picked up using gloves, Kept at a place where there are no chances of environmental contamination. It should be allowed to dry completely.
· Soft Tissues: Body organs should be collected using forces and wearing gloves. It should be kept in a sterile container.
· Hair: Hair roots are preferred for the analysis. Hair roots should be picked up using sterile forceps.
Can you guess which one is Sara and which one is Miss Ellis?
Dr Ian Findlay at the Australian Genome Research Facility, the University of Queensland, developed the now patented technique which has the same level of accuracy achieved by traditional DNA fingerprinting methods.