DNA fingerprinting is a technique developed in 1985 by Alec Jeffreys that analyzes variations in repeated sequences of DNA to generate unique profiles for identification purposes. It involves extracting DNA from samples, cutting the DNA into fragments using restriction enzymes, separating the fragments via gel electrophoresis, and comparing banding patterns to determine if two DNA samples match. DNA fingerprinting is widely used for criminal investigations, paternity testing, and tracing the origin of food and other products.
DNA fingerprinting is a method used to identify living things based on samples of their DNA. Instead of looking at the whole sequence of a person’s DNA, these techniques look at the presence or absence of common markers that can be quickly and easily identified.
DNA fingerprinting is a method used to identify living things based on samples of their DNA. Instead of looking at the whole sequence of a person’s DNA, these techniques look at the presence or absence of common markers that can be quickly and easily identified.
Techniques of DNA Extraction, Purification and QuantificationBHUMI GAMETI
Introduction
The overall process…
Uses of isolated genomic DNA
Extraction of DNA from plant material
Components of DNA extraction solutions
Cell Lysis or Cell disruption :
Purification of DNA
CTAB Method
Phenol–chloroform extraction
PROTEINASE K
Salting out
Silica adsorption method
Magnetic beads
FTA Paper
Nucleic acid quantification
Agarose Gel Electrophoresis
UV spectroscopy
DNA quantification using NanoDrop
DNA profiling process, RFLP analysis, STR analysis by PCR, basic principle of dna fingerprinting, advantages and disadvantages of RFLP and STR analysis
DNA sequencing is a laboratory technique used to determine the exact sequence of bases (A, C, G, and T) in a DNA molecule. The DNA base sequence carries the information a cell needs to assemble protein and RNA molecules. DNA sequence information is important to scientists investigating the functions of genes.
In medicine, DNA sequencing is used for a range of purposes, including diagnosis and treatment of diseases. In general, sequencing allows health care practitioners to determine if a gene or the region that regulates a gene contains changes, called variants or mutations, that are linked to a disorder.
Sanger sequencing is a method of DNA sequencing based on the selective incorporation of chain-terminating dideoxynucleotides by DNA polymerase during in vitro DNA replication.
This presentation is about DNA fingerprinting, a brief description is given about its principle, working, technique and its application with a example.
Techniques of DNA Extraction, Purification and QuantificationBHUMI GAMETI
Introduction
The overall process…
Uses of isolated genomic DNA
Extraction of DNA from plant material
Components of DNA extraction solutions
Cell Lysis or Cell disruption :
Purification of DNA
CTAB Method
Phenol–chloroform extraction
PROTEINASE K
Salting out
Silica adsorption method
Magnetic beads
FTA Paper
Nucleic acid quantification
Agarose Gel Electrophoresis
UV spectroscopy
DNA quantification using NanoDrop
DNA profiling process, RFLP analysis, STR analysis by PCR, basic principle of dna fingerprinting, advantages and disadvantages of RFLP and STR analysis
DNA sequencing is a laboratory technique used to determine the exact sequence of bases (A, C, G, and T) in a DNA molecule. The DNA base sequence carries the information a cell needs to assemble protein and RNA molecules. DNA sequence information is important to scientists investigating the functions of genes.
In medicine, DNA sequencing is used for a range of purposes, including diagnosis and treatment of diseases. In general, sequencing allows health care practitioners to determine if a gene or the region that regulates a gene contains changes, called variants or mutations, that are linked to a disorder.
Sanger sequencing is a method of DNA sequencing based on the selective incorporation of chain-terminating dideoxynucleotides by DNA polymerase during in vitro DNA replication.
This presentation is about DNA fingerprinting, a brief description is given about its principle, working, technique and its application with a example.
To describe DNA extraction
To explain and demonstrate DNA cloning
To explain the process of PCR and its uses.
To explain DNA fingerprinting and its uses
DNA Fingerprinting of plants . History,procedure of DNA fingerprinting, PCR and NON PCR technique like RAPD,SSR,RELPs, application of DNA fingerprinting, advantage and disadvantage of DNA fingerprinting.
CONTENT
-Hybridization Introduction
-Southern Hybridization
Blotting (Transfer Of DNA On A Membrane)
-Application
-DNA Fingerprinting
-DNA Typing
Introduction-Hybridization
The capacity of denatured DNA to reanneal under appropriate ionic strength and temperature is known as Hybridization.
In case of a mixture of DNA molecules, hybridization leads to formation of hybrid molecules.
Often a DNA probe is used in hybridization for detecting or finding a particular DNA sequence from a mixture of DNA molecules.
Hybridization may be done with DNA fragments separated by electrophoresis, dot blot: DNA spots on a membrane or colony blot: hybridization with bacterial colonies containing particular rDNA.
Southern Hybridization
A method of DNA hybridization established by Ed Southern in 1975.
Genomic DNA from an organism is restriction digested and separated by electrophoresis.
The electropherosed DNA is transferred on to a membrane.
The membrane bound DNA is denatured using an alkali.
A DNA probe is radiolabeled and added to the denatured DNA in presence of appropriate buffer for hybridization.
After sufficient time for hybridization the membrane is exposed to X-ray film.
The X-ray film shows signals of hybridization: where the DNA probe has complementary structure on the transferred DNA.
The resulting X-ray film is known as an Autoradiogram and the process Autoradiography.
DNA fingerprinting
Using the method of DNA hybridization with an appropriate DNA probe to identify individuals as done with fingerprinting.
The method was first done by Sir Alec Jeffreys in 1985.
Any biological material such as a drop of blood, saliva, semen, and any body part such as bones, tissue, skull, teeth, hair with root etc found at the scene of crime is used as source DNA.
DNA probe used for DNA fingerprinting may be a VNTR or minisatellite DNA.
The probe is labeled and used to hybridize the source DNA.
An autoradiogram is generated that show different band patterns for different individuals.
Search and Society: Reimagining Information Access for Radical FuturesBhaskar Mitra
The field of Information retrieval (IR) is currently undergoing a transformative shift, at least partly due to the emerging applications of generative AI to information access. In this talk, we will deliberate on the sociotechnical implications of generative AI for information access. We will argue that there is both a critical necessity and an exciting opportunity for the IR community to re-center our research agendas on societal needs while dismantling the artificial separation between the work on fairness, accountability, transparency, and ethics in IR and the rest of IR research. Instead of adopting a reactionary strategy of trying to mitigate potential social harms from emerging technologies, the community should aim to proactively set the research agenda for the kinds of systems we should build inspired by diverse explicitly stated sociotechnical imaginaries. The sociotechnical imaginaries that underpin the design and development of information access technologies needs to be explicitly articulated, and we need to develop theories of change in context of these diverse perspectives. Our guiding future imaginaries must be informed by other academic fields, such as democratic theory and critical theory, and should be co-developed with social science scholars, legal scholars, civil rights and social justice activists, and artists, among others.
Connector Corner: Automate dynamic content and events by pushing a buttonDianaGray10
Here is something new! In our next Connector Corner webinar, we will demonstrate how you can use a single workflow to:
Create a campaign using Mailchimp with merge tags/fields
Send an interactive Slack channel message (using buttons)
Have the message received by managers and peers along with a test email for review
But there’s more:
In a second workflow supporting the same use case, you’ll see:
Your campaign sent to target colleagues for approval
If the “Approve” button is clicked, a Jira/Zendesk ticket is created for the marketing design team
But—if the “Reject” button is pushed, colleagues will be alerted via Slack message
Join us to learn more about this new, human-in-the-loop capability, brought to you by Integration Service connectors.
And...
Speakers:
Akshay Agnihotri, Product Manager
Charlie Greenberg, Host
Dev Dives: Train smarter, not harder – active learning and UiPath LLMs for do...UiPathCommunity
💥 Speed, accuracy, and scaling – discover the superpowers of GenAI in action with UiPath Document Understanding and Communications Mining™:
See how to accelerate model training and optimize model performance with active learning
Learn about the latest enhancements to out-of-the-box document processing – with little to no training required
Get an exclusive demo of the new family of UiPath LLMs – GenAI models specialized for processing different types of documents and messages
This is a hands-on session specifically designed for automation developers and AI enthusiasts seeking to enhance their knowledge in leveraging the latest intelligent document processing capabilities offered by UiPath.
Speakers:
👨🏫 Andras Palfi, Senior Product Manager, UiPath
👩🏫 Lenka Dulovicova, Product Program Manager, UiPath
DevOps and Testing slides at DASA ConnectKari Kakkonen
My and Rik Marselis slides at 30.5.2024 DASA Connect conference. We discuss about what is testing, then what is agile testing and finally what is Testing in DevOps. Finally we had lovely workshop with the participants trying to find out different ways to think about quality and testing in different parts of the DevOps infinity loop.
UiPath Test Automation using UiPath Test Suite series, part 3DianaGray10
Welcome to UiPath Test Automation using UiPath Test Suite series part 3. In this session, we will cover desktop automation along with UI automation.
Topics covered:
UI automation Introduction,
UI automation Sample
Desktop automation flow
Pradeep Chinnala, Senior Consultant Automation Developer @WonderBotz and UiPath MVP
Deepak Rai, Automation Practice Lead, Boundaryless Group and UiPath MVP
Kubernetes & AI - Beauty and the Beast !?! @KCD Istanbul 2024Tobias Schneck
As AI technology is pushing into IT I was wondering myself, as an “infrastructure container kubernetes guy”, how get this fancy AI technology get managed from an infrastructure operational view? Is it possible to apply our lovely cloud native principals as well? What benefit’s both technologies could bring to each other?
Let me take this questions and provide you a short journey through existing deployment models and use cases for AI software. On practical examples, we discuss what cloud/on-premise strategy we may need for applying it to our own infrastructure to get it to work from an enterprise perspective. I want to give an overview about infrastructure requirements and technologies, what could be beneficial or limiting your AI use cases in an enterprise environment. An interactive Demo will give you some insides, what approaches I got already working for real.
"Impact of front-end architecture on development cost", Viktor TurskyiFwdays
I have heard many times that architecture is not important for the front-end. Also, many times I have seen how developers implement features on the front-end just following the standard rules for a framework and think that this is enough to successfully launch the project, and then the project fails. How to prevent this and what approach to choose? I have launched dozens of complex projects and during the talk we will analyze which approaches have worked for me and which have not.
Neuro-symbolic is not enough, we need neuro-*semantic*Frank van Harmelen
Neuro-symbolic (NeSy) AI is on the rise. However, simply machine learning on just any symbolic structure is not sufficient to really harvest the gains of NeSy. These will only be gained when the symbolic structures have an actual semantics. I give an operational definition of semantics as “predictable inference”.
All of this illustrated with link prediction over knowledge graphs, but the argument is general.
Let's dive deeper into the world of ODC! Ricardo Alves (OutSystems) will join us to tell all about the new Data Fabric. After that, Sezen de Bruijn (OutSystems) will get into the details on how to best design a sturdy architecture within ODC.
Epistemic Interaction - tuning interfaces to provide information for AI supportAlan Dix
Paper presented at SYNERGY workshop at AVI 2024, Genoa, Italy. 3rd June 2024
https://alandix.com/academic/papers/synergy2024-epistemic/
As machine learning integrates deeper into human-computer interactions, the concept of epistemic interaction emerges, aiming to refine these interactions to enhance system adaptability. This approach encourages minor, intentional adjustments in user behaviour to enrich the data available for system learning. This paper introduces epistemic interaction within the context of human-system communication, illustrating how deliberate interaction design can improve system understanding and adaptation. Through concrete examples, we demonstrate the potential of epistemic interaction to significantly advance human-computer interaction by leveraging intuitive human communication strategies to inform system design and functionality, offering a novel pathway for enriching user-system engagements.
Accelerate your Kubernetes clusters with Varnish CachingThijs Feryn
A presentation about the usage and availability of Varnish on Kubernetes. This talk explores the capabilities of Varnish caching and shows how to use the Varnish Helm chart to deploy it to Kubernetes.
This presentation was delivered at K8SUG Singapore. See https://feryn.eu/presentations/accelerate-your-kubernetes-clusters-with-varnish-caching-k8sug-singapore-28-2024 for more details.
GDG Cloud Southlake #33: Boule & Rebala: Effective AppSec in SDLC using Deplo...James Anderson
Effective Application Security in Software Delivery lifecycle using Deployment Firewall and DBOM
The modern software delivery process (or the CI/CD process) includes many tools, distributed teams, open-source code, and cloud platforms. Constant focus on speed to release software to market, along with the traditional slow and manual security checks has caused gaps in continuous security as an important piece in the software supply chain. Today organizations feel more susceptible to external and internal cyber threats due to the vast attack surface in their applications supply chain and the lack of end-to-end governance and risk management.
The software team must secure its software delivery process to avoid vulnerability and security breaches. This needs to be achieved with existing tool chains and without extensive rework of the delivery processes. This talk will present strategies and techniques for providing visibility into the true risk of the existing vulnerabilities, preventing the introduction of security issues in the software, resolving vulnerabilities in production environments quickly, and capturing the deployment bill of materials (DBOM).
Speakers:
Bob Boule
Robert Boule is a technology enthusiast with PASSION for technology and making things work along with a knack for helping others understand how things work. He comes with around 20 years of solution engineering experience in application security, software continuous delivery, and SaaS platforms. He is known for his dynamic presentations in CI/CD and application security integrated in software delivery lifecycle.
Gopinath Rebala
Gopinath Rebala is the CTO of OpsMx, where he has overall responsibility for the machine learning and data processing architectures for Secure Software Delivery. Gopi also has a strong connection with our customers, leading design and architecture for strategic implementations. Gopi is a frequent speaker and well-known leader in continuous delivery and integrating security into software delivery.
Essentials of Automations: Optimizing FME Workflows with ParametersSafe Software
Are you looking to streamline your workflows and boost your projects’ efficiency? Do you find yourself searching for ways to add flexibility and control over your FME workflows? If so, you’re in the right place.
Join us for an insightful dive into the world of FME parameters, a critical element in optimizing workflow efficiency. This webinar marks the beginning of our three-part “Essentials of Automation” series. This first webinar is designed to equip you with the knowledge and skills to utilize parameters effectively: enhancing the flexibility, maintainability, and user control of your FME projects.
Here’s what you’ll gain:
- Essentials of FME Parameters: Understand the pivotal role of parameters, including Reader/Writer, Transformer, User, and FME Flow categories. Discover how they are the key to unlocking automation and optimization within your workflows.
- Practical Applications in FME Form: Delve into key user parameter types including choice, connections, and file URLs. Allow users to control how a workflow runs, making your workflows more reusable. Learn to import values and deliver the best user experience for your workflows while enhancing accuracy.
- Optimization Strategies in FME Flow: Explore the creation and strategic deployment of parameters in FME Flow, including the use of deployment and geometry parameters, to maximize workflow efficiency.
- Pro Tips for Success: Gain insights on parameterizing connections and leveraging new features like Conditional Visibility for clarity and simplicity.
We’ll wrap up with a glimpse into future webinars, followed by a Q&A session to address your specific questions surrounding this topic.
Don’t miss this opportunity to elevate your FME expertise and drive your projects to new heights of efficiency.
3. Some baSic factS
.
When the
number is On DNA sequence of
not known, nucleotide
variable, or
Is repeated again
irrelevant, it
is sometimes and again but differ
called for person to person
a variable
number When between 10 and 60 nucleotides are
tandem repeated – Mini- satellite
repeats
(VNTR) Those with fewer are known as
microsatellites or short tandem repeat
4. introduction
It is
the number of
times
that these
STRs are
repeated that
produces
Regions of chromosomes that code for
Extrons contain blocks of repeated
the variations
proteins are called introns.
nucleotides short tandem repeats
in (STRs)
individuals. Other regions that are non-coding are called
extrons.
5. introduction
.
DNA fingerprinting on
the basis of STR doesn’t
give an Individual
DNA fingerprint but able to
fingerprint determine the two
ing samples are form the
Is also same person
called "DNA fingerprinting" is a technique that
DNA allows us to see differences in DNA
profiling fragments from one person to another
in more general form.
6. HiStory of dna
fingerprinting
•Up to 1984, the only method of
establishing and authenticating
personal identification was by the
fingerprint process.
•DNA fingerprinting technique was
devised in 1985 by Alec Jeffrey at
University of Leicester in England,
while working on the sequences
within myoglobin gene.
7. Stages of DNA Fingeprinting
DNA is extracted from sample
•Each cell is surrounded by
cell membrane
•DNA is enclosed within the
nucleus
8. Stages of DNA Fingeprinting
DNA is extracted from sample
To extract DNA , most commonly used detergent is Sodium
Dodecyl Sulfate (SDS).
Digestio
n of
proteins
and
lipids
around
the cell
9. Stages of DNA Fingerprinting
DNA is extracted from sample
•DNA in the
nucleus of the cell is
molded, folded, and
protected by Proteins
•Proteinase K is
used to break up the
proteins
10. Stages of DNA Fingerprinting
Ethanol Precipitation
The DNA must be purified from the cell extract.
Ethanol lowers the effective water concentration, causing
large bio-molecules to interpenetrate and aggregate. The result
is a visible precipitate at the interface, where the ethanol is
concentrated.
DNA will rise into the alcohol layer from the cell extract
layer.
The protein and grease parts dissolve in the bottom, watery
layer
11. Stages of DNA Fingerprinting
DNA cuts with the help of Restriction
Endonuclease
DNA is cut into millions of small fragments with the help of
restriction endonucleases
Restriction enzymes chop DNA at specific sequences.
the patterns occur in different places in different individual , the
length of the fragment differs from person to person.
e.g The EcoRI restriction enzyme recognizes the following sequence
-C-A-A-T-T-G-
-G-T-T-A-A-C
12. Stages of DNA Fingerprinting
Separation of DNA fragments
•DNA fragments are separated
by electrophoresis
•DNA is negatively charged
so it is attracted to the positive
end of the gel.
Separated DNA on the basis of gel
electrophoresis
13. Stages of DNA Fingerprinting
Southern blotting
•Patterns of fragments
are transferred to a nylon
membrane by a process called
Southern blotting.
14. Stages of DNA Fingerprinting
DNA fingerprints compared
nylon or nitrocellulose (From
southern blot) sheet is placed under
X-ray film
The radioactive probes on the
DNA fragments expose the film
produces visible pattern of light
and dark bands which is unique to
each individual
Light and dark bands of DNA when nylon
film exposed to X- ray
15. Methods of DNA Fingerprinting
Restriction enzyme based
fingerprinting
•Relatively straight forward
•When genomic DNA is cleaved with particular restriction enzymes, the
resulting fragments can contain a minisatellite (VNTR) region
•gel electrophoresis separates the digested DNA into bands based on the
length of the fragments, the pattern of bands reflects the number of
repeats in a minisatellite
•each person’s DNA will yield a unique banding pattern
16. Methods of DNA Fingerprinting
PCR – based DNA fingerprinting
• By virtue of the amplification process, it is possible to begin with only
a tiny amount of DNA
• possible to fingerprint the DNA of a single cell
• Also possible to analyze old or degraded samples using PCR-based
fingerprinting
• Degraded DNA - be nearly impossible to fingerprint with restriction
analysis
17. Methods of DNA Fingerprinting
Amplified fragment Length
Polymorphism
Come in 90’s
remains attractive because of its relatively less complicated operation
and the cost-effectiveness
use of gel in its analysis phase, there are issues of bunching of the
VTRN's, causing misidentifications in the process.
18. Methods of DNA Fingerprinting
STR ( Short tandem repeats)
Mostly widely used method for comparing samples of persons
STR analyzes how many times base pairs repeat themselves on a
particular location on a strand of DNA
Has been extremely successful for use in the personal identification of
criminal suspects, paternity issues, as well as in identification of the
diseases.
19. Applications of DNA fingerprinting
Mostly used to solve crime and medical, paternity problems
•Violent murder occurred
•Rape cases
• Inheritance cases
•Immigration cases
•DNA fingerprinting also found recent applications in food
industry
•Recent application in breast cancer detection
20. Applications of DNA Fingerprinting
Crime scene
• Pattern of the
DNA profile is
compared with those
of the victim and the
suspect
• profile matches the
suspect - provides
strong evidence that
the suspect was
present at crime scene
21. Applications of DNA fingerprinting
Application in
Food
Product identification
Genetic diversity analyses
Traceability testing
22. Applications of DNA fingerprinting
Product identification.
Based on found genetic similarities or differences, a
product's identity can be established.
makes it possible to distinguish raw materials or
products from different suppliers
High-value products, such as Basmati rice and
Arabica coffee that cannot always be easily and
reliably distinguished from inferior varieties.
23. Applications of DNA fingerprinting
Genetic diversity analysis
DNA fingerprints - been applied in the genetic diversity
analysis of wine cultivars
some varieties consisted of more than one genetically distinct
variety, and that varieties that had been considered distinct were
genetically identical
Such an analysis is interesting for many crops and forms the
basis for the analysis of variations in product quality.
24. Applications of DNA fingerprinting
Traceability testing
DNA fingerprinting technology can be used for traceability
testing
comparison of a fingerprint of a cow's ear and the fingerprint
of meat products will show whether the two samples originate
from the same animal
25. Applications of DNA fingerprinting
Commercial sauerkraut
fermentations
Process available generates more salt as waste – disposable
problem
To avoid this, one has to study bacterial ecology – previous it
was studied by bio- chemical methods
But , DNA fingerprinting found application – shows more
complex behavior of bacterial viruses which produces waste
26. Applications of DNA fingerprinting
Commercial sauerkraut
fermentations
so , on the basis of these data, one can generate new
fermentation technology – which produces less salt waste and
increases the yield
27. Applications of DNA fingerprinting
Wines – use of GM yeast
and GM grapes
Scientists at the Bavarian State Institute for Viticulture
and Horticulture in Würzburg, Germany, use DNA
fingerprinting to analyze wines.
Genetically modified (GM) yeast and GM vines are
undergoing field trials
28. Applications of DNA fingerprinting
False labeling issue
Wang Jun and his group at the Chinese University of Hong
Kong used DNA fingerprinting to differentiate between Panax
ginseng (ginseng) and Panax quinquefolius (American ginseng)
price of P. quinquefolius is usually 5–10 times greater than
that of cultivated Panax ginseng
29. Applications of DNA fingerprinting
Accessions ( distinct varieties) of
plants
accessions are traditionally classified on the basis of
morphology, but it is suspected that numerous duplicates are
present
problem is increased by the fact that the same accessions are
often named differently in different countries
30. Applications of DNA fingerprinting
Accessions ( distinct varieties) of
plants
twenty-one accessions of sweet potato from the South
African genebank were compared.
RAPD and SSR techniques were used
Results show that 4 accessions which are differently named
are the genetically same – DNA fingerprinting one can use to
identify and make gene banks of world more appropriate
31. Applications of DNA fingerprinting
Lactic acid bacteria
DNA fingerprints of lactic acid bacteria were generated by
PCR
method made it possible to identify 37 isolates from raw milk,
industrial starters and yogurt
Differentiation at species, subspecies and strain level was
possible for
Lactobacillusdelbrueckii subsp. lactis, Lb.delbrueckii subsp bul
garicus and Str.thermophilus
32. Applications of DNA fingerprinting
Miscellaneous
• Fingerprinting has provided them to determine whether a
woman who had breast cancer at about as low as 10 cancer
cells
In evolutionary studies, DNA fingerprinting has even
traced the origin of all of mankind to Africa
•fingerprinting can also be used to trace plants and plant
products back to their sources
33. concluSion
.
One can use DNA fingerprinting is
DNA very useful technique to
fingerprinting solve many problems
related to crime, rape
In near era of and murder cases when
food science to very few evidences are
widen and available
know more •DNA samples must be prepared carefully
about the
products •Also , STR based method of DNA
fingerprinting is very easy and less time
consuming