Dna profiling lecture bls 209

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DNA profiling involves analyzing variable segments of DNA that do not code for proteins between individuals. These variable segments contain differing numbers of repeated sequences that provide unique DNA fingerprints. The FBI standardizes the process to analyze 13 specific loci on various chromosomes. A person inherits one version of each locus from each parent, producing 28 unique DNA fragments. DNA profiling amplifies and tags these fragments with colored primers before electrophoresis separates and identifies the fragments to generate a unique DNA profile. Applications include identifying individuals, determining genetic mutations, and sequencing whole genomes.

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Types of molecular markers (genetics) ITS ADVANTAGES AND DISADVANTAGES What is a genetic marker? RFLP: Restriction fragment length polymorphism AFLP: Amplified fragment length polymorphism RAPD: Random amplification of polymorphic DNA ISSR: Inter simple sequence repeat STR: Short tandem repeats SCAR: Sequence characterized amplified region SNP: Single nucleotide polymorphism SSR: Simple sequence repeat

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The DNA microarray is a tool used to determine whether the DNA from a particular individual contains a mutation in genes like BRCA1 and BRCA2. The chip consists of a small glass plate encased in plastic. Some companies manufacture microarrays using methods similar to those used to make computer microchips. A DNA microarray is a collection of microscopic DNA spots attached to a solid surface. Scientists use DNA microarrays to measure the expression levels of large numbers of genes simultaneously or to genotype multiple regions of a genome. Each DNA spot contains picomoles of a specific DNA sequence, known as probes. This chapter provides an overview of DNA microarrays. Microarrays are a technology in which 1000’s of nucleic acids are bound to a surface and are used to measure the relative concentration of nucleic acid sequences in a mixture via hybridization and subsequent detection of the hybridization events. We first cover the history of microarrays and the antecedent technologies that led to their development. We then discuss the methods of manufacture of microarrays and the most common biological applications. The chapter ends with a brief discussion of the limitations of microarrays and discusses how microarrays are being rapidly replaced by DNA sequencing technologies.

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