DNA Fingerprinting.ppt
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DNA fingerprinting uses variations in DNA sequences to identify individuals. It involves cutting DNA with restriction enzymes, which recognize specific sequences, producing fragments of different sizes depending on where cuts occur. These fragments are then separated via gel electrophoresis, with each individual exhibiting a unique banding pattern that can be used for identification and is more accurate than other evidence sources.
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DNA FINGERPRINTING
DNA fingerprinting is a method used to identify individuals based on variations in their DNA sequences. It involves using restriction enzymes to cut DNA into fragments at specific sequences, which are then separated by size using gel electrophoresis. The pattern of fragment sizes is unique to each individual and can be used to identify a person, determine paternity, solve criminal cases, diagnose genetic disorders, and develop cures. The document provides details on the different types of tandem repeats like satellites, minisatellites, and microsatellites that contribute to variations between individuals, as well as the basic process and applications of DNA fingerprinting.
DNA Fingerprinting
Define DNA fingerprint and DNA fingerprinting.
Explain some terms related to DNA fingerprinting.
Describe the method of collection and preservation of biological samples.
Describe the uses of DNA fingerprinting.
Describe the types of DNA fingerprinting.
Describe the steps of DNA fingerprinting.
Presented by,
Dr. Md. Mohiuddin Masum
Resident, MS Anatomy
PAY2B6
Guided by,
Prof. Dr. Shahara Khatun
Dna fingerprinting
DNA fingerprinting is a process for identifying individuals based on their unique DNA patterns. It involves isolating DNA from a sample, cutting the DNA into fragments using restriction enzymes, sorting the fragments by size through gel electrophoresis, transferring the fragments to a membrane, and using radioactive probes to detect matching fragments and produce a fingerprint pattern for identification purposes. DNA fingerprinting is widely used in forensic investigations and criminal cases to identify suspects, as well as in diagnosing genetic disorders and researching cures due to its ability to distinguish individuals based on their DNA sequences.
DNA fingerprinting
Define DNA fingerprint and DNA fingerprinting.
Explain some terms related to DNA fingerprinting.
Describe the method of collection and preservation of biological samples.
Describe the uses of DNA fingerprinting.
Describe the types of DNA fingerprinting.
Describe the steps of DNA fingerprinting.
DNA the human body recipe by slidesgo
This document is a biology project by Bhavya Yadav on DNA fingerprinting. It discusses DNA fingerprinting techniques like DNA profiling, extraction, cutting, electrophoresis, and transfer. It explains the stages of DNA fingerprinting like breaking down cells to extract DNA, cutting DNA strands, separating fragments, and analyzing patterns. The principle is that while 99.9% of human DNA is the same, the remaining 0.1% contains non-coding regions that vary between individuals and can be used for identification. DNA fingerprinting has important applications in determining lineage, personal identification, and solving crimes.
bio project.pdf
DNA fingerprinting is a powerful forensic technique that uses an individual's unique DNA sequence as identification. It has many uses like solving crimes, paternity testing, and molecular archaeology. DNA fingerprinting was initially controversial but is now widely accepted in courts. There are different types of DNA fingerprints but short tandem repeats using PCR amplification are now the most common due to being faster, cheaper, and requiring smaller DNA samples than earlier techniques like RFLPs. Proper handling of DNA evidence is crucial to avoid contamination and allow DNA fingerprints to be admitted as evidence in court.
Rflp & dna fingerprint
Restriction fragment length polymorphism (RFLP) is a technique that exploits variations in homologous DNA sequences to distinguish between individuals. It was invented in 1984 and works by digesting DNA with restriction enzymes, which cut the DNA at specific recognition sites. This produces DNA fragments of different lengths depending on the number of recognition sites. These fragments are then separated via gel electrophoresis based on their size and can be used to generate unique fingerprints to identify individuals. RFLP analysis has applications in determining genetic diseases, paternity testing, genetic mapping, and identifying carriers of disease-causing mutations.
DNA FIngerprinting.ppt
DNA profiling is a technique used to distinguish individuals using DNA samples. It was invented in 1985 by Alec Jeffreys. The process involves breaking down cells to extract DNA, cutting the DNA into fragments using restriction enzymes, separating the fragments by size using gel electrophoresis, and analyzing the pattern of fragments to obtain a DNA profile. DNA profiling is used in criminal investigations to identify suspects by comparing crime scene DNA to suspects' profiles. It is also used to determine biological relationships in cases of paternity, maternity, and inheritance disputes. Famous cases that utilized DNA profiling include identifying Colin Pitchfork as the first criminal caught this way and the O.J. Simpson murder trial.
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DNA FINGERPRINTING
DNA fingerprinting is a method used to identify individuals based on variations in their DNA sequences. It involves using restriction enzymes to cut DNA into fragments at specific sequences, which are then separated by size using gel electrophoresis. The pattern of fragment sizes is unique to each individual and can be used to identify a person, determine paternity, solve criminal cases, diagnose genetic disorders, and develop cures. The document provides details on the different types of tandem repeats like satellites, minisatellites, and microsatellites that contribute to variations between individuals, as well as the basic process and applications of DNA fingerprinting.
DNA Fingerprinting
Define DNA fingerprint and DNA fingerprinting.
Explain some terms related to DNA fingerprinting.
Describe the method of collection and preservation of biological samples.
Describe the uses of DNA fingerprinting.
Describe the types of DNA fingerprinting.
Describe the steps of DNA fingerprinting.
Presented by,
Dr. Md. Mohiuddin Masum
Resident, MS Anatomy
PAY2B6
Guided by,
Prof. Dr. Shahara Khatun
Dna fingerprinting
DNA fingerprinting is a process for identifying individuals based on their unique DNA patterns. It involves isolating DNA from a sample, cutting the DNA into fragments using restriction enzymes, sorting the fragments by size through gel electrophoresis, transferring the fragments to a membrane, and using radioactive probes to detect matching fragments and produce a fingerprint pattern for identification purposes. DNA fingerprinting is widely used in forensic investigations and criminal cases to identify suspects, as well as in diagnosing genetic disorders and researching cures due to its ability to distinguish individuals based on their DNA sequences.
DNA fingerprinting
Define DNA fingerprint and DNA fingerprinting.
Explain some terms related to DNA fingerprinting.
Describe the method of collection and preservation of biological samples.
Describe the uses of DNA fingerprinting.
Describe the types of DNA fingerprinting.
Describe the steps of DNA fingerprinting.
DNA the human body recipe by slidesgo
This document is a biology project by Bhavya Yadav on DNA fingerprinting. It discusses DNA fingerprinting techniques like DNA profiling, extraction, cutting, electrophoresis, and transfer. It explains the stages of DNA fingerprinting like breaking down cells to extract DNA, cutting DNA strands, separating fragments, and analyzing patterns. The principle is that while 99.9% of human DNA is the same, the remaining 0.1% contains non-coding regions that vary between individuals and can be used for identification. DNA fingerprinting has important applications in determining lineage, personal identification, and solving crimes.
bio project.pdf
DNA fingerprinting is a powerful forensic technique that uses an individual's unique DNA sequence as identification. It has many uses like solving crimes, paternity testing, and molecular archaeology. DNA fingerprinting was initially controversial but is now widely accepted in courts. There are different types of DNA fingerprints but short tandem repeats using PCR amplification are now the most common due to being faster, cheaper, and requiring smaller DNA samples than earlier techniques like RFLPs. Proper handling of DNA evidence is crucial to avoid contamination and allow DNA fingerprints to be admitted as evidence in court.
Rflp & dna fingerprint
Restriction fragment length polymorphism (RFLP) is a technique that exploits variations in homologous DNA sequences to distinguish between individuals. It was invented in 1984 and works by digesting DNA with restriction enzymes, which cut the DNA at specific recognition sites. This produces DNA fragments of different lengths depending on the number of recognition sites. These fragments are then separated via gel electrophoresis based on their size and can be used to generate unique fingerprints to identify individuals. RFLP analysis has applications in determining genetic diseases, paternity testing, genetic mapping, and identifying carriers of disease-causing mutations.
DNA FIngerprinting.ppt
DNA profiling is a technique used to distinguish individuals using DNA samples. It was invented in 1985 by Alec Jeffreys. The process involves breaking down cells to extract DNA, cutting the DNA into fragments using restriction enzymes, separating the fragments by size using gel electrophoresis, and analyzing the pattern of fragments to obtain a DNA profile. DNA profiling is used in criminal investigations to identify suspects by comparing crime scene DNA to suspects' profiles. It is also used to determine biological relationships in cases of paternity, maternity, and inheritance disputes. Famous cases that utilized DNA profiling include identifying Colin Pitchfork as the first criminal caught this way and the O.J. Simpson murder trial.
bio project.pptx
DNA fingerprinting is a powerful forensic technique that analyzes variations in DNA sequences to identify individuals. It works by cutting DNA into fragments of different lengths using restriction enzymes, then comparing fragment length patterns between samples. While now widely accepted, DNA fingerprinting faced initial resistance when first introduced. This document discusses the history and development of DNA fingerprinting techniques, including early methods like RFLPs and VNTRs, and more recent STR analysis using PCR amplification, which allows analysis from smaller samples and is now the standard forensic approach. Applications of DNA fingerprinting include solving criminal cases, paternity testing, and tracing ancestry.
PPTChapter 6 Molecular Basis of Inheritance G.pptx
DNA fingerprinting is a technique that uses variations in DNA sequences to distinguish individuals. It involves extracting DNA from a sample, amplifying it if needed, cutting it with restriction enzymes to isolate variable number tandem repeats, separating the fragments by size using gel electrophoresis, treating the DNA fragments with a radioactive probe, and exposing X-ray film to create an image-based fingerprint of the sample's DNA pattern. This fingerprint can then be used to identify individuals or determine familial relationships for various applications like criminal investigations, identifying remains, and tracing hereditary conditions.
PPTChapter 6 Molecular Basis of Inheritance G (1).pptx
DNA fingerprinting is a technique that uses variations in DNA sequences to distinguish individuals. It involves extracting DNA from a sample, amplifying it if needed, cutting it with restriction enzymes to isolate variable number tandem repeats, separating the fragments by size using gel electrophoresis, treating the DNA fragments with a radioactive probe, and exposing X-ray film to create an image-based fingerprint of the sample's DNA pattern. This fingerprint can then be used to identify individuals or determine familial relationships for various applications like criminal investigations, identifying remains, and tracing hereditary conditions.
Dna profiling
DNA profiling uses patterns of repeating DNA sequences that vary between individuals to identify people at a molecular level. It has various applications including criminal identification by comparing DNA evidence from a crime scene to a suspect's DNA, determining biological relationships in cases of disputed parentage, and diagnosing inherited genetic disorders. The most common current method is short tandem repeat analysis using PCR to amplify short repeating sequences and generate a DNA fingerprint.
DNA Fingerprinting
DNA fingerprinting is a technique used for identification by extracting and analyzing the base pair pattern of an individual's DNA. It involves isolating DNA from a sample, cutting the DNA into fragments using restriction enzymes, and comparing the fragment patterns on a gel to identify individuals. The main applications of DNA fingerprinting are in solving criminal cases by matching DNA evidence to suspects, diagnosing inherited diseases, and determining biological relationships in areas like paternity testing.
Rflp presentation
This document discusses Restriction Fragment Length Polymorphism (RFLP) analysis. RFLP is a technique used to detect genetic mutations and variations between individuals. It works by digesting DNA with restriction enzymes, which cut the DNA into fragments of varying lengths. These fragments are then separated via gel electrophoresis and analyzed to detect any length polymorphisms between individuals, indicating genetic differences. RFLP has applications in forensics, mutation detection, and requires isolating DNA, restriction digestion, gel electrophoresis, Southern blotting, and DNA hybridization.
Copy Of Dna Sequencing
RFLP analysis involves fragmenting DNA samples with restriction enzymes, separating the fragments by length through gel electrophoresis, and detecting length polymorphisms between individuals. Any single nucleotide change can create or destroy a restriction site, altering the length of the resulting fragment. RFLP analysis has applications in forensics, disease research, genetics, and paternity testing by detecting differences in fragment lengths between DNA samples.
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DNA Profiling_HMD_2020.pptx
DNA fingerprinting is a technique used to distinguish between individuals using samples of their DNA. It was invented in 1985 by Alec Jeffreys at the University of Leicester. DNA fingerprinting involves extracting DNA from samples, amplifying specific locations in the DNA called STRs using PCR, then analyzing the STRs using capillary electrophoresis to generate a DNA profile that can be used to identify individuals. Statistical analysis using the product rule allows DNA profiles to be individualized with probabilities of 1 in many billions.
DNA Fingerprinting
This document provides an overview of DNA fingerprinting (also called DNA profiling). It discusses the stages of DNA fingerprinting including extraction, cutting, separation, transfer and analysis. The principle of DNA fingerprinting is that restriction enzymes cut DNA at unique sites, creating variable fragment patterns between individuals. Applications include determining paternity, criminal identification using DNA from crime scenes, and personal identification. Advantages are that DNA profiling can identify individuals with certainty, while limitations include samples being easily contaminated and complex patterns. The document also discusses uses in forensics, plants, centers that perform DNA fingerprinting, and concludes with an overview.
DNA FINGERPRINTING SMG
DNA fingerprinting is a technique used to identify individuals by analyzing genetic variations in DNA sequences found in certain regions of the genome. It was developed in 1985 by Alec Jeffreys and involves isolating DNA from a sample, cutting it with restriction enzymes, and comparing patterns of DNA fragments between individuals using probes that bind to variable number tandem repeats. DNA fingerprinting relies on differences in repetitive sequences that vary between people but are identical in each cell of an individual, allowing a unique genetic profile to be generated for identification purposes in forensic investigations and paternity testing.
DNA fingerprinting
This document summarizes DNA fingerprinting, which involves analyzing variable tandem repeat regions in DNA to generate unique genetic profiles for identification purposes. It describes how DNA is extracted from samples, cut using restriction enzymes, separated via gel electrophoresis, and analyzed using probes to develop a fingerprint. Key applications include identifying suspects in criminal cases, solving paternity disputes, diagnosing genetic disorders, and personal identification. The technique was pioneered in 1984 by Alec Jeffreys and first used in court in 1987. Famous cases that have utilized DNA fingerprinting include identifying the killer in the Colin Pitchfork murder case and establishing Steve Bing's paternity of Elizabeth Hurley's son.
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chapter 9 - dna powerpoint for forensics
This document provides an overview of DNA and its use in forensics. It discusses how DNA can be used to link suspects to crime scenes, identify missing or dead persons, and link suspects to events. It describes the structure of DNA, including the double helix structure and base pairing. It explains how DNA is replicated and how genes instruct the production of proteins. The document outlines early DNA fingerprinting techniques like RFLPs and VNTRs, and more modern techniques using STR analysis and PCR. It discusses the use of DNA fingerprinting in paternity testing, criminal cases, and Y-STR analysis.
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Microarrays (DNA chips) allow large numbers of DNA probes to be immobilized on a solid surface like nylon or glass. They have various applications including identifying genes responsible for development, diseases, and single nucleotide polymorphisms. Restriction fragment length polymorphism (RFLP) detects length variations in DNA fragments after restriction enzyme digestion, revealing polymorphisms. DNA fingerprinting analyzes variable numbers of tandem repeats in satellite DNA, producing unique patterns that can identify individuals in forensic investigations.
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DNA fingerprinting was developed in 1984 by Alec J. Jeffrey at the University of Leicester. It involves analyzing variable number tandem repeats (VNTRs) in DNA to generate a unique profile for identifying individuals. DNA fingerprinting has six main steps: isolating DNA, cutting it with restriction enzymes, transferring fragments to nylon, probing with radioactive markers, and comparing the final fingerprint to other samples. This technique can solve crimes by matching DNA from a crime scene to suspects, determine paternity in inheritance cases, and has been used in famous cases like those involving Elizabeth Hurley and OJ Simpson.
Dna fingerprinting
DNA fingerprinting is a technique that allows identification of an individual from biological samples based on their unique DNA fingerprint. It works by detecting variations in short tandem repeats (STRs) in the DNA, which differ in length between individuals. The process involves extracting DNA from a sample, using restriction enzymes to cut the DNA at specific sites, separating the fragments via gel electrophoresis, and amplifying the STR regions via polymerase chain reaction to facilitate comparison to known DNA profiles. DNA fingerprinting has applications in forensics, paternity testing, and medical diagnosis of inherited disorders, but risks discrimination and privacy issues if not properly regulated and restricted.
Role of dna fingerprinting in crimes
This document discusses the role of DNA fingerprinting in solving crimes. It provides details on the history of DNA fingerprinting, how DNA fingerprints are made in the lab through restriction enzymes and gel electrophoresis, and examples of how DNA profiling has been used to identify criminals, determine paternity and diagnose genetic disorders. The stages of DNA profiling are outlined and it is noted that DNA fingerprinting can provide strong evidence if a suspect's profile matches the biological evidence left at a crime scene.
SNP
This document discusses single nucleotide polymorphisms (SNPs). It defines SNPs as variations in DNA sequences that occur when a single nucleotide differs between members of a species. SNPs are the most common type of genetic variation. The document outlines the characteristics of SNPs, how they are used as genetic markers, and various methods for SNP genotyping, including direct sequencing, TaqMan assays, and microchips. It also discusses the advantages and applications of SNPs in areas like gene discovery, disease risk profiling, and genetic variation studies.
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DNA fingerprinting is a powerful forensic technique that analyzes variations in DNA sequences to identify individuals. It works by cutting DNA into fragments of different lengths using restriction enzymes, then comparing fragment length patterns between samples. While now widely accepted, DNA fingerprinting faced initial resistance when first introduced. This document discusses the history and development of DNA fingerprinting techniques, including early methods like RFLPs and VNTRs, and more recent STR analysis using PCR amplification, which allows analysis from smaller samples and is now the standard forensic approach. Applications of DNA fingerprinting include solving criminal cases, paternity testing, and tracing ancestry.
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DNA fingerprinting is a technique that uses variations in DNA sequences to distinguish individuals. It involves extracting DNA from a sample, amplifying it if needed, cutting it with restriction enzymes to isolate variable number tandem repeats, separating the fragments by size using gel electrophoresis, treating the DNA fragments with a radioactive probe, and exposing X-ray film to create an image-based fingerprint of the sample's DNA pattern. This fingerprint can then be used to identify individuals or determine familial relationships for various applications like criminal investigations, identifying remains, and tracing hereditary conditions.
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DNA fingerprinting is a technique that uses variations in DNA sequences to distinguish individuals. It involves extracting DNA from a sample, amplifying it if needed, cutting it with restriction enzymes to isolate variable number tandem repeats, separating the fragments by size using gel electrophoresis, treating the DNA fragments with a radioactive probe, and exposing X-ray film to create an image-based fingerprint of the sample's DNA pattern. This fingerprint can then be used to identify individuals or determine familial relationships for various applications like criminal investigations, identifying remains, and tracing hereditary conditions.
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DNA Fingerprinting
DNA fingerprinting is a technique used for identification by extracting and analyzing the base pair pattern of an individual's DNA. It involves isolating DNA from a sample, cutting the DNA into fragments using restriction enzymes, and comparing the fragment patterns on a gel to identify individuals. The main applications of DNA fingerprinting are in solving criminal cases by matching DNA evidence to suspects, diagnosing inherited diseases, and determining biological relationships in areas like paternity testing.
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Copy Of Dna Sequencing
RFLP analysis involves fragmenting DNA samples with restriction enzymes, separating the fragments by length through gel electrophoresis, and detecting length polymorphisms between individuals. Any single nucleotide change can create or destroy a restriction site, altering the length of the resulting fragment. RFLP analysis has applications in forensics, disease research, genetics, and paternity testing by detecting differences in fragment lengths between DNA samples.
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DNA is important evidence in forensic analysis. This presentation will help in understanding DNA and Fingerprinting technology
DNA Profiling_HMD_2020.pptx
DNA fingerprinting is a technique used to distinguish between individuals using samples of their DNA. It was invented in 1985 by Alec Jeffreys at the University of Leicester. DNA fingerprinting involves extracting DNA from samples, amplifying specific locations in the DNA called STRs using PCR, then analyzing the STRs using capillary electrophoresis to generate a DNA profile that can be used to identify individuals. Statistical analysis using the product rule allows DNA profiles to be individualized with probabilities of 1 in many billions.
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This document provides an overview of DNA fingerprinting (also called DNA profiling). It discusses the stages of DNA fingerprinting including extraction, cutting, separation, transfer and analysis. The principle of DNA fingerprinting is that restriction enzymes cut DNA at unique sites, creating variable fragment patterns between individuals. Applications include determining paternity, criminal identification using DNA from crime scenes, and personal identification. Advantages are that DNA profiling can identify individuals with certainty, while limitations include samples being easily contaminated and complex patterns. The document also discusses uses in forensics, plants, centers that perform DNA fingerprinting, and concludes with an overview.
DNA FINGERPRINTING SMG
DNA fingerprinting is a technique used to identify individuals by analyzing genetic variations in DNA sequences found in certain regions of the genome. It was developed in 1985 by Alec Jeffreys and involves isolating DNA from a sample, cutting it with restriction enzymes, and comparing patterns of DNA fragments between individuals using probes that bind to variable number tandem repeats. DNA fingerprinting relies on differences in repetitive sequences that vary between people but are identical in each cell of an individual, allowing a unique genetic profile to be generated for identification purposes in forensic investigations and paternity testing.
DNA fingerprinting
This document summarizes DNA fingerprinting, which involves analyzing variable tandem repeat regions in DNA to generate unique genetic profiles for identification purposes. It describes how DNA is extracted from samples, cut using restriction enzymes, separated via gel electrophoresis, and analyzed using probes to develop a fingerprint. Key applications include identifying suspects in criminal cases, solving paternity disputes, diagnosing genetic disorders, and personal identification. The technique was pioneered in 1984 by Alec Jeffreys and first used in court in 1987. Famous cases that have utilized DNA fingerprinting include identifying the killer in the Colin Pitchfork murder case and establishing Steve Bing's paternity of Elizabeth Hurley's son.
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This document discusses several molecular techniques used in microbiology, including restriction fragment length polymorphism (RFLP), pulsed-field gel electrophoresis (PFGE), and cleaved amplified polymorphic sequences (CAPS). RFLP detects genetic variations by cutting DNA with restriction enzymes and comparing fragment lengths. PFGE improves on standard gel electrophoresis by applying an alternating electric field to better separate very large DNA molecules. CAPS combines PCR and RFLP to detect single base changes without radioactive probes. These techniques are useful for genome mapping, disease analysis, and forensic or epidemiological investigations.
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Dna chips, RFLPs & dna fingerprint
Microarrays (DNA chips) allow large numbers of DNA probes to be immobilized on a solid surface like nylon or glass. They have various applications including identifying genes responsible for development, diseases, and single nucleotide polymorphisms. Restriction fragment length polymorphism (RFLP) detects length variations in DNA fragments after restriction enzyme digestion, revealing polymorphisms. DNA fingerprinting analyzes variable numbers of tandem repeats in satellite DNA, producing unique patterns that can identify individuals in forensic investigations.
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DNA fingerprinting was developed in 1984 by Alec J. Jeffrey at the University of Leicester. It involves analyzing variable number tandem repeats (VNTRs) in DNA to generate a unique profile for identifying individuals. DNA fingerprinting has six main steps: isolating DNA, cutting it with restriction enzymes, transferring fragments to nylon, probing with radioactive markers, and comparing the final fingerprint to other samples. This technique can solve crimes by matching DNA from a crime scene to suspects, determine paternity in inheritance cases, and has been used in famous cases like those involving Elizabeth Hurley and OJ Simpson.
Dna fingerprinting
DNA fingerprinting is a technique that allows identification of an individual from biological samples based on their unique DNA fingerprint. It works by detecting variations in short tandem repeats (STRs) in the DNA, which differ in length between individuals. The process involves extracting DNA from a sample, using restriction enzymes to cut the DNA at specific sites, separating the fragments via gel electrophoresis, and amplifying the STR regions via polymerase chain reaction to facilitate comparison to known DNA profiles. DNA fingerprinting has applications in forensics, paternity testing, and medical diagnosis of inherited disorders, but risks discrimination and privacy issues if not properly regulated and restricted.
Role of dna fingerprinting in crimes
This document discusses the role of DNA fingerprinting in solving crimes. It provides details on the history of DNA fingerprinting, how DNA fingerprints are made in the lab through restriction enzymes and gel electrophoresis, and examples of how DNA profiling has been used to identify criminals, determine paternity and diagnose genetic disorders. The stages of DNA profiling are outlined and it is noted that DNA fingerprinting can provide strong evidence if a suspect's profile matches the biological evidence left at a crime scene.
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This document discusses single nucleotide polymorphisms (SNPs). It defines SNPs as variations in DNA sequences that occur when a single nucleotide differs between members of a species. SNPs are the most common type of genetic variation. The document outlines the characteristics of SNPs, how they are used as genetic markers, and various methods for SNP genotyping, including direct sequencing, TaqMan assays, and microchips. It also discusses the advantages and applications of SNPs in areas like gene discovery, disease risk profiling, and genetic variation studies.
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Denis is a dynamic and results-driven Chief Information Officer (CIO) with a distinguished career spanning information systems analysis and technical project management. With a proven track record of spearheading the design and delivery of cutting-edge Information Management solutions, he has consistently elevated business operations, streamlined reporting functions, and maximized process efficiency.
Certified as an ISO/IEC 27001: Information Security Management Systems (ISMS) Lead Implementer, Data Protection Officer, and Cyber Risks Analyst, Denis brings a heightened focus on data security, privacy, and cyber resilience to every endeavor.
His expertise extends across a diverse spectrum of reporting, database, and web development applications, underpinned by an exceptional grasp of data storage and virtualization technologies. His proficiency in application testing, database administration, and data cleansing ensures seamless execution of complex projects.
What sets Denis apart is his comprehensive understanding of Business and Systems Analysis technologies, honed through involvement in all phases of the Software Development Lifecycle (SDLC). From meticulous requirements gathering to precise analysis, innovative design, rigorous development, thorough testing, and successful implementation, he has consistently delivered exceptional results.
Throughout his career, he has taken on multifaceted roles, from leading technical project management teams to owning solutions that drive operational excellence. His conscientious and proactive approach is unwavering, whether he is working independently or collaboratively within a team. His ability to connect with colleagues on a personal level underscores his commitment to fostering a harmonious and productive workplace environment.
Date: May 29, 2024
Tags: Information Security, ISO/IEC 27001, ISO/IEC 42001, Artificial Intelligence, GDPR
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Walmart Business+ and Spark Good for Nonprofits.pdf
"Learn about all the ways Walmart supports nonprofit organizations.
You will hear from Liz Willett, the Head of Nonprofits, and hear about what Walmart is doing to help nonprofits, including Walmart Business and Spark Good. Walmart Business+ is a new offer for nonprofits that offers discounts and also streamlines nonprofits order and expense tracking, saving time and money.
The webinar may also give some examples on how nonprofits can best leverage Walmart Business+.
The event will cover the following::
Walmart Business + (https://business.walmart.com/plus) is a new shopping experience for nonprofits, schools, and local business customers that connects an exclusive online shopping experience to stores. Benefits include free delivery and shipping, a 'Spend Analytics” feature, special discounts, deals and tax-exempt shopping.
Special TechSoup offer for a free 180 days membership, and up to $150 in discounts on eligible orders.
Spark Good (walmart.com/sparkgood) is a charitable platform that enables nonprofits to receive donations directly from customers and associates.
Answers about how you can do more with Walmart!"
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What is Digital Literacy? A guest blog from Andy McLaughlin, University of Ab...
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ISO/IEC 27001, ISO/IEC 42001, and GDPR: Best Practices for Implementation and...
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Walmart Business+ and Spark Good for Nonprofits.pdf
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How to Create a More Engaging and Human Online Learning Experience
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DNA Fingerprinting.ppt
- 2. Why Use DNA Fingerprinting? DNA fingerprinting is a way of telling individuals of the same species apart DNA sequences are variable and can therefore be used as identifying characteristics. DNA fingerprinting has advantages over other sources of evidence (fingerprints, blood type, etc.): – Highly accurate. – Can be gathered from trace crime scene evidence.
- 3. How do you take a DNA fingerprint? One way: Restriction Fragment Length Polymorphisms (huh?) aka RFLP Restriction enzymes are molecules that can cut DNA into pieces --> each enzyme cuts at a very specific DNA sequence While all human beings share roughly the same DNA sequence, there are in fact a small number of differences these differences can be seen by restriction enzymes
- 5. Individual 1 A B C D E DNA
- 6. Individual 2 A B C D
- 7. Individual 1 vs. Individual 2 A B C D Individual 2 A B C D E Individual 1
- 8. Summary Essentially, once the DNA has been cut by the enzymes, we will have DNA fragments of various sizes Each individual’s banding patterns should be different because the restriction enzymes will cut each person’s DNA at different points Fragments of different sizes will travel different distances along a gel when an electric current is passed through it
- 9. Agarose Gel Electrophoresis Different banding patterns from different individuals