This document discusses the theory, instrumentation, and applications of dispersive and Fourier transform infrared (FTIR) spectroscopy. It begins with an introduction to IR spectroscopy and the IR region. It then covers dispersive IR instrumentation, which uses prism or grating monochromators to separate wavelengths, and has limitations like slow scan speeds and limited resolution. The document introduces FTIR instrumentation, which uses an interferometer to simultaneously measure all wavelengths and overcomes the limitations of dispersive IR. It concludes that FTIR provides faster, more accurate and sensitive analysis compared to dispersive IR.
IR SPECTROSCOPY, INTRODUCTION, PRINCIPLE, THEORY, FATE OF ABSORBED RADIATION, FERMI RESONANCE, FINGERPRINT REGION, VIBRATIONS, FACTORS AFFECTING ABSORPTION OF IR RADIATION, SAMPLING TECHNIQUES, APPLICATIONS OF IR SPECTROSCOPY.
Detectors are the brain of any chromatograhic system. It help us to record the chromatogram based on certain characteristics of the analyte and help us in identifying that compound both qualitatively and quantitatively.
Quadrupole and Time of Flight Mass analysers.Gagangowda58
Description about important mass analysers Quadrupole and TOF: Principle, Construction and Working, Advantages and Disadvantages and their Applications.
Introduction
working principle
fragmentation process
general rules for fragmentation
general modes of fragmentation
metastable ions
isotopic peaks
applications
In this slide contains principle of IR spectroscopy and sampling techniques.
Presented by: R.Banuteja (Department of pharmaceutical analysis).
RIPER, anantpur.
An Infrared spectrum represents a fingerprint of a sample with absorption peaks which correspond to the frequencies of vibrations between the bonds of the atoms making up the material-Because each different material is a unique combination of atoms, no two compounds produce the exact same spectrum, therefore IR can result in a unique identification of every different kind of material!
IR SPECTROSCOPY, INTRODUCTION, PRINCIPLE, THEORY, FATE OF ABSORBED RADIATION, FERMI RESONANCE, FINGERPRINT REGION, VIBRATIONS, FACTORS AFFECTING ABSORPTION OF IR RADIATION, SAMPLING TECHNIQUES, APPLICATIONS OF IR SPECTROSCOPY.
Detectors are the brain of any chromatograhic system. It help us to record the chromatogram based on certain characteristics of the analyte and help us in identifying that compound both qualitatively and quantitatively.
Quadrupole and Time of Flight Mass analysers.Gagangowda58
Description about important mass analysers Quadrupole and TOF: Principle, Construction and Working, Advantages and Disadvantages and their Applications.
Introduction
working principle
fragmentation process
general rules for fragmentation
general modes of fragmentation
metastable ions
isotopic peaks
applications
In this slide contains principle of IR spectroscopy and sampling techniques.
Presented by: R.Banuteja (Department of pharmaceutical analysis).
RIPER, anantpur.
An Infrared spectrum represents a fingerprint of a sample with absorption peaks which correspond to the frequencies of vibrations between the bonds of the atoms making up the material-Because each different material is a unique combination of atoms, no two compounds produce the exact same spectrum, therefore IR can result in a unique identification of every different kind of material!
As environmental regulations become more stringent and as more gaseous pollutants need to be monitored, there is a need for a cost-effective measurement technique to evaluate emissions from electric generating units and other processing facilities. A Fourier Transform Infrared (FTIR) analyzer is a solution for this issue. FTIR analysis is a technology that generates real-time emissions data at high sensitivities. FTIR analyzers can measure nearly all gaseous compounds while requiring little to no calibration. The two biggest advantages that FTIR analysis has over traditional measurement methodologies are its ability to measure many different gaseous species simultaneously and its ability to generate immediate results.
A method of obtaining an Infrared spectrum by measuring the interferogram of a sample using an interferometer, then performing a Fourier Transform upon the interferogram to obtain the spectrum.
FTIR SPECTROSCOPY,
Principle, Theory, Instrumentation and Application in Pharmaceutical Industry
IR Spectroscopy- Absorption Theory
Type of Vibrations & Vibration Energy level
FTIR Spectrophotometer-Instrumentation
Operation of the Spectrophotometer
Qualification & Calibration
IR Absorption by Organic compounds
Application
FDA citation in FTIR Analysis-Pharmaceutical Industries
Fourier transform infrared spectroscopy (FTIR) is a largely used technique to identify the functional groups in the materials (gas, liquid, and solid) by using the beam of infrared radiations. An infrared spectroscopy measures the absorption of IR radiation made by each bond in the molecule and as a result gives spectrum which is commonly designated as % transmittance versus wavenumber (cm−1). The IR region is at lower energy and higher wavelength than the UV-visible light and has higher energy or shorter wavelength than the microwave radiations. For the determination of functional groups in a molecule, it must be IR active. An IR active molecule is the one which has dipole moment. When the IR radiation interacts with the covalent bond of the materials having an electric dipole, the molecule absorbs energy, and the bond starts back and forth oscillation. Therefore, the oscillation which cause the change in the net dipole moment of the molecule should absorb IR radiations.
A single atom doesn’t absorb IR radiation as it has no chemical bond.
Symmetrical molecules also do not absorbed IR radiation, because of zero dipole moment. For example, H2 molecule has two H atoms; both cancel the effect of each other and giving zero dipole moment to H2 molecule. Therefore, H2 molecule is not an IR active molecule. On the other hand, HF is an IR active molecule, because when IR radiation interacts with HF molecule, the charge transferred toward the fluorine atom and as a result fluorine becomes partial negative and hydrogen becomes partial positive, giving net dipole moment to H-F molecule. A particular IR radiation will be absorbed by a particular bond in the molecule, because every bond has their particular natural vibrational frequency. For example, a molecule such as acetic acid (CH3COOH) containing various bonds (C-C, C-H, C-O, O-H, and C=O), all these bonds are absorbed at specific wavelength and are not affected by other bond. In general we can say that two molecules with different structures don’t have the same infrared spectrum, although some of the frequencies might be same.
Introduction to Fourier Transfer Infrared SpectroscopyRahulVerma550005
The preferred method of infrared spectroscopy is known as Fourier Transform InfraRed (FT-IR). Infrared spectroscopy involves passing IR photons through a sample. The sample absorbs some of the infrared light and passes some of it through (transmitted). The resulting spectrum depicts the sample's molecule absorption and transmission, resulting in a molecular fingerprint.
FTIR is a characterisation technique to evaluate the composition of the sample such as nanoparticles, nanospheres, chemical drugs and composites etc. It is time efficient as useful for bulk sample analysis.
Infrared Spectroscopy-Principles, Instrumentation and Applicationsuriyachem27
In this presentation, I mentioned Principles, Vibration, Potential energy diagram , Harmonic Oscillator, Vibration mode, instrumentation and application of IR spectroscopy . I referred Principles of Instrumental analysis, Seventh edition, Skoog, Holler and crouch. Once you read this Presentation you will get clear point about IR spectroscopy.
Introduction
Instrumentation
Sampling techniques
Group frequencies
Factors affecting group frequencies
Complementarity of IR and Raman spectroscopy
Applications of Infrared spectroscopy
This is a presentation by Dada Robert in a Your Skill Boost masterclass organised by the Excellence Foundation for South Sudan (EFSS) on Saturday, the 25th and Sunday, the 26th of May 2024.
He discussed the concept of quality improvement, emphasizing its applicability to various aspects of life, including personal, project, and program improvements. He defined quality as doing the right thing at the right time in the right way to achieve the best possible results and discussed the concept of the "gap" between what we know and what we do, and how this gap represents the areas we need to improve. He explained the scientific approach to quality improvement, which involves systematic performance analysis, testing and learning, and implementing change ideas. He also highlighted the importance of client focus and a team approach to quality improvement.
The Indian economy is classified into different sectors to simplify the analysis and understanding of economic activities. For Class 10, it's essential to grasp the sectors of the Indian economy, understand their characteristics, and recognize their importance. This guide will provide detailed notes on the Sectors of the Indian Economy Class 10, using specific long-tail keywords to enhance comprehension.
For more information, visit-www.vavaclasses.com
Read| The latest issue of The Challenger is here! We are thrilled to announce that our school paper has qualified for the NATIONAL SCHOOLS PRESS CONFERENCE (NSPC) 2024. Thank you for your unwavering support and trust. Dive into the stories that made us stand out!
Model Attribute Check Company Auto PropertyCeline George
In Odoo, the multi-company feature allows you to manage multiple companies within a single Odoo database instance. Each company can have its own configurations while still sharing common resources such as products, customers, and suppliers.
The Art Pastor's Guide to Sabbath | Steve ThomasonSteve Thomason
What is the purpose of the Sabbath Law in the Torah. It is interesting to compare how the context of the law shifts from Exodus to Deuteronomy. Who gets to rest, and why?
2024.06.01 Introducing a competency framework for languag learning materials ...Sandy Millin
http://sandymillin.wordpress.com/iateflwebinar2024
Published classroom materials form the basis of syllabuses, drive teacher professional development, and have a potentially huge influence on learners, teachers and education systems. All teachers also create their own materials, whether a few sentences on a blackboard, a highly-structured fully-realised online course, or anything in between. Despite this, the knowledge and skills needed to create effective language learning materials are rarely part of teacher training, and are mostly learnt by trial and error.
Knowledge and skills frameworks, generally called competency frameworks, for ELT teachers, trainers and managers have existed for a few years now. However, until I created one for my MA dissertation, there wasn’t one drawing together what we need to know and do to be able to effectively produce language learning materials.
This webinar will introduce you to my framework, highlighting the key competencies I identified from my research. It will also show how anybody involved in language teaching (any language, not just English!), teacher training, managing schools or developing language learning materials can benefit from using the framework.
Operation “Blue Star” is the only event in the history of Independent India where the state went into war with its own people. Even after about 40 years it is not clear if it was culmination of states anger over people of the region, a political game of power or start of dictatorial chapter in the democratic setup.
The people of Punjab felt alienated from main stream due to denial of their just demands during a long democratic struggle since independence. As it happen all over the word, it led to militant struggle with great loss of lives of military, police and civilian personnel. Killing of Indira Gandhi and massacre of innocent Sikhs in Delhi and other India cities was also associated with this movement.
We all have good and bad thoughts from time to time and situation to situation. We are bombarded daily with spiraling thoughts(both negative and positive) creating all-consuming feel , making us difficult to manage with associated suffering. Good thoughts are like our Mob Signal (Positive thought) amidst noise(negative thought) in the atmosphere. Negative thoughts like noise outweigh positive thoughts. These thoughts often create unwanted confusion, trouble, stress and frustration in our mind as well as chaos in our physical world. Negative thoughts are also known as “distorted thinking”.
How to Make a Field invisible in Odoo 17Celine George
It is possible to hide or invisible some fields in odoo. Commonly using “invisible” attribute in the field definition to invisible the fields. This slide will show how to make a field invisible in odoo 17.
3. INTRODUCTION
Spectroscopy is the branch of science dealing with
the study of interaction of electromagnetic radiation with
matter.
IR spectroscopy is Absorption spectroscopy in
which molecular vibrations observed due to absorption of IR
radiation.
Infrared radiation was discovered in 1800
by William Herschel.
3
4. The range of EMR between the visible and microwaves
region is called INFRARED region(14000-40 cm-1 ).
4
5. IR REGION
From application and instrumentation point of view
infrared region is subdivided into
Wave length (m)
Wave number(cm-1 )
Near infrared
0.75-2.5
14000-4000
Mid infrared
2.5-50
4000-400
Far infrared
50-300
400-40
Region
Mid IR
(4000-40 cm-1)
Functional group
region
Finger Print/Single
bond region
(4000-1400 cm-1)
(1400-40cm-1)
Stretching vibrations occurs in
F.G. region
Bending vibrations occurs in F.P.
region
5
6. General Purpose Of
IR Radiation
Infrared light is used in industrial, scientific, and
medical applications.
• In Telescopes to detect planets
• Finding heat leaks from houses
6
7. Contd...
• Infrared thermal-imaging cameras are used to detect heat loss
in insulated systems, to observe changing blood flow in the
skin, and to detect overheating of electrical apparatus.
• Night-vision devices
• Remote temperature sensing, short-ranged wireless
communication, spectroscopy, and weather forecasting.
7
8. Pharmaceutical Applications
Qualitative determination of substances
Structural elucidation by determining the functional groups.
Detection of impurities
Identification of geometrical isomers for both organic &
inorganic samples
Detection of presence of water in sample
Quantitative determination of sample by using Beer’s-Lamberts
law
8
9. PRINCIPLE
In any molecule , atoms or groups of atoms are connected by
bonds which are similar to springs and not rigid in nature.
Bonded atoms having different strengths due to different
masses.
Absorption of IR energy will cause vibrational changes in
molecule and a peak will be observed, when
9
10. Contd…
Conditions to obtain IR spectrum
1) Selection rule for IR spectroscopy: Dipole moment of bonds
should change during vibration.
2) When Applied infrared frequency = Natural frequency of
vibration
3) When covalent bonds are polar in nature
When sample obeys these conditions, then it gets vibrated
by absorbing radiation and gives IR spectrum.
10
11. Hooke’s law
Hooke's law
of elasticity is an
approximation
which states that the
extension of a spring
is in direct
proportion with the
load added to it as
long as this load
does not exceed the
elastic limit.
K = force constant
(in dynes/cm)
m = atomic masses of atom 1 & 2
Used to calculate approximate position of band
11
12. TYPES OF VIBRATIONS
Stretching Mode
In plane bending vibrations
Scissoring
Rocking
Out plane bending vibrations
Symmetric
Asymmetric
Wagging
Twisting
12
13. Radiation sources
They must produce intense & steady radiation.
Nernst Glower
heated rare earth oxide rod
1-50 µm
(zirconium, yttria, thoria) (~1500 (mid- to far-IR)
K)
Globar
heated Silicon Carbide rod
(~1500 K)
1-50 µm
(mid- to far-IR)
W filament lamp
1100 K
0.78-2.5 µm
(Near-IR)
Hg arc lamp
Hg plasma
50 - 300 µm
(far-IR)
13
14. Monochromators
Filtration of desired frequency of radiation
Monochromators are 2 types
1) Prismatic Monochromator
2) Grating Monochromator
Prismatic Monochromators:
Composed of glass or quartz and coated by alkyl halides (NaCl)
These are 2 types
Mono pass Prismatic Monochromator: radiation will pass once
through the prism
Double pass Prismatic Monochromator: radiation will pass
twice through the prism
14
15. Grating monochromators introduced in 1950’s
High dispersion and resolution than prismatic
monochromators
These are 2 types
Reflection Grating Monochromator are common
than Transmittance Gratings
Gratings are linear grooves or lines which are
made up of Aluminium.
15
16. Detectors/Transducers
Thermocouple
Thermoelectric effect dissimilar metal junction
cheap, slow,
insensitive
Bolometer
Ni, Pt resistance
thermometer (thermistor)
Highly sensitive
<400 cm-1
Golay cell
Metal cylinder with Xe gas
Faster than others &
having wide
wavelength range
Pyro electric
Tri glycine sulfate
piezoelectric material
fast and sensitive
(mid IR)
Photoconductive PbS, CdS, Pb Se light sensitive fast and sensitive
(non-thermal)
cells
(near IR)
16
17. TYPES OF IR
INSTRUMENTS
NonDispersive
Dispersive
FTIR
systems
• Filters used for wavelength selection
& having sample specific Detector
• Sequential scanning of each wave
number takes place
• Widely applied and quite popular in
the far-IR and mid-IR spectrometry.
17
18. Dispersive IR
Instrument
Dispersive IR
instruments are introduced in
1940’s.
Double-beam
instruments are mostly used
than Single beam instrument.
In dispersive IR
sequential scanning of wave
numbers of light takes place.
18
19. In double beam spectrometer , beam separates into two
and passes to sample & reference.
Prismatic monochromators have been replaced with
Grating monochromator.
Dispersive IR failed due to monochromator containing
narrow slits which limit the wave number of radiation.
19
20. (X) It containing all movable parts which causes mechanical
slippage
(X) Slow scan speed
(X) Less resolution, accuracy and sensitivity
(X) Only narrow frequency range can be studied
(X) Involvement of stray light
(X) Atmospheric absorptions by CO,
water also takes place.
To overcome all these problems FTIR has been developed
20
21. Fourier Transform
IR Instrument
FTIR collects all wavelengths simultaneously and scans at
once.
FTIR works based on Michelson Interferometer which
having
• Beam splitter
• Fixed mirror
• Movable mirror
21
23. When the beams are combined an
interference pattern is created
Combined beam reaches detector by
passing through sample
Obtained spectrum is referred as
Interferogram
This will be amplified and translated
into IR spectrum by FTIR
23
24. Advantages
Fast & sensitive
All frequencies can be modulated at once
Simple mechanical design with only one
moving part
No stray light is involved
When using He-Ne laser as internal
standard, no need of external calibration
Availability of easy sampling accessories
Air pollutants like CO, ethylene oxide
etc. can be analysed
24
25. FTIR having significant advantages over
Dispersive IR due to its fast and accurate analysis.
25
26. References
Instrumental Methods Of Chemical Analysis; By Gurudeep
R. Chatwal, pg No. 2.29-2.82
Infra Red Spectroscopy: Fundamentals And Applications;
By Barbara Stuart, pg No. 16-23
Introduction to Spectroscopy, 4th edition, By Pavia,
Lampman, Kriz.
Elementary organic chemistry, By Y.R.Sharma; 2007,Pg
No.69-137
26