The document describes the process of bacterial genome replication in B. stevensonii, emphasizing theta replication and the role of plasmids in genetic transfer through conjugation. It explains how the bacterial cell grows, replicates its chromosomes, and divides to form two identical daughter cells, utilizing structures like pili for plasmid transfer. This mechanism allows for the exchange of genetic material, enhancing bacterial diversity and adaptation.

1. Describe: bacterial genome b.stev

2. REPLICATES before cell division: two daughter cells attached > interior of cell membrane membrane elongates, chromosomes move apart cell doubles in size: chromosomes separate the cell THEN “ pinches” inwards... a new cell wall forms that separates the two new cells and their chromosomal replicas most of the hereditary material: single long circular molecule, DNA - a variety of proteins associated this molecule is the cell’s chromosome (Barnes S. N, Curtis H, 1989)

3. cell wall chromosome cell membrane theta replication growth of cell NEXT REPLICATION

4. new cell wall forms REPLICATED bacterial cells (two identical daughter cells)

5. This type of replication is known as: THETA REPLICATION a bi-directional motion DNA helix unwinds/ replicates / rewinds

6. cell F- cell F+ bacterial chromosome F plasmid bacteria join: pili transfer of F plasmid cell F+ transfer provides: COMPLEMENTARY DNA CONJUGATION

7. PLASMIDS are small pockets of DNA that occur in bacteria carry genetic material for a specific purpose plasmids often conjugate to another bacterium THIS: provides the bacterium with complementary DNA KNOWN AS,” rolling circle replication ” plasmid moves to bacterium via the use of an extension made by host [ carrier ] cell: - this extension is called: pili (Barnes S. N, Curtis H, 1989)

8. Bibliography Barnes S. N, Curtis H. (1989). Biology (5th ed.) . New York: Worth Wikipedia. (2008). Bacterial conjugation – wikipedia, the free encyclopedia . Retrieved October 23, 2008, from en.wikipedia.org/wiki/Bacterial _ conjugation - 41k - The basics – replication . (n.d). Retrieved October 23, 2008, from http//:www. socrates.acadiau.ca/courses/biol/microbiology/ Replication .htm - 9k -