Dna topology

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DNA topology refers to the coiling and knotting of DNA strands. DNA exists in vivo in a negatively supercoiled state, with the strands intertwined about each other. Enzymes called topoisomerases regulate and maintain the appropriate level of supercoiling by introducing transient breaks into DNA strands to allow strand passage and control overwinding or underwinding of the double helix.

Science

Creation of a cDNA library starts with mRNA instead of DNA. Messenger RNA carries encoded information from DNA to ribosomes for translation into protein. To create a cDNA library, these mRNA molecules are treated with the enzyme reverse transcriptase, which is used to make a DNA copy of an mRNA (i.e., cDNA). A cDNA library represents a sampling of the transcribed genes, but a genomic library includes untranscribed regions.

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Dna topology

1. DNA TOPOLOGY

Dna topology

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