How 3 germ layers are formed in Chick that are endoderm, mesoderm and ectoderm.As Chick are polylecithal so cell movements are somewhat restricted and gastrulation is modified as compared to frog.
How 3 germ layers are formed in Chick that are endoderm, mesoderm and ectoderm.As Chick are polylecithal so cell movements are somewhat restricted and gastrulation is modified as compared to frog.
Vittelogenesis is a word developed from Latin vitellus-yolk, and genero-produce
Vitellogenesis (also known as yolk deposition) is the process of yolk formation via nutrients being deposited in the oocyte, or female germ cell involved in reproduction of lecithotrophic organisms. In insects, it starts when the fat body stimulates the release of juvenile hormones and produces vitellogenin protein.
Yolks is the most usual form of food storage in the egg.
Yolks appear in the oocyte in the secondary period of their growth called vittelogenesis.
Thus,the formation and deposition of yolks is known as vittelogenesis
Characteristic
Yolks is a complex variable assembled component.
The principle component are protein,phospholipid and fats in different combination.
Depending upon these component yolks is distinguished into protein yolks and fatty acid
For eg- the avian contain 48.19% water , 16.6 % protein, 32.6% phospholipids and fats and 1% carbohydrates.
Vittelogenesis is a word developed from Latin vitellus-yolk, and genero-produce
Vitellogenesis (also known as yolk deposition) is the process of yolk formation via nutrients being deposited in the oocyte, or female germ cell involved in reproduction of lecithotrophic organisms. In insects, it starts when the fat body stimulates the release of juvenile hormones and produces vitellogenin protein.
Yolks is the most usual form of food storage in the egg.
Yolks appear in the oocyte in the secondary period of their growth called vittelogenesis.
Thus,the formation and deposition of yolks is known as vittelogenesis
Characteristic
Yolks is a complex variable assembled component.
The principle component are protein,phospholipid and fats in different combination.
Depending upon these component yolks is distinguished into protein yolks and fatty acid
For eg- the avian contain 48.19% water , 16.6 % protein, 32.6% phospholipids and fats and 1% carbohydrates.
Chap 5 Cleavage. it's types and patternsSaadHumayun7
Cell division during the early stages of the embryo’s development after fertilisation is referred to as cleavage in embryology. Zygotes of several species possess rapid cell cycle progression without considerable overall growth, resulting in a group of cells of identical size as the initial zygote. The diverse cells produced by cleavage are known as blastomeres, and they group together to form a solid mass known as the morula. The development of the blastula, or the blastocyst in animals, indicates the termination of cleavage.
The mitotic division begins as the zygote travels through the isthmus of the oviduct, termed cleavage, towards the uterus and produces 2, 4, 8, and 16 daughter cells (blastomeres). A morula is an embryo that has 8 to 16 blastomeres. As it progresses into the uterus, the morula continues dividing and develops into a blastocyst.
The transformation from fertilisation to cleavage results from the activation of a mitosis-promoting factor (MPF).Cleavage of Zygote
Human zygote cleavage begins inside the fallopian tube. It is holoblastic, dividing the zygote fully into blastomeres or daughter cells.
After fertilisation, the first cleavage occurs about 24 to 30 hours later. It creates two blastomeres by longitudinally dividing the zygote (one mildly larger than the other).
The second cleavage takes place forty hours later.
After fertilisation, there is a third cleavage approximately 72 hours later. During these early cleavages, the young embryo progresses down the fallopian tube towards the uterus.
The embryo enters the uterus at the end of the fourth day. It is referred to as morula and resembles a mulberry. There are 32 cells in this solid morula. The cleavage is radial and of an indeterminate kind in human zygotes.
Cell Cleavage Mechanism
The zygote begins cleaving once fertilisation occurs, and a new organism starts to develop. Cleavage furrow refers to the area where cleavage begins.Two coordinated mechanisms combine to produce cleavage.
Karyokinesis, or the division of the nucleus during mitosis, is the first of these cyclic mechanisms. The mechanical force behind this division is the mitotic spindle, which has microtubules made of tubulin (a protein that comprises the sperm flagellum).
Cytokinesis, or cell division, is the second phase. An actin-based contractile ring of microfilaments serves as the mechanical force behind cytokinesis.
The initiation of zygotic transcription and the termination of cleavage coincides. This transitional stage in non-mammals is known as the mid-blastula transition and is regulated by the nuclear-to-cytoplasmic ratio.
Types of Cleavage
During the cleavage period, there is a significant degree of reorganisation, and the cytoplasmic contents primarily determine the types of cleavage.
Determinate Cleavage
Determinate cleavage, also known as mosaic cleavage, is a type of cleavage based on the potency of blastomeres where each blastomere has a predetermined developmental fate and is not qualita
All living beings are made up of cells. The structural and functional unit of life is a cell which is the building block of the body. New cell arises from the pre-existing cells by the process of cell division.
Cell division occurs in all living organisms. In unicellular organisms, cell division directly produces two individuals. In multicellular organisms or higher-level organisms, life begins from a single cell, as a zygote, whIch divides and redivides mitotically into a number of cells to form a complete organism.
In multicellular organisms, there are two types of cells.
a)The somatic cells or the body cells- They form the body of an organism.
b)The reproductive cells or sex cells- They are gamete-producing cells.
Richard's entangled aventures in wonderlandRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
Multi-source connectivity as the driver of solar wind variability in the heli...Sérgio Sacani
The ambient solar wind that flls the heliosphere originates from multiple
sources in the solar corona and is highly structured. It is often described
as high-speed, relatively homogeneous, plasma streams from coronal
holes and slow-speed, highly variable, streams whose source regions are
under debate. A key goal of ESA/NASA’s Solar Orbiter mission is to identify
solar wind sources and understand what drives the complexity seen in the
heliosphere. By combining magnetic feld modelling and spectroscopic
techniques with high-resolution observations and measurements, we show
that the solar wind variability detected in situ by Solar Orbiter in March
2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
to one active region (12961) and then across to another region (12957). This
is refected in the in situ measurements, which show the transition from fast
to highly Alfvénic then to slow solar wind that is disrupted by the arrival of
a coronal mass ejection. Our results describe solar wind variability at 0.5 au
but are applicable to near-Earth observatories.
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
Cancer cell metabolism: special Reference to Lactate PathwayAADYARAJPANDEY1
Normal Cell Metabolism:
Cellular respiration describes the series of steps that cells use to break down sugar and other chemicals to get the energy we need to function.
Energy is stored in the bonds of glucose and when glucose is broken down, much of that energy is released.
Cell utilize energy in the form of ATP.
The first step of respiration is called glycolysis. In a series of steps, glycolysis breaks glucose into two smaller molecules - a chemical called pyruvate. A small amount of ATP is formed during this process.
Most healthy cells continue the breakdown in a second process, called the Kreb's cycle. The Kreb's cycle allows cells to “burn” the pyruvates made in glycolysis to get more ATP.
The last step in the breakdown of glucose is called oxidative phosphorylation (Ox-Phos).
It takes place in specialized cell structures called mitochondria. This process produces a large amount of ATP. Importantly, cells need oxygen to complete oxidative phosphorylation.
If a cell completes only glycolysis, only 2 molecules of ATP are made per glucose. However, if the cell completes the entire respiration process (glycolysis - Kreb's - oxidative phosphorylation), about 36 molecules of ATP are created, giving it much more energy to use.
IN CANCER CELL:
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
introduction to WARBERG PHENOMENA:
WARBURG EFFECT Usually, cancer cells are highly glycolytic (glucose addiction) and take up more glucose than do normal cells from outside.
Otto Heinrich Warburg (; 8 October 1883 – 1 August 1970) In 1931 was awarded the Nobel Prize in Physiology for his "discovery of the nature and mode of action of the respiratory enzyme.
WARNBURG EFFECT : cancer cells under aerobic (well-oxygenated) conditions to metabolize glucose to lactate (aerobic glycolysis) is known as the Warburg effect. Warburg made the observation that tumor slices consume glucose and secrete lactate at a higher rate than normal tissues.
This presentation explores a brief idea about the structural and functional attributes of nucleotides, the structure and function of genetic materials along with the impact of UV rays and pH upon them.
Comparing Evolved Extractive Text Summary Scores of Bidirectional Encoder Rep...University of Maribor
Slides from:
11th International Conference on Electrical, Electronics and Computer Engineering (IcETRAN), Niš, 3-6 June 2024
Track: Artificial Intelligence
https://www.etran.rs/2024/en/home-english/
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
2. Cleavage stage and and Fate Maps
Cleavage is the first phase of embryonic development by mitotic cell
division.
Introduction
Levels of Cleavage types:
Full splinting or cleavage.
Partial splinting or cleavage.
Surperfacial cleavage or cleavage.
Cleavage stages:
2 cell stage 4 cell stage 8 cell stage 16 cell stage 32 cell
stage Morula Blastula.
Fate maps:
In amphibians
In birds
In Mammals
3. Diagram explaining the development of embryos within the
Fallopian Tubes (cleavage stages)
5. • After fertilization and formation of the zygote(the fertilized ova) begins the first
stages of early embryonic formation, which consists of:
A series of indirect divisions (mitotic) known as the phase of cleavage or
(segmentation) where the fertilized egg is divided into several successive divisions
produced Blastomeres.
• At the begging of cleavage the blasts are initially large and then become
smaller with the progress in the process of splinting or cleavage to form blastula
at the end of the cleavage phases.
Cleavage Introduction
6. before the fertilized egg begins the process of splinting, it prepares internally by
increasing the following:
1. Increasing the composition of protein.
2. Multiplies Genetic material or DNA chromosomes.
3. Formation of the fertilization membrane.
4. Formation of the hyaline layer (protection or preservation of the blastomeres
together).
Cleavage Introduction
7. What is cleavage?
Cleavage is a rapid series of mitotic divisions that occur just after fertilization.
There are two critical reasons why cleavage is so important:
1. Generation of a large number of cells:
That can undergo differentiation and gastrulation to form organs.
2. Increase in the nucleus / cytoplasmic ratio:
Eggs need a lot of cytoplasm to support embryogenesis. It is difficult or
impossible for one nucleus to support a huge cytoplasm, and oocytes are one
of the largest cells that exist. One small nucleus just cannot transcribe
enough RNA to meet the needs of the huge cytoplasm.
A larger nucleus to cytoplasmic ratio is optimal for cell function. Cell
division occurs rapidly after fertilization to correct this problem.
8. 1. During the process of cleavage ,the cell cycle consists of only 2 phases:
The (S- phase DNA Synthesis) the manufacture of genetic material.
The cellular division phase (M-phase) only.
2. The cell in cleavage does not go through a first or second interval (G1,G2 phases)
as in the normal cell cycle.
3. The cell division is regular at begging and then followed by multiple divisions that
cannot be pursued.
4. The cleavage leads to a multicellular early embryo
Cleavage character
Fig. Comparing between cell cycle in
early cleavage and somatic cell.
9. 5. Cleavage does not include an increase in the size of the fetus (about the same size
as the ovum ).
6. The embryonic form remains as it is the general form is the same (except the
appearance of the blastula cavity) At the end of the cleavage.
7. The time of the cleavage does not depend on the amount of the yolk, it varies by
the difference of living organisms:
• In the mammals' ova the first cleavage stage takes 24 hours which is the lowest
eggs in the yolk.
• In the amphibians 1st cleavage done within 3 hours.
• In the embryo of birds completes the stages of cleavage before the female lays the
egg.
Cleavage character
10. 1. The first level of splinting passes through the main axis of the fertilized ova starting
from the animal pole (the location of the nucleus to the vegetative pole (produces two
cells or two blastomers).
2. The second level of cleavage: Radial cleavage may be perpendicular to the first
division such as sea urchin and amphibians in both blastomers, and may be rotating
(Rotational cleavage) divided on the axis length and the other on the width of the axis
as in mammals.
3. The third level of splinting: horizontal on the first and second splits and produces
eight cell or blastomers upper group of the animal half pole of the embryo (in which
the nucleus was located) a lower group of the half of the vegetative pole (as in
amphibians)
4. The fourth cleavage: compose from 4 divisions in the upper cell group and another in
the lower cell group produces 16 cells.
5. The fifth cleavage is a set of 16 cell divisions in each blastomear to produce 32 cells
6. The sixth cleavage: a set of 32 cell division to give the stage of Morula.
7. The seventh cleavage is a set of divisions resulting in the blastula stage. the end of
the cleavage stage
Cleavage Levels
11. Radial cleavage Rotational cleavage
Type of cleavage, Radial cleavage in sea urchin embryo And Rotational cleavage as in mammals.
12. Cleavage differs from normal mitoses in 2 respects
1. Blastomeres do not grow in size between successive cell divisions as they do
in most cells. This leads to a rapid increase in the nucleus / cytoplasmic
ratio.
2. Cells undergoing cleavage have mainly S and M phases of the cell cycle
(little or no G1 or G2).
3. Cleavage occurs very rapidly, and mitosis and cytokinesis in each round of
cell division are complete within an hour. Typical somatic cells divide much
more slowly (several hours to days) and even the fastest cancer cells divide
much slower than occurs in a zygote during cleavage.
4. Cleavage differs in different types of eggs. The presence of large amounts of
yolk alters the cleavage pattern, leading to incomplete cleavage that
characterizes birds and reptiles.
13. Eggs are classified by how much yolk is present
1. Isolecithal eggs (iso = equal) have a small amount of yolk that is equally
distributed in the cytoplasm (most mammals have isolecithal eggs).
2. Mesolecithal eggs (meso = middle) have a moderate amount of yolk, and the
yolk is present mainly in the vegetal hemisphere (amphibians have
mesolecithal eggs).
3. Telolecithal eggs (telo = end) have a large amount of yolk that fills the
cytoplasm, except for a small area near the animal pole (fish, reptiles, and
birds).
4. Centrolecithal eggs have a lot of yolk that is concentrated within the center of
the cell (insects and arthropods).
Two areas of interest:
How does the process of cleavage differ in different organisms?
What mechanisms regulate cleavage?
14. The pattern of cleavage of the zygote depends upon the pattern of
yolk distribution.
1. Holoblastic cleavage occurs in isolecithal eggs (mammals, sea urchins). The
entire egg is cleaved during each division.
2. Meroblastic cleavage occurs when eggs have a lot of yolk. The egg does not
divide completely at each division. Two types:
a. Discoidal cleavage is limited to a small disc of cytoplasm at the animal
pole. All of the yolk filled cytoplasm fails to cleave (characteristic of
telolecithal eggs such as birds).
b. Superficial cleavage is limited to a thin surface area of cytoplasm that
covers the entire egg. The inside of the egg that is filled with yolk fails to
cleave (centrolecithal eggs such as insects).
16. • In eggs where the yolk is distributed regularly and equally,
such as most mammals, sea urchins.
• The amount of the yolk is small
• The cells is totally divided
• The whole cells are equal in size (cells in the animal pole are
smaller).
sea urchins. cleavage
1- Holoblastic equal cleavage
17. Amphibians have mesolecithal or telolecithal eggs and
undergo holoblastic cleavage with unequal blastomeres
Amphibian eggs have a lot of yolk, however, they are still able to undergo holoblastic
cleavage, but produce unequal blastomeres
The 1st cleavage is meridional, as is the 2nd.
The 3rd cleavage is equatorial. The cleavage is displaced toward the animal pole due to
the yolk. This results in 4 small animal blastomeres and 4 large vegetal blastomeres.
Morula (morum = mulberry) at the 16 to 32 cell stage the embryo is called a morula
because it looks like a mulberry. Witch will transfer to blastula stage.
18. The blastula formation = the last stage of cleavage
Blastula = will form at the end of cleavage stage, it from the 128 cell stage
onward the amphibian embryo is a blastula.
Its has a cavity called the blastocoel between the animal and vegetal pole.
The outer surface of the amphibian blastula has cells connected by specialized
cell junctions.
1. Tight junctions create a seal that isolates the outside of an embryo from the
inner layer. Tight junctions polarize the apical and basal surfaces. The basal
portions of cells start secreting into the blastocoel.
2. Desmosomes attach the blastomeres together on the outside.
3. Gap junctions connect all surface blastomeres.
20. In eggs with a large amount of yolk, the levels of cell division or cleavage
do not pass through cells and there are two types:
a- Discoidal
Is incomplete as in reptiles and birds where the cell division is confined to
the highest area of the egg. Where the fertilization occurs in the active
cytoplasm (germinal disk). (or blastoderm -cytoplasm free of the yolk and
floats on it - in the form of a small disk)
b - Partial cleavage ( or Meroblastic)
It is incomplete cell division or cell spliting as in fish where the cleavage
occurs at the top of the egg or some of the surface dividing cell are
complete, while the underneath dividing cells are incomplete, and
connected with the yolk.
2- Discoidal or Meroblastic Cleavage
23. 3-Superficial Cleavage
Superficial Cleavage occur in
insect
The Yolk in the central of the
ova
The several cleavage hapend
in the nuclouse And the
neclous moved to the
cytoplasm area.
In the surface of the ovum
and each neoulous suronded
by cytoplasm.
The cells form in the surface
of the egg.
24. • The researchers were able to draw a virtual map on the phase of the blastula
stage.
• The blastula are distributed into 3 areas (animal pole, vegetal pole and the
middle of the blastula), they follow the fate of each particular group of areas
of the blastula when they grow up later.
• The researcher (Vogt) were able to drown the fate map by special vital die
to stain the 3 area of blastula stage (its not toxic to the embryo cell), (the
vital die Neutral red, Nile blue sulphate, and Janus green),then they follow
the fate of each stained group of cell.
1. The animal pole will develop to the ectoderm witch will give the neural
tube and the skin (epidermis).
2. The vegetal pole (the lower part of the blastula) will form the endoderm
witch will develop and give the elementary canal,lungs.
3. The middle of the blastula will give the mesoderm that will develop and
give the dermis, heart, muscles, bones, kidney. See fig.
Fate Maps