The active site of an enzyme is the region that binds substrates and contains catalytic groups that directly participate in bond making and breaking. It takes the form of a cleft or pocket formed by amino acid residues far apart in the primary structure. The active sites of multimeric enzymes are located at interfaces between subunits and recruit residues from multiple monomers. Enzymes use an induced-fit or lock-and-key model to bind substrates specifically through weak interactions like hydrogen bonds and van der Waals forces at their active sites.
Ribosomal ribonucleic acid (rRNA) is a type of non-coding RNA that is the primary component of ribosomes and essential for protein synthesis. rRNA is transcribed from ribosomal DNA and binds with ribosomal proteins to form the small and large ribosomal subunits. rRNA folds into stem loops that allow it to interact with mRNA and tRNA to catalyze the translation of mRNA into proteins. rRNA makes up about 80% of cellular RNA and plays critical roles in the ribosome's peptidyl transferase activity through conserved sequences and structures.
Linkage refers to the presence of two different genes on the same chromosome . Two genes that occur on the same chromosome are said to be linked, and those that occur very close together are tightly linked.
Genetic recombination involves the breaking and rejoining of DNA to form new combinations of genes. It occurs primarily during meiosis through several types of recombination, including homologous recombination where DNA exchanges occur between similar DNA molecules. This increases genetic diversity and allows for traits to be mixed. Recombination benefits populations by generating variety among offspring and allowing deleterious genes to be removed without losing the entire chromosome. It has applications in cloning, mapping genes, and making transgenic organisms.
Structure and function of Messenger RNA (mRNA )ICHHA PURAK
This presentation of 42 slides delivers information about structure,function synthesis , life span of both prokaryotic and eukaryotic messenger RNA also about role in protein sorting and targetting
The human mitochondrial genome is much smaller than the nuclear genome, consisting of 16,569 base pairs. It contains 37 genes, 13 of which code for proteins involved in cellular respiration. Mitochondrial DNA is inherited solely from the mother and encodes for transfer RNA, ribosomal RNA and proteins that are critical subunits of the oxidative phosphorylation complexes. The human mitochondrial genome has a highly condensed structure with minimal non-coding regions and some overlapping genes. It also differs slightly from the standard genetic code.
The Calvin cycle occurs in two stages: carbon fixation and reduction. During carbon fixation, the enzyme RuBisCO incorporates carbon from CO2 into RuBP to form unstable 6-carbon compounds. These 6-carbon compounds are then split into two 3-carbon molecules which are reduced using electrons from NADPH and energy from ATP. The 3-carbon molecules are then rearranged to regenerate RuBP, completing the cycle. The Calvin cycle uses energy from the light reactions in the form of ATP and NADPH along with CO2 to produce organic compounds like glucose.
The active site of an enzyme is the region that binds substrates and contains catalytic groups that directly participate in bond making and breaking. It takes the form of a cleft or pocket formed by amino acid residues far apart in the primary structure. The active sites of multimeric enzymes are located at interfaces between subunits and recruit residues from multiple monomers. Enzymes use an induced-fit or lock-and-key model to bind substrates specifically through weak interactions like hydrogen bonds and van der Waals forces at their active sites.
Ribosomal ribonucleic acid (rRNA) is a type of non-coding RNA that is the primary component of ribosomes and essential for protein synthesis. rRNA is transcribed from ribosomal DNA and binds with ribosomal proteins to form the small and large ribosomal subunits. rRNA folds into stem loops that allow it to interact with mRNA and tRNA to catalyze the translation of mRNA into proteins. rRNA makes up about 80% of cellular RNA and plays critical roles in the ribosome's peptidyl transferase activity through conserved sequences and structures.
Linkage refers to the presence of two different genes on the same chromosome . Two genes that occur on the same chromosome are said to be linked, and those that occur very close together are tightly linked.
Genetic recombination involves the breaking and rejoining of DNA to form new combinations of genes. It occurs primarily during meiosis through several types of recombination, including homologous recombination where DNA exchanges occur between similar DNA molecules. This increases genetic diversity and allows for traits to be mixed. Recombination benefits populations by generating variety among offspring and allowing deleterious genes to be removed without losing the entire chromosome. It has applications in cloning, mapping genes, and making transgenic organisms.
Structure and function of Messenger RNA (mRNA )ICHHA PURAK
This presentation of 42 slides delivers information about structure,function synthesis , life span of both prokaryotic and eukaryotic messenger RNA also about role in protein sorting and targetting
The human mitochondrial genome is much smaller than the nuclear genome, consisting of 16,569 base pairs. It contains 37 genes, 13 of which code for proteins involved in cellular respiration. Mitochondrial DNA is inherited solely from the mother and encodes for transfer RNA, ribosomal RNA and proteins that are critical subunits of the oxidative phosphorylation complexes. The human mitochondrial genome has a highly condensed structure with minimal non-coding regions and some overlapping genes. It also differs slightly from the standard genetic code.
The Calvin cycle occurs in two stages: carbon fixation and reduction. During carbon fixation, the enzyme RuBisCO incorporates carbon from CO2 into RuBP to form unstable 6-carbon compounds. These 6-carbon compounds are then split into two 3-carbon molecules which are reduced using electrons from NADPH and energy from ATP. The 3-carbon molecules are then rearranged to regenerate RuBP, completing the cycle. The Calvin cycle uses energy from the light reactions in the form of ATP and NADPH along with CO2 to produce organic compounds like glucose.
A suppressor mutation counters the effects of an original mutation by restoring the wild-type phenotype. There are two main types of suppressor mutations: intragenic mutations occur within the same gene and restore function through alternate amino acid substitutions, while intergenic mutations occur elsewhere in the genome and restore function through interacting gene products. Suppressor mutations are useful for studying protein-protein interactions and dissecting biological pathways.
Chloroplasts are organelles found in plant cells and algae that conduct photosynthesis. They have their own DNA and can synthesize some of their own proteins, making them semi-autonomous. Chloroplasts contain chlorophyll and carotenoids which capture light energy. Their internal structure includes an envelope, stroma, and thylakoids where the light reactions take place. It is believed that chloroplasts originated through endosymbiosis between cyanobacteria and eukaryotic cells. The two main stages of photosynthesis are the light reactions on the thylakoid membranes which produce ATP and NADPH, and the dark reactions in the stroma that use these products to fix carbon into sugars.
The document summarizes the organization and structure of DNA within chromosomes. It discusses how DNA is packaged at different levels, from winding around histones to form nucleosomes, to coiling to form the 30nm chromatin fiber and further condensing to form mitotic chromosomes. It also describes the centromeres and telomeres, which play important roles in chromosome segregation and stability. Chromosomal banding patterns allow distinguishing each chromosome.
CAM plants fix carbon dioxide at night and store it as malic acid in their vacuoles. During the day, the stomata remain closed while the malic acid releases its carbon dioxide within the chloroplasts for photosynthesis. This two-part cycle allows CAM plants to conserve water by closing their stomata during the heat of the day. Key benefits of CAM include adaptations for arid conditions like thick cuticles and leaves and the ability to minimize water loss through transpiration.
This power point presentation consisting of 41 slides is an attempt to describe what is photorespiration,major photorespiratory pathway in C3 plants ,why photorespiration doesnot take place in C4 plants,structure of Rubisco enzyme ,difference between Photorespiration and Dark respiration and Significance of Photorespiration
This document discusses chloroplast DNA (cpDNA). Chloroplasts contain their own circular genome of double-stranded DNA ranging from 140-200kb. The cpDNA contains genes that code for proteins involved in photosynthesis as well as rRNA and tRNA. It has a quadripartite structure containing single copy and inverted repeat regions. Tobacco and liverwort were two of the first chloroplast genomes to be sequenced. Molecular studies of cpDNA regions have been useful for plant systematics. Replication of cpDNA is independent of nuclear DNA and involves enzymes like DNA polymerase and helicase.
Ribosomes are cell structures composed of RNA and proteins that synthesize proteins. They were discovered in plant and animal cells in the 1950s. Ribosomes can be found floating in the cytoplasm or attached to the endoplasmic reticulum, and their location determines whether proteins are used inside or outside the cell. Ribosomes consist of two subunits that come together to translate mRNA into proteins according to the genetic code.
Plasmodesmata are narrow strands of cytoplasm that connect adjacent plant cells and allow for transport of substances between cells. They were first observed under light microscopes in 1879 but required electron microscopes to confirm their nature as cytoplasmic strands. Plasmodesmata contain a plasma membrane-lined channel and a desmotubule made of tightly constricted endoplasmic reticulum. Substances move between cells through the region between the desmotubule and plasma membrane, called the cytoplasmic sleeve. Plasmodesmata are either primary, formed during cell division, or secondary, formed across existing cell walls.
DNA is the genetic material found in the nucleus of eukaryotic cells and in the chromosomes of prokaryotes. It exists in several forms, including linear chromosomes in eukaryotes and circular chromosomes in prokaryotes and organelles. DNA is made up of a double helix structure stabilized by hydrogen bonding between complementary nucleotide base pairs. The structure of DNA allows it to efficiently store and transmit genetic information.
Gene protein relattionship. genetic fine structureJannat Iftikhar
1. The document discusses how genes work through the one-gene-one-enzyme hypothesis, which provides that each gene is responsible for producing a single enzyme.
2. It also covers the relationship between genes, proteins, and phenotypes. Mutations in a single nucleotide can alter protein function, as shown through studies of hemoglobin.
3. Experiments by Seymour Benzer using bacteriophage T4 helped disprove the "bead theory" by showing that the smallest units of mutation and recombination are single nucleotide pairs through analysis of point mutations and deletions within the rII gene.
This document summarizes the C3 and C4 cycles. The C3 cycle (also called the Calvin cycle) fixes carbon dioxide into three-carbon compounds like phosphoglyceric acid in three stages: fixation, reduction, and regeneration. The C4 cycle is an alternative pathway that fixes carbon dioxide into four-carbon compounds like oxaloacetic acid. It occurs in plants like maize and sorghum that show a distinct Kranz anatomy with bundle sheath cells surrounding vascular bundles. The C4 cycle involves two carboxylation steps - the first in mesophyll cell chloroplasts and the second in bundle sheath cell chloroplasts.
Sulphate assimilation which takes place mainly in chloroplasts in higher plants leads to the formation of cysteine. cysteine is the central compound in sulphur assimilation.
Introduction And Classification
Anatomy Of Flower
Life Cycle Of Arabidopsis
Early Flower Development
Embryogenesis-
A. Formation Of Microspores
B. Formation Of Megaspores
Embryonic Development Starts By Establishing A Root-shoot Axis And Then Halts Inside The Seed
Arabidopsis Genome Is Rich In Developmental Control Genes.
Control Of Carpel & Fruit Development
Arabidopsis Thaliana A Model Plant
Conclusion
References
CELL CYCLE , MITOSIS ,MEIOSIS AND CELL REGULATIONLIFE SCIENCES
The document discusses the cell cycle and its regulation. It describes the main phases of the cell cycle including interphase with G1, S, and G2 phases, and mitosis. It also covers meiosis and the key differences between mitosis and meiosis. Cell cycle checkpoints are mentioned which allow the cell cycle to be halted at certain points if conditions are not favorable for progression.
Theories regarding origin of Mitochondria and ChloroplastsGuttiPavan
The document summarizes the endosymbiotic theory of the origin of mitochondria and chloroplasts. It states that mitochondria likely evolved from aerobic prokaryotes that were engulfed by early eukaryotic cells, eventually becoming specialized organelles. Similarly, chloroplasts may have evolved from photosynthetic prokaryotes that were engulfed by eukaryotic cells already containing mitochondria. This endosymbiotic theory was first proposed by Lynn Margulis and has received significant evidentiary support.
The document summarizes the Calvin cycle, which uses energy from the light-dependent reaction to convert carbon dioxide into carbohydrates. It occurs in the stroma and uses ATP and NADPH produced during the light reaction. The Calvin cycle consists of carbon fixation, reduction, and regeneration steps to reduce carbon dioxide into glyceraldehyde-3-phosphate, which can then be converted into glucose or other carbohydrates.
ultra structure of Ribosome, Prokaryotic Ribosome, Eukaryotic Ribosome, Svedberg unit, Centrifugal force, assembly of Ribosome, functions of Ribosome, models of Ribosomes, fine structure of Ribosome, Discovery of Ribosome,
Thermodynamic laws describe the flows and interchanges of heat, energy and matter.
Almost all chemical and biochemical processes are as a result of transformation of energy.
Laws can provide important insights into metabolism and bioenergetics.
The energy exchanges between the system and the surroundings balance each other.
There is a hierarchy of energetics among organisms
transfection and invitro packaging of phage genomeNOMI KhanS
There are two main methods for introducing recombinant phage DNA into bacterial cells: transfection and in vitro packaging. Transfection involves mixing purified phage DNA with competent bacterial cells and inducing DNA uptake via heat shock. In vitro packaging involves using proteins from defective phage strains to package recombinant lambda DNA into phage particles outside of cells. These phage particles can then infect bacterial cells. Recombinant phages can be identified by their ability to form clear plaques on indicator plates due to inactivation of genes like lacZ or CI upon insertion of foreign DNA.
Cell differentiation begins with undifferentiated cells that can become any cell type. Gene expression and environmental signals determine each cell's specialization. While all cells contain the same DNA, different genes are activated in different cell types. Specialized cells like nerve, muscle, and blood cells have unique structures and functions. Stem cells can renew themselves and differentiate into specialized cell types, making them useful for research and medical applications like treating disease and injury. Embryonic stem cells come from embryos and adult stem cells reside in tissues, with induced pluripotent stem cells generated from adult cells.
1. Embryonic development involves cell proliferation to increase cell numbers through cleavage and cell differentiation to form different cell types through determination.
2. Gene expression, cell communication, hormones, and environmental factors control this process by regulating proliferation and differentiation.
3. Early embryonic development progresses from a single zygote to a complex organism through regulated cell proliferation, differentiation, and morphogenesis.
A suppressor mutation counters the effects of an original mutation by restoring the wild-type phenotype. There are two main types of suppressor mutations: intragenic mutations occur within the same gene and restore function through alternate amino acid substitutions, while intergenic mutations occur elsewhere in the genome and restore function through interacting gene products. Suppressor mutations are useful for studying protein-protein interactions and dissecting biological pathways.
Chloroplasts are organelles found in plant cells and algae that conduct photosynthesis. They have their own DNA and can synthesize some of their own proteins, making them semi-autonomous. Chloroplasts contain chlorophyll and carotenoids which capture light energy. Their internal structure includes an envelope, stroma, and thylakoids where the light reactions take place. It is believed that chloroplasts originated through endosymbiosis between cyanobacteria and eukaryotic cells. The two main stages of photosynthesis are the light reactions on the thylakoid membranes which produce ATP and NADPH, and the dark reactions in the stroma that use these products to fix carbon into sugars.
The document summarizes the organization and structure of DNA within chromosomes. It discusses how DNA is packaged at different levels, from winding around histones to form nucleosomes, to coiling to form the 30nm chromatin fiber and further condensing to form mitotic chromosomes. It also describes the centromeres and telomeres, which play important roles in chromosome segregation and stability. Chromosomal banding patterns allow distinguishing each chromosome.
CAM plants fix carbon dioxide at night and store it as malic acid in their vacuoles. During the day, the stomata remain closed while the malic acid releases its carbon dioxide within the chloroplasts for photosynthesis. This two-part cycle allows CAM plants to conserve water by closing their stomata during the heat of the day. Key benefits of CAM include adaptations for arid conditions like thick cuticles and leaves and the ability to minimize water loss through transpiration.
This power point presentation consisting of 41 slides is an attempt to describe what is photorespiration,major photorespiratory pathway in C3 plants ,why photorespiration doesnot take place in C4 plants,structure of Rubisco enzyme ,difference between Photorespiration and Dark respiration and Significance of Photorespiration
This document discusses chloroplast DNA (cpDNA). Chloroplasts contain their own circular genome of double-stranded DNA ranging from 140-200kb. The cpDNA contains genes that code for proteins involved in photosynthesis as well as rRNA and tRNA. It has a quadripartite structure containing single copy and inverted repeat regions. Tobacco and liverwort were two of the first chloroplast genomes to be sequenced. Molecular studies of cpDNA regions have been useful for plant systematics. Replication of cpDNA is independent of nuclear DNA and involves enzymes like DNA polymerase and helicase.
Ribosomes are cell structures composed of RNA and proteins that synthesize proteins. They were discovered in plant and animal cells in the 1950s. Ribosomes can be found floating in the cytoplasm or attached to the endoplasmic reticulum, and their location determines whether proteins are used inside or outside the cell. Ribosomes consist of two subunits that come together to translate mRNA into proteins according to the genetic code.
Plasmodesmata are narrow strands of cytoplasm that connect adjacent plant cells and allow for transport of substances between cells. They were first observed under light microscopes in 1879 but required electron microscopes to confirm their nature as cytoplasmic strands. Plasmodesmata contain a plasma membrane-lined channel and a desmotubule made of tightly constricted endoplasmic reticulum. Substances move between cells through the region between the desmotubule and plasma membrane, called the cytoplasmic sleeve. Plasmodesmata are either primary, formed during cell division, or secondary, formed across existing cell walls.
DNA is the genetic material found in the nucleus of eukaryotic cells and in the chromosomes of prokaryotes. It exists in several forms, including linear chromosomes in eukaryotes and circular chromosomes in prokaryotes and organelles. DNA is made up of a double helix structure stabilized by hydrogen bonding between complementary nucleotide base pairs. The structure of DNA allows it to efficiently store and transmit genetic information.
Gene protein relattionship. genetic fine structureJannat Iftikhar
1. The document discusses how genes work through the one-gene-one-enzyme hypothesis, which provides that each gene is responsible for producing a single enzyme.
2. It also covers the relationship between genes, proteins, and phenotypes. Mutations in a single nucleotide can alter protein function, as shown through studies of hemoglobin.
3. Experiments by Seymour Benzer using bacteriophage T4 helped disprove the "bead theory" by showing that the smallest units of mutation and recombination are single nucleotide pairs through analysis of point mutations and deletions within the rII gene.
This document summarizes the C3 and C4 cycles. The C3 cycle (also called the Calvin cycle) fixes carbon dioxide into three-carbon compounds like phosphoglyceric acid in three stages: fixation, reduction, and regeneration. The C4 cycle is an alternative pathway that fixes carbon dioxide into four-carbon compounds like oxaloacetic acid. It occurs in plants like maize and sorghum that show a distinct Kranz anatomy with bundle sheath cells surrounding vascular bundles. The C4 cycle involves two carboxylation steps - the first in mesophyll cell chloroplasts and the second in bundle sheath cell chloroplasts.
Sulphate assimilation which takes place mainly in chloroplasts in higher plants leads to the formation of cysteine. cysteine is the central compound in sulphur assimilation.
Introduction And Classification
Anatomy Of Flower
Life Cycle Of Arabidopsis
Early Flower Development
Embryogenesis-
A. Formation Of Microspores
B. Formation Of Megaspores
Embryonic Development Starts By Establishing A Root-shoot Axis And Then Halts Inside The Seed
Arabidopsis Genome Is Rich In Developmental Control Genes.
Control Of Carpel & Fruit Development
Arabidopsis Thaliana A Model Plant
Conclusion
References
CELL CYCLE , MITOSIS ,MEIOSIS AND CELL REGULATIONLIFE SCIENCES
The document discusses the cell cycle and its regulation. It describes the main phases of the cell cycle including interphase with G1, S, and G2 phases, and mitosis. It also covers meiosis and the key differences between mitosis and meiosis. Cell cycle checkpoints are mentioned which allow the cell cycle to be halted at certain points if conditions are not favorable for progression.
Theories regarding origin of Mitochondria and ChloroplastsGuttiPavan
The document summarizes the endosymbiotic theory of the origin of mitochondria and chloroplasts. It states that mitochondria likely evolved from aerobic prokaryotes that were engulfed by early eukaryotic cells, eventually becoming specialized organelles. Similarly, chloroplasts may have evolved from photosynthetic prokaryotes that were engulfed by eukaryotic cells already containing mitochondria. This endosymbiotic theory was first proposed by Lynn Margulis and has received significant evidentiary support.
The document summarizes the Calvin cycle, which uses energy from the light-dependent reaction to convert carbon dioxide into carbohydrates. It occurs in the stroma and uses ATP and NADPH produced during the light reaction. The Calvin cycle consists of carbon fixation, reduction, and regeneration steps to reduce carbon dioxide into glyceraldehyde-3-phosphate, which can then be converted into glucose or other carbohydrates.
ultra structure of Ribosome, Prokaryotic Ribosome, Eukaryotic Ribosome, Svedberg unit, Centrifugal force, assembly of Ribosome, functions of Ribosome, models of Ribosomes, fine structure of Ribosome, Discovery of Ribosome,
Thermodynamic laws describe the flows and interchanges of heat, energy and matter.
Almost all chemical and biochemical processes are as a result of transformation of energy.
Laws can provide important insights into metabolism and bioenergetics.
The energy exchanges between the system and the surroundings balance each other.
There is a hierarchy of energetics among organisms
transfection and invitro packaging of phage genomeNOMI KhanS
There are two main methods for introducing recombinant phage DNA into bacterial cells: transfection and in vitro packaging. Transfection involves mixing purified phage DNA with competent bacterial cells and inducing DNA uptake via heat shock. In vitro packaging involves using proteins from defective phage strains to package recombinant lambda DNA into phage particles outside of cells. These phage particles can then infect bacterial cells. Recombinant phages can be identified by their ability to form clear plaques on indicator plates due to inactivation of genes like lacZ or CI upon insertion of foreign DNA.
Cell differentiation begins with undifferentiated cells that can become any cell type. Gene expression and environmental signals determine each cell's specialization. While all cells contain the same DNA, different genes are activated in different cell types. Specialized cells like nerve, muscle, and blood cells have unique structures and functions. Stem cells can renew themselves and differentiate into specialized cell types, making them useful for research and medical applications like treating disease and injury. Embryonic stem cells come from embryos and adult stem cells reside in tissues, with induced pluripotent stem cells generated from adult cells.
1. Embryonic development involves cell proliferation to increase cell numbers through cleavage and cell differentiation to form different cell types through determination.
2. Gene expression, cell communication, hormones, and environmental factors control this process by regulating proliferation and differentiation.
3. Early embryonic development progresses from a single zygote to a complex organism through regulated cell proliferation, differentiation, and morphogenesis.
The document discusses the cell cycle and differentiation. It describes the three main stages of the cell cycle as interphase, mitosis, and cytokinesis. Interphase is broken down into Gap 1, synthesis, and Gap 2 phases. Mitosis consists of prophase, metaphase, anaphase, and telophase. Cytokinesis is the final stage where the cell physically divides. Differentiation is when unspecialized cells become specialized cell types by turning on and off parts of their DNA. Stem cells can become any cell type while progenitor cells are somewhat specialized.
Cell Introduction and Cell Differentiationpaigesirois
1) The cell is the basic structural and functional unit of all living organisms. Cells were first observed by Robert Hooke and Anton van Leeuwenhoek in the 1600s.
2) The Cell Theory states that all living things are made of cells, cells are the basic unit of life, and new cells are produced from existing cells. It also includes that energy flows through cells and heredity information is passed from cell to cell.
3) Cellular differentiation is the process where less specialized cells mature and develop into more distinct cell types with specialized shapes, sizes and functions that make up complex multi-system organisms.
Multicellular organisms are composed of many different types of cells that cooperate and communicate to form organized systems. In humans there are over 10 trillion cells comprising 200 types of tissues. Each cell originates from a single fertilized egg cell and differentiates through activation of certain genes while others remain inactive, allowing cells to perform specialized functions. Bacteria also regulate genes in response to environmental signals, turning groups on and off. The lac operon in E. coli is a well-studied example of genetic control, where a repressor protein either allows or prevents transcription of genes involved in lactose metabolism depending on the presence of lactose.
Cell-cell interaction refers to direct interactions between cell surfaces that allow cells to communicate and respond to changes. These interactions involve stable cell junctions that provide adhesion within tissues and control cell shape and function. The main types of cell junctions are tight junctions, adherens junctions, desmosomes, and gap junctions. Tight junctions prevent movement of molecules between cells and form a selective barrier. Adherens junctions and desmosomes provide strength and signaling between cells. Gap junctions allow small molecules to pass directly between cells, allowing cell-cell communication. The loss of these cell-cell interactions can result in uncontrolled cell growth and cancer.
The document summarizes key aspects of culturing cells outside of their native biological environment. Cultured cells experience changes to their microenvironment, cell-cell interactions, and exposure to stimuli. Their growth is influenced by factors like the substrate, medium composition, temperature, and gas phase. Most cells require attachment to a substrate to proliferate. Adhesion molecules like integrins and cadherins mediate attachment and formation of intercellular junctions. The extracellular matrix and cytoskeleton also influence cell behavior. Control of the cell cycle, proliferation, differentiation, motility, and response to the culture environment are described at a high level. Challenges like dedifferentiation and evolution of cell lines over multiple passages are also covered.
Cell junctions connect neighboring cells and classify into occluding, communicating, and anchoring junctions. Occluding junctions like tight junctions prevent molecules from passing between cells. Communicating junctions like gap junctions allow small molecules to pass directly between cells, allowing cell-to-cell communication. Anchoring junctions provide structural strength and attachment between cells or between cells and the extracellular matrix. Major anchoring junctions include desmosomes and hemidesmosomes.
The document discusses cell membranes. It describes how cell membranes are made up of lipids like phospholipids and cholesterol that form a bilayer. Proteins are also embedded throughout membranes and help regulate transport of molecules. The membrane acts as a selective barrier, controlling what enters and exits the cell using transport proteins. Membranes surround not just cells but also internal organelles, and their compositions vary by location to support different functions.
- Histology is the study of tissues and how they form organs. It examines tissues at a microscopic level.
- Cytology is the study of cells. There are two main cell types - prokaryotic cells which are small and lack organelles, and eukaryotic cells which exist primarily in multicellular organisms and have organelles.
- The cell membrane forms the boundary of the cell and regulates what enters and exits. It contains proteins, lipids, and carbohydrates. It performs important functions like acting as a barrier, having receptor sites, and facilitating transport processes into and out of the cell.
Plant systems: Extracellular matrix components of plants-cell wall, cellulose and hemicelluloses, extensins, WAKs, secondary wall structure, pits-primary and secondary pits and their development, plasmodesmota-structure and functions, pectins, cutins, lignins, turnover of cell wall components
The document discusses experiments where embryos are disaggregated into individual cells. When the cells are recombined, they only partially reorganize and do not fully reform an embryo. This suggests that genes only provide partial instructions for assembly and that cell adhesion is also important. When different cell types are combined, they aggregate based on their relative adhesive properties, with the most adhesive cells forming the core. The document concludes that cell adhesion, not just genes, is a major factor controlling cell position and morphogenesis.
This document summarizes different types of cell adhesion molecules (CAMs). It discusses cadherins, which are the primary CAMs in adherens junctions and desmosomes. Integrins are heterodimeric receptors that connect the intracellular and extracellular environments and are involved in cell adhesion to the extracellular matrix. The immunoglobulin superfamily of CAMs are calcium-independent transmembrane proteins with immunoglobulin-like domains. Selectins mediate the initial tethering of leukocytes to endothelial cells during inflammation. Cell adhesion molecules play important roles in processes like embryogenesis, immunity, tissue development, and cancer metastasis.
Morphogenesis is the process by which organisms develop their shape through cellular processes like cell movement, division, death and changes in shape. These cellular movements collectively called morphogenetic movements play an important role in shaping developing embryos. Key cellular processes in morphogenesis include cell division, changes in shape and size, fusion, and death. Additional important processes are cell-matrix and cell-cell interactions mediated by cell adhesion molecules which allow cells to organize into tissues through differential adhesion properties. Differences in adhesion between cell types cause them to self-organize into patterns through thermodynamic principles.
Intercellular connections and molecular motorsAnwar Siddiqui
This document summarizes a physiology seminar on intercellular connections and molecular motors. It discusses various cell adhesion molecules like cadherins, selectins, immunoglobulin superfamily molecules, and integrins that mediate cell-cell and cell-matrix adhesion. It also describes different types of intercellular junctions such as tight junctions, desmosomes, and hemidesmosomes. Finally, it provides an overview of molecular motors like kinesin, dynein, and myosin that transport cargo within cells and generate forces through ATP hydrolysis.
Gap junctions allow direct communication between adjacent cells by forming channels between the cells' cytoplasm. The structure of a gap junction consists of connexons - cylinders of six transmembrane protein subunits called connexins - arranged back-to-back between the plasma membranes of adjacent cells. These connexons join to form channels about 1.5-2.0 nm in diameter that connect the cytoplasm of the two cells and allow small molecules and ions to pass directly between cells, enabling both electrical and metabolic cooperation.
Cell junctions are multiprotein complexes that provide contact between adjacent animal cells and help hold tissues together. The main types are anchoring junctions, which attach cells to surrounding matrix and each other; gap junctions, which allow direct communication between cells; and tight junctions, which form barriers between epithelial layers. Cell junction molecules like selectins, cadherins, integrins, and the immunoglobulin superfamily mediate cell-cell adhesion through calcium-dependent and calcium-independent binding of domains on neighboring cell surfaces. Cell junctions are essential for tissue structure, cell signaling, and barrier functions in the body.
This presentation intends to explore the communication of the cell within and others for sustainability along the regulation mechanisms by the cellular neural networks and others to sing the song of the life.
Intercellular junctions in Health and Diseasereshma545193
1. Tight junctions, desmosomes, and focal adhesions are the three main types of intercellular junctions that were discussed.
2. Tight junctions form a seal around cells to control passage between cells and maintain cell polarity. Desmosomes anchor cells together through intermediate filaments to resist mechanical stress. Focal adhesions link the actin cytoskeleton to the extracellular matrix through integrin proteins.
3. Diseases can arise from defects in the proteins that form intercellular junctions, disrupting the barriers and cell-cell or cell-matrix connections they provide.
Cell junctions , cell adhesion and extra cellular matrixMinali Singh
Cell junctions connect cells to each other and to the extracellular matrix through four main types: anchoring junctions, occluding junctions, channel-forming junctions, and signal-relaying junctions. Anchoring junctions include cadherins and integrins, which link cells together and attach cells to the extracellular matrix. Tight junctions and desmosomes form barriers and anchor cells via intermediate filaments. Gap junctions connect cell cytoplasm to allow communication through molecule and ion transfer. The extracellular matrix surrounds cells and is composed of collagen fibers and proteoglycans.
Cell junctions connect neighboring cells and the cell to the extracellular matrix. They are classified into occluding junctions, communicating junctions, and anchoring junctions. Cell adhesion molecules (CAMs) are important proteins that promote cell-cell and cell-matrix interactions through three domains: an extracellular domain that binds to other cells, a transmembrane domain, and a cytoplasmic domain connected to the cytoskeleton. CAMs can be divided into four major families: the cadherin superfamily, selectins, immunoglobulin superfamily, and integrins. Tight junctions form a tight seal between cells to prevent molecules from passing through. Gap junctions allow direct diffusion of ions and small molecules between adjacent cells through conn
This document provides information about cell structure and function. It begins by explaining that both prokaryotic and eukaryotic cells contain genetic material organized into chromosomes. It then describes the basic components and structures of cells, including membranes, organelles, cytoplasm, and the differences between prokaryotic and eukaryotic cells. The document goes on to explain the cell cycle, mitosis, and meiosis. It details the stages of mitosis and how genetic material is duplicated and divided equally between two daughter cells. It also briefly introduces meiosis and its role in sexual reproduction.
Cell junctions connect neighboring cells and classify into three main types - occluding, communicating, and anchoring junctions. Occluding junctions prevent molecules from passing between cells, like tight junctions. Communicating junctions allow transfer of substances between cells via channels, such as gap junctions. Anchoring junctions provide structural strength, exemplified by desmosomes attaching cells to each other or hemidesmosomes attaching cells to the extracellular matrix. Cell adhesion molecules like cadherins and selectins are transmembrane proteins that mediate cell-cell binding and participate in various cellular processes during development, wound healing, and immune responses.
Similar to Cell adhesion in plants and animals (20)
Microbial interaction
Microorganisms interacts with each other and can be physically associated with another organisms in a variety of ways.
One organism can be located on the surface of another organism as an ectobiont or located within another organism as endobiont.
Microbial interaction may be positive such as mutualism, proto-cooperation, commensalism or may be negative such as parasitism, predation or competition
Types of microbial interaction
Positive interaction: mutualism, proto-cooperation, commensalism
Negative interaction: Ammensalism (antagonism), parasitism, predation, competition
I. Mutualism:
It is defined as the relationship in which each organism in interaction gets benefits from association. It is an obligatory relationship in which mutualist and host are metabolically dependent on each other.
Mutualistic relationship is very specific where one member of association cannot be replaced by another species.
Mutualism require close physical contact between interacting organisms.
Relationship of mutualism allows organisms to exist in habitat that could not occupied by either species alone.
Mutualistic relationship between organisms allows them to act as a single organism.
Examples of mutualism:
i. Lichens:
Lichens are excellent example of mutualism.
They are the association of specific fungi and certain genus of algae. In lichen, fungal partner is called mycobiont and algal partner is called
II. Syntrophism:
It is an association in which the growth of one organism either depends on or improved by the substrate provided by another organism.
In syntrophism both organism in association gets benefits.
Compound A
Utilized by population 1
Compound B
Utilized by population 2
Compound C
utilized by both Population 1+2
Products
In this theoretical example of syntrophism, population 1 is able to utilize and metabolize compound A, forming compound B but cannot metabolize beyond compound B without co-operation of population 2. Population 2is unable to utilize compound A but it can metabolize compound B forming compound C. Then both population 1 and 2 are able to carry out metabolic reaction which leads to formation of end product that neither population could produce alone.
Examples of syntrophism:
i. Methanogenic ecosystem in sludge digester
Methane produced by methanogenic bacteria depends upon interspecies hydrogen transfer by other fermentative bacteria.
Anaerobic fermentative bacteria generate CO2 and H2 utilizing carbohydrates which is then utilized by methanogenic bacteria (Methanobacter) to produce methane.
ii. Lactobacillus arobinosus and Enterococcus faecalis:
In the minimal media, Lactobacillus arobinosus and Enterococcus faecalis are able to grow together but not alone.
The synergistic relationship between E. faecalis and L. arobinosus occurs in which E. faecalis require folic acid
PPT on Sustainable Land Management presented at the three-day 'Training and Validation Workshop on Modules of Climate Smart Agriculture (CSA) Technologies in South Asia' workshop on April 22, 2024.
Candidate young stellar objects in the S-cluster: Kinematic analysis of a sub...Sérgio Sacani
Context. The observation of several L-band emission sources in the S cluster has led to a rich discussion of their nature. However, a definitive answer to the classification of the dusty objects requires an explanation for the detection of compact Doppler-shifted Brγ emission. The ionized hydrogen in combination with the observation of mid-infrared L-band continuum emission suggests that most of these sources are embedded in a dusty envelope. These embedded sources are part of the S-cluster, and their relationship to the S-stars is still under debate. To date, the question of the origin of these two populations has been vague, although all explanations favor migration processes for the individual cluster members. Aims. This work revisits the S-cluster and its dusty members orbiting the supermassive black hole SgrA* on bound Keplerian orbits from a kinematic perspective. The aim is to explore the Keplerian parameters for patterns that might imply a nonrandom distribution of the sample. Additionally, various analytical aspects are considered to address the nature of the dusty sources. Methods. Based on the photometric analysis, we estimated the individual H−K and K−L colors for the source sample and compared the results to known cluster members. The classification revealed a noticeable contrast between the S-stars and the dusty sources. To fit the flux-density distribution, we utilized the radiative transfer code HYPERION and implemented a young stellar object Class I model. We obtained the position angle from the Keplerian fit results; additionally, we analyzed the distribution of the inclinations and the longitudes of the ascending node. Results. The colors of the dusty sources suggest a stellar nature consistent with the spectral energy distribution in the near and midinfrared domains. Furthermore, the evaporation timescales of dusty and gaseous clumps in the vicinity of SgrA* are much shorter ( 2yr) than the epochs covered by the observations (≈15yr). In addition to the strong evidence for the stellar classification of the D-sources, we also find a clear disk-like pattern following the arrangements of S-stars proposed in the literature. Furthermore, we find a global intrinsic inclination for all dusty sources of 60 ± 20◦, implying a common formation process. Conclusions. The pattern of the dusty sources manifested in the distribution of the position angles, inclinations, and longitudes of the ascending node strongly suggests two different scenarios: the main-sequence stars and the dusty stellar S-cluster sources share a common formation history or migrated with a similar formation channel in the vicinity of SgrA*. Alternatively, the gravitational influence of SgrA* in combination with a massive perturber, such as a putative intermediate mass black hole in the IRS 13 cluster, forces the dusty objects and S-stars to follow a particular orbital arrangement. Key words. stars: black holes– stars: formation– Galaxy: center– galaxies: star formation
PPT on Direct Seeded Rice presented at the three-day 'Training and Validation Workshop on Modules of Climate Smart Agriculture (CSA) Technologies in South Asia' workshop on April 22, 2024.
Evidence of Jet Activity from the Secondary Black Hole in the OJ 287 Binary S...Sérgio Sacani
Wereport the study of a huge optical intraday flare on 2021 November 12 at 2 a.m. UT in the blazar OJ287. In the binary black hole model, it is associated with an impact of the secondary black hole on the accretion disk of the primary. Our multifrequency observing campaign was set up to search for such a signature of the impact based on a prediction made 8 yr earlier. The first I-band results of the flare have already been reported by Kishore et al. (2024). Here we combine these data with our monitoring in the R-band. There is a big change in the R–I spectral index by 1.0 ±0.1 between the normal background and the flare, suggesting a new component of radiation. The polarization variation during the rise of the flare suggests the same. The limits on the source size place it most reasonably in the jet of the secondary BH. We then ask why we have not seen this phenomenon before. We show that OJ287 was never before observed with sufficient sensitivity on the night when the flare should have happened according to the binary model. We also study the probability that this flare is just an oversized example of intraday variability using the Krakow data set of intense monitoring between 2015 and 2023. We find that the occurrence of a flare of this size and rapidity is unlikely. In machine-readable Tables 1 and 2, we give the full orbit-linked historical light curve of OJ287 as well as the dense monitoring sample of Krakow.
Immersive Learning That Works: Research Grounding and Paths ForwardLeonel Morgado
We will metaverse into the essence of immersive learning, into its three dimensions and conceptual models. This approach encompasses elements from teaching methodologies to social involvement, through organizational concerns and technologies. Challenging the perception of learning as knowledge transfer, we introduce a 'Uses, Practices & Strategies' model operationalized by the 'Immersive Learning Brain' and ‘Immersion Cube’ frameworks. This approach offers a comprehensive guide through the intricacies of immersive educational experiences and spotlighting research frontiers, along the immersion dimensions of system, narrative, and agency. Our discourse extends to stakeholders beyond the academic sphere, addressing the interests of technologists, instructional designers, and policymakers. We span various contexts, from formal education to organizational transformation to the new horizon of an AI-pervasive society. This keynote aims to unite the iLRN community in a collaborative journey towards a future where immersive learning research and practice coalesce, paving the way for innovative educational research and practice landscapes.
Signatures of wave erosion in Titan’s coastsSérgio Sacani
The shorelines of Titan’s hydrocarbon seas trace flooded erosional landforms such as river valleys; however, it isunclear whether coastal erosion has subsequently altered these shorelines. Spacecraft observations and theo-retical models suggest that wind may cause waves to form on Titan’s seas, potentially driving coastal erosion,but the observational evidence of waves is indirect, and the processes affecting shoreline evolution on Titanremain unknown. No widely accepted framework exists for using shoreline morphology to quantitatively dis-cern coastal erosion mechanisms, even on Earth, where the dominant mechanisms are known. We combinelandscape evolution models with measurements of shoreline shape on Earth to characterize how differentcoastal erosion mechanisms affect shoreline morphology. Applying this framework to Titan, we find that theshorelines of Titan’s seas are most consistent with flooded landscapes that subsequently have been eroded bywaves, rather than a uniform erosional process or no coastal erosion, particularly if wave growth saturates atfetch lengths of tens of kilometers.
2. METHODS OF CELL ADHESION
Cells adhere by
cell junctions,
cell adhesion molecules,
or substrate adhesion molecules
3. TYPES OF CELL ATACHMENTS
• Tight junctions are regions where membranes of
adjacent cells actually fuse.
• They encircle the whole cell and provide a barrier for
leakage between cells.
• Desmosomes are spot rivets that weld cells together.
• A cytoplasmic plaque is connected by inter cellular
protein filaments, and the plaques are connected to the
intermediate filaments within the cytoplasm
• Gap junctions are small channels between cells that
allow for intercellular communication
4. CELL ADHESION MOLECULES
Cell adhesion molecules (CAMs) are glycoproteins
with 3 major domains:
The extracellular domain allows one CAM to bind to
another on an adjacent cell. The binding can be to the
same type of cell (homotypic) or to a different cell type
(heterotypic).
The transmembrane domain links the CAM to the
plasma membrane through hydrophobic forces.
The cytoplasmic domain is directly connected to the
cytoskeleton by linker proteins. This anchoring is
important to prevent lateral diffusion of adhesion
molecules in the membrane.
5. Cadherins mediate calcium-
dependent cell adhesion
Cadherins are the most prevalent CAMs in vertebrates. They are rapidly degraded
by proteases in the absence of Ca++. There are 4 major types:
E cadherins in epithelial cells
P cadherins in placenta
N cadherins in neural tissue
L cadherins in liver
Each associates with its own type.
6. SUBSTRATE ADHESION MOLECULES
(SAMS)
• Spaces between cells are filled with ECM that consists of:
• 1. Amorphous ground substance: a gel-like material that absorbs
water.
• 2. Meshwork of fibers that reinforce the ground substance.
• The ECM influences cell migration, cell shape, cell gene expression,
and cell differentiation.
7. GLYCOSAMINOGLYCANS AND PROTEOGLYCANS
FORM THE AMORPHOUS GROUND SUBSTANCE
• Glycosaminoglycan: long unbranched polysaccharide
chains composed of repeating units of disaccharides.
One sugar is an amino sugar (n-acetyl glucosamine) and
the other is a uronic acid
• Proteoglycans: glycosaminoglycans are covalently linked
to core proteins The core proteins have have many side
chains of glycosaminoglycans. They attract Na+ and
water and expand to form gels that occupy space between
cells.
8. INTEGRINS MEDIATE ADHESION TO ECM
• Integrin are a family of transmembrane glycoproteins that are composed of 2
chains, a and b. There are 40 different types of a chains and 8 types of b chains
that can combine to form a large number of different integrin molecules.
• The a chain has binding sites for Ca++ and Mg++ which are needed for
integrins to adhere. The 2 subunits form the site that binds to the RGD domain
on ECM.
9. Two main ways in which animal cells are bound together: in connective tissue, the main
stress-bearing component is the extracellular matrix. In epithelial tissue, it is the
cytoskeletons of the cells themselves linked from cell to cell by anchoring junctions. Cell-
matrix attachments bond epithelial tissue to the connective tissue beneath it.
10. PLANTS CELL ADHESION
The adhesive mechanism at the middle lamella that
maintains cell to cell links until separation is not known.
A calcium-dependent mechanism involving cross links of
homogalacturonan regions of pectic polysaccharides is
probably the most widely considered possibility , although this
does not seem to have been rigorouslytested. The main evidence
for a role for pectins is derived
from observations of the action of pectinases and calcium
chelators resulting in cell separation in certain tissues.
However, the ability of these treatments to result in cell
separation varies between tissues
11. PLANT TISSUES
• The overall structural organization of plants is generally simpler than that of animals. For instance,
plants have only four broad types of cells, which in mature plants form four basic classes of tissue:
dermal tissue interacts with the environment; vascular tissue transports water and dissolved substances
(e.g., sugars, ions); space filling ground tissue constitutes the major sites of metabolism; and
sporogenous tissue forms the reproductive organs
• Plant tissues are organized into just four main organ systems: stems have support and transport
functions; roots provide anchorage and absorb and store nutrients; leaves are the sites of
photosynthesis; and flowers enclose the reproductive structures. Thus at the cell, tissue, and organ
levels, plants are generally less complex than most animals
12. • Unlike animals, plants do not replace or repair old or damaged cells or tissues;
they simply grow new organs.
• Most importantly in contrast with animals, few cells in plants directly contact
one another through molecules incorporated into their plasma membranes
• Instead, plant cells are typically surrounded by a rigid cell wall that contacts
the cell walls of adjacent cells
• Also in contrast with animal cells, a plant cell rarely changes its position in the
organism relative to other cells
• These features of plants and their organization have determined the
distinctive molecular mechanisms by which their cells are incorporated into
tissues
13. INTERCELLULAR SPACE FORMATION
• Intercellular space formation occurs to some extent in all
tissues, generally at cell corners.
• Their formation usuallyinvolves disruption of a highly localized
region of a parent cell wall leading to the connection of a
middle lamella between daughter cells with the middle lamella
of the parent cell.
• It will be of great interest to determine the means of the
regulation of this disruption and the relativeinvolvement of the
participating cells.
14. CELLULAR INTERACTIONS IN PLANTS
• Due to the lack of relative cell movement and active adhesion phenomena
involving recognition and sorting, the cell signalling requirements for plant cells
during development would therefore seem to be distinct fromthose of animal
cells.
• Cell position with a population of meristematic cells appears to be an important
factor in the determination of cell fate .
• A component cell of a multicellular structure needs to know if it has neighbours
and who they are, i.e. what is its own developmental context.
• Whatever the cell signalling mechanisms are, the maintenance of close cell
contact observed within meristems and embryos would seem to be a
requirement of the setting up of the early cell distinctions. The subsequent
development of the cells can be viewed as a gradual relaxation of this tight
adherence in a developmentally regulated manner.