The Beer-Lambert Law describes how the intensity (I) of monochromatic light is attenuated when passing through a solution due to absorption. I decreases exponentially with increasing concentration (c) of the absorbing material, according to Beer's Law, and with increasing path length (l) through the material, according to Lambert's Law. The relationship can be expressed as: A (or extinction, E) = kcl, where k is the material's molar extinction coefficient and c and l have the above definitions. A plot of E versus c at constant l will yield a straight line passing through the origin.
This presentation gives you thorough knowledge about the IR Spectroscopy. This include basic principle, type of vibrations, factors influencing vibrational frequency, instrumentation and applications of IR Spectroscopy. This is the most widely used technique for identifying unknown functional group depending on the vibrational frequency.
This presentation gives you thorough knowledge about the IR Spectroscopy. This include basic principle, type of vibrations, factors influencing vibrational frequency, instrumentation and applications of IR Spectroscopy. This is the most widely used technique for identifying unknown functional group depending on the vibrational frequency.
ION EXCHANGE CHROMATOGRAPHY
By M.Vharshini
B.Sc. Bio Medical Science
Sri Ramachandra University
ION EXCHANGE CHROMATOGRAPHY
Ion-exchange chromatography is a process that allows the separation of ions and polar molecules based on their affinity to the ion exchanger.
It can be used for almost any kind of charged molecule including large proteins, small nucleotides and amino acids.
Cations or Anions can be separated using this method.
PRINCIPLE
It is based on the reversible electrostatic interaction of ions with the separation matrix (i.e.)
The separation occurs by reversible exchange of ions between the ions present in the solution and those present in the ion exchange resin.
CLASSIFICATION OF RESINS
According to the chemical nature they classified as-
1. Strong cation exchange resin
2. Weak cation exchange resin
3. Strong anion exchange resin
4. Weak anion exchange resin
According to the Source they can -
Natural resins : Cation - Zeolytes, Clay
Anion - Dolomite
Synthetic resins: Inorganic & Organic resins
◘Organic resins are polymeric resin matrix.
The resin composed of –
Polystyrene (sites for exchangeable functional groups)
Divinyl benzene(Cross linking agent)-offers stability.
Ion exchange resin should have following requirements
»It must be chemically stable.
»It should be insoluble in common solvents.
» It should have a sufficient degree of cross linking.
»The swollen resin must be denser than water.
»It must contain sufficient no. of ion exchange groups.
Physical properties of ion exchange resins
Cross linking:
It affects swelling & strength & solubility
Swelling:
When resin swells, polymer chain spreads apart
Polar solvents → swelling
Non-polar solvents → contraction
Swelling also affected electrolyte concentration.
Particle size and porosity
Increase in surface area & decrease in particle size will increase the rate of ion exchange.
Regeneration
Cation exchange resin are regenerated by treatment with acid, then washing with water.
Anion exchange resin are regenerated by treatment with NaOH, then washing with water until neutral.
EXPERIMENTAL SETUP OF ION EXCHANGE CHROMATOGRAPHY
Metrohm 850 Ion chromatography system
Instrumentation of ion exchange chromatography
PRACTICAL REQUIREMENTS
1.Column
» glass, stainless steel or polymers
2.Packing the column
» Wet packing method:
A slurry is prepared of the eluent with the stationary phase powder and then carefully poured into the column. Care must be taken to avoid air bubbles.
3.Application of the sample
After packing, sample is added to the top of the stationary phase, use syringe or pipette.
This layer is usually topped with a small layer of sand or with cotton or glass wool to protect the shape of the organic layer from the velocity of newly added eluent.
4.Mobile phase
Acids, alkalis, buffers…
6.Stationary phase
The ionic
It would be use full to All Needy People. It involve information about NMR Spectroscopy ( a spectroscopic techniques), factors influencing , proton NMR and their applications of NMR as well as Nuclear magnetic imaging.
HPLC is a High Performance liquid Chromatography.
High Pressure Liquid Chromatography.
High Priced Liquid Chromatography.
It is column chromatography.
It is Liquid Chromatography.
It is modified from of gas chromatography, it is applicable for both Volatile as well as Non volatile compound.
It can mainly divided by two types 1. Normal phase HPLC 2. Reversed Phase HPLC.
It is having a high resolution and separation capacity.
Spectroscopy is the branch of science dealing the study of interaction of electromagnetic radiation with matter. OR
It is the measurement of electromagnetic radiation (EMR) absorbed or emitted when molecule or ions or atoms of a sample move from one energy state to another energy state.
Spectroscopy is the most powerful tool available for the study of atomic & molecular structure and is used in the analysis of a wide range of samples .
This presentation include the detailed explanation of various parts of a UV-Visible spectrophotometer and two types of UV-Visible spectrophotometers-Single beam and Doube beam. It also include the comparison between single beam and double beam spectrophotometers.
ION EXCHANGE CHROMATOGRAPHY
By M.Vharshini
B.Sc. Bio Medical Science
Sri Ramachandra University
ION EXCHANGE CHROMATOGRAPHY
Ion-exchange chromatography is a process that allows the separation of ions and polar molecules based on their affinity to the ion exchanger.
It can be used for almost any kind of charged molecule including large proteins, small nucleotides and amino acids.
Cations or Anions can be separated using this method.
PRINCIPLE
It is based on the reversible electrostatic interaction of ions with the separation matrix (i.e.)
The separation occurs by reversible exchange of ions between the ions present in the solution and those present in the ion exchange resin.
CLASSIFICATION OF RESINS
According to the chemical nature they classified as-
1. Strong cation exchange resin
2. Weak cation exchange resin
3. Strong anion exchange resin
4. Weak anion exchange resin
According to the Source they can -
Natural resins : Cation - Zeolytes, Clay
Anion - Dolomite
Synthetic resins: Inorganic & Organic resins
◘Organic resins are polymeric resin matrix.
The resin composed of –
Polystyrene (sites for exchangeable functional groups)
Divinyl benzene(Cross linking agent)-offers stability.
Ion exchange resin should have following requirements
»It must be chemically stable.
»It should be insoluble in common solvents.
» It should have a sufficient degree of cross linking.
»The swollen resin must be denser than water.
»It must contain sufficient no. of ion exchange groups.
Physical properties of ion exchange resins
Cross linking:
It affects swelling & strength & solubility
Swelling:
When resin swells, polymer chain spreads apart
Polar solvents → swelling
Non-polar solvents → contraction
Swelling also affected electrolyte concentration.
Particle size and porosity
Increase in surface area & decrease in particle size will increase the rate of ion exchange.
Regeneration
Cation exchange resin are regenerated by treatment with acid, then washing with water.
Anion exchange resin are regenerated by treatment with NaOH, then washing with water until neutral.
EXPERIMENTAL SETUP OF ION EXCHANGE CHROMATOGRAPHY
Metrohm 850 Ion chromatography system
Instrumentation of ion exchange chromatography
PRACTICAL REQUIREMENTS
1.Column
» glass, stainless steel or polymers
2.Packing the column
» Wet packing method:
A slurry is prepared of the eluent with the stationary phase powder and then carefully poured into the column. Care must be taken to avoid air bubbles.
3.Application of the sample
After packing, sample is added to the top of the stationary phase, use syringe or pipette.
This layer is usually topped with a small layer of sand or with cotton or glass wool to protect the shape of the organic layer from the velocity of newly added eluent.
4.Mobile phase
Acids, alkalis, buffers…
6.Stationary phase
The ionic
It would be use full to All Needy People. It involve information about NMR Spectroscopy ( a spectroscopic techniques), factors influencing , proton NMR and their applications of NMR as well as Nuclear magnetic imaging.
HPLC is a High Performance liquid Chromatography.
High Pressure Liquid Chromatography.
High Priced Liquid Chromatography.
It is column chromatography.
It is Liquid Chromatography.
It is modified from of gas chromatography, it is applicable for both Volatile as well as Non volatile compound.
It can mainly divided by two types 1. Normal phase HPLC 2. Reversed Phase HPLC.
It is having a high resolution and separation capacity.
Spectroscopy is the branch of science dealing the study of interaction of electromagnetic radiation with matter. OR
It is the measurement of electromagnetic radiation (EMR) absorbed or emitted when molecule or ions or atoms of a sample move from one energy state to another energy state.
Spectroscopy is the most powerful tool available for the study of atomic & molecular structure and is used in the analysis of a wide range of samples .
This presentation include the detailed explanation of various parts of a UV-Visible spectrophotometer and two types of UV-Visible spectrophotometers-Single beam and Doube beam. It also include the comparison between single beam and double beam spectrophotometers.
A 2000+ slide PowerPoint presentation from www.sciencepowerpoint.com becomes the roadmap for an amazing learning experience. Complete with homework package, built-in activities with directions, built-in quizzes, unit notes, follow along worksheets, answer keys, video links, review games, rubrics, and much more.
Also included are directions on how create a student version of the unit that is much like the teachers but missing the answer keys, quizzes, PowerPoint review games, hidden box challenges, owl, and surprises meant for the classroom. This is a great resource to distribute to your students and support professionals and will only take you a few minutes to create.
This is a great introductory unit that covers science topics associated with Lab Safety, Magnification, Base Units of the Metric System, Scientific Method, Inferences, and Observation Skills (See list below for more topics covered). This unit includes an interactive and engaging PowerPoint Presentation of 2000 slides with built in class notes (Red Slides), lab activities, project ideas, discussion questions, assessments (Quiz Wiz), and challenge questions with answers.
Text is in large print (32 font) and is placed at the top of each slide so it can seen and read from all angles of a classroom. A shade technique, as well as color coded text helps to increase student focus and allows teacher to control pace of the lessons. Also included is a 10 page assessment / bundled homework that chronologically follows the slideshow for nightly homework and end of the unit assessment, as well as a 9 page modified assessment. 14 pages of class notes with images are also included for students who require modifications, as well as answer keys to both of the assessments for support professionals, teachers, and home school parents. Several video links are provided and a slide within the slideshow cues teacher / parent when the videos are most relevant to play. Video shorts usually range from 2-7 minutes. One PowerPoint review game (125+ slides)is included. Answers to the PowerPoint review game are provided in PowerPoint form so students can self-assess. Lastly, several class games such as guess the hidden picture beneath the boxes, and the find the hidden owl somewhere within the slideshow are provided. Difficulty rating of 5 (Ten is most difficult)
Thank you for time and if you have any questions please feel free to contact me at www.sciencepowerpoint@gmail.com. Best wishes.
Teaching Duration = 4+ Weeks
Sincerely,
Ryan Murphy M.Ed
Science PowerPoints
Biochemical mechanismsof acid base balance and acid base disordersrohini sane
A comprehensive presentation on Biochemical Mechanisms of Acid-Base Balance and Acid Base disorders for undergraduate medical, dental, biotechnology and pharmacology students for self –learning. pH maintained in tissues under physiological conditions are mentioned.
Basic concepts of buffers & their types (Acidic buffer & alkaline) are illustrated. Mechanisms of action of Buffer system for acid base balance is explained for perusal of students. Acids produced in a human body during metabolisms are listed.
Front line defense, second line defense (kidney) and dilution factor in regulation of pH in human body for acid-base balance are presented.
Blood buffers involved in acid base balance are classified. Ratio involved, Advantages and Disadvantages of Bicarbonate Buffer / Phosphate buffer system in acid base balance elaborated for their clinical applications.
Comparison of Buffering action of hemoglobin verses plasma proteins is presented under Protein buffer system. Mechanism of Buffering action of plasma proteins in Acidic and alkaline conditions is explained. Working of Hemoglobin buffer system in lung and tissue is illustrated.
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Role Renal Mechanism during acidosis and alkalosis using Bicarbonate mechanism, Phosphate mechanism, Ammonia mechanism, HCO³⁻ reabsorption and NH ₃ production is simplified. pCO₂, Concentration of K⁺ in ICF (intracellular fluid). Plasma Concentration of Cl⁻ ions and Concentration of adrenal-corticoids Hormones as factors affecting bicarbonate re absorption in proximal renal tubular cells are explained in lucid manner.
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Importance of Anion Gap in detection of metabolic acidosis & alkalosis presented. Anion Gap in Metabolic acidosis (acid accumulation and bicarbonate ion loss) is elucidated. Urinary anion gap as indicator of effective renal acid secretion during acidosis is explained diagrammatically. Clinical Conditions associated with increase and decrease in Anion Gap are listed. Comparison of Anion Gap between Metabolic acidosis And Metabolic alkalosis is explained diagrammatically.
Importance of Glutaminase, Glutaminase, L- amino acid oxidase, and Glycine oxidase in Ammonia mechanism in kidney-Distal tubular cells for acid base balance is presented.
Comparison of Definition, Ratio, Biochemical findings in Uncompensated and Compensatory phase of between different types of acidosis & alkalosis is done for their laboratory diagnosis. Clinical conditions associated in different types of acidosis & alkalosis are listed. Google images are used to convey the concept of the subject to self-learners.
Properties of water and aqueous solutionssabir shah
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Photometer, Principle, Beer-Lambert's Laws, Types, Applications, care and mai...ssuser19d1c0
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LAMBERT'S LAW :
when monochromatic radiation is passed through a medium the rate of the decrease in the intensity of radiation with thickness of the medium is directly proportional to intensity of the incident radiation
dI/dt α I
BEER'S LAW
According to this law, when a beam of monochromatic radiation is passed through a solution of absorbing species, the intensity of beam of monochromatic light decreases exponentially with increase in concentration of absorbing species
dI/dc α I
Visible spectroscopy is a technique that uses visible light to study the absorption, transmission, and reflection of light by materials. Visible light is the portion of the electromagnetic spectrum that is visible to the human eye and ranges from about 400 to 800 nanometers in wavelength.
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Visible spectroscopy is widely used in chemistry, biochemistry, physics, and materials science. It is often used to determine the concentration of a colored compound in solution or to monitor the progress of a chemical reaction by measuring the changes in the absorbance of light over time. The technique is also used to study the electronic structure of molecules and solids, as well as to characterize the optical properties of materials such as pigments, dyes, and semiconductors
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I also mentioned about effect of solvent on Absorption shift how the polar and non-polar compound are affected when we change the polarity of solvent.
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2. The Beer –Lambert Law
• When a monochromatic light of initial intensity Io passes
through a solution in a transparent vessel, some of the light
is absorbed so that the intensity of the transmitted light I
is less than Io .There is some loss of light intensity from
scattering by particles in the solution and reflection at the
interfaces, but mainly from absorption by the solution.
• Therelationship between I and Io depends on the path
length of theabsorbing medium, l, and the concentration of
the absorbing solution,c. These factors are related in the
laws of Lambert and Beer
3.
4. Lambert’s law
• When a ray of monochromatic light
passes throughan absorbing medium
its intensity decreases exponentially
as the length of the absorbing
medium increases.
5. Beer’s law :
• When a monochromatic light passes
through an absorbing medium its
intensity decreases exponentially as
the concentration of the absorbing
medium increases.
6.
7. Transmittance:
• The ratio of intensities is known as the
transmittance (T) and this is usually
expressed as percentage
8. Extinction
• If logarithms are taken of the
equation instead of a ratio then
9. • The expression log10 Io/I is known as the
extinction (E) or absorbance(A). The extinction is
some times referred as optical density.
• Therefore
• A (or) E = k cl
• where k is molar extinction co-efficient for the
absorbing material atwave length l, c = molar
concentration of the absorbing solution,l = path
length in the absorbing material in cm.
10. • If the Beer- Lambert law is obeyed
correctly and l is kept constant, then
a plot of extinction against
concentration gives a straight line
passing through the origin
• Extinction, E=log10 Io/I
