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ASSISTED REPRODUCTIVE TECHNIQUES
MALE SEVERE
 OLIGOSPERMIA/AZOOSPERMIA
 ARTIFICAL INSEMINATION HUSBAND

 SEMEN PREPERATION
         NORMAL
         WASH
         CONCENTRATE
         MEDIA GRADIENTS

   INTRA-VAGINAL
   INTRA-CERVICAL
   INTRA-UTERINE
   INTRA-FALLOPIAN TUBE
   DIRECT INTRA-PERITONEAL
Prof. M.C.Bansal
       MBBS,MS,MICOG,FICOG
           Professor OBGY
       Ex-Principal & Controller
  Jhalawar Medical College & Hospital
Mahatma Gandhi Medical College, Jaipur.
ASSISTED REPRODUCTIVE TECHNIQUES
MALE SEVERE OLIGOSPERMIA/AZOOSPERMIA

 MICRO-ASSISTED FERTILISATION TECHNIQUES
          ZONA DRILLING
          SUB-ZONAL INJECTION
          INTRA-CYTOPLASMIC SPERM INJECTION
 EPIDIDYMAL SPERM ASPIRATION
              MESA - MICROSCOPIC
              PESA - PERCUTANEOUS
              TESA - TESTICULAR
        AVAILABILITY of CRYO-PRESERVATION
ASSISTED REPRODUCTIVE TECHNIQUES
 SIFT – SPERM INTRA-FALLOPIAN TRANSFER
 ZIFT – ZYGOTE INTRA-FALLOPIAN TRANSFER
 GIFT – GAMETE INTRA-FALLOPIAN TRANSFER
 IVF & ET –   IN-VITRO FERTILISATION & EMBRYO
                TRANSFER

    CLASSICAL TEST-TUBE BABY
    LOUISE BROWN :Born-1978, Drs. Edwards & Steptoe
ASSISTED REPRODUCTIVE TECHNIQUES
 INDUCTION OF OVULATION – MULTIPLE FOLLICLE
 ULTRASONOGRAPHIC FOLLICULAR STUDY
 ARTIFICIAL LH SURGE – INJ Beta hCG 5000 IU
                        Follicle Size >18 mm
 OVUM RETREIVAL - USG Guidance/Laproscopic
                       Multiple OVA
 IN-VITRO PROCEDURE (FERTILISATION) --
                       USUALLY 3 OVA USED
                       REST CRYOPRESERVED
 TRANSFER PROCEDURE
 ULTRASOUND MONITORING OF DEVELOPING EMBRYOS

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Assisted reproductive techniques

  • 1. ASSISTED REPRODUCTIVE TECHNIQUES MALE SEVERE OLIGOSPERMIA/AZOOSPERMIA  ARTIFICAL INSEMINATION HUSBAND  SEMEN PREPERATION NORMAL WASH CONCENTRATE MEDIA GRADIENTS  INTRA-VAGINAL  INTRA-CERVICAL  INTRA-UTERINE  INTRA-FALLOPIAN TUBE  DIRECT INTRA-PERITONEAL
  • 2. Prof. M.C.Bansal MBBS,MS,MICOG,FICOG Professor OBGY Ex-Principal & Controller Jhalawar Medical College & Hospital Mahatma Gandhi Medical College, Jaipur.
  • 3. ASSISTED REPRODUCTIVE TECHNIQUES MALE SEVERE OLIGOSPERMIA/AZOOSPERMIA  MICRO-ASSISTED FERTILISATION TECHNIQUES ZONA DRILLING SUB-ZONAL INJECTION INTRA-CYTOPLASMIC SPERM INJECTION  EPIDIDYMAL SPERM ASPIRATION MESA - MICROSCOPIC PESA - PERCUTANEOUS TESA - TESTICULAR AVAILABILITY of CRYO-PRESERVATION
  • 4. ASSISTED REPRODUCTIVE TECHNIQUES  SIFT – SPERM INTRA-FALLOPIAN TRANSFER  ZIFT – ZYGOTE INTRA-FALLOPIAN TRANSFER  GIFT – GAMETE INTRA-FALLOPIAN TRANSFER  IVF & ET – IN-VITRO FERTILISATION & EMBRYO TRANSFER CLASSICAL TEST-TUBE BABY LOUISE BROWN :Born-1978, Drs. Edwards & Steptoe
  • 5. ASSISTED REPRODUCTIVE TECHNIQUES  INDUCTION OF OVULATION – MULTIPLE FOLLICLE  ULTRASONOGRAPHIC FOLLICULAR STUDY  ARTIFICIAL LH SURGE – INJ Beta hCG 5000 IU Follicle Size >18 mm  OVUM RETREIVAL - USG Guidance/Laproscopic Multiple OVA  IN-VITRO PROCEDURE (FERTILISATION) -- USUALLY 3 OVA USED REST CRYOPRESERVED  TRANSFER PROCEDURE  ULTRASOUND MONITORING OF DEVELOPING EMBRYOS