The document discusses several assisted reproductive techniques for treating male severe oligospermia or azoospermia including artificial insemination using processed semen, micro-assisted fertilization techniques like ICSI, and epididymal sperm aspiration. It also covers techniques like IVF where eggs are retrieved, fertilized in vitro, and embryos are transferred, with the first "test-tube baby" born in 1978.

1. ASSISTED REPRODUCTIVE TECHNIQUES
MALE SEVERE
OLIGOSPERMIA/AZOOSPERMIA
 ARTIFICAL INSEMINATION HUSBAND
 SEMEN PREPERATION
NORMAL
WASH
CONCENTRATE
MEDIA GRADIENTS
 INTRA-VAGINAL
 INTRA-CERVICAL
 INTRA-UTERINE
 INTRA-FALLOPIAN TUBE
 DIRECT INTRA-PERITONEAL

2. Prof. M.C.Bansal
MBBS,MS,MICOG,FICOG
Professor OBGY
Ex-Principal & Controller
Jhalawar Medical College & Hospital
Mahatma Gandhi Medical College, Jaipur.

3. ASSISTED REPRODUCTIVE TECHNIQUES
MALE SEVERE OLIGOSPERMIA/AZOOSPERMIA
 MICRO-ASSISTED FERTILISATION TECHNIQUES
ZONA DRILLING
SUB-ZONAL INJECTION
INTRA-CYTOPLASMIC SPERM INJECTION
 EPIDIDYMAL SPERM ASPIRATION
MESA - MICROSCOPIC
PESA - PERCUTANEOUS
TESA - TESTICULAR
AVAILABILITY of CRYO-PRESERVATION

4. ASSISTED REPRODUCTIVE TECHNIQUES
 SIFT – SPERM INTRA-FALLOPIAN TRANSFER
 ZIFT – ZYGOTE INTRA-FALLOPIAN TRANSFER
 GIFT – GAMETE INTRA-FALLOPIAN TRANSFER
 IVF & ET – IN-VITRO FERTILISATION & EMBRYO
TRANSFER
CLASSICAL TEST-TUBE BABY
LOUISE BROWN :Born-1978, Drs. Edwards & Steptoe

5. ASSISTED REPRODUCTIVE TECHNIQUES
 INDUCTION OF OVULATION – MULTIPLE FOLLICLE
 ULTRASONOGRAPHIC FOLLICULAR STUDY
 ARTIFICIAL LH SURGE – INJ Beta hCG 5000 IU
Follicle Size >18 mm
 OVUM RETREIVAL - USG Guidance/Laproscopic
Multiple OVA
 IN-VITRO PROCEDURE (FERTILISATION) --
USUALLY 3 OVA USED
REST CRYOPRESERVED
 TRANSFER PROCEDURE
 ULTRASOUND MONITORING OF DEVELOPING EMBRYOS