This document discusses adenoviral cloning vectors. It begins by defining a cloning vector as a small piece of DNA that can be stably maintained in an organism and have foreign DNA inserted into it for cloning purposes. It then discusses viral vectors, noting that they are commonly used to deliver genetic material into cells through transduction. The document focuses on properties of viral vectors, specifically safety features and targeting abilities. It provides details on adenoviruses, noting they can efficiently transfer genes, their structure, applications in gene therapy and vaccination, and their DNA genome capacity. Adeno-associated viruses are also mentioned as attractive for gene therapy due to mild immune response.
Recombinant baculoviruses are widely used to
express heterologous genes in cultured insect cells
and insect larvae. For large-scale applications, the
baculovirus expression vector system (BEVS) is particularly
advantageous.
Recombinant baculoviruses are widely used to
express heterologous genes in cultured insect cells
and insect larvae. For large-scale applications, the
baculovirus expression vector system (BEVS) is particularly
advantageous.
Recombinant viral vectors are genetic engineering tools commonly used for gene transfer purpose with high transfection efficiency and site specific gene insertion.
This presentation covers a general introduction to expression vector, its components, types, and its application. Then it covers some of the expression system with examples.
Cell culture based vaccine??
Cell cultures involve growing cells in a culture dish, often with a supportive growth medium. A primary cell culture consists of cells taken directly from living tissue, and may contain multiple types of cells such as fibroblasts, epithelial, and endothelial cells.
In the United States, 10 different vaccines for chicken pox, hepatitis A, polio, rabies, and rubella are cultured on aborted tissue from two fetal cell lines known as WI-38 and MRC-5. These vaccines are chicken pox, hep-A, hep-A, hep-A/hep-B, polio, rabies, rubella, measles/rubella, mumps/rubella, and MMR II (measles/mumps/rubella).
SYNTHETIC PEPTIDE VACCINES AND RECOMBINANT ANTIGEN VACCINED.R. Chandravanshi
What is a Vaccine?
A vaccine is a substance that is introduced into the body to prevent infection or to control disease due to a certain pathogen (a disease-causing organism, such as a virus, bacteria or parasite). The vaccine “teaches” the body how to defend itself against the pathogen by creating an immune response.
1 Unlike traditional pharmaceuticals, vaccines are biologics since they are made from living organisms (biological sources).
2 Specifically, vaccines are preparations of components derived from (or related to) a pathogen; they can typically induce a protective effect through one to three very small doses, in the range of micrograms to milligrams.
3 Immunity lasts for an extended period, from one year up to lifetime protection, including prevention of disease and/or related sequelae.
Synthetic peptide vaccines represent fragments of protein antigen sequences, synthesizing specific B cell and T cell epitopes offer the potential to induce diseases neutralizing immuno response with completely synthetic structure. Now it is well established that short chain peptides can be used to mimic antigenic sites of viruses and thus can be used the basics for vaccines and development. therefore, attempts have been made to synthesize such peptides which act as the serrogate immuunogens, as an alternative to the existing conventional vaccines.
What are an expression vector? Detailed description of plant gene structure. Plant expression vector systems are generally consists of Ri and Ti plasmids.
The other vectors which are generally used are DNA and RNA viruses.
Topics included - Introduction; explanation; examples like blue white screening method, antibiotic resistance; Extra information regarding - detection of oncogene in vertebrates and role of sleeping beauty; Merits and demerits of insertional inactivation.
Recombinant viral vectors are genetic engineering tools commonly used for gene transfer purpose with high transfection efficiency and site specific gene insertion.
This presentation covers a general introduction to expression vector, its components, types, and its application. Then it covers some of the expression system with examples.
Cell culture based vaccine??
Cell cultures involve growing cells in a culture dish, often with a supportive growth medium. A primary cell culture consists of cells taken directly from living tissue, and may contain multiple types of cells such as fibroblasts, epithelial, and endothelial cells.
In the United States, 10 different vaccines for chicken pox, hepatitis A, polio, rabies, and rubella are cultured on aborted tissue from two fetal cell lines known as WI-38 and MRC-5. These vaccines are chicken pox, hep-A, hep-A, hep-A/hep-B, polio, rabies, rubella, measles/rubella, mumps/rubella, and MMR II (measles/mumps/rubella).
SYNTHETIC PEPTIDE VACCINES AND RECOMBINANT ANTIGEN VACCINED.R. Chandravanshi
What is a Vaccine?
A vaccine is a substance that is introduced into the body to prevent infection or to control disease due to a certain pathogen (a disease-causing organism, such as a virus, bacteria or parasite). The vaccine “teaches” the body how to defend itself against the pathogen by creating an immune response.
1 Unlike traditional pharmaceuticals, vaccines are biologics since they are made from living organisms (biological sources).
2 Specifically, vaccines are preparations of components derived from (or related to) a pathogen; they can typically induce a protective effect through one to three very small doses, in the range of micrograms to milligrams.
3 Immunity lasts for an extended period, from one year up to lifetime protection, including prevention of disease and/or related sequelae.
Synthetic peptide vaccines represent fragments of protein antigen sequences, synthesizing specific B cell and T cell epitopes offer the potential to induce diseases neutralizing immuno response with completely synthetic structure. Now it is well established that short chain peptides can be used to mimic antigenic sites of viruses and thus can be used the basics for vaccines and development. therefore, attempts have been made to synthesize such peptides which act as the serrogate immuunogens, as an alternative to the existing conventional vaccines.
What are an expression vector? Detailed description of plant gene structure. Plant expression vector systems are generally consists of Ri and Ti plasmids.
The other vectors which are generally used are DNA and RNA viruses.
Topics included - Introduction; explanation; examples like blue white screening method, antibiotic resistance; Extra information regarding - detection of oncogene in vertebrates and role of sleeping beauty; Merits and demerits of insertional inactivation.
|| जय माता दी ||
ममतामयी श्री राधे गुरु माँ जी के पावन जनमोहत्सव के उपलक्ष मैं
माँ भगवती की विशाल चौकी का आयोजन किया गया है |
जिस मैं देश भर से पधारे सुप्रसिद्ध भजन गायक,
अपने सुमधुर स्वरों से माँ भगवती का गुणगान करेंगे |
दिनांक : 3 मार्च 2017 शाम 6 बजे से प्रभु इच्छा तक
स्थान :जापानी पार्क ,सेक्टर - 10,रोहिणी ,दिल्ली -85
सम्पर्क करें :टल्ली बाबाजी: 9820969020
|| जय माता दी ||
ममतामयी श्री राधे गुरु माँ जी के पावन जनमोहत्सव के उपलक्ष मैं
माँ भगवती की विशाल चौकी का आयोजन किया गया है |
जिस मैं देश भर से पधारे सुप्रसिद्ध भजन गायक,
अपने सुमधुर स्वरों से माँ भगवती का गुणगान करेंगे |
दिनांक : 3 मार्च 2017 शाम 6 बजे से प्रभु इच्छा तक
स्थान :जापानी पार्क ,सेक्टर - 10,रोहिणी ,दिल्ली -85
सम्पर्क करें :टल्ली बाबाजी: 9820969020
|| जय माता दी ||
ममतामयी श्री राधे गुरु माँ जी के पावन जनमोहत्सव के उपलक्ष मैं
माँ भगवती की विशाल चौकी का आयोजन किया गया है |
जिस मैं देश भर से पधारे सुप्रसिद्ध भजन गायक,
अपने सुमधुर स्वरों से माँ भगवती का गुणगान करेंगे |
दिनांक : 3 मार्च 2017 शाम 6 बजे से प्रभु इच्छा तक
स्थान :जापानी पार्क ,सेक्टर - 10,रोहिणी ,दिल्ली -85
सम्पर्क करें :टल्ली बाबाजी: 9820969020
|| जय माता दी ||
ममतामयी श्री राधे गुरु माँ जी के पावन जनमोहत्सव के उपलक्ष मैं
माँ भगवती की विशाल चौकी का आयोजन किया गया है |
जिस मैं देश भर से पधारे सुप्रसिद्ध भजन गायक,
अपने सुमधुर स्वरों से माँ भगवती का गुणगान करेंगे |
दिनांक : 3 मार्च 2017 शाम 6 बजे से प्रभु इच्छा तक
स्थान :जापानी पार्क ,सेक्टर - 10,रोहिणी ,दिल्ली -85
सम्पर्क करें :टल्ली बाबाजी: 9820969020
The Complexities of Creating for the Kids Category | Robby YungJessica Tams
Delivered at Casual Connect Europe 2017. The kids category is the nexus of some of the most diverse content and business models in the app store. From premium to freemium and subscription services, apps for kids have to be so much more than just fun, and navigating your way to success is all about the rules.
Simposio Desafios en infecciones del trópico Septiembre de 2015
Innovación en salud eje fundamental para un nuevo modelo de salud y su rol en el tratamiento de enfermedades infecciosas.
Objectives:
After the end of the presentation we’ll know -
What is cloning vector?
Why cloning vector?
History
Features of a cloning vector
Types of cloning vector
Plasmid
Bacteriophage
Cosmid
Bacterial Artificial Chromosome (BAC)
Yeast Artificial Chromosome (BAC)
Human Artificial Chromosome (HAC)
Retroviral Vectors
What determines choice of vector?
Vector in molecular gene cloning
Cloning vector - The molecular analysis of DNA has been made possible by the cloning of DNA. The two molecules that are required for cloning are the DNA to be cloned and a cloning vector.
A cloning vector is a small piece of DNA taken from a virus, a plasmid or the cell of a higher organism, that can be stably maintained in an organism and into which a foreign DNA fragment can be inserted for cloning purposes.
Most vectors are genetically engineered.
The cloning vector is chosen according to the size and type of DNA to be cloned.
The vector therefore contains features that allow for the convenient insertion or removal of DNA fragment in or out of the vector, for example by treating the vector and the foreign DNA with a restriction enzyme and then ligating the fragments together.
After a DNA fragment has been cloned into a cloning vector, it may be further subcloned into another vector designed for more specific use.
Washing is the process & technology which is used to modify the appearance, outlook comfort ability and fashion of the garments is called garment washing.
On January 25, 2022, Nature published an article listing seven technologies worthy of attention this year. Targeted genetic therapies was on the list. The remaining six technologies are: Fully finished genomes, Protein structure solutions, Quantum simulation, Precise genome manipulation, Spatial multi-omics), CRISPR-based diagnostics.
Creative Biogene provides world-class packaging services for a variety of viral types using our QVirusTM platform. Creative Biogene's state-of-the-art facilities and highly experienced staff are available in order to assist in all areas of virus vector design and construction, as well as the generation of the virus in a quick turnaround. Our custom viral services are highlighted by a consistent high titer and large delivery viral counts.
Adenovirus, a DNA virus, was first isolated in the 1950s in adenoid tissue-derived cell cultures, hence the name. A large number of acute respiratory, gastrointestinal and eye infections in humans are caused by adenoviruses.
https://www.creative-biogene.com/Support/Virus.html
There is no question that the adenovirus is the most effective means of delivering genes in vivo and in vitro. However, construction of adenovirus vectors and virus packaging is time-consuming and labor-intensive. Creative Biogene is a biotechnology company specializing in custom adenovirus production service. Our state-of-the-art facilities and highly experienced staff are available in order to assist in all areas of adenovirus vector design and construction, as well as the generation of the adenovirus in high titer for use in research.
The increased availability of biomedical data, particularly in the public domain, offers the opportunity to better understand human health and to develop effective therapeutics for a wide range of unmet medical needs. However, data scientists remain stymied by the fact that data remain hard to find and to productively reuse because data and their metadata i) are wholly inaccessible, ii) are in non-standard or incompatible representations, iii) do not conform to community standards, and iv) have unclear or highly restricted terms and conditions that preclude legitimate reuse. These limitations require a rethink on data can be made machine and AI-ready - the key motivation behind the FAIR Guiding Principles. Concurrently, while recent efforts have explored the use of deep learning to fuse disparate data into predictive models for a wide range of biomedical applications, these models often fail even when the correct answer is already known, and fail to explain individual predictions in terms that data scientists can appreciate. These limitations suggest that new methods to produce practical artificial intelligence are still needed.
In this talk, I will discuss our work in (1) building an integrative knowledge infrastructure to prepare FAIR and "AI-ready" data and services along with (2) neurosymbolic AI methods to improve the quality of predictions and to generate plausible explanations. Attention is given to standards, platforms, and methods to wrangle knowledge into simple, but effective semantic and latent representations, and to make these available into standards-compliant and discoverable interfaces that can be used in model building, validation, and explanation. Our work, and those of others in the field, creates a baseline for building trustworthy and easy to deploy AI models in biomedicine.
Bio
Dr. Michel Dumontier is the Distinguished Professor of Data Science at Maastricht University, founder and executive director of the Institute of Data Science, and co-founder of the FAIR (Findable, Accessible, Interoperable and Reusable) data principles. His research explores socio-technological approaches for responsible discovery science, which includes collaborative multi-modal knowledge graphs, privacy-preserving distributed data mining, and AI methods for drug discovery and personalized medicine. His work is supported through the Dutch National Research Agenda, the Netherlands Organisation for Scientific Research, Horizon Europe, the European Open Science Cloud, the US National Institutes of Health, and a Marie-Curie Innovative Training Network. He is the editor-in-chief for the journal Data Science and is internationally recognized for his contributions in bioinformatics, biomedical informatics, and semantic technologies including ontologies and linked data.
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
THE IMPORTANCE OF MARTIAN ATMOSPHERE SAMPLE RETURN.Sérgio Sacani
The return of a sample of near-surface atmosphere from Mars would facilitate answers to several first-order science questions surrounding the formation and evolution of the planet. One of the important aspects of terrestrial planet formation in general is the role that primary atmospheres played in influencing the chemistry and structure of the planets and their antecedents. Studies of the martian atmosphere can be used to investigate the role of a primary atmosphere in its history. Atmosphere samples would also inform our understanding of the near-surface chemistry of the planet, and ultimately the prospects for life. High-precision isotopic analyses of constituent gases are needed to address these questions, requiring that the analyses are made on returned samples rather than in situ.
2. Cloning vector
A cloning vector is a small piece of DNA taken from a virus, a plasmid, or
the cell of a higher organism, that can be stably maintained in an
organism and into which a foreign DNA fragment can be inserted for
cloning purposes.
The vector therefore contains features that allow for the convenient
insertion or removal of DNA fragment in or out of the vector.
For example by treating the vector and the foreign DNA with a restriction
enzyme that cuts the DNA. DNA fragments thus generated contain either
blunt ends or sticky ends.
3. Viral Vectors
Viral vectors are tools commonly used to deliver genetic material into
cells.
This process can be performed inside a living organism or in cell culture.
Delivery of genes by a virus is termed transduction and the infected cells
are described as transduced.
4. Properties of a viral vector
Safety: Viral vectors are created from pathogenic viruses, they are
modified in such a way as to minimize the risk of handling them.
This usually involves the deletion of a part of the viral genome critical for
viral replication.
Such a virus can efficiently infect cells but once the infection has taken
place, requires a helper virus to provide the missing proteins for
production of new virions.
5. Properties of a viral vector
Low toxicity: The viral vector should have a minimal effect on the physiology
of the cell it infects.
Cell type specificity: Most viral vectors are engineered to infect as wide a
range of cell types as possible. The viral receptor can be modified to target
the virus to a specific kind of cell.
Identification: Viral vectors are often given certain genes that help
identify which cells took up the viral genes. These genes are called
Markers. A common marker is antibiotic resistance to a certain antibiotic.
6. Adenoviruses
Adenoviruses can be converted into efficient gene transfer vehicles.
Adenoviral vectors are not inherently dangerous.
The dose of vector delivered is related to the toxicity observed.
7. Adenoviruses
Icosahedral shape.
Composed of 13% DNA & 87% Protein.
Nonenveloped viruses with an icosahedral nucleocapsid containing a
double stranded DNA genome.
Size 90-100 nm.
8. Adenoviruses
Since humans commonly come in contact with adenoviruses which cause
respiratory, gastrointestinal and eye infections, majority of patients have
already developed neutralizing antibodies which can inactivate the virus
before it can reach the target cell.
To overcome this problem scientists are currently investigating
adenoviruses that infect different species to which humans do not have
immunity.
Their primary applications are in gene therapy and vaccination.
9. Adenoviral genome
The adenovirus genome is a linear, 36 Kb double-stranded DNA (dsDNA)
molecule.
It can take up 8kb foreign DNA.
36 kb
10.
11. Adeno-associated viruses
Adeno-associated virus (AAV) is a small virus that infects humans and
some other primate species.
AAV can infect both dividing and non-dividing cells and may incorporate
its genome into that of the host cell.
AAV is not currently known to cause disease and consequently the virus
causes a very mild immune response.
These features make AAV a very attractive candidate for creating viral
vectors for gene therapy.
12. Applications
Basic research
Viral vectors were originally developed as an alternative to transfection of
naked DNA for molecular genetics experiments.
Protein coding genes can be expressed using viral vectors commonly to
study the function of the particular protein.
Viral vectors, especially retroviruses, stably expressing marker genes are
widely used to permanently label cells to track them and their progeny.
13. Gene therapy
Gene therapy is a technique for correcting defective genes responsible
disease development. In the future, gene therapy may provide a way to
cure genetic disorders.
Diseases result from mutations in the DNA sequence for specific genes,
gene therapy trials have used viruses to deliver unmutated copies of
genes to the cells of the patient's body.
14. Vaccines
Viruses expressing pathogen proteins are currently being developed as
vaccines against these pathogens, based on the same as DNA vaccines.
However, since viral vaccines contain only a small fraction of pathogen
genes, they are much safer and infection by the pathogen is impossible.