Nanobiosensors use biological components on the nano-scale to detect target molecules. They consist of a bioreceptor element for molecular recognition connected to a transducer that converts the biological response into a measurable signal. Common transduction methods include optical, electrochemical and mechanical. Examples are nanowire field effect sensors that detect binding as a change in electrical conductivity, and cantilever sensors that detect binding as a change in resonant frequency through surface stress. Nanobiosensors show potential for applications in healthcare diagnostics, environmental monitoring, and more.
The following presentation is only for quick reference. I would advise you to read the theoretical aspects of the respective topic and then use this presentation for your last minute revision. I hope it helps you..!!
Mayur D. Chauhan
A part of nanotechnology. Nanosensors is very hot topic for research. As nanosensor has immense applications in the fields like medical, analysis, research etc. Nanosensor recude the cost and also the time require for analysis.
Nanoelectronics refer to the use of nanotechnology in electronic components. The term covers a diverse set of devices and materials, with the common characteristic that they are so small that inter-atomic interactions and quantum mechanical properties need to be studied extensively.
Surface Plasmon Resonance (SPR) and its ApplicationDr. Barkha Gupta
DR. BARKHA GUPTA
ASSISTANT PROFESSOR (VETERINARY BIOCHEMISTRY)
DEPARTMENT OF VETERINARY PHYSIOLOGY AND BIOCHEMISTRY
POST GRADUATE INSTITUTE OF VETERINARY EDUCATION AND RESEARCH (PGIVER), JAIPUR RAJASTHAN UNIVERSITY OF VETERINARY AND ANIMAL SCIENCES (RAJUVAS), BIKANER
YouTube Channel: Barkha’s Vet Sphere
here you can find the most rare topics in detail
all fields of chemistry are deeply understood here for presenting the lectures
stay blessed and keep supporting
This presentation will explain to you the types of nanosensors in different fields and application of nanosensors in detail which will enhance your knowledge in the field of nanotechnology
The revolution of nanotechnology in molecular biology gives an opportunity to detect and manipulate atoms and molecules at the molecular and cellular level.
intoduction to lumiscence
introduction and principle of chemilumiscence
different types of lumiscence
detail of the electrochemilumiscence, working, principle, instrumentation, measurin.
application in medical field
difference between chemilumiscence and elecrochemiluminescence
The following presentation is only for quick reference. I would advise you to read the theoretical aspects of the respective topic and then use this presentation for your last minute revision. I hope it helps you..!!
Mayur D. Chauhan
A part of nanotechnology. Nanosensors is very hot topic for research. As nanosensor has immense applications in the fields like medical, analysis, research etc. Nanosensor recude the cost and also the time require for analysis.
Nanoelectronics refer to the use of nanotechnology in electronic components. The term covers a diverse set of devices and materials, with the common characteristic that they are so small that inter-atomic interactions and quantum mechanical properties need to be studied extensively.
Surface Plasmon Resonance (SPR) and its ApplicationDr. Barkha Gupta
DR. BARKHA GUPTA
ASSISTANT PROFESSOR (VETERINARY BIOCHEMISTRY)
DEPARTMENT OF VETERINARY PHYSIOLOGY AND BIOCHEMISTRY
POST GRADUATE INSTITUTE OF VETERINARY EDUCATION AND RESEARCH (PGIVER), JAIPUR RAJASTHAN UNIVERSITY OF VETERINARY AND ANIMAL SCIENCES (RAJUVAS), BIKANER
YouTube Channel: Barkha’s Vet Sphere
here you can find the most rare topics in detail
all fields of chemistry are deeply understood here for presenting the lectures
stay blessed and keep supporting
This presentation will explain to you the types of nanosensors in different fields and application of nanosensors in detail which will enhance your knowledge in the field of nanotechnology
The revolution of nanotechnology in molecular biology gives an opportunity to detect and manipulate atoms and molecules at the molecular and cellular level.
intoduction to lumiscence
introduction and principle of chemilumiscence
different types of lumiscence
detail of the electrochemilumiscence, working, principle, instrumentation, measurin.
application in medical field
difference between chemilumiscence and elecrochemiluminescence
Historically architecture has likened the city as an organism and looked to nature for design inspiration. Until recently the tools that have enabled architects to engage with what R. Buckminster Fuller called the ‘drivers of biology’, have not been available and architects use biological systems in a symbolic way called biological ‘formalism’ where aesthetics are prioritized over function. Recent developments in Synthetic Biology, which were demonstrated at Artificial Life XI suggested it was possible to design and engineer materials that meet the requirements necessary for a new generation of smart materials.
Diaporama ProgrèS Technique Et Croissance 2007 2008guestf961ba
Ce diaporama présentant les liens entre croissance et déprogrès technique reprend :
1 - le diaporama de T Larribe sur robinson (remarquable comme toujours)
2 - il s'appuie sur des éléments à une diaporama d'HEC
3 Il emprunte des animations à mr Rodriguez
Pression économique sur les coûts, vitesse accélérée, nouvelles frontières, fournisseurs moins pertinents… il semble urgent de reconsidérer les activités de la DSI et de faire évoluer son modèle !
Le portrait robot du CDO d'une entreprise françaiseEY
Frédéric Levaux, Chief Digital Officer d'EY en France, intervenait le 26 novembre 2015 lors du CDO Day organisé par L'Usine Digitale sur le thème "L’agenda du CDO : quelles priorités, quelles échéances et quelles ambitions".
Presentación del evento #BIGTOURISM #WTC2014 @CIBBVA @SEGITTUR en BBVA Innovation Center, el día 13 de Noviembre 2014.
Su Streaming lo puedes ver en: https://www.centrodeinnovacionbbva.com/eventos/evento-big-tourism-we-are-all-tourists-1ajornada
Biosensor is the Talk of The Day. It made possible, the conversion of yesteryear's cumbersome experiments to an easier, faster all the while improving its sensitivity and specificity. This article will help you to gain an acquaintance about it, its properties, etc.
Dr.S.Karthikumar
Assistant Professor, Dept. of Biotechnology
Kamaraj College of Engineering and Technology
Madurai, Tamilnadu, INDIA
skarthikumar@gmail.com
Dr.S.Karthikumar
Asst. Prof., Dept. of Biotechnology
Kamaraj College of Engineering and Technology
S.P.G.C.Nagar, Virudhunagar, Tamilnadu, India
skarthikumar@gmail.com
Biosensor and its Applications.
Biosensors are analytical devices that combine a biological component with a physicochemical detector to provide specific and sensitive detection of target analytes.
Importance: Biosensors have revolutionized the way we detect and monitor various substances, from biomarkers to environmental pollutants.
Biosensor is an leading Biological technology now. It is an application of Biotechnology. It makes laboratory tests more fast and easy to carry out. It is cost effective, more accurate precise, and have less errors.
Biosensors: General Principles and ApplicationsBhatt Eshfaq
A biosensor is an analytical device, used for the detection of a chemical substance, that combines a biological component with a physicochemical detector.
Esiti esame Bioch Siste Umana del 23.01.2017.
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Proposte stage 2016-2017. In verde: studenti e relativi periodi GIA' ASSEGNATI.
In giallo: studenti e periodi disponibili ANCORA DA CONFERMARE.
I gialli dovrebbero farmi sapere (VIA MAIL) la loro decisione al più presto per eventuale liberazione di posti. grazie.
2. BIOSENSORS
• A device incorporating a biological sensing
element either intimately connected to or
integrated within a transducer.
• Recognition based on affinity between
complementary structures like:
enzyme-substrate, antibody-antigen , receptor-
hormone complex.
• Selectivity and specificity depend on
biological recognition systems connected to
a suitable transducer.
3.
4.
5.
6. Biosensor Development
• 1916 First report on the immobilization of proteins: adsorption of
invertase on activated charcoal.
• 1956 Invention of the first oxygen electrode [Leland Clark]
• 1962 First description of a biosensor: an amperometric enzyme electrode
for glucose. [Leland Clark, New York Academy of Sciences Symposium]
• 1969 First potentiometric biosensor: urease immobilized on an ammonia
electrode to detect urea. [Guilbault and Montalvo]
• 1970 Invention of the Ion-Selective Field-Effect Transistor (ISFET).
• 1972/5 First commercial biosensor: Yellow Springs Instruments glucose
biosensor.
• 1976 First bedside artificial pancreas [Clemens et al.]
• 1980 First fiber optic pH sensor for in vivo blood gases.
• 1982 First fiber optic-based biosensor for glucose
• 1983 First surface plasmon resonance (SPR) immunosensor.
• 1987 Launch of the blood glucose biosensor[ MediSense]
11. Electrical sensing
Nano-bio interfacing how to translate the
biological information onto electrical signal ?
• Electrochemical
– Redox reactions
• Electrical
– FET
(Nano wires; Conducting Electro-active Polymers)
12. Electrical sensors
FET based methods – FET – Field Effect Transistors
ISFET – Ion Sensitive FET
CHEMFET – Chemically Sensitive FET
SAM-FET – Self Assembly Monolayer Based FET
K Corrente elettrica
13. Nanowires Biosensors
Field Effect
Nanobiosensors (FET)
• Functionalize the nano-wires
• Binding to bio-molecules will affect the nano-wires conductivity.
14. Nanowire Field Effect Nanobiosensors (FET)
Sensing Element
Semiconductor channel (nanowire) of the
transistor.
• The semiconductor channel is fabricated using nanomaterials such
carbon nanotubes, metal oxide nanowires or Si nanowires.
• Very high surface to volume radio and very large portion of the atoms are
located on the surface. Extremely sensitive to environment
20. NW grow across gap between electrodes
Growing NW connect to the second electrode
21. Form trench in Si and NW grows NW connects to opposite
Deposit catalyst perpendicular sidewall
22.
23.
24. Schematic shows two
nanowire devices, 1 and 2,
where the nanowires are
modified with different
antibody receptors.
Specific binding of a single
virus to the receptors on
nanowire 2 produces a
conductance change
(Right) characteristic of the
surface charge of the virus
only in nanowire 2. When
the virus unbinds from the
surface the conductance
returns to the baseline
value.
37. Bio-molecule sensing
Detection of biomolecules by
simple mechanical transduction:
target molecule
- cantilever surface is covered
receptor molecule by receptor layer
gold (functionalization)
SiNx cantilever
- biomolecular interaction
between receptor and
target molecules
(molecular recognition)
target binding
deflection d - interaction between adsorbed
molecules induces surface stress
change
bending of cantilever
38.
39. resonance frequency mass-sensitive detector
A
1 k
f1
2 m eff f1
f1 f
A
1 k m
f2
2 m eff m f2
f2 f
A mass sensitive resonator transforms an additional mass loading
into a resonance frequency shift mass sensor
B. Kim et al, Institut für Angewandte Physik - Universität Tübingen
40. Magnetic sensing
• magnetic fields to sense magnetic nano-
particles that have been attached to biological
molecules.
41. Stabilization of Magnetic Particles with various Streptavidin Conc.
Ligand-functionalized Magnetic nano -
particle
Analyte
10 min reaction
None 1 ng/ml 100 ng/ml 100 g/ml
Cleaning
5 min deposition
Anti-analyte Antibody T. Osaka, 2006
42. Magnetic sensing
Sensing plate
Magnetic head
Activated pixel
Idle pixel
Magnetic field sensor
Magnetic sensing