1. Chromatography

2. Chromatography
Definition:
“A technique by which we can separate components of a mixture due
to their different distribution between stationary and mobile phase is
called Chromatography”
Chromatography, literally meaning "color writing"

3. History of Chromatography
• It was first employed by Russian scientist Mikhail Tsvet in 1900
• It was primarily for the separation of plant pigments such
as chlorophyll, carotenes, and xanthophyll
• New types of chromatography developed during the 1930s and
1940s made the technique useful for many separation processes
• Chromatography technique developed substantially as a result of
the work of Archer John Porter Martin and Richard Laurence
Millington Synge during the 1940s and 1950s
• Advances are continually improving the technical performance of
chromatography

4. Components
Components of Chromatography:
 Mixture containing Analyte
 Stationary Phase
 Mobile Phase

5. Principle of Chromatography
A component which is quite soluble in the stationary phase will take
longer to travel through it than a component which is not very soluble
in the stationary phase but very soluble in the mobile phase

6. Types of Chromatography
Adsorption Chromatography
It utilizes a mobile liquid or gaseous phase that is adsorbed onto the
surface of a stationary solid phase
Partition Chromatography
This form of chromatography is based on a thin film formed on the
surface of a solid support by a liquid stationary phase. Solute
equilibrates between the mobile phase and the stationary liquid

7. Uses of Chromatography
 It can be used in crime scene investigations
 In hospitals it can be used to detect alcohol levels in a patient's
blood stream

It can be used for environmental agencies to determine the level of
pollutants in water supplies

It can be used to purify chemicals needed to make a product in a
manufacturing plant

It can be used by pharmacists to determine the amount of each
chemical found in each product