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High performance liquid chromatography

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High performance liquid chromatography

  1. 1. HIGH PERFORMANCE LIQUID CHROMATOGRAPHY 1
  2. 2. CONTENTS: The components of the high performance liquid chromatograph (HPLC). The separation process. The chromatogram. 2
  3. 3. Component: Solvent Pumps Column Detector Chromatogram 3
  4. 4. 4 Separation in based upon differential migration between the stationary and mobile phases. Stationary Phase - the phase which remains fixed in the column, e.g. C18, Silica Mobile Phase - carries the sample through the stationary phase as it moves through the column. Injector Detector Column Solvents Mixer Pumps High Performance Liquid Chromatograph Waste
  5. 5. 5 Injector Detector Column Solvents Mixer Pumps Chromatogram Start Injection mAU time High Performance Liquid Chromatograph
  6. 6. 6 Injector Detector Column Solvents Mixer Pumps Chromatogram Start Injection mAU time
  7. 7. 7 Injector Detector Column Solvents Pumps Mixer Chromatogram Start Injection mAU time
  8. 8. 8 Injector Detector Column Solvents Pumps Mixer Chromatogram Start Injection mAU time
  9. 9. 9 Injector Detector Column Solvents Pumps Mixer Chromatogram Start Injection mAU time
  10. 10. 10 Injector Detector Column Solvents Pumps Mixer Chromatogram Start Injection mAU time
  11. 11. 11 Injector Detector Column Solvents Pumps Mixer Chromatogram Start Injection mAU time
  12. 12. 12 Injector Detector Column Solvents Pumps Mixer Chromatogram Start Injection mAU time
  13. 13. 13 Injector Detector Column Solvents Pumps Mixer Chromatogram Start Injection mAU time
  14. 14. 14 Injector Detector Column Solvents Pumps Mixer Chromatogram Start Injection mAU time
  15. 15. 15 Injector Detector Column Solvents Pumps Mixer Chromatogram Start Injection mAU time
  16. 16. 16 Injector Detector Column Solvents Pumps Mixer Chromatogram Start Injection mAU time
  17. 17. 17 Injector Detector Column Solvents Pumps Mixer Chromatogram Start Injection mAU time
  18. 18. 18 Injector Detector Column Solvents Pumps Mixer Chromatogram Start Injection mAU time
  19. 19. 19 Injector Detector Column Solvents Pumps Mixer Chromatogram Start Injection mAU time
  20. 20. 20 Injector Detector Column Solvents Pumps Mixer Chromatogram Start Injection mAU time

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