Chromatography is a technique used to separate mixtures by distributing components between two phases, stationary and mobile. The mixture is dissolved in a mobile phase that carries it through a column containing a stationary phase. Components travel at different rates based on how they partition between the phases, allowing separation. Common chromatography methods include gas chromatography, liquid chromatography, and thin layer chromatography. Chromatography has applications in identifying unknown substances like drugs, proteins, and plant pigments. It was first developed in 1903 and continues to be an important analytical technique.
High performance liquid chromatography (HPLC) head points:
HPLC Advantages Vs GC
Instrumentation
HPLC System
Separations
Mobile Phase Reservoirs
Degasser
Aim of Gradient system
High/Low pressure gradient system
HPLC Pump Criteria
HPLC Pumps: Types
Reciprocating Pumps
Sample introduction
Manual Injector
Auto Injector
HPLC Modes
The Mobile Phase
Hydrophobic interaction
Common reverse phase solvents
Detectors
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High performance liquid chromatography (HPLC) head points:
HPLC Advantages Vs GC
Instrumentation
HPLC System
Separations
Mobile Phase Reservoirs
Degasser
Aim of Gradient system
High/Low pressure gradient system
HPLC Pump Criteria
HPLC Pumps: Types
Reciprocating Pumps
Sample introduction
Manual Injector
Auto Injector
HPLC Modes
The Mobile Phase
Hydrophobic interaction
Common reverse phase solvents
Detectors
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https://www.instagram.com/chaudharypreeti1997/
https://www.facebook.com/profile.php?id=100013419194533
https://twitter.com/preetic27018281
Please like, share, comment and follow.
stay connected
If any query then contact:
chaudharypreeti1997@gmail.com
Thanking-You
Preeti Choudhary
This ppt explained the general desctiption of Chromatography techniques and types of chromatography technique. It also explained the process of Band Broadening .
The Power Point Presentation includes The Size Exclusion Chromatography and Its Method. These Slides may be helpful for master of science students. The Syllabus for the slides was prepared by following as KSV, Gandhinagar. Paper Code is CH-AC-302, Unit-01
Introduction to gas Chromatography
,Principle of gas chromatography
Instrumentation of gas Chromatography
Type of detectors of gas chromatography
Advantages of gas chromatography
Disadvantages of gas chromatography
Applications of gas chromatography
Prezentacja wygłoszona podczas turystycznego #3camp w Gdańsku w dniu 3 czerwca 2014 roku.
Skupiliśmy się w niej na pokazaniu różnic pomiędzy "zwykłą" podróżą, a taką z blogiem. Jak zmienia się rytm pracy, o czym musimy pamiętać, żeby te dwie różne rzeczy dało się przyjaźnie połączyć. Jak dobierać miejsca, do których się udajemy. A także sprzęt, kanały społecznościowe oraz jak ustalić sobie rytm dnia, który będzie pomocny dla samej podróży jak i bloga.
***
Prezentacja różni się od graficznie od tej wygłoszonej - dodaliśmy trochę opisów, żeby nie były tutaj tylko puste zdjęcia. Nie wyświetlają się też filmy.
This ppt explained the general desctiption of Chromatography techniques and types of chromatography technique. It also explained the process of Band Broadening .
The Power Point Presentation includes The Size Exclusion Chromatography and Its Method. These Slides may be helpful for master of science students. The Syllabus for the slides was prepared by following as KSV, Gandhinagar. Paper Code is CH-AC-302, Unit-01
Introduction to gas Chromatography
,Principle of gas chromatography
Instrumentation of gas Chromatography
Type of detectors of gas chromatography
Advantages of gas chromatography
Disadvantages of gas chromatography
Applications of gas chromatography
Prezentacja wygłoszona podczas turystycznego #3camp w Gdańsku w dniu 3 czerwca 2014 roku.
Skupiliśmy się w niej na pokazaniu różnic pomiędzy "zwykłą" podróżą, a taką z blogiem. Jak zmienia się rytm pracy, o czym musimy pamiętać, żeby te dwie różne rzeczy dało się przyjaźnie połączyć. Jak dobierać miejsca, do których się udajemy. A także sprzęt, kanały społecznościowe oraz jak ustalić sobie rytm dnia, który będzie pomocny dla samej podróży jak i bloga.
***
Prezentacja różni się od graficznie od tej wygłoszonej - dodaliśmy trochę opisów, żeby nie były tutaj tylko puste zdjęcia. Nie wyświetlają się też filmy.
Métodos y estrategias de presentación de un producto en mercadotecnia , Ya sea que estés emprendiendo el camino de crear el packaging para el producto que vendes o que estés considerando cambiar el packaging de un producto existente, tal vez te estés preguntando si la apariencia de un empaque es importante. Muchos proveedores de productos piensan que el desempeño de un producto es más importante que la apariencia de su empaque, pero el packaging puede jugar un papel importante en el éxito o fracaso de la venta de un producto.
Gp bullhound-technology-predictions-2015Noyan Ayan
İç mekan odaklı iletişim (iBeacon'lar gibi), sosyal mesajlaşma, dikey pazaryeri gibi trendlerle ilgili isabetli tahminlerine atıfta bulunan GP Bullhound 2015 raporu, bu yıl için tahminlerinde izlenmesi gereken girişimlere de geniş yer ayırıyor.
• Chromatography is a method of separation in which the components to be separated are distributed between two phases, one of these is called a stationary phase and the other is a mobile phase which moves on stationary phase in a definite direction
This presentation was presented when I was MVSc 1st year student, hence this ppt will provide a piece of basic information about the use of Chromatography.in toxicology. Principle of its work and related terminology are explained in this ppt.
NVBDCP.pptx Nation vector borne disease control programSapna Thakur
NVBDCP was launched in 2003-2004 . Vector-Borne Disease: Disease that results from an infection transmitted to humans and other animals by blood-feeding arthropods, such as mosquitoes, ticks, and fleas. Examples of vector-borne diseases include Dengue fever, West Nile Virus, Lyme disease, and malaria.
CDSCO and Phamacovigilance {Regulatory body in India}NEHA GUPTA
The Central Drugs Standard Control Organization (CDSCO) is India's national regulatory body for pharmaceuticals and medical devices. Operating under the Directorate General of Health Services, Ministry of Health & Family Welfare, Government of India, the CDSCO is responsible for approving new drugs, conducting clinical trials, setting standards for drugs, controlling the quality of imported drugs, and coordinating the activities of State Drug Control Organizations by providing expert advice.
Pharmacovigilance, on the other hand, is the science and activities related to the detection, assessment, understanding, and prevention of adverse effects or any other drug-related problems. The primary aim of pharmacovigilance is to ensure the safety and efficacy of medicines, thereby protecting public health.
In India, pharmacovigilance activities are monitored by the Pharmacovigilance Programme of India (PvPI), which works closely with CDSCO to collect, analyze, and act upon data regarding adverse drug reactions (ADRs). Together, they play a critical role in ensuring that the benefits of drugs outweigh their risks, maintaining high standards of patient safety, and promoting the rational use of medicines.
Adv. biopharm. APPLICATION OF PHARMACOKINETICS : TARGETED DRUG DELIVERY SYSTEMSAkankshaAshtankar
MIP 201T & MPH 202T
ADVANCED BIOPHARMACEUTICS & PHARMACOKINETICS : UNIT 5
APPLICATION OF PHARMACOKINETICS : TARGETED DRUG DELIVERY SYSTEMS By - AKANKSHA ASHTANKAR
Title: Sense of Smell
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the primary categories of smells and the concept of odor blindness.
Explain the structure and location of the olfactory membrane and mucosa, including the types and roles of cells involved in olfaction.
Describe the pathway and mechanisms of olfactory signal transmission from the olfactory receptors to the brain.
Illustrate the biochemical cascade triggered by odorant binding to olfactory receptors, including the role of G-proteins and second messengers in generating an action potential.
Identify different types of olfactory disorders such as anosmia, hyposmia, hyperosmia, and dysosmia, including their potential causes.
Key Topics:
Olfactory Genes:
3% of the human genome accounts for olfactory genes.
400 genes for odorant receptors.
Olfactory Membrane:
Located in the superior part of the nasal cavity.
Medially: Folds downward along the superior septum.
Laterally: Folds over the superior turbinate and upper surface of the middle turbinate.
Total surface area: 5-10 square centimeters.
Olfactory Mucosa:
Olfactory Cells: Bipolar nerve cells derived from the CNS (100 million), with 4-25 olfactory cilia per cell.
Sustentacular Cells: Produce mucus and maintain ionic and molecular environment.
Basal Cells: Replace worn-out olfactory cells with an average lifespan of 1-2 months.
Bowman’s Gland: Secretes mucus.
Stimulation of Olfactory Cells:
Odorant dissolves in mucus and attaches to receptors on olfactory cilia.
Involves a cascade effect through G-proteins and second messengers, leading to depolarization and action potential generation in the olfactory nerve.
Quality of a Good Odorant:
Small (3-20 Carbon atoms), volatile, water-soluble, and lipid-soluble.
Facilitated by odorant-binding proteins in mucus.
Membrane Potential and Action Potential:
Resting membrane potential: -55mV.
Action potential frequency in the olfactory nerve increases with odorant strength.
Adaptation Towards the Sense of Smell:
Rapid adaptation within the first second, with further slow adaptation.
Psychological adaptation greater than receptor adaptation, involving feedback inhibition from the central nervous system.
Primary Sensations of Smell:
Camphoraceous, Musky, Floral, Pepperminty, Ethereal, Pungent, Putrid.
Odor Detection Threshold:
Examples: Hydrogen sulfide (0.0005 ppm), Methyl-mercaptan (0.002 ppm).
Some toxic substances are odorless at lethal concentrations.
Characteristics of Smell:
Odor blindness for single substances due to lack of appropriate receptor protein.
Behavioral and emotional influences of smell.
Transmission of Olfactory Signals:
From olfactory cells to glomeruli in the olfactory bulb, involving lateral inhibition.
Primitive, less old, and new olfactory systems with different path
Title: Sense of Taste
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the structure and function of taste buds.
Describe the relationship between the taste threshold and taste index of common substances.
Explain the chemical basis and signal transduction of taste perception for each type of primary taste sensation.
Recognize different abnormalities of taste perception and their causes.
Key Topics:
Significance of Taste Sensation:
Differentiation between pleasant and harmful food
Influence on behavior
Selection of food based on metabolic needs
Receptors of Taste:
Taste buds on the tongue
Influence of sense of smell, texture of food, and pain stimulation (e.g., by pepper)
Primary and Secondary Taste Sensations:
Primary taste sensations: Sweet, Sour, Salty, Bitter, Umami
Chemical basis and signal transduction mechanisms for each taste
Taste Threshold and Index:
Taste threshold values for Sweet (sucrose), Salty (NaCl), Sour (HCl), and Bitter (Quinine)
Taste index relationship: Inversely proportional to taste threshold
Taste Blindness:
Inability to taste certain substances, particularly thiourea compounds
Example: Phenylthiocarbamide
Structure and Function of Taste Buds:
Composition: Epithelial cells, Sustentacular/Supporting cells, Taste cells, Basal cells
Features: Taste pores, Taste hairs/microvilli, and Taste nerve fibers
Location of Taste Buds:
Found in papillae of the tongue (Fungiform, Circumvallate, Foliate)
Also present on the palate, tonsillar pillars, epiglottis, and proximal esophagus
Mechanism of Taste Stimulation:
Interaction of taste substances with receptors on microvilli
Signal transduction pathways for Umami, Sweet, Bitter, Sour, and Salty tastes
Taste Sensitivity and Adaptation:
Decrease in sensitivity with age
Rapid adaptation of taste sensation
Role of Saliva in Taste:
Dissolution of tastants to reach receptors
Washing away the stimulus
Taste Preferences and Aversions:
Mechanisms behind taste preference and aversion
Influence of receptors and neural pathways
Impact of Sensory Nerve Damage:
Degeneration of taste buds if the sensory nerve fiber is cut
Abnormalities of Taste Detection:
Conditions: Ageusia, Hypogeusia, Dysgeusia (parageusia)
Causes: Nerve damage, neurological disorders, infections, poor oral hygiene, adverse drug effects, deficiencies, aging, tobacco use, altered neurotransmitter levels
Neurotransmitters and Taste Threshold:
Effects of serotonin (5-HT) and norepinephrine (NE) on taste sensitivity
Supertasters:
25% of the population with heightened sensitivity to taste, especially bitterness
Increased number of fungiform papillae
- Video recording of this lecture in English language: https://youtu.be/lK81BzxMqdo
- Video recording of this lecture in Arabic language: https://youtu.be/Ve4P0COk9OI
- Link to download the book free: https://nephrotube.blogspot.com/p/nephrotube-nephrology-books.html
- Link to NephroTube website: www.NephroTube.com
- Link to NephroTube social media accounts: https://nephrotube.blogspot.com/p/join-nephrotube-on-social-media.html
These simplified slides by Dr. Sidra Arshad present an overview of the non-respiratory functions of the respiratory tract.
Learning objectives:
1. Enlist the non-respiratory functions of the respiratory tract
2. Briefly explain how these functions are carried out
3. Discuss the significance of dead space
4. Differentiate between minute ventilation and alveolar ventilation
5. Describe the cough and sneeze reflexes
Study Resources:
1. Chapter 39, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 34, Ganong’s Review of Medical Physiology, 26th edition
3. Chapter 17, Human Physiology by Lauralee Sherwood, 9th edition
4. Non-respiratory functions of the lungs https://academic.oup.com/bjaed/article/13/3/98/278874
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Ve...kevinkariuki227
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Verified Chapters 1 - 19, Complete Newest Version.pdf
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Verified Chapters 1 - 19, Complete Newest Version.pdf
Basavarajeeyam is a Sreshta Sangraha grantha (Compiled book ), written by Neelkanta kotturu Basavaraja Virachita. It contains 25 Prakaranas, First 24 Chapters related to Rogas& 25th to Rasadravyas.
2. Definition
Chromatography (from Greek chroma "color and graphein "to write") is
the collective term for a set of laboratory techniques for
the separation of mixtures. The mixture is dissolved in a fluid called
the mobile phase, which carries it through a structure holding
another material called the stationary phase. The various
constituents of the mixture travel at different speeds, causing them
to separate. The separation is based on differential partitioning
between the mobile and stationary phases.
mobile phase = solvent or gas
stationary phase = column packing material
3. Examples of Chromatography
Gas Chromatography
Used to determine the chemical
composition of unknown
substances, such as the different
compounds in gasoline shown by
each separate peak in the graph
below.
Paper Chromatography
Can be used to separate the
components of inks, dyes, plant
compounds (chlorophyll), make-
up, and many other substances
Liquid Chromatography
Used to identify unknown plant
pigments & other compounds.
Thin-Layer Chromatography
Uses thin plastic or glass trays to
identify the composition of pigments,
chemicals, and other unknown
substances.
4. Application of chromatography
• The chromatographic technique is used for the
separation of amino acids, proteins & carbohydrates.
• It is also used for the analysis of drugs, hormones,
vitamins & brain amines.
• Helpful for the qualitative & quantitative analysis of
complex mixtures.
• The technique is also useful for the determination of
molecular weight of proteins.
5. History
• 1903: Michael Tswett (or Tsvet) a Russian botanist separated the pigments in green
plant leaves using a glass column packed with 2 μm inulin and ligroin as mobile phase.
• 1938: Izmailov & Schreiber worked out a procedure where the solid stationary
chromatographic phase is distributed as a thin film on a glass plate.
• 1941: Archer John Porter Martin & Richard Laurence Millington Synge published a
paper on liquid partition chromatography that set the stage for gas liquid
chromatography.
• 1952: Martin and Synge were awarded the Nobel prize in chemistry for the invention
of column partition chromatography, and particularly paper chromatography.
• 1956: Martin & James applied gas chromatography to the separation of acids and
amines.
Michael Tswett Archer John Porter Martin Richard Laurence Millington Synge
6. Methods of chromatography:
Two phases chromatography are:
1. Mobile phase
2. Stationary phase
Classification of chromatographic methods
Chromatography
Mobile Phase Gas Liquid
Stationary
phase Liquid Solid Bonded Liq. Liq Solid Bonded Liq. Ion Ex Polymer
Solid
Process (part’n) (adsorp’n) (adsorp’n- (part’n) (adsorp’n) (adsorp’n- (ion (part’n
part’n) HPLC part’n) exc’ng) sieving)
paper HPLC exclusion
chromat
7. Techniques of chromatography
• Techniques by Chromatographic bed shape
Column chromatography
-The stationary phase is held in to a tube made of glass or metal (gel-ion exchange-
adsorption)
Planar chromatography
Paper chromatography
- A specific type of papers is used as stationary phase.
Thin layer chromatography
- The stationary phase is spread on glass or plastic or aluminum sheets.
• Techniques by Physical state of mobile phase
Gas chromatography
-The mobile phase is an inert gas nitrogen or helium. Again if the stationary phase is
solid it is called: Gas-Solid Chromatography(GSC). When stationary phase is liquid it is
called: Gas-Liquid Chromatography(GLC).
Liquid chromatography
-The mobile phase is liquid. In case of separation by adsorption the stationary phase is
solid so it is called: Liquid-Solid Chromatography(LSC). If separation occurs through
partition, the stationary phase is liquid so it is called: Liquid-Liquid
Chromatography(LLC).
• Affinity chromatography
Supercritical fluid chromatography
8. Mechanisms of separation in chromatography are:
1. ADSORPTION (LIQUID-SOLID) CHROMATOGRAPHY
2. PARTITION (LIQUID-LIQUID) CHROMATOGRAPHY
3. ION-EXCHANGE CHROMATOGRAPHY
4. Molecular Exclusion Chromatography
5. Affinity chromatography
6. HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
1. Adsorption chromatography: It utilizes a mobile liquid or gaseous phase that is adsorbed
onto the surface of a stationary solid phase. The equilibration between the mobile and
stationary phase accounts for the separation of different solutes.
2. Partition chromatography: This form of chromatography is based on a thin film formed
on the surface of a solid support by a liquid stationary phase. Solute equilibrates between the
mobile phase and the stationary liquid.
9. Ion Exchange chromatography : In this type of chromatography the use of a resin (the
stationary solid phase) is used to covalently attach anions or cations onto it. Solute ions of the
opposite charge in the mobile liquid phase are attracted to the resin by electrostatic forces. It
has 2 prinicipal types of ion- exchanger is cationic and anionic.
Molecular Exclusion Chromatography (gel permeation/gel filtration): This type of
chromatography lacks an attractive interaction between the stationary phase and solute. The
liquid or gaseous phase passes through a porous gel which separates the molecules according
to its size. The pores are normally small and exclude the larger solute molecules, but allows
smaller molecules to enter the gel, causing them to flow through a larger volume.
10. Affinity chromatography :
This technique separates proteins on the basis of a reversible interaction between a protein or
group of proteins and a specific ligand coupled to a chromatography matrix. The technique
can be used to separate active bio molecules from denatured or functionally different forms,
to isolate pure substances present at low concentration in large volumes of crude sample and
also to remove specific contaminants.
11. High-performance liquid chromatography (HPLC)
Liquid chromatography (LC) is a separation technique in which the mobile phase is
a liquid. Liquid chromatography can be carried out either in a column or a plane.
Present day liquid chromatography that generally utilizes very small packing
particles and a relatively high pressure is referred to as high performance liquid
chromatography (HPLC).
Uses:
• It has the ability to separate, identify, and quantitate the compounds that are
present in any sample that can be dissolved in a liquid.
• HPLC can be, and has been, applied to just about any sample, such as
pharmaceuticals, food, nutraceuticals, cosmetics, environmental matrices, forensic
12. Chromatography detector
A chromatography detector is a device used in gas chromatography (GC) or liquid
chromatography (LC) to detect components of the mixture being eluted off the
chromatography column. There are two general types of detectors: destructive and
non-destructive. The destructive detectors perform continuous transformation of
the column effluent (burning, evaporation or mixing with reagents) with
subsequent measurement of some physical property of the resulting material
(plasma, aerosol or reaction mixture). The non-destructive detectors are directly
measuring some property of the column effluent (for example UV absorption) and
thus affords for the further analyte recovery.
Destructive detectors
• Flame ionization detector (FID)
• Aerosol-based detector (ABD)
• Flame photometric detector (FPD)
• Atomic-emission detector (AED)
• MS detector
• Nitrogen Phosphorus Detector (NPD)
• Evaporative light scattering detector (ELSD)
13. Non-destructive detectors
• UV detectors : Fixed or variable wavelength, which includes diode array detector (DAD or
PDA). The UV absorption of the effluent is continuously measured at single or multiple
wavelengths. These are by far most popular detectors for LC.
• Thermal conductivity detector (TCD): Measures the thermal conductivity of the effluent.
Only used in GC.
• Fluorescence detector : Irradiates the effluent with a light of set wavelength and measure
the fluorescence of the effluent at a single or multiple wavelength. Used only in LC
• Electron capture detector (ECD): The most sensitive detector known. Allows for the
detection of organic molecules containing halogen, nitro groups etc.
• Conductivity monitor: Continuously measures the conductivity of the effluent. Used only in
LC when conductive eluents (water or alcohols) are used.
• Photoionization detector (PID): Measures the increase in conductivity achieved by ionizing
the effluent gas with UV radiation.
• Refractive index detector (RI or RID): Continuously measures the refractive index of the
effluent. Used only in LC. The lowest sensitivity of all detectors. Useful when nothing else
works and at high analyte concentrations.
• Radio flow detector: Measures radioactivity of the effluent. This detector can be destructive
if a scintillation cocktail is continuously added to the effluent.
• Chiral detector: Continuously measures the optical angle of rotation of the elutant. Used
only in LC when chiral compounds are being analyzed.
14. Rf Factor
Retention Factor
• It is a number that represents how far a compound
travels in a particular solvent.
• It is measured by measuring the ratio of the
distance traveled by the compound and the
distance traveled by the solvent.